Variable path length uv detection device and detection system thereof

Abstract

The utility model provides a variable optical path ultraviolet detection device and detection system thereof, including drive arrangement, the output of drive arrangement is connected the sliding block, is provided with the connecting piece on the sliding block, the connecting piece connects the optical fiber of probe, the one end of probe optical fiber is placed in the flow cell movably, and the flow cell is provided with liquid inlet end and liquid outlet end respectively, and the below of flow cell is provided with collimating plano -convex lens and UV LED lamp respectively, the liquid inlet end of the flow cell of variable optical path detector is connected the solution to be measured sampling port through the pipeline, and the liquid outlet end of flow cell is connected pump and waste outlet through the valve respectively, the valve includes two positions, when being in first position, the liquid outlet end of flow cell is communicated with pump, when being in second position, the pump is communicated with waste outlet, the utility model has the advantages that the optical path can be automatically adjusted, guarantees the symmetry and integrality of peak type, and the actual sample concentration is obtained.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of optical detection, in particular to a variable optical path ultraviolet detection device and a detection system thereof. BACKGROUND

[0002] Biotechnological processes are used to produce a wide variety of products, such as proteins, cells, tissues, carbohydrates and vaccines. The monitoring of the manufacturing process can be done by in situ analysis, offline monitoring or online monitoring. Biopharmaceutical processes are time consuming and are usually performed in batch processing mode. Throughout the production process, continuous processing for each chromatography step, this part requires sampling at each stage, and then analysis, to ensure the efficiency of the process. Analysis of these samples will provide information that will enable the process parameters to be adjusted between each step of the manufacturing process. During the process development, samples need to be collected across multiple points at each stage of the process and analyzed for UV absorbance. For a single molecule, this step needs to be repeated multiple times at each stage of the development process. In the separation and purification process of proteins in the biopharmaceutical field, the detection of protein concentration is required. Currently, data is collected and read by ultraviolet sensors for ultraviolet value detection. The currently used is fixed path measurement, which has the disadvantage that when the concentration is too high during measurement, an absorption peak flat head will occur, as shown in Figure 1 The occurrence of a flat head peak generally depends on the dynamic range of the sensor for absorption measurement, and in general, the normal flat head peak range is 2.5 Au. The detection of protein concentration can be achieved by offline sampling, but this detection method increases the process time and cannot accurately determine the protein concentration, and also causes the risk of product contamination. SUMMARY

[0003] In order to overcome the above-mentioned deficiencies of the prior art, the present application provides a variable optical path ultraviolet detection device and a detection system thereof. In the case of high concentration, the optical path is automatically adjusted, and the measurement of high concentration samples can be achieved by multiple optical path adjustments, so that the flat head peak does not occur, ensuring the symmetry and integrity of the peak shape, and obtaining the actual sample concentration.

[0004] To solve the above technical problems, the first aspect of the present application provides a variable optical path ultraviolet detection device, comprising a driving device, the output end of the driving device is connected with a sliding block, a connecting piece is arranged on the sliding block, the connecting piece is connected with a probe optical fiber, one end of the probe optical fiber is movably placed in a flow cell, the flow cell is respectively provided with an inlet end and an outlet end, a collimating plano-convex lens and a UV-LED lamp are respectively arranged below the flow cell.

[0005] In some embodiments, the driving device is a voice coil motor.

[0006] In some embodiments, the driving device is fixed by a mounting frame, the mounting frame comprises an upper top plate, the lower side of the upper top plate fixes the driving device, an L-shaped support plate is connected below the upper top plate, and a fixing plate is arranged on one side of the L-shaped support plate.

[0007] In some embodiments, a raised vertical bar is arranged on the L-shaped support plate, a groove is arranged on the sliding block corresponding to the raised vertical bar, the raised vertical bar is matched with the groove, and the sliding block can move in an up-and-down limited manner.

[0008] In some embodiments, the flow cell is fixed by a placement frame, the placement frame is in a shape of "匚", and a hole for the probe-carrying optical fiber to pass through is arranged at the top of the placement frame; the placement frame is connected with the fixing plate through a fixed cross beam; a collimating plano-convex lens is arranged in the bottom of the placement frame, and a UV-LED lamp is fixed under the bottom of the placement frame.

[0009] In some embodiments, a position sensor is arranged on one side of the L-shaped support plate, and the position sensor is fixed by a mounting plate.

