A device for rapid detection and separation of pathogenic bacteria of polygonatum sibiricum

Abstract

The utility model relates to the field of polygonatum sibiricum pathogenic bacteria detection and separation, specifically a kind of polygonatum sibiricum pathogenic bacteria rapid detection separation device, including detection box;Detection box includes preprocessing bin and detection bin, and hinge connection has upper bin cover on preprocessing bin, and ultrasonic treatment mechanism and grinding filter mechanism are provided in preprocessing bin, and ultrasonic treatment mechanism includes cleaning tank, and the bottom inboard of cleaning tank is provided with ultrasonic generator, and bin door is hingedly connected on detection bin, and the inner side edge of bin door is provided with silica gel sealant strip, and detection bin is provided with polygonatum sibiricum pathogenic bacteria detection mechanism cleaning tank, and water level scale line is fixedly arranged on the inner wall of cleaning tank, and the lower part of the side wall of cleaning tank is fixedly provided with drain pipe, and the outer end of drain pipe is provided with valve body through preprocessing bin, can integrate polygonatum sibiricum cleaning, grinding separation bacteria liquid, pathogenic bacteria detection in detection box, realize polygonatum sibiricum rapid detection.

Description

Technical Field

[0001] This utility model relates to the field of detection and isolation of pathogens of Polygonatum sibiricum, specifically a rapid detection and isolation device for pathogens of Polygonatum sibiricum. Background Technology

[0002] Polygonatum, a perennial herb belonging to the genus Polygonatum in the Liliaceae family, occupies an important position in traditional Chinese medicine. Its dried rhizome is a commonly used medicinal herb with effects such as tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. It has significant therapeutic effects on various symptoms including spleen and stomach qi deficiency, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, consumptive cough with hemoptysis, insufficient essence and blood, and internal heat and thirst. Modern research has also discovered that Polygonatum contains abundant polysaccharides, steroidal saponins, flavonoids, and other bioactive components, possessing antioxidant, anti-aging, blood sugar-lowering, and immune-enhancing effects. Its application in the pharmaceutical, health product, and food industries is becoming increasingly widespread.

[0003] With increasing emphasis on health and wellness, market demand for Polygonatum sibiricum continues to rise. Wild Polygonatum resources are becoming increasingly depleted due to over-harvesting, making artificial cultivation the primary means of meeting market demand. However, while the scale of artificial cultivation of Polygonatum sibiricum is expanding, pest and disease problems are becoming increasingly severe, especially the infestation of pathogens, which poses a significant threat to the yield and quality of Polygonatum sibiricum.

[0004] Currently, the detection and isolation of pathogens of Polygonatum sibiricum usually requires multiple steps and the use of various devices, which is cumbersome and inefficient, and not conducive to the rapid detection of pathogens of Polygonatum sibiricum. Therefore, a rapid detection and isolation device for pathogens of Polygonatum sibiricum is proposed to address the above problems. Utility Model Content

[0005] In order to overcome the shortcomings of the existing technology and solve at least one of the technical problems mentioned in the background art, this utility model proposes a rapid detection and separation device for Polygonatum pathogens.

[0006] The technical solution adopted by this utility model to solve its technical problem is: a rapid detection and separation device for Polygonatum pathogens, including a detection box; the detection box includes a pretreatment chamber and a detection chamber, the pretreatment chamber is hinged to an upper chamber cover, the pretreatment chamber is provided with an ultrasonic treatment mechanism and a grinding and filtering mechanism, the ultrasonic treatment mechanism includes a cleaning tank, the bottom inner side of the cleaning tank is provided with an ultrasonic generator, the detection chamber is hinged to a chamber door, the inner edge of the chamber door is provided with a silicone sealing strip, and the detection chamber is provided with a Polygonatum pathogen detection mechanism.

[0007] Preferably, a water level scale is fixedly installed on the inner wall of the cleaning tank, and a drain pipe is fixedly installed on the lower part of the side wall of the cleaning tank. The outer end of the drain pipe passes through the pretreatment chamber and is equipped with a valve body.

[0008] Preferably, the grinding and filtering mechanism includes a magnetically attached base that can be detachably installed in the pretreatment chamber, a tank rack that is magnetically attached to the magnetically attached base, a ceramic grinding tank that is mounted on the tank rack, a tank cover that is detachably installed on the top of the ceramic grinding tank, a micro motor that is mounted on the tank cover, a grinding head that is detachably installed through the tank cover at the output end of the micro motor, a lower grinding disc that is fixedly installed on the bottom inner side of the ceramic grinding tank, and a bacterial liquid temporary storage tube that is detachably installed at the bottom of the ceramic grinding tank.

