A reagent strip for liquid transfer function by cavity pressure

Abstract

The utility model discloses a reagent strip that realizes liquid transfer function through cavity pressure, including substrate, provide support for integral structure, substrate can be independently formed single -channel reagent strip substrate, or integrated multichannel structure forms reagent plate substrate, adapts multihole plate detection equipment, and cavity group is integrated on the substrate surface, is used for storing reagent, and pressure liquid transfer device is the upper and lower split cavity structure, and the lower cavity is used for storing the reagent to be transferred, and the upper cavity can be used for depositing magnetic separation magnetic beads, and movable rubber stopper is equipped between the upper and lower cavities. The pressure change of rubber stopper control drives liquid to flow between different cavities, has the advantages that compact structure, easy operation, strong adaptability, high transfer efficiency, is suitable for nucleic acid detection, immunological analysis and other instant detection scene.

Description

Technical Field

[0001] This utility model belongs to the technical field of in vitro diagnostic reagent strips, specifically relating to a reagent strip that achieves liquid transfer through cavity pressure. Background Technology

[0002] Currently, the two mainstream technologies in the automated nucleic acid extraction market are fully automated nucleic acid extraction based on the magnetic rod method and fully automated nucleic acid extraction based on the aspiration method (pipette method). The magnetic rod method, by using movable and washable magnetic rod sleeves to transfer magnetic beads, achieves high-throughput, low-cost, and low-risk cross-contamination nucleic acid extraction, making it one of the preferred technologies for large-scale, industrialized nucleic acid extraction projects. The magnetic rod method typically employs batch simultaneous detection, where multiple test reagents are loaded into different detection slots on a deep-well plate, and then the deep-well plate is placed in the detection device for testing. However, because the corresponding device lacks a pipetting function, it cannot achieve mixing of different reagents, which greatly limits the instrument's application range. Existing technologies, particularly the reagent strips used with this method, suffer from the following key drawbacks: first, the liquid transfer device is designed separately from the reagent strip, requiring additional operations to add reagents that need immediate mixing, a cumbersome process prone to contamination, contradicting automation requirements; second, the transfer device has a fixed structure, making it impossible to choose between integrated or separate assembly based on detection needs, resulting in poor flexibility.

[0003] In view of the above factors, developing a pressure-controlled reagent strip that is compatible with independent / reagent plate forms and integrated / split pressure transfer devices has important practical value. Utility Model Content

[0004] The purpose of this invention is to provide a reagent strip that achieves liquid transfer through cavity pressure, in order to solve the problems mentioned in the background art.

[0005] The purpose of this utility model is achieved through the following technical solution: a reagent strip that achieves liquid transfer function through cavity pressure, including a substrate, the substrate providing support for the overall structure, the substrate can be independently formed as a single-channel reagent strip substrate, or integrated into a multi-channel structure to form a reagent plate substrate including at least 2 channels.

[0006] A cavity assembly is provided along the transverse direction of the substrate, and the cavity assembly is integrated on the surface of the substrate for storing reagents;

[0007] It also includes a pressure liquid transfer device, which has an upper and lower chamber structure. The lower chamber of the pressure liquid transfer device is used to store the reagent to be transferred, and the upper chamber of the pressure liquid transfer device is used to store magnetic separation beads. A movable rubber stopper is provided between the upper and lower chambers.

[0008] Furthermore, the pressure liquid transfer device is integrally injection molded with the substrate, and the substrate is independently molded as a single-channel reagent strip substrate.

[0009] Furthermore, the pressure liquid transfer device is injection molded separately, and the pressure liquid transfer device is detachably connected to the substrate.

[0010] Furthermore, the top of the movable rubber stopper has a U-shaped structure, and the movable rubber stopper can move downward along the pressure liquid transfer device.

[0011] Furthermore, a magnetic bead is stored in the U-shaped structure at the top of the movable rubber stopper. The magnetic bead is attracted to the surface of the external instrument magnetic rod. As the magnetic rod continues to descend, it presses down on the movable rubber stopper, causing the pressure at the bottom of the movable rubber stopper to increase and the pressure sensing membrane to rupture.

[0012] Furthermore, the detachable connection between the pressure liquid transfer device and the substrate is a plug-in connection.

[0013] Furthermore, symmetrical positioning protrusions are fixedly provided on the substrate, and the pressure liquid transfer device is installed in the reserved hole of the reagent plate substrate through the positioning protrusions.

