A processing device for automatic lysis of peripheral red blood cells

Abstract

The utility model discloses a kind of processing equipment for peripheral red blood cell automatic lysis, it is related to red blood cell processing technical field, including closed flexible circuit, pre-packaged solid-state lysis solution, shakeable object table, driving mechanism and control unit. Flexible circuit is made of medical flexible material, present ∞-shaped flow path path, and is equipped with integrated interface of integrated liquid injection port and outlet valve;Solid-state lysis solution is fixed in flexible circuit inner wall. Shakeable object table is fixed flexible circuit by limiting buckle, and by driving mechanism driving carries out compound motion, promote the blood in flexible circuit and lysis solution fully mix, realize red blood cell efficient uniform lysis. After lysis is completed, control unit controls object table to rotate to inverted position, facilitate sample discharge. The device realizes the automation, standardization of red blood cell lysis process, replaces manual operation, improves processing efficiency and repeatability, avoids target cell damage.

Description

Technical Field

[0001] This utility model relates to the field of red blood cell processing technology, specifically a processing device for the automatic lysis of peripheral blood red blood cells. Background Technology

[0002] In biomedical laboratories, isolating and extracting peripheral blood mononuclear cells from peripheral blood is a common pretreatment step. This process typically requires lysing the blood with a lysis buffer to remove red blood cells. In existing techniques, when lysing peripheral blood samples collected in vacuum blood collection tubes or blood bags, the red blood cells naturally settle and aggregate during storage and transportation. Therefore, after adding the lysis buffer, repeated and vigorous manual inversion and mixing are necessary to ensure sufficient contact between the lysis buffer and the red blood cells.

[0003] However, this manual mixing method is inefficient, has poor batch-to-batch repeatability, and is prone to causing excessively high local concentrations of the lysis buffer due to uneven mixing intensity and frequency, leading to damage or death of the target mononuclear cells and directly affecting the accuracy and reliability of subsequent experiments. Therefore, this invention proposes an automated lysis device for peripheral blood erythrocytes. Utility Model Content

[0004] The purpose of this invention is to provide a processing device for the automatic lysis of peripheral blood erythrocytes, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a processing device for automatic lysis of peripheral blood erythrocytes, comprising: a closed flexible circuit made of medical flexible material, configured to include at least one ∞-shaped flow path, and an integrated interface integrating an injection port for injecting blood samples and an outlet valve for discharging lysed samples; a pre-packaged solid lysis solution fixed to the inner wall of the ∞-shaped flow path of the flexible circuit; a rocking platform with a limiting buckle connected to the flexible circuit for fixing and supporting the flexible circuit to maintain the ∞-shaped flow path; a drive mechanism for driving the rocking platform to perform compound motion to cause the liquid in the flexible circuit to circulate in the ∞-shaped flow path; and a control unit electrically connected to the drive mechanism for controlling the parameters of the compound motion and controlling the rocking platform to rotate to an inverted position after lysis.

[0006] Preferably, the drive mechanism includes a stepper motor, a crank driven by the stepper motor, and a connecting rod connected to the crank; a housing is provided below the rocking shelf, and the rocking shelf is connected to a support plate inside the housing via a ball joint, the ball head of the ball joint is fixed to the top of the support plate, and its cup is fixed to the center of the bottom of the rocking shelf via a connecting post; a column is hinged at a position off-center at the bottom of the rocking shelf, and the end of the connecting rod away from the crank is rotatably connected to the column.

[0007] Preferably, the flexible circuit is an annular soft bag that is integrally formed and has a flat elliptical cross-section inside.

[0008] Preferably, the column and connecting rod form an L-shaped structure.

[0009] Preferably, in the horizontal plane, the length of the crank is equal to the distance from the column to the center of the rocking platform; the length of the connecting rod is equal to the distance from the center of the rocking platform to the stepper motor drive shaft; and the length of the connecting rod is greater than the length of the crank.

[0010] Preferably, the support plate has an opening to accommodate the range of motion of the column.

