A multi-layer filtering device for biochemical reagent purification

Abstract

The utility model discloses a multilayer filtering device for biochemical reagent purification, including jar body and sealing cover, be equipped with liquid inlet on the sealing cover, and jar body side is equipped with liquid discharge port, rotatablely be equipped with support frame in the jar body, and the detachable filter assembly is equipped on the support frame, the filter assembly includes mounting panel, and the coaxial first filter drum, second filter drum and third filter drum are equipped on the mounting panel, and the mounting block is rotatablely equipped on the mounting panel, and the first connecting rod is clamped and is connected with the sealing cover on the mounting block another end, and the first connecting rod both sides are equipped with first scraper, second scraper and third scraper, and the first scraper is butt joint with first filter drum inner wall, and the second scraper is butt joint with second filter drum inner wall, and the third scraper is butt joint with third filter drum inner wall. Through mounting panel drive first filter drum, second filter drum and third filter drum rotate, can improve the filtering speed under the centrifugal action, and first scraper, second scraper, third scraper avoid filter hole to be blocked and influence the filtering effect.

Description

Technical Field

[0001] This utility model belongs to the field of biochemical reagent purification technology, and in particular relates to a multi-layer filtration device for biochemical reagent purification. Background Technology

[0002] Biochemical reagents refer to biological materials or organic compounds used in life science research and clinical diagnosis, including biological extracts or chemically synthesized compounds, covering categories such as carbohydrates, enzymes, nucleic acids, and amino acids. In the production of biochemical reagents such as proteins, the extract is filtered sequentially using filtration devices based on different molecular sizes, thus requiring multiple filtration layers. However, current technologies generally rely on gravity to pass the solution through the filtration device, resulting in low filtration efficiency. Furthermore, the filter plates lack corresponding cleaning components, and when the surface of the filtration device becomes covered with protein molecules of varying sizes, it affects the filtration effect, making the filtration process less effective. Utility Model Content

[0003] Based on the above background, the purpose of this utility model is to provide a multi-layer filtration device for the purification of biochemical reagents.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: A multi-layer filtration device for purifying biochemical reagents includes a tank and a sealing cap located on top of the tank. The sealing cap is provided with a liquid inlet, and the side of the tank is provided with a liquid outlet; The tank is equipped with a rotatable support frame, and a filter assembly is detachably mounted on the support frame; The filter assembly includes a mounting plate, on which a first filter cylinder, a second filter cylinder, and a third filter cylinder are coaxially arranged, with the first filter cylinder disposed inside the second filter cylinder and the second filter cylinder disposed inside the third filter cylinder. The first filter cartridge contains a first filter chamber, the space between the first and second filter cartridges forms a second filter chamber, and the space between the second and third filter cartridges forms a third filter chamber. The mounting plate is rotatably provided with a mounting block, and a first connecting rod is snapped onto the mounting block. The other end of the first connecting rod is snapped into the sealing cover. The first connecting rod is provided with a first scraper, a second scraper and a third scraper on both sides. The first scraper abuts against the inner wall of the first filter cylinder, the second scraper abuts against the inner wall of the second filter cylinder, and the third scraper abuts against the inner wall of the third filter cylinder.

[0005] Furthermore, a motor is provided at the bottom of the tank, and the output end of the motor extends into the tank and is connected to the support frame; The support frame is a cross shape; The mounting plate has a mounting groove at its bottom, which is cross-shaped, and the support frame is snapped into the mounting groove.

[0006] Furthermore, a first support ring is rotatably provided on the inner wall of the tank via a bearing, and the side of the support frame is connected to the inner wall of the first support ring.

[0007] Furthermore, a second support ring is provided on the upper end of the inner wall of the tank via a bearing, and a support plate is provided on the outer side of the third filter cylinder. The support plate is detachably mounted on the top of the second support ring.

[0008] Furthermore, the second support ring is provided with a positioning hole, and the bottom of the support plate is provided with a corresponding positioning block, which is inserted into the positioning hole.

[0009] Furthermore, the tops of the two second scrapers are connected to second connecting rods, and the second connecting rods are provided with a first square groove in the middle, into which the first connecting rod is inserted; The tops of the two third scrapers are connected to a third connecting rod, and the third connecting rod has a second square groove in the middle, into which the first connecting rod is inserted; The third connecting rod is located at the top of the second connecting rod.

[0010] Furthermore, the bottom of the sealing cover is provided with a first slot, and second slots are provided on both sides of the first slot; One end of the first connecting rod is engaged with the first slot, and the second and third connecting rods are engaged with the second slot.

[0011] Furthermore, the bottom of the first connecting rod is disposed on the first base plate, the first base plate is inserted into the bottom of the first filter chamber, and the two first scraper rods are symmetrically disposed on the top of the first base plate; A second base plate is inserted into the bottom of the second filter chamber, and two second scrapers are symmetrically arranged on the top of the second base plate; A third base plate is inserted into the bottom of the third filter chamber, and two third scrapers are symmetrically arranged on the top of the third base plate.

