A cell enrichment staining integrated device

Abstract

The utility model discloses a cell enrichment dyeing integrated equipment, including, base, enrichment component sets up on the base for enrichment target cell, dyeing component sets up on the base for dyeing target cell, liquid adding component, including setting first guide rail and first liquid adding pipe and second liquid adding pipe of the sliding cooperation of first guide rail on the base, and first liquid adding pipe is used to add first reagent to enrichment component, and second liquid adding pipe is used to add second reagent to dyeing component. The utility model discloses through with enrichment component and dyeing component integration on the same base, and is equipped with the liquid adding component who has the first liquid adding pipe and second liquid adding pipe of the first guide rail sliding, and the guide rail design of liquid adding component has guaranteed the accuracy and repeatability of liquid adding, has realized the mechanization operation of reagent addition, has reduced the time and step of manual reagent taking and placing significantly, thereby has improved the efficiency of entire cell enrichment, dyeing and subsequent identification process effectively.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical devices, specifically, it relates to an integrated device for cell enrichment and staining. Background Technology

[0002] The pharmaceutical industry and the biomedical engineering industry are the two pillars of the modern pharmaceutical industry. The biomedical industry is composed of the biotechnology industry and the pharmaceutical industry. Biomedical engineering is a general term for the comprehensive application of the principles and methods of life science and engineering science to understand the structure, function and other life phenomena of the human body at multiple levels from the perspective of engineering, including molecules, cells, tissues, organs and even the entire human body system. It studies artificial materials, products, devices and systems technologies used for disease prevention, treatment, human function assistance and health care.

[0003] In recent years, several emerging tumor diagnostic and detection technologies have emerged in the field of biomedical engineering, such as circulating tumor DNA (ctDNA) and circulating tumor cell (CTC) detection methods, known as liquid biopsy. CTC detection offers convenient sample collection, overcoming the drawbacks of inconvenient sample collection and patient-invasive procedures associated with histopathological examination. Studies have shown that CTCs can be detected in peripheral blood before solid tumors form, making CTC detection highly suitable for early screening and diagnosis of malignant tumors. Furthermore, CTC detection demonstrates excellent efficacy in assessing prognosis, monitoring disease progression, predicting recurrence, monitoring postoperative microlesions in malignant tumors, and designing and monitoring the effectiveness of targeted drug therapy. It is currently an advanced method for early screening and diagnosis of malignant tumors. Because the concentration of CTCs in peripheral blood is very low, CTC detection requires prior enrichment of CTCs before testing.

[0004] The testing process involves steps such as enrichment and staining of the collected body fluids. Currently, reagents on the market are handled manually, which significantly reduces the efficiency of reagent identification.

[0005] In view of this, this utility model is proposed. Utility Model Content

[0006] In order to solve at least some of the problems mentioned above, the present invention aims to provide a cell enrichment and staining integrated device that can improve reagent identification efficiency.

[0007] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is as follows:

[0008] A cell enrichment and staining integrated device, comprising,

[0009] Base;

[0010] Enrichment components, mounted on the base, are used to enrich target cells;

[0011] The staining assembly, mounted on the base, is used to stain target cells;

[0012] The liquid addition assembly includes a first guide rail disposed on a base and a first liquid addition tube and a second liquid addition tube that slide in cooperation with the first guide rail. The first liquid addition tube is used to add a first reagent to the enrichment assembly, and the second liquid addition tube is used to add a second reagent to the staining assembly.

[0013] In some embodiments, the first guide rail extends in a first horizontal direction, and multiple enrichment components and staining components are arranged along the first horizontal direction. The first liquid addition tube and the second liquid addition tube are movably arranged on the first guide rail along the first horizontal direction. The enrichment components, staining components and the first guide rail are arranged at intervals in a second horizontal direction perpendicular to the first horizontal direction.

[0014] In some embodiments, the liquid dispensing assembly further includes:

[0015] A first lead screw and a first motor that drives the first lead screw, a first slider is mounted on the first lead screw, the first slider slides along a first horizontal direction and engages with a first guide rail, and the first liquid filling pipe and the second liquid filling pipe are connected to the first slider.