[0011] Preferably, a wedge-shaped block is fixedly provided at the bottom of the housing, and a stop block is provided at the bottom of the wedge-shaped block; the lower side of the wedge-shaped block is rotatably connected to the base via a rotating shaft; a linear guide rail is fixedly provided at the bottom inner side of the base, and the linear guide rail is driven by a control unit, and the stop block is fixed to the slider of the linear guide rail; the linear guide rail drives the stop block to move towards the wedge-shaped block, squeezing the inclined surface at the bottom of the wedge-shaped block, thereby driving the wedge-shaped block and the entire housing to rotate together until the rocking platform is rotated to a vertical state, so that the integrated interface is located below the flexible circuit.

[0012] Preferably, the pre-encapsulated solid lysis solution is an ammonium salt, which is fixed to the inner wall of the flexible circuit in tablet form.

[0013] Preferably, the limiting buckle includes a pressure block, and a screw is provided on the outer side of both ends of the flexible circuit. The screw is fixed to the top of the rocking platform. Each screw has two nuts threaded on it. One end of the pressure block is sleeved with the screw, and the other end is pressed on the outer side of the flexible circuit. The pressure block is located between the two nuts. By adjusting the height of the two nuts, the two pressure blocks are used to limit and fix the flexible circuit.

[0014] Preferably, the flexible circuit has symmetrical protrusions fixed on the outer sides at both ends, and the bottom of the pressure block has a groove that matches the protrusions.

[0015] Preferably, spring members are distributed circumferentially on the outer side of the connecting post between the ball cup of the ball hinge and the rocking shelf. One end of the spring member is fixed to the bracket at the top of the support plate, and the other end abuts against the connecting post between the ball cup of the ball hinge and the rocking shelf.

[0016] Compared with traditional technologies, the beneficial effects of this utility model are:

[0017] This device employs an automated structure combining a closed, flexible circuit with a rocking platform, along with a composite motion controlled by a drive mechanism, to achieve efficient and uniform mixing of blood samples and pre-packaged solid lysis buffer in a zigzag flow path. This design replaces the traditional manual operation mode that relies on repeated and violent inverting and mixing, not only improving lysis efficiency but also ensuring high consistency and repeatability of the processing across different batches through standardized mechanically controlled motion. This effectively avoids the problem of excessively high local concentrations of lysis buffer caused by uneven manual operation force and frequency.

[0018] This device combines the ∞-shaped flow path of the flexible loop with the combined motion of the rocking stage to generate a gentle but sufficient circulating flow within the flexible loop, ensuring uniform contact between the lysate and the red blood cells. At the same time, it avoids excessive shear stress in terms of mechanical force control, thereby minimizing the damage and death of the target mononuclear cells during the lysis process and ensuring the accuracy and reliability of subsequent experiments.

[0019] This device integrates the injection port and outlet valve into a single integrated interface, and automatically rotates the rocking stage to an inverted position via the control unit after lysis, facilitating the smooth discharge of the lysed sample. This achieves full automation from sample loading and lysis to collection. Furthermore, the pre-packaged solid lysis buffer eliminates the need for users to prepare and add lysis buffer themselves, reducing operational errors and lowering the skill requirements for operators and potential biosafety risks. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the housing of this utility model;

[0022] Figure 3 This is a schematic diagram of the drive mechanism structure of this utility model;

[0023] Figure 4 This is a schematic diagram of the support plate part of this utility model;

[0024] Figure 5 This is a schematic diagram showing the position of the crank and connecting rod associated structure in the horizontal plane of this utility model;

[0025] Figure 6 This is a schematic diagram of the cross-section of the flexible circuit of this utility model;

[0026] Figure 7 This is a schematic diagram of the wedge-shaped block part of the present invention;

[0027] Figure 8This is a schematic diagram of the pressing block part of this utility model.

