A cryopreservation device for NK cells

Abstract

The utility model relates to the field of biological medical technology discloses a kind of freeze storage devices for NK cell, including freeze storage box, the inner chamber of freeze storage box is equipped with several placement grooves of placing test tube, the inner chamber of freeze storage box is equipped with connecting groove, the surface of connecting groove is slidably provided with connecting column, the surface of connecting column is fixed with box cover, the surface of freeze storage box is provided with adsorption mechanism, the inner chamber of freeze storage box is equipped with several percussion mechanism used in cooperation with placement groove;The utility model can be adsorbed in specified position under the action of adsorption mechanism when freeze storage box is used, so that freeze storage box tends to be stable, so that the placement and pulling out of NK cell test tube are facilitated, in addition, the test tube after freezing can be knocked under the action of percussion mechanism, so that the connection between test tube and freeze storage box due to freezing is avoided, and the pulling out of test tube is affected.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, specifically to a cryopreservation device for NK cells. Background Technology

[0002] NK cells, or natural killer cells, are a type of innate lymphocyte-like cell that can spontaneously kill target cells without prior antigen sensitization, hence the name natural killer cells. Cell cryopreservation is a technique that places cells in a low-temperature environment to reduce cell metabolism for long-term storage. Cell cryopreservation is one of the main methods of cell preservation and plays an important role in cell seed preservation. For long-term storage of NK cells, cell cryopreservation devices are required.

[0003] When cryopreserving NK cells, cryopreservation boxes are needed in conjunction with test tubes. During the cryopreservation process, the cryopreservation box is usually placed directly in a designated position, and then the test tubes are moved between the box and the cryopreservation box. However, since the position of the cryopreservation box is not fixed, it is inconvenient for staff to insert or remove the test tubes, which causes some trouble for the staff. Utility Model Content

[0004] The purpose of this invention is to provide a cryopreservation device for NK cells to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cryopreservation device for NK cells, comprising a cryopreservation box, the inner cavity of which is provided with a plurality of placement slots for placing test tubes, the inner cavity of which is provided with a connecting slot, a connecting post slidably disposed on the surface of the connecting slot, a box lid fixed on the surface of the connecting post, an adsorption mechanism disposed on the surface of the cryopreservation box, and the inner cavity of which is provided with a plurality of tapping mechanisms for use with the placement slots.

[0006] Preferably, the adsorption mechanism includes a suction cup disposed at the bottom of the cryopreservation box, one end of the suction cup being connected to a connecting tube, a sealing block being fixed in the inner cavity of the connecting tube, a plurality of sliding rods being fixed on the surface of the sealing block, a movable blocking block being slidably disposed on the surface of the sliding rods, a first spring being fixed on one side of the surface of the movable blocking block, a connecting block being fixed at the other end of the sliding rod, the other end of the connecting block being fixed to the inner cavity of the connecting tube, and the other end of the first spring being fixed to the surface of the connecting block.

[0007] Preferably, a pull rod is fixed to one side of the surface of the movable block, and a pull block is fixed to the other end of the pull rod, wherein the outer diameter of the pull block is larger than the outer diameter of the connecting pipe.

[0008] Preferably, the tapping mechanism includes a plurality of limiting rods fixed to the inner cavity of the cryopreservation box, a tapping block is slidably disposed on the surface of the limiting rod, a second spring is fixed to one side of the surface of the tapping block, the other end of the second spring is fixed to the inner cavity of the cryopreservation box, a connecting rod is fixed to the surface of the tapping block, and a pressure block is fixed to the other end of the connecting rod.

[0009] Preferably, the inner cavity of the cryopreservation box is provided with a plurality of limiting grooves, and the connecting rod slides on the surface of the limiting grooves.

[0010] Preferably, the material of the striking block is rubber.