[0014] Furthermore, the inner wall of the upper cavity of the pressure liquid transfer device is coated with an anti-adsorption coating, which is a polyethylene glycol coating.

[0015] Furthermore, a liquid outlet is fixedly provided at the bottom of the lower cavity of the pressure liquid transfer device, and a pressure sensing membrane is attached to the outer wall of the liquid outlet.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] This invention is highly adaptable: it can be implemented in two forms, namely, independent reagent strips and multi-channel reagent plates, to suit both single-sample rapid detection and high-throughput batch detection scenarios; the split-type transfer device can be flexibly replaced to meet the needs of multiple tests.

[0018] This invention offers precise and efficient transfer: the compartmentalized design prevents magnetic beads from agglomerating with reagents, thus improving the utilization rate of magnetic beads; the flow-guiding groove reduces liquid residue; and multi-channel synchronous transfer enhances detection efficiency.

[0019] This utility model is flexible and convenient to assemble: the one-piece molding is suitable for standardized production, and the split design is suitable for multiple projects and easy to replace; no professional tools are required, and the installation of the split device can be completed manually, making the operation threshold low.

[0020] This invention offers compatibility and safety features: it is highly compatible with magnetic rod method instruments, and the anti-adsorption coating on the magnetic beads ensures detection accuracy, meeting in vitro diagnostic safety standards.

[0021] This invention drives the flow of liquid between different cavities by controlling pressure changes with a rubber stopper. It has the advantages of compact structure, simple operation, strong adaptability and high transfer efficiency, and is suitable for point-of-care testing scenarios such as nucleic acid detection and immunoassay. Attached Figure Description

[0022] Figure 1 This is a three-dimensional schematic diagram of the present invention;

[0023] Figure 2 This is a utility model Figure 1 Plan view;

[0024] Figure 3 This is a schematic diagram of the pressure liquid transfer device of this utility model and its mating with the base plate;

[0025] Figure 4 This is a schematic diagram of the single-channel reagent strip of this utility model;

[0026] Figure 5 This is a schematic diagram of the pressure-sensing membrane in the pressure liquid transfer device of this utility model;

[0027] Figure 6 This is a schematic diagram of the pressure liquid transfer device of this utility model with a liquid outlet;

[0028] Figure 7 This is a utility model Figure 5 A cross-sectional schematic diagram. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0030] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0031] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0032] like Figure 1-7 As shown, a reagent strip that achieves liquid transfer function through cavity pressure includes a substrate 1, which provides support for the overall structure. The substrate 1 can be independently formed as a single-channel reagent strip substrate, or integrated into a multi-channel structure to form a reagent plate substrate with at least two channels.

[0033] A cavity assembly 2 is provided along the transverse direction of the substrate 1, and the cavity assembly 2 is integrated on the surface of the substrate 1 for storing reagents;

[0034] It also includes a pressure liquid transfer device 3, which has an upper and lower chamber structure. The lower chamber 4 of the pressure liquid transfer device 3 is used to store the reagent to be transferred, and the upper chamber 5 of the pressure liquid transfer device 3 is used to store magnetic separation beads. A movable rubber stopper 6 is provided between the upper chamber 5 and the lower chamber 4.

[0035] To facilitate the use of both independent reagent strips and multi-channel reagent plates, adapting to scenarios of rapid single-sample detection and high-throughput batch detection, the pressure liquid transfer device 3 and the substrate 1 are integrally injection molded, with the substrate 1 being an independently formed single-channel reagent strip substrate.

[0036] Alternatively, the pressure liquid transfer device 3 may be injection molded separately, and the pressure liquid transfer device 3 may be detachably connected to the substrate 1.

[0037] To facilitate the collection of magnetic beads during use, the top of the movable rubber stopper 6 has a U-shaped structure, and the movable rubber stopper 6 can move downward along the pressure liquid transfer device 3.

[0038] The movable rubber stopper 6 has a U-shaped structure at the top that houses a magnetic bead. The magnetic bead is attracted to the surface of the external instrument magnetic rod. As the magnetic rod continues to descend, it presses down on the movable rubber stopper 6, causing the pressure at the bottom of the movable rubber stopper 6 to increase and the pressure sensing membrane to rupture.

[0039] To facilitate flexible replacement of the split-type transfer device during use and meet the needs of multiple testing items, the detachable connection between the pressure liquid transfer device 3 and the base plate 1 is a plug-in connection.

