An adjustable scratch device for cell scratch experiments

Abstract

The utility model provides a kind of adjustable scratch device for cell scratch experiment, including C type connecting sheet, the two sides of C type connecting sheet are outwardly provided with slice, C type connecting sheet makes two pieces of slice have the elasticity outward, the one end of C type connecting sheet is between two pieces of slice and is provided with rotating limiting sheet, the middle part of rotating limiting sheet is provided with rotating hole, the one end of C type connecting sheet away from rotating limiting sheet is movably provided with limiting cap, limiting cap is outwardly provided with the threaded connection sleeve with internal thread to rotating limiting sheet direction, rotating hole is rotatably connected with the threaded connection sleeve screw thread connection screw shaft, the one end of screw shaft is set with knob and extends out rotating hole, limiting cap is outwardly provided with limiting baffle to rotating limiting sheet direction between two pieces of slice, the inside of each limiting baffle gradually thins to rotating limiting sheet direction.The utility model is easy to operate, scratch precision is high and width can be adjusted according to need, to improve the accuracy and repeatability of cell scratch experiment.

Description

Technical Field

[0001] This invention belongs to the field of cell experiment technology and relates to an adjustable scratching device for cell scratching experiments. Background Technology

[0002] In cell biology and pharmacology research, the cell scratch assay (also known as the wound healing assay) is a classic in vitro model widely used to assess cell migration ability. This method simulates the repair process after tissue damage in vivo by creating a "scratch" or "wound" on a monolayer of adherent cells, allowing observation of the cells' ability to migrate to the scratched area at specific time points. This enables research into cell migration mechanisms, drug intervention effects, or gene function regulation. Traditional scratching techniques typically use sterile pipette tips, cell scrapers, or specialized scratching tools to physically scrape away cells from a monolayer in a culture dish or plate, creating a cell-free region. However, this procedure is highly dependent on the operator's manual skills and experience. In practice, uneven force, angle deviations, or inconsistent movement speeds can easily cause the scratched lines to deviate from the intended path, resulting in irregular edges and inconsistent widths under a microscope. Furthermore, existing scratching tools are mostly of fixed size, making it difficult to flexibly adjust the scratch width according to experimental needs, thus limiting the standardization and reproducibility of the experiment. Utility Model Content

[0003] The purpose of this invention is to provide an adjustable scratching device for cell scratch experiments, which is easy to operate, has high scratching accuracy, and allows for width adjustment as needed, thereby improving the accuracy and repeatability of cell scratch experiments.

[0004] To solve the above-mentioned technical problems, this utility model provides an adjustable scratching device for cell scratching experiments, including a C-shaped connecting piece. Scratch blades are arranged outwards on both sides of the C-shaped connecting piece, giving the two scratch blades outward elasticity. A rotation limiting piece is provided at one end of the C-shaped connecting piece between the two scratch blades. A rotating hole is formed in the middle of the rotating limiting piece. A limiting cap is movably provided at the end of the C-shaped connecting piece away from the rotating limiting piece. A threaded connecting sleeve with internal threads is provided on the limiting cap towards the rotating limiting piece. The threaded connecting sleeve contacts the inner wall of the C-shaped connecting piece. A threaded shaft threadedly connected to the threaded connecting sleeve is rotatably connected to the rotating hole. A knob is provided at one end of the threaded shaft extending out of the rotating hole. A limiting stop is provided on the limiting cap outside the two scratch blades towards the rotating limiting piece, with the inner side of each limiting stop gradually thinning towards the rotating limiting piece.

[0005] By employing the above technical solution, when conducting cell scratch experiments, a certain amount of culture medium is added to the culture dish. Finally, cells of the required concentration are added to both sides of scratch arm 1. The cells are then cultured until they cover the bottom of the culture well or reach 80-90% of the bottom. The two scratches are then pinched together by hand, forming a line between the two cutting edges. The two cutting edges are placed in the culture dish, and then the pinch is released. The two scratches move apart under elastic force, separating the cells on the culture dish to form scratches. If the scratch width needs to be adjusted, simply turn the knob to rotate the threaded shaft, which pushes or pulls the threaded connecting sleeve, moving the limiting stop plate and thus adjusting the distance between the free edges of the two scratches.

