A digestion kit for organoid and stem cell culture

Abstract

This invention discloses a digestion kit for organoid and stem cell culture, applicable to the field of microbiology devices. The centrifuge tubes are held in place by a rotating cylinder and a central rubber sleeve. The lower cover and rotating cylinder are driven by an outer and inner prismatic cylinder, enabling rotational mixing of the centrifuge tubes. Compared to manually holding and shaking the tubes, this method reduces the probability of damage after the tubes are released and makes mixing easier. It also improves the mixing effect of organ tissues and digestive agents within the tubes even without power. Furthermore, a squeezing cylinder located inside the upper cover compresses the centrifuge tubes and storage tubes, vertically limiting their movement and reducing shaking and collisions during transport. Combined with upper and lower sealing rings, this improves the overall sealing of the kit and further enhances reagent preservation.

Description

Technical Field

[0001] This invention relates to the field of microbiological devices, and in particular to a digestion kit for organoid and stem cell culture. Background Technology

[0002] Organoids, as three-dimensional tissue analogs formed by stem cell differentiation, can highly replicate the structure and function of organs in the body and have become a core tool for disease modeling, drug screening and regenerative medicine research. One of the key steps in their culture process is the digestion and separation of organ tissues. The digestion kit for stem cell culture achieves efficient and safe cell acquisition by precisely regulating the action mechanism of digestive enzymes. The digestion kit generally contains a variety of digestive agents, which need to be injected into the organ tissue for mixing during use.

[0003] The existing patent with publication number CN220183205U discloses a digestion kit for skin fibroblasts. By setting a slot to initially limit the test tube, the spring on the inner wall of the slot cooperates with the semi-circular rubber block. Utilizing the principle of friction and the magnetic principle of mutual attraction between the magnets on the inner side of the block, the test tube is fixed more stably and flexibly, realizing reliable fixation of the test tube during the use of the digestion kit.

[0004] The existing patent with publication number CN208018648U discloses a reagent kit for gastroenterology testing. It constructs a test tube placement device by means of a box body, a box cover and a fixing seat with fixing holes and grooves, and a shaking transmission device constructed with a drive shaft, etc., so that the reagent kit can automatically shake, thereby improving the efficiency of gastroenterology testing and solving the problem of low efficiency in traditional testing.

[0005] While existing technologies have disclosed solutions for stabilizing test tubes through friction and magnetic attraction, as well as technologies for automatically shaking reagents and improving mixing by using a shaking transmission device, there are still significant shortcomings: reagent kits equipped with shaking mechanisms are bulky and dependent on power, while manual shaking can easily lead to loss of hand and damage, making it difficult to meet the needs of mass production and on-site use. Utility Model Content

[0006] The core of this invention lies in solving the problem of easy slippage when manually holding and shaking test tubes in the prior art by using a lower cover that is linked to the centrifuge tube. Furthermore, the lower cover and the cylinder are slidably connected by an arc-shaped plate, reducing the difficulty of mixing operations.

[0007] To solve the above problems, the present invention adopts the following technical solution.

[0008] A digestion kit for organoid and stem cell culture includes a cylindrical body with a top cover threaded to the upper end. A top plate and a bottom plate are fixedly connected to the inner wall of the cylindrical body in parallel. A liquid storage tube is inserted into the top plate near the edge, and the liquid storage tube is filled with digestive agent. The lower end of the liquid storage tube passes through the bottom plate and extends below the bottom plate.

[0009] A rotating cylinder is rotatably connected to the center of both the loading plate and the download plate. A central rubber sleeve is fixedly connected to the inside of the rotating cylinder, and a centrifuge tube is inserted into the central rubber sleeve. An inner prismatic cylinder is fixedly connected to the lower end of the rotating cylinder on the download plate. An outer prismatic cylinder is slidably sleeved on the outside of the inner prismatic cylinder. A lower cover is fixedly connected to the lower end of the outer prismatic cylinder, and the lower cover is threadedly connected to the lower end of the cylinder.

