A multi-layer filtration separation device for rapid screening of cell clusters

Abstract

The utility model provides a kind of multilayer filtration separation device for cell mass rapid screening belongs to hematopoietic stem cell screening technical field, including filtration separation mechanism, including sample cup, the collection container fixed in the bottom of sample cup, the connecting block being set in the bottom of collection container, the reserved groove being opened in the inside of collection container, the arc slide plate being slidably installed in the inside of reserved groove, several communication grooves being opened in the surface of arc slide plate;Adaptation mechanism, including the switching component for switching use to filter membrane, and the locking component used to improve the limiting effect after switching with switching component use.This utility model is switched to different aperture filter membrane by switching component, to adapt to different needs in the process of hematopoietic stem cell rapid screening, solve the aperture of traditional filter membrane fixed, the scope of application reduces, and locking component is used to improve the use stability of filter membrane after switching, ensure normal use of device.

Description

Technical Field

[0001] This invention belongs to the field of hematopoietic stem cell screening technology, specifically relating to a multilayer filtration and separation device for rapid screening of cell clusters. Background Technology

[0002] Cell cluster screening refers to the process of identifying and separating target cell clusters from a mixed cell population based on morphology, size, surface markers, or functional characteristics using specific techniques (such as flow cytometry, microscopy, etc.). This technology is widely used in stem cell research, tumor biology, and organoid construction, and is a key cutting-edge step in obtaining high-purity specific cell populations for downstream analysis and experiments.

[0003] In the field of cell cluster screening, traditional filtration and separation devices often have defects and cannot meet the needs of efficient and accurate screening. The filter membranes of existing devices are mostly fixed. When it is necessary to change the filter membrane with different pore sizes according to the characteristics of different hematopoietic stem cell samples, it is often necessary to disassemble multiple parts, which is cumbersome and time-consuming. Frequent disassembly can lead to a decrease in the sealing of the device, increase the risk of sample contamination, and may also damage the device components and reduce the service life of the device. Utility Model Content

[0004] The purpose of this invention is to provide a multilayer filtration and separation device for rapid screening of cell clusters, aiming to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: A multilayer filtration separation device for rapid screening of cell clusters, comprising, The filtration and separation mechanism includes a sample cup, a collection container fixed to the bottom of the sample cup, a connecting block set at the bottom of the collection container, a reserved groove opened inside the collection container, an arc-shaped sliding plate slidably installed inside the reserved groove, several connecting grooves opened on the surface of the arc-shaped sliding plate, filter membranes with different pore sizes respectively set inside the several connecting grooves, and an annular sealing gasket set on the surface of the arc-shaped sliding plate. The adapter mechanism includes a switching component for switching the filter membrane, and a locking component used in conjunction with the switching component to improve the limiting effect after switching.

[0006] As a preferred embodiment of this utility model, the switching component includes an arc plate fixed to the surface of the arc-shaped sliding plate, a plurality of protruding teeth fixed to the inner side of the arc plate, a rotating shaft rotatably mounted on the bottom of the collection container, and a drive gear fixed to the other end of the rotating shaft and meshing with the protruding teeth.

[0007] In a preferred embodiment of this utility model, a worm gear is fixedly mounted on the surface of the rotating shaft, and a worm meshes with the worm gear and is used to drive the worm gear to rotate.

[0008] As a preferred embodiment of this utility model, a mounting frame is fixedly installed on the surface of the collection container, the worm gear is rotatably installed inside the mounting frame, and a handwheel is provided on one side of the worm gear.

[0009] As a preferred embodiment of this utility model, the locking component includes a side frame fixed to the surface of the collection container, a connecting rod elastically mounted on the side frame, a locking ball fixed to one end of the connecting rod, and several arc-shaped grooves opened at the bottom of the drive gear and used in conjunction with the locking ball.

[0010] As a preferred embodiment of this utility model, a plurality of the arc-shaped grooves are distributed at equal angles along the circumferential direction of the drive gear, and the surface of the retaining ball is engaged with the interior of the arc-shaped groove.

[0011] In a preferred embodiment of this utility model, the connecting rod passes through the side frame, and a tension spring is fixedly installed at the bottom of the side frame. The other end of the tension spring is fixedly connected to the end of the connecting rod, and the tension spring is used to maintain the compressive force on the connecting rod.

[0012] Compared with the prior art, the beneficial effects of this utility model are: by using a switching component to switch between filter membranes with different pore sizes to adapt to the different needs in the rapid screening process of hematopoietic stem cells, the problem of fixed pore size of traditional filter membranes and reduced applicability is solved. At the same time, the locking component is used to improve the stability of the filter membrane after switching and ensure the normal use of the device. Attached Figure Description

[0013] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them: Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the adapter mechanism structure of this utility model; Figure 3 This is a schematic diagram of the switching component structure of this utility model; Figure 4 This is a schematic diagram of the locking component structure of this utility model.