[0028] In the diagram: 1. Flexible circuit; 2. Integrated interface; 3. Pre-packaged solid pyrolysis fluid; 4. Rocking platform; 5. Limiting buckle; 6. Drive mechanism; 7. Control unit; 8. Stepper motor; 9. Crank; 10. Connecting rod; 11. Housing; 12. Support plate; 13. Ball joint; 14. Column; 15. Opening; 16. Wedge block; 17. Abutment block; 18. Base; 19. Linear guide rail; 20. Pressure block; 21. Screw; 22. Nut; 23. Protrusion; 24. Sink; 25. Spring. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] Example 1:

[0031] Please see Figures 1-8 The diagram illustrates a processing device for automated lysis of peripheral blood erythrocytes, comprising: a closed flexible circuit 1 made of medical-grade flexible material, configured with at least one ∞-shaped flow path to guide liquid in a bidirectional circulation, significantly increasing the contact and shear efficiency between the fluid and the inner wall of the flexible circuit 1; an integrated interface 2 on the flexible circuit 1 serving both injection and drainage functions, simplifying the operation; and a pre-packaged solid lysis solution within the flow path ensuring accurate reagent dosage and ease of use, avoiding the risk of contamination from external additions. A rocking platform 4 and its limiting buckle 5 securely support and fix the flexible circuit 1, maintaining its optimal flow path configuration. The drive mechanism 6, directed by the control unit 7, provides a complex composite motion for the rocking stage 4, thereby dynamically changing the spatial orientation of the flexible circuit 1. This drives the blood sample and lysis fluid within the flexible circuit 1 to generate sufficient and irregular turbulence and circulation, ensuring that the lysis fluid dissolves rapidly and comes into full and uniform contact with the blood sample. Ultimately, this achieves efficient and uniform lysis of red blood cells. At the end of the process, the control unit 7 controls the rocking stage 4 to enter the inverted position, preparing it for subsequent sample collection.

[0032] The drive mechanism 6 includes a stepper motor 8, a crank 9 driven by the stepper motor 8, and a connecting rod 10 connected to the crank 9. The rocking shelf 4 is provided with a housing 11 below it. The rocking shelf 4 is connected to the support plate 12 inside the housing 11 through a ball joint 13. The ball head of the ball joint 13 is fixed to the top of the support plate 12, and its ball cup is fixed to the bottom center of the rocking shelf 4 through a connecting column. This design gives the rocking shelf 4 a multi-degree-of-freedom swinging ability. A column 14 is hinged at the bottom of the rocking shelf 4 off-center. The end of the connecting rod 10 away from the crank 9 is rotatably connected to the column 14. When the motor drives the crank 9 and connecting rod 10 mechanism to move, the power is transmitted to the rocking platform 4 through the column 14. Since the fulcrum (ball hinge 13) and the force point (column 14) do not coincide, the rocking platform 4 is forced to perform a composite motion similar to rocking and torturing around the center of the ball hinge 13 in space. This motion is eventually transmitted to the flexible circuit 1 and its internal liquid.

[0033] It is worth noting that the flexible circuit 1 is a one-piece molded annular soft bag with a flat elliptical cross-section, ensuring the airtightness of the flexible circuit 1. When the flexible circuit 1 deforms during shaking, the flat elliptical cross-section can produce a greater rate of change in internal cavity volume and a change in flow channel shape compared to a circular cross-section, thereby generating a stronger squeezing and relaxing effect on the internal liquid, promoting radial mixing of the fluid, breaking the laminar flow state, and making the precipitated blood cells and lysate mix more thoroughly.

[0034] In this technical solution, the column 14 and the connecting rod 10 form an L-shaped structure, which can convert the planar swing motion of the connecting rod 10 into a more complex torque on the rocking platform 4, and intensify the twisting amplitude of the rocking platform 4 around the ball hinge 13, thereby enhancing the strength and complexity of the compound motion, and ultimately improving the mixing effect of the liquid in the flexible circuit 1.

