A fermentation apparatus for sodium hyaluronate

Abstract

The utility model belongs to sodium hyaluronate fermentation technical field, concretely relates to a kind of sodium hyaluronate fermentation equipment, including fermentation tank mechanism, the fermentation tank mechanism includes support frame, the inside fixed connection of support frame has the fermentation tank of built-in temperature sensor, the bottom of the inner chamber of fermentation tank is provided with discharge pipe, the inner wall of fermentation tank is fixedly connected with aeration ring, the inner chamber of aeration ring is provided with gas supply pipe on one side;Stirring mechanism, the stirring mechanism includes tank cover, the top of tank cover is fixedly connected with motor, the water in the water storage cavity is directly heated to boiling by semiconductor heat exchanger, independently generates sterilization steam, without additional equipment, not only simplify sterilization operation process, also reduce equipment procurement and maintenance cost;For the problem that sterilization and temperature control system have no linkage, complex structure, can give consideration to the double functions of "steam production when sterilizing" and "temperature control when fermenting".

Description

Technical Field

[0001] This utility model relates to the field of sodium hyaluronate fermentation technology, specifically to a sodium hyaluronate fermentation device. Background Technology

[0002] Traditional sodium hyaluronate fermentation equipment is mainly used in the field of bio-fermentation. It is the core equipment for the metabolic synthesis of sodium hyaluronate by microorganisms such as streptococci. It can meet the basic fermentation requirements of pharmaceutical-grade and cosmetic-grade sodium hyaluronate. By providing aeration, stirring and basic temperature control environment, it ensures the normal metabolism of microorganisms and provides qualified fermentation broth for subsequent product extraction and purification.

[0003] In the large-scale fermentation of high-purity sodium hyaluronate, traditional equipment requires additional steam generators for sterilization before fermentation, which is cumbersome and costly. During fermentation, temperature control relies on independent components without linkage with the sterilization system. This not only results in a complex structure and large space occupation, but also easily leads to large temperature differences inside the tank due to insufficient temperature control accuracy, affecting the stability of cell metabolism. It is difficult to achieve the multi-functional requirement of "sterilization-temperature control" integration, thus restricting the improvement of production efficiency. Utility Model Content

[0004] This invention provides a fermentation device for sodium hyaluronate to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows: A fermentation device for sodium hyaluronate includes a fermentation tank mechanism, which includes a support frame. A fermentation tank with a built-in temperature sensor is fixedly connected inside the support frame. A discharge pipe is provided at the bottom of the fermentation tank's inner cavity. An aeration ring is fixedly connected to the inner wall of the fermentation tank, and an air supply pipe is provided on one side of the aeration ring's inner cavity. A stirring mechanism includes a tank lid, with a motor fixedly connected to the top of the lid. A rotating rod is fixedly connected to the lower end of the motor shaft, and stirring blades are fixedly connected to the surface of the rotating rod. A multi-functional temperature control mechanism includes a water storage chamber, one side of which is fixedly connected to the surface of the fermentation tank. A semiconductor heat exchanger is provided on one side of the water storage chamber's inner cavity.

[0006] A further improvement of this utility model is that the stirring mechanism further includes a feed pipe, which is disposed on one side of the top of the tank lid.

[0007] A further improvement of this utility model is that: a defoamer addition tube is provided on the other side of the top of the can lid, and a pressure relief valve is also provided inside the can lid.

[0008] A further improvement of this utility model is that a steam conveying pipe is provided on one side of the top of the water storage cavity, and one end of the steam conveying pipe is connected to the inside of the tank cover.

[0009] A further improvement of this utility model is that the multifunctional temperature control mechanism also includes a water inlet, which is located at the top of the water storage chamber.

[0010] A further improvement of this utility model is that a first connecting pipe is provided at the bottom of the inner cavity of the water storage chamber, and a circulation pump is provided inside the first connecting pipe.

[0011] A further improvement of this utility model is that: a circulating temperature control pipe is connected to the end of the first connecting pipe, and a second connecting pipe is connected to one end of the circulating temperature control pipe, and the circulating temperature control pipe is installed in the interlayer of the fermenter body.

