Magnetic bead separation device for nucleic acid extraction

Abstract

The utility model relates to experimental apparatus technical field, concretely is a kind of magnetic bead separation device for nucleic acid extraction, including shell, the inside installation of shell has electric push rod, the output of electric push rod penetrates shell, the telescopic end of electric push rod is fixedly connected with second connecting plate, the bottom of second connecting plate is uniformly fixedly connected with multiple magnetic bar, the bottom of shell is fixedly connected with first connecting plate, the side of first connecting plate is fixedly connected with third connecting plate, the upper surface of third connecting plate is uniformly equipped with multiple through-hole, the inboard wall of through-hole is fixedly connected with stainless steel spring piece, the side of stainless steel spring piece is fixedly connected with sleeve, the linkage design of the utility model vibration motor and sleeve, the adhesion of magnetic bead and sleeve is broken by high-frequency mechanical vibration (adjustable frequency), it is proved by experiment, and magnetic bead residual rate greatly reduces, and nucleic acid recovery rate is greatly improved, significantly better than existing equipment, improve work efficiency.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment technology, specifically to a magnetic bead separation device for nucleic acid extraction. Background Technology

[0002] In nucleic acid extraction, the magnetic bead method is widely used due to its simplicity and high extraction efficiency. Its core principle is to utilize the functional groups on the surface of magnetic beads to specifically bind to nucleic acids, achieving nucleic acid separation through magnetic field adsorption-transfer-elution. However, existing magnetic bead separation devices suffer from the following technical challenges:

[0003] High magnetic bead residue rate: Traditional magnetic bead separation relies on the magnetic rod adsorption and natural detachment. Magnetic beads are easily attached to the inner wall of the container due to static electricity or surface tension, and the residue rate often exceeds 5%, which leads to a decrease in nucleic acid recovery rate.

[0004] Low separation efficiency: Manual shaking or static elution takes a long time (3-5 minutes per batch), and uneven force can easily cause magnetic beads to aggregate. Therefore, a magnetic bead separation device for nucleic acid extraction is proposed. Utility Model Content

[0005] In view of this, the present invention provides a magnetic bead separation device for nucleic acid extraction to solve or alleviate the technical problems existing in the prior art, and at least provides a beneficial option.

[0006] The technical solution of this utility model is implemented as follows: A magnetic bead separation device for nucleic acid extraction includes a housing, an electric push rod installed inside the housing, the output end of the electric push rod penetrating through the housing, a second connecting plate fixedly connected to the telescopic end of the electric push rod, a plurality of magnetic rods uniformly fixedly connected to the bottom of the second connecting plate, a first connecting plate fixedly connected to the bottom of the housing, a third connecting plate fixedly connected to one side of the first connecting plate, a plurality of through holes uniformly opened on the upper surface of the third connecting plate, a stainless steel spring sheet fixedly connected to the inner side wall of the through holes, a sleeve fixedly connected to one side of the stainless steel spring sheet, and a vibration motor installed on the outer side wall of the sleeve.

[0007] More preferably, a battery is installed on the inner sidewall of the housing.

[0008] More preferably, a handle is installed on the outer side wall of the housing.

[0009] More preferably, a controller is mounted on one side of the housing.

[0010] More preferably, the telescopic end of the electric push rod is fitted with a guide ring, and the outer wall of the guide ring is fixedly connected to the first connecting plate.

[0011] The present invention has the following advantages due to the adoption of the above technical solution:

[0012] The linkage design of the vibration motor and the sleeve in this invention breaks the adhesion between the magnetic bead and the sleeve through high-frequency mechanical vibration (adjustable frequency). Experimental verification shows that the magnetic bead residue rate is greatly reduced, the nucleic acid recovery rate is significantly improved, which is significantly better than existing equipment and improves work efficiency.

[0013] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a structural diagram of the present invention;

[0016] Figure 2 This is a cross-sectional structural diagram of the shell of this utility model.

