Multilayer filtration centrifuge device for exosome separation

Abstract

The utility model relates to biological separation equipment technical field, concretely is a kind of multilayer filtering centrifugal device for exosome separation, including main part, lid, rotator, communicating pipe, collecting pipe and collecting device, the lid is installed on the upper surface of main part, the rotator is installed in the inside of main part, the communicating pipe is installed in the inside of rotator, the collecting pipe is installed in the lower end of communicating pipe, the collecting device is installed on the surface of collecting pipe, the collecting device includes first connecting block and second connecting block, the first connecting block is fixedly connected in the side of collecting pipe, the second connecting block is fixedly connected in the side of communicating pipe, the lower end of communicating pipe is equipped with installation groove, the collecting pipe is inserted and is connected in the inside of installation groove, the inside fixedly connected with filter plate of communicating pipe, the utility model collecting device, the filtration resistance when realizing to liquid centrifugation, it is convenient to carry out quick filtration collection to the exosome in liquid, improves the collection efficiency of equipment.

Description

Technical Field

[0001] This utility model relates to the field of biological separation equipment technology, and in particular to a multi-layer filtration centrifuge device for exosome separation. Background Technology

[0002] Exosomes are nanoscale vesicles with a diameter of 30-150 nm secreted by cells. Their core function is to act as carriers of intercellular information, carrying bioactive substances such as proteins, nucleic acids, and lipids, and participating in physiological and pathological processes such as immune regulation, tissue repair, and signal transduction. They are widely present in bodily fluids such as blood, urine, and saliva. Exosomes have great potential applications in disease diagnosis (such as early cancer screening), regenerative medicine, and targeted therapy, and are currently a research hotspot in the fields of biomedicine and biotechnology, providing new directions for disease treatment and health management. When separating exosomes, a multilayer filtration centrifuge device for exosome separation is used to rapidly separate exosomes from cells.

[0003] However, the existing equipment has the following shortcomings: When centrifuging liquids, the existing equipment generally uses test tubes to centrifuge the liquids arbitrarily. After centrifugation, the liquids are not clearly separated into layers, making it difficult to quickly collect exosomes, resulting in low equipment collection efficiency.

[0004] Therefore, we propose a multilayer filtration centrifugation device for exosome separation to solve the problems mentioned above. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies where equipment struggles to quickly separate and extract exosomes after centrifuging liquids, resulting in low efficiency for users in collecting exosomes. Therefore, this invention proposes a multi-layer filtration centrifugation device for exosome separation.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a multi-layer filtration centrifuge device for exosome separation, comprising a main body, a cover, a rotator, a connecting tube, a collecting tube, and a collecting device. The cover is installed on the upper surface of the main body, the rotator is installed inside the main body, the connecting tube is installed inside the rotator, the collecting tube is installed at the lower end of the connecting tube, and the collecting device is installed on the surface of the collecting tube. The collecting device includes a first connecting block and a second connecting block. The first connecting block is fixedly connected to the side of the collecting tube, and the second connecting block is fixedly connected to the side of the connecting tube. An installation groove is formed at the lower end of the connecting tube, and the collecting tube is inserted into the installation groove. A filter plate is fixedly connected inside the connecting tube. A guide rod is fixedly connected to the surface of the first connecting block, and a positioning rod is fixedly connected to the end of the second connecting block away from the connecting tube. A guide groove is formed on the surface of the guide rod, and a connecting plate is slidably connected to the surface of the guide rod. The connecting plate is slidably connected inside the guide groove, and the end of the connecting plate away from the guide rod is inserted into the surface of the positioning rod.

[0007] Furthermore, a fixed block is fixedly connected to the end of the guide rod away from the first connecting block. By setting the guide rod, the connecting plate can be moved horizontally, reducing the situation where the connecting plate is difficult to move smoothly during use.

[0008] Furthermore, a first spring is sleeved and connected to the surface of the guide rod.

[0009] Furthermore, one end of the first spring is fixedly connected to the surface of the connecting plate, and the end of the first spring away from the connecting plate is fixedly connected to the surface of the fixing block. The first spring is provided to facilitate the application of a restoring force to the connecting plate.

[0010] Furthermore, an auxiliary component is fixedly provided on the surface of the rotator. The auxiliary component includes a pull hole and a stop plate. The pull hole is opened on the surface of the rotator, and the stop plate is slidably connected inside the pull hole. By setting the stop plate, the connecting pipe can be limited, reducing the possibility that the connecting pipe may easily detach from the inside of the rotator and fall into the main body during use, causing liquid leakage in the connecting pipe.

