A microelectrode puller

Abstract

The utility model discloses a kind of microelectrode drawing instruments, belong to electrophysiology, microinjection and micro-operation technical field, including vertical guide rail, first clamping component, second clamping component, heating component and third limiting structure, vertical guide rail is provided with first limiting structure and second limiting structure;First clamping component is slidably arranged on vertical guide rail, located above first limiting structure;Second clamping component is slidably arranged on vertical guide rail, between first limiting structure and second limiting structure;Heating component is slidably arranged on vertical guide rail, between first clamping component and second clamping component, heating component moves along with second clamping component;Third limiting structure is below heating component.The utility model is driven heating component synchronous downward movement under the action of gravity by second clamping component, keep heating area dynamic matching deformation part, guarantee heating even, temperature control accurate, improve the preparation precision of microelectrode taper.

Description

Technical Field

[0001] This utility model relates to the fields of electrophysiology, microinjection and micromanipulation, and in particular to a microelectrode pulling device. Background Technology

[0002] In patch-clamp technology, glass microelectrodes are key components for achieving high-resistivity sealing and accurately measuring intracellular electrical signals. The precision of their tip diameter (typically less than 1 micrometer), geometry, taper, and symmetry directly determines the success rate of sealing and signal quality. Currently, microelectrodes are mainly fabricated by heating and stretching glass tubes using a stretching apparatus, with two main stretching methods: horizontal and vertical.

[0003] Existing horizontal drawing instruments typically use metal wires or platinum sheets to heat a glass tube until it melts, then draw it bidirectionally using gravity or electromagnetic force, and finally cool and solidify it. However, during heating, the molten glass is prone to sagging due to gravity, resulting in geometric asymmetry between the two electrode tips and making it difficult to consistently obtain qualified products with flat tips and consistent taper.

[0004] While vertical drawing instruments can alleviate the symmetry problem caused by gravity, the deformation area of ​​the glass tube will shift during the drawing process. Current technology lacks an effective means to dynamically track and match this shifting deformation area with the heating zone, resulting in uneven heating and inaccurate temperature control. Consequently, the fabricated microelectrodes have unsatisfactory taper and irregular tip shape, failing to meet the requirements of high-precision applications.

[0005] Therefore, regardless of whether the drawing method is horizontal or vertical, the existing equipment has obvious limitations in the core process, making it difficult to stably and in batches produce glass microelectrodes that meet the extremely high requirements of the patch clamp system, thus restricting the progress of related scientific research and experiments. Utility Model Content

[0006] The purpose of this invention is to provide a microelectrode pulling device to solve the problems existing in the prior art. Under the action of gravity, the heating component moves downward synchronously by relying on the second clamping component, keeping the heating area dynamically matched with the deformation part, ensuring uniform heating and accurate temperature control, and improving the preparation accuracy of microelectrode taper.

[0007] To achieve the above objectives, this utility model provides the following solution: This utility model provides a microelectrode pulling device, including a vertical guide rail, a first clamping assembly, a second clamping assembly, a heating assembly, and a third limiting structure. The vertical guide rail is provided with the first limiting structure and the second limiting structure. The first clamping assembly is slidably disposed on the vertical guide rail, located above the first limiting structure. The second clamping assembly is slidably disposed on the vertical guide rail, located between the first limiting structure and the second limiting structure. The heating assembly is slidably disposed on the vertical guide rail, located between the first clamping assembly and the second clamping assembly, and moves with the second clamping assembly under the action of gravity. The third limiting structure is located below the heating assembly and is used to limit the lower position of the heating assembly.

[0008] In one embodiment, the third limiting structure includes a limiting seat and a micro switch. The micro switch is disposed on the limiting seat and is used to acquire the position information of the heating component. The limiting seat is used to limit the low position of the heating component.

[0009] In one embodiment, a fourth limiting structure is further included, which is located between the second clamping component and the second limiting structure, and has a retractable limiting portion for limiting the stop position of the second clamping component.

