Exosome microfluidic isolation kit

Abstract

The utility model discloses an exosome micro -fluidic separation kit, including box body, the box body is equipped with test tube placement mixing mechanism in, the reagent bottle is placed in the box body, the box body top is equipped with protection mechanism, test tube placement mixing mechanism includes test tube stand, a plurality of guide rods are fixedly connected with test tube stand bottom, a plurality of guide rod bottom fixedly connected with the bottom plate, a plurality of test tube grooves are seted up on the test tube stand, belong to medical instrument technical field. The exosome micro -fluidic separation kit, through with the test tube that installs the magnetic bead is inserted to the test tube groove inside, drive motor moves the movable plate, and the movable plate moves and drives annular magnet to move, so that annular magnet moves ceaselessly up and down in the test tube periphery, and the magnetic bead in test tube is also moved up and down by attraction, makes the magnetic bead and sample fully mix, effectively improves the separation effect, compared with traditional centrifugal separation mode more quickly, effectively improves separation efficiency.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to an exosome microfluidic separation kit. Background Technology

[0002] Exosomes are tiny membrane vesicles released by cells, typically ranging from 30 to 150 nanometers in diameter. These vesicles contain a variety of bioactive molecules, including proteins, nucleic acids, and lipids, which play important roles in intercellular communication. In current biomedical research, stem cell exosomes have attracted much attention due to their unique biological functions and potential targets for disease diagnosis and treatment. However, efficiently and purely isolating exosomes from complex biological samples remains a major challenge. Traditional separation methods, such as ultracentrifugation, ultrafiltration, and density gradient centrifugation, while capable of separating exosomes to some extent, often suffer from problems such as cumbersome operation, long processing time, low recovery rate, and potential introduction of exogenous contamination, limiting their widespread application in clinical research. Utility Model Content

[0003] To address the shortcomings of existing technologies, this invention provides an exosome microfluidic separation kit, which solves the aforementioned problems.

[0004] To achieve the above objectives, this utility model is implemented through the following technical solution: an exosome microfluidic separation kit, comprising a box body, wherein the box body is provided with a test tube placement and mixing mechanism, a reagent bottle is placed inside the box body, and a protective mechanism is provided on the top of the box body; The test tube placement and mixing mechanism includes a test tube rack, with multiple guide rods fixedly connected to the bottom of the test tube rack. A base plate is fixedly connected to the bottom end of each guide rod. Multiple test tube slots are provided on the test tube rack. Movable plates are slidably connected to each guide rod. Multiple annular magnets are fixedly connected to the movable plates. A top frame is fixedly connected to the center of the movable plates. A guide seat is provided on one side of the top frame. A guide slide is provided inside the guide seat. A top rod is provided between the guide slide and the top frame. A driven rod is rotatably connected to one side surface of the guide slide. A drive rod is rotatably connected to one end of the driven rod, and a drive motor is fixedly connected to the end of the drive rod.

[0005] Preferably, the base plate is fixedly installed on the inner wall of the box, and multiple rubber rings are provided on the inner wall of the test tube groove to improve the stability of the test tube.

[0006] Preferably, the plurality of annular magnets correspond one-to-one with the plurality of test tube troughs, and the annular magnets are snapped into the movable plate so that the annular magnets can attract the magnetic beads inside the test tube troughs.

[0007] Preferably, one end of the top rod is rotatably connected to the top frame, and the other end of the top rod is rotatably connected to the guide slide, so that when the guide slide moves, it can drive the top rod to move, thereby lifting the top frame.

[0008] Preferably, the end of the guide seat is fixedly connected to the base plate, and the guide slide is slidably connected to the guide seat, so that the guide slide can slide along the guide seat.

[0009] Preferably, the output shaft end of the drive motor is connected to one end of the drive rod, and the drive motor is provided with a support frame, which can effectively fix the drive motor and make the drive motor stable.

[0010] Preferably, the protective mechanism includes a top cover, with limiting ropes fixedly connected to both sides of the top cover, and limiting balls fixedly connected to the ends of the two limiting ropes. The limiting balls are disposed inside the box body, and the limiting ropes pass through the box body. A lighting lamp is installed inside the top cover, which can protect the box body, support the top cover, and provide convenient lighting.

