Reef membrane gamete efficient collection and culture device

Abstract

The utility model discloses a kind of high-efficiency collection and culture devices of reef membrane gamete, belong to aquaculture technical field;Including collection tank and culture tank, the upper surface of the collection tank is provided with a plurality of slot one, the upper surface of the culture tank is provided with a plurality of slot two, the inside of multiple slot one and the inside of multiple slot two are all provided with attachment plate;The utility model does not need to rely on outdoor natural environment, can effectively avoid wind and rain, temperature sudden change and other natural factors on the interference of gamete combination, sporozoite growth, provide stable controllable environment for reef membrane cultivation, simultaneously, the collection tank and culture tank used by device are all PET board material with good light transmission, can fully meet the demand of reef membrane growth to illumination, and PET material itself is light in texture, collocation attachment plate, overall weight is moderate, whether it is the plug-in, transfer of attachment plate, or the movement, cleaning of tank body, operating personnel can easily complete without the aid of complex tool.

Description

Technical Field

[0001] This utility model relates to the field of aquaculture technology, and in particular to a device for efficient collection and cultivation of reef gametes. Background Technology

[0002] Reef seaweed (also known as green laver, green tower seaweed, paper seaweed, etc.) is a type of large green edible economic seaweed widely distributed along the coasts of the world. It is also a common raw material for medicine and cosmetics, and has important ecological functions. This type of algae is rich in high-quality protein, a variety of vitamins and minerals, low in fat and high in nutritional value, making it a typical green and healthy food. In addition to its edible value, reef seaweed has a wide range of uses in traditional industries such as agriculture, food, industry, medicine, and cosmetics. In recent years, it has also been widely studied for use in emerging fields such as marine bioremediation, carbon sequestration and green energy, and has extremely high development potential.

[0003] Currently, reef algae has been commercially cultivated on a certain scale in Japan and South Korea. Reef algae is the most widely cultivated type of green algae, accounting for about 90% of the global green algae cultivation. Most of the products are used in the Japanese food market. However, the domestic production of reef algae in Japan is still insufficient to meet market demand, and it needs to rely on imports to supplement the supply. my country is an important seaweed cultivation country in the world. Achieving localized large-scale cultivation of reef algae can not only meet the domestic consumer market, but also has strong export potential. However, the artificial cultivation of reef algae in my country is still in its initial stage. Only a small-scale experimental exploration has been carried out in a few coastal areas such as Guangdong and Zhejiang. An industrialization system and standardized production system have not yet been formed.

[0004] Unlike seaweeds such as Euphorbia milii and Asparagus cochinchinensis, which mainly rely on vegetative propagation to provide seedlings, the artificial cultivation of reef seaweed requires the cultivation of sporangium seedlings. Currently, the mainstream source of seedlings for reef seaweed cultivation internationally is still natural collection. Typically, during the autumn high tide, net curtains are set up in the sea area to collect zoospores released from naturally overwintering sporangiums. However, this method is limited by the availability of suitable sea areas for collection, and the seedling attachment effect is greatly affected by annual environmental factors, resulting in unstable seedling production. Japan began artificial seedling cultivation trials of reef seaweed in the 1990s and applied them to production. However, my country's research on artificial seedling cultivation is still in the experimental stage, and the technical bottlenecks of large-scale artificial sporangium cultivation and artificial zoospore collection have not yet been effectively overcome. Therefore, how to standardize and scale up artificial seedling cultivation of reef seaweed is a key technology that urgently needs to be tackled in the field of artificial seedling cultivation and cultivation of reef seaweed. Utility Model Content

[0005] Purpose of the utility model: The purpose of this utility model is to provide a highly efficient reef gamete collection and culture device that has high seedling attachment efficiency, more uniform light exposure, consistent seedling growth, convenient management, is not easily contaminated by miscellaneous algae, and is easy to observe and manage.

[0006] Technical solution: A device for efficient collection and cultivation of reef gametes, comprising a collection tank and a cultivation tank, wherein the upper surface of the collection tank is provided with a plurality of slot 1, and the upper surface of the cultivation tank is provided with a plurality of slot 2, and an attachment plate is provided inside the plurality of slot 1 and the plurality of slot 2.