[0010] In one embodiment, the fourth limiting structure includes an electromagnetic structure and a locking tongue, the electromagnetic structure being used to control the extension and retraction of the locking tongue, and the locking tongue being used to define the stop position of the second clamping assembly.

[0011] In one embodiment, the device further includes a first check component and a second check component located above the first limiting structure. The check position of the second check component is higher than that of the first check component. The first check component is used to restrict the first clamping component from moving upward, and the second check component is used to restrict the first clamping component from moving downward.

[0012] In one embodiment, the first clamping assembly includes a first slider, a first locking block, and a first clamping plate. The first clamping plate is connected to the first slider, the first slider is slidably disposed on the vertical guide rail, the first clamping plate is provided with a first V-shaped groove for fixing the glass tube, and the first locking block is alternately connected to both sides of the first V-shaped groove.

[0013] In one embodiment, the second clamping assembly includes a second slider, a second locking block, and a second clamping plate. The second clamping plate is connected to the second slider, the second slider is slidably disposed on the vertical guide rail, the second clamping plate is provided with a second V-shaped groove for fixing the glass tube, and the second locking block is alternately connected to both sides of the second V-shaped groove.

[0014] In one embodiment, the device further includes a first fine-tuning structure and a second fine-tuning structure. The first clamping plate is connected to the first slider via the first fine-tuning structure, and the bottom of the first clamping plate is used to abut against the heating component. The second clamping plate is connected to the second slider via the second fine-tuning structure, and the top of the second clamping plate is used to abut against the heating component.

[0015] In one embodiment, a limit switch is also included, which is disposed near the second limiting structure for detecting a signal that the second clamping assembly has descended to the second limiting structure.

[0016] In one embodiment, a counterweight block is further included, the counterweight block comprising a plurality of counterweight plates, the counterweight plates being detachably connected to the bottom of the second clamping plate by bolts.

[0017] The present invention achieves the following technical advantages over the prior art: Both the second clamping component and the heating component of this invention are slidably mounted on a vertical guide rail. Under the action of gravity, the second clamping component can drive the heating component to move downward synchronously, and the third limiting structure limits the low position of the heating component. This can maintain the dynamic matching of the heating area with the deformation part, ensure uniform heating and accurate temperature control, and improve the preparation accuracy of the microelectrode taper. At the same time, the first clamping component is slidably mounted on the vertical guide rail, which is convenient to move upward after the drawing process is completed, so as to avoid the influence of the heating component and smoothly remove the drawn microelectrode. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of the microelectrode pulling device in this embodiment of the present invention; Figure 2 This is a schematic diagram of the microelectrode pulling device removing the protective cover in an embodiment of this utility model; Figure 3 for Figure 2 Front view of the structure shown; Figure 4 for Figure 2 Side view of the structure shown; Figure 5 This is a schematic diagram of the internal structure of the preparation module in an embodiment of this utility model; Figure 6 for Figure 5 Side view of the structure shown; Figure 7 for Figure 5 The main view of the structure shown; Figure 8 This is a schematic diagram of the control principle in an embodiment of this utility model; The components include: 1. Vertical guide rail; 2. First clamping assembly; 3. Heating assembly; 4. Second clamping assembly; 5. Third limiting structure; 6. Fourth limiting structure; 8. Limit switch; 9. Counterweight; 10. Preparation module; 20. Control module; 30. Support base; 40. Glass tube. 11. First limiting structure; 12. Second limiting structure; 13. Top limiting structure; 21. First slider; 22. First clamping plate; 23. First locking block; 24. First fine-tuning structure; 31. Ceramic base; 32. Heating wire; 41. Second slider; 42. Second clamping plate; 43. Second locking block; 44. Second fine-tuning structure; 51. Limit seat; 52. Micro switch; 61. Electromagnetic structure; 62. Locking tongue; 71. First check valve assembly; 72. Second check valve assembly; 101. Shell; 102. Protective cover; 103. Cover plate. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] The purpose of this invention is to provide a microelectrode pulling device to solve the problems existing in the prior art. Under the action of gravity, the heating component is driven to move downward synchronously by the second clamping component, so as to keep the heating area dynamically matched with the deformation part, ensure uniform heating and accurate temperature control, and improve the preparation accuracy of microelectrode taper.