[0016] In some embodiments, the liquid dispensing assembly further includes:

[0017] The first reagent bottle is used to store the first reagent;

[0018] The first injection pump is connected between the first reagent bottle and the first injection tube via a pipeline, and is used to pump the first reagent into the first injection tube.

[0019] The second reagent bottle is used to store the second reagent;

[0020] The second injection pump is connected between the second reagent bottle and the second injection tube via a pipeline, and is used to pump the second reagent into the second injection tube.

[0021] In some implementations, the enrichment component includes:

[0022] A connecting seat is mounted on the base and has a through channel running vertically through the base.

[0023] The filter contains a filter membrane. The bottom of the filter is detachably connected to the top of the connector and communicates with the channel. The first injection tube adds the first reagent to the filter through the opening at the top of the filter.

[0024] The filtrate negative pressure pump is connected to the bottom of the connector and the channel via a pipeline. It is used to provide negative pressure and draw waste liquid from the filter.

[0025] In some embodiments, the top of the connector is provided with a sealing suction cup communicating with the channel, the outer wall of the connector protrudes circumferentially to form a sealing ring, and the bottom of the filter is fitted onto the top of the connector and is interference-fitted with the sealing ring.

[0026] Further, the staining assembly includes:

[0027] The staining tank has an opening at the top for adding the second reagent via a second dispensing tube;

[0028] A collection tank is located on the side of the dyeing tank and is connected to the dyeing tank. The bottom wall of the dyeing tank slopes and transitions to the collection tank.

[0029] In some embodiments, the base is provided with a bracket, the first guide rail is disposed on the bracket, and the first liquid addition tube and the second liquid addition tube are suspended above the enrichment component and the staining component.

[0030] Furthermore, the base is equipped with a waste liquid suction assembly, including,

[0031] The second guide rail and the pipette assembly that slides on the second guide rail are used to aspirate waste liquid from the dyeing assembly.

[0032] The suspension has a second lead screw extending vertically and a second motor driving the second lead screw. The second guide rail is set vertically on the suspension and slidably connected to a second slider. The second slider is connected to the second lead screw. The suction tube assembly is connected to the second slider and located above the dyeing assembly.

[0033] The waste liquid negative pressure pump is connected to the suction tube assembly through pipelines to provide negative pressure and suction the waste liquid in the dyeing assembly.

[0034] Furthermore, the straw assembly includes:

[0035] The connecting rod is horizontally connected to the second slider;

[0036] Multiple suction tubes are arranged on the connecting rod along the first horizontal direction, with the suction end of the suction tube facing the staining assembly.

[0037] By adopting the above technical solution, this utility model has the following beneficial effects compared with the prior art.

[0038] This invention integrates the enrichment component and the staining component on the same base and is equipped with a liquid addition component having a first liquid addition tube and a second liquid addition tube that can slide along the guide rail. The guide rail design of the liquid addition component ensures the accuracy and repeatability of liquid addition, realizes the mechanization of reagent addition, significantly reduces the time and steps of manual reagent handling, and thus effectively improves the efficiency of the entire cell enrichment, staining and subsequent identification process.

[0039] The enrichment component, staining component, and first guide rail are arranged at intervals along the second horizontal direction, so that the movement path of the liquid addition component is orthogonal to the arrangement direction of the functional components. This layout optimizes the use of the equipment's planar space, shortens the movement distance of the liquid addition tube, avoids interference between components, and improves operating efficiency.

[0040] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0041] The accompanying drawings, as part of this utility model, are used to provide a further understanding of the present utility model. The illustrative embodiments and descriptions of the present utility model are used to explain the present utility model, but do not constitute an undue limitation of the present utility model. Obviously, the drawings described below are merely some embodiments; those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0042] Figure 1 This is a perspective view of the cell enrichment and staining integrated device provided in the embodiments of this application;

[0043] Figure 2 This is a front view of the cell enrichment and staining integrated device provided in the embodiments of this application;

[0044] Figure 3 This is a left view of the cell enrichment and staining integrated device provided in the embodiments of this application;

[0045] Figure 4 This is a top view of the cell enrichment and staining integrated device provided in the embodiments of this application;

[0046] Figure 5 This is a schematic diagram of the liquid addition assembly provided in the embodiments of this application;

[0047] Figure 6 yes Figure 5 Sectional view in the BB direction;

[0048] Figure 7 This is a schematic diagram of the structure of the enrichment component provided in the embodiments of this application;

[0049] Figure 8 yes Figure 7 Sectional view along the AA direction;

[0050] Figure 9 This is a schematic diagram of the structure of the dyeing component provided in the embodiments of this application;

[0051] Figure 10 This is a schematic diagram of the waste liquid suction assembly provided in the embodiments of this application.