[0040] To facilitate the fixing of the pressure liquid transfer device 3 during use, symmetrical positioning protrusions 7 are fixedly provided on the substrate 1. The pressure liquid transfer device 3 is installed in the reserved hole of the reagent plate substrate through the positioning protrusions 7.

[0041] To prevent magnetic beads from adhering during use, the inner wall of the upper cavity 5 of the pressure liquid transfer device 3 is coated with an anti-adsorption coating, which is a polyethylene glycol coating.

[0042] The lower chamber 4 of the pressure liquid transfer device 3 is fixedly provided with a liquid outlet 8. A pressure sensing membrane 9 is attached to the outer wall of the liquid outlet 8, and a flow guiding groove is provided at the bottom of the lower chamber 4. The flow guiding groove is inclined to ensure that the liquid is completely discharged from the lower chamber 4.

[0043] Taking a single-channel reagent strip combined with a magnetic rod method instrument as an example, the initialization stage is as follows: place one single-channel reagent strip in the instrument slot (not shown in the instrument slot diagram, which is existing technology), and install the split pressure transfer device in the reserved hole of the reagent plate through the positioning protrusion.

[0044] Magnetic bead separation and reagent transfer: The instrument's magnetic rod descends to the position of the magnetic bead above the rubber stopper. The magnetic bead is attracted to the surface of the magnetic rod. The magnetic rod continues to descend, pressing the piston. The increased pressure at the bottom of the piston causes the pressure sensing membrane to rupture, and the reagent at the bottom of the piston flows into the next chamber through the liquid outlet.

[0045] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0046] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A reagent strip that achieves liquid transfer through cavity pressure, characterized in that: Includes a substrate (1), which provides support for the overall structure. The substrate (1) can be independently formed as a single-channel reagent strip substrate, or integrated into a multi-channel structure to form a reagent plate substrate with at least two channels. A cavity assembly (2) is provided along the transverse direction of the substrate (1), the cavity assembly (2) is integrated on the surface of the substrate (1), and is used to store reagents; It also includes a pressure liquid transfer device (3), which has an upper and lower chamber structure. The lower chamber (4) of the pressure liquid transfer device (3) is used to store the reagent to be transferred, and the upper chamber (5) of the pressure liquid transfer device (3) is used to store magnetic separation beads. A movable rubber stopper (6) is provided between the upper chamber (5) and the lower chamber (4).

2. The reagent strip according to claim 1, which achieves liquid transfer through cavity pressure, is characterized in that: The pressure liquid transfer device (3) and the substrate (1) are integrally injection molded, and the substrate (1) is independently molded as a single-channel reagent strip substrate.

3. The reagent strip according to claim 1, which achieves liquid transfer through cavity pressure, is characterized in that: The pressure liquid transfer device (3) is injection molded separately, and the pressure liquid transfer device (3) is detachably connected to the substrate (1).

4. The reagent strip that achieves liquid transfer through cavity pressure according to claim 2 or 3, characterized in that: The top of the movable rubber stopper (6) has a U-shaped structure, and the movable rubber stopper (6) can move downward along the pressure liquid transfer device (3).

5. The reagent strip according to claim 4, which achieves liquid transfer through cavity pressure, is characterized in that: The movable rubber stopper (6) has a U-shaped structure at the top containing a magnetic bead. The magnetic bead is attracted to the surface of the external instrument magnetic rod. The magnetic rod continues to descend and press the movable rubber stopper (6). The pressure at the bottom of the movable rubber stopper (6) increases, causing the pressure sensing membrane to rupture.

6. The reagent strip that achieves liquid transfer through cavity pressure according to claim 3, characterized in that: The detachable connection between the pressure liquid transfer device (3) and the substrate (1) is a plug-in connection.

7. The reagent strip according to claim 5, which achieves liquid transfer through cavity pressure, is characterized in that: Symmetrical positioning protrusions (7) are fixedly provided on the substrate (1). The pressure liquid transfer device (3) is installed in the reserved hole of the reagent plate substrate through the positioning protrusions (7).

8. The reagent strip according to claim 2, which achieves liquid transfer through cavity pressure, is characterized in that: The inner wall of the upper cavity (5) of the pressure liquid transfer device (3) is coated with an anti-adsorption coating, which is a polyethylene glycol coating.

9. The reagent strip according to claim 7, which achieves liquid transfer through cavity pressure, is characterized in that: The lower chamber (4) of the pressure liquid transfer device (3) is fixedly provided with a liquid outlet (8), and a pressure sensing membrane (9) is attached to the outer wall of the liquid outlet (8).

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