[0022] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] like Figures 1-8As shown, this utility model provides a microelectrode pulling device, including a preparation module 10 and a control module 20. The preparation module 10 and the control module 20 can be integrated into one unit or separately installed on a support base 30. The preparation module 10 includes a vertical guide rail 1, a first clamping component 2, a second clamping component 4, a heating component 3, and a third limiting structure 5. The vertical guide rail 1 is provided with a first limiting structure 11 and a second limiting structure 12. Both the first limiting structure 11 and the second limiting structure 12 can be limiting blocks, which are installed on the vertical guide rail 1 to limit the position of the first clamping component 2 and the second clamping component 4. The first clamping component 2 is slidably disposed on the vertical guide rail 1, located above the first limiting structure 11. The first limiting structure 11 can limit the lowest position of the first clamping component 2, that is, the first clamping component 2 can clamp the upper end of the glass tube 40 and fix the glass tube 40. The second clamping assembly 4 is slidably disposed on the vertical guide rail 1, located between the first limiting structure 11 and the second limiting structure 12. The second limiting structure 12 restricts the lowest position of the second clamping assembly 4. After the second clamping assembly 4 clamps the lower end of the glass tube 40, the glass tube 40 can be pulled by the downward movement of the second clamping assembly 4 relative to the first clamping assembly 2. The heating assembly 3 is slidably disposed on the vertical guide rail 1, located between the first clamping assembly 2 and the second clamping assembly 4. The heating assembly 3 can heat the glass tube 40 to melt it before pulling. The heating assembly 3 moves with the second clamping assembly 4 under gravity, adapting to changes and movements in the deformed parts during the pulling process. The third limiting structure 5 is located below the heating assembly 3, used to limit the lower position of the heating assembly 3. The distance the heating assembly 3 moves is set as needed. After the heating assembly 3 moves to the position of the third limiting structure 5, it is restricted from further downward movement. At this time, the second clamping assembly 4 continues to move downward, breaking the glass tube 40 and completing the pulling process.

[0024] The second clamping component 4 and the heating component 3 of this utility model are both slidably disposed on the vertical guide rail 1. Under the action of gravity, the second clamping component 4 can drive the heating component 3 to move downward synchronously, and the third limiting structure 5 can limit the low position of the heating component 3, which can maintain the dynamic matching of the heating area with the deformation part, ensure uniform heating and accurate temperature control, and improve the preparation accuracy of the microelectrode taper. At the same time, the first clamping component 2 is slidably disposed on the vertical guide rail 1, which is convenient to move upward after the drawing process is completed, so as to avoid the influence of the heating component 3 (the heating component 3 includes an annular heating wire 32, and the glass tube 40 is heated on the inner diameter side of the heating wire 32. The point where the glass tube 40 is broken is usually located on the inner diameter side of the heating wire 32. Therefore, if the glass tube 40 is moved radially directly after being broken, it will be affected by the heating wire 32, resulting in damage to the drawn glass tube 40) and the drawn microelectrode (glass tube 40) can be successfully removed.

[0025] In one implementation, such as Figure 5 and Figure 7 As shown, the heating assembly 3 also includes a ceramic base 31, and a heating wire 32 is mounted on the ceramic base 31. The ceramic base 31 has good heat insulation and electrical insulation properties, which can effectively ensure the heating efficiency of the heating wire 32.

