A device for rapid condensation collection of exhaled breath for MALDI mass spectrometry

Abstract

The utility model discloses a kind of for the exhaled air quick condensation collection device of MALDI mass spectrum detection, belong to biomedical detection technical field, it includes condensing pedestal and detachable cover, the condensing pedestal upper surface is provided with chip groove, the condensing pedestal upper surface is located chip groove outside and is provided with the mounting groove for installing detachable cover, the condensing pedestal bottom is provided with condensing component placing groove, silicon nanowire chip is provided in the chip groove, condensing component is provided in the condensing component placing groove, detachable cover is communicated with air nozzle on, detachable cover is detachably mounted in mounting groove. The exhaled air quick condensation collection device can be carried out exhaled air noninvasive collection, after collection, it can directly carry out quick mass spectrum detection, shorten gas sample collection, analysis time, improve detection efficiency.

Description

Technical Field

[0001] This invention belongs to the field of biomedical detection technology, specifically relating to a rapid condensation and collection device for exhaled breath used in MALDI mass spectrometry detection. Background Technology

[0002] Respiratory diseases have become one of the leading causes of death in recent years. These include a variety of common and frequently occurring diseases such as asthma, tracheitis, bronchitis, chronic obstructive pulmonary disease (COPD), tuberculosis, and lung cancer. With the aging population and other factors, the incidence and mortality rates of respiratory diseases are increasing year by year. Therefore, developing rapid and accurate methods for the diagnosis and health management of respiratory diseases is of great significance.

[0003] Using exhaled breath for diagnosis of respiratory and other diseases has a long history because it contains a wealth of substances related to human metabolism and gut microbiota. It reflects not only systemic, endogenous metabolites produced during physiological processes, but also information from inhaled air, ingested food, and beverages. Studies have shown that biochemical testing of exhaled breath samples provides richer and more accurate information than single nasal or pharyngeal swab samples, significantly reducing false negatives in nucleic acid testing. Furthermore, exhaled breath collection and testing are non-invasive, convenient, and repeatable. Compared to traditional biochemical tests such as blood tests, urine tests, imaging studies, and tissue sections, exhaled breath and its condensate testing offer significant advantages in terms of testing frequency, sample acquisition, and cost.

[0004] Currently, mass spectrometry is the preferred method for detecting metabolites in human exhaled breath samples, with GC-MS being the most comprehensive and sensitive method for characterizing volatile components in exhaled gas. Its exploration of fingerprint patterns for highly volatile or semi-volatile organic compounds in exhaled gas is quite advanced. However, GC-MS still has some limitations in analyzing metabolites in exhaled breath samples. Gas chromatography requires certain sample processing, including gas sample collection and pre-enrichment, as well as derivatization and desalting steps. Gas chromatography analysis is only suitable for indirect sampling and not for real-time analysis, and the detection time is long (a typical GC-MS analysis may take up to an hour). Therefore, GC-MS is not suitable for rapid clinical detection and diagnosis. Furthermore, GC-MS detection involves the loss and degradation of analytes, especially active or heat-sensitive metabolites.

[0005] It is evident that precise instrumental analysis of the complex components of exhaled gas requires preprocessing steps such as gas sample collection and enrichment, as well as lengthy detection and analysis procedures. These factors limit the application of exhaled gas in clinical diagnosis and precision medicine.

[0006] Therefore, non-invasive collection and rapid mass spectrometry detection of human exhaled air samples to shorten the gas sample collection and analysis time and improve detection efficiency is an urgent problem to be solved. Utility Model Content

[0007] The purpose of this invention is to provide a rapid condensation and collection device for exhaled breath for MALDI mass spectrometry detection, thereby solving the problem of low detection efficiency in existing technologies.

[0008] The technical solution adopted in this utility model is as follows:

[0009] A rapid exhaled breath condensation collection device for MALDI mass spectrometry detection includes a condensation base and a detachable cover. The upper surface of the condensation base is provided with a chip slot, and the upper surface of the condensation base outside the chip slot is provided with a mounting slot for installing the detachable cover. The bottom of the condensation base is provided with a condensation component placement slot. A silicon nanowire chip is disposed in the chip slot, and a condensation component is disposed in the condensation component placement slot. An air nozzle is connected to the detachable cover, and the detachable cover can be detachably installed in the mounting slot.