[0035] Furthermore, in the horizontal plane, the length of crank 9 is equal to the distance from column 14 to the center of the rocking platform 4; the length of connecting rod 10 is equal to the distance from the center of the rocking platform 4 to the drive shaft of stepper motor 8; and the length of connecting rod 10 is greater than the length of crank 9. This specific dimensional ratio ensures that when crank 9 makes a circular motion, it can drive the rocking platform 4 through connecting rod 10 to produce a stable amplitude, smooth trajectory, and no dead spots in the compound rocking motion. This design optimizes the power transmission efficiency, avoids motion jamming or impact, and ensures the smoothness and efficiency of the mixing process.

[0036] Because the column 14 swings with multiple degrees of freedom along with the rocking platform 4 during movement, its movement trajectory is a spatial curve. The opening 15 on the support plate 12 provides the necessary movement space for the column 14, preventing it from interfering with or colliding with the fixed support plate 12 during movement. This ensures that the drive mechanism 6 can realize its complete designed movement trajectory without obstruction, guaranteeing the long-term reliability of the equipment.

[0037] In this technical solution, a wedge block 16 is fixedly provided at the bottom of the housing 11, and a stop block 17 is provided at the bottom of the wedge block 16. The lower side of the wedge block 16 is rotatably connected to the base 18 via a pivot. A linear guide rail 19 is fixedly provided at the bottom inner side of the base 18. The linear guide rail 19 is driven by the control unit 7, and the stop block 17 is fixed to the slider of the linear guide rail 19. After the pyrolysis process is completed, the control unit 7 activates the linear guide rail 19, pushing the stop block 17 forward. During the forward movement, the stop block 17 continuously squeezes the inclined surface of the wedge block 16. Utilizing the mechanical principle of the inclined surface, the horizontal thrust is converted into a lifting force, thereby forcing the wedge block 16, along with the entire housing 11 and the rocking platform 4, to rotate around the pivot. This design can smoothly and accurately drive the originally horizontal rocking platform 4 to a vertical state. In this state, the integrated interface 2 of the flexible circuit 1 is naturally located at the lowest point of the entire flexible circuit 1. Utilizing gravity, the sample liquid after pyrolysis can be completely and smoothly collected and discharged through the integrated interface 2, realizing automated sample collection.

[0038] Meanwhile, the limiting buckle 5 includes a pressure block 20. A screw 21 is provided on the outer sides of both ends of the flexible circuit 1. The screw 21 is fixed to the top of the rocking platform 4. Each screw 21 has two nuts 22 threaded onto it. One end of the pressure block 20 is sleeved with the screw 21, and the other end presses against the outer side of the flexible circuit 1. The pressure block 20 is positioned between the two nuts 22. By rotating the two nuts 22, the height position of the pressure block 20 on the screw 21 can be precisely adjusted to accommodate flexible circuits 1 of different thicknesses or with slight manufacturing tolerances. The pressure block 20 ultimately applies pressure to the outer side of the flexible circuit 1, stably pressing it against the surface of the rocking platform 4, preventing it from sliding, shifting, or tilting during the compound motion, ensuring that the ∞-shaped flow path remains constant, thus guaranteeing the consistency of the mixing effect.

[0039] Furthermore, symmetrical protrusions 23 are fixed on the outer sides of both ends of the flexible circuit 1, and the bottom of the pressure block 20 is provided with a recess 24 that mates with the protrusions 23. When the pressure block 20 is pressed down, the protrusions 23 are embedded in the recess 24. This not only more effectively prevents the horizontal movement of the flexible circuit 1, but also resists its torsional deformation during movement, achieving multi-dimensional positioning and fixation. This design greatly enhances the stability of the connection, ensures the efficiency of power transmission from the rocking platform 4 to the flexible circuit 1, and avoids wear or tear that may be caused by local stress concentration in the flexible circuit 1.