[0026] In one implementation, such as Figures 1-4 As shown, the vertical guide rail 1 is mounted on the housing 101, which provides vertical support for the guide rail 1. Other limiting structures or auxiliary structures, besides those mounted on the vertical guide rail 1, can also be mounted on the housing 101. A cover plate 103 is provided on the top of the housing 101 to cover the top of the housing 101 and keep the internal environment of the housing 101 clean. A protective cover 102 is also included, which is hinged to the housing 101. The protective cover 102 is used to cover the main area where the glass tube 40 is installed and drawn, thereby improving the safety and cleanliness of the drawing process.

[0027] In one implementation, such as Figures 5-7 As shown, the third limiting structure 5 includes a limiting seat 51 and a micro switch 52. The micro switch 52 is disposed on the limiting seat 51 and is used to obtain the position information of the heating component 3 and transmit the signal to the control module 20 so as to control the heating component 3 to stop heating (during one-step pulling) or delay heating (during two-step pulling) according to the corresponding signal. The limiting seat 51 is used to limit the low position of the heating component 3, that is, the heating component 3 is limited to the lowest position after falling by the limiting seat 51.

[0028] In one implementation, such as Figures 2-7 As shown, it also includes a fourth limiting structure 6, which is located between the second clamping assembly 4 and the second limiting structure 12. The fourth limiting structure 6 has a retractable limiting part for limiting the stop position of the second clamping assembly 4. The fourth limiting structure 6 is used in the two-step pulling process. In the first step of pulling, the second clamping assembly 4 falls onto the retractable limiting part for limiting. In the second step of pulling, after the retractable limiting part retracts, the second clamping assembly 4 continues to move downward.

[0029] In one implementation, such as Figures 2-7 As shown, the fourth limiting structure 6 includes an electromagnetic structure 61 and a locking tongue 62. The electromagnetic structure 61 controls the extension and retraction of the locking tongue 62, which, as a retractable limiting part, limits the stop position of the second clamping assembly 4. The electromagnetic structure 61 can be controlled by the control module 20 to realize the pulling process according to the set program.

[0030] During the preparation process, the glass tube 40 is first installed on the first clamping assembly 2. Then, the locking tongue 62 of the fourth limiting structure 6 is controlled to retract, and the second clamping assembly 4 is manually pushed upward. Due to the vertical characteristics, as the second clamping assembly 4 moves upward, the second V-groove aligns with the first V-groove, automatically placing the glass tube 40 into a vertical position until the second clamping assembly 4 presses against the ceramic base 31 of the heating assembly 3. The ceramic base 31 presses against the second clamping assembly 4, so that the glass tube 40 is located in the vertical direction and at the center of the heating wire 32, thus achieving the purpose of drawing the tip.

[0031] In one implementation, such as Figure 2 and Figure 3 As shown, the system also includes a first check valve assembly 71 and a second check valve assembly 72 located above the first limiting structure 11. The check valve position of the second check valve assembly 72 is higher than that of the first check valve assembly 71. Furthermore, the two check valve assemblies are set in opposite directions. The first check valve assembly 71 restricts the upward movement of the first clamping assembly 2 to ensure system safety. The second check valve assembly 72 restricts the downward movement of the first clamping assembly 2. At this point, the first clamping assembly 2 has moved upward and is no longer restricted by the first check valve assembly 71. The second check valve assembly 72 ensures that the first clamping assembly 2 remains in a relatively high position after the pulling process is completed, facilitating subsequent operations such as removing the glass tube 40. It should be noted that both the first check valve assembly 71 and the second check valve assembly 72 should have a function to release the check valve, allowing for adjustment of the position of the first clamping assembly 2 as needed.

[0032] In one implementation, such as Figure 2 and Figure 3 As shown, it also includes a top limiting structure 13. The top limiting structure 13 can be the same limiting block as the first limiting structure 11 and the second limiting structure 12. The top limiting structure 13 is installed on the vertical guide rail 1 to limit or prevent the first clamping assembly 2 from coming off the vertical guide rail 1 upward.

