Closed transfer culture device

Abstract

The utility model relates to the field of biotechnology discloses a kind of closed transfer culture devices, comprising: culture plate, culture cavity is equipped in it, the liquid inlet, cell effluent port and waste liquid effluent port being equipped on the culture plate are communicated with the culture cavity;Culture solution replenishing bottle is used to provide culture solution for the culture cavity, and the culture solution replenishing bottle is connected with the liquid inlet by liquid supplementing pipe;Cell collection bag is connected with the cell effluent port by first collection pipe, and the cell collection bag is connected with power device to be used to collect the cell of culture completion;Waste liquid recovery bag is connected with the waste liquid effluent port by second collection pipe, and the waste liquid recovery bag is connected with power device to be used to collect the waste liquid generated by culture.The utility model realizes that cell culture and recovery whole process is isolated from outside world, whole process is sterile culture and small space is occupied.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology, and in particular to a closed transfer culture device. Background Technology

[0002] Cell culture requires consumables such as culture dishes, culture plates, culture flasks, multilayer culture flasks, and cell factories. The trend towards space cell culture experiments is growing stronger, as the space environment presents significant challenges. Space cell culture experiments rely heavily on cell culture consumables, and the requirements for these consumables far exceed those of ground-based laboratories. In addition to the standard requirements of high cleanliness and functional suitability, they must meet several core standards for the extreme environment of space. For example, space experimental consumables must possess high strength to withstand the vibrations and impacts during launch and the challenges of the space environment, ensuring stable and reliable performance throughout the mission without breakage or deformation, thus avoiding any impact on experimental results. Current consumables such as culture plates, culture flasks, and cell factories are bulky and cannot achieve fully closed, aseptic culture operations. Manual sampling is required to observe cell growth during the culture process, making cell collection inconvenient. Utility Model Content

[0003] The purpose of this invention is to provide a closed transfer culture device that isolates the cell culture and recovery process from the outside world, ensuring sterile culture throughout and occupying little space.

[0004] To achieve the above objectives, this utility model provides a closed transfer culture device, comprising:

[0005] A culture plate having a culture chamber inside, the culture plate having a liquid inlet, a cell outlet and a waste liquid outlet communicating with the culture chamber;

[0006] A culture medium replenishment bottle is used to provide culture medium to the culture chamber, and the culture medium replenishment bottle is connected to the inlet via a replenishment tube;

[0007] A cell collection bag is connected to the cell outlet via a first collection tube, and the cell collection bag is connected to a power device for collecting cells that have completed culture.

[0008] The waste liquid recovery bag is connected to the waste liquid outlet via a second collection pipe, and the waste liquid recovery bag is connected to a power unit for collecting the waste liquid generated during cultivation.

[0009] As a preferred embodiment of this utility model, the culture plate includes an upper plate, a lower plate, a filter, and a main controller. Both the lower plate and the upper plate are provided with culture tanks. The lower plate is connected to the upper plate, and the culture tanks of the lower plate and the upper plate form the culture chamber. The culture plate is provided with a vent communicating with the culture chamber. The vent is connected to the filter through a vent pipe. The lower plate is provided with a mounting groove for mounting the main controller. The main controller is connected to an external cell monitoring instrument through a data transmission line.

[0010] As a preferred embodiment of this utility model, the culture chamber is provided with two sets of buffer strips that divide the culture chamber into two buffer zones and a culture zone. The culture zone is sandwiched between the two buffer zones, and the buffer strips are provided with ports for connecting the culture zone and the buffer zones. The liquid inlet and the waste liquid outlet are respectively connected to the two ends of one of the buffer zones, and the vent and the cell outlet are respectively connected to the two ends of the other buffer zone.

[0011] As a preferred embodiment of this utility model, the replenishment tube, the first collection tube, and the second collection tube are all equipped with flow-stopping clamps.