[0010] Furthermore, both the upper plate and the lower plate are disc-shaped structures. The upper plate has a circumferentially evenly distributed side hole 1, and the lower plate has a side hole 2 opposite to the side hole 1. Side rubber sleeves are fixedly connected to both side hole 1 and side hole 2. The side rubber sleeves are cylindrical structures with an I-shaped cross-section. A cylindrical insertion hole 1 is opened at the center of the side rubber sleeve. The diameter of the insertion hole 1 is smaller than the outer diameter of the liquid storage tube.

[0011] Furthermore, a central hole one is opened at the center of the upper plate, and a central hole two is opened at the center of the lower plate. A rotating cylinder is rotatably connected inside both central holes one and two. The rotating cylinder is a cylindrical structure with an I-shaped cross-section. A columnar hole for accommodating the central rubber sleeve is opened at its center. The central rubber sleeve has the same structure as the side rubber sleeve. A insertion hole two for accommodating centrifuge tubes is opened at the center of the central rubber sleeve. The diameter of the insertion hole two is smaller than the outer diameter of the centrifuge tubes.

[0012] Furthermore, the centrifuge tube includes a test tube body, a threaded cap connected to the upper end of the test tube body, and a protruding ring fixedly connected to the side wall of the test tube body, which abuts against the lower end of the threaded cap. The centrifuge tube and the liquid storage tube have the same structure.

[0013] Furthermore, the cylinder is a hollow cylindrical structure with openings at both the top and bottom. The centrifuge tube, the liquid storage tube, and the cylinder are all made of transparent material, which is one of polymethyl methacrylate, polystyrene, and polycarbonate.

[0014] Furthermore, a pair of arc-shaped plates are provided on the upper part of the lower cover, and a slider is fixedly connected to the lower end of the arc-shaped plates. A radial groove is provided on the upper surface of the lower cover for the slider to slide. A guide rod that passes through the slider is fixedly connected to the inner wall of the radial groove. A spring is sleeved on the guide rod and abuts against the inner end of the slider. An annular groove is provided on the lower part of the inner wall of the cylinder for the arc-shaped plates to slide vertically.

[0015] Furthermore, both the inner and outer prismatic tubes are cylindrical structures with a regular polygonal cross-section, the annular groove is an annular groove with a rectangular cross-section, and the arc plate is an arc block with an inverted L-shaped cross-section.

[0016] Furthermore, a squeezing cylinder is slidably connected to the inside of the top cover. The lower end of the squeezing cylinder abuts against the upper end of the centrifuge tube and the liquid storage tube. A second spring abuts against the inner wall of the squeezing cylinder, and the upper end of the second spring abuts against the inner wall of the top cover.

[0017] Furthermore, the lower end of the upper cover has an annular groove three for the vertical sliding of the extrusion cylinder, and the upper cover has an annular groove two outside the annular groove three. An upper sealing ring is fixedly connected inside the annular groove two. An annular groove four is opened on the lower cover, and a lower sealing ring is fixedly connected inside the annular groove four.

[0018] Compared with existing technologies, the advantages of this utility model are:

[0019] (1) This utility model clamps the centrifuge tube by rotating the cylinder and the central rubber sleeve, and realizes the transmission between the lower cover and the rotating cylinder by the outer prismatic cylinder and the inner prismatic cylinder, so as to realize the rotation and mixing of the centrifuge tube. Compared with the method of directly holding the test tube manually and shaking it, it reduces the probability of the test tube being damaged after being released from the hand, and the mixing operation is more labor-saving. At the same time, it improves the mixing effect of organs, tissues and digestive agents in the test tube under the condition of no power supply.