[0052] In the picture:

[0053] 1. Base; 11. Stand;

[0054] 2. First guide rail; 21. First liquid filling pipe; 22. Second liquid filling pipe; 23. First lead screw; 24. First motor; 25. First slider; 26. First fixing block; 27. Second fixing block;

[0055] 3. Connecting seat; 31. Channel; 32. Filter; 33. Sealing ring;

[0056] 4. Dyeing tank; 41. Collection tank;

[0057] 5. Second guide rail; 51. Suspension; 52. Second lead screw; 53. Second motor; 54. Second slider; 55. Connecting rod; 56. Suction tube;

[0058] 6. Workbench.

[0059] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0060] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0061] In the description of this utility model, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0062] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0063] To improve the user experience when using the integrated cell enrichment and staining device, please refer to... Figures 1 to 6This application provides a cell enrichment and staining integrated device, including a base 1, a liquid addition component, and an enrichment component and a staining component disposed on the base 1. The enrichment component is used to enrich target cells, and the staining component is used to stain target cells. The liquid addition component includes a first guide rail 2 disposed on the base 1 and a first liquid addition tube 21 and a second liquid addition tube 22 that slide and cooperate with the first guide rail 2. The first liquid addition tube 21 is used to add a first reagent to the enrichment component, and the second liquid addition tube 22 is used to add a second reagent to the staining component.

[0064] Furthermore, the base 1 serves as the basic support structure for the entire device, providing an installation platform and stability for other functional components. The enrichment component is used to receive body fluid samples containing target cells and perform the operation of enriching the target cells. The staining component is adjacent to the enrichment component or located in a preset position. The staining component is used to receive the enriched target cells and perform the staining operation on the target cells.

[0065] The first guide rail 2 in the liquid addition assembly is fixedly installed on the base 1. The first liquid addition tube 21 is slidably connected to the first guide rail 2 and is used to hold and precisely add the first reagent required to complete the enrichment process, such as lysis buffer, buffer solution, etc., to the enrichment assembly. The second liquid addition tube 22 is also slidably connected to the first guide rail 2 and is used to hold and precisely add the second reagent required to complete the staining process, such as dye, fixative, permeation solution, etc., to the staining assembly.

[0066] The equipment's workflow is as follows:

[0067] First, the body fluid sample is placed in the enrichment component; then, the first liquid addition tube 21 slides along the first guide rail 2 to a predetermined position above the enrichment component, and the first reagent is added to it, initiating and completing the enrichment process of the target cells; the enriched target cells are transferred to the staining component; the second liquid addition tube 22 slides along the first guide rail 2 to a predetermined position above the staining component, and the second reagent is added to it, initiating and completing the staining process of the target cells; finally, the stained cell sample can be used for subsequent identification and analysis.

[0068] This invention integrates the enrichment component and the staining component on the same base 1, and is equipped with a liquid addition component having a first liquid addition tube 21 and a second liquid addition tube 22 that can slide along the guide rail. The guide rail design of the liquid addition component ensures the accuracy and repeatability of liquid addition, realizes the mechanization of reagent addition, significantly reduces the time and steps of manual reagent handling, and thus effectively improves the efficiency of the entire cell enrichment, staining and subsequent identification process.

[0069] In some implementations, see Figures 1 to 4Multiple enrichment components and staining components are arranged along the first horizontal direction. The first liquid addition tube 21 and the second liquid addition tube 22 are movably arranged on the first guide rail 2 along the first horizontal direction. The enrichment components, staining components and the first guide rail 2 are arranged at intervals in the second horizontal direction perpendicular to the first horizontal direction.