[0012] As a preferred embodiment of this utility model, the lower plate is provided with positioning protrusions at the four corners, the upper plate is provided with positioning grooves at the four corners that cooperate with the positioning protrusions, the culture tank edge of the upper plate is provided with trapezoidal ultrasonic lines, and the upper plate and the lower plate are ultrasonically connected.

[0013] As a preferred embodiment of this utility model, the culture medium replenishment bottle includes a bottle body and a bottle cap. The bottle cap is fitted onto the bottle mouth of the bottle body. The bottle cap is provided with an air tube that communicates with the inner cavity of the bottle body. The end of the air tube is provided with a needle filter. The needle filter is connected to a syringe. The replenishment tube passes through the bottle cap and extends into the bottom of the bottle body.

[0014] As a preferred embodiment of this utility model, the upper part of the bottle cap is provided with an air tube interface and a liquid tube interface. The air tube is inserted into the air tube interface. The bottom of the bottle cap is provided with an inner interface that communicates with the liquid tube interface. Two arc-shaped convex rings are provided at intervals on the inner wall of the inner interface. The liquid replenishment tube includes an outer tube and an inner tube. The inner tube is inserted into the inner interface. One end of the outer tube is inserted into the liquid tube interface. The other end of the outer tube is connected to the liquid inlet through a Luer connector.

[0015] As a preferred embodiment of this utility model, the inner tube is a Teflon tube.

[0016] As a preferred embodiment of this utility model, the power device is a syringe, the cell collection bag is provided with a first collection air tube, the end of the first collection air tube is provided with a needle filter connected to the syringe, the waste liquid recovery bag is provided with a second collection air tube, the end of the second collection air tube is provided with a needle filter connected to the syringe, and both the first collection air tube and the second collection air tube are provided with flow stop clamps.

[0017] As a preferred embodiment of this utility model, the first collection tube is connected to the cell outlet via a Luer connector, and the second collection tube is connected to the waste liquid outlet via a Luer connector.

[0018] Compared with the prior art, the closed transfer culture device of this utility model has the following advantages:

[0019] In this invention, the culture medium replenishment bottle provides nutrients to the culture chamber within the culture plate via a replenishment tube. After culture is completed, the cells in the culture chamber are transferred to the cell collection bag via a power device on the cell collection bag in conjunction with the first collection tube. Similarly, the waste liquid in the culture chamber is transferred to the waste liquid collection bag via a power device on the waste liquid recovery bag in conjunction with the second collection tube. This ensures that the cell culture and recovery process is completely isolated from the outside environment, meeting the requirements for sterility, leak prevention, and high reliability in the space environment. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings of the embodiments will be briefly described below.

[0021] Figure 1 A schematic diagram of the structure of a closed transfer culture device provided by this utility model;

[0022] Figure 2 A schematic diagram of the lower plate provided by this utility model;

[0023] Figure 3 A schematic diagram of the upper plate provided by this utility model;

[0024] Figure 4 A schematic diagram of the lower plate and main controller provided by this utility model;

[0025] Figure 5 A schematic diagram of the internal structure of the culture medium replenishment bottle provided by this utility model;

[0026] In the diagram, 1 is a culture plate; 11 is an upper plate; 111 is a trapezoidal ultrasonic line; 12 is a lower plate; 121 is a mounting groove; 122 is a positioning protrusion; 13 is a culture tank; 14 is a filter; 15 is a main controller; 16 is a vent tube; 17 is a buffer zone; 18 is a culture zone; 19 is a buffer strip; 191 is an outlet; 2 is a culture medium replenishment bottle; 21 is a replenishment tube; 211 is an outer tube; 212 is an inner tube; 22 is a bottle body; 23 is a bottle cap; 231 is a tracheal tube interface; 232 is a liquid tube interface; 233 is an inner interface; 234 is an arc-shaped convex ring; 24 is a tracheal tube; 3 is a cell collection bag; 31 is a first collection tube; 32 is a first collection tracheal tube; 4 is a waste liquid recovery bag; 41 is a second collection tube; 42 is a second collection tracheal tube; 5 is a cell monitoring instrument; 6 is a flow stop clamp; 7 is a needle filter; and 8 is a Luer connector. Detailed Implementation

[0027] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit its scope.