[0010] In some embodiments, the flow cell comprises an outer shell, a hollow flow channel is arranged in the outer shell, an open upper end cover is arranged at the upper end of the outer shell, and a lower end cover with a plug is arranged at the bottom of the outer shell; one end of the probe optical fiber can move in the hollow flow channel; a liquid inlet end is arranged on one side of the bottom of the hollow flow channel, the liquid inlet end passes through the outer shell to communicate with the outside, a liquid outlet end is arranged on one side of the top of the hollow flow channel, and the liquid outlet end passes through the outer shell to communicate with the outside.

[0011] In some embodiments, a fixing and limiting device is arranged between the hollow flow channel and the opening of the upper end cover, the fixing and limiting device is a hollow end盖 sleeved on the upper end of the hollow flow channel, and one end of the probe optical fiber passes through the upper end cover and the hollow end cover to enter the hollow flow channel.

[0012] To address the above technical problems, the second aspect of the present disclosure provides a variable optical path ultraviolet detection system, comprising the above variable optical path detection device, the liquid inlet end of the flow cell of the variable optical path detector is connected to a sample inlet of a solution to be detected through a pipeline, and the liquid outlet end of the flow cell is respectively connected to a pump and a waste discharge port through a valve; the valve has two positions, when in the first position, the liquid outlet end of the flow cell is communicated with the pump, and when in the second position, the liquid outlet end of the flow cell is communicated with the waste discharge port.

[0013] In some embodiments, the valve is a two-position three-way valve.

[0014] In some embodiments, the pump is a syringe pump.

[0015] In some embodiments, the variable optical path detector is connected to the detection module via a probe fiber optic cable. The control and signal terminals of the detection module, the drive device, the UV-LED lamp, and the pump are respectively connected to a PLC controller, which is controlled by a host computer software system.

[0016] The beneficial effects of this invention are: 1. This invention uses a driving device to enable the optical fiber with probe to move, with a stroke of 0.01-5mm. If the initial optical path is 3mm, and the concentration is too high, such as absorbing 2.5 Au, the optical path will automatically be adjusted to 1 / 2 (1.5mm). According to the Lambert-Beer law, the measured value of this optical path is 1 / 2 of the original value. In this way, high-concentration samples can be measured through multiple optical path adjustments without the appearance of flat peaks, thus accurately measuring the actual concentration of the high-concentration sample.

[0017] 2. The variable optical path ultraviolet detection system uses a syringe pump to quantitatively collect the sample to be tested, and can detect the sample concentration in real time without contaminating the product. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of existing technology for measuring UV absorption at a fixed optical path.

[0019] Figure 2 This is a perspective view of the variable optical path ultraviolet detection device of the present invention.

[0020] Figure 3 This is a cross-sectional view of the variable optical path ultraviolet detection device of the present invention.

[0021] Figure 4 This is a side view of the variable optical path ultraviolet detection device of the present invention.

[0022] Figure 5 The detection system of this invention.

[0023] Figure 6 This is a measurement diagram of the variable optical path UV absorption of the present invention. Detailed Implementation

[0024] The invention will now be further described with reference to the accompanying drawings.

[0025] The technical content of the present invention is illustrated below through specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the present invention.

[0026] Before detailing the specific embodiments of this disclosure, some terms used in this disclosure will be explained first.

[0027] Unless otherwise defined below, all technical and scientific terms used herein are intended to have the same meaning as commonly understood by one of ordinary skill in the art. References to technical terms herein refer to techniques commonly understood in the art, including variations or equivalent substitutions of techniques that are obvious to one of ordinary skill in the art. While it is believed that the following terms will be well understood by one of ordinary skill in the art, the following definitions are set forth to better explain the invention. When a superior product name appears herein, it is intended to refer to the corresponding superior product. All patents, published patent applications, and publications cited herein are incorporated herein by reference.

[0028] The terms “connection,” “link,” “coupled,” or “coupled” used in this article are not limited to direct connections; they also include indirect connections.

[0029] As used herein, "online monitoring" or "real-time monitoring" refers to the real-time detection of certain parameters or properties of the buffer solution, reaction fluid, or fluid exiting the flow reactor during the use of the chromatography system, such as pH, pressure, flow rate, and conductivity. Unlike offline detection or analysis, online or real-time monitoring provides immediate feedback on the detection results.

