A 96 well plate to prevent cross contamination

Abstract

The utility model relates to 96 -well plate technical field, and specifically is a kind of 96 -well plate to prevent cross-contamination, including bottom plate, the bottom plate inner chamber is provided with clamping plate, the clamping plate inner chamber is provided with lofting pipe, the bottom plate top is provided with cover plate, the cover plate inner wall top is provided with sealing mechanism, and the sealing mechanism includes locating rod;The utility model is by introducing cell reagent into the lofting pipe on clamping plate, using cover plate to be clamped on bottom plate, the sealing mechanism on cover plate blocks the port of lofting pipe, the plug on locating rod is clamped in the opening of lofting pipe, and the sealing ring in sealing groove is against the port of lofting pipe, prevent the cell reagent in it from overflowing, so that the cell reagent in lofting pipe does not overflow when oscillating or centrifuging, further reaches the effect that lofting pipe does not cross-contamination when oscillating or centrifuging, solves the problem that the hole between the hole on 96 -well plate is prone to cross-contamination, thereby leading to the problem of affecting detection result.

Description

Technical Field

[0001] This utility model relates to the field of 96-well plate technology, specifically a 96-well plate that prevents cross-contamination. Background Technology

[0002] 96-well plates, also known as 96-well cell culture plates, are made of optically transparent pure polystyrene using a special process to ensure optimal cell adsorption. They are mainly used for the growth and reproduction of animal and human cells, such as nerve cells and tumor cells. They are widely used by numerous research institutions and bioengineering companies.

[0003] Currently, in existing technologies, 96-well plates have 96 wells. When using them, reagents are added to each well. However, during the reagent addition process, due to the poor isolation between each well, cross-contamination can easily occur during shaking or centrifugation, which in turn affects the accuracy of the test results. Therefore, there is a need to propose a 96-well plate that prevents cross-contamination. Utility Model Content

[0004] To address the shortcomings of existing technologies, such as the problem that cross-contamination can easily occur between the wells on a 96-well plate, thus affecting the test results, this invention proposes a 96-well plate that prevents cross-contamination.

[0005] The technical solution adopted by this utility model to solve its technical problem is: a 96-well plate to prevent cross-contamination, including a base plate, a clamping plate is provided in the inner cavity of the base plate, a sample placement tube is provided in the inner cavity of the clamping plate, a cover plate is provided on the top of the base plate, and a sealing mechanism is provided on the top of the inner wall of the cover plate. The sealing mechanism includes a positioning rod, which is fixedly connected to the top of the inner wall of the cover plate. A plug is slidably connected to the outer wall of the positioning rod. A sealing groove is provided at the bottom of the plug, and a sealing ring is provided in the sealing groove. The sealing ring contacts the top of the sample tube. A rubber gasket is provided at the top of the plug, and the rubber gasket is slidably connected to the outer wall of the positioning rod. An outer edge is provided at the bottom of the plug, and the outer edge is inclined outward.

[0006] Preferably, the outer side of the base plate is provided with a side groove, the cover plate is inserted into the outside of the side groove, a positioning plate is fixedly connected to one side of the base plate, and a positioning groove is provided on one side of the cover plate, the positioning plate and the positioning groove are inserted into each other.

[0007] Preferably, the bottom plate has a through groove in its inner cavity, and the clamping plate is inserted into the inside of the through groove.

[0008] Preferably, the bottom of the through groove is provided with a circular groove, and one end of the sample-laying tube passes through the bottom of the clamp and extends into the interior of the circular groove.

[0009] Preferably, slots are provided on both sides of the through groove, and blocks are fixedly connected to both sides of the clamping plate, with the blocks inserted into the slots.

[0010] Preferably, the outer wall of the positioning rod is provided with a sliding groove, and one end of the plug is slidably connected inside the sliding groove.

[0011] Preferably, the plug has a limiting groove in its inner cavity, and the lower edge of the positioning rod is slidably connected inside the limiting groove.

[0012] The advantages of this utility model are: This invention introduces cell reagents into a sample tube on a clamping plate, uses a cover plate to hold it in place on a base plate, and a sealing mechanism on the cover plate to block the port of the sample tube. A plug on the positioning rod is engaged with the opening of the sample tube, and a sealing ring in the sealing groove abuts against the port of the sample tube to prevent the cell reagents from overflowing. This prevents the cell reagents in the sample tube from overflowing during shaking or centrifugation, thus preventing cross-contamination during shaking or centrifugation. This solves the problem of cross-contamination between wells on a 96-well plate, which can affect the test results. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a side sectional view of the structure of this utility model; Figure 3 This is a schematic diagram of the structure of the inner cavity of the base plate of this utility model; Figure 4 This utility model Figure 2 Enlarged structural diagram of section A in the middle; Figure 5 This utility model Figure 3 Enlarged structural diagram of section B in the middle.