[0006] The present invention is further configured such that the free edge of each slitting blade is a cutting edge.

[0007] The present invention is further configured such that the outer diameter of the threaded shaft is larger than the inner diameter of the rotating hole, and an annular rotating groove that mates with the rotating hole is provided on the outer periphery of the end of the threaded shaft.

[0008] The present invention is further configured such that an indicator scale is provided on the outer side of one of the limiting baffles, the indicator scale corresponding to the gap that can be opened between the free edges of the two slitting plates.

[0009] The present invention is further configured such that each slitting plate is chamfered on the side closest to the limiting cap.

[0010] The present invention is further configured such that the C-shaped connecting piece and each slitting piece are made of stainless steel.

[0011] Compared with the prior art, the present invention has the following beneficial effects: Firstly, by setting an adjustable threaded shaft and threaded connecting sleeve structure, experimenters can precisely control the distance between the free edges of the two scribes, thereby flexibly adjusting the scribe width according to experimental needs, which greatly improves the standardization and repeatability of the experiment.

[0012] Secondly, the design of the C-shaped connecting piece gives the sliding piece outward elasticity. Combined with the setting of the rotation limit piece and the limit stop piece, it ensures that the sliding piece can be stably separated after being released, forming a neat scratch area, effectively avoiding the problem of irregular scratches caused by improper manual operation. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 Used to demonstrate the connection of C-type connecting pieces, slitting pieces, and rotation limit pieces; Figure 3Used to demonstrate the connection between the threaded sleeve and the threaded shaft.

[0014] Among them, 1. C-type connecting piece; 2. Sliding piece; 3. Cutting edge; 4. Rotation limit piece; 5. Rotating hole; 6. Limit cap; 7. Threaded connecting sleeve; 8. Threaded shaft; 9. Annular rotating groove; 10. Knob; 11. Limit stop piece; 12. Indicator scale. Detailed Implementation

[0015] The adjustable scratching device for cell scratch experiments proposed in this invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. The advantages and features of this invention will become clearer from the following description. It should be noted that the drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of this invention. The same or similar reference numerals in the drawings represent the same or similar parts.

[0016] Example, refer to Figure 1-3 An adjustable scratching device for cell scratching experiments includes a C-shaped connecting piece 1, with a scratching blade 2 on each side of the C-shaped connecting piece 1. Both the C-shaped connecting piece 1 and each scratching blade 2 are made of stainless steel. The free edge of each scratching blade 2 is a cutting edge 3. The C-shaped connecting piece 1 gives the two scratching blades 2 outward elasticity, so that when the free edges of the two scratching blades 2 are pressed inward and close together, the two cutting edges 3 can form a line.

[0017] A rotating limiting plate 4 is provided at one end of the C-type connecting piece 1 between two slitting plates 2, and a rotating hole 5 is provided in the middle of the rotating limiting plate 4. A limiting cap 6 is movably provided at the end of the C-type connecting piece 1 away from the rotating limiting plate 4. A threaded connecting sleeve 7 with internal threads is provided in the direction of the rotating limiting plate 4 from the limiting cap 6. The threaded connecting sleeve 7 contacts the inner wall of the C-type connecting piece 1. A threaded shaft 8 is rotatably connected to the rotating hole 5 and is threaded to the threaded connecting sleeve 7. The outer diameter of the threaded shaft 8 is larger than the inner diameter of the rotating hole 5. An annular rotating groove 9 that mates with the rotating hole 5 is provided on the outer circumference of the end of the threaded shaft 8. A knob 10 is provided at one end of the threaded shaft 8 that extends out of the rotating hole 5.

[0018] A limiting cap 6 has a limiting baffle 11 located on the outer side of the two sliding pieces 2 in the direction of rotational limiting piece 4. The inner side of each limiting baffle gradually thins in the direction of rotational limiting piece 4, so that the more the limiting baffle 11 moves in the direction of rotational limiting piece 4, the more the two pieces will press the two sliding pieces 2 inward, so that the gap that can be opened between the free edges of the two sliding pieces 2 gradually decreases. The side of each sliding piece 2 near the limiting cap 6 is chamfered. An indicator scale 12 is provided on the outer side of one of the limiting baffles 11, and the indicator scale 12 corresponds to the gap that can be opened between the free edges of the two sliding pieces 2.