[0011] This invention provides an exosome microfluidic separation kit. Compared with the prior art, it has the following advantages: 1. This exosome microfluidic separation kit works by inserting a test tube containing magnetic beads into a test tube slot. A drive motor moves a movable plate, which in turn moves a ring magnet. The ring magnet moves up and down around the outside of the test tube, attracting the magnetic beads inside the test tube to move up and down as well. This allows the magnetic beads to mix thoroughly with the sample, effectively improving the separation effect. Compared with traditional separation methods such as centrifugation, this method is faster and more efficient.

[0012] 2. This exosome microfluidic separation kit allows for operation by pulling the top cover, which in turn moves the limiting rope, which in turn moves the limiting ball. The limiting ball then holds the limiting rope in place, providing support for the top cover. Simultaneously, a light can be used for illumination, facilitating operation by staff. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the test tube placement and mixing mechanism of this utility model; Figure 3 This is a schematic diagram of the guide seat structure of this utility model; Figure 4 This is a schematic diagram of the protective mechanism structure of this utility model.

[0014] In the diagram: 1. Box body; 2. Test tube placement and mixing mechanism; 201. Test tube rack; 202. Guide rod; 203. Base plate; 204. Test tube trough; 205. Movable plate; 206. Ring magnet; 207. Top frame; 208. Guide seat; 209. Guide slide; 210. Top rod; 211. Drive rod; 212. Drive rod; 213. Drive motor; 3. Reagent bottle; 4. Protective mechanism; 401. Top cover; 402. Limiting rope; 403. Limiting ball; 404. Lighting lamp. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figure 1-3 This utility model provides a technical solution: an exosome microfluidic separation kit, which uses magnetic beads to separate exosomes. The kit includes a housing 1 with a built-in power supply. Operation buttons are conveniently located on the outside of the housing 1. Inside the housing 1 is a test tube placement and mixing mechanism 2, and a reagent bottle 3 is placed inside. A protective mechanism 4 is located on the top of the housing 1. The test tube placement and mixing mechanism 2 mixes the test tubes containing magnetic beads, ensuring continuous movement and thorough mixing with the sample, effectively improving the separation effect. Compared to traditional separation methods such as centrifugation, this method is faster and significantly improves separation efficiency.

[0017] The test tube placement and mixing mechanism 2 includes a test tube rack 201. Multiple guide rods 202 are fixedly connected to the bottom of the test tube rack 201, and a base plate 203 is fixedly connected to the bottom of each guide rod 202. Multiple test tube slots 204 are provided on the test tube rack 201. The base plate 203 is fixedly installed on the inner wall of the box body 1. Multiple rubber rings are provided on the inner wall of each test tube slot 204 to improve the stability of the test tubes. Movable plates 205 are slidably connected to each of the guide rods 202. Multiple annular magnets 206 are fixedly connected to each movable plate 205, and each annular magnet 206 corresponds one-to-one with a test tube slot 204. The annular magnets 206 are engaged with the movable plates 205, allowing them to attract the magnetic beads inside the test tube slots 204. A top frame 207 is fixedly connected to the center of the movable plate 205. A guide seat 208 is provided on one side of the top frame 207, and a guide slide 209 is provided inside the guide seat 208. The end of the seat 208 is fixedly connected to the base plate 203, and the guide slide 209 is slidably connected to the guide seat 208, so that the guide slide 209 can slide along the guide seat 208. A top rod 210 is provided between the guide slide 209 and the top frame 207. One end of the top rod 210 is rotatably connected to the top frame 207, and the other end of the top rod 210 is rotatably connected to the guide slide 209, so that when the guide slide 209 moves, it can drive the top rod 210 to move, thereby lifting the top frame 207. A driven rod 211 is rotatably connected to one side surface of the guide slide 209. A drive rod 212 is rotatably connected to one end of the driven rod 211. A drive motor 213 is fixedly connected to the end of the drive rod 212. The output shaft end of the drive motor 213 is connected to one end of the drive rod 212. A support frame is provided on the drive motor 213, which can effectively fix the drive motor 213 and make the drive motor 213 stable.