[0015] In the diagram: 1. Base plate; 11. Positioning plate; 12. Through groove; 13. Circular groove; 14. Slot; 15. Side groove; 2. Clamping plate; 21. Laying tube; 22. Locking block; 3. Sealing mechanism; 31. Positioning rod; 32. Plug; 33. Rubber pad; 34. Sealing groove; 35. Sealing ring; 36. Outer edge; 37. Sliding groove; 38. Limiting groove; 4. Cover plate; 41. Positioning groove. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0017] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail. This application discloses a 96-well plate for preventing cross-contamination. (See also...) Figures 1-5 A 96-well plate for preventing cross-contamination includes a base plate 1, a clamping plate 2 inside the base plate 1, a sample tube 21 inside the clamping plate 2, a cover plate 4 on the top of the base plate 1, and a sealing mechanism 3 on the top of the inner wall of the cover plate 4. By introducing cell reagent into the sample tube 21, the clamping plate 2 and the sample tube 21 are stably inserted into the inner cavity of the base plate 1. The cover plate 4 is placed on top of the base plate 1, and the sealing mechanism 3 blocks the port of the sample tube 21, so that the cell reagent in the sample tube 21 will not overflow. Therefore, the cell reagent inside the sample tube 21 will not overflow during shaking and centrifugation, thus preventing cross-contamination. The sealing mechanism 3 includes a positioning rod 31, which is fixedly connected to the top of the inner wall of the cover plate 4. A plug 32 is slidably connected to the outer wall of the positioning rod 31. A sealing groove 34 is provided at the bottom of the plug 32, and a sealing ring 35 is provided in the sealing groove 34. The sealing ring 35 contacts the top of the placement tube 21. A rubber pad 33 is provided at the top of the plug 32, and the rubber pad 33 is slidably connected to the outer wall of the positioning rod 31. An outer edge 36 is provided at the bottom of the plug 32, and the outer edge 36 is inclined outward. By covering the cover plate 4 on top of the base plate 1, the positioning mechanism is achieved. The plug 32 on the rod 31 is engaged at the port of the sample tube 21. The inclined outer edge 36 can improve the fault tolerance of the connection between the plug 32 and the port of the sample tube 21. The rubber pad 33 above the plug 32 cooperates to abut against the plug 32 to improve the tightness of the contact between the plug 32 and the port of the sample tube 21, so that the plug 32 and the sample tube 21 can be sealed. The sealing ring 35 in the sealing groove 34 abuts against the port of the sample tube 21 to prevent the cell reagent in the sample tube 21 from overflowing, thus achieving the purpose of the plug 32 blocking the port of the sample tube 21.

[0018] Reference Figure 1 , Figure 3 and Figure 5The base plate 1 has a side groove 15 on its outer side, and the cover plate 4 is inserted into the outside of the side groove 15. A positioning plate 11 is fixedly connected to one side of the base plate 1, and a positioning groove 41 is opened on one side of the cover plate 4. The positioning plate 11 and the positioning groove 41 are inserted into each other. A circular groove 13 is opened at the bottom of the through groove 12. One end of the placement tube 21 passes through the bottom of the clamping plate 2 and extends into the inside of the circular groove 13. A slot 14 is opened on both sides of the through groove 12, and a locking block 22 is fixedly connected to both sides of the clamping plate 2. The locking block 22 is inserted into the inside of the slot 14 and passes through the edge of the cover plate 4. The cover plate 4 is inserted into the side groove 15 of the base plate 1. The cover plate 4 and the base plate 1 can be easily opened and closed through the positioning plate 11 and the positioning groove 41. The clamping plate 2 is inserted into the through groove 12. The bottom of the laying tube 21 extends into the circular groove 13. The clamping block 22 is inserted into the clamping groove 14. The clamping blocks 22 on both sides of the clamping plate 2 can provide a stable force point when the clamping plate 2 is disassembled and assembled, thereby improving the convenience of disassembling and assembling the clamping plate 2 and the laying tube 21. The clamping plate 2 and the laying tube 21 can be disassembled separately for cleaning, which improves the convenience of cleaning the laying tube 21.

