A tangential flow filtration device for RNA lipid nanoparticle preparation

Abstract

The utility model relates to biological pharmacy equipment technical field, a tangential flow filtration device for RNA lipid nano particle preparation, including the conveying pipe, the outside close bottom fixed mounting of conveying pipe has the discharge pipe, the inside of conveying pipe is symmetrical formula and is opened with the circular clamping slot, the circular clamping slot swing joint has the ring clamping block, the inboard fixed mounting of ring clamping block has the arc plate, the inside of conveying pipe is circularly rotatory and is provided with the rotating mechanism that carries out self -cleaning operation after filtering RNA lipid nano particle, the upper and lower both ends of rotating mechanism all with the arc plate of upper and lower both sides fixed mounting, the utility model discloses when the particle under the action of pressure and then enter the inside of conveying pipe, through the rotating mechanism to the particle tangential flow filtration operation, simultaneously to the self -cleaning operation of the lipid gel layer of jam, and then prevent the membrane hole from being jammed, make the transmembrane resistance keep stable in long time, make the flux attenuation rate drop, greatly promote the filtration efficiency, powerfully promote the large -scale production process.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical equipment technology, and in particular to a tangential flow filtration device for the preparation of RNA lipid nanoparticles. Background Technology

[0002] RNA lipid nanoparticles are a novel type of nucleic acid delivery carrier. They are formed by the self-assembly of cationic lipids, helper lipids, cholesterol, and polyethylene glycol lipids in a specific ratio to form nanoscale particles. Their core function is to efficiently encapsulate RNA through electrostatic interactions and achieve intracellular delivery by leveraging the fusion properties of the lipid membrane. They are widely used in fields such as vaccine development, gene therapy, and protein replacement therapy.

[0003] In tangential flow filtration of RNA lipid nanoparticles, although tangential flow alleviates the problems of particle accumulation and membrane fouling, the surface of materials such as polyethersulfone commonly used in flat sheet membranes is highly hydrophobic. This hydrophobicity can cause non-specific adsorption of cationic lipids in LNPs through electrostatic and hydrophobic interactions. This adsorption can quickly form a dense lipid gel layer on the membrane surface, clogging the membrane pores and causing a significant increase in transmembrane resistance in a short period of time. This leads to an increase in flux attenuation rate, significantly reducing filtration efficiency and hindering large-scale production. Utility Model Content

[0004] To address the shortcomings of existing technologies, this utility model provides the following technical solution: a tangential flow filtration device for preparing RNA lipid nanoparticles, comprising a conveying tube, a discharge tube fixedly installed on the outer side of the conveying tube near the bottom, and a circular slot symmetrically opened inside the conveying tube, wherein a circular slot is movably engaged with a circular ring block, and an arc-shaped plate is fixedly installed on the inner side of the circular ring block. The delivery tube has a rotating mechanism inside that performs a self-cleaning operation after filtering RNA lipid nanoparticles. The upper and lower ends of the rotating mechanism are fixedly installed with the upper and lower arc plates on the upper and lower sides.

[0005] As an improvement to the above technical solution, the rotating mechanism includes a rotating cylinder, a power fan blade, a stabilizing cylinder, a filter cylinder, a filter membrane, a support plate, a central column, and a spiral scraper blade. The rotating cylinder is fixedly installed at the bottom end of the arc-shaped plate, the power fan blade is fixedly installed on the inner wall of the rotating cylinder, the stabilizing cylinder is fixedly installed on the side of the power fan blade near the center, the filter cylinder is fixedly installed at the bottom end of the rotating cylinder, the filter membrane is fixedly installed on the inner wall of the filter cylinder, the support plate is fixedly installed on the inner wall of the conveying pipe close to the bottom end, the central column is fixedly installed at the top center of the support plate, and the spiral scraper blade is fixedly installed on the outer side of the central column, with the outer side of the spiral scraper blade in contact with the filter membrane.

[0006] As an improvement to the above technical solution, an arc-shaped baffle is fixedly installed on the inner wall of the conveying pipe and on the lower side of the discharge pipe, and the outer side of the arc-shaped baffle is in contact with the outer side of the filter cylinder.

[0007] As an improvement to the above technical solution, the top of the stabilizing cylinder is designed with rounded corners.

[0008] As an improvement to the above technical solution, the top of the support plate is designed to be raised.