[0040] In this technical solution, the pre-packaged solid lysis buffer 3 is an ammonium salt, fixed in tablet form on the inner wall of the flexible circuit 1. Ammonium salt is a classic reagent for lysing red blood cells, and its mechanism of action is mature and reliable. Pre-preparing it into tablets and fixing it on the inner wall of the flexible circuit 1 has multiple functional advantages: First, the dosage is precise, ensuring the stability of the lysis efficiency; second, the solid form facilitates long-term storage and transportation, avoiding the leakage, evaporation, or freezing problems that may exist with liquid lysis buffers; third, when the equipment is started and shaken, the tablets can quickly dissolve and diffuse with the flowing blood, immediately initiating the reaction.

[0041] In this technical solution, the inclined surface of the wedge block 16 is provided with a limiting track that cooperates with the side of the abutment block 17. By limiting the movement trajectory of the abutment block 17, the wedge block 16 is prevented from moving excessively.

[0042] The working principle of the device is as follows:

[0043] First, the operator installs the flexible circuit 1, pre-packaged with solid lysis buffer, onto the rocking platform 4. Using the limiting buckles 5 at both ends and the cooperation of the protrusions 23 and the settling groove 24, the flexible circuit 1 is securely fixed and its preset ∞-shaped flow path is maintained. Then, the peripheral blood sample to be processed is injected into the closed flexible circuit 1 through the injection port in the integrated interface 2 integrated on the flexible circuit 1. After completion, the injection port is closed to ensure the system is in a sealed state.

[0044] After preparation, the operator initiates the lysis procedure via control unit 7. Control unit 7 then commands drive mechanism 6 to start working, stepper motor 8 starts, driving crank 9 to rotate. Crank 9 transmits the rotational motion to column 14, which is hinged to the bottom of rocking platform 4, via connecting rod 10. Since rocking platform 4 is connected to support plate 12 inside housing 11 via ball joint 13, which acts as a universal fulcrum, the driving force from connecting rod 10 and column 14 is transformed into multi-degree-of-freedom composite rocking motion of rocking platform 4 in space. This motion is not a simple reciprocating oscillation, but a complex motion mode that includes rocking and twisting. It drives the blood sample in flexible circuit 1 to generate sufficient, repeated bidirectional circulation and turbulence in the ∞-shaped flow path. This increases the contact area and frequency between blood and solid lysis fluid fixed on the inner wall, promoting rapid dissolution and uniform distribution of the lysis fluid, thereby achieving efficient and uniform lysis of red blood cells. At the same time, the gentle fluid dynamics design avoids excessive shear force, effectively protecting the target mononuclear cells.

[0045] When the preset lysis time ends, the control unit 7 first stops the rocking motion of the drive mechanism 6. Then, the control unit 7 commands the linear guide rail 19 inside the base 18 to move, pushing the abutment block 17 forward. The abutment block 17 contacts the inclined surface of the wedge block 16 at the bottom of the housing 11 and applies a thrust. Since one side of the wedge block 16 is connected to the base 18 via a pivot, this horizontal thrust is converted into a rotational torque, forcing the entire housing 11, along with its internal rocking platform 4 and flexible circuit 1, to rotate around the pivot until the rocking platform 4 becomes vertical (i.e., inverted). In this position, the integrated interface 2 of the flexible circuit 1 is located at the lowest point of the entire flexible circuit 1. At this time, the operator can open the outlet valve on the integrated interface 2, and under the action of gravity, the sample containing the target mononuclear cells after lysis can be smoothly collected into the external container, thus completing the entire automated processing flow.

[0046] As an alternative implementation, the ∞-shaped flow path can also be achieved by connecting an ∞-shaped loop tube to the outside of a main blood bag (not shown in the attached figure), but the one-piece molding solution is superior in terms of mixing efficiency and safety.

[0047] In this design, the movement of the rocking platform 4 is a spatial multi-degree-of-freedom composite motion generated by the drive mechanism 6 below it. This motion can be specifically described as a composite motion combining alternating forward and reverse rotation around an axis with gentle radial swaying.

