An antibody incubation kit

Abstract

This utility model discloses an antibody incubation box, belonging to the technical field of laboratory equipment. It includes an upper cover and a lower box body, as well as a box body locking mechanism, a separating incubation mechanism, and a bottom hydrophobic mechanism. The upper cover and the front end of the lower box body are detachably connected via the box body locking mechanism. The separating incubation mechanism and the bottom hydrophobic mechanism are connected internally within the lower box body. The separating incubation mechanism and the bottom hydrophobic mechanism are interconnected. The separating incubation mechanism is used to simultaneously incubate multiple sets of PVDF membranes with antibodies. The bottom hydrophobic mechanism is used to reduce the amount of antibody solution used while ensuring experimental effectiveness. The separating incubation mechanism includes a placement plate and end plates. Two sets of end plates, symmetrically distributed front and back, are fixedly connected to the placement plate. The outer walls of the two sets of end plates are respectively slidably connected to the front and rear inner walls of the lower box body. The placement plate and end plates are connected to the bottom hydrophobic mechanism. Through this method, the amount of antibody used is greatly reduced while ensuring incubation effectiveness, thereby saving experimental costs.

Description

Technical Field

[0001] This utility model relates to the field of laboratory equipment technology, specifically to an antibody incubation box. Background Technology

[0002] Western blotting is a widely used protein detection technique in the biomedical field, and its antibody incubation step is crucial.

[0003] Currently, laboratories typically use self-sealing bags or flat-bottomed incubation boxes for antibody incubation. These traditional methods have the following significant drawbacks: First, the antibody consumption is too high. To ensure the incubation effect, sufficient antibody solution must be injected to completely cover the entire membrane. Incubating a single standard membrane often requires 5-10 mL of antibody working solution, which is costly for expensive primary antibodies. On the other hand, reducing the incubation volume can easily lead to uneven membrane coverage, drying, or insufficient reaction, affecting experimental reproducibility. Second, it is inconvenient to operate when incubating multiple samples. Ordinary flat-bottomed incubation boxes have poor stacking stability and are prone to sliding and tipping during shaker oscillation, posing a risk of solution leakage and sample confusion.

[0004] Based on this, the present invention designs an antibody incubation box to solve the above problems. Utility Model Content

[0005] In view of the above-mentioned shortcomings of the existing technology, the present invention provides an antibody incubation box.

[0006] To achieve the above objectives, this utility model provides the following technical solution: An antibody incubation box includes an upper cover and a lower box body, and also includes a box body locking mechanism, a separating incubation mechanism, and a bottom hydrophobic mechanism. The upper cover and the front end of the lower box body are detachably connected through the box body locking mechanism. The separating incubation mechanism and the bottom hydrophobic mechanism are connected inside the lower box body. The separating incubation mechanism and the bottom hydrophobic mechanism are interconnected. The separating incubation mechanism is used to simultaneously incubate multiple sets of PVDF membranes with antibodies, and the bottom hydrophobic mechanism is used to reduce the amount of antibody solution used while ensuring the effectiveness of the experiment. The incubation mechanism includes a placement plate and end plates. Two sets of end plates, symmetrically distributed front and back, are fixedly connected to the placement plate. The outer walls of the two sets of end plates are respectively limited and slidably connected to the front and rear inner walls of the lower box. The placement plate and end plates are connected to the bottom hydrophobic mechanism.

[0007] Furthermore, the box locking mechanism includes an upper locking block, a locking shaft, and a lower locking block. The upper locking block is hinged to the front end of the upper box cover via the locking shaft, and the lower locking block is fixedly connected to the front end of the lower box body. A locking protrusion is fixedly connected to the upper wall of the lower end of the upper locking block, and a locking groove is provided at the lower end of the locking shaft to engage with the locking protrusion.

[0008] Furthermore, the placement plate has multiple incubation groups that are equidistantly distributed front to back. Each incubation group consists of multiple incubation wells that are equidistantly distributed left to right. Each incubation well penetrates the upper and lower walls of the placement plate and is used for antibody incubation.