[0020] (2) This utility model uses a squeezing cylinder set inside the top cover to squeeze the centrifuge tube and the liquid storage tube, thereby achieving vertical positioning of the centrifuge tube and the liquid storage tube, reducing the shaking of the centrifuge tube and the liquid storage tube during transportation, and improving the overall sealing of the reagent kit and the preservation effect of the reagent by using the upper sealing ring and the lower sealing ring. Attached Figure Description

[0021] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0022] Figure 2 This is a schematic diagram of the transverse cross-sectional structure of the present invention;

[0023] Figure 3 This is a schematic diagram of the exploded structure of this utility model;

[0024] Figure 4 This is a schematic diagram of the exploded structure of the centrifuge tube in this utility model;

[0025] Figure 5 This is a schematic diagram of the assembly structure of the upper loading plate and the lower loading plate in this utility model;

[0026] Figure 6 This is a schematic diagram of the assembly structure of the arc-shaped plate and the lower cover in this utility model;

[0027] Figure 7 This is a schematic diagram showing the lower cover and the cylinder body detached in this utility model;

[0028] Figure 8 This is a schematic diagram showing the state in which the lower cover drives the centrifuge tube to rotate in this utility model.

[0029] Figure 9 This is a cross-sectional exploded view of the upper cover in this utility model.

[0030] The following are the labeling instructions in the diagram: 1. Cylinder body; 101. Annular groove one; 2. Top cover; 201. Annular groove two; 202. Annular groove three; 3. Bottom cover; 301. Annular groove four; 302. Radial slide groove; 4. Upper loading plate; 401. Side hole one; 402. Center hole one; 5. Download plate; 501. Side hole two; 502. Center hole two; 6. Centrifuge tube; 601. Test tube body; 602. Threaded cap; 603. Raised ring; 7. Central rubber sleeve; 8. Rotating cylinder; 9. Liquid storage tube; 10. Inner prismatic cylinder; 11. Outer prismatic cylinder; 12. Arc plate; 13. Sliding block; 14. Guide rod; 15. Spring one; 16. Lower sealing ring; 17. Upper sealing ring; 18. Squeezing cylinder; 19. Spring two; 20. Side rubber sleeve. Detailed Implementation

[0031] The technical solution will now be clearly and completely described with reference to the accompanying drawings in the embodiments of this utility model.

[0032] First implementation method

[0033] Please see Figures 1-9 In one embodiment of the present invention, a digestion kit for organoid and stem cell culture includes a cylindrical body 1, with a top cover 2 threadedly connected to the upper end of the cylindrical body 1. A top plate 4 and a bottom plate 5 are fixedly connected to the inner wall of the cylindrical body 1 in parallel. A liquid storage tube 9 is inserted into the upper plate 4 near the edge and is evenly distributed in a circle. The liquid storage tube 9 is filled with digestive agent. The lower end of the liquid storage tube 9 passes through the bottom plate 5 and extends below the bottom plate 5.

[0034] Please see Figure 2 and Figure 3 A rotating cylinder 8 is rotatably connected to the center of both the upper plate 4 and the lower plate 5. A central rubber sleeve 7 is fixedly connected to the inner side of the rotating cylinder 8. A centrifuge tube 6 is inserted into the central rubber sleeve 7. An inner prismatic cylinder 10 is fixedly connected to the lower end of the rotating cylinder 8 located on the lower plate 5. An outer prismatic cylinder 11 is slidably sleeved on the outer side of the inner prismatic cylinder 10. A lower cover 3 is fixedly connected to the lower end of the outer prismatic cylinder 11. The lower cover 3 is threadedly connected to the lower end of the cylinder 1.

[0035] Specifically, the usage includes the following steps:

[0036] Step 1: Open the top cover 2, take out the centrifuge tube 6 and the storage tube 9, put the organ tissue into the centrifuge tube 6, and pour the digestive agent in the storage tube 9 into the centrifuge tube 6 as needed;

[0037] Step 2: Reinsert the centrifuge tube 6 into the central rubber sleeve 7;

[0038] Step 3: The operator holds the outer wall of the cylinder 1 with one hand and screws the lower cover 3 off the cylinder 1 with the other hand, while keeping the outer prismatic cylinder 11 in contact with the inner prismatic cylinder 10. Then, the lower cover 3 is rotated, and the lower cover 3 drives the inner prismatic cylinder 10 to rotate through the outer prismatic cylinder 11. The inner prismatic cylinder 10 drives the rotating cylinder 8 located on the download plate 5 to rotate. The rotating cylinder 8 drives the centrifuge tube 6 to rotate through the central rubber sleeve 7, thus rotating and mixing the centrifuge tube 6, so that the organ tissue and digestive agent in the centrifuge tube 6 are fully mixed.

