An immunochromatographic test strip

Abstract

The application discloses an immunochromatography test paper, and relates to the field of biological rapid detection.The immunochromatography test paper comprises a bottom shell, a first test strip, a second test strip and a top plate assembly.The test paper is divided into the first test strip and the second test strip, and the overlapping section of the conjugate pad is placed above the elastic pad and the solid-phase membrane of the second test strip.Because the initial thickness of the elastic pad is greater than the thickness of the solid-phase membrane, the contact between the conjugate pad and the solid-phase membrane in a natural state is prevented.After the sample is added, the sample is fully reacted with the conjugate pad, and after the reaction is completed, the overlapping section above the elastic pad is pressed down until the overlapping section is in contact with the solid-phase membrane by pressing the sliding block on the overlapping section and the elastic pad, and the reaction solution in the conjugate pad continues to flow to the solid-phase membrane to complete the reaction with the T line and the C line.The segmented reaction of one-time sample adding is realized, the sensitivity is improved, and the subsequent reaction can be continued by pressing the pressing sliding block on the top plate.

Description

Technical Field

[0001] This application relates to the field of rapid biological detection, and in particular to an immunochromatographic test strip. Background Technology

[0002] Immunochromatographic test strips are a commonly used product in clinical practice. Based on the testing purpose, they can be divided into quantitative chromatographic test strips and qualitative chromatographic test strips. Based on their structure, they can be divided into bare chromatographic test strips and combined chromatographic test strips (test strip + outer shell). Combined chromatographic test strips have a sample application well and an observation well, and the outer shell structure protects the internal chromatographic test strip, thus making them more widely used.

[0003] Chinese patent CN103364547A discloses a multi-parameter immunochromatographic test strip and its preparation method. However, the immunochromatographic test strip of this patent has low sensitivity because the reaction time between the analyte in the sample and the marker in the conjugate membrane is too short.

[0004] In practical field applications, the marker in the conjugate pad is usually placed separately in an external reactor. The sample is first added to the external reactor, and after reacting for a period of time, the reaction solution is then added dropwise to the sample well of the test strip. However, this method requires an additional reactor, which increases the number of steps and limits its widespread application. Utility Model Content

[0005] This application provides an immunochromatographic test strip that can achieve a full reaction between the sample and the conjugate pad without the need for an external reactor, and is simple to operate.

[0006] This application provides an immunochromatographic test strip, including a bottom shell, a first test strip, a second test strip, and a top plate assembly. The first test strip is disposed within the bottom shell and includes a first base plate and a sample pad and a conjugate pad sequentially disposed on the first base plate along its length. In the length direction, the conjugate pad has an overlapping section extending to the outside of the first base plate. The second test strip is disposed within the bottom shell and includes a second base plate and an elastic pad, a solid phase membrane, and an absorbent pad sequentially disposed on the second base plate along its length. The initial thickness of the elastic pad is greater than the thickness of the solid phase membrane. The overlapping section is stacked on top of the elastic pad and the solid phase membrane. The top plate assembly includes a top plate and a pressing slider. The top plate is connected to the bottom shell and has a groove whose position corresponds to the elastic pad in the thickness direction. The pressing slider is disposed within the groove and is used to press the overlapping section and the elastic pad so that the overlapping section contacts the solid phase membrane.

[0007] Preferably, the bottom shell is provided with a mounting groove, and the first test strip and the second test strip are disposed in the mounting groove.

[0008] Preferably, the bottom shell and the top plate are detachably connected.

[0009] Preferably, both the sample pad and the conjugate pad are disposed on the upper surface of the first base plate, and the sample pad and the conjugate pad are connected; the overlapping section of the conjugate pad is stacked on top of the elastic pad and the solid phase membrane.

[0010] Preferably, the elastic pad of the second test strip is configured as a sponge pad or a rubber pad.

[0011] Preferably, the initial thickness of the elastic pad is 1.2 to 2 times the thickness of the solid phase film.

[0012] Preferably, the top plate is provided with a first observation hole and a second observation hole, which are used to observe the T-line and C-line of the solid phase film, respectively; the lower surface of the top plate is provided with a plurality of contact limiting parts, which are spaced apart along the length direction, and the contact limiting parts are used to press the first test strip or the second test strip into the bottom shell.

[0013] Preferably, the groove extends along the length direction, and the pressing slider is slidably disposed within the groove along the length direction.