[0009] Furthermore, the bottom hydrophobic mechanism includes side plates and a hydrophobic membrane. Two sets of side plates, symmetrically distributed on the left and right, are fixedly connected to the upper part of the bottom surface of the lower box. The side plates are provided with hydrophobic placement grooves for inserting into the hydrophobic membrane. The lower end of the placement plate is provided with a hydrophobic clearance groove for accommodating the hydrophobic membrane. The outer walls of the end plate and the placement plate are respectively limited and slidably connected to the inner walls of the side plates.

[0010] Furthermore, a nylon ring is fixedly connected to the lower end of the incubation well to prevent the antibody solution from flowing out of the incubation well.

[0011] Furthermore, there are fixing components connecting the upper lid and the lower box body.

[0012] Furthermore, the fixing components include fixing blocks, with four sets of fixing blocks fixedly connected to the four corners of the upper box cover, and four sets of fixing slots for inserting into the fixing blocks at the four corners of the lower box body.

[0013] Furthermore, the upper connection of the fixing block is an arc-shaped structure with a guiding function.

[0014] Furthermore, both the upper locking block and the locking pivot are made of plastic.

[0015] Compared with the prior art, the advantages of this utility model are as follows: 1. The antibody solution is added to the incubation well with a pipette, and then a PVDF membrane is added for incubation. Since the lower end is fixed with a hydrophobic membrane, the antibody solution will only be on the PVDF membrane. Therefore, only a very small amount of antibody solution needs to be added to meet the incubation requirements, which reduces the amount of antibody used and greatly saves the cost of the experiment while ensuring the incubation effect. 2. The placement plate has multiple sets of incubation wells, allowing multiple parallel experiments to be conducted simultaneously; 3. When more than one set of incubation boxes needs to be incubated, stacking multiple sets of incubation boxes and inserting the fixing blocks into the fixing slots strengthens the fixing effect between different incubation boxes, making the stacking of multiple sets of incubation boxes more stable. This makes the incubation boxes less likely to collapse or tip over when the refrigerator door is opened or when the shaker vibrates, which is beneficial to the stability of the incubation process and the smooth progress of the experiment. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a perspective view of an antibody incubation box according to the present invention; Figure 2 This utility model provides a three-dimensional view of an antibody incubation box when opened. Figure 1 ; Figure 3 This is a front view of an antibody incubation box of this utility model when the lid is opened; Figure 4 This utility model provides a three-dimensional view of an antibody incubation box when opened. Figure 2 ; Figure 5 This is a schematic diagram of the structure of an antibody incubation box according to the present invention; Figure 6 This is a structural schematic diagram of Embodiment 3 of the present invention.

[0018] The labels in the diagram represent: 1. Upper lid; 2. Lower body; 3. Body hinge; 4. Upper locking block; 5. Locking hinge; 6. Lower locking block; 7. Placement plate; 8. Incubation hole; 9. Hydrophobic clearance groove; 10. Side plate; 11. Hydrophobic placement groove; 12. Hydrophobic membrane; 13. End plate; 14. Fixing block; 15. Fixing groove. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0020] The terms "left," "right," "front," "back," "up," and "down" used in the following description refer to the orientation from the perspective of the front view.

[0021] Example 1: In some embodiments, please refer to the accompanying drawings. Figures 1-6An antibody incubation box includes an upper cover 1 and a lower box body 2, and also includes a box body locking mechanism, a separating incubation mechanism and a bottom hydrophobic mechanism. The front ends of the upper cover 1 and the lower box body 2 are detachably connected through the box body locking mechanism. The separating incubation mechanism and the bottom hydrophobic mechanism are connected inside the lower box body 2. The separating incubation mechanism and the bottom hydrophobic mechanism are interconnected.

[0022] The upper lid 1 and the lower box body 2 are hinged at their rear ends via a box body pivot 3.