[0009] Preferably, the lower grinding disc has a grinding area that is adapted to the grinding head, and the lower grinding disc has a set of annular filter frames that are located outside the grinding area and are arranged in a concentric circle structure with the grinding area.

[0010] Preferably, the Solomon's seal pathogen detection mechanism includes a drive motor installed in the detection chamber, a turntable installed at the output end of the drive motor, multiple detection zones evenly arranged on the turntable, a slot fixedly installed on each detection zone, a colloidal gold detection card detachably installed on the slot, and a sample application hole opened on the colloidal gold detection card.

[0011] Preferably, the detection box is provided with a side support, and a pathogen display scanner is provided on the side support, which is compatible with the colloidal gold detection card.

[0012] Preferably, the testing chamber is equipped with multiple UV sterilization lamp strips, and the UV sterilization lamp strips are located on the left and right sides of the testing chamber.

[0013] The advantages of this utility model are:

[0014] This invention integrates the cleaning, grinding and separation of bacterial solution, and pathogen detection of Polygonatum into a detection box, avoiding the use of multiple separate devices and enabling rapid detection of Polygonatum.

[0015] This invention uses an ultrasonic processing mechanism to remove bacteria and impurities from the surface of a sample of Polygonatum odoratum. A grinding and filtering mechanism is then used to grind the ultrasonically cleaned Polygonatum odoratum and collect the separated bacterial solution.

[0016] This invention utilizes the structural design of a pathogen detection mechanism for Polygonatum sibiricum to detect whether the separated bacterial solution contains pathogens. This mechanism can then be used for pathogen identification, drug sensitivity testing, and other purposes. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of this utility model;

[0019] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0020] Figure 3 This is a partial structural schematic diagram of the present invention;

[0021] Figure 4 This is a partial cross-sectional view of the grinding and filtering mechanism;

[0022] Figure 5 This is a partial cross-sectional view of the ultrasonic processing mechanism.

[0023] In the diagram: 1. Pretreatment chamber; 2. Detection chamber; 3. Upper chamber cover; 4. Cleaning tank; 5. Ultrasonic generator; 6. Chamber door; 7. Silicone sealing strip; 8. Water level scale mark; 9. Pipeline; 10. Valve body; 11. Magnetic base; 12. Tank rack; 13. Ceramic grinding jar; 14. Tank cover; 15. Micro motor; 16. Grinding head; 17. Lower grinding disc; 18. Bacterial liquid storage tube; 19. Grinding area; 20. Annular filter rack; 21. Drive motor; 22. Turntable; 23. Detection area; 24. Slot; 25. Colloidal gold detection card; 26. Sample dispensing port; 27. Side support; 28. Pathogen display scanner; 29. ​​UV sterilization lamp strip. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0025] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail.

[0026] This application discloses a rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum. (Refer to...) Figures 1-5A rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum includes a detection chamber. The detection chamber is made of stainless steel, and a power supply and an electrical control box are located on the side of the detection chamber. The power supply provides power to the electrical components in the device, and the electrical control box contains an STM32 microcontroller with a built-in preset program (which can automatically match parameters for Polygonatum sibiricum pathogenic bacteria). The electrical control box is electrically connected to the electrical components in the device. The detection chamber includes a pretreatment chamber 1 and a detection chamber 2. The pretreatment chamber 1 is hinged to an upper chamber cover 3, which facilitates the user to open the pretreatment chamber 1. The pretreatment chamber 1 is equipped with an ultrasonic treatment mechanism and a grinding and filtering mechanism, which integrates the pretreatment of the Polygonatum sibiricum to be detected. The Polygonatum sibiricum is ultrasonically cleaned to remove bacteria and impurities attached to the sample surface, and the ultrasonically cleaned Polygonatum sibiricum is then ground. The ultrasonic treatment mechanism includes a cleaning tank 4, an ultrasonic generator 5 installed on the inner bottom of the cleaning tank 4, and a door 6 hinged to the detection chamber 2. A silicone sealing strip 7 is installed on the inner edge of the door 6 to ensure the airtightness of the detection chamber 2 area and prevent other bacteria from entering the detection chamber 2. The detection chamber 2 is equipped with a Solomon's seal pathogen detection mechanism. Through the structure of the Solomon's seal pathogen detection mechanism, the bacterial solution is tested to see if it contains pathogens. This can then be used for pathogen identification, drug sensitivity testing, etc.