[0014] Preferably, the top plate is provided with an anti-detachment groove; the pressing slider is provided with a mating part, which is located in the anti-detachment groove. The mating part can slide relative to the anti-detachment groove in the length direction, and the thickness of the mating part is less than the inner width of the anti-detachment groove, so that the mating part can float relative to the anti-detachment groove in the thickness direction.

[0015] Preferably, the pressing slider is provided with a positioning part, and the top plate is provided with a positioning groove; when the pressing slider slides above the elastic pad, the positioning part is inserted into the positioning groove along the length direction.

[0016] The immunochromatographic test strip of this application has at least the following beneficial effects:

[0017] The immunochromatographic test strip of this application consists of a first strip and a second strip. During testing, the second strip is pre-installed in the bottom shell, and then the first strip is placed inside the bottom shell. Before placement, the overlapping section of the conjugate pad is positioned above the elastic pad and solid membrane of the second strip. Since the initial thickness of the elastic pad is greater than the thickness of the solid membrane, it prevents the conjugate pad from contacting the solid membrane under natural conditions. After the sample is added, the sample reacts fully with the conjugate pad first. After the reaction is complete, the overlapping section of the conjugate pad and the elastic pad are squeezed by pressing the slider, so that the thickness of the elastic pad is compressed. The overlapping section covering the elastic pad can then be pressed down until the overlapping section contacts the solid membrane. The reaction solution in the conjugate pad continues to flow to the solid membrane, completing the reaction with the T-line and C-line. This application achieves a segmented reaction with a single sample addition, improving sensitivity. The subsequent reaction can be continued by pressing the slider on the top plate. Attached Figure Description

[0018] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0019] Figure 1 This is an explosion diagram of the immunochromatographic test strip of this application;

[0020] Figure 2 This is a cross-sectional view of the immunochromatographic test strip of this application;

[0021] Figure 3 yes Figure 2 A partial schematic diagram;

[0022] Figure 4 This is an isometric view of the roof slab assembly;

[0023] Figure 5 yes Figure 4 Enlarged view of point A in the middle;

[0024] The annotations in the attached figures are explained as follows:

[0025] 100, bottom shell; 100a, mounting slot; 100b, screw connection hole;

[0026] 200. First test strip; 210. First base plate; 220. Sample pad; 230. Bonding pad; 231. Overlapping section;

[0027] 300, Second test strip; 310, Second base plate; 320, Elastic pad; 330, Solid phase membrane; 331, T-line section; 332, C-line section; 340, Absorbent pad;

[0028] 400, Top plate assembly; 410, Top plate; 410a, Slide groove; 410b, First observation hole; 410c, Second observation hole; 410d, Sample feeding hole; 410e, Anti-detachment groove; 410f, Positioning groove; 411, Abutting and limiting part; 420, Pressing slider; 421, Mating part; 422, Positioning part; 420a, Arc-shaped surface; 420b, Sliding plane. Detailed Implementation

[0029] The features and exemplary embodiments of various aspects of this application will be described in detail below. To make the objectives, technical solutions, and advantages of this application clearer, the application will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only intended to explain this application and not to limit it. For those skilled in the art, this application can be implemented without some of these specific details. The following description of the embodiments is merely to provide a better understanding of this application by illustrating examples.

[0030] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising..." does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0031] like Figure 1 and Figure 2 As shown, this embodiment discloses an immunochromatographic test strip that maintains the convenience of single sample addition while increasing the sensitivity of the reaction.

[0032] like Figure 1 and Figure 2 As shown, the immunochromatographic test strip includes a bottom shell 100, a first test strip 200, a second test strip 300, and a top plate assembly 400. The first test strip 200 and the second test strip 300 are both disposed inside the bottom shell 100, and the top plate assembly 400 is disposed on the upper surface of the bottom shell 100 to seal the bottom shell 100.

[0033] like Figure 2 As shown, in order to facilitate understanding of the technical solution of this embodiment, the following direction definitions are made first: the left and right directions are defined as the length direction, and the height direction is defined as the thickness direction.

[0034] like Figure 1As shown, the bottom shell 100 is rectangular in shape and has a downwardly recessed mounting groove 100a. The mounting groove 100a has a rectangular cross-sectional shape. The first test strip 200 and the second test strip 300 are both disposed within the mounting groove 100a. The top plate assembly 400 is connected to the upper end face of the mounting groove 100a, thereby closing the mounting groove 100a. In this embodiment, the inner peripheral wall of the mounting groove 100a can limit the first test strip 200 and the second test strip 300, ensuring that the two test strips (i.e., the first test strip 200 and the second test strip 300) are stably positioned within the mounting groove 100a. The bottom shell 100 and the top plate assembly 400 work together to protect the first test strip 200 and the second test strip 300.