[0012] Furthermore, the tank body is provided with a vertical rod on its side, and the sealing cover is slidably connected to the vertical rod via a sliding frame; The upright is equipped with a cylinder, and the output end of the cylinder is connected to the sliding frame.

[0013] This utility model has the following beneficial effects: 1. The motor drives the support frame to rotate, which in turn drives the first, second, and third filter cartridges to rotate via the mounting plate. Under centrifugal force, the filtration speed can be increased. Large protein molecules adhere to the inner wall of the first filter cartridge, medium protein molecules adhere to the inner wall of the second filter cartridge, and small protein molecules adhere to the inner wall of the third filter cartridge. The filtered waste liquid enters the tank through the side of the third filter cartridge and is discharged from the drain port. During the rotation of the filter assembly, the first, second, and third scrapers scrape the inner walls of the first, second, and third filter cartridges respectively to prevent the filter holes from being blocked and affecting the filtration effect.

[0014] 2. The first connecting rod is square and is connected to the second and third connecting rods through the first and second square grooves. The top of the first connecting rod is engaged with the sealing cover to prevent the first connecting rod from rotating with the filter assembly, thus better ensuring the scraping effect of the first, second, and third scrapers. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a front structural diagram of the present invention; Figure 3 For the present utility model Figure 2 Schematic diagram of the AA cross-section structure; Figure 4 This is a three-dimensional structural diagram of the first connecting rod, the second connecting rod, and the third connecting rod of this utility model; Figure 5 This is a three-dimensional structural diagram of the sealing cap of this utility model; Figure 6 This is a three-dimensional structural diagram of the filter assembly of this utility model.

[0017] Of which: 1. Tank body; 11. Drain outlet; 2. Sealing cap; 21. Liquid inlet; 22. First slot; 23. Second slot; 24. Upright rod; 25. Cylinder; 26. Sliding frame; 3. Support frame; 31. Motor; 32. First support ring; 33. Second support ring; 34. Positioning hole; 4. Filter assembly; 41. First filter cartridge; 42. Second filter cartridge; 43. Third filter cartridge; 44. Mounting plate; 45. Mounting block; 46. Mounting groove; 47. Support plate; 48. Positioning block; 5. First connecting rod; 51. First scraper rod; 52. Second scraper rod; 53. Third scraper rod; 54. Second connecting rod; 55. First square groove; 56. Third connecting rod; 57. Second square groove; 58. First base plate; 59. Second base plate; 510. Third base plate. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0020] Furthermore, in this utility model, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. When the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed by this utility model.

[0021] like Figure 1-6As shown, a multi-layer filtration device for purifying biochemical reagents includes a tank 1 and a sealing cap 2 located on the top of the tank 1. The sealing cap 2 has an inlet 21, and the tank 1 has a drain port 11 located on its side at the lower end. A motor 31 is located at the bottom of the tank 1, with its output end extending into the tank 1 and connected to a support frame 3. The support frame 3 is rotatably mounted inside the tank 1, and a detachable filter assembly 4 is mounted on the support frame 3. The filter assembly 4 includes a mounting plate 44, on which a first filter cylinder 41, a second filter cylinder 42, and a third filter cylinder 43 are coaxially mounted. One end of the inlet 21 extends into the first filter cylinder 41 and is located near the middle of the first filter cylinder 41 in its longitudinal direction. The first filter cylinder 41 is located inside the second filter cylinder 42, which has a... The third filter cylinder 43 is inside the first filter cylinder 41, which is the first filter chamber. The second filter chamber is located between the first filter cylinder 41 and the second filter cylinder 42. The third filter chamber is located between the second filter cylinder 42 and the third filter cylinder 43. The filter hole diameters on the first filter cylinder 41, the second filter cylinder 42, and the third filter cylinder 43 decrease sequentially. A mounting block 45 is rotatably provided on the mounting plate 44. The mounting block 45 is located inside the first filter cylinder 41. A first connecting rod 5 is snapped onto the mounting block 45. The other end of the first connecting rod 5 is snapped onto the sealing cover 2. A first scraper 51, a second scraper 52, and a third scraper 53 are provided on both sides of the first connecting rod 5. The first scraper 51 abuts against the inner wall of the first filter cylinder 41, the second scraper 52 abuts against the inner wall of the second filter cylinder 42, and the third scraper 53 abuts against the inner wall of the third filter cylinder 43. The dissolved purified solution enters the filter assembly 4 through the inlet 21. The motor 31 drives the support frame 3 to rotate, which in turn drives the first filter cylinder 41, the second filter cylinder 42, and the third filter cylinder 43 to rotate through the mounting plate 44. Under centrifugal action, the filtration speed can be increased. Large protein molecules adhere to the inner wall of the first filter cylinder 41, medium protein molecules adhere to the inner wall of the second filter cylinder, and small protein molecules adhere to the inner wall of the third filter cylinder 43. The filtered waste liquid enters the tank 1 through the side of the third filter cylinder 43 and is discharged from the drain port 11. During the rotation of the filter assembly 4, the first scraper, the second scraper, and the third scraper scrape the inner walls of the first filter cylinder 41, the second filter cylinder 42, and the third filter cylinder 43, respectively, to prevent the filter holes from being blocked and affecting the filtration effect.

