A 96-well plate single-well tearable anti-contamination film

Abstract

The utility model discloses a 96 -well plate single -hole tearable anti -pollution pasting film relates to the field of biological experiments, including sealing film, sealing film includes a plurality of single -tearable strip film, a plurality of tearable sealing film are provided on the strip film, adjacent strip film sealing isolation, adjacent sealing film sealing isolation, the applicant experimental staff can only open the sealing film of the target product place pipe mouth, directly uses pipette to draw product and carries out downstream experiment, this process completely avoided the aerosol of puncture sealing film, ensured other hole position sample and the cleanness of experimental environment, greatly reduced the false positive risk, through every independent tearable strip film simultaneously, the strip film is torn off from the hole plate vertically, and then makes the vertical distribution sealing film tear off from the hole plate, through the edge side of the tear -aiding film and the sealing film, tear along the length direction of strip film perpendicular, can make the sealing film whole tear off from the hole plate under the action of tear -aiding film.

Description

Technical Field

[0001] This utility model relates to the field of biological experiments, and in particular to a peelable anti-pollution film for a single well of a 96-well plate. Background Technology

[0002] 96-well plate sealers are laboratory consumables widely used in life sciences, biotechnology, and biological experiments. The film is mainly used to seal the 96-well plate to ensure the cleanliness of the tube openings and to ensure the accuracy and reliability of experimental results.

[0003] When a traditional monolithic sealing film is torn open, all wells are exposed simultaneously, and the amplified products are prone to volatilization, forming aerosols and causing cross-contamination between wells. Summary of the Invention

[0004] The purpose of this invention is to solve the problem of cross-contamination between holes caused by the simultaneous exposure of all holes when the traditional integral sealing film is torn open. Therefore, a 96-hole plate single-hole tearable anti-contamination film is proposed.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: it includes a sealing film, the sealing film comprising multiple individually tearable strip films, the strip films being provided with multiple tearable sealing films, adjacent strip films being sealed and isolated, adjacent sealing films being sealed and isolated, and each sealing film corresponding to the opening of a reaction tube; It also includes a tear-off aid film for peeling off multiple strips of film, the tear-off aid film being adhered to the underside of the sealing film.

[0006] As a further description of the above technical solution: the strip film is provided with a pre-compression straight line, the sealing film is provided with a pre-compression circular line larger than the pipe opening, and the pre-compression straight line and the pre-compression circular line are bonded to the perforated plate through a pressure-sensitive adhesive layer coated on the lower surface.

[0007] As a further description of the above technical solution: the tear-aid film includes multiple reinforcing films arranged perpendicularly to the strip film, and the reinforcing films are bonded to the lower surface of the strip film by a pressure-sensitive adhesive layer.

[0008] As a further description of the above technical solution: the strip film is symmetrically provided with a plurality of linearly distributed easy-tear holes I, and the sealing film is provided with a plurality of annularly distributed easy-tear holes II, the easy-tear holes II being located outside the pre-compression circle.

[0009] As a further description of the above technical solution: multiple reinforcing films are integrally formed with tear-off ears.

[0010] As a further description of the above technical solution: the narrow side of the strip film is integrally formed with a tear-off ear one, and the wide side of the strip film is integrally formed with a tear-off ear two.

[0011] As a further description of the above technical solution: the upper surface of the sealing film is adhered with a tear-off tab bearing a coordinate label.

[0012] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are: This application allows researchers to simply lift the sealing film at the end of the tube containing the target product and directly use a pipette to aspirate the product for downstream experiments. This process completely avoids the aerosol generated by puncturing the sealing film, ensuring the cleanliness of other well samples and the experimental environment, and greatly reducing the risk of false positives. At the same time, by tearing each individually torn strip vertically from the well plate, the vertically distributed sealing film can be torn off the well plate. By tearing along the edge of the tear aid film and the sealing film perpendicular to the length of the strip, the sealing film can be completely torn off the well plate with the help of the tear aid film. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This utility model Figure 1 Enlarged structural diagram at point A in the middle; Figure 3 This is a schematic diagram of the sealing membrane structure of this utility model; Figure 4 This utility model Figure 3 Enlarged structural diagram at point B; Figure 5 This is a schematic diagram of the overall lower surface structure of this utility model; Figure 6 This is a schematic diagram of the sealing film structure of this utility model; Figure 7 This is a schematic diagram of the tear-aid film structure of this utility model; Figure 8 This is a schematic diagram of the sealing membrane sealing orifice plate structure of this utility model.