[0035] like Figure 1 As shown, the upper groove end face of the mounting groove 100a is detachably connected to the top plate 410 of the top plate assembly 400. The detachable connection methods include:

[0036] The first method (the preferred method in this embodiment): such as Figure 1 As shown, screw connection holes 100b are provided at the four corners of the upper slot end face, and screw holes are provided on the top plate 410. Screws (not shown) pass through the screw holes one by one and are threaded into the screw connection holes 100b, thereby fitting and fastening the lower surface of the top plate 410 to the upper slot end face.

[0037] The second method: A first adhesive layer is provided on the lower surface of the top plate 410, and a second adhesive layer corresponding to the first adhesive layer is provided on the upper groove end face. The first adhesive layer and the second adhesive layer can be bonded in the thickness direction.

[0038] In this embodiment, the bottom shell 100 and the top plate 410 are connected in a detachable manner, which facilitates the installation and sealing of the test strips, and also facilitates the reuse of the bottom shell 100 and the top plate 410.

[0039] like Figure 2 and Figure 3As shown, the first test strip 200 includes a first base plate 210, a sample pad 220, and a bonding pad 230. The first base plate 210 is rectangular flat and made of PVC. The sample pad 220 is attached to the first base plate 210, with one end of the sample pad 220 overlapping the upper surface of the first end of the bonding pad 230, which is disposed on the upper surface of the first base plate 210. In the length direction, the second end of the bonding pad 230 extends to the outside of the first base plate 210. This part of the bonding pad 230 that extends to the outside of the first base plate 210 in the length direction is called the overlapping section 231 of the bonding pad 230. The length L1 of the overlapping section 231 can be 2-10 mm (mm: millimeter). When the first test strip 200 is installed in the mounting groove 100a of the bottom shell 100, the overlapping section 231 overlaps the elastic pad 320 of the second test strip 300, and the overlapping section 231 extends to the upper side of the solid phase membrane 330 of the second test strip 300 in the length direction.

[0040] like Figure 2 and Figure 3 As shown, in this embodiment, the sample pad 220 and the conjugate pad 230 are sequentially arranged on the first base plate 210 along the length direction, and the overlapping section 231 of the conjugate pad 230 covers the upper side of the elastic pad 320 and the solid phase membrane 330 of the second test strip 300. After the sample is added to the sample pad 220 and flows through the conjugate pad 230, the sample will react fully with the conjugate pad 230 first. After the reaction is completed, the overlapping section 231 and the elastic pad 320 are squeezed by the pressing slider 420 on the top plate assembly 400, so that the elastic pad 320 is deformed, and the overlapping section 231 of the conjugate pad 230 can contact the solid phase membrane 330. The reaction liquid of the conjugate pad 230 continues to advance and completes the reaction with the T line 331 and the C line 332.

[0041] like Figure 2 As shown, the second test strip 300 includes a second base plate 310, an elastic pad 320, a solid phase membrane 330, and a water-absorbing pad 340; the second base plate 310 is rectangular flat and made of PVC; the elastic pad 320, the solid phase membrane 330, and the water-absorbing pad 340 are arranged sequentially along the length direction on the upper surface of the second base plate 310.

[0042] like Figure 3As shown, when the elastic pad 320 is not compressed by the pressing slider 420, the initial thickness of the elastic pad 320 is configured as D1, and the thickness of the solid phase membrane 330 is D2, where D1 is greater than D2, for example, D1 is 1.2 to 2 times D2, and more preferably, D1 is 1.5 times D2. When the elastic pad 320 is compressed by the pressing slider 420, the deformed thickness of the elastic pad 320 is configured as D3 (not shown), where D3 is less than D2. In the length direction, the gap between the elastic pad 320 and the solid phase membrane 330 is L2, and L2 ranges from 3 to 8 mm. In some preferred embodiments, the elastic pad 320 is made of sponge or rubber, and its shape is rectangular.

[0043] like Figure 3 As shown, in this embodiment, the second test strip 300, due to the presence of the elastic pad 320, prevents the overlapping section 231 from contacting the solid phase membrane 330 in its natural state (i.e., before being squeezed by the pressing slider 420), providing sufficient time for the sample to fully react with the substances in the binding pad 230, thereby improving the detection sensitivity.