[0012] A further improvement of this utility model is that one end of the second connecting pipe is connected to the top of the inner cavity of the water storage chamber, and an electromagnetic valve is installed inside the steam conveying pipe.

[0013] Due to the adoption of the above technical solution, the technological progress achieved by this utility model compared to the prior art is as follows: This invention provides a fermentation device for sodium hyaluronate. It directly heats the water in the storage chamber to boiling point via a semiconductor heat exchanger, autonomously generating sterilizing steam without the need for additional equipment. This simplifies the sterilization process and reduces equipment procurement and maintenance costs. Addressing the issues of incoordination and complex structures in sterilization and temperature control systems, this device integrates the dual functions of "steam generation during sterilization" and "temperature control during fermentation." Combined with the storage chamber and circulating temperature control pipe, it forms an integrated system, eliminating the need for a separate temperature control component and significantly simplifying the equipment structure and reducing space requirements. Simultaneously, the circulating temperature control pipe circulates heat through the constant-temperature medium in the storage chamber, significantly reducing the temperature difference within the tank and ensuring stable microbial metabolism. Ultimately, this achieves the multi-functional requirement of integrated "sterilization-temperature control," reducing operational steps and improving temperature control accuracy. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the main structure of this utility model; Figure 2 This is a side view of the present invention. Figure 3 This is a schematic diagram of the stirring mechanism and the multifunctional temperature control mechanism of this utility model; Figure 4 This is a schematic diagram of the stirring mechanism of this utility model; Figure 5 This is an exploded view of the multifunctional temperature control mechanism of this utility model; Figure 6This is a schematic diagram of the structure of the aeration ring and aeration pipe of this utility model.

[0015] In the diagram: 11. Support frame; 12. Fermentation tank; 13. Discharge pipe; 14. Aeration ring; 15. Air supply pipe; 21. Tank lid; 22. Motor; 23. Rotating rod; 24. Stirring blade; 25. Feed pipe; 26. Defoamer addition pipe; 27. Pressure relief valve; 31. Water storage chamber; 32. Semiconductor heat exchanger; 33. Steam delivery pipe; 34. Water inlet; 35. First connecting pipe; 36. Circulation pump; 37. Circulation temperature control pipe; 38. Second connecting pipe. Detailed Implementation

[0016] The present invention will be further described in detail below with reference to embodiments: Example 1, as Figures 1-6 As shown, this utility model provides a fermentation device for sodium hyaluronate, including a fermentation tank mechanism, which includes a support frame 11. A fermentation tank 12 with a built-in temperature sensor is fixedly connected inside the support frame 11. A discharge pipe 13 is provided at the bottom of the inner cavity of the fermentation tank 12. An aeration ring 14 is fixedly connected to the inner wall of the fermentation tank 12. An air supply pipe 15 is provided on one side of the inner cavity of the aeration ring 14. A stirring mechanism includes a tank cover 21. A motor 22 is fixedly connected to the top of the tank cover 21. A rotating rod 23 is fixedly connected to the lower end of the rotating shaft of the motor 22. A stirring blade 24 is fixedly connected to the surface of the rotating rod 23. A multi-functional temperature control mechanism includes a water storage chamber 31. One side of the water storage chamber 31 is fixedly connected to the surface of the fermentation tank 12. A semiconductor heat exchanger 32 is provided on one side of the inner cavity of the water storage chamber 31.

[0017] In this embodiment, an appropriate amount of water is injected into the water storage chamber 31 through the water inlet 34, and the semiconductor heat exchanger 32 of the multi-functional temperature control mechanism is activated. Its heating end heats the water in the water storage chamber 31 to boiling, and the generated high-temperature steam enters the fermenter 12 through the steam delivery pipe 33. The on-off state is controlled by the solenoid valve to sterilize the inside of the tank and components such as the stirring blade 24 and the aeration ring 14 at high temperature. During the sterilization process, the pressure inside the tank is kept stable through the pressure relief valve 27 to avoid overpressure and ensure thorough sterilization.