[0027] Reference Figure 3 and Figure 5 The inner wall of the cleaning tank 4 is fixedly equipped with a water level scale line 8, which makes it easy for staff to control the addition of an appropriate amount of sterile water to the cleaning tank 4. A drain pipe 9 is fixedly installed on the lower side wall of the cleaning tank 4. The outer end of the drain pipe 9 passes through the pretreatment chamber 1 and is equipped with a valve body 10 to discharge the wastewater in the cleaning tank 4.

[0028] Reference Figure 3 and Figure 4 The grinding and filtering mechanism includes a magnetic base 11 that can be detachably installed in the pretreatment chamber 1. A tank rack 12 is magnetically attached to the magnetic base 11. A ceramic grinding tank 13 is installed on the tank rack 12. A tank cover 14 is detachably installed on the top of the ceramic grinding tank 13. A micro motor 15 is installed on the tank cover 14. A grinding head 16 is detachably installed through the output end of the micro motor 15 through the tank cover 14. A lower grinding disc 17 is fixedly installed on the bottom inner side of the ceramic grinding tank 13. A bacterial liquid storage tube 18 is detachably installed at the bottom of the ceramic grinding tank 13. Through the structural arrangement of the grinding and filtering mechanism, the ultrasonically cleaned Polygonatum is ground and the bacterial liquid is collected for testing.

[0029] Reference Figure 4The lower grinding disc 17 is provided with a grinding area 19, which is adapted to the grinding head 16. A set of annular filter frames 20 is provided on the lower grinding disc 17. The annular filter frames 20 are located outside the grinding area 19 and are arranged in a concentric circle structure with the grinding area 19. Through this structure, impurities of the ground Polygonatum are filtered to obtain the bacterial liquid of ground Polygonatum.

[0030] Reference Figure 1 and Figure 2 The Solomon's seal pathogen detection mechanism includes a drive motor 21 installed in the detection chamber 2. A turntable 22 is installed on the output end of the drive motor 21. Multiple detection zones 23 are evenly arranged on the turntable 22. Slots 24 are fixedly installed on the detection zones 23. Colloidal gold detection cards 25 are detachably installed on the slots 24. The colloidal gold detection cards 25 have sample application holes 26. Through this structure, multiple test samples can be processed, reducing the error of sample detection and facilitating operation by staff.

[0031] Reference Figure 1 and Figure 2 The testing chamber is equipped with a side support 27, on which a pathogen display scanner 28 is mounted. The pathogen display scanner 28 is compatible with the colloidal gold test card 25. The pathogen display scanner 28 automatically identifies the T line and C line of the bacterial solution on the colloidal gold test card 25. When the pathogen display scanner 28 tests the colloidal gold test card 25, if the C line is colored but the T line is not colored, it is "negative" (no pathogens); if both the C line and the T line are colored, it is "positive" (contains pathogens). At the same time, the pathogen concentration corresponding to the gray value of the T line is displayed.

[0032] Reference Figure 1 The testing chamber 2 is equipped with multiple UV sterilization lamp strips 29, which are located on the left and right sides of the testing chamber 2. With this structure, after the test is completed, the UV sterilization lamp strips 29 will irradiate and sterilize the testing chamber 2, which will facilitate the reuse of the devices in the testing chamber 2 in the future.

[0033] Working principle: When performing pathogen detection and isolation on a sample of Polygonatum sibiricum;

[0034] Staff members use the ultrasonic processing mechanism and grinding and filtering mechanism set in the pretreatment chamber 1 to pre-treat the Polygonatum sibiricum to be tested, remove bacteria and impurities attached to the sample surface, and grind the Polygonatum sibiricum after ultrasonic cleaning and collect the separated bacterial solution for testing.

[0035] By using the structure of the ultrasonic processing mechanism, bacteria and impurities attached to the surface of the tested Polygonatum sample are removed. By adding an appropriate amount of sterile water to the cleaning tank 4, placing the Polygonatum in the cleaning tank 4, controlling the operation of the ultrasonic generator 5, cleaning for 5-6 minutes, and changing the sterile water in the cleaning tank 4 several times.

[0036] Through the structure of the grinding and filtering mechanism, the ultrasonically cleaned Polygonatum is ground and the separated bacterial solution is collected and tested. The Polygonatum sample slice is placed in the lower grinding plate 17 of the ceramic grinding jar 13, and an appropriate amount of sterile physiological saline (diluting the bacterial solution) is added to the lower grinding plate 17. The jar lid 14 is closed on the ceramic grinding jar 13, and the operation of the micro motor 15 is controlled to grind the Polygonatum sample. The ground sample bacterial solution is filtered through the annular filter rack 20 to remove impurities from the sample solution, and the ground Polygonatum bacterial solution is collected and flows into the bacterial solution temporary storage tube 18.