[0028] In the description of this utility model, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", "top", "bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0029] like Figures 1 to 5 As shown, a preferred embodiment of the present invention provides a closed transfer culture device, comprising:

[0030] Culture plate 1, which has a culture chamber inside, and the culture plate 1 is provided with a liquid inlet, a cell liquid outlet and a waste liquid outlet communicating with the culture chamber;

[0031] Culture medium replenishment bottle 2 is used to provide culture medium to the culture chamber, and the culture medium replenishment bottle 2 is connected to the inlet through replenishment tube 21;

[0032] The cell collection bag 3 is connected to the cell outlet via a first collection tube 31, and the cell collection bag 3 is connected to a power device for collecting cells that have completed culture.

[0033] Waste liquid recovery bag 4 is connected to the waste liquid outlet through a second collection pipe 41, and the waste liquid recovery bag 4 is connected to a power unit for collecting waste liquid generated during cultivation.

[0034] In this invention, the culture medium replenishment bottle 2 provides nutrients to the culture chamber in the culture plate 1 through the replenishment tube 21. After the culture is completed, the cells in the culture chamber are sent to the cell collection bag 3 through the power device on the cell collection bag 3 in cooperation with the first collection tube 31. The waste liquid in the culture chamber is sent to the waste liquid collection bag 4 through the power device on the waste liquid collection bag 4 in cooperation with the second collection tube 41. This ensures that the cell culture and recycling process is isolated from the outside world and meets the requirements of sterility, leakage prevention and high reliability in the space environment.

[0035] For example, such as Figure 2 and Figure 3 As shown, the culture plate 1 includes an upper plate 11, a lower plate 12, a filter 14, and a main controller 15. Both the lower plate 12 and the upper plate 11 are provided with culture tanks 13. The lower plate 12 is connected to the upper plate 11, and the culture tanks 13 of the lower plate 12 and the upper plate 11 form the culture chamber. This docking structure simplifies the assembly process and reduces the complexity of space operations. The culture plate 1 has a vent communicating with the culture chamber. The vent is connected to the filter 14 via a vent pipe 16. The filter 14 is a permeable... The gas barrier filter 14 maintains gas exchange while blocking bacteria, ensuring a sterile culture environment. The lower plate 12 is provided with a mounting groove 121 for mounting the main controller 15. The main controller 15 is connected to an external cell monitoring instrument 5 via a data transmission line. The culture plate 1 can be understood as a culture microfluidic chip. In conjunction with the cell monitoring instrument 5, the culture plate 1 is used to detect and transmit data, monitor cell growth at any time, and detect dissolved oxygen, pH value, cell confluence, etc. The data is displayed on the cell monitoring instrument 5 to analyze cell growth.

[0036] For example, the culture chamber is provided with two sets of buffer strips 19 that divide the culture chamber into two buffer zones 17 and a culture zone 18. The culture zone 18 is sandwiched between the two buffer zones 17, and the buffer strips 19 are provided with ports 191 for connecting the culture zone 18 and the buffer zones 17. The liquid inlet and the waste liquid outlet are respectively connected to the two ends of one of the buffer zones 17, and the vent and the cell outlet are respectively connected to the two ends of the other buffer zone 17. In this way, the buffer zones 17 provide buffering during liquid inflow and outflow, preventing excessive impact on the cells cultured in the middle culture zone 18 and effectively protecting the cells.

[0037] Specifically, the replenishment tube 21, the first collection tube 31, and the second collection tube 41 are all equipped with flow stop clamps 6 to precisely control the flow and velocity of the fluid. Through the opening and closing action of the flow stop clamps 6, closed transfer and step-by-step controllable operation (such as during replenishment and cell recovery) can be achieved. During non-operation periods, closing the flow stop clamps 6 can maintain the independence and sealing of each pipeline. During operation, opening and closing in sequence can achieve directional fluid delivery, prevent contamination and leakage, and improve the reliability of the device.