[0039] Compared to traditional digestion kits, this invention uses a rotating cylinder 8 and a central rubber sleeve 7 to clamp the centrifuge tube 6, and uses an outer prismatic cylinder 11 and an inner prismatic cylinder 10 to drive the lower cover 3 and the rotating cylinder 8, thereby achieving rotational mixing of the centrifuge tube 6. Compared to the method of directly holding the test tube manually and shaking it, this reduces the probability of damage after the test tube is released, and the mixing operation is more labor-saving. At the same time, it improves the mixing effect of organs, tissues and digestive agents in the test tube under conditions without power supply.

[0040] Please see Figure 2 and Figure 5 Both the upper plate 4 and the lower plate 5 are disc-shaped structures. The upper plate 4 has a circumferentially evenly distributed side hole 401. The lower plate 5 has a side hole 501 opposite to the side hole 401. A side rubber sleeve 20 is fixedly connected inside both the side hole 401 and the side hole 501. The side rubber sleeve 20 is a cylindrical structure with an I-shaped cross-section. A cylindrical insertion hole 1 is opened at the center of the side rubber sleeve 20. The diameter of the insertion hole 1 is smaller than the outer diameter of the liquid storage tube 9.

[0041] Specifically, by setting the side rubber sleeve 20 in the side hole 1 401 and the side hole 2 501, the liquid storage tube 9 is interference-fitted with the side rubber sleeve 20 to achieve clamping and fixing of the liquid storage tube 9.

[0042] Please see Figure 2 and Figure 5The upper plate 4 has a central hole 402 at its center, and the lower plate 5 has a central hole 502 at its center. A rotating cylinder 8 is rotatably connected inside both the central hole 402 and the central hole 502. The rotating cylinder 8 is a cylindrical structure with an I-shaped cross-section. A columnar hole for accommodating the central rubber sleeve 7 is opened at its center. The central rubber sleeve 7 has the same structure as the side rubber sleeve 20. A second insertion hole for accommodating the centrifuge tube 6 is opened at the center of the central rubber sleeve 7. The diameter of the second insertion hole is smaller than the outer diameter of the centrifuge tube 6.

[0043] Specifically, after the centrifuge tube 6 is inserted into the central rubber sleeve 7, the centrifuge tube 6 and the central rubber sleeve 7 are interference-fitted to clamp and fix the centrifuge tube 6, while facilitating the removal and placement of the centrifuge tube 6.

[0044] Please see Figure 2 and Figure 4 The centrifuge tube 6 includes a tube body 601, with a threaded cap 602 threadedly connected to the upper end of the tube body 601. A protruding ring 603 that abuts against the lower end of the threaded cap 602 is fixedly connected to the side wall of the tube body 601. The centrifuge tube 6 and the liquid storage tube 9 have the same structure.

[0045] Specifically, the centrifuge tube 6 and the liquid storage tube 9, which are equipped with a raised ring 603, have a good limiting effect when they are inserted, which makes it easier for the operator to quickly perceive the insertion position of the centrifuge tube 6 and the liquid storage tube 9, and improves the fixing effect.

[0046] In this embodiment, the cylinder 1 is a hollow cylindrical structure with openings at both the top and bottom. The centrifuge tube 6, the liquid storage tube 9, and the cylinder 1 are all made of transparent material, which is one of polymethyl methacrylate, polystyrene, and polycarbonate, so that personnel can easily observe the state of the solution in the centrifuge tube 6 and the liquid storage tube 9.

[0047] Please see Figure 2 and Figure 6 A pair of arc-shaped plates 12 are provided above the lower cover 3. A slider 13 is fixedly connected to the lower end of the arc-shaped plates 12. A radial groove 302 for sliding the slider 13 is provided on the upper surface of the lower cover 3. A guide rod 14 that passes through the slider 13 is fixedly connected to the inner wall of the radial groove 302. A spring 15 that abuts against the inner end of the slider 13 is sleeved on the guide rod 14. An annular groove 101 for vertical sliding of the arc-shaped plates 12 is provided on the lower part of the inner wall of the cylinder 1.