[0011] Furthermore, a second spring is fixedly connected to one end of the abutment plate, and the end of the second spring away from the abutment plate is fixedly connected to the inside of the pull hole. The second spring is provided to facilitate the application of a restoring elastic force to the abutment plate.

[0012] Furthermore, a pull rod is fixedly connected to the upper surface of the abutment near the second spring. The pull rod is slidably connected inside the pull hole, and the pull rod is provided to facilitate the movement of the abutment.

[0013] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0014] 1. In this utility model, by setting up a collection device, the liquid in the connecting tube is effectively and quickly separated, which plays a convenient role in separating liquid exosomes. This reduces the problem of existing equipment using test tubes to centrifuge liquids, which often results in unclear liquid stratification after centrifugation, making it difficult to quickly collect exosomes and causing low equipment collection efficiency. This collection device achieves filtration and blocking during liquid centrifugation, facilitating the rapid filtration and collection of exosomes in the liquid, and improving the equipment's collection efficiency.

[0015] 2. In this utility model, by setting an auxiliary component, the connecting pipe is effectively limited, which plays a role in blocking the connecting pipe. This reduces the situation where the connecting pipe tends to rebound due to inertia after centrifugation, causing it to detach from the rotor and fall into the main body. This auxiliary component achieves the limitation of the connecting pipe and improves the stability of the equipment. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0017] Figure 1 This utility model provides a three-dimensional structural diagram of a multi-layer filtration centrifuge device for exosome separation;

[0018] Figure 2 This invention provides a schematic diagram of the internal structure of a multilayer filtration centrifuge device for exosome separation;

[0019] Figure 3 This utility model provides a schematic diagram of the rotor structure of a multilayer filtration centrifuge device for exosome separation;

[0020] Figure 4 This invention provides a multi-layer filtration centrifuge device for exosome separation. Figure 3 Enlarged structural diagram at point A in the middle;

[0021] Figure 5 This invention provides a multi-layer filtration centrifuge device for exosome separation. Figure 3 Enlarged structural diagram at point B;

[0022] Figure 6 This invention provides a schematic diagram of the collection tube structure of a multi-layer filtration centrifuge device for exosome separation.

[0023] Legend: 1. Main body; 2. Cover; 3. Rotator; 4. Connecting pipe; 5. Collection pipe; 6. Collection device; 61. First connecting block; 62. Second connecting block; 63. Connecting plate; 64. Positioning rod; 65. Guide rod; 66. Fixing block; 67. First spring; 68. Guide groove; 69. Filter plate; 610. Mounting groove; 7. Auxiliary component; 71. Pull hole; 72. Support plate; 73. Pull rod; 74. Second spring. Detailed Implementation

[0024] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0025] Please see Figures 1-6 This utility model provides a technical solution: a multi-layer filtration centrifuge device for exosome separation, comprising a main body 1, a cover 2, a rotor 3, a connecting tube 4, a collecting tube 5, and a collecting device 6. The cover 2 is installed on the upper surface of the main body 1, the rotor 3 is installed inside the main body 1, the connecting tube 4 is installed inside the rotor 3, and the collecting tube 5 is installed at the lower end of the connecting tube 4.

[0026] The main body 1 is the core support and drive unit of the device, integrating a high-speed centrifugal drive mechanism that operates stably and with precise speed, providing sufficient centrifugal force for exosome separation. The lid 2 is the sealing and protective component of the main body 1, featuring a leak-proof sealing design to prevent liquid splashing during centrifugation and ensure a clean experimental environment. The rotor 3 is the actuator for centrifugal motion, connected to the drive mechanism of the main body 1, driving the connecting tube 4 and the collecting tube 5 to rotate synchronously at high speed. The connecting tube 4 is a liquid transfer and filtration channel, integrating a multi-layer filtration structure to achieve graded filtration and separation of exosomes. The collecting tube 5 is the collection container for exosomes, made of sterile transparent material for easy observation of the collected amount and purity. The collecting device 6 enables a quick and stable connection between the connecting tube 4 and the collecting tube 5, and works in conjunction with the filter plate 69 to complete graded filtration, improving separation efficiency and purity.

[0027] The following section will describe the specific setup and function of its collection device 6 and auxiliary components 7.