[0009] The beneficial effects of this invention are as follows: when the particles enter the conveying pipe under pressure, the rotating mechanism performs tangential flow filtration on the particles and simultaneously cleans the clogged lipid gel layer, thereby preventing the membrane pores from being blocked, keeping the transmembrane resistance stable over a long period of time, reducing the flux decay rate, significantly improving filtration efficiency, and powerfully promoting the large-scale production process. Attached Figure Description

[0010] Figure 1 This is a front view of the filtration device of this utility model; Figure 2 This is a cross-sectional view of the internal structure of this utility model; Figure 3 This utility model Figure 2 Enlarged view of the structure at point A in the middle; Figure 4 This utility model Figure 2 Enlarged view of the structure at point B in the middle.

[0011] Reference numerals: 1. Conveying pipe; 2. Discharge pipe; 3. Circular groove; 31. Circular ring block; 32. Arc plate; 4. Rotating cylinder; 41. Power fan blade; 42. Stabilizing cylinder; 43. Filter cylinder; 44. Filter membrane; 45. Support plate; 46. Central column; 47. Spiral scraper blade. Detailed Implementation

[0012] To make the objectives, technical solutions, and advantages of this utility model clearer, the following provides a more detailed description of the utility model. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of the utility model.

[0013] Reference Appendix Figure 1 ,exist Figure 1 In the diagram, 'a' points to the front view and 'b' points to the right-side view. These views are only used to understand the scheme.

[0014] Please see Figure 1-4This utility model provides a technical solution: a tangential flow filtration device for the preparation of RNA lipid nanoparticles, including a conveying pipe 1, a discharge pipe 2 fixedly installed on the outer side of the conveying pipe 1 near the bottom, and a circular slot 3 symmetrically opened inside the conveying pipe 1. A circular ring block 31 is movably engaged in the circular slot 3, and an arc plate 32 is fixedly installed on the inner side of the circular ring block 31. The inside of the delivery tube 1 is equipped with a rotating mechanism that performs a self-cleaning operation after filtering RNA lipid nanoparticles in a circular rotation. Both the upper and lower ends of the rotating mechanism are fixedly installed with the upper and lower arc plates 32 on the upper and lower sides.

[0015] In this embodiment, when the particles enter the delivery pipe 1 under pressure, the rotating mechanism performs tangential flow filtration of the particles and self-cleans the blocked lipid gel layer, thereby preventing the membrane pores from being blocked, keeping the transmembrane resistance stable over a long period of time, reducing the flux decay rate, significantly improving filtration efficiency, and powerfully promoting the large-scale production process.

[0016] Specifically, the rotating mechanism includes a rotating cylinder 4, a power fan blade 41, a stabilizing cylinder 42, a filter cylinder 43, a filter membrane 44, a support plate 45, a central column 46, and a spiral scraper blade 47. The rotating cylinder 4 is fixedly installed at the bottom end of the arc-shaped plate 32. The power fan blade 41 is fixedly installed on the inner wall of the rotating cylinder 4. The stabilizing cylinder 42 is fixedly installed on the side of the power fan blade 41 near the center. The filter cylinder 43 is fixedly installed at the bottom end of the rotating cylinder 4. The filter membrane 44 is fixedly installed on the inner wall of the filter cylinder 43. The support plate 45 is fixedly installed on the inner wall of the conveying pipe 1 close to the bottom end. The central column 46 is fixedly installed at the top center of the support plate 45. The spiral scraper blade 47 is fixedly installed on the outer side of the central column 46, and the outer side of the spiral scraper blade 47 is in contact with the filter membrane 44.

[0017] In this embodiment, the internal structure of the rotating mechanism filters the particles while simultaneously cleaning the clogged lipid gel layer, thereby preventing the membrane pores from becoming blocked.

[0018] Specifically, an arc-shaped baffle is fixedly installed on the inner wall of the conveying pipe 1 and below the discharge pipe 2, and the outer side of the arc-shaped baffle is in contact with the outer side of the filter cylinder 43.

[0019] In this embodiment, small molecule substances can be discharged through the discharge pipe 2 under the restriction of the arc-shaped baffle, preventing accumulation.

[0020] Specifically, the top of the stable cylinder 42 has a rounded corner design.

[0021] In this embodiment, particles are prevented from agglomerating at the top of the stabilizing cylinder 42.

[0022] Specifically, the top of the support plate 45 has a raised design.

