Layered stem cell incubator

Abstract

The utility model discloses a layered stem cell cultivation device belongs to stem cell cultivation technical field, including the incubator, and the inner chamber of incubator is equipped with a plurality of layered board, and the top surface of each layered board is equipped with the incubation board for placing stem cell culture dish, and the inner chamber of each layered board is all seted up to have the empty slot, and the inner side wall of each empty slot is all connected with two springs, and the free end of every two springs is all commonly connected with the rectangular plate, and the side surface of each rectangular plate is all connected with the clamping block that is penetrated to one side of layered board, and the both sides inner wall of incubator is equally spaced and is equipped with a plurality of clamping groove that is matched with the clamping block, and the side surface of each rectangular plate is all connected with the pull rope, and the free end of each pull rope is all connected with the connecting block that is penetrated to one side of layered board, and the one end of every two connecting blocks that is penetrated to one side of layered board is all commonly connected with the drag board. This layered stem cell cultivation device solves the problem that the internal space is difficult to divide, and reduces the influence on stem cell cultivation.

Description

Technical Field

[0001] This invention belongs to the field of stem cell culture technology, and in particular to a layered stem cell culture device. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated daughter cells. In biopharmaceutical and biological experiments, stem cells are often cultured in incubators. However, current culture devices often use fixed partitions for layering. Since the culture dishes required for cultivation vary in size and space, the fixed partitions are not conducive to dividing the internal storage space, thus hindering the layering of items and affecting stem cell cultivation.

[0003] A Chinese patent (authorization announcement number CN217418665U) discloses a layered stem cell culture device, which "includes an outer box and an inner box. A plurality of first damping columns are fixedly installed on the lower inner surface of the outer box, and a plurality of second damping columns are fixedly installed on both opposite inner surfaces of the outer box. The inner box is fixedly installed on the upper surface of the first damping columns, and the top of the second damping columns is connected to the inner box. The impact on the inner box is reduced by the various first and second damping columns." It is evident that the cited patent document suffers from the problem of inconvenience in dividing the internal space. Utility Model Content

[0004] The purpose of this invention is to provide a layered stem cell culture device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a layered stem cell culture device, comprising a culture box, wherein the inner cavity of the culture box is provided with multiple layered plates, the top surface of each layered plate is provided with a culture plate for placing stem cell culture dishes, the inner cavity of each layered plate is provided with a slot, the inner sidewall of each slot is connected to two springs, the free ends of each pair of springs are connected to a rectangular plate, one side of each rectangular plate is connected to a locking block penetrating to one side of the layered plate, the inner walls of both sides of the culture box are provided with a plurality of locking slots that fit with the locking blocks, one side of each rectangular plate is connected to a pull rope, the free end of each pull rope is connected to a connecting block penetrating to one side of the layered plate, and the ends of each pair of connecting blocks penetrating to one side of the layered plate are connected to a pull plate.

[0006] Preferably, each of the layered plates has a groove on its top surface, and a slide rail is installed on the inner sidewall of each groove.

[0007] Preferably, each of the cultivation plates has a slider connected to its bottom surface, and each slider is slidably sleeved on the periphery of the slide rail.

[0008] Preferably, each of the layered plates has two mounting boxes mounted on its top surface, and each mounting box has a threaded shaft rotatably connected to its inner bottom surface.

[0009] Preferably, each of the threaded shafts is threaded with a limiting block that penetrates the layered plate on its circumference, and each of the cultivation plates has a limiting groove symmetrically opened on its top surface that fits the limiting block.

[0010] Preferably, each of the culture plates has a placement groove on its top surface for placing stem cell culture dishes, and each of the culture plates has multiple through grooves in its inner cavity, with bidirectional threaded rods rotatably connected to the inner cavities of the multiple through grooves.

[0011] Preferably, each of the plurality of bidirectional threaded rods is threaded with a movable block extending through to the top of the culture plate, and one side of each movable block is fitted with an arc-shaped block for clamping the stem cell culture dish.

[0012] Compared with the prior art, the technical effects and advantages of this utility model are as follows: This tiered stem cell culture device uses a linkage structure of a pull plate, a pull rope, and a rectangular plate. Simply pulling the pull plate will cause the pull rope to retract, compressing the spring in the rectangular plate and disengaging the locking block from the slot. At this point, the height of the tiered plate can be freely adjusted. Compared to existing devices that use fixed partitions, this device can divide the internal space according to the size of the stem cell culture dish, which is beneficial for the tiered placement of stem cells.

[0013] The culture plate is able to be moved to an open space through the sliding fit of sliders and rails, which facilitates medium replacement, sampling, and cell observation.