[0070] Understandably, spatial coordinate axes include the X-axis, Y-axis, and Z-axis, which are perpendicular to each other. The first horizontal direction can be the X-axis direction, and the second horizontal direction can be the Y-axis direction.

[0071] During the addition of reagents, the process proceeds in the order of enrichment followed by staining. Specifically, the enrichment process is performed first, with the first reagent addition tube 21 moving along the first guide rail 2 in the first horizontal direction (X-axis direction) and stopping sequentially above the target enrichment components, precisely adding the first reagent to each enrichment component. After enrichment is completed, the second reagent addition tube 22 is then moved along the same first guide rail 2 in the first horizontal direction and stopping sequentially above the target staining components, precisely adding the second reagent to each staining component to achieve cell staining.

[0072] Since the enrichment components, staining components, and the first guide rail 2 are arranged at intervals in the second horizontal direction (Y-axis direction), multiple enrichment components can perform cell enrichment operations simultaneously, and multiple staining components can perform cell staining operations simultaneously, significantly increasing the sample processing capacity per unit time.

[0073] Furthermore, the enrichment components, staining components, and the first guide rail 2 are arranged at intervals along the Y-axis, making the movement path of the liquid addition components orthogonal to the arrangement direction of the functional components. This layout optimizes the use of the equipment's planar space, shortens the movement distance of the liquid addition tube, avoids interference between components, and improves operating efficiency. At the same time, the liquid addition operation of the first liquid addition tube 21 to multiple enrichment components and the second liquid addition tube 22 to multiple staining components is realized through a single first guide rail 2, reducing the complexity and cost of multi-track design while ensuring liquid addition accuracy and reliability.

[0074] In some implementations, see Figures 1 to 6 The liquid filling assembly also includes: a first lead screw 23 and a first motor 24 that drives the first lead screw 23. A first slider 25 is mounted on the first lead screw 23. The first slider 25 slides along the first horizontal direction and is engaged with the first guide rail 5. The first liquid filling tube 21 and the second liquid filling tube 22 are connected to the first slider.

[0075] Specifically, the first lead screw 23 is rotatably mounted on the base 1, and its axis is parallel to the extension direction (first horizontal direction) of the first guide rail 2. The first motor 24 is fixed to the base 1, and its output shaft is connected to the first lead screw 23 to drive the first lead screw 23 to rotate. The first slider 25 is mounted on the first guide rail 2 and slides in cooperation with the first guide rail 2. The first slider 25 engages with the first lead screw 23 through a threaded hole. The first liquid filling pipe 21 and the second liquid filling pipe 22 are fixedly mounted on the first slider 25 and are driven by the first slider 25 to move along the first guide rail 2.

[0076] In use, the first motor 24 drives the first lead screw 23 to rotate, which in turn moves the first slider 25, the first liquid filling tube 21, and the second liquid filling tube 22 along the first guide rail 2 in the first horizontal direction for positioning.

[0077] By equipping the first liquid filling pipe 21 and the second liquid filling pipe 22 with lead screws, motors and sliders, the two liquid filling pipes can slide on a single guide rail. The moving speed and position can be controlled according to process requirements, which significantly shortens the overall processing cycle.

[0078] Furthermore, the first liquid addition tube 21 is fixed to the first slider 25 via the first fixing block 26, and the second liquid addition tube 22 is fixed to the first slider 25 via the second fixing block 27, for injecting reagents for the dyeing process into the dyeing tank 4. The length of the first fixing block 26 is shorter than the length of the second fixing block 27. In this embodiment, the first fixing block 26 and the second fixing block 27 are arranged in parallel, and their cross-sections are rectangular. Both the upper and lower surfaces of the rectangles are provided with through holes for the liquid addition tubes to pass through, and the liquid addition tubes pass through the through holes and are fixed to the fixing blocks. A limit switch is provided below the first lead screw 23 to control the movement distance of the first slider 25 on the lead screw.