[0017] Reference numerals: 1. Housing; 2. Electric push rod; 3. Battery; 4. Handle; 5. Controller; 6. First connecting plate; 7. Guide ring; 8. Second connecting plate; 9. Magnetic rod; 10. Third connecting plate; 11. Through hole; 12. Stainless steel spring; 13. Sleeve; 14. Vibration motor. Detailed Implementation

[0018] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0019] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0020] like Figure 1-2As shown, this utility model embodiment provides a magnetic bead separation device for nucleic acid extraction, including a housing 1. An electric push rod 2 is installed inside the housing 1. The output end of the electric push rod 2 passes through the housing 1. A second connecting plate 8 is fixedly connected to the telescopic end of the electric push rod 2. A plurality of magnetic rods 9 are uniformly fixedly connected to the bottom of the second connecting plate 8. A first connecting plate 6 is fixedly connected to the bottom of the housing 1. A third connecting plate 10 is fixedly connected to one side of the first connecting plate 6. A plurality of through holes 11 are uniformly opened on the upper surface of the third connecting plate 10. A stainless steel spring sheet 12 is fixedly connected to the inner side wall of the through hole 11. A sleeve 13 is fixedly connected to one side of the stainless steel spring sheet 12. A vibration motor 14 is installed on the outer side wall of the sleeve 13.

[0021] In one embodiment, a battery 3 is installed on the inner sidewall of the housing 1.

[0022] In one embodiment, a handle 4 is mounted on the outer side wall of the housing 1.

[0023] In one embodiment, a controller 5 is mounted on one side of the housing 1.

[0024] In one embodiment, the telescopic end of the electric push rod 2 is fitted with a guide ring 7, and the outer wall of the guide ring 7 is fixedly connected to the first connecting plate 6.

[0025] In operation, the following steps are taken: the controller 5 activates the electric push rod 2, whose telescopic end descends vertically along the guide ring 7, causing the magnetic rod 9 at the bottom of the second connecting plate 8 to be inserted into the sleeve 13; multiple sleeves 13 are inserted into the deep-well plate containing nano-magnetic beads, and the magnetic rod 9 generates a magnetic field to adsorb the magnetic bead-nucleic acid complex. After maintaining this for 10-20 seconds, when the sleeve 13 carrying the magnetic beads is moved above the target elution tube, the controller 5 controls the electric push rod 2 to move the magnetic rod 9 upward, releasing the adsorption of the magnetic beads. At the same time, the vibration motor 14 is activated, causing the sleeve 13 and the internal elution tube to vibrate at high frequency; the mechanical force generated by the vibration breaks the adsorption force between the magnetic beads and the sleeve 13, causing the magnetic beads to quickly fall into the elution solution.

[0026] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this utility model, and these should all be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A magnetic bead separation device for nucleic acid extraction, characterized in that: The device includes a housing (1), an electric push rod (2) is installed inside the housing (1), the output end of the electric push rod (2) passes through the housing (1), the telescopic end of the electric push rod (2) is fixedly connected to a second connecting plate (8), a plurality of magnetic rods (9) are evenly fixedly connected to the bottom of the second connecting plate (8), a first connecting plate (6) is fixedly connected to the bottom of the housing (1), a third connecting plate (10) is fixedly connected to one side of the first connecting plate (6), a plurality of through holes (11) are evenly opened on the upper surface of the third connecting plate (10), a stainless steel spring sheet (12) is fixedly connected to the inner side wall of the through hole (11), a sleeve (13) is fixedly connected to one side of the stainless steel spring sheet (12), and a vibration motor (14) is installed on the outer side wall of the sleeve (13).

2. The magnetic bead separation device for nucleic acid extraction according to claim 1, characterized in that: A battery (3) is installed on the inner wall of the housing (1).

3. The magnetic bead separation device for nucleic acid extraction according to claim 1, characterized in that: A handle (4) is installed on the outer side wall of the housing (1).

4. The magnetic bead separation device for nucleic acid extraction according to claim 1, characterized in that: A controller (5) is installed on one side of the housing (1).

5. The magnetic bead separation device for nucleic acid extraction according to claim 1, characterized in that: The telescopic end of the electric push rod (2) is fitted with a guide ring (7), and the outer side wall of the guide ring (7) is fixedly connected to the first connecting plate (6).

Images