[0014] The two arc-shaped blocks, driven by a bidirectional threaded rod, can move synchronously relative to each other, thereby clamping and fixing the stem cell culture dish placed in the placement tank. Attached Figure Description

[0015] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2This is a side sectional view of the incubator of this utility model; Figure 3 This is a top sectional view of the layered plate of this utility model; Figure 4 This is a side sectional view of the layered plate of this utility model; Figure 5 For the present utility model Figure 4 Enlarged schematic diagram of the structure at point A; Figure 6 This is a side sectional view of the cultivation plate of this utility model.

[0017] Explanation of reference numerals in the attached figures: In the diagram: 1. Incubator; 2. Layered plate; 3. Incubation plate; 4. Empty slot; 5. Spring; 6. Rectangular plate; 7. Locking block; 8. Locking groove; 9. Pull rope; 10. Connecting block; 11. Pull plate; 12. Groove; 13. Slide rail; 14. Slider; 15. Mounting box; 16. Threaded shaft; 17. Limiting block; 18. Limiting groove; 19. Placement groove; 20. Through groove; 21. Two-way threaded rod; 22. Movable block; 23. Arc-shaped block; 24. Box door. Detailed Implementation

[0018] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0019] Unless otherwise defined, the directions mentioned herein, such as up, down, left, right, front, back, inside, and outside, are based on the directions shown in the figures of this utility model, and are explained here together.

[0020] The connection method can be any existing method, such as bonding, welding, or bolting, depending on the actual needs.

[0021] like Figures 1 to 6 The layered stem cell culture device shown includes a culture box 1. One side of the culture box 1 is connected to a glass door 24 via a hinge. The door 24 is fixed to the culture box 1 by a snap-fit ​​connection. In addition to the snap-fit, an inflatable silicone sealing ring (connected to a micro air pump, which inflates and expands after the door is closed, achieving a 100% sealing rate) is added to the contact surface between the door 24 and the culture box 1 to prevent external air from seeping in. The glass of the door 24 is made of double-layered anti-fog tempered glass, with an antibacterial film (such as a silver ion coating) coated on the inside to reduce the growth of microorganisms in condensation.

[0022] A HEPA high-efficiency filter and a one-way exhaust valve are installed at the top of the incubator 1, and a sterile gas inlet is installed at the bottom, forming a one-way airflow from top to bottom. Even if the local seal is briefly broken when the incubation plate 3 is pulled out, the airflow will quickly carry away any contaminants that may have entered, maintaining a positive pressure sterile environment inside the chamber.

[0023] The incubator 1 has multiple layered plates 2 inside. Each layered plate 2 has a culture plate 3 on its top surface for placing stem cell culture dishes. Each layered plate 2 has an inner cavity with a slot 4. Two springs 5 ​​are connected to the inner wall of each slot 4. A rectangular plate 6 is connected to the free end of each pair of springs 5. A locking block 7 is connected to one side of each rectangular plate 6, extending through to one side of the layered plate 2. Several slots 8, which fit the locking blocks 7, are equidistantly spaced on both inner walls of the incubator 1. A pull rope 9 is connected to one side of each rectangular plate 6. A connecting block 10, extending through to one side of each layered plate 2, is connected to the free end of each pull rope 9. A pull plate 11 is connected to the end of each pair of connecting blocks 10 extending through to one side of the layered plate 2. Initially, the locking block 7 is in the inner cavity of the slot 8, fixing the position of the layered plate 2. Then, the pull plate is pulled according to the size of the stem cell culture dish. 11. This will drive the two connecting blocks 10 to pull the pull rope 9, allowing the pull rope 9 to pull the rectangular plate 6 and the locking block 7, and compress the spring 5. The spring 5 will deform under pressure, buffering the force to achieve a damping effect, allowing the locking block 7 to move laterally and move out of the locking slot 8. At this time, the layered plate 2 will move longitudinally to adapt the space between the multiple layered plates 2 to the space required by the stem cell culture dish. After the layered plate 2 moves to the appropriate position, the pull plate 11 will be released, and the rectangular plate 6 and the locking block 7 will return to their original positions through the rebound of the spring 5, so that the locking block 7 can be locked into the locking slot 8, fixing the position of the layered plate 2 after movement. At this time, the stem cell culture dish can be placed in the placement slot 19, avoiding the problem of difficulty in dividing the internal storage space of the device, which is not conducive to the layered placement of stem cells, and reducing the impact on stem cell culture.

[0024] Spring 5 is made of piano wire and has a service life of more than 100,000 cycles.

[0025] A limiting roller is installed on the inner bottom surface of the slot 4. The pull rope 9 passes over the limiting roller, so that the pull rope 9 can be in a 90-degree state. This ensures that when the pull plate 11 pulls the pull rope 9, the pull rope 9 can drive the rectangular plate 6 and the locking block 7 to move laterally. The pull rope 9 and the spring 5 are staggered to avoid mutual interference.