[0014] Legend: 10. Sealing film; 11. Strip film; 111. Easy-tear hole one; 112. Tear-off ear one; 113. Tear-off ear two; 114. Film opening; 12. Sealing film; 121. Easy-tear hole two; 122. Tear-off ear three; 13. Pre-compression circle; 14. Pre-compression straight line; 20. Tear-aid film; 21. Tear-off ear four; 22. Reinforcing film. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] like Figure 1 - Figure 8 As shown, the present invention provides a 96-well plate single-well tearable anti-contamination film, including a sealing film 10, which includes twelve individually tearable strips 11, and eight tearable sealing films 12 on each strip 11. Adjacent strips 11 are sealed and isolated, and adjacent sealing films 12 are sealed and isolated. Each sealing film 12 corresponds to the opening of a reaction tube. The experimenter can simply lift the sealing film 12 of the tube opening where the target product is located and directly use a pipette to draw the product for downstream experiments. This process completely avoids the aerosol generated by puncturing the sealing film, ensuring the cleanliness of other well samples and the experimental environment, and greatly reducing the risk of false positives. At the same time, the twelve strip films 11 are arranged horizontally, and the strip films 11 are torn vertically from the 96-hole plate through each independently torn strip film 11, thereby causing the vertically distributed sealing film 12 to be torn off from the perforated plate; It also includes a tear-aid film 20 for peeling off multiple strip films 11. The tear-aid film 20 is attached to the lower surface of the sealing film 10. When it is necessary to peel off the entire sealing film 10, the sealing film 10 can be peeled off from the perforated plate by tearing along the length direction perpendicular to the strip film 11 through the edge side of the tear-aid film 20 and the edge side of the sealing film 10.

[0017] like Figure 2 , Figure 6 As shown, the strip membrane 11 is provided with a pre-compression straight line 14, and the sealing membrane 12 is provided with a pre-compression circular line 13 larger than the tube opening. The pre-compression straight line 14 and the pre-compression circular line 13 are bonded to the orifice plate through a pressure-sensitive adhesive layer coated on the lower surface to ensure independent airtight sealing of each reaction tube. At the same time, adjacent strip membranes 11 are bonded to the orifice plate through the pressure-sensitive adhesive layer at the bottom of the pre-compression straight line 14 to ensure that adjacent strip membranes 11 are not affected when the strip membrane 11 is torn.

[0018] like Figure 3 , Figure 7 As shown, the tear-aid membrane 20 includes multiple reinforcing membranes 22 arranged perpendicularly to the strip membrane 11. The reinforcing membranes 22 are bonded to the lower surface of the strip membrane 11 by a pressure-sensitive adhesive layer, and the reinforcing membranes 22 are placed on the perforated plate between the tube openings, and the lower surface of the reinforcing membranes 22 is bonded to the perforated plate by a pressure-sensitive adhesive layer.

[0019] The strip film 11 has multiple linearly distributed easy-tear holes 111, and the sealing film 12 has multiple annularly distributed easy-tear holes 121. The easy-tear holes 121 are located outside the pre-compression circle 13. The easy-tear holes 111 and 121 are laser-etched weakening lines, so that when tearing a single strip film 11 or sealing film 12, the cut is neat and it is not easy to involve adjacent strip films 11 or sealing films 12.

[0020] like Figure 7 As shown, multiple reinforcing films 22 are integrally formed with tear-off ears 21. The reinforcing films 22 and tear-off ears 21 are integrally formed by cutting, making them easy to pick up with fingers.