[0037] By utilizing the structural design of the Polygonatum sibiricum pathogen detection mechanism, the bacterial solution in the bacterial solution storage tube 18 is dripped through the sample application hole 26 on the colloidal gold detection card 25. Multiple colloidal gold detection cards 25 are then placed on the detection area 23 of the turntable 22, with the cards installed in slots 24. The turntable 22 rotates by controlling the drive motor 21. The detection area 23 on the turntable 22 passes sequentially through the pathogen display scanner 28, automatically identifying the T and C lines of the bacterial solution on the colloidal gold detection cards 25. When the pathogen display scanner 28 detects the colloidal gold detection cards 25, if the C line is colored but the T line is not, it is "negative" (no pathogen); if both the C and T lines are colored, it is "positive" (contains pathogen). Simultaneously, the pathogen concentration corresponding to the grayscale value of the T line is displayed (semi-quantitative result).

[0038] The detection chamber 2 is equipped with multiple UV sterilization lamp strips 29, which are located on the left and right sides of the detection chamber 2. With this structure, after the detection is completed, the UV sterilization lamp strips 29 will irradiate and sterilize the detection chamber 2, which will facilitate the reuse of the devices in the detection chamber 2 in the future.

[0039] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum, comprising a detection chamber; characterized in that: The testing chamber includes a pretreatment chamber (1) and a testing chamber (2). The pretreatment chamber (1) is hinged to an upper chamber cover (3). The pretreatment chamber (1) is equipped with an ultrasonic treatment mechanism and a grinding and filtering mechanism. The ultrasonic treatment mechanism includes a cleaning tank (4). An ultrasonic generator (5) is installed on the inner bottom of the cleaning tank (4). The testing chamber (2) is hinged to a chamber door (6). A silicone sealing strip (7) is installed on the inner edge of the chamber door (6). The testing chamber (2) is equipped with a Solomon's seal pathogen detection mechanism.

2. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 1, characterized in that: Water level markings (8) are fixedly installed on the inner wall of the cleaning tank (4), and a drain pipe (9) is fixedly installed on the lower part of the side wall of the cleaning tank (4). The outer end of the drain pipe (9) passes through the pretreatment chamber (1) and is equipped with a valve body (10).

3. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 1, characterized in that: The grinding and filtering mechanism includes a magnetic suction seat (11) that can be detachably installed in the pretreatment chamber (1). A tank rack (12) is magnetically attached to the magnetic suction seat (11). A ceramic grinding tank (13) is installed on the tank rack (12). A tank cover (14) is detachably installed on the top of the ceramic grinding tank (13). A micro motor (15) is installed on the tank cover (14). A grinding head (16) is detachably installed through the tank cover (14) at the output end of the micro motor (15). A lower grinding disc (17) is fixedly installed on the bottom inner side of the ceramic grinding tank (13). A bacterial liquid temporary storage tube (18) is detachably installed at the bottom of the ceramic grinding tank (13).

4. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 3, characterized in that: The lower grinding disc (17) is provided with a grinding area (19), which is adapted to the grinding head (16). A set of annular filter frames (20) is provided on the lower grinding disc (17). The annular filter frames (20) are located outside the grinding area (19) and are arranged in a concentric circle structure with the grinding area (19).

5. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 1, characterized in that: The Solomon's seal pathogen detection mechanism includes a drive motor (21) installed in the detection chamber (2). A turntable (22) is installed on the output end of the drive motor (21). Multiple detection zones (23) are evenly arranged on the turntable (22). A slot (24) is fixedly installed on the detection zone (23). A colloidal gold detection card (25) is detachably installed on the slot (24). A sample application hole (26) is opened on the colloidal gold detection card (25).

6. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 5, characterized in that: The detection box is provided with a side support (27), and a pathogen display scanner (28) is provided on the side support (27). The pathogen display scanner (28) is compatible with the colloidal gold detection card (25).

7. The rapid detection and isolation device for pathogenic bacteria of Polygonatum sibiricum according to claim 1, characterized in that: The detection chamber (2) is equipped with multiple UV sterilization lamp strips (29), and the UV sterilization lamp strips (29) are located on the left and right sides of the detection chamber (2).

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