[0014] In the diagram: 100, filtration and separation mechanism; 110, sample cup; 120, collection container; 130, connecting block; 140, arc-shaped sliding plate; 150, filter membrane; 160, annular sealing gasket; 200, adapter mechanism; 210, switching component; 211, arc plate; 212, convex tooth; 213, rotating shaft; 214, drive gear; 215, worm gear; 216, worm; 217, mounting bracket; 220, locking component; 221, side frame; 222, connecting rod; 223, retaining ball; 224, arc groove; 225, tension spring. Detailed Implementation

[0015] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0016] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0017] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0018] Example Reference Figure 1-4 This embodiment of the present invention provides a multilayer filtration separation device for rapid screening of cell clusters, comprising: The filtration and separation mechanism 100 includes a sample cup 110, a collection container 120 fixed to the bottom of the sample cup 110, a connecting block 130 disposed at the bottom of the collection container 120, a reserved groove opened inside the collection container 120, an arc-shaped sliding plate 140 slidably installed inside the reserved groove, a plurality of connecting grooves opened on the surface of the arc-shaped sliding plate 140, filter membranes 150 respectively disposed inside the plurality of connecting grooves and having different pore sizes, and an annular sealing gasket 160 disposed on the surface of the arc-shaped sliding plate 140. The adapter mechanism 200 includes a switching component 210 for switching the filter membrane 150, and a locking component 220 used in conjunction with the switching component 210 to improve the limiting effect after switching.

[0019] The switching component 210 is used to switch the filter membrane 150 with different pore sizes to adapt to the different needs in the rapid screening process of hematopoietic stem cells. This solves the problem of the fixed pore size of the traditional filter membrane 150, which reduces its applicable range. At the same time, the locking component 220 is used to improve the stability of the filter membrane 150 after switching and ensure the normal use of the device.

[0020] Specifically, the switching component 210 includes an arc plate 211 fixed to the surface of the arc-shaped slide plate 140, a plurality of protruding teeth 212 fixed to the inner side of the arc plate 211, a rotating shaft 213 rotatably mounted on the bottom of the collection container 120, and a drive gear 214 fixed to the other end of the rotating shaft 213 and meshing with the protruding teeth 212.

[0021] When the drive gear 214 rotates under the drive of the rotating shaft 213, it can drive the arc plate 211 to move through gear meshing transmission, thereby driving the arc-shaped slide plate 140 to slide in the reserved groove, realizing the switching of filter membranes 150 with different pore sizes.

[0022] Furthermore, a worm gear 215 is fixedly mounted on the surface of the rotating shaft 213, and a worm 216 meshes with the worm gear 215 and is used to drive the worm gear 215 to rotate.

[0023] In this process, the worm gear 216 drives the worm wheel 215 to rotate, which in turn drives the rotating shaft 213 and the drive gear 214 to rotate to achieve filter membrane switching. The worm gear structure can prevent the worm wheel 215 from rotating on its own without external force, thereby avoiding positional displacement of the rotating shaft 213, drive gear 214 and arc-shaped slide plate 140. This ensures that the filter membrane 150 in the working position can remain stable and will not affect the accuracy of hematopoietic stem cell screening due to device vibration or other external factors.

[0024] Preferably, a mounting bracket 217 is fixedly mounted on the surface of the collection container 120, and a worm gear 216 is rotatably mounted inside the mounting bracket 217. A handwheel is provided on one side of the worm gear 216.

[0025] The mounting bracket 217 provides a stable mounting support structure for the worm gear 216, ensuring that the worm gear 216 can maintain a stable axial position during rotation, and avoiding poor meshing between the worm gear 216 and the worm wheel 215 due to unstable installation, which would affect the transmission effect and filter membrane switching accuracy.

[0026] Furthermore, the locking component 220 includes a side frame 221 fixed to the surface of the collection container 120, a connecting rod 222 elastically mounted on the side frame 221, a locking ball 223 fixed to one end of the connecting rod 222, and a plurality of arc-shaped grooves 224 opened at the bottom of the drive gear 214 and used in conjunction with the locking ball 223.

[0027] The connecting rod 222, which is elastically mounted on the side frame 221, can drive the locking ball 223 to move under elastic action. When the filter membrane is switched to the target position, the locking ball 223 can be locked into the arc groove 224 corresponding to the bottom of the drive gear 214. Through the locking cooperation between the locking ball 223 and the arc groove 224, the drive gear 214 is limited to prevent the drive gear 214 from rotating during the screening process, thereby avoiding the positional displacement between the arc-shaped slide plate 140 and the filter membrane 150.

[0028] Specifically, several arc-shaped grooves 224 are distributed at equal angles along the circumferential direction of the drive gear 214, and the surface of the ball 223 is engaged with the inside of the arc-shaped grooves 224.

[0029] Furthermore, the connecting rod 222 passes through the side frame 221, and a tension spring 225 is fixedly installed at the bottom of the side frame 221. The other end face of the tension spring 225 is fixedly connected to the end of the connecting rod 222. The tension spring 225 is used to maintain the compressive force on the connecting rod 222.

[0030] The tension spring 225 continuously applies pressure to the connecting rod 222, keeping the locking ball 223 in contact with the bottom of the drive gear 214. When the drive gear 214 rotates to the target position and the arc groove 224 aligns with the locking ball 223, the elastic force of the tension spring 225 can push the locking ball 223 to quickly lock into the arc groove 224, achieving automatic locking without the need for additional manual operation, thus improving the automation and convenience of the locking operation.