[0079] In some embodiments, the liquid dispensing assembly further includes: a first reagent bottle (not shown) for storing a first reagent; a first dispensing pump (not shown) connected between the first reagent bottle and the first dispensing tube 21 via a pipeline for pumping the first reagent into the first dispensing tube 21; a second reagent bottle (not shown) for storing a second reagent; and a second dispensing pump (not shown) connected between the second reagent bottle and the second dispensing tube 22 via a pipeline for pumping the second reagent into the second dispensing tube 22.

[0080] When the first liquid addition tube 21 moves above the target enrichment component, the first liquid injection pump is activated, injecting the reagent in the first reagent bottle into the enrichment component through the first liquid addition tube 21; when the second liquid addition tube 22 moves above the target staining component, the second liquid injection pump is activated, injecting the reagent in the second reagent bottle into the staining component through the second liquid addition tube 22.

[0081] By integrating the first and second reagent bottles and their corresponding dispensing pumps, a fully automated closed-loop operation from reagent storage to dispensing is achieved, completely eliminating the manual reagent handling process and preventing the risk of contamination. The independent operation of the first and second dispensing pumps ensures that the dispensing volume of the first and second reagents is precisely controllable, avoiding manual operation errors and improving the consistency of enrichment and staining results.

[0082] Understandably, although the first reagent bottle, the first injection pump, the second reagent bottle, and the second injection pump are not shown in the accompanying drawings, this does not affect the specific implementation of this embodiment. Those skilled in the art can adjust and set the above technical features according to the recorded content and actual needs.

[0083] In some implementations, see Figures 1 to 4 , Figure 7 and Figure 8 The enrichment components include a connector 3, a filter 32, and a filtrate negative pressure pump (not shown in the figure).

[0084] The connecting seat 3 is mounted on the base 1 and has a channel 31 that runs vertically through the filter 32. The filter membrane is placed inside the filter 32. The bottom of the filter 32 is detachably connected to the top of the connecting seat 3 and communicates with the channel 31. The first injection tube adds the first reagent to the filter 32 through the opening at the top of the filter 32. The filtrate negative pressure pump is connected to the bottom of the connecting seat 3 and communicates with the channel 31 through a pipeline. It is used to provide negative pressure and draw out the waste liquid in the filter 32.

[0085] Furthermore, the top of the connecting seat 3 is provided with a sealing ring 33, which is used to fasten the connection between the filter 32 and the connecting seat 3, and to achieve a seal when the bottom of the filter 32 is connected to the connecting seat 3.

[0086] Furthermore, the top of the connecting seat 3 is provided with a rubber suction cup that connects to the channel 31. The outer wall of the connecting seat 3 protrudes circumferentially to form a sealing ring 33. The bottom of the filter 32 is fitted onto the top of the connecting seat 3 and is press-fitted with the sealing ring 33 to achieve compression.

[0087] After the filter 32 is installed in the connector 3, the first liquid addition tube 21 adds the first reagent to the filter 32 through the opening at the top of the filter 32. Of course, the first liquid addition tube 21 can also be used to add samples containing cells. The filtrate negative pressure pump is started, and the waste liquid is filtered through the filter membrane and then pumped out from the channel 31. The target cells are retained on the filter membrane to complete the enrichment.

[0088] The filter 32 in this embodiment is designed to be detachable, which facilitates quick replacement of the filter membrane or cleaning. Combined with negative pressure suction, it accelerates liquid filtration and significantly improves cell enrichment efficiency.

[0089] The sealing ring 33 or rubber suction cup structure ensures a reliable seal between the filter 32 and the connecting seat 3, preventing negative pressure failure or waste liquid leakage. The integrated sealing ring 33 achieves self-tightening sealing through interference fit, simplifying assembly steps and reducing maintenance costs.

[0090] It should be noted that the structure of filter 32 can be referenced from the filter components in publication number CN220590059U.

[0091] In some implementations, see Figures 1 to 4 ,and Figure 9 The staining assembly is equipped with a staining tank 4 and a collection tank 41. The top of the staining tank 4 is provided with an opening for the second liquid addition tube 22 to add the second reagent. The bottom wall of the staining tank 4 is provided with a tray for holding the filter membrane (i.e., the cell-containing filter membrane transferred here after enrichment). The collection tank 41 is located on the side of the staining tank 4 and communicates with the staining tank 4. The bottom wall of the staining tank 4 slopes to transition to the collection tank 41.