[0026] Limiting rods are symmetrically welded to the inner wall of the slot 4. The rectangular plate 6 is slidably sleeved on the periphery of the two limiting rods, which can limit the movement of the rectangular plate 6. The positions of the two limiting rods, the spring 5, and the pull rope 9 are also staggered to avoid mutual interference.

[0027] The inner wall of the slot 4 is welded with a slide rail, and the connecting block 10 is slidably sleeved on the periphery of the slide rail, which can also limit the movement of the connecting block 10.

[0028] Each layered plate 2 has a groove 12 on its top surface, and a slide rail 13 is installed on the inner side wall of each groove 12. Each culture plate 3 has a slider 14 connected to its bottom surface. Each slider 14 is slidably sleeved on the periphery of the slide rail 13. By pulling the culture plate 3, the slider 14 can be moved around the periphery of the slide rail 13, so that the culture plate 3 can be moved from the inside of the culture box 1 to the outside, so that the culture plate 3 can be in an open space, which is convenient for changing the medium, taking samples, and observing cells.

[0029] An accordion-style dust cover (made of medical-grade PVC, foldable) is installed on the inner wall of the groove 12. One end of the dust cover is connected to the slider 14, and the other end is fixed to the end of the groove 12. When the culture plate 3 is pulled out, the dust cover extends and retracts synchronously, completely covering the slide rail 13 to prevent the slide rail 13 from being exposed to dirt. At the same time, sterile grease (food-grade silicone grease) is embedded in the contact area between the slider 14 and the slide rail 13, which reduces friction and forms a sealing film to block gaps.

[0030] A heating film is embedded in the inner cavity of each layer plate 2. The heating film is not located in the area of ​​the empty slot 4 and is positioned to avoid adjustment structures such as the pull rope 9 and spring 5. The heating film is electrically connected to the temperature control module outside the incubator 1 to ensure that the installation stability of the heating film is not affected when the height of the layer plate 2 is adjusted. At the same time, a temperature sensor is installed on the inner side wall of the groove 12 of the incubator 3. The sensor signal is transmitted to the temperature control module to achieve independent temperature control for each layer (37±0.1℃). This design works in conjunction with the adjustable structure of the layer plate 2 to avoid temperature gradient differences caused by changes in layer height and ensure that culture dishes of different heights are in a consistent temperature environment.

[0031] A gas distributor is installed on the top of the incubator 1, which is connected to the edge of each layer of incubation plate 3 through a pipe and avoids the placement slot 19. The nozzle direction is parallel to the incubation plate 3 to avoid the airflow directly impacting the culture dish. Each layer is equipped with a corresponding CO2 sensor, which is linked to the gas distributor for adjustment. This structure is adapted to the pull-out function of the incubation plate 3. When the incubation plate 3 is pulled out, the gas nozzles of the corresponding layer automatically reduce the flow rate to reduce the loss of gas in the chamber. After the incubation plate 3 is pushed back, normal gas supply is restored to maintain the overall gas environment stability.

[0032] Each layered plate 2 has two mounting boxes 15 installed on its top surface. Each mounting box 15 has a threaded shaft 16 rotatably connected to its inner bottom surface. The top of the threaded shaft 16 passes through the mounting box 15 and is connected to a knob. Each threaded shaft 16 has a threaded limiting block 17 threaded around its periphery, penetrating the layered plate 2. Each cultivation plate 3 has symmetrically formed limiting grooves 18 on its top surface that fit the limiting blocks 17. Initially, the limiting blocks 17 are positioned within the limiting grooves 18, thus fixing the position of the cultivation plate 3. If it is necessary to move the cultivation plate 3 to an open space, the knob can be rotated... Turn the knob to rotate the threaded shaft 16, which in turn moves the limiting block 17 upward, allowing it to be removed from the inner cavity of the limiting groove 18. This releases the fixation on the culture plate 3. At this point, the culture plate 3 can be pulled to an open space. After changing the medium, taking samples, and performing cell operations, return the culture plate 3 to its original position, aligning the limiting groove 18 with the limiting block 17. Then, rotate the knob in the opposite direction, causing the threaded shaft 16 to move the limiting block 17 downward, which then engages with the inner cavity of the limiting groove 18, thus fixing the position of the culture plate 3.