[0019] Reference Figure 2 and Figure 4 The outer wall of the positioning rod 31 has a sliding groove 37. One end of the plug 32 is slidably connected to the inside of the sliding groove 37. The inner cavity of the plug 32 has a limiting groove 38. The lower edge of the positioning rod 31 is slidably connected to the inside of the limiting groove 38. The inner cavity of the base plate 1 has a through groove 12. The clamping plate 2 is inserted into the inside of the through groove 12. The plug 32 slides in the sliding groove 37 on the outer wall of the positioning rod 31. The rubber pad 33 cooperates with the plug 32 to improve the tightness of the contact between the plug 32 and the port of the sampling tube 21, so that the plug 32 and the sampling tube 21 can be sealed. The lower edge of the positioning rod 31 cooperates with the limiting groove 38 to make the plug 32 slide stably up and down.

[0020] Working principle: In use, the cell reagent is introduced into the sample tube 21 on the clamp 2. One end of the sample tube 21 extends into the circular groove 13. The cover plate 4 is placed over the sample tube 21 along the side groove 15 of the base plate 1. The sealing mechanism 3 blocks the port of the sample tube 21 to prevent the cell reagent in the sample tube 21 from overflowing during shaking or centrifugation. The plug 32 is engaged with the port of the sample tube 21. The outer edge 36 guides the port of the sample tube 21 to abut against the sealing ring 35 in the sealing groove 34, thereby sealing the port of the sample tube 21. The rubber pad 33 in the sliding groove 37... The plug 32 is pressed against the top, making it in close contact with the sample tube 21. The lower edge of the limiting groove 38 and the positioning rod 31 cooperate with the plug 32 to slide up and down stably, thereby achieving a stable seal at the port of the sample tube 21. The positioning plates 11 on both sides of the base plate 1 cooperate with the positioning grooves 41 on both sides of the cover plate 4 to facilitate the opening and closing of the cover plate 4 and the base plate 1. By pushing the locking block 22 on the clamping plate 2 with a finger, the locking block 22 separates from the locking groove 14, thereby allowing the clamping plate 2 to be easily moved out of the through groove 12, and thus allowing the clamping plate 2 and the sample tube 21 to be easily disassembled and cleaned.

[0021] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A 96-well plate for preventing cross-contamination, comprising a base plate (1), characterized in that: The bottom plate (1) is provided with a clamping plate (2) in its inner cavity, and a sample placement tube (21) is provided in the inner cavity of the clamping plate (2). The bottom plate (1) is provided with a cover plate (4) on its top, and a sealing mechanism (3) is provided on the top of the inner wall of the cover plate (4). The sealing mechanism (3) includes a positioning rod (31), which is fixedly connected to the top of the inner wall of the cover plate (4). A plug (32) is slidably connected to the outer wall of the positioning rod (31). A sealing groove (34) is provided at the bottom of the plug (32). A sealing ring (35) is provided in the sealing groove (34). The sealing ring (35) contacts the top of the placement tube (21). A rubber pad (33) is provided at the top of the plug (32). The rubber pad (33) is slidably connected to the outer wall of the positioning rod (31). An outer edge (36) is provided at the bottom of the plug (32). The outer edge (36) is inclined outward.

2. A 96-well plate for preventing cross-contamination according to claim 1, characterized in that: The base plate (1) has a side groove (15) on its outer side, and the cover plate (4) is inserted into the outside of the side groove (15). A positioning plate (11) is fixedly connected to one side of the base plate (1), and a positioning groove (41) is opened on one side of the cover plate (4). The positioning plate (11) and the positioning groove (41) are inserted into each other.

3. A 96-well plate for preventing cross-contamination according to claim 1, characterized in that: The bottom plate (1) has a through groove (12) in its inner cavity, and the clamping plate (2) is inserted into the through groove (12).

4. A 96-well plate for preventing cross-contamination according to claim 3, characterized in that: The bottom of the through groove (12) is provided with a circular groove (13), and one end of the laying tube (21) passes through the bottom of the clamp (2) and extends into the interior of the circular groove (13).

5. A 96-well plate for preventing cross-contamination according to claim 4, characterized in that: Both sides of the through groove (12) are provided with slots (14), and both sides of the clamp (2) are fixedly connected with blocks (22), which are inserted into the slots (14).

6. A 96-well plate for preventing cross-contamination according to claim 1, characterized in that: The outer wall of the positioning rod (31) is provided with a groove (37), and one end of the plug (32) is slidably connected to the inside of the groove (37).

7. A 96-well plate for preventing cross-contamination according to claim 1, characterized in that: The plug (32) has a limiting groove (38) inside, and the lower edge of the positioning rod (31) is slidably connected inside the limiting groove (38).

Images