[0092] The enriched filter membrane is placed on the tray of the staining tank 4. The second liquid addition tube 22 adds the second reagent to the staining tank 4 through the tank opening to wet the filter membrane for staining. After the staining is completed, the waste liquid flows into the collection tank 41 along the inclined bottom wall.

[0093] The inclined bottom wall drives the waste liquid to flow automatically to the side collection tank 41. The collection tank 41 is set independently of the side of the dyeing tank 4, realizing the physical isolation between the waste liquid and the dyeing operation area, and preventing the waste liquid from stagnating and contaminating the filter membrane.

[0094] In some implementations, see Figures 1 to 4 The base 1 is provided with a bracket 11, the first guide rail 2 is set on the bracket 11, and the first liquid addition tube 21 and the second liquid addition tube 22 are suspended above the enrichment component and the staining component.

[0095] During the liquid addition operation, the cell enrichment process is carried out first. The first liquid addition tube 21 moves along the suspended first guide rail 2 to the opening of the filter 32 of the enrichment component to add liquid. Then, the cell staining process is carried out. The second liquid addition tube 22 moves along the same guide rail to the opening of the staining tank 4 of the staining component to add liquid.

[0096] The suspended layout allows for vertical separation of the liquid filling pipe from the functional components on the base 1, avoiding interference between the pipes or mechanical structures and the equipment below. The liquid filling components and the working area of ​​the base 1 are layered vertically, providing unobstructed operating space for replacing the filter 32, cleaning the sealing ring 33, or cleaning the collection tank 41.

[0097] In some embodiments, the first fixing block 26 is provided with a plurality of first liquid addition tubes, each of which is connected to a different first reagent bottle through a pipe; the second fixing block 27 is provided with a plurality of second liquid addition tubes, each of which is connected to a different second reagent bottle through a pipe.

[0098] In other words, different first-stage dosing tubes are used to add different reagents to the filter, and different second-stage dosing tubes are used to add different reagents to the staining tank. During the enrichment process, each first-stage dosing tube injects different reagents into the filter in batches to complete the cell enrichment. Similarly, after the enrichment process is completed, during the staining process, each second-stage dosing tube injects different reagents into the staining tank in batches to complete the cell staining.

[0099] In some implementations, see Figures 1 to 4 , Figure 10 The base 1 is equipped with a waste liquid suction assembly, including a second guide rail 5 and a suction tube assembly that slides on the second guide rail 5. The suction tube assembly is used to suction the waste liquid in the dyeing assembly.

[0100] After staining is completed, the pipette assembly slides along the second guide rail 5 to the target collection tank 41 to perform waste liquid suction operation.

[0101] In some implementations, see Figure 10 The waste liquid suction assembly also includes a suspension 51 and a waste liquid negative pressure pump (not shown in the figure).

[0102] The suspension 51 is provided with a second lead screw 52 extending in the vertical direction (equivalent to the Z-axis direction in the spatial coordinate system) and a second motor 53 driving the second lead screw 52. The second guide rail 5 is arranged in the vertical direction on the suspension 51 and is slidably connected to a second slider 54. The second slider 54 is connected to the second lead screw 52. The suction tube assembly is connected to the second slider 54 and is located above the dyeing assembly. The waste liquid negative pressure pump is connected to the suction tube assembly through a pipeline to provide negative pressure and suck up the waste liquid in the dyeing assembly.

[0103] The output shaft of the second motor 53 is connected to the second lead screw 52 to drive its rotation. The second slider 54 is slidably engaged with the second guide rail 5. The straw assembly is fixedly installed on the second slider 54 and is driven by the second slider 54 to make vertical lifting and lowering movements along the second guide rail 5. The initial position is suspended above the dyeing assembly.

[0104] When pumping waste liquid, the suction tube assembly first descends along the second guide rail 5 to a predetermined depth in the collection tank 41, the waste liquid negative pressure pump is started, and the waste liquid is pumped out through the suction tube assembly. After completion, the second motor 53 drives the suction tube assembly to lift and reset.