[0044] like Figure 2 As shown, the solid membrane 330 is in the shape of a rectangular plate. The solid membrane 330 has T-line portions 331 and C-line portions 332 spaced apart along its length. The T-line portion 331 is the detection portion, and the C-line portion 332 is the quality control portion. This is prior art and will not be described in detail here.

[0045] like Figure 2 As shown, the absorbent pad 340 is disposed at the end of the solid membrane 330 away from the elastic pad 320, and the absorbent pad 340 overlaps the upper surface of the solid membrane 330. The absorbent pad 340 is used to absorb excess liquid.

[0046] like Figure 4 As shown, the top plate assembly 400 includes a top plate 410 and a pressing slider 420. The top plate 410 is rectangular flat and its size is large enough to completely cover the mounting groove 100a and seal the interior of the mounting groove 100a. The pressing slider 420 is used to press the overlapping section 231 of the bonding pad 230 and the elastic pad 320 so that the overlapping section 231 can contact the solid phase membrane 330.

[0047] like Figure 4As shown, a groove 410a is provided on the top plate 410, and a pressing slider 420 is movably disposed in the groove 410a. The groove 410a corresponds to the overlapping section 231 of the elastic pad 320 and the bonding pad 230 in the thickness direction. When it is necessary to contact the overlapping section 231 of the bonding pad 230 with the solid membrane 330, it is only necessary to push the pressing slider 420 so that the height of the elastic pad 320 becomes shorter after being pressed, thereby allowing the overlapping section 231 to overlap the solid membrane 330, and the reaction liquid in the bonding pad 230 can flow onto the solid membrane 330.

[0048] In this preferred embodiment, the groove 410a has a predetermined aspect ratio, the length of the groove 410a is greater than its width, and the groove 410a corresponds in the thickness direction to the portion of the bonding pad 230 disposed on the first base plate 210 and the portion extending to the outside of the first base plate 210 (i.e., the overlapping section 231). The width of the pressing slider 420 is equal to the width of the groove 410a, so that the pressing slider 420 can only move relative to the top plate 410 in the length and thickness directions.

[0049] In this embodiment, after the sample has fully reacted with the substance in the conjugate pad 230, the pressing slider 420 can be manually pushed downward to press the conjugate pad 230. Then, the pressing slider 420 is pushed along the length direction until the pressing slider 420 squeezes the elastic pad 320 and causes the overlapping section 231 of the conjugate pad 230 to overlap the solid membrane 330. During the process of the pressing slider 420 sliding along the length direction, the pressing slider 420 can push and concentrate the reaction liquid in the conjugate pad 230 to the overlapping section 231 of the conjugate pad 230, and quickly flow from the overlapping section 231 to the solid membrane 330.

[0050] like Figure 3 As shown, the lower end of the pressing slider 420 is provided with an arc-shaped surface 420a and a sliding plane 420b. The sliding plane 420b is parallel to the length direction, and the arc-shaped surface 420a and the sliding plane 420b are smoothly connected. In the horizontal direction, the arc-shaped surface 420a is located on the side facing the elastic pad 320, and the arc-shaped surface 420a facilitates pushing the pressing slider 420 to slide on the connecting pad 230.

[0051] like Figure 5As shown, in this preferred embodiment, the top plate 410 is provided with an anti-detachment groove 410e, which extends along the length direction. A mating part 421 is provided on the side wall of the pressing slider 420, and the mating part 421 is disposed within the anti-detachment groove 410e. The mating part 421 can move relative to the anti-detachment groove 410e in both the length and thickness directions. Specifically, the mating part 421 is rectangular in shape, and its thickness is less than the inner width of the anti-detachment groove 410e, for example, 0.2 to 0.5 times the inner width of the anti-detachment groove 410e. This allows the mating part 421 to float up and down within the anti-detachment groove 410e and slide along the length direction within the anti-detachment groove 410e. More preferably, the top plate 410 is symmetrically provided with two anti-detachment grooves 410e on both sides in the width direction, and the mating part 421 corresponds to each anti-detachment groove 410e.