[0022] The support frame 3 is cross-shaped; the mounting plate 44 has a mounting groove 46 at its bottom, which is cross-shaped. The support frame 3 is engaged in the mounting groove 46. A first support ring 32 is rotatably mounted on the inner wall of the tank 1 via a bearing. The first support ring 32 is located at the lower end of the inner wall of the tank 1. The side of the support frame 3 is connected to the inner wall of the first support ring 32. A second support ring 33 is rotatably mounted on the upper end of the inner wall of the tank 1 via a bearing. A support plate 47 is provided on the outer side of the third filter cartridge 43. The support plate 47 is detachably mounted on the top of the second support ring 33. The second support ring 33 has a positioning hole 34. A positioning block 48 is correspondingly provided at the bottom of the support plate 47 and is inserted into the positioning hole 34. The mounting plate 44 is engaged with the support frame 3, and the second support ring 33 provides support for the upper end of the filter assembly 4, thereby improving the stability of the rotation of the filter assembly 4.

[0023] Among them, the tops of the two second scraper rods 52 are connected to the second connecting rods 54, and the second connecting rods 54 have a first square groove 55 in the middle, into which the first connecting rod 5 is inserted; the tops of the two third scraper rods 53 are connected to the third connecting rods 56, and the third connecting rods 56 have a second square groove 57 in the middle, into which the first connecting rod 5 is inserted; the third connecting rod 56 is located at the top of the second connecting rods 54, and the bottom of the sealing cover 2 has a first retaining groove 22, with second retaining grooves 23 on both sides of the first retaining groove 22; the first connecting rod 53 is connected to the second connecting rods 54. One end of the connecting rod 5 is engaged with the first slot 22, and the second connecting rod 54 and the third connecting rod 56 are engaged with the second slot 23. The bottom of the first connecting rod 5 is located on the first base plate 58, which is inserted into the bottom of the first filter chamber, and two first scraper rods 51 are symmetrically located on the top of the first base plate 58. The bottom of the second filter chamber is connected to the second base plate 59, and two second scraper blades are symmetrically located on the top of the second base plate 59. The bottom of the third filter chamber is connected to the third base plate 510, and two third scraper blades are symmetrically located on the top of the third base plate 510. The first connecting rod 5 is square and is connected to the second connecting rod 54 and the third connecting rod 56 through the first square groove 55 and the second square groove 57. The top of the first connecting rod 5 is engaged with the sealing cover 2 to prevent the first connecting rod 5 from rotating with the filter assembly 4, thus better ensuring the scraping effect of the first scraper 51, the second scraper 52 and the third scraper 53. Furthermore, the arrangement of the first bottom plate 58, the second bottom plate 59 and the third bottom plate 510 facilitates the sequential removal of protein molecules from the third filter cylinder 43, the second filter cylinder 42 and the first filter cylinder 41 after filtration.

[0024] The tank body 1 has a vertical rod 24 on its side, and the sealing cover 2 is slidably connected to the vertical rod 24 via a sliding frame 26. The vertical rod 24 is equipped with a cylinder 25, and the output end of the cylinder 25 is connected to the sliding frame 26. The cylinder 25 drives the sliding frame 26 to connect, thereby controlling the sealing cover 2 to open the top of the tank body 1 or close the top of the cover.