[0028] In this implementation scheme: the collecting device 6 is installed on the surface of the collecting pipe 5. The collecting device 6 includes a first connecting block 61 and a second connecting block 62. The first connecting block 61 is the connecting carrier of the collecting pipe 5, which is firmly connected to the collecting pipe 5 and provides an installation base for the guide rod 65. The second connecting block 62 is the connecting carrier of the connecting pipe 4, which is fixed to the connecting pipe 4 and provides support for the positioning rod 64. The two work together to achieve precise docking between the collecting pipe 5 and the connecting pipe 4. The first connecting block 61 is fixedly connected to the side of the collecting pipe 5, and the second connecting block 62 is fixedly connected to the side of the connecting pipe 4. The lower end of the connecting pipe 4 has an installation groove 610, which is adapted to the upper structure of the collecting pipe 5 to ensure a sealed insertion. The manifold 5 is inserted into the installation groove 610. After insertion, it fits tightly without any liquid leakage. The filter plate 69 is fixedly connected inside the connecting pipe 4. The filter plate 69 adopts a multi-layer gradient filtration structure. The filter pore size gradually decreases from the top to the bottom, which can intercept impurities in stages and accurately separate exosomes. The guide rod 65 is fixedly connected to the surface of the first connecting block 61. The positioning rod 64 is fixedly connected to the end of the second connecting block 62 away from the connecting pipe 4. The guide groove 68 is opened on the surface of the guide rod 65. The connecting plate 63 is slidably connected to the surface of the guide rod 65. The connecting plate 63 is slidably connected inside the guide groove 68. The end of the connecting plate 63 away from the guide rod 65 is inserted and connected to the surface of the positioning rod 64.

[0029] Specifically, a fixing block 66 is fixedly connected to the end of the guide rod 65 away from the first connecting block 61. The fixing block 66 can prevent the connecting plate 63 from detaching from the guide rod 65 and ensure structural integrity.

[0030] In this embodiment, by setting the guide rod 65, the connecting plate 63 can be moved horizontally, reducing the difficulty of the connecting plate 63 moving smoothly during use.

[0031] Specifically, a first spring 67 is sleeved and connected to the surface of the guide rod 65. The first spring 67 is a compression spring made of high-elasticity stainless steel, which has long-lasting elasticity and can provide stable reset force.

[0032] Specifically, one end of the first spring 67 is fixedly connected to the surface of the connecting plate 63, and the other end of the first spring 67 away from the connecting plate 63 is fixedly connected to the surface of the fixing block 66. The first spring 67 is provided to facilitate the application of a restoring force to the connecting plate 63.

[0033] In this embodiment: An auxiliary component 7 is fixedly provided on the surface of the rotator 3. The auxiliary component 7 includes a pull hole 71 and a stop plate 72. The pull hole 71 is opened on the surface of the rotator 3, and the stop plate 72 is slidably connected inside the pull hole 71. The pull hole 71 is the installation channel for the stop plate 72, the pull rod 73 and the second spring 74. It is opened on the surface of the rotator 3 and has a regular structure. The stop plate 72 is a limiting component of the connecting pipe 4. It can tightly abut against the side of the connecting pipe 4 to prevent displacement or falling off during centrifugation.

[0034] In this embodiment: by setting the stop plate 72, the connecting pipe 4 can be limited, which reduces the possibility that the connecting pipe 4 may easily detach from the inside of the rotor 3 and fall into the inside of the main body 1 during use, causing liquid leakage in the connecting pipe 4.

[0035] Specifically, a second spring 74 is fixedly connected to one end of the abutment plate 72. The end of the second spring 74 away from the abutment plate 72 is fixedly connected to the inside of the pull hole 71. The second spring 74 is provided to facilitate the application of a restoring elastic force to the abutment plate 72.

[0036] Specifically, a pull rod 73 is fixedly connected to the upper surface of the abutment plate 72 near the second spring 74. The pull rod 73 is slidably connected inside the pull hole 71 and extends to the outside of the rotator 3. The surface is provided with anti-slip texture to facilitate manual gripping and force application. The pull rod 73 is provided to facilitate the movement of the abutment plate 72.

[0037] Working Principle: When using the equipment, the user first connects the lower end of the collecting pipe 5 to the connecting pipe 4. The user then pulls the connecting plate 63 to move it on the surface of the guide rod 65. As the connecting plate 63 moves, it abuts against the first spring 67, compressing it and generating elastic force. The collecting pipe 5 is then inserted into the mounting groove 610 at the lower end of the connecting pipe 4. The grooved end of the collecting pipe 5 is inserted into the mounting groove 610 at the lower end of the connecting pipe 4. The mounting groove 610 has a structure that fits the upper end of the collecting pipe. After a tight connection, the connecting plate 63 is released, and the first spring 67 returns to its original position, causing the connecting plate 63 to be inserted onto the surface of the positioning rod 64, mechanically locking the collecting pipe 5. Then, the liquid tank to be centrifuged is inserted into the connecting pipe 4. The cap then engages with the connecting pipe 4, and the guide rod on the side of the connecting pipe 4 is then... After the slide rail on the upper surface of the rotor 3 is placed, the cover 2 is tightly closed to the upper end of the main body 1. The main body 1 is then started to drive the internal rotor 3 to rotate and centrifuge the connecting pipe 4 and the collecting pipe 5. The liquid passes through the three-layer filter plate 69 inside the connecting pipe 4 to filter and separate the exosomes in the liquid. By setting up the collecting device 6, the liquid in the connecting pipe 4 is effectively and quickly separated, which plays a convenient role in separating liquid exosomes. This reduces the problem of existing equipment using test tubes to centrifuge liquids, which often results in unclear liquid stratification after centrifugation and difficulty in quickly collecting exosomes, leading to low equipment collection efficiency. This collecting device 6 achieves filtration and blocking during liquid centrifugation, facilitating the rapid filtration and collection of exosomes in the liquid and improving the equipment's collection efficiency.