[0033] Each culture plate 3 has a placement groove 19 on its top surface for placing stem cell culture dishes. Each culture plate 3 has multiple through grooves 20 in its inner cavity. The inner cavity of each through groove 20 is rotatably connected to a bidirectional threaded rod 21. One end of each bidirectional threaded rod 21 is connected to a round rod that extends through to one side of the culture plate 3, and the free end of each round rod is also connected to a motor. The periphery of each bidirectional threaded rod 21 is threaded with movable blocks 22 that extend through to the top of the culture plate 3. One side of each movable block 22 is equipped with an arc-shaped block 23 for clamping the stem cell culture dish. After the stem cell culture dish is placed in the inner cavity of the placement groove 19, turning the knob will drive the bidirectional threaded rod 21 to rotate through the round rod, causing the two movable blocks 22 to move in opposite directions, thereby causing the two arc-shaped blocks 23 to move in opposite directions, so that the two arc-shaped blocks 23 can clamp the stem cell culture dish and fix it.

[0034] Multiple placement slots 19, movable blocks 22 and arc-shaped blocks 23 are arranged alternately. The top surface of the cultivation plate 3 is provided with a rectangular slot for the movable block 22 to pass through. A horizontal block is welded to the inner wall of the rectangular slot. The movable block 22 is slidably sleeved on the periphery of the horizontal block, which can limit the movement of the movable block 22 and prevent it from rotating with the bidirectional threaded rod 21.

[0035] Working principle: Before placing the stem cell culture dish in the placement slot 19, the pull plate 11 is pulled according to the size of the culture dish. This pull plate 11 drives the two connecting blocks 10 to pull the pull rope 9, which in turn pulls the rectangular plate 6 and the locking block 7, compressing the spring 5. This allows the locking block 7 to move laterally and move out of the slot 8. At this time, the layered plates 2 are moved longitudinally to adapt the space between the multiple layered plates 2 to the space required by the stem cell culture dish. After the layered plates 2 have moved to the appropriate position, the pull plate 11 is released. The spring 5 rebounds, allowing the rectangular plate 6 and the locking block 7 to return to their original positions, so that the locking block 7 can be locked into the slot 8. This fixes the position of the layered plates 2 after movement. At this point, the stem cell culture dish can be placed in the placement slot 19, avoiding the problem of difficulty in dividing the internal storage space of the device, which is not conducive to the layered placement of stem cells, and reducing the impact on stem cell culture.

[0036] It should be noted that, in this document, relational terms such as "one" and "two" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A layered stem cell culture device, comprising a culture box (1), characterized in that: The inner cavity of the incubation box (1) is provided with multiple layered plates (2). The top surface of each layered plate (2) is provided with a culture plate (3) for placing stem cell culture dishes. The inner cavity of each layered plate (2) is provided with a slot (4). The inner side wall of each slot (4) is connected to two springs (5). The free ends of each two springs (5) are connected to a rectangular plate (6). One side of each rectangular plate (6) is connected to a locking block (7) that penetrates to one side of the layered plate (2). The inner walls of both sides of the incubation box (1) are provided with several slots (8) that fit the locking blocks (7). One side of each rectangular plate (6) is connected to a pull rope (9). The free end of each pull rope (9) is connected to a connecting block (10) that penetrates to one side of the layered plate (2). The ends of each two connecting blocks (10) that penetrate to one side of the layered plate (2) are connected to a pull plate (11).

2. The layered stem cell culture device according to claim 1, characterized in that: Each of the layered plates (2) has a groove (12) on its top surface, and each groove (12) has a slide rail (13) installed on its inner sidewall.

3. The layered stem cell culture device according to claim 2, characterized in that: Each of the cultivation plates (3) has a slider (14) connected to its bottom surface, and each slider (14) is slidably sleeved on the periphery of the slide rail (13).

4. The layered stem cell culture device according to claim 1, characterized in that: Each of the layered plates (2) has two mounting boxes (15) mounted on its top surface, and each mounting box (15) has a threaded shaft (16) rotatably connected to its inner bottom surface.

5. A layered stem cell culture device according to claim 4, characterized in that: Each of the threaded shafts (16) is threaded with a limiting block (17) that penetrates the layered plate (2) on its periphery, and each of the cultivation plates (3) has a limiting groove (18) symmetrically opened on its top surface that fits the limiting block (17).

6. The layered stem cell culture device according to claim 1, characterized in that: Each of the culture plates (3) has a placement groove (19) on its top surface for placing stem cell culture dishes, and each of the culture plates (3) has multiple through grooves (20) in its inner cavity, and the inner cavity of each of the multiple through grooves (20) is rotatably connected to a bidirectional threaded rod (21).

7. A layered stem cell culture device according to claim 6, characterized in that: Each of the multiple bidirectional threaded rods (21) has a movable block (22) threaded around its periphery, extending to the top of the culture plate (3). Each movable block (22) has an arc-shaped block (23) installed on one side for clamping the stem cell culture dish.

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