[0007] In use, the attachment plate is inserted into the collection tank and placed in the reef film gamete solution. After being treated in the dark for 4 to 8 hours, the zygotes formed by the combination of male and female gametes are evenly attached to the surface of the attachment plate. Then the attachment plate is transferred to the culture tank and cultured continuously for 5 to 7 months in a suitable environment to complete the stable growth and development of the sporophyte.

[0008] Furthermore, both the collection tank and the culture tank are made of PET material with good light transmittance and a thickness of 2mm.

[0009] Furthermore, the plurality of card slots one are spaced 3mm apart, and the plurality of card slots two are spaced 18.16mm apart.

[0010] Furthermore, 110 attachment plates can be inserted into the interior of the plurality of slots one, and 24 attachment plates can be inserted into the interior of the plurality of slots two.

[0011] Furthermore, both the bottom of the collection tank and the culture tank are fixedly connected with multiple protrusions.

[0012] Furthermore, the height of each of the aforementioned protrusions is 1 cm.

[0013] Furthermore, all of the aforementioned attachment plates are made of PET material, with a thickness of 1mm to 1.2mm, a length of 20cm, and a width of 10cm.

[0014] Furthermore, the surfaces of all of the aforementioned attachment plates are roughened.

[0015] Furthermore, both the collection tank and the culture tank are cuboid structures, and the plurality of attachment plates are rectangular thin plates.

[0016] Beneficial effects: This invention does not rely on the outdoor natural environment and can effectively avoid the interference of natural factors such as wind, rain and sudden temperature changes on gamete combination and sporophyte growth, providing a stable and controllable environment for reef membrane cultivation. At the same time, the collection tank and culture tank used in the device are made of PET sheets with good light transmittance, which can fully meet the light requirements of reef membrane growth. Moreover, the PET material itself is lightweight, and with the attachment plate, the overall weight is moderate. Whether it is the insertion, removal and transfer of the attachment plate, or the movement and cleaning of the tank, the operator can easily complete it without the help of complicated tools, which greatly reduces the difficulty of daily operation and management and improves the convenience of the cultivation process.

[0017] The core components of this utility model only include a collection tank, a culture tank, and an attachment plate. The components are connected or supported by slots and protrusions. There are no complicated assembly nodes or precision parts. Even if the operator lacks professional experience in operating biological cultivation equipment, he or she can quickly master the device’s construction method and usage process through simple guidance. This effectively reduces the operating threshold and learning cost, and is suitable for promotion and use in seedling cultivation scenarios of different scales.

[0018] The attachment plate of this invention undergoes double-sided grinding and roughening treatment, which increases the surface friction and attachment area, allowing zygotes to adhere more firmly to the plate surface and reducing cultivation losses caused by poor adhesion. The spacing between the slots in the collection tank is precisely controlled, which can achieve an orderly arrangement of the attachment plates without affecting the flow of gamete liquid due to excessive spacing. At the same time, the tank body retains gaps through 1cm high protrusions, which can ensure smooth circulation of gamete liquid or culture water in the tank, avoiding local water accumulation and uneven nutrient distribution. This provides a uniform attachment environment and sufficient nutrient supply for reef zygotes, helping them to develop smoothly, thereby greatly improving the collection and attachment efficiency of the device.

[0019] This invention ensures that all gametes collected from the same batch of algae in each collection originate from the same source, guaranteeing genetic consistency and quality stability of the gametes from the outset. This avoids growth differences caused by mixed gamete sources. Furthermore, the entire cultivation process is conducted in a controlled indoor environment, maintaining uniform key cultivation conditions such as temperature, light intensity, and water flow rate. The cultivation cycle is fixed at 5–7 months, preventing fluctuations or changes in cultivation conditions due to external factors. Ultimately, this ensures a high degree of consistency in the growth progress, morphological characteristics, and health status of each batch of reef sporophytes. This facilitates unified daily monitoring, nutrient supplementation, and pest and disease control for operators, reducing management challenges caused by individual growth differences and improving the standardization of cultivation management.