[0023] In this embodiment, particles are prevented from accumulating on the top of the support plate 45.

[0024] In use, the upper and lower ends of the conveying pipe 1 are connected to corresponding pipelines, and the conveying pipe 1 is placed vertically. The discharge pipe 2 is connected to the corresponding pipeline. When the particles enter the conveying pipe 1 from the upper end under pressure, the particles will enter the rotating cylinder 4 and come into contact with the power fan blade 41. The particles have an impact force on the power fan blade 41, causing the particles to drive the power fan blade 41 to rotate. The power fan blade 41 drives the stabilizing cylinder 42 and the rotating cylinder 4 to rotate synchronously. The rotating cylinder 4 drives the circular locking block 31 to rotate along the circular locking groove 3 through the arc plate 32. The rotating cylinder 4 drives the filter cylinder 43 to rotate, and the filter cylinder 43 drives the filter membrane 44 to rotate. When the particles enter the filter cylinder 43 downwards, they will hit the spiral scraper blade 47 and move along the spiral of the spiral scraper blade 47. As the particles move downwards and away from the center, the filter membrane 44 filters them, allowing small molecules to be filtered to the outside of the filter membrane 44 and pass through the filter cylinder 43 to the inner wall between the filter cylinder 43 and the conveying pipe 1. At this time, the small molecules move downwards due to pressure and are discharged through the discharge pipe 2 under the restriction of the arc-shaped baffle. At this time, the cationic lipids in the particles will undergo non-specific adsorption with the filter membrane 44, thereby forming a lipid gel layer that blocks the membrane pores. When the filter membrane 44 rotates, the lipid gel layer is scraped and cleaned by the fixed spiral scraper blade 47, which facilitates subsequent filtration of particles and prevents the membrane pores from being blocked. This keeps the transmembrane resistance stable over a long period of time, reduces the flux decay rate, greatly improves the filtration efficiency, and powerfully promotes the large-scale production process.

[0025] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.

Claims

1. A tangential flow filtration device for preparing RNA lipid nanoparticles, comprising a delivery tube (1), characterized in that: A discharge pipe (2) is fixedly installed on the outside of the conveying pipe (1) near the bottom. A circular slot (3) is symmetrically opened inside the conveying pipe (1). A circular slot (3) is movably engaged with a circular ring block (31). An arc plate (32) is fixedly installed on the inner side of the circular ring block (31). The delivery tube (1) is equipped with a rotating mechanism inside in a circular rotation to perform self-cleaning operation after filtering RNA lipid nanoparticles. The upper and lower ends of the rotating mechanism are fixedly installed with the arc plates (32) on the upper and lower sides.

2. The tangential flow filtration device for preparing RNA lipid nanoparticles according to claim 1, characterized in that: The rotating mechanism includes a rotating cylinder (4), a power fan blade (41), a stabilizing cylinder (42), a filter cylinder (43), a filter membrane (44), a support plate (45), a central column (46), and a spiral scraper (47). The rotating cylinder (4) is fixedly installed at the bottom end of the arc plate (32). The power fan blade (41) is fixedly installed on the inner wall of the rotating cylinder (4). The stabilizing cylinder (42) is fixedly installed on the side of the power fan blade (41) near the center. The filter cylinder (43) is fixedly installed at the bottom end of the rotating cylinder (4). The filter membrane (44) is fixedly installed on the inner wall of the filter cylinder (43). The support plate (45) is fixedly installed on the inner wall of the conveying pipe (1) close to the bottom end. The central column (46) is fixedly installed at the top center of the support plate (45). The spiral scraper (47) is fixedly installed on the outer side of the central column (46). The outer side of the spiral scraper (47) is in contact with the filter membrane (44).

3. The tangential flow filtration device for preparing RNA lipid nanoparticles according to claim 2, characterized in that: An arc-shaped baffle is fixedly installed on the inner wall of the conveying pipe (1) and on the lower side of the discharge pipe (2), and the outer side of the arc-shaped baffle is in contact with the outer side of the filter cylinder (43).

4. The tangential flow filtration device for preparing RNA lipid nanoparticles according to claim 2, characterized in that: The top of the stabilizing cylinder (42) is designed with rounded corners.

5. The tangential flow filtration device for preparing RNA lipid nanoparticles according to claim 2, characterized in that: The top of the support plate (45) is designed to be raised.

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