[0018] Please see Figure 1 and Figure 4 The protective mechanism 4 includes a top cover 401. Limiting ropes 402 are fixedly connected to both sides of the top cover 401. Limiting balls 403 are fixedly connected to the ends of the two limiting ropes 402. The limiting balls 403 are located inside the box body 1, and the limiting ropes 402 pass through the box body 1. A lighting lamp 404 is installed inside the top cover 401. By pulling the top cover 401, the movement of the top cover 401 moves the limiting ropes 402, and the movement of the limiting ropes 402 moves the limiting balls 403. The limiting balls 403 hold the limiting ropes 402, providing support for the top cover 401. At the same time, the lighting lamp 404 provides illumination, making it convenient for workers to operate.

[0019] During operation, a test tube containing magnetic beads is inserted into the test tube slot 204. The drive motor 213 drives the drive rod 212 to rotate, which in turn drives the driven rod 211 to rotate. The driven rod 211 moves, pushing the guide slide 209 to reciprocate along the guide seat 208. The movement of the guide slide 209 drives the top rod 210 to move, which in turn drives the top frame 207 to move up and down. The movement of the top frame 207 drives the movable plate 205 to move, which in turn drives the annular magnet 206 to move. This causes the annular magnet 206 to move up and down around the test tube, attracting the magnetic beads inside the test tube to move up and down as well, allowing the magnetic beads to mix thoroughly with the sample. This effectively improves the separation effect and is faster than traditional separation methods such as centrifugation, thus significantly improving separation efficiency.

[0020] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

Claims

1. An exosome microfluidic separation kit, comprising a housing (1), characterized in that: The box (1) is equipped with a test tube placement and mixing mechanism (2) inside, a reagent bottle (3) is placed inside the box (1), and a protective mechanism (4) is provided on the top of the box (1). The test tube placement and mixing mechanism (2) includes a test tube rack (201), with multiple guide rods (202) fixedly connected to the bottom of the test tube rack (201). A base plate (203) is fixedly connected to the bottom of each guide rod (202). Multiple test tube slots (204) are provided on the test tube rack (201). Movable plates (205) are slidably connected to each guide rod (202). Multiple annular magnets (206) are fixedly connected to the movable plates (205). The central part of the movable plate (205)... A top frame (207) is fixedly connected to the top frame (207). A guide seat (208) is provided on one side of the top frame (207). A guide slide (209) is provided inside the guide seat (208). A top rod (210) is provided between the guide slide (209) and the top frame (207). A driven rod (211) is rotatably connected to one side surface of the guide slide (209). A drive rod (212) is rotatably connected to one end of the driven rod (211). A drive motor (213) is fixedly connected to the end of the drive rod (212).

2. The exosome microfluidic separation kit according to claim 1, characterized in that: The base plate (203) is fixedly installed on the inner wall of the box (1), and multiple rubber rings are provided on the inner wall of the test tube groove (204).

3. The exosome microfluidic separation kit according to claim 1, characterized in that: The multiple ring magnets (206) correspond one-to-one with the multiple test tube slots (204), and the ring magnets (206) are snapped into the movable plate (205).

4. The exosome microfluidic separation kit according to claim 1, characterized in that: One end of the top rod (210) is rotatably connected to the top frame (207), and the other end of the top rod (210) is rotatably connected to the guide slide (209).

5. The exosome microfluidic separation kit according to claim 1, characterized in that: The end of the guide seat (208) is fixedly connected to the base plate (203), and the guide slide (209) is slidably connected to the guide seat (208).

6. The exosome microfluidic separation kit according to claim 1, characterized in that: The output shaft end of the drive motor (213) is connected to one end of the drive rod (212), and the drive motor (213) is provided with a support frame.

7. The exosome microfluidic separation kit according to claim 1, characterized in that: The protective mechanism (4) includes a top cover (401), with limit ropes (402) fixedly connected to both sides of the top cover (401), and limit balls (403) fixedly connected to the ends of the two limit ropes (402).

8. The exosome microfluidic separation kit according to claim 7, characterized in that: The limiting ball (403) is located inside the box (1), and the limiting rope (402) is connected through the box (1). A lighting lamp (404) is installed inside the top cover (401).

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