[0018] Example 2, as Figures 1-6 As shown, based on Embodiment 1, this utility model provides a technical solution: Preferably, the stirring mechanism further includes a feed pipe 25, which is disposed on one side of the top of the tank cover 21. A defoamer adding pipe 26 is disposed on the other side of the top of the tank cover 21. A pressure relief valve 27 is also disposed inside the tank cover 21. A steam conveying pipe 33 is disposed on one side of the top of the inner cavity of the water storage chamber 31. One end of the steam conveying pipe 33 is connected to the inside of the tank cover 21. The multi-functional temperature control mechanism further includes a water inlet 34, which is disposed on the top of the inner cavity of the water storage chamber 31.

[0019] In this embodiment, after sterilization, the semiconductor heat exchanger 32 is turned off. After the fermentation tank 12 cools down to the initial temperature required for fermentation, such as 30-32°C, the sterilized culture medium containing glucose, yeast extract, and streptococcal strains is added into the fermentation tank 12 through the feed pipe 25. After the raw material addition is completed, the stirring mechanism and aeration system are started. The motor 22 drives the rotating rod 23 to rotate the stirring blades 24, so that the culture medium and the strains are mixed evenly. At the same time, the external air supply device introduces sterile air into the aeration ring 14 through the air supply pipe 15. The aeration ring 14 releases bubbles along the inner wall of the fermentation tank to provide dissolved oxygen for the metabolism of the bacteria. The stirring also improves the uniformity of dissolved oxygen.

[0020] Example 3, as Figures 1-6 As shown, based on Embodiment 1, this utility model provides a technical solution: Preferably, a first connecting pipe 35 is provided at the bottom of the inner cavity of the water storage chamber 31, a circulation pump 36 is provided inside the first connecting pipe 35, a circulation temperature control pipe 37 is connected to the end of the first connecting pipe 35, a second connecting pipe 38 is connected to one end of the circulation temperature control pipe 37, the circulation temperature control pipe 37 is provided in the tank body interlayer of the fermentation tank 12, one end of the second connecting pipe 38 is connected to the top of the inner cavity of the water storage chamber 31, and a solenoid valve is provided inside the steam conveying pipe 33.

[0021] In this embodiment, the semiconductor heat exchanger 32 switches to temperature control mode, and the water temperature in the water storage chamber 31 is adjusted according to the temperature required for cell metabolism. The circulation pump 36 is started, and the water in the water storage chamber 31 enters the circulation temperature control pipe 37 through the first connecting pipe 35. It exchanges heat with the fermenter through the pipe wall in the inner jacket of the fermenter 12. The temperature inside the fermenter is adjusted according to the temperature data measured by the temperature sensor built into the fermenter 12. The heat-exchanged medium flows back to the water storage chamber 31 through the second connecting pipe 38, forming a closed loop circulation and reducing the temperature difference inside the tank. During the fermentation process, if excessive foam is generated inside the tank, defoamer is added through the defoamer addition pipe 26. The pressure relief valve 27 balances the pressure inside the tank in real time to ensure a stable fermentation environment. After the fermentation is completed, all systems are shut down, and the fermentation liquid containing sodium hyaluronate is discharged through the discharge pipe 13, completing the entire fermentation process.

[0022] The working principle of the sodium hyaluronate fermentation equipment will be explained in detail below.