[0033] In one implementation, such as Figures 5-7As shown, the first clamping assembly 2 includes a first slider 21, a first locking block 23, and a first clamping plate 22. The first clamping plate 22 is connected to the first slider 21, and the first slider 21 is slidably disposed on the vertical guide rail 1. When the first slider 21 slides along the vertical guide rail 1, it can drive the first clamping plate 22 to move vertically. The first clamping plate 22 is provided with a first V-shaped groove for fixing the glass tube 40. The first locking block 23 is alternately connected to both sides of the first V-shaped groove. Thus, the first locking block 23 can be used to press and fix the glass tube 40 in the first V-shaped groove. At the same time, the first V-shaped groove can accurately position the glass tube 40. A rubber gasket can also be provided on the side of the first locking block 23 that contacts the glass tube 40 to avoid damage to the glass tube 40 when it is pressed. The first locking block 23 can be in the form of a screw. When the first locking block 23 is rotated, the first clamping plate 22 can be loosened or screwed in, thereby adjusting the clamping force. The staggered arrangement can improve the clamping stability of the glass tube 40.

[0034] In one implementation, such as Figures 5-7 As shown, the second clamping assembly 4 includes a second slider 41, a second locking block 43, and a second clamping plate 42. The second clamping plate 42 is connected to the second slider 41, and the second slider 41 is slidably disposed on the vertical guide rail 1. When the second slider 41 slides along the vertical guide rail 1, it can drive the second clamping plate 42 to move vertically. The second clamping plate 42 is provided with a second V-shaped groove for fixing the glass tube 40. The second locking block 43 is alternately connected to both sides of the second V-shaped groove. Thus, the glass tube 40 can be squeezed and fixed in the second V-shaped groove by the second locking block 43. At the same time, the second V-shaped groove can accurately position the glass tube 40. A rubber gasket can also be provided on the side of the second locking block 43 that contacts the glass tube 40 to avoid damage to the glass tube 40 when squeezing it. The second locking block 43 can be in the form of a screw. When the second locking block 43 is rotated, the second clamping plate 42 can be loosened or screwed in, thereby adjusting the clamping force. The staggered arrangement can improve the clamping stability of the glass tube 40.

[0035] In one implementation, such as Figures 5-7As shown, the assembly also includes a first fine-tuning structure 24 and a second fine-tuning structure 44. A first clamping plate 22 is connected to a first slider 21 via the first fine-tuning structure 24. The bottom of the first clamping plate 22 abuts against the heating component 3. Adjusting the first fine-tuning structure 24 adjusts the distance between the first slider 21 and the heating component 3, thereby adjusting the height difference of the heating component 3. A second clamping plate 42 is connected to a second slider 41 via the second fine-tuning structure 44. The top of the second clamping plate 42 abuts against the heating component 3. Adjusting the second fine-tuning structure 44 adjusts the distance between the second slider 41 and the heating component 3, thereby adjusting the overall height difference of the second clamping assembly 4. Through the aforementioned height difference adjustment of the heating component 3 and the second clamping assembly 4, adjustments can be made according to the drawing requirements of different glass tubes 40, satisfying the drawing needs of glass tubes 40 of various specifications.

[0036] In one implementation, such as Figures 2-4 As shown, it also includes a limit switch 8, which is located near the second limiting structure 12. When the second clamping component 4 descends to the position of the second limiting structure 12, the limit switch 8 can detect the corresponding signal. Thus, the limit switch 8 can obtain the position state of the second clamping component 4 descending to the second limiting structure 12, marking the end of the drawing process. Based on this, the heating component 3 can be stopped from heating, realizing the automated control of the preparation module 10 by the control module 20.

[0037] In one implementation, such as Figures 2-4 As shown, it also includes a counterweight block 9, which includes multiple counterweight plates. The counterweight plates are detachably connected to the bottom of the second clamping plate 42 by bolts. The number of counterweight plates installed can be adjusted according to different counterweight requirements. For example, for a glass tube 40 with a thicker wall, it is necessary to increase the weight of the counterweight block 9 to increase the pulling force. In this case, the number of counterweight plates needs to be increased. Conversely, the number of counterweight plates is reduced. In addition, the taper of the pulling tip can be controlled by adjusting the number of counterweight plates of the counterweight block 9.