[0052] In this embodiment, the top plate 410 restricts the pressing slider 420 from detaching from the top plate 410 by the anti-detachment groove 410e, making the structure more compact and easier to use. Furthermore, by designing the specifications of the mating part 421 and the anti-detachment groove 410e, the pressing slider 420 can have degrees of freedom in both the length and thickness directions relative to the top plate 410, which facilitates the pressing slider 420 pressing down on the connecting pad 230 and pushing the pressing slider 420 along the length direction.

[0053] like Figure 3 As shown, in this preferred embodiment, a positioning part 422 is provided on one side of the pressing slider 420 along the length direction. The positioning part 422 is rectangular in shape, and the top plate 410 is provided with a positioning groove 410f. The positioning groove 410f corresponds vertically to the solid phase membrane 330 in the thickness direction. When the pressing slider 420 is pushed along the length direction to the top of the elastic pad 320 and squeezes the elastic pad 320, the positioning part 422 is just inserted into the positioning groove 410f along the length direction. Since the elastic pad 320 is compressed at this time and has an elastic force to return to its original position, the elastic pad 320 has a tendency to push the pressing slider 420 upward. Since the positioning part 422 is inserted into the positioning groove 410f at this time, the positioning part 422 is pressed tightly into the positioning groove 410f by the elastic pad 320. At this time, even if the hand is released, the position of the pressing slider 420 will not slide randomly. In this embodiment, by designing the positioning part 422 and cooperating with the restoring elastic force of the elastic pad 320, the original posture can be maintained after squeezing the overlapping section 231 of the elastic pad 320 and the bonding pad 230, so that the reaction liquid in the bonding pad 230 can flow fully to the solid phase membrane 330.

[0054] In this preferred embodiment, the top plate 410 is further provided with the following structure:

[0055] like Figure 4As shown, the top plate 410 is provided with a first observation hole 410b and a second observation hole 410c, which are spaced apart along the length direction. The first observation hole 410b is positioned corresponding to the T-line portion 331 in the thickness direction, and is used to observe the T-line portion 331 in the thickness direction. The second observation hole 410c is positioned corresponding to the C-line portion 332 in the thickness direction, and is used to observe the C-line portion 332 in the thickness direction. The first and second observation holes 410b and 410c facilitate external personnel to observe the testing process and results. The top plate 410 is also provided with a sample application hole 410d, which is connected to the sample pad 220 in the thickness direction, allowing external personnel to drip samples from the sample application hole 410d onto the sample pad 220.

[0056] like Figure 4 As shown, the lower surface of the top plate 410 is provided with multiple contact limiting parts 411. These contact limiting parts 411 are used to press the first test strip 200 or the second test strip 300 into the bottom shell 100. Specifically, at least one contact limiting part 411 is used to press the absorbent pad 340 downwards, at least one contact limiting part 411 is used to press the solid phase membrane 330 downwards, and at least one contact limiting part 411 is used to press the sample pad 220 downwards. In this embodiment, the multiple contact limiting parts 411 restrict the position of the first test strip 200 and the second test strip 300 within the mounting groove 100a, ensuring the stability of the structure.

[0057] The working principle of the immunochromatographic test strip in this embodiment is as follows:

[0058] Assembly work:

[0059] The second test strip 300 is placed in the mounting groove 100a of the bottom shell 100. Then, the overlapping section 231 of the bonding pad 230 of the first test strip 200 is aligned with the elastic pad 320 and the solid phase membrane 330 of the second test strip 300 in the thickness direction. Then, the first test strip 200 is placed downward in the mounting groove 100a. After the first test strip 200 is placed, the overlapping section 231 of the bonding pad 230 is stacked on the elastic pad 320 of the second test strip 300, and at this time the overlapping section 231 does not contact the solid phase membrane 330 of the second test strip 300.

[0060] Then slide the pressing slider 420 on the top plate 410 to one end away from the elastic pad 320, and then connect the top plate 410 to the bottom shell 100.

[0061] Detection principle: The sample flows from the sample pad 220 to the conjugate pad 230, reacts with the marker in the conjugate pad 230 to form a reaction solution. After the sample has fully reacted with the conjugate pad 230, the manual pushes the pressing slider 420 downward, so that the bottom of the pressing slider 420 squeezes the conjugate pad 230. Then, the pressing slider 420 is pushed along the length direction, thereby pushing the reaction solution in the conjugate pad 230 into the overlapping section 231 of the conjugate pad 230. When the pressing slider 420 slides above the elastic pad 320, the pressing slider 420 squeezes the elastic pad 320. After the elastic pad 320 is squeezed and deformed, the overlapping section 231 of the conjugate pad 230 comes into contact with the solid phase membrane 330, and the reaction solution in the overlapping section 231 flows into the solid phase membrane 330.