[0023] The box locking mechanism includes an upper locking block 4, a locking shaft 5, and a lower locking block 6. The upper locking block 4 is hinged to the front end of the upper box cover 1 via the locking shaft 5, and the lower locking block 6 is fixedly connected to the front end of the lower box body 2. A locking protrusion is fixedly connected to the upper wall of the lower end of the upper locking block 4, and a locking groove is provided at the lower end of the locking shaft 5 to engage with the locking protrusion. Both the upper locking block 4 and the locking shaft 5 are made of plastic. When the lower end of the upper box cover 1 abuts against the upper end of the lower box body 2, the upper locking block 4 is rotated so that the locking protrusion engages in the locking groove, thereby fixing the upper box cover 1 and the lower box body 2.

[0024] In this utility model, with the cooperation of the box body rotating shaft 3, the upper box cover 1 is rotated so that the lower end of the upper box cover 1 abuts against the upper end of the lower box body 2. With the cooperation of the locking rotating shaft 5, the upper locking block 4 is rotated so that the locking protrusion on the upper locking block 4 is engaged in the locking groove on the lower locking block 6, thereby fixing the upper box cover 1 and the lower box body 2.

[0025] Example 2: In some embodiments, such as Figure 5 As shown, in a preferred embodiment of the present invention, the separation incubation mechanism includes a placement plate 7 and an end plate 13. Two sets of end plates 13, symmetrically distributed front and back, are fixedly connected to the placement plate 7. The outer walls of the two sets of end plates 13 are respectively limited and slidably connected to the front and back inner walls of the lower box 2. The placement plate 7 and the end plates 13 are connected to the bottom hydrophobic mechanism.

[0026] The placement plate 7 has multiple incubation groups that are equidistantly distributed front to back. Each incubation group consists of multiple incubation wells 8 that are equidistantly distributed left to right. Each incubation well 8 penetrates the upper and lower walls of the placement plate 7. The incubation wells 8 are used for antibody incubation. The number of incubation wells 8 determines how many parallel experiments can be performed simultaneously in one antibody incubation box.

[0027] The bottom hydrophobic mechanism includes a side plate 10 and a hydrophobic membrane 12. Two sets of side plates 10, symmetrically distributed on the left and right, are fixedly connected to the upper part of the bottom surface of the lower box 2. A hydrophobic placement groove 11 is provided on the side plate 10 to insert into the hydrophobic membrane 12. A hydrophobic clearance groove 9 for accommodating the hydrophobic membrane 12 is provided at the lower end of the placement plate 7. The outer walls of the end plate 13 and the placement plate 7 are respectively limited and slidably connected to the inner wall of the side plate 10.

[0028] A nylon ring is fixedly connected to the lower end of the incubation well 8. The nylon ring is used to prevent the antibody solution from flowing out of the incubation well 8.

[0029] In this invention, the hydrophobic membrane 12 is inserted into the hydrophobic placement groove 11 of the end plate 13, and the placement plate 7 and the end plate 13 are placed into the lower box 2, so that the outer wall of the end plate 13 is in contact with the front and rear inner walls of the lower box 2, and the outer walls of the placement plate 7 and the end plate 13 are in contact with the inner wall of the side plate 10. The antibody solution is added to the incubation well 8 with a pipette, and then the PVDF membrane is added for incubation. Since the hydrophobic membrane 12 is fixed at the lower end, the antibody solution will only be on the PVDF membrane. Therefore, only a very small amount of antibody solution needs to be added to meet the incubation requirements, reducing the amount of antibody used. While ensuring the incubation effect, the experimental cost is greatly reduced.

[0030] Example 3: In some embodiments, such as Figure 6 As shown, in a preferred embodiment of this utility model, a fixing component is connected to the upper cover 1 and the lower box body 2.

[0031] The fixing component includes fixing blocks 14. Four sets of fixing blocks 14 are fixedly connected to the four corners of the upper end of the upper box cover 1. Four sets of fixing slots 15 are provided at the four corners of the lower end of the lower box body 2 to be inserted into the fixing blocks 14.

[0032] The upper connection of the fixing block 14 is an arc-shaped structure with a guiding function.