[0031] In use, the operator turns the handwheel, which drives the worm 216 to rotate inside the mounting bracket 217. Since the worm 216 meshes with the worm wheel 215 on the surface of the rotating shaft 213, the rotation of the worm 216 drives the worm wheel 215 to rotate, which in turn drives the rotating shaft 213 to rotate with the drive gear 214 fixed at the end of the rotating shaft 213. The drive gear 214 meshes with the convex teeth 212 on the inner side of the arc plate 211. The rotation of the drive gear 214 drives the arc plate 211 to move. The arc plate 211 drives the arc-shaped sliding plate 140 fixedly connected to it to slide in the reserved groove inside the collection container 120 until the filter membrane 150 of the required pore size moves to the working position that communicates with the sample cup 110 and the collection container 120. During the process of the arc-shaped slide plate 140 driving the filter membrane 150 to the target position, the drive gear 214 rotates synchronously. When the filter membrane 150 reaches the working position, a certain arc groove 224 at the bottom of the drive gear 214 is just aligned with the locking ball 223 in the locking component 220. At this time, the elastic force of the tension spring 225 at the bottom of the side frame 221 acts on the connecting rod 222, pushing the connecting rod 222 to move along the side frame 221, so that the locking ball 223 is engaged in the corresponding arc groove 224, thereby locking the drive gear 214, and thus fixing the position of the arc-shaped slide plate 140 and the filter membrane 150, completing the filter membrane switching and locking.

[0032] In summary, the switching component 210 is used to switch between filter membranes 150 with different pore sizes to meet the different needs in the rapid screening process of hematopoietic stem cells. This solves the problem of the fixed pore size of the traditional filter membrane 150, which reduces its applicability. At the same time, the locking component 220 is used to improve the stability of the filter membrane 150 after switching and ensure the normal use of the device.

[0033] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape and proportion of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of this utility model. The order or sequence of any process or method steps may be changed or rearranged according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structural equivalents but also equivalent structures. Without departing from the scope of this invention, other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments. Therefore, this invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0034] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the present invention as currently considered, or those features that are not relevant to implementing the present invention) may be omitted.

[0035] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0036] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A multilayer filtration separation device for rapid screening of cell clusters, characterized in that: include, The filtration and separation mechanism (100) includes a sample cup (110), a collection container (120) fixed to the bottom of the sample cup (110), a connecting block (130) set at the bottom of the collection container (120), a reserved groove opened inside the collection container (120), an arc-shaped slide plate (140) slidably installed inside the reserved groove, a plurality of connecting grooves opened on the surface of the arc-shaped slide plate (140), filter membranes (150) respectively set inside the plurality of connecting grooves and having different pore sizes, and an annular sealing gasket (160) set on the surface of the arc-shaped slide plate (140). The adapter (200) includes a switching component (210) for switching the filter membrane (150) and a locking component (220) used in conjunction with the switching component (210) to improve the limiting effect after switching.

2. The multilayer filtration separation device for rapid screening of cell clusters according to claim 1, characterized in that: The switching component (210) includes an arc plate (211) fixed to the surface of the arc-shaped slide plate (140), a plurality of protruding teeth (212) fixed to the inner side of the arc plate (211), a rotating shaft (213) rotatably mounted on the bottom of the collection container (120), and a drive gear (214) fixed to the other end of the rotating shaft (213) and meshing with the protruding teeth (212).

3. The multilayer filtration separation device for rapid screening of cell clusters according to claim 2, characterized in that: A worm gear (215) is fixedly mounted on the surface of the shaft (213), and a worm (216) meshes with the worm gear (215) and is used to drive the worm gear (215) to rotate.

4. The multilayer filtration separation device for rapid screening of cell clusters according to claim 3, characterized in that: The surface of the collection container (120) is fixedly mounted with a mounting bracket (217), and the worm gear (216) is rotatably mounted inside the mounting bracket (217). A handwheel is provided on one side of the worm gear (216).

5. A multilayer filtration separation device for rapid screening of cell clusters according to claim 4, characterized in that: The locking component (220) includes a side frame (221) fixed to the surface of the collection container (120), a connecting rod (222) elastically mounted on the side frame (221), a locking ball (223) fixed to one end of the connecting rod (222), and a number of arc grooves (224) opened at the bottom of the drive gear (214) and used in conjunction with the locking ball (223).

6. A multilayer filtration separation device for rapid screening of cell clusters according to claim 5, characterized in that: Several of the arc-shaped grooves (224) are distributed at equal angles along the circumferential direction of the drive gear (214), and the surface of the retaining ball (223) is engaged with the interior of the arc-shaped groove (224).

7. A multilayer filtration separation device for rapid screening of cell clusters according to claim 6, characterized in that: The connecting rod (222) passes through the side frame (221). A tension spring (225) is fixedly installed at the bottom of the side frame (221). The other end face of the tension spring (225) is fixedly connected to the end of the connecting rod (222). The tension spring (225) is used to maintain the compressive force on the connecting rod (222).

Images