[0048] Please see Figure 2 and Figure 3 Both the inner prismatic tube 10 and the outer prismatic tube 11 are cylindrical structures with a regular polygonal cross-section. The annular groove 101 is an annular groove with a rectangular cross-section. The arc plate 12 is an arc block with an inverted L-shaped cross-section.

[0049] Specifically, during the process of unscrewing the lower cover 3, the arc plate 12 slides downward in the annular groove 101. After the lower cover 3 is completely separated from the cylinder 1, the pair of arc plates 12 cause the lower cover 3 to hang below the opening at the lower end of the cylinder 1. At this time, the outer prismatic cylinder 11 and the inner prismatic cylinder 10 remain in contact, eliminating the need to manually maintain the contact between the outer prismatic cylinder 11 and the inner prismatic cylinder 10, thus reducing the difficulty of operation. In addition, when it is necessary to disassemble the lower cover 3, the pair of arc plates 12 are pushed by hand to move them in opposite directions, thereby causing the arc plates 12 to detach from the cylinder 1, thus realizing the disassembly of the lower cover 3.

[0050] Second implementation method

[0051] Based on the first implementation, please refer to Figure 2 and Figure 9 A squeezing cylinder 18 is slidably connected to the inner side of the upper cover 2. The lower end of the squeezing cylinder 18 abuts against the upper end of the centrifuge tube 6 and the liquid storage tube 9. A spring 19 is abutted against the inner wall of the squeezing cylinder 18. The upper end of the spring 19 abuts against the inner wall of the upper cover 2.

[0052] Specifically, the centrifuge tube 6 and the liquid storage tube 9 are squeezed by the squeezing cylinder 18 set inside the upper cover 2, so that the centrifuge tube 6 and the liquid storage tube 9 are fixed in the vertical direction when stored in the cylinder 1, reducing the shaking of the centrifuge tube 6 and the liquid storage tube 9 during transportation and improving the protection effect of the centrifuge tube 6 and the liquid storage tube 9.

[0053] Please see Figure 2 The lower end of the upper cover 2 is provided with an annular groove 3 202 for the extrusion cylinder 18 to slide vertically. The upper cover 2 is provided with an annular groove 201 outside the annular groove 3 202. An upper sealing ring 17 is fixedly connected in the annular groove 201. The lower cover 3 is provided with an annular groove 4 301. A lower sealing ring 16 is fixedly connected in the annular groove 4 301.

[0054] Specifically, the lower sealing ring 16 and the upper sealing ring 17 improve the sealing of the reagent kit, thereby enhancing the overall sealing performance and improving the preservation effect.

[0055] The above description is merely a preferred embodiment of this utility model; however, the protection scope of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in this utility model, based on the technical solution and its improved concept, should be included within the protection scope of this utility model.

Claims

1. A digestion kit for organoid and stem cell culture, characterized in that, Includes a cylinder (1), the upper end of which is threadedly connected to a top cover (2), and the inner wall of the cylinder (1) is fixedly connected to a parallel upper loading plate (4) and a lower loading plate (5). The upper loading plate (4) has a liquid storage tube (9) evenly distributed in a circle near its edge. The liquid storage tube (9) is filled with a digestive agent. The lower end of the liquid storage tube (9) passes through the lower loading plate (5) and extends to the bottom of the lower loading plate (5). A rotating cylinder (8) is rotatably connected to the center of both the upper plate (4) and the lower plate (5). A central rubber sleeve (7) is fixedly connected to the inner side of the rotating cylinder (8). A centrifuge tube (6) is inserted into the central rubber sleeve (7). An inner prismatic cylinder (10) is fixedly connected to the lower end of the rotating cylinder (8) located on the lower plate (5). An outer prismatic cylinder (11) is slidably sleeved on the outer side of the inner prismatic cylinder (10). A lower cover (3) is fixedly connected to the lower end of the outer prismatic cylinder (11). The lower cover (3) is threadedly connected to the lower end of the cylinder body (1).

2. The digestion kit for organoid and stem cell culture according to claim 1, characterized in that, Both the upper plate (4) and the lower plate (5) are disc-shaped structures. The upper plate (4) has a side hole 1 (401) evenly distributed around its circumference, and the lower plate (5) has a side hole 2 (501) opposite to the side hole 1 (401). A side rubber sleeve (20) is fixedly connected inside both the side hole 1 (401) and the side hole 2 (501). The side rubber sleeve (20) is a cylindrical structure with an I-shaped cross section. A cylindrical insertion hole 1 is opened at the center of the side rubber sleeve (20). The diameter of the insertion hole 1 is smaller than the outer diameter of the liquid storage tube (9).

3. The digestion kit for organoid and stem cell culture according to claim 1, characterized in that, The upper plate (4) has a central hole 1 (402) at its center, and the lower plate (5) has a central hole 2 (502) at its center. Both the central hole 1 (402) and the central hole 2 (502) are rotatably connected to a rotating cylinder (8). The rotating cylinder (8) is a cylindrical structure with an I-shaped cross section. It has a columnar hole at its center to accommodate the central rubber sleeve (7). The central rubber sleeve (7) has the same structure as the side rubber sleeve (20). The central rubber sleeve (7) has a second insertion hole at its center to accommodate the centrifuge tube (6). The diameter of the second insertion hole is smaller than the outer diameter of the centrifuge tube (6).

4. The digestion kit for organoid and stem cell culture according to claim 1, characterized in that, The centrifuge tube (6) includes a tube body (601), a threaded cap (602) is threaded to the upper end of the tube body (601), and a protruding ring (603) that abuts against the lower end of the threaded cap (602) is fixedly connected to the side wall of the tube body (601). The centrifuge tube (6) and the liquid storage tube (9) have the same structure.

5. A digestion kit for organoid and stem cell culture according to claim 1, characterized in that, The cylinder (1) is a hollow cylindrical structure with openings at both the top and bottom. The centrifuge tube (6), the liquid storage tube (9) and the cylinder (1) are all made of transparent material, which is one of polymethyl methacrylate, polystyrene and polycarbonate.

6. The digestion kit for organoid and stem cell culture according to claim 1, characterized in that, A pair of arc-shaped plates (12) are provided above the lower cover (3). A slider (13) is fixedly connected to the lower end of the arc-shaped plate (12). A radial groove (302) for sliding the slider (13) is provided on the upper surface of the lower cover (3). A guide rod (14) that passes through the slider (13) is fixedly connected to the inner wall of the radial groove (302). A spring (15) that abuts against the inner end of the slider (13) is sleeved on the guide rod (14). An annular groove (101) for vertical sliding of the arc-shaped plate (12) is provided on the lower part of the inner wall of the cylinder (1).

7. A digestion kit for organoid and stem cell culture according to claim 6, characterized in that, The inner prismatic tube (10) and the outer prismatic tube (11) are both cylindrical structures with a regular polygonal cross-section. The annular groove (101) is an annular groove with a rectangular cross-section, and the arc plate (12) is an arc block with an inverted L-shaped cross-section.

8. A digestion kit for organoid and stem cell culture according to claim 1, characterized in that, The upper cover (2) is slidably connected to the inner side of the squeezing cylinder (18). The lower end of the squeezing cylinder (18) abuts against the upper end of the centrifuge tube (6) and the liquid storage tube (9). The inner wall of the squeezing cylinder (18) abuts against the second spring (19), and the upper end of the second spring (19) abuts against the inner wall of the upper cover (2).

9. A digestion kit for organoid and stem cell culture according to claim 8, characterized in that, The lower end of the upper cover (2) is provided with an annular groove three (202) for the extrusion cylinder (18) to slide vertically. The upper cover (2) is provided with an annular groove two (201) on the outside of the annular groove three (202). An upper sealing ring (17) is fixedly connected in the annular groove two (201). An annular groove four (301) is provided on the lower cover (3). A lower sealing ring (16) is fixedly connected in the annular groove four (301).

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