[0020] This invention features a simple overall structure, facilitating standardized mass production and significantly reducing equipment investment costs per seedling stage. It is suitable for large-scale application. Furthermore, the PET material possesses excellent seawater corrosion resistance and oxidation resistance, along with high toughness and durability, allowing the device to be reused over multiple seedling cycles. This reduces equipment replacement frequency and resource waste, lowering long-term seedling costs. The standardized materials, structure, and cultivation process promote a standardized system for indoor reef membrane seedling cultivation, preventing instability in cultivation quality due to equipment or operational differences. This provides strong support for the industrialization and standardization of reef membrane seedling cultivation. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall assembly structure of the collection slot and the attachment plate of this utility model;

[0022] Figure 2This is a schematic diagram of the overall assembly structure of the collection slot of this utility model;

[0023] Figure 3 This is a schematic diagram of the overall assembly structure of the culture tank and the attachment plate of this utility model;

[0024] Figure 4 This is a schematic diagram of the overall assembly structure of the culture tank of this utility model.

[0025] In the diagram: 1. Collection tank; 2. Culture tank; 3. Slot 1; 4. Slot 2; 5. Attachment plate; 6. Protrusion. Detailed Implementation

[0026] To make the technical solution of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0027] Example

[0028] like Figures 1-4 As shown, a device for efficient collection and cultivation of reef gametes is provided, including a collection tank 1 and a cultivation tank 2. The upper surface of the collection tank 1 is provided with multiple slots 3, and the upper surface of the cultivation tank 2 is provided with multiple slots 4. An attachment plate 5 is provided inside the multiple slots 3 and the multiple slots 4. Both the collection tank 1 and the cultivation tank 2 are cuboid structures, and the multiple attachment plates 5 are rectangular thin plates.

[0029] In use, the attachment plate 5 is inserted into the collection tank 1 and placed in the reef film gamete solution. After being treated in the dark for 4 to 8 hours, the zygotes formed by the combination of male and female gametes are evenly attached to the surface of the attachment plate 5. Then, the attachment plate 5 is transferred to the culture tank 2 and cultured continuously for 5 to 7 months under suitable conditions. After the sporangia mature and release zoospores, they are attached to the seedling curtain and hung in the sea area for cultivation during high tide. This completes the stable growth and development of the sporophyte, thus enabling efficient collection and simultaneous cultivation of a large number of reef film sporophytes with consistent growth status. It has significant potential for standardized application in the large-scale artificial seedling cultivation of reef films.

[0030] like Figures 1-4 As shown, both the collection tank 1 and the culture tank 2 are made of PET material with good light transmittance and a thickness of 2mm.

[0031] Multiple attachment plates 5 are made of PET material with a thickness of 1mm to 1.2mm, a length of 20cm and a width of 10cm; the surfaces of multiple attachment plates 5 are roughened.

[0032] Both the collection tank 1 and the culture tank 2 are made of 2mm thick PET sheets with excellent light transmittance. This material is submerged in water, non-toxic, and ensures a safe culture environment. Its light transmittance facilitates observation and meets the biological light requirements. At the same time, it is resistant to seawater corrosion, oxidation, and has good elasticity and structural stability, enabling durability and reducing maintenance costs. Meanwhile, multiple attachment plates 5 are also made of PET material, with a thickness of 1mm to 1.2mm and a size of 20cm × 10cm. Their thin and regular size allows for flexible arrangement in the culture space without affecting water flow and light in the tank. Furthermore, PET material is low in cost, highly malleable, and tough, making it difficult to break. Its good light transmittance also allows the attachment plates to be directly placed under a microscope for observation, improving efficiency. At the same time, its surface is professionally roughened to increase friction and adhesion, providing a stable attachment environment for the zygotes and preventing zygote detachment from affecting the culture.

[0033] like Figures 1-4 As shown, the multiple card slots 3 are spaced 3mm apart, and the multiple card slots 4 are spaced 18.16mm apart; 110 attachment plates 5 can be inserted into the multiple card slots 3, and 24 attachment plates 5 can be inserted into the multiple card slots 4.