[0010] Furthermore, the silicon nanowire chip surface is provided with a fluorinated ethylene propylene copolymer modification layer.

[0011] Furthermore, the condensation component is a condensation pad or frozen ice.

[0012] Furthermore, the detachable cover is provided with an exhaust port.

[0013] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0014] 1. This invention constructs an exhaled breath condensation collection device for use with MALDI mass spectrometry. Exhaled breath is introduced into the device through a detachable nozzle. The exhaled breath is condensed into liquid by a condensation component at the bottom of the condensation base and collected on the surface of a silicon nanowire chip. After collection, the dried silicon nanowire chip is removed for subsequent direct MALDI mass spectrometry detection. This device significantly shortens the exhaled breath sample collection time, reduces the required sample volume, improves detection efficiency, avoids dizziness caused by prolonged continuous exhalation, and greatly enhances the collection experience.

[0015] 2. In this invention, exhaled gas and its condensate samples are directly collected on the surface of a silicon nanowire chip. The silicon nanowire chip enhances the capture ability of target analytes and assists in the desorption ionization process. Through its surface nanostructure, it adsorbs, captures, and enriches biochemical molecules in exhaled gas, avoiding sample processing and transfer steps. The silicon nanowire chip containing the collected exhaled gas sample is then directly subjected to MALDI mass spectrometry detection via a target plate, reducing target analyte loss and interference, and simplifying the detection process. Furthermore, due to the good stability and repeatability of the silicon nanowire chip, this device has the potential to become a home health management product. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort, wherein:

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a top view of the condenser base of this utility model;

[0019] Figure 3 This is a bottom view of the condenser base of this utility model;

[0020] The markings in the diagram are: 1-condenser base, 2-removable cover, 3-chip slot, 4-mounting slot, 5-condenser component placement slot, 6-gas nozzle, 7-exhaust port. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0023] It should be noted that the labels and letters in the following figures represent similar items, therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0024] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are merely for the purpose of simplifying the description of this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0025] Furthermore, terms such as "horizontal" and "vertical" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," not that the structure must be completely horizontal, but can be slightly tilted.

[0026] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0027] Matrix-assisted laser desorption / ionization-time of flight mass spectrometry (MALDI-TOF MS) offers significant advantages over GC-MS, including faster detection speed, higher throughput, and lower cost. As a soft ionization technique, it possesses unique advantages in detecting and identifying metabolites in complex biological samples and in performing extensive qualitative and quantitative analysis of metabolites. Surface assistance can further enhance the detection performance of MALDI-TOF MS, a technique known as surface-assisted laser desorption / ionization (SALDI). The advantage of SALDI lies in the fact that the inorganic matrix on the surface does not ionize under laser irradiation; instead, it absorbs laser energy, rapidly increasing the local temperature and promoting the desorption and ionization process of the analyte molecules.

[0028] This application constructs an exhaled breath condensation collection device for use with MALDI mass spectrometry, enabling MALDI mass spectrometry to be applied to rapid exhaled breath detection and expanding the application capabilities of mass spectrometry technology in clinical testing.

[0029] Refer to the instruction manual. Figure 1-3 ,

[0030] A rapid exhaled breath condensation collection device for MALDI mass spectrometry detection includes a condensation base 1 and a detachable cover 2. The upper surface of the condensation base 1 is provided with a chip slot 3, and the upper surface of the condensation base 1, outside the chip slot 3, is provided with a mounting slot 4 for installing the detachable cover 2. The bottom of the condensation base 1 is provided with a condensation component placement slot 5. A silicon nanowire chip is disposed in the chip slot 3, and a condensation component is disposed in the condensation component placement slot 5. An air nozzle 6 is connected to the detachable cover 2, and the detachable cover 2 is detachably installed in the mounting slot 4.