[0011] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0012] When this invention is used in a cryopreservation box, the adsorption mechanism allows the cryopreservation box to be adsorbed at a designated position, thereby stabilizing the cryopreservation box and facilitating the placement and removal of NK cell test tubes. In addition, the tapping mechanism allows the frozen test tubes to be tapped, thereby preventing the test tubes from becoming stuck to the cryopreservation box due to freezing, which would affect the removal of the test tubes. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0014] Figure 2 This is a three-dimensional structural diagram of the cryopreservation box and its lid after separation in this utility model;

[0015] Figure 3 This is a partial three-dimensional structural diagram of the present invention;

[0016] Figure 4 This is a partial three-dimensional structural diagram of the present invention;

[0017] Figure 5 This is a partial three-dimensional structural diagram of the present invention;

[0018] Figure 6 This utility model Figure 5 A magnified schematic diagram of the local three-dimensional structure at point A.

[0019] In the diagram: 1. Cryopreservation box; 2. Placement slot; 3. Connecting slot; 4. Connecting column; 5. Box lid; 6. Adsorption mechanism; 61. Suction cup; 62. Connecting tube; 63. Sealing block; 64. Sliding rod; 65. Moving blocking block; 66. First spring; 67. Connecting block; 7. Pull rod; 8. Pull block; 9. Tapping mechanism; 91. Limiting rod; 92. Tapping block; 93. Second spring; 94. Connecting rod; 95. Pressure block; 10. Limiting slot. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figure 1-6 As shown, a cryopreservation device for NK cells includes a cryopreservation box 1, an inner cavity of which is provided with a plurality of placement slots 2 for placing test tubes, a connecting slot 3 in the inner cavity of the cryopreservation box 1, a connecting post 4 slidably disposed on the surface of the connecting slot 3, a box cover 5 fixed on the surface of the connecting post 4, an adsorption mechanism 6 disposed on the surface of the cryopreservation box 1, and a plurality of tapping mechanisms 9 in the inner cavity of the cryopreservation box 1 for use with the placement slots 2.

[0022] The adsorption mechanism 6 includes a suction cup 61 disposed at the bottom of the cryopreservation box 1. One end of the suction cup 61 is connected to a connecting tube 62, which is disposed at the bottom of the inner cavity of the cryopreservation box 1. A sealing block 63 is fixed inside the inner cavity of the connecting tube 62. Several sliding rods 64 are fixed to the surface of the sealing block 63. A movable blocking block 65 is slidably disposed on the surface of the sliding rods 64. A first spring 66 is fixed to one side of the surface of the movable blocking block 65. A connecting block 67 is fixed to the other end of the sliding rods 64. The other end of the connecting block 67 is fixed to the inner cavity of the connecting tube 62. The other end of the first spring 66 is fixed to the surface of the connecting block 67. In this embodiment, the suction cup 61... The arrangement of the connecting tube 62, sealing block 63, sliding rod 64, movable blocking block 65, first spring 66, and connecting block 67 allows the movable blocking block 65 to adhere to the surface of the sealing block 63 under the action of the first spring 66, thereby achieving the purpose of closing the connecting tube 62. When the suction cup 61 adheres to the smooth surface, the cryopreservation box 1 can be stably positioned in the designated area under the action of the suction cup 61, making it easier for staff to place or remove test tubes in the placement slot 2. In addition, when the movable blocking block 65 moves away from the sealing block 63, air can enter the suction cup 61, thereby disabling the use of the suction cup 61.

[0023] A pull rod 7 is fixed to one side of the surface of the movable block 65, and a pull block 8 is fixed to the other end of the pull rod 7. The outer diameter of the pull block 8 is larger than the outer diameter of the connecting pipe 62. In this embodiment, by setting the pull rod 7 and the pull block 8, when the operator needs to move the movable block 65, the operator can pass through the force point under the action of the pull rod 7 and the pull block 8, so as to facilitate the operator to move the movable block 65, thereby achieving the purpose of cooperating with the adsorption mechanism 6.

[0024] The tapping mechanism 9 includes several limiting rods 91 fixed to the inner cavity of the cryopreservation box 1. A tapping block 92 is slidably disposed on the surface of the limiting rods 91. A second spring 93 is fixed to one side of the surface of the tapping block 92, and the other end of the second spring 93 is fixed to the inner cavity of the cryopreservation box 1. A connecting rod 94 is fixed to the surface of the tapping block 92, and a pressure block 95 is fixed to the other end of the connecting rod 94. In this embodiment, through the arrangement of the limiting rods 91, tapping block 92, second spring 93, connecting rod 94 and pressure block 95, when the pressure block 95 is under force, the tapping block 92 can slide on the surface of the limiting rods 91 under the action of the connecting rod 94. After the control of the pressure block 95 is released, the tapping block 92 can act on the surface of the test tube under the action of the second spring 93, thereby achieving the purpose of tapping it, loosening the test tube, and making it easier to pull out.