[0021] like Figure 5 As shown, the narrow side of the strip film 11 is integrally formed with a tear-off ear 112, and the wide side of the strip film 11 is integrally formed with a tear-off ear 213. The tear-off ear 112, the tear-off ear 213 and the strip film 11 are integrally formed by cutting. The tear-off ear 112 is used to make it easy to tear off the individual strip film 11 vertically. The tear-off ear 213 is integrally formed on the outer edge of the outermost strip film 11 and is placed above the tear-off ear 421. Then, by pinching the tear-off ear 213 and the tear-off ear 421 at the same time, the entire sealing film 10 can be torn off horizontally from the perforated plate.

[0022] like Figure 1 , Figure 6 , Figure 8 As shown, a tear-off tab 122 with coordinate labels is adhered to the upper surface of the sealing film 12. The label can be used as a writing area to mark sample information or coordinates for easy identification. At the same time, the tear-off tab 122 makes it easy to tear the sealing film 12 off the strip film 11 and form a film opening 114 corresponding to the tube opening.

[0023] Working principle: After the real-time fluorescence quantitative PCR reaction is completed, the experimenter can use the tear tab 3 122 to lift only the sealing film 12 of the well where the target product is located, so that the sealing film 12 can be torn off from the strip film 11 along the easy-tear hole 2 121, and the product can be directly aspirated by pipette for downstream experiments. By tearing the ear 112, the experimenters can lift the strip film 11 in the vertical direction, and then completely tear off the sealing film 12 of the hole where the target product is located in the vertical direction. Experimenters can also tear the entire sealing membrane 10 off the perforated plate by simultaneously pinching the fourth tearing lug 21 and the second tearing lug 113, and then tearing it from right to left along the transverse direction under the action of the reinforcing membrane 22.

[0024] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A 96-well plate single-hole peelable anti-pollution film, characterized in that: Includes a sealing film (10), the sealing film (10) includes a plurality of individually tearable strip films (11), the strip films (11) are provided with a plurality of tearable sealing films (12), adjacent strip films (11) are sealed and isolated, adjacent sealing films (12) are sealed and isolated, and each sealing film (12) corresponds to the opening of a reaction tube; It also includes a tear-off aid film (20) for peeling off multiple strip films (11), the tear-off aid film (20) being adhered to the lower surface of the sealing film (10).

2. The 96-well plate single-hole peelable anti-pollution film according to claim 1, characterized in that, The strip film (11) is provided with a pre-compression straight line (14), and the sealing film (12) is provided with a pre-compression circular line (13) larger than the pipe opening. The pre-compression straight line (14) and the pre-compression circular line (13) are bonded to the orifice plate through a pressure-sensitive adhesive layer coated on the lower surface.

3. The 96-well plate single-hole peelable anti-pollution film according to claim 1, characterized in that, The tear-aid film (20) includes multiple reinforcing films (22) arranged perpendicularly to the strip film (11), and the reinforcing films (22) are bonded to the lower surface of the strip film (11) by pressure-sensitive adhesive.

4. A 96-hole plate single-hole peelable anti-pollution film according to claim 2, characterized in that, The strip film (11) has multiple linearly distributed easy-tear holes (111) symmetrically arranged, and the sealing film (12) has multiple annularly distributed easy-tear holes (121) arranged, with the easy-tear holes (121) located outside the pre-pressed circular line (13).

5. A 96-well plate single-hole peelable anti-pollution film according to claim 3, characterized in that, Multiple of the aforementioned reinforcing membranes (22) are integrally formed with four tear-off tabs (21).

6. The 96-well plate single-hole peelable anti-pollution film according to claim 1, characterized in that, The narrow side of the strip film (11) is integrally formed with a tear-off ear one (112), and the wide side of the strip film (11) is integrally formed with a tear-off ear two (113).

7. A 96-well plate single-hole peelable anti-pollution film according to claim 1, characterized in that, The upper surface of the sealing film (12) is adhered with a tear-off tab (122) bearing a coordinate label.

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