[0038] Combination Figures 1-8 This utility model provides a microelectrode pulling method, which can be applied to the microelectrode pulling instrument described above, and includes the following: The glass tube 40 is clamped between the first clamping assembly 2 and the second clamping assembly 4. The heating assembly 3 is located between the first clamping assembly 2 and the second clamping assembly 4. The first clamping assembly 2 is limited to an initial height position by the first limiting structure 11. The second clamping assembly 4 and the second limiting structure 12 have a certain falling height.

[0039] When the heating component 3 is turned on, the first clamping component 2 clamps the upper end of the glass tube 40, the heating component 3 heats the pulling area, the second clamping component 4 clamps the lower end of the glass tube 40 and pulls the glass tube 40 downward. At the same time, the heating component 3 moves synchronously under the action of gravity to match the change of the pulling area.

[0040] During one-step drawing, the second clamping component 4 descends directly to the position of the second limiting structure 12, and the heating component 3 descends to the position of the third limiting structure 5. One-step drawing is suitable for drawing electrodes with a long taper, such as adsorption electrodes for large viruses.

[0041] During the two-step drawing process, the second clamping component 4 first descends to the position of the fourth limiting structure 6, and the heating component 3 descends to the position of the third limiting structure 5. After a heating delay, the fourth limiting structure 6 is released, allowing the second clamping component 4 to continue descending to the position of the second limiting structure 12. The two-step drawing process is suitable for drawing electrodes with shorter tapers, such as diaphragm clamps.

[0042] This utility model provides two drawing methods, namely, one-step drawing and two-step drawing, as follows: The adjustment parameters used in the one-step drawing mode are mainly the heating value of the heating component 3 and the weight of the counterweight 9. The higher the heating value, the longer the electrode taper and the smaller the opening; the heavier the counterweight, the shorter the electrode taper and the larger the opening.

[0043] One-step drawing process: ① Install glass tube 40, determine the counterweight and install it.

[0044] ② The control module 20 displays the one-step drawing operation mode, determines the heating amount (P) and safety time (T) and adjusts them.

[0045] ③ Press the “START” button of the control module 20, the electromagnetic structure 61 of the fourth limit structure 6 is energized, the locking tongue 62 retracts, the heating wire 32 of the heating component 3 starts to heat, after the glass tube 40 is pulled open by the second clamping component 4, the second clamping component 4 slides down to trigger the limit switch 8, the heating wire 32 stops heating, the electromagnetic structure 61 is de-energized, the locking tongue 62 extends, and the pulling ends.

[0046] ④ Wait for the heating wire 32 to cool down, press the first check component 71, push the first clamping component 2 to the top, and remove the microelectrode.

[0047] In the two-step pulling mode, the adjustable parameters are: the first-step heating value of the heating component 3, the second-step heating value of the heating component 3, the weight of the counterweight 9, and the travel distance of the second clamping component 4. Among them, the higher the first-step heating value, the shorter the electrode taper and the larger the opening; the higher the second-step heating value, the longer the electrode taper and the smaller the opening; the heavier the counterweight, the shorter the electrode taper and the larger the opening; the greater the travel distance, the longer the electrode taper and the smaller the opening.

[0048] Two-step drawing process: ① Install glass tube 40, determine the counterweight and install it.

[0049] ② The control module 20 displays the two-step pulling operation mode, determines and adjusts the two-step heating amount (P), delayed heating time (the interval between the end of the first step and the start of the second step) and safety time (T).