[0062] The reaction liquid passes sequentially along the length of the solid membrane 330 through the T-line 331 and C-line 332. Finally, the excess liquid is absorbed by the absorbent pad 340, and the test is completed. The staff can observe the test results through the first observation hole 410b and the second observation hole 410c on the top plate 410.

[0063] The above description is merely a specific implementation of this application. Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working processes of the systems, modules, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here. It should be understood that the protection scope of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the protection scope of this application.

Claims

1. An immunochromatographic test strip, characterized in that, include: Bottom shell (100); The first test strip (200) is disposed inside the bottom shell (100). The first test strip (200) includes a first bottom plate (210) and a sample pad (220) and a bonding pad (230) disposed sequentially on the first bottom plate (210) along the length direction. In the length direction, the bonding pad (230) has an overlapping section (231) extending to the outside of the first bottom plate (210). The second test strip (300) is disposed inside the bottom shell (100). The second test strip (300) includes a second bottom plate (310) and an elastic pad (320), a solid membrane (330) and an absorbent pad (340) arranged sequentially on the second bottom plate (310) along the length direction. The initial thickness (D1) of the elastic pad (320) is greater than the thickness (D2) of the solid membrane (330). The overlapping section (231) is stacked on top of the elastic pad (320) and the solid membrane (330). The top plate assembly (400) includes a top plate (410) and a pressing slider (420). The top plate (410) is connected to the bottom shell (100). The top plate (410) is provided with a groove (410a). The position of the groove (410a) corresponds to the elastic pad (320) in the thickness direction. The pressing slider (420) is disposed in the groove (410a). The pressing slider (420) is used to press the overlapping section (231) and the elastic pad (320) so that the overlapping section (231) contacts the solid phase membrane (330).

2. The immunochromatographic test strip according to claim 1, characterized in that, The bottom shell (100) is provided with a mounting groove (100a), and the first test strip (200) and the second test strip (300) are disposed in the mounting groove (100a).

3. The immunochromatographic test strip according to claim 2, characterized in that, The bottom shell (100) and the top plate (410) are detachably connected.

4. The immunochromatographic test strip according to claim 1, characterized in that, The sample pad (220) and the conjugate pad (230) are both disposed on the upper surface of the first base plate (210), and the sample pad (220) and the conjugate pad (230) are connected; the overlapping section (231) of the conjugate pad (230) is stacked on top of the elastic pad (320) and the solid phase membrane (330).

5. The immunochromatographic test strip according to claim 1, characterized in that, The elastic pad (320) of the second test strip (300) is configured as a sponge pad or a rubber pad.

6. The immunochromatographic test strip according to claim 5, characterized in that, The initial thickness (D1) of the elastic pad (320) is 1.2 to 2 times the thickness (D2) of the solid phase film (330).

7. The immunochromatographic test strip according to claim 1, characterized in that, The top plate (410) is provided with a first observation hole (410b) and a second observation hole (410c), which are used to observe the T-line portion (331) and C-line portion (332) of the solid phase film (330), respectively. The lower surface of the top plate (410) is provided with a plurality of abutment limiting parts (411), which are spaced apart along the length direction. The abutment limiting parts (411) are used to press the first test strip (200) or the second test strip (300) into the bottom shell (100).

8. The immunochromatographic test strip according to any one of claims 1 to 7, characterized in that, The groove (410a) extends along the length direction, and the pressing slider (420) is slidably disposed in the groove (410a) along the length direction.

9. The immunochromatographic test strip according to claim 8, characterized in that, The top plate (410) is provided with an anti-detachment groove (410e); the pressing slider (420) is provided with a mating part (421), which is disposed in the anti-detachment groove (410e). The mating part (421) can slide relative to the anti-detachment groove (410e) in the length direction, and the thickness of the mating part (421) is less than the inner width of the anti-detachment groove (410e), so that the mating part (421) can float relative to the anti-detachment groove (410e) in the thickness direction.

10. The immunochromatographic test strip according to claim 9, characterized in that, The pressing slider (420) is provided with a positioning part (422), and the top plate (410) is provided with a positioning groove (410f); when the pressing slider (420) slides above the elastic pad (320), the positioning part (422) is inserted into the positioning groove (410f) along the length direction.

Images