[0038] Furthermore, the lower plate 12 is provided with positioning protrusions 122 at its four corners, and the upper plate 11 is provided with positioning grooves at its four corners that cooperate with the positioning protrusions 122, ensuring precise alignment between the lower plate 12 and the upper plate 11; the edge of the culture tank 13 of the upper plate 11 is provided with trapezoidal ultrasonic lines 111, and the upper plate 11 and the lower plate 12 are ultrasonically connected to ensure that the formed culture chamber is reliably sealed, ensuring that the installation groove 121 is completely isolated from the culture chamber, and that the culture medium cannot enter the position of the main controller 15 and damage the main controller 15.

[0039] For example, such as Figure 4 As shown, the culture medium replenishment bottle 2 includes a bottle body 22 and a bottle cap 23. The bottle cap 23 is fitted onto the bottle opening of the bottle body 22. Specifically, the bottle cap 23 is screwed onto the bottle opening of the bottle body 22. The bottle cap 23 is provided with an air tube 24 communicating with the inner cavity of the bottle body 22. The end of the air tube 24 is provided with a needle filter 7. The needle filter 7 is connected to a syringe. The replenishment tube 21 passes through the bottle cap 23 and extends into the bottom of the bottle body 22. When replenishing the culture chamber of the culture plate 1, the syringe injects air into the bottle body 22 through the needle filter 7 to pressurize it, so that the culture medium in the bottle body 22 enters the culture chamber of the culture plate 1 through the replenishment tube 21, preventing the formation of a vacuum that prevents the transfer of liquid and ensuring aseptic transfer. The needle filter 7 has a built-in PTFE 0.22um hydrophobic membrane.

[0040] Specifically, the upper part of the bottle cap 23 is provided with an air tube interface 231 and a liquid tube interface 232. The air tube 24 is inserted into the air tube interface 231. The bottom of the bottle cap 23 is provided with an inner interface 233 communicating with the liquid tube interface 232. Two arc-shaped protruding rings 234 are spaced apart on the inner wall of the inner interface 233. The replenishment tube 21 includes an outer tube 211 and an inner tube 212. The inner tube 212 is inserted into the inner interface 233. The two arc-shaped protruding rings 234 can lock the inner tube 212, ensuring a firm seal and preventing the inner tube 212 from falling off due to improper installation, thus improving reliability. One end of the outer tube 211 is inserted into the liquid tube interface 232, and the other end of the outer tube 211 is connected to the liquid inlet through a Luer connector 8. Preferably, the inner tube 212 is a Teflon tube, which, due to its biological inertness and smoothness, ensures that the culture medium does not precipitate, adsorb, or leave residue during transportation.

[0041] For example, the power device is a syringe, and the cell collection bag 3 is provided with a first collection air tube 32. The end of the first collection air tube 32 is provided with a needle filter 7 connected to the syringe. By drawing air (negative pressure) through the syringe connected to the cell collection bag 3, a negative pressure is formed inside the cell collection bag 3, and the cells in the culture plate 1 are drawn into the cell collection bag 3 for recycling. The waste liquid recycling bag 4 is provided with a second collection air tube 42. The end of the second collection air tube 42 is provided with a needle filter 7 connected to the syringe. By drawing air (negative pressure) through the syringe connected to the waste liquid recycling bag 4, a negative pressure is formed inside the waste liquid recycling bag 4, and the waste liquid in the culture plate 1 is drawn into the waste liquid recycling bag 4 for recycling. Both the first collection air tube 32 and the second collection air tube 42 are provided with a flow stop clamp 6. Specifically, the first collection air tube 32 is connected to the cell collection bag 3 through a Luer connector 8, and the second collection air tube 42 is connected to the waste liquid recycling bag 4 through a Luer connector 8. The first collection tube 31 is connected to the cell outlet via a Luer connector 8, and the second collection tube 41 is connected to the waste liquid outlet via a Luer connector 8, which facilitates pipe connection and ensures the sealing of the connection.