[0023] like Figures 1-6As shown, an appropriate amount of water is injected into the water storage chamber 31 through the water inlet 34. The semiconductor heat exchanger 32 of the multi-functional temperature control mechanism is activated, and its heating end heats the water in the water storage chamber 31 to boiling. The generated high-temperature steam enters the fermenter 12 through the steam delivery pipe 33 and is controlled by the solenoid valve to sterilize the inside of the tank and components such as the stirring blades 24 and the aeration rings 14 at high temperature. During the sterilization process, the pressure inside the tank is maintained stable through the pressure relief valve 27 to avoid overpressure and ensure thorough sterilization. After sterilization, the semiconductor heat exchanger 32 is turned off. After the fermenter 12 cools down to the initial temperature required for fermentation, such as 30-32°C, the sterilized culture medium containing glucose, yeast extract, and streptococcal inoculum is added into the fermenter 12 through the feed pipe 25. After the raw material addition is completed, the stirring mechanism and aeration system are activated. The motor 22 drives the rotating rod 23 to rotate the stirring blades 24, so that the culture medium and inoculum are mixed evenly. At the same time, the external The gas supply equipment introduces sterile air into the aeration ring 14 through the gas supply pipe 15. The aeration ring 14 releases bubbles along the inner wall of the fermenter to provide dissolved oxygen for bacterial metabolism. Stirring enhances the uniformity of dissolved oxygen. The semiconductor heat exchanger 32 switches to temperature control mode and adjusts the water temperature in the water storage chamber 31 according to the temperature required for bacterial metabolism. The circulation pump 36 is started, and the water in the water storage chamber 31 enters the circulation temperature control pipe 37 through the first connecting pipe 35. It exchanges heat with the fermenter 12 through the pipe wall to regulate the temperature inside the tank. The medium after heat exchange flows back to the water storage chamber 31 through the second connecting pipe 38 to form a closed loop circulation and reduce the temperature difference inside the tank. During fermentation, if excessive foam is generated in the tank, defoamer is precisely added through the defoamer addition pipe 26. The pressure relief valve 27 balances the pressure inside the tank in real time to ensure a stable fermentation environment. After fermentation, all systems are shut down, and the fermentation liquid containing sodium hyaluronate is discharged through the discharge pipe 13, completing the entire fermentation process.

[0024] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the protection scope of the present invention.

Claims

1. A fermentation apparatus for sodium hyaluronate, characterized in that: include The fermentation tank mechanism includes a support frame (11), a fermentation tank (12) with a built-in temperature sensor is fixedly connected inside the support frame (11), a discharge pipe (13) is provided at the bottom of the inner cavity of the fermentation tank (12), an aeration ring (14) is fixedly connected to the inner wall of the fermentation tank (12), and an air supply pipe (15) is provided on one side of the inner cavity of the aeration ring (14). The stirring mechanism includes a tank cover (21), a motor (22) is fixedly connected to the top of the tank cover (21), a rotating rod (23) is fixedly connected to the lower end of the rotating shaft of the motor (22), and a stirring blade (24) is fixedly connected to the surface of the rotating rod (23). The multifunctional temperature control mechanism includes a water storage chamber (31), one side of which is fixedly connected to the surface of the fermenter (12), and a semiconductor heat exchanger (32) is provided on one side of the inner cavity of the water storage chamber (31).

2. The fermentation equipment for sodium hyaluronate according to claim 1, characterized in that: The stirring mechanism also includes a feed pipe (25), which is located on one side of the top of the tank cover (21).

3. The fermentation equipment for sodium hyaluronate according to claim 1, characterized in that: A defoamer addition tube (26) is provided on the other side of the top of the can lid (21), and a pressure relief valve (27) is also provided inside the can lid (21).

4. The fermentation equipment for sodium hyaluronate according to claim 1, characterized in that: A steam delivery pipe (33) is provided on one side of the top of the water storage cavity (31), and one end of the steam delivery pipe (33) is connected to the inside of the tank cover (21).

5. The fermentation equipment for sodium hyaluronate according to claim 4, characterized in that: The multi-functional temperature control mechanism also includes a water inlet (34), which is located at the top of the inner cavity of the water storage chamber (31).

6. The fermentation equipment for sodium hyaluronate according to claim 4, characterized in that: The bottom of the water storage chamber (31) is provided with a first connecting pipe (35), and a circulation pump (36) is provided inside the first connecting pipe (35).

7. The fermentation equipment for sodium hyaluronate according to claim 6, characterized in that: The first connecting pipe (35) is connected to a circulating temperature control pipe (37) at one end, and a second connecting pipe (38) is connected to one end of the circulating temperature control pipe (37). The circulating temperature control pipe (37) is installed in the tank body interlayer of the fermenter (12).

8. The fermentation equipment for sodium hyaluronate according to claim 7, characterized in that: One end of the second connecting pipe (38) is connected to the top of the inner cavity of the water storage chamber (31), and an electromagnetic valve is installed inside the steam conveying pipe (33).

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