[0031] In this invention, a silicon nanowire chip is placed in the chip slot of a condensation base. A detachable cover is then installed on the condensation base via a mounting slot. A condensation pad or ice is placed at the bottom of the condensation base. The subject exhales through the nozzle of the detachable cover. The exhaled gas condenses directly into liquid due to the low temperature generated by the condensation pad or ice at the bottom of the condensation base, and is collected on the surface of the silicon nanowire chip. After collection, the dried silicon nanowire chip is removed for subsequent direct MALDI mass spectrometry analysis. This device significantly shortens the collection time of exhaled gas samples, reduces the required sample volume, avoids dizziness caused by prolonged continuous exhalation, and greatly improves the collection experience.

[0032] The test samples were analyzed using a Brookdalen UltrafleXtreme MALDI-TOF / TOF instrument with a 355nm Nd:YAG laser beam. Pulsed ion extraction and laser parameters were set to 120ns and 4_large, respectively. Mass spectra were obtained by superimposing 500 and 1000 laser bombardments in reflection and linear modes, respectively. All detections were performed in triplicate. Comparisons of sample spectra within and between batches showed consistency, indicating good stability. After storing the dried chip for different periods (up to 21 days), further detection and comparison showed no significant changes in the approximate distribution and number of characteristic mass spectrometry peaks, demonstrating the chip's good stability and repeatability in mass spectrometry. Therefore, this device can be used as a home health management product.

[0033] In a preferred embodiment, the surface of the silicon nanowire chip is provided with a fluorinated ethylene propylene copolymer modification layer.

[0034] In this embodiment, the silicon nanowire chip with a fluorinated ethylene propylene copolymer modified layer can improve the capture ability of analytes in exhaled air and its condensate, while also assisting the desorption ionization process to form better mass spectrometry detection results.

[0035] In a preferred embodiment, the condensation component is a condensation pad or frozen ice.

[0036] In this embodiment, the condensation pad and frozen ice are readily available, making it easier for the device to be applied to home health management products.

[0037] In a preferred embodiment, the detachable cover 2 is provided with an exhaust port 7.

[0038] In this embodiment, once the device is assembled, the exhaust port facilitates the discharge of the initial gas inside the device, making it easier for the subject to exhale the gas into the device.

[0039] The above description constitutes an embodiment of this utility model. The foregoing descriptions are preferred embodiments of this utility model. Unless there is a clear contradiction between the preferred embodiments or a premise based on a particular preferred embodiment, the preferred embodiments can be arbitrarily combined and used. The embodiments and specific parameters described are merely for clearly illustrating the verification process of the utility model and are not intended to limit the patent protection scope of this utility model. The patent protection scope of this utility model is still determined by its claims. Similarly, any equivalent structural changes made based on the description and drawings of this utility model should also be included within the protection scope of this utility model.

Claims

1. A rapid exhaled breath condensation and collection device for MALDI mass spectrometry detection, characterized in that, The device includes a condenser base (1) and a removable cover (2). The upper surface of the condenser base (1) is provided with a chip slot (3). The upper surface of the condenser base (1) is provided with an installation slot (4) for installing the removable cover (2) outside the chip slot (3). The bottom of the condenser base (1) is provided with a condenser component placement slot (5). A silicon nanowire chip is provided in the chip slot (3). A condenser component is provided in the condenser component placement slot (5). An air nozzle (6) is connected to the removable cover (2). The removable cover (2) can be detachably installed in the installation slot (4).

2. The exhaled breath rapid condensation collection device for MALDI mass spectrometry detection according to claim 1, characterized in that, The silicon nanowire chip surface is provided with a fluorinated ethylene propylene copolymer modification layer.

3. The exhaled breath rapid condensation collection device for MALDI mass spectrometry detection according to claim 1, characterized in that, The condensation component is a condensation pad or frozen ice.

4. The rapid condensation and collection device for exhaled breath in MALDI mass spectrometry detection according to claim 1, characterized in that, The detachable cover (2) is provided with an exhaust port (7).

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