[0030] The positional terms "up," "down," "left," "right," "front," and "back" used in this article are determined based on the layout direction of the accompanying drawings in the specification. They are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0031] Reference Figure 2 , Figure 3As shown, the variable optical path ultraviolet detection device comprises a voice coil motor 1, an output end of the voice coil motor 1 is connected with a sliding block 2, a connecting piece 3 is fixedly connected to the sliding block 2, and the connecting piece 3 is connected with a probe-carrying optical fiber 4; the voice coil motor 1 is fixed by a mounting frame, the mounting frame comprises an upper top plate 5, the lower side of the upper top plate 5 fixes the voice coil motor 1, and the upper top plate 5 is fixedly connected with an L-shaped support plate 6. A fixing plate 7 is arranged on one side of the L-shaped support plate 6. A [ shaped placement frame 8 is arranged below the connecting piece 3, the placement frame 8 is connected with the fixing plate 7 through a fixed cross beam 16, a hole for the probe-carrying optical fiber 4 to pass through is arranged at the top of the placement frame 8, a flow cell 9 is fixed on the bottom of the placement frame 8, the flow cell 9 comprises an outer shell 91, a hollow flow channel 92 is arranged in the outer shell 91, an open upper end cover 93 is arranged at the upper end of the outer shell 91, and a lower end cover 94 with a plug is arranged at the bottom of the outer shell 91. The probe-carrying optical fiber 4 passes through the open upper end cover 93 and enters the hollow flow channel 92, and can move up and down along the hollow flow channel 92. A fixing and limiting device 95 is sleeved and fixed at the upper end of the hollow flow channel 92, the fixing and limiting device 95 is located between the hollow flow channel 92 and the opening of the upper end cover 93 and is of a hollow structure, the diameter of the hollow structure is slightly larger than the diameter of the probe-carrying optical fiber 4, which facilitates the stability of the probe-carrying optical fiber 4 during up-and-down movement. A liquid inlet end 96 is arranged on one side of the bottom of the hollow flow channel 92, the liquid inlet end 96 passes through the outer shell 91 and communicates with the outside, a liquid outlet end 97 is arranged on one side of the top of the hollow flow channel 92, and the liquid outlet end 97 passes through the outer shell 91 and communicates with the outside. A collimating plano-convex lens 10 is arranged in the bottom of the placement frame 8 below the hollow flow channel 92, and a UV-LED lamp 11 is fixed below the bottom of the placement frame. The hollow flow channel 92, the collimating plano-convex lens 10 and the UV-LED lamp 11 are on the same vertical line. The light source UV-LED lamp 11 is standard-equipped with 258nm wavelength or 254nm wavelength. In order to monitor the stroke driven by the voice coil motor 1 in real time, a position sensor 12 is arranged on one side of the support plate 6, and the position sensor 12 is fixed by a mounting plate 13.

[0032] In order to enable the sliding block to move up and down stably, refer to Figure 4 a protruding vertical bar 14 is arranged on the L-shaped support plate 6, a groove 15 is arranged on the sliding block 2 corresponding to the protruding vertical bar 14, the protruding vertical bar 14 is matched with the groove 15, and the sliding block can move with up-and-down limiting.

[0033] refer to Figure 5As shown, the variable optical path ultraviolet detection system includes a variable optical path ultraviolet detector 20. The inlet 96 of the flow cell 9 of the variable optical path ultraviolet detector 20 is connected to the sampling port 21 of the solution to be tested via a pipe. The outlet 97 is connected to end a of a two-position three-way valve 22 via a pipe. End b of the two-position three-way valve 22 is connected to a syringe pump 23. End c of the two-position three-way valve 22 is connected to the waste outlet 24 via a pipe. The optical fiber 4 with probe of the variable optical path ultraviolet detector 20 is connected to the detection module 25. The control and signal terminals of the detection module 25, voice coil motor 1, UV-LED lamp 11, and syringe pump 23 are respectively connected to a PLC controller 26. The PLC controller 26 is controlled by a host computer software system 27.

[0034] In use, the two-position three-way valve 22 is in the first position, that is, the a end of the two-position three-way valve 22 is connected to the b end of the two-position three-way valve 22. At this time, the syringe pump 23 is connected to the flow cell 9. Under the action of the syringe pump 23, the sample to be tested enters the flow cell 9 from the sample port 21 of the solution to be tested. The height of the flow cell 9 is 0-15mm. At this time, the voice coil motor 1 drives the optical fiber 4 with probe to move up and down in the flow cell with a minimum step distance of 0.005mm. If the concentration is too high, the optical path will be automatically adjusted to 1 / 2 of the original value. According to the Lambert-Beer law, the measured value of the optical path is 1 / 2 of the original value. In this way, the measurement of high concentration samples can be achieved by adjusting the optical path multiple times without the appearance of a flat peak. In this way, the actual concentration of the high concentration sample can be accurately measured. Then, the syringe pump 23 is driven to extract the sample from the flow cell 9. The two-position three-way valve 22 is then in its second position, i.e., its b-end is connected to its c-end. At this time, the syringe pump 23 is connected to the waste discharge port 24, recovering the sample to be tested. Following this method, high-concentration samples can be measured through multiple optical path adjustments without the appearance of a flat-top peak. (Refer to...) Figure 6 As shown, this allows for the accurate measurement of the actual concentration of the high-concentration sample. Conversely, as the absorption peak decreases, the optical path length is increased according to the change in absorbance to ensure the symmetry and integrity of the peak shape.