[0025] The inner cavity of the cryopreservation box 1 is provided with several limiting grooves 10. The connecting rod 94 slides on the surface of the limiting grooves 10. In this embodiment, the limiting grooves 10 provide space for the sliding of the connecting rod 94 and provide a limit for it, thereby improving the stability of the connecting rod 94 when it slides.

[0026] The striking block 92 is made of rubber. In this embodiment, this design makes the impact between the striking block 92 and the test tube a soft impact, reducing the damage caused by the striking block 92 to the test tube.

[0027] Working Principle: When performing NK cell cryopreservation, if the operator needs to place the cell tubes in the placement slot 2, they can first press the cryopreservation box 1 onto the smooth table surface using the suction cup 61. The suction cup 61 then allows the cryopreservation box 1 to adhere to the table surface, improving its stability and facilitating the placement of the test tubes and the insertion / removal of the lid 5, thus enhancing the device's practicality. After cryopreservation, if the operator needs to remove the cell tubes, they can use the designated pressure block 95 to press down on them. Under the action of the connecting rod 94, the striking block 92 descends. After releasing the pressure block 95, the second spring 93 allows the striking block 92 to tap the test tubes, loosening them and preventing the test tubes from being difficult to remove due to cryopreservation, further improving the device's practicality.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cryopreservation device for NK cells, comprising a cryopreservation box (1), characterized in that: The cryopreservation box (1) has several placement slots (2) for placing test tubes in its inner cavity. The cryopreservation box (1) has a connecting slot (3) in its inner cavity. A connecting post (4) is slidably disposed on the surface of the connecting slot (3). A box cover (5) is fixed on the surface of the connecting post (4). An adsorption mechanism (6) is disposed on the surface of the cryopreservation box (1). The cryopreservation box (1) has several tapping mechanisms (9) that are used in conjunction with the placement slots (2) in its inner cavity.

2. The cryopreservation device for NK cells according to claim 1, characterized in that: The adsorption mechanism (6) includes a suction cup (61) disposed at the bottom of the cryopreservation box (1). One end of the suction cup (61) is connected to a connecting tube (62). A sealing block (63) is fixed in the inner cavity of the connecting tube (62). Several sliding rods (64) are fixed on the surface of the sealing block (63). A movable blocking block (65) is slidably disposed on the surface of the sliding rod (64). A first spring (66) is fixed on one side of the surface of the movable blocking block (65). A connecting block (67) is fixed at the other end of the sliding rod (64). The other end of the connecting block (67) is fixed to the inner cavity of the connecting tube (62). The other end of the first spring (66) is fixed to the surface of the connecting block (67).

3. The cryopreservation device for NK cells according to claim 2, characterized in that: A pull rod (7) is fixed to one side of the surface of the movable block (65), and a pull block (8) is fixed to the other end of the pull rod (7). The outer diameter of the pull block (8) is larger than the outer diameter of the connecting pipe (62).

4. The cryopreservation device for NK cells according to claim 1, characterized in that: The tapping mechanism (9) includes several limiting rods (91) fixed to the inner cavity of the cryopreservation box (1). A tapping block (92) is slidably disposed on the surface of the limiting rod (91). A second spring (93) is fixed on one side of the surface of the tapping block (92). The other end of the second spring (93) is fixed to the inner cavity of the cryopreservation box (1). A connecting rod (94) is fixed on the surface of the tapping block (92). A pressure block (95) is fixed on the other end of the connecting rod (94).

5. A cryopreservation device for NK cells according to claim 4, characterized in that: The inner cavity of the cryopreservation box (1) is provided with several limiting grooves (10), and the connecting rod (94) slides on the surface of the limiting grooves (10).

6. A cryopreservation device for NK cells according to claim 4, characterized in that: The striking block (92) is made of rubber.

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