[0034] The multiple slots 1 and 3 are spaced 3mm apart, and can accommodate 110 attachment plates 5. The closer spacing allows for the orderly arrangement of more attachment plates within a limited space, meeting the needs of large-scale zygote attachment culture. At the same time, the tight and uniform spacing ensures that each attachment plate is stably fixed, avoiding shaking caused by excessive gaps. The multiple slots 2 and 4 are spaced 18.16mm apart, and can accommodate 24 attachment plates 5. The wider spacing provides more independent space for the attachment plates, reducing mutual interference between adjacent attachment plates. This is suitable for culture scenarios with higher requirements for growth space. The different spacing and capacity designs of the two slots can be flexibly selected according to actual culture needs, improving the applicability and practicality of the equipment.

[0035] like Figures 1-4 As shown, multiple protrusions 6 are fixedly connected to the bottom of both the collection tank 1 and the culture tank 2; the height of each of the multiple protrusions 6 is 1cm.

[0036] The multiple protrusions 6 are all 1cm high, which will not excessively occupy the internal space of the tank and affect the culture capacity, while effectively forming reasonable gaps at the bottom of the tank. This allows for smooth water flow in the tank, avoiding local water accumulation or stagnation. The smooth water flow not only provides a uniform attachment environment for the zygotes, reducing attachment differences caused by uneven water flow, but also allows the sporophytes to fully contact and absorb nutrients, promoting normal biological growth. At the same time, the gaps formed by the bottom protrusions 6 also reduce the accumulation of culture residues at the bottom of the tank, making it easier to thoroughly clean the tank later and reducing the difficulty of cleaning.

[0037] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A device for efficient collection and culture of reef gametes, comprising a collection tank (1) and a culture tank (2), characterized in that: The upper surface of the collection tank (1) is provided with multiple slots 1 (3), and the upper surface of the culture tank (2) is provided with multiple slots 2 (4). An attachment plate (5) is provided inside the multiple slots 1 (3) and the multiple slots 2 (4). When in use, the attachment plate (5) is inserted into the collection tank (1) and placed in the reef gamete solution. After being treated in the dark for 4 to 8 hours, the zygotes formed by the combination of male and female gametes are evenly attached to the surface of the attachment plate (5). Then the attachment plate (5) is transferred to the culture tank (2) and cultured continuously for 5 to 7 months in a suitable environment to complete the stable growth and development of the sporophyte.

2. The device for efficient collection and culture of reef gametes according to claim 1, characterized in that: Both the collection tank (1) and the culture tank (2) are made of PET material with good light transmittance and have a thickness of 2 mm.

3. The device for efficient collection and culture of reef gametes according to claim 1, characterized in that: The multiple card slots one (3) are spaced 3 mm apart, and the multiple card slots two (4) are spaced 18.16 mm apart.

4. The device for efficient collection and culture of reef membrane gametes according to claim 1, characterized in that: 110 attachment plates (5) can be inserted into the interior of the plurality of slots one (3), and 24 attachment plates (5) can be inserted into the interior of the plurality of slots two (4).

5. The device for efficient collection and cultivation of reef gametes according to claim 1, characterized in that: The bottom ends of the collection tank (1) and the culture tank (2) are both fixedly connected with multiple protrusions (6).

6. The device for efficient collection and culture of reef membrane gametes according to claim 5, characterized in that: The height of each of the multiple protrusions (6) is 1 cm.

7. The device for efficient collection and cultivation of reef gametes according to claim 1, characterized in that: The multiple attachment plates (5) are all made of PET material with a thickness of 1mm to 1.2mm, a length of 20cm and a width of 10cm.

8. The device for efficient collection and culture of reef membrane gametes according to claim 1, characterized in that: The surfaces of all of the attachment plates (5) are roughened.

9. The device for efficient collection and culture of reef gametes according to claim 1, characterized in that: Both the collection tank (1) and the culture tank (2) are cuboid structures, and the multiple attachment plates (5) are rectangular thin plates.

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