[0105] The precise transmission mechanism of the second lead screw 52 and the slider enables vertical positioning control of the suction tube assembly, ensuring the optimal distance between the suction tube opening and the liquid surface of the collection tank 41, avoiding collision with the filter membrane or suction residue. The suspension 51 integrates a vertical drive unit and a second guide rail 5, saving horizontal space. The waste liquid negative pressure pump is independently pressurized, avoiding interference with the negative pressure system of the enrichment component.

[0106] In some implementations, see Figure 10 The pipette assembly includes a connecting rod 55 and a suction tube 56. The connecting rod 55 is horizontally connected to the second slider 54 and extends in a direction parallel to the first horizontal direction. Multiple suction tubes 56 are arranged on the connecting rod 55 along the first horizontal direction. The suction end of the suction tube 56 faces the staining assembly. Furthermore, the suction end of each suction tube 56 is vertically downward toward the collection tank 41 of the staining assembly.

[0107] When the waste liquid is being pumped out, the second slider 54 drives the connecting rod 55 and all the suction pipes 56 to rise and fall synchronously. When the suction pipes 56 descend into the collection tank 41, the waste liquid negative pressure pump starts synchronously, and the waste liquid in multiple collection tanks 41 is pumped out in parallel.

[0108] The design of multiple suction tubes 56 arranged along the first horizontal direction allows them to simultaneously cover and clean the collection tanks 41 of multiple dyeing components, reducing the waste liquid removal time of multiple stations to the time of a single station, thus improving efficiency. Only the second lead screw 52 is needed to achieve coordinated operation of multiple suction tubes 56, significantly reducing the complexity and cost of the control system.

[0109] In some embodiments, the base 1 is mounted on the workbench 6 via a frame, and there is a storage space between the base 1 and the workbench 6. The waste liquid negative pressure pump is installed in the storage space, further realizing the vertical layering of the work area and saving horizontal space.

[0110] Furthermore, the integrated cell enrichment and staining device also includes a waste liquid collection bottle (not shown in the figure) for storing waste liquid generated by the enrichment and staining-related pipelines, facilitating centralized collection and treatment of the waste liquid.

[0111] It should be noted that the pipeline used to transport liquid can be a flexible hose (not shown in the figure), which can be deformably connected between the components to facilitate relative movement between the components.

[0112] In some embodiments, a method for performing cell enrichment staining using the above-described integrated cell enrichment staining device is provided, comprising:

[0113] Step 1: The body fluid sample is placed in the filter 32, and the first liquid addition tube 21 is moved along the first guide rail 2 to above the opening of the target filter 32;

[0114] Step 2: The first injection pump is started, and the first injection tube 21 injects the first reagent into the filter 32;

[0115] Step 3: The filtrate negative pressure pump is started, and the waste liquid is discharged through channel 31 after being filtered through the filter membrane, while the target cells are enriched on the filter membrane;

[0116] Step 4: The enriched filter membrane is manually transferred to the tray of the staining tank;

[0117] Step 5: The second liquid addition pipe 22 moves along the first guide rail 2 to the top of the staining tank 4, the second liquid injection pump is started, and the second reagent is injected into the staining tank 4 to wet the filter membrane. The staining waste liquid flows along the inclined bottom wall and into the collection tank 41 through the liquid collection channel.

[0118] Step 6: The pipette assembly moves along the second guide rail 5 to above the target collection tank 41, and the second motor 53 drives the pipette 56 to descend to a predetermined depth in the collection tank 41;

[0119] Step 7: The waste liquid negative pressure pump starts, and the waste liquid is drawn out through the suction pipe 56. The suction pipe 56 is then raised and reset.

[0120] Step 8: After the enrichment and staining process is completed, the waste liquid generated from cleaning the pipelines related to enrichment and staining is discharged into the waste liquid collection bottle for centralized collection and treatment.

[0121] Furthermore, in step 2, multiple first liquid addition tubes 21 are provided, and each first liquid addition tube injects different reagents into the filter in batches to complete the enrichment of cells; in step 5, multiple second liquid addition tubes are provided, and each second liquid addition tube injects different reagents into the staining tank in batches to complete the staining of cells.