Claims

1. A variable optical path ultraviolet detection device, characterized in that, Comprising a driving device, an output end of the driving device is connected to a sliding block, a connecting piece is arranged on the sliding block, the connecting piece is connected to an optical fiber with a probe, one end of the probe optical fiber is movably arranged in a flow cell, the flow cell is respectively provided with a liquid inlet end and a liquid outlet end, and a collimating plano-convex lens and a UV-LED lamp are respectively arranged below the flow cell.

2. The variable optical path ultraviolet detection device according to claim 1, characterized in that, The driving device is a voice coil motor.

3. The variable optical path ultraviolet detection device according to claim 1, characterized in that, The driving device is fixed by a mounting bracket, the mounting bracket comprises an upper top plate, a lower side of the upper top plate fixes the driving device, a lower part of the upper top plate is connected to an L-shaped support plate, and a fixing plate is arranged on one side of the L-shaped support plate.

4. The variable optical path ultraviolet detection device according to claim 3, characterized in that, A raised vertical strip is arranged on the L-shaped support plate, a groove is arranged on the sliding block corresponding to the raised vertical strip, the raised vertical strip is matched with the groove, and the sliding block can move in a vertically limited manner.

5. The variable optical path ultraviolet detection device according to claim 3, characterized in that, The flow cell is fixed by a placement frame, the placement frame is in a shape of "匚", a top of the placement frame is provided with a hole for the probe-carrying optical fiber to pass through; the placement frame is connected to the fixing plate via a fixed cross beam; a collimating plano-convex lens is arranged in a bottom of the placement frame, and a UV-LED lamp is fixed below the bottom of the placement frame.

6. The variable optical path ultraviolet detection device according to claim 3, characterized in that, A position sensor is arranged on one side of the L-shaped support plate, and the position sensor is fixed by a mounting plate.

7. The variable optical path ultraviolet detection device according to claim 1, characterized in that, The flow cell comprises an outer shell, a hollow flow channel is arranged in the outer shell, an upper end of the outer shell is provided with an upper end cover with an opening, a bottom of the outer shell is provided with a lower end cover with a plug; one end of the probe optical fiber can move in the hollow flow channel; a liquid inlet end is arranged on one side of a bottom of the hollow flow channel, the liquid inlet end passes through the outer shell to communicate with the outside, a liquid outlet end is arranged on one side of a top of the hollow flow channel, and the liquid outlet end passes through the outer shell to communicate with the outside.

8. The variable optical path ultraviolet detection device according to claim 7, characterized in that, A fixing and limiting device is arranged between the hollow flow channel and the opening of the upper end cover, the fixing and limiting device is a hollow end cover sleeved on an upper end of the hollow flow channel, and one end of the probe optical fiber passes through the upper end cover and the hollow end cover to enter the hollow flow channel.

9. A variable optical path ultraviolet detection system, characterized in that, Comprising the variable optical path detection device according to any one of claims 1-8, a liquid inlet end of a flow cell of the variable optical path detector is connected to a sample solution sampling port via a pipeline, a liquid outlet end of the flow cell is respectively connected to a pump and a waste discharge port via a valve; the valve has two positions, when in a first position, the liquid outlet end of the flow cell is communicated with the pump, and when in a second position, the liquid outlet end of the flow cell is communicated with the waste discharge port.

10. The variable optical path ultraviolet detection system according to claim 9, characterized in that, The valve is a two-position three-way valve.

11. The variable optical path ultraviolet detection system according to claim 9, characterized in that, The pump is a syringe pump.

12. The variable optical path ultraviolet detection system according to claim 9, characterized in that, The probe-carrying optical fiber of the variable optical path detector is connected to a detection module, control and signal terminals of the detection module, the driving device, the UV-LED lamp and the pump are respectively connected to a PLC controller, and the PLC controller is controlled by an upper computer software system.

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