Hybrid capture reaction constant temperature oscillation and sealing device

Abstract

The utility model belongs to molecular biology experiment equipment technical field, concretely relates to hybridization capture reaction constant temperature oscillation and sealing device, including the casing and the lid, the casing side wall is fixedly connected with the ring baffle near the top surface department, the lid is assembled in the casing top, the lid side wall is provided with locking assembly, the casing top with the lid between is provided with the sealing assembly, the casing inner chamber is provided with the support ring, the casing inner wall is fixedly connected with two limit rods, the limit rod penetrates the support ring and is connected with its movable joint, the support ring is detachably connected with the placing ring, the placing ring bottom is fixedly connected with the bracket, the placing ring inner wall is fixedly connected with a plurality of longitudinal and transverse cross spring, the support ring side wall is hinged with the drive link. The utility model can effectively ensure that the constant temperature oscillation area is in the highly sealed state, does not affect temperature loss at the same time, can also avoid the phenomenon that the impurity microorganism enters.

Description

Technical Field

[0001] This invention belongs to the field of molecular biology experimental equipment technology, specifically relating to a thermostatic oscillation and sealing device for hybridization capture reaction. Background Technology

[0002] In molecular biology experiments, the hybridization capture reaction is a crucial step that utilizes the specific binding of nucleic acid probes to target nucleic acid sequences to achieve the separation and detection of those sequences. The effectiveness of this reaction directly impacts the sensitivity and specificity of subsequent detection results. Current experimental procedures typically require hybridization capture reactions to meet three core requirements: a constant temperature environment, uniform oscillation, and sealed protection against contamination.

[0003] In existing equipment, the internal components are usually connected to the external environment during sample oscillation, resulting in poor sealing. This leads to large temperature fluctuations in the constant-temperature environment where the sample is oscillating, making it difficult to provide a constant-temperature oscillation environment for the sample. Furthermore, external impurities and microorganisms can easily enter the constant-temperature oscillation environment, affecting the oscillation reaction effect of the sample. Utility Model Content

[0004] The purpose of this invention is to provide a thermostatic oscillation and sealing device for hybridization capture reaction, which can effectively ensure that the thermostatic oscillation area is in a highly sealed state, without affecting temperature loss, and also prevent the entry of impurities and microorganisms.

[0005] The specific technical solution adopted by this utility model is as follows: A hybridization capture reaction isothermal oscillation and sealing device includes a housing and a cover. An annular baffle is fixedly connected to the side wall of the housing near the top surface. The cover is assembled on the top of the housing, and a locking component is provided on the side wall of the cover. A sealing assembly is provided between the top of the housing and the cover. A support ring is provided in the inner cavity of the housing. Two limiting rods are fixedly connected to the inner wall of the housing. The limiting rods pass through the support ring and are movably connected to it. A placement ring is detachably connected to the support ring, a bracket is fixedly connected to the bottom of the placement ring, a number of crisscrossing tension springs are fixedly connected to the inner wall of the placement ring, and a drive linkage is hinged to the side wall of the support ring.

[0006] Furthermore, the locking assembly includes a fixing plate fixedly connected to the four side walls of the cover. The side walls of the fixing plate are threaded with bolts, and one end of the bolt is rotatably connected to a top block located below the annular baffle. The top block abuts against the annular baffle.

[0007] Furthermore, the sealing assembly includes a sealing ring fixedly connected to the bottom surface of the cover. The sealing ring has a hollow structure. A sealing groove is provided on the top end face of the housing. The sealing ring is adapted to the sealing groove. Two connecting pipes are fixedly connected to the top surface of the cover. The connecting pipes communicate with the sealing ring.

[0008] Furthermore, each of the connecting pipes is equipped with a control valve; the control valve on the left is a check valve, and the control valve on the right is a pressure relief valve.

[0009] Furthermore, the top surface of the support ring is fixedly connected with multiple insertion rods, and the side wall of the placement ring is provided with multiple insertion holes, and the insertion rods are inserted into the insertion holes.

[0010] Furthermore, the bracket has a hollow structure.