[0042] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0043] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of the present utility model, and these improvements and substitutions should also be considered within the protection scope of the present utility model.

Claims

1. A closed transfer culture device, characterized in that, include: A culture plate having a culture chamber inside, the culture plate having a liquid inlet, a cell outlet and a waste liquid outlet communicating with the culture chamber; A culture medium replenishment bottle is used to provide culture medium to the culture chamber, and the culture medium replenishment bottle is connected to the inlet via a replenishment tube; A cell collection bag is connected to the cell outlet via a first collection tube, and the cell collection bag is connected to a power device for collecting cells that have completed culture. The waste liquid recovery bag is connected to the waste liquid outlet via a second collection pipe, and the waste liquid recovery bag is connected to a power unit for collecting the waste liquid generated during cultivation.

2. The closed transfer culture device as described in claim 1, characterized in that, The culture plate includes an upper plate, a lower plate, a filter, and a main controller. Both the lower plate and the upper plate are provided with culture tanks. The lower plate is connected to the upper plate, and the culture tanks of the lower plate and the upper plate form the culture chamber. The culture plate is provided with a vent that communicates with the culture chamber. The vent is connected to the filter through a vent pipe. The lower plate is provided with a mounting groove for mounting the main controller. The main controller is connected to an external cell monitoring instrument through a data transmission line.

3. The closed transfer culture device as described in claim 2, characterized in that, The culture chamber is provided with two sets of buffer strips that divide the culture chamber into two buffer zones and a culture zone. The culture zone is sandwiched between the two buffer zones, and the buffer strips are provided with ports for connecting the culture zone and the buffer zones. The liquid inlet and the waste liquid outlet are respectively connected to the two ends of one of the buffer zones, and the vent and the cell outlet are respectively connected to the two ends of the other buffer zone.

4. The closed transfer culture device as described in claim 3, characterized in that, The replenishment tube, the first collection tube, and the second collection tube are all equipped with flow-stopping clamps.

5. The closed transfer culture device as described in claim 2, characterized in that, The lower plate has positioning protrusions at its four corners, and the upper plate has positioning grooves at its four corners that mate with the positioning protrusions. The upper plate has trapezoidal ultrasonic lines along the edge of its culture tank, and the upper plate is ultrasonically connected to the lower plate.

6. The closed transfer culture device as described in claim 1, characterized in that, The culture medium replenishment bottle includes a bottle body and a bottle cap. The bottle cap is fitted onto the bottle mouth of the bottle body. The bottle cap is provided with an air tube that communicates with the inner cavity of the bottle body. The end of the air tube is provided with a needle filter. The needle filter is connected to a syringe. The replenishment tube passes through the bottle cap and extends into the bottom of the bottle body.

7. The closed transfer culture device as described in claim 6, characterized in that, The upper part of the bottle cap is provided with an air tube interface and a liquid tube interface. The air tube is inserted into the air tube interface. The bottom of the bottle cap is provided with an inner interface that communicates with the liquid tube interface. Two arc-shaped convex rings are provided at intervals on the inner wall of the inner interface. The liquid replenishment tube includes an outer tube and an inner tube. The inner tube is inserted into the inner interface. One end of the outer tube is inserted into the liquid tube interface. The other end of the outer tube is connected to the liquid inlet through a Luer connector.

8. The closed transfer culture device as described in claim 7, characterized in that, The inner tube is a Teflon tube.

9. The closed transfer culture device as described in claim 1, characterized in that, The power device is a syringe, the cell collection bag is provided with a first collection tube, the end of the first collection tube is provided with a needle filter connected to the syringe, the waste liquid recovery bag is provided with a second collection tube, the end of the second collection tube is provided with a needle filter connected to the syringe, and both the first collection tube and the second collection tube are provided with flow stop clamps.

10. The closed transfer culture device as described in claim 1, characterized in that, The first collection tube is connected to the cell outlet via a Luer connector, and the second collection tube is connected to the waste liquid outlet via a Luer connector.

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