[0038] By setting the auxiliary component 7, when the connecting pipe 4 is installed in the rotator 3, the user pulls the lever 73 to drive the abutment 72 to compress the second spring 74, generating elastic force. Then, the abutment 72 moves and places the connecting pipe 4 into the slide rail on the surface of the rotator 3. After releasing the restraint of the lever 73, the second spring 74 returns to its original position and abuts the abutment 72 to block the slide rail. By setting the auxiliary component 7, the connecting pipe 4 is effectively limited, which plays the role of blocking the connecting pipe 4. This reduces the possibility that the connecting pipe 4 will easily rebound due to inertia after centrifugation, causing it to detach from the rotator 3 and fall into the main body 1. This auxiliary component 7 achieves the limitation of the connecting pipe 4 and improves the stability of the equipment.

[0039] The above description discloses only one or more preferred embodiments of the present utility model and should not be construed as limiting the scope of the present utility model. Those skilled in the art can understand that implementing all or part of the above embodiments and making equivalent changes in accordance with the claims of the present utility model still fall within the scope of the present utility model.

Claims

1. A multilayer filtration centrifuge device for exosome separation, comprising a main body (1), a lid (2), a rotator (3), a connecting tube (4), a collecting tube (5), and a collecting device (6), characterized in that: The cover (2) is installed on the upper surface of the main body (1), the rotator (3) is installed inside the main body (1), the connecting pipe (4) is installed inside the rotator (3), and the collecting pipe (5) is installed at the lower end of the connecting pipe (4). The collecting device (6) is installed on the surface of the collecting pipe (5). The collecting device (6) includes a first connecting block (61) and a second connecting block (62). The first connecting block (61) is fixedly connected to the side of the collecting pipe (5), and the second connecting block (62) is fixedly connected to the side of the connecting pipe (4). The lower end of the connecting pipe (4) is provided with an installation groove (610). The collecting pipe (5) is inserted into the installation groove (610). A filter plate (6) is fixedly connected inside the connecting pipe (4). 9) A guide rod (65) is fixedly connected to the surface of the first connecting block (61), and a positioning rod (64) is fixedly connected to the end of the second connecting block (62) away from the connecting pipe (4). A guide groove (68) is opened on the surface of the guide rod (65), and a connecting plate (63) is slidably connected to the surface of the guide rod (65). The connecting plate (63) is slidably connected inside the guide groove (68), and the end of the connecting plate (63) away from the guide rod (65) is inserted and connected to the surface of the positioning rod (64).

2. The multilayer filtration centrifuge device for exosome separation according to claim 1, characterized in that: The guide rod (65) is fixedly connected to a fixing block (66) at the end away from the first connecting block (61).

3. The multilayer filtration centrifuge device for exosome separation according to claim 2, characterized in that: A first spring (67) is sleeved and connected to the surface of the guide rod (65).

4. The multilayer filtration centrifuge device for exosome separation according to claim 3, characterized in that: One end of the first spring (67) is fixedly connected to the surface of the connecting plate (63), and the other end of the first spring (67) away from the connecting plate (63) is fixedly connected to the surface of the fixing block (66).

5. The multilayer filtration centrifuge device for exosome separation according to claim 1, characterized in that: An auxiliary component (7) is fixedly provided on the surface of the rotator (3). The auxiliary component (7) includes a pull hole (71) and a stop plate (72). The pull hole (71) is opened on the surface of the rotator (3), and the stop plate (72) is slidably connected inside the pull hole (71).

6. The multilayer filtration centrifuge device for exosome separation according to claim 5, characterized in that: One end of the abutment (72) is fixedly connected to a second spring (74), and the end of the second spring (74) away from the abutment (72) is fixedly connected to the inside of the pull hole (71).

7. The multilayer filtration centrifuge device for exosome separation according to claim 6, characterized in that: A pull rod (73) is fixedly connected to the upper surface of the abutment plate (72) near the second spring (74), and the pull rod (73) is slidably connected inside the pull hole (71).

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