[0050] ③ Press the “START” button on the control module 20. The first step of pulling begins, the heating wire 32 starts heating, and the glass tube 40 is pulled down by the second clamping component 4 by a distance. The second clamping component 4 touches the locking tongue 62 of the fourth limiting structure 6 and stops moving. At the same time, the heating component 3 moves down a distance, and the side wing of the ceramic base 31 of the heating component 3 touches the third limiting structure 5. The heating wire 32 stops heating, and the delayed heating time starts to start.

[0051] ④ When the delayed heating time is reached, the second pulling step begins. The heating wire 32 starts heating, the electromagnetic structure 61 of the fourth limiting structure 6 is energized, the locking tongue 62 retracts, and after the glass tube 40 is pulled open by the second clamping component 4, the second clamping component 4 slides down to trigger the limit switch 8, the heating wire 32 stops heating, the electromagnetic structure 61 is de-energized, the locking tongue 62 extends, and the pulling ends.

[0052] ⑤ Wait for the heating wire 32 to cool down, press the first check component 71, push the first clamping component 2 to the top, and remove the microelectrode.

[0053] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A microelectrode pulling device, characterized in that, include: A vertical guide rail, on which a first limiting structure and a second limiting structure are provided; A first clamping assembly is slidably disposed on the vertical guide rail and located above the first limiting structure; The second clamping assembly is slidably disposed on the vertical guide rail and located between the first limiting structure and the second limiting structure; A heating component is slidably disposed on the vertical guide rail and located between the first clamping component and the second clamping component. The heating component moves with the second clamping component under the action of gravity. And a third limiting structure, which is located below the heating component and is used to limit the low position of the heating component.

2. The microelectrode pulling device according to claim 1, characterized in that: The third limiting structure includes a limiting seat and a micro switch. The micro switch is disposed on the limiting seat and is used to obtain the position information of the heating component. The limiting seat is used to limit the low position of the heating component.

3. The microelectrode pulling device according to claim 2, characterized in that: It also includes a fourth limiting structure, which is located between the second clamping component and the second limiting structure, and has a retractable limiting part for limiting the stop position of the second clamping component.

4. The microelectrode pulling device according to claim 3, characterized in that: The fourth limiting structure includes an electromagnetic structure and a locking tongue. The electromagnetic structure is used to control the extension and retraction of the locking tongue, and the locking tongue is used to limit the stop position of the second clamping assembly.

5. The microelectrode pulling device according to claim 3, characterized in that: It also includes a first check component and a second check component located above the first limiting structure. The check position of the second check component is higher than that of the first check component. The first check component is used to restrict the first clamping component from moving upward, and the second check component is used to restrict the first clamping component from moving downward.

6. The microelectrode pulling device according to claim 3, characterized in that: The first clamping assembly includes a first slider, a first locking block, and a first clamping plate. The first clamping plate is connected to the first slider, and the first slider is slidably disposed on the vertical guide rail. The first clamping plate is provided with a first V-shaped groove for fixing the glass tube, and the first locking block is alternately connected to both sides of the first V-shaped groove. The second clamping assembly includes a second slider, a second locking block, and a second clamping plate. The second clamping plate is connected to the second slider, and the second slider is slidably disposed on the vertical guide rail. The second clamping plate is provided with a second V-shaped groove for fixing the glass tube, and the second locking block is alternately connected to both sides of the second V-shaped groove.

7. The microelectrode pulling device according to claim 6, characterized in that: It also includes a first fine-tuning structure and a second fine-tuning structure. The first clamping plate is connected to the first slider through the first fine-tuning structure, and the bottom of the first clamping plate is used to abut against the heating component. The second clamping plate is connected to the second slider through the second fine-tuning structure, and the top of the second clamping plate is used to abut against the heating component.

8. The microelectrode pulling device according to claim 6, characterized in that: It also includes a limit switch, which is located near the second limiting structure and is used to detect a signal that the second clamping assembly has descended to the second limiting structure.

9. The microelectrode pulling device according to claim 6, characterized in that: It also includes a counterweight block, which comprises multiple counterweight plates, and the counterweight plates are detachably connected to the bottom of the second clamping plate by bolts.

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