[0048] Its motion mechanism is as follows: Stepper motor 8 drives crank 9 to perform uniform circular motion, and transmits the power to column 14, which is hinged to the bottom of the rocking platform 4, through connecting rod 10. Since the rocking platform 4 is connected to the support plate 12 through ball joint 13 at its bottom, the ball joint 13 acts as a spatial universal fulcrum, so that the planar driving force from the connecting rod 10 is decomposed and transformed. The following two motions are generated: First, alternating forward and reverse rotation around the axis. The drive mechanism 6 forces the rocking platform 4 to periodically rotate forward and reverse around a virtual axis that passes through the center of the ball joint 13. This action is the main driving force for the liquid to generate bidirectional circulation in the ∞-shaped flexible circuit 1, effectively simulating and replacing the manual inverting and mixing operation. Second, radial gentle shaking. While rotating around the axis, the rocking platform 4 also produces a small-amplitude, irregular radial oscillation and slight bumping. This swaying motion originates from the multiple degrees of freedom provided by the ball hinge 13, which generates gentle turbulence and shear forces in the liquid, equivalent to gentle manual manipulation. This helps to break up aggregated red blood cell clumps and promotes uniform mixing of the lysis buffer and cells, while avoiding severe mechanical impact, thus effectively protecting the target mononuclear cells. This combined motion ensures that the blood sample within the flexible circuit 1 and the pre-packaged solid lysis buffer 3 can achieve rapid, sufficient, and gentle contact and reaction, ultimately achieving a highly efficient and uniform red blood cell lysis effect.

[0049] In addition, such as Figure 3 and Figure 4 As shown, spring members 25 are distributed circumferentially on the outer side of the connecting post between the ball cup of the ball hinge 13 and the rocking shelf 4. One end of the spring member 25 is fixed to the bracket at the top of the support plate 12, and the other end abuts against the connecting post between the ball cup of the ball hinge 13 and the rocking shelf 4. This design can help the rocking shelf 4 return to its original position after radial swinging and slight bumps.

[0050] Meanwhile, the control unit 7 in this utility model is based on conventional programmable controllers, microcontroller systems, or similar industry standard control modules known in the art. The control unit 7 is electrically connected to the stepper motor 8, linear guide rail 19, and other actuators in the drive mechanism 6, and its core function is to execute preset, conventional logic control commands.

[0051] Specifically, the control unit 7 is configured to: during the pyrolysis stage, send a command to the stepper motor 8 to control it to operate at a preset speed and running time, thereby driving the rocking platform 4 to perform the aforementioned compound motion. After pyrolysis is completed, the control unit 7 stops the stepper motor 8 and immediately sends a signal to the driver of the linear guide rail 19 to control it to push the stop block 17, completing the automatic inversion action of the rocking platform 4.

[0052] The control unit 7 itself and the program logic it executes are both mature automation control solutions in the prior art. For example, the control of motor start / stop, forward / reverse rotation and running time, as well as the precise control of the stroke of the linear guide 19, are all conventional programming techniques that can be implemented by those skilled in the art without creative effort. Therefore, in the present invention, the control unit 7 is considered a fully functional prior art component, and its specific internal circuit structure, component models and software code are not the innovation of this invention, and therefore will not be described in detail in the specification. The inventiveness of this invention lies in the aforementioned mechanical structure combination and the synergistic effect it produces.

[0053] Furthermore, the integrated interface 2 used in this invention, which combines the infusion port and outlet valve into a single interface, is a conventional design in existing medical technology. Such integrated interfaces are widely used in disposable medical infusion bags, blood bags, and various closed sample processing containers, aiming to reduce the risk of contamination, simplify operation, and maintain system tightness by reducing the number of interfaces. Therefore, the specific structure, material, and sealing method of this interface can all adopt mature solutions known in the art and are not the innovation of this invention.

[0054] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.