[0011] The technical effects achieved by this utility model are as follows: The hybridization capture reaction isothermal oscillation and sealing device of this invention, through the cooperation between the housing, cover, locking assembly and sealing assembly, adopts a reciprocating oscillation method, which can hide the driving component that drives the oscillation inside the device, solving the problem of poor sealing caused by the traditional connecting rod needing to extend to the outside of the device. At the same time, with the locking assembly and sealing assembly, it can effectively ensure that the isothermal oscillation area is in a highly sealed state, without affecting the temperature loss, and also preventing the entry of impurities and microorganisms. Attached Figure Description

[0012] Figure 1 This is a perspective view of the present invention; Figure 2 This is a schematic diagram of the disassembled structure of this utility model; Figure 3 This is a partial cross-sectional structural schematic diagram of the present invention; Figure 4 This is a schematic diagram showing the disassembled structure of the support ring and the placement ring of this utility model; Figure 5 This is a bottom view of the bracket structure of this utility model; Figure 6 This is a utility model Figure 3 Enlarged view of point A in the image.

[0013] The attached diagram lists the components represented by each number as follows: 1. Housing; 2. Cover; 3. Annular baffle; 4. Fixing plate; 5. Bolt; 6. Top block; 7. Sealing groove; 8. Sealing ring; 9. Connecting pipe; 10. Control valve; 11. Support ring; 12. Limiting rod; 13. Placement ring; 14. Bracket; 15. Tension spring; 16. Drive linkage; 17. Insertion rod; 18. Insertion hole. Detailed Implementation

[0014] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0015] like Figures 1-6 As shown, the hybridization capture reaction isothermal oscillation and sealing device includes a housing 1 and a cover 2. An annular baffle 3 is fixedly connected to the side wall of the housing 1 near the top surface. The cover 2 is assembled on the top of the housing 1. A locking component is provided on the side wall of the cover 2. A heating tube is provided inside the housing 1. A sealing assembly is provided between the top of the housing 1 and the cover 2. A support ring 11 is provided in the inner cavity of the housing 1. Two limiting rods 12 are fixedly connected to the inner wall of the housing 1. The limiting rods 12 pass through the support ring 11 and are movably connected to it. A placement ring 13 is detachably connected to the support ring 11. A bracket 14 is fixedly connected to the bottom of the placement ring 13. Several crisscrossing tension springs 15 are fixedly connected to the inner wall of the placement ring 13. A drive linkage 16 is hinged to the side wall of the support ring 11. A control unit is located on one side of the housing 1. The control unit includes a touch screen, control buttons, and a cam drive mechanism. The cam drive mechanism drives the drive linkage 16 to reciprocate, thereby driving the support ring 11 to reciprocate and oscillate, achieving the oscillation of the sample container. This control and drive method is a mature existing technology and will not be detailed in this solution.

[0016] like Figure 3 and Figure 6 As shown, the locking assembly includes a fixing plate 4 fixedly connected to the four side walls of the cover 2. Bolts 5 are threadedly connected to the side walls of the fixing plate 4. One end of the bolt 5 is rotatably connected to a top block 6 located under the annular baffle 3. The top block 6 abuts against the annular baffle 3.

[0017] The locking components, located on all four sides of the cover 2, ensure even force distribution and secure fastening to the housing 1, thus achieving a seal on the housing 1. During constant-temperature oscillation, water can be injected into the housing 1, and the heating element can heat the water to a suitable temperature, maintaining a constant temperature inside the housing 1. Combined with the locking mechanism of the cover 2, this reduces heat loss.

[0018] like Figure 3 and Figure 6 As shown, the sealing assembly includes a sealing ring 8 fixedly connected to the bottom surface of the cover 2. The sealing ring 8 has a hollow structure. A sealing groove 7 is provided on the top end face of the housing 1. The sealing ring 8 is adapted to the sealing groove 7. Two connecting pipes 9 are fixedly connected to the top surface of the cover 2. The connecting pipes 9 communicate with the sealing ring 8.

[0019] Each of the connecting pipes 9 is equipped with a control valve 10. The control valve 10 on the left is a check valve, and the control valve 10 on the right is a pressure relief valve.

[0020] Specifically, after the housing 1 is attached to the housing 1, a syringe or external connector can be used to connect the connecting pipe 9 with the one-way valve on the side, so that gas can be injected into the sealing ring 8, causing the sealing ring 8 to expand and abut against the side wall of the sealing groove 7 for secondary sealing, thereby improving its sealing effect and preventing external impurities or microorganisms from seeping into the housing 1 and contaminating the sample.

[0021] like Figure 4 As shown, multiple insertion rods 17 are fixedly connected to the top surface of the support ring 11, and multiple insertion