[0025] The working principle of this utility model is as follows: Before starting work, the filter assembly 4 is installed with the tank 1. First, the first connecting rod 5 is inserted into the mounting block 45. The first scraper 51 and the first base plate 58 are located in the first filter chamber. The second connecting rod 54 is inserted into the first connecting rod 5. The second scraper 52 and the second base plate 59 are located in the second filter chamber. The third connecting rod 56 is inserted into the first connecting rod 5 and is located on top of the second connecting rod 54. The third scraper 53 and the third base plate 510 are located in the third filter chamber. Then, the mounting groove 46 of the mounting plate 44 is engaged with the support frame 3. The positioning block 48 is inserted into the positioning hole 34, completing the installation of the filter assembly 4. The sealing cover 2 is closed on the top of the tank 1. The first slot 22 and the second slot 23 are respectively engaged with the first connecting rod 5 and the third connecting rod 56, the second connecting rod 57 and the third connecting rod 58. The rod 54 is engaged, and the dissolved purified solution enters the filter assembly 4 through the inlet 21. The motor 31 drives the support frame 3 to rotate, which in turn drives the first filter cylinder 41, the second filter cylinder 42, and the third filter cylinder 43 to rotate through the mounting plate 44. Under centrifugal action, the filtration speed can be increased. Large protein molecules adhere to the inner wall of the first filter cylinder 41, medium protein molecules adhere to the inner wall of the second filter cylinder, and small protein molecules adhere to the inner wall of the third filter cylinder 43. The filtered waste liquid enters the tank 1 through the side of the third filter cylinder 43 and is discharged from the drain port 11. During the rotation of the filter assembly 4, the first scraper, the second scraper, and the third scraper scrape the inner walls of the first filter cylinder 41, the second filter cylinder 42, and the third filter cylinder 43 respectively to prevent the filter holes from being blocked and affecting the filtration effect.

[0026] Of course, the above description is not intended to limit the present utility model, and the present utility model is not limited to the examples given above. Any changes, modifications, additions or substitutions made by those skilled in the art within the scope of the present utility model should also fall within the protection scope of the present utility model.

Claims

1. A multi-layer filtration device for purifying biochemical reagents, comprising a tank (1) and a sealing cap (2) disposed on the top of the tank (1), characterized in that: The sealing cap (2) is provided with a liquid inlet (21), and the tank body (1) is provided with a liquid outlet (11) on the side. The tank (1) is rotatably provided with a support frame (3), and a filter assembly (4) is detachably provided on the support frame (3). The filter assembly (4) includes a mounting plate (44), on which a first filter cylinder (41), a second filter cylinder (42) and a third filter cylinder (43) are coaxially arranged, and the first filter cylinder (41) is disposed inside the second filter cylinder (42), and the second filter cylinder (42) is disposed inside the third filter cylinder (43); The first filter cylinder (41) contains a first filter chamber, the first filter cylinder (41) and the second filter cylinder (42) are located in a second filter chamber, and the second filter cylinder (42) and the third filter cylinder (43) are located in a third filter chamber. The mounting plate (44) is rotatably provided with a mounting block (45), and a first connecting rod (5) is snapped onto the mounting block (45). The other end of the first connecting rod (5) is snapped onto the sealing cover (2). The first connecting rod (5) is provided with a first scraper (51), a second scraper (52) and a third scraper (53) on both sides. The first scraper (51) abuts against the inner wall of the first filter cylinder (41), the second scraper (52) abuts against the inner wall of the second filter cylinder (42), and the third scraper (53) abuts against the inner wall of the third filter cylinder (43).

2. The multi-layer filtration device for purifying biochemical reagents according to claim 1, characterized in that: The bottom of the tank (1) is provided with a motor (31), the output end of which extends into the tank (1) and is connected to the support frame (3); The support frame (3) is a cross; The mounting plate (44) has a mounting groove (46) at the bottom. The mounting groove (46) is cross-shaped, and the support frame (3) is snapped into the mounting groove (46).

3. The multi-layer filtration device for purifying biochemical reagents according to claim 2, characterized in that: The inner wall of the tank (1) is provided with a first support ring (32) which rotates through a bearing, and the side of the support frame (3) is connected to the inner wall of the first support ring (32).

4. The multi-layer filtration device for purifying biochemical reagents according to claim 3, characterized in that: The upper end of the inner wall of the tank (1) is provided with a second support ring (33) that rotates through a bearing, and the outer side of the third filter cylinder (43) is provided with a support plate (47), which is detachably located on the top of the second support ring (33).

5. The multi-layer filtration device for purifying biochemical reagents according to claim 4, characterized in that: The second support ring (33) is provided with a positioning hole (34), and the bottom of the support plate (47) is provided with a positioning block (48), which is inserted into the positioning hole (34).

6. The multi-layer filtration device for purifying biochemical reagents according to claim 1, characterized in that: The top of the two second scraper rods (52) is connected to a second connecting rod (54), and the second connecting rod (54) has a first square groove (55) in the middle, and the first connecting rod (5) is inserted into the first square groove (55); The top of the two third scraper rods (53) are connected to a third connecting rod (56), and the third connecting rod (56) is provided with a second square groove (57) in the middle, and the first connecting rod (5) is inserted into the second square groove (57); The third connecting rod (56) is located on top of the second connecting rod (54).

7. The multi-layer filtration device for purifying biochemical reagents according to claim 6, characterized in that: The sealing cover (2) has a first slot (22) at the bottom, and a second slot (23) is provided on both sides of the first slot (22); One end of the first connecting rod (5) is engaged with the first slot (22), and the second connecting rod (54) and the third connecting rod (56) are engaged with the second slot (23).

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