[0055] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A processing device for the automatic lysis of peripheral blood erythrocytes, characterized in that, include: A closed flexible circuit (1), made of medical flexible material, is configured to include at least one ∞-shaped flow path and is provided with an integrated interface (2) that integrates an injection port for injecting blood samples and an outlet valve for discharging lysed samples. Pre-packaged solid pyrolysis fluid (3) is fixed to the inner wall of the ∞-shaped flow path of the flexible circuit (1); A rocking platform (4) is provided with a limiting buckle (5) connected to the flexible circuit (1) to fix and support the flexible circuit (1) so that it maintains the ∞-shaped flow path; The driving mechanism (6) is used to drive the rocking platform (4) to perform compound motion so as to cause the liquid in the flexible circuit (1) to circulate in the ∞-shaped flow path; The control unit (7) is electrically connected to the drive mechanism (6) to control the parameters of the compound motion and to control the rocking platform (4) to rotate to the inverted position after the pyrolysis is completed.

2. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 1, characterized in that: The drive mechanism (6) includes a stepper motor (8), a crank (9) driven by the stepper motor (8), and a connecting rod (10) connected to the crank (9); a housing (11) is provided below the rocking platform (4), and the rocking platform (4) is connected to the support plate (12) inside the housing (11) through a ball hinge (13). The ball head of the ball hinge (13) is fixed to the top of the support plate (12), and its ball cup is fixed to the bottom center of the rocking platform (4) through a connecting column; a column (14) is hinged at the bottom of the rocking platform (4) at a position off-center, and the end of the connecting rod (10) away from the crank (9) is rotatably connected to the column (14).

3. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 1, characterized in that: The flexible circuit (1) is an integrally formed annular soft bag with a flat elliptical cross-section inside.

4. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 2, characterized in that: The column (14) and the connecting rod (10) form an L-shaped structure.

5. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 2, characterized in that: In the horizontal plane, the length of the crank (9) is equal to the distance from the column (14) to the center of the rocking platform (4); the length of the connecting rod (10) is equal to the distance from the center of the rocking platform (4) to the drive shaft of the stepper motor (8); the length of the connecting rod (10) is greater than the length of the crank (9).

6. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 2, characterized in that: The support plate (12) has an opening (15) to accommodate the range of motion of the column (14).

7. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 2, characterized in that: The bottom of the housing (11) is fixedly provided with a wedge block (16), and the bottom of the wedge block (16) is provided with a stop block (17); the lower side of the wedge block (16) is rotatably connected to the base (18) through a rotating shaft; the bottom of the inner side of the base (18) is fixedly provided with a linear guide rail (19), the linear guide rail (19) is driven by the control unit (7), and the stop block (17) is fixed to the slider of the linear guide rail (19); The linear guide rail (19) drives the abutment (17) to move toward the wedge block (16), squeezing the inclined surface at the bottom of the wedge block (16), thereby causing the wedge block (16) and the entire housing (11) to rotate together until the rocking platform (4) is rotated to a vertical state, so that the integrated interface (2) is located below the flexible circuit (1).

8. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 1, characterized in that: The pre-packaged solid pyrolysis solution (3) is an ammonium salt, which is fixed in tablet form on the inner wall of the flexible circuit (1).

9. The processing device for automatic lysis of peripheral blood erythrocytes according to claim 1, characterized in that: The limiting buckle (5) includes a pressure block (20). A screw (21) is provided on the outer side of both ends of the flexible circuit (1). The screw (21) is fixed to the top of the rocking platform (4). Two nuts (22) are threaded on each screw (21). One end of the pressure block (20) is sleeved with the screw (21), and the other end is pressed on the outer side of the flexible circuit (1). The pressure block (20) is located between the two nuts (22). By adjusting the height of the two nuts (22), the two pressure blocks (20) are used to limit and fix the flexible circuit (1).

10. A processing device for automatic lysis of peripheral blood erythrocytes according to claim 9, characterized in that: The flexible circuit (1) has symmetrical protrusions (23) fixed on the outer sides at both ends, and the bottom of the pressure block (20) has a groove (24) that cooperates with the protrusions (23).

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