[0122] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to preferred embodiments, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-mentioned technical content to create equivalent embodiments without departing from the scope of the present utility model. The implementation schemes in the above embodiments can also be further combined or replaced. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A cell enrichment and staining integrated device, characterized in that, include, Base; Enrichment components, mounted on the base, are used to enrich target cells; The staining assembly, mounted on the base, is used to stain target cells; The liquid addition assembly includes a first guide rail disposed on a base and a first liquid addition tube and a second liquid addition tube that slide in cooperation with the first guide rail. The first liquid addition tube is used to add a first reagent to the enrichment assembly, and the second liquid addition tube is used to add a second reagent to the staining assembly.

2. The cell enrichment and staining integrated device according to claim 1, characterized in that, The first guide rail extends in a first horizontal direction. Multiple enrichment components and staining components are arranged along the first horizontal direction. The first liquid addition tube and the second liquid addition tube are movably arranged on the first guide rail along the first horizontal direction. The enrichment components, staining components and the first guide rail are arranged at intervals in a second horizontal direction perpendicular to the first horizontal direction.

3. The cell enrichment and staining integrated device according to claim 1, characterized in that, The liquid dispensing assembly also includes: A first lead screw and a first motor that drives the first lead screw, a first slider is mounted on the first lead screw, the first slider slides along a first horizontal direction and engages with a first guide rail, and the first liquid filling pipe and the second liquid filling pipe are connected to the first slider.

4. The cell enrichment and staining integrated device according to claim 1, characterized in that, The liquid dispensing assembly also includes: The first reagent bottle is used to store the first reagent; The first injection pump is connected between the first reagent bottle and the first injection tube via a pipeline, and is used to pump the first reagent into the first injection tube. The second reagent bottle is used to store the second reagent; The second injection pump is connected between the second reagent bottle and the second injection tube via a pipeline, and is used to pump the second reagent into the second injection tube.

5. The cell enrichment and staining integrated device according to any one of claims 1-4, characterized in that, The enrichment component includes: A connecting seat is mounted on the base and has a through channel running vertically through the base. The filter contains a filter membrane. The bottom of the filter is detachably connected to the top of the connector and communicates with the channel. The first liquid addition tube adds the first reagent to the filter through the opening at the top of the filter. The filtrate negative pressure pump is connected to the bottom of the connector and the channel via a pipeline. It is used to provide negative pressure and draw waste liquid from the filter.

6. The cell enrichment and staining integrated device according to claim 5, characterized in that, The top of the connector is provided with a sealing suction cup that communicates with the channel. The outer wall of the connector protrudes circumferentially to form a sealing ring. The bottom of the filter is fitted onto the top of the connector and is press-fitted with the sealing ring.

7. The cell enrichment and staining integrated device according to any one of claims 1-4, characterized in that, The staining assembly includes: The staining tank has an opening at the top for adding the second reagent via a second dispensing tube; A collection tank is located on the side of the dyeing tank and is connected to the dyeing tank. The bottom wall of the dyeing tank slopes and transitions to the collection tank.

8. The cell enrichment and staining integrated device according to any one of claims 1-4, characterized in that, The base is provided with a bracket, the first guide rail is mounted on the bracket, and the first liquid addition tube and the second liquid addition tube are suspended above the enrichment component and the staining component.

9. The cell enrichment and staining integrated device according to any one of claims 1-4, characterized in that, The base is equipped with a waste liquid suction component. include, The second guide rail and the pipette assembly that slides on the second guide rail are used to aspirate waste liquid from the dyeing assembly. The suspension has a second lead screw extending vertically and a second motor driving the second lead screw. The second guide rail is set vertically on the suspension and slidably connected to a second slider. The second slider is connected to the second lead screw. The suction tube assembly is connected to the second slider and located above the dyeing assembly. The waste liquid negative pressure pump is connected to the suction tube assembly through pipelines to provide negative pressure and suction the waste liquid in the dyeing assembly.

10. The cell enrichment and staining integrated device according to claim 9, characterized in that, The straw assembly includes: The connecting rod is horizontally connected to the second slider; Multiple suction tubes are arranged on the connecting rod along the first horizontal direction, with the suction end of the suction tube facing the staining assembly.

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