[0033] In this invention, the upper lid 1 and lower body 2 of the incubation box containing the antibody solution and PVDF membrane are fixed by the upper locking block 4 and the lower locking block 6. After fixing, the box can be placed on a shaker for mixing, or placed in a refrigerator and incubated overnight at about 4 degrees Celsius before proceeding to the next experiment. When more than one set of incubation boxes needs to be shaken and frozen for incubation, multiple sets of incubation boxes can be stacked so that the fixing block 14 is inserted into the fixing slot 15. This strengthens the fixing effect between different incubation boxes, making the stacking of multiple sets of incubation boxes more stable. This makes the incubation boxes less likely to collapse during shaking or when opening and closing the refrigerator door, which is beneficial to the stability of the incubation process and the smooth progress of the experiment.

[0034] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. An antibody incubation box, comprising an upper lid (1) and a lower body (2), characterized in that: It also includes a box locking mechanism, a separation incubation mechanism and a bottom hydrophobic mechanism. The front ends of the upper box cover (1) and the lower box body (2) are detachably connected through the box locking mechanism. The lower box body (2) is internally connected to the separation incubation mechanism and the bottom hydrophobic mechanism. The separation incubation mechanism and the bottom hydrophobic mechanism are interconnected. The separation incubation mechanism is used to incubate multiple PVDF membranes with antibodies at the same time. The bottom hydrophobic mechanism is used to reduce the amount of antibody solution used while ensuring the effectiveness of the experiment. The separate incubation mechanism includes a placement plate (7) and an end plate (13). Two sets of end plates (13) are symmetrically distributed front and back and are fixedly connected to the placement plate (7). The outer walls of the two sets of end plates (13) are respectively limited and slidably connected to the front and rear inner walls of the lower box (2). The placement plate (7) and the end plate (13) are connected to the bottom hydrophobic mechanism.

2. The antibody incubation kit according to claim 1, characterized in that, The box locking mechanism includes an upper locking block (4), a locking shaft (5) and a lower locking block (6). The upper locking block (4) is hinged to the front end of the upper box cover (1) through the locking shaft (5). The lower locking block (6) is fixedly connected to the front end of the lower box body (2). A locking protrusion is fixedly connected to the upper wall of the lower end of the upper locking block (4). A locking groove is opened at the lower end of the locking shaft (5) to engage with the locking protrusion.

3. The antibody incubation kit according to claim 1, characterized in that, The placement plate (7) has multiple incubation groups that are equidistantly distributed front to back. Each incubation group consists of multiple incubation wells (8) that are equidistantly distributed left to right. Each incubation well (8) penetrates the upper and lower walls of the placement plate (7) and is used for antibody incubation.

4. The antibody incubation kit according to claim 3, characterized in that, The bottom hydrophobic mechanism includes a side plate (10) and a hydrophobic membrane (12). Two sets of side plates (10) are symmetrically distributed on the left and right sides and are fixedly connected to the upper part of the bottom surface of the lower box (2). A hydrophobic placement groove (11) for inserting into the hydrophobic membrane (12) is provided on the side plate (10). A hydrophobic clearance groove (9) for accommodating the hydrophobic membrane (12) is provided at the lower end of the placement plate (7). The outer walls of the end plate (13) and the placement plate (7) are respectively limited and slidably connected to the inner wall of the side plate (10).

5. The antibody incubation kit according to claim 4, characterized in that, A nylon ring is fixedly connected to the lower end of the incubation well (8). The nylon ring is used to prevent the antibody solution from flowing out of the incubation well (8).

6. The antibody incubation kit according to claim 1, characterized in that, The upper lid (1) and the lower body (2) are connected to a fixing component.

7. The antibody incubation kit according to claim 6, characterized in that, The fixing components include fixing blocks (14), four sets of fixing blocks (14) are fixedly connected to the four corners of the upper end of the upper box cover (1), and four sets of fixing slots (15) are opened at the four corners of the lower end of the lower box body (2) to be inserted into the fixing blocks (14).

8. The antibody incubation kit according to claim 7, characterized in that, The upper end of the fixing block (14) is an arc-shaped structure with a guiding function.

9. The antibody incubation kit according to claim 1, characterized in that, Both the upper locking block (4) and the locking pivot (5) are made of plastic.

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