[0019] Working principle: In the cell scratch assay, a certain amount of culture medium is added to the culture dish. Then, cells of the required concentration are added to both sides of the scratch arm 1. The cells are cultured until they cover the bottom of the culture well or reach 80-90% of the bottom. The two scratch blades 2 are then pinched together so that the two edges 3 form a line. The two edges 3 are placed in the culture dish, and then the pinch is released. The two scratch blades 2 move apart under elastic force, separating the cells on the culture dish to form scratches. To adjust the scratch width, simply turn the knob 10 to rotate the threaded shaft 8. This pushes or pulls the threaded connecting sleeve 7, causing the limiting baffle 11 to move, thus adjusting the distance between the free edges of the two scratch blades 2.

[0020] It should also be noted that all terms such as "set up" and similar descriptive words in this application (especially the specification) indicate that two structures have or exist a connection relationship. However, the specific means by which the two are connected are not limited in detail, and are usually conventional connection methods. That is, the means should be understood as prior art and do not need to be elaborated. For example, "m is set up with n" only indicates that structure m has structure n, and whether the two are connected by welding, riveting, adhesive, or integral molding is within the scope of protection of this application. Similarly, "x is rotatably set up with y" only indicates that y and x can rotate relative to each other, and whether the two are connected by a bearing, or whether y directly passes through x and is rotatably connected to x, or other feasible methods, are all within the scope of protection of this application.

[0021] The above description is only a description of the preferred embodiment of the present utility model and is not intended to limit the scope of the present utility model in any way. Any changes or modifications made by those skilled in the art based on the above disclosure shall fall within the protection scope of the claims.

Claims

1. An adjustable scratching device for cell scratch experiments, comprising a C-type connector (1), characterized in that, Both sides of the C-shaped connecting piece (1) are provided with outward-facing slats (2). The C-shaped connecting piece (1) gives the two slats (2) outward elasticity. A rotation limiting piece (4) is provided at one end of the C-shaped connecting piece (1) between the two slats (2). A rotating hole (5) is provided in the middle of the rotation limiting piece (4). A limiting cap (6) is movably provided at the end of the C-shaped connecting piece (1) away from the rotation limiting piece (4). The limiting cap (6) is provided with an inner... A threaded connecting sleeve (7) is provided, which contacts the inner wall of the C-shaped connecting piece (1). The rotating hole (5) is rotatably connected to a threaded shaft (8) that is threaded to the threaded connecting sleeve (7). One end of the threaded shaft (8) extends out of the rotating hole (5) and is provided with a knob (10). The limiting cap (6) is provided with a limiting stop (11) on the outside of the two slitting pieces (2) towards the rotating limiting piece (4). The inner side of each limiting stop gradually thins towards the rotating limiting piece (4).

2. The adjustable scratching device for cell scratch experiments according to claim 1, characterized in that, The free edge of each slicing piece (2) is the cutting edge (3).

3. The adjustable scratching device for cell scratch experiments according to claim 1, characterized in that, The outer diameter of the threaded shaft (8) is larger than the inner diameter of the rotating hole (5), and an annular rotating groove (9) that mates with the rotating hole (5) is provided on the outer periphery of the end of the threaded shaft (8).

4. The adjustable scratching device for cell scratch experiments according to claim 1, characterized in that, One of the limiting plates (11) has an indicator scale (12) on its outer side, which corresponds to the gap that can be opened between the free edges of the two slitting plates (2).

5. The adjustable scratching device for cell scratch experiments according to claim 1, characterized in that, Each slit (2) is chamfered on the side closest to the limiting cap (6).

6. An adjustable scratching device for cell scratch experiments according to any one of claims 1-5, characterized in that, The C-type connecting piece (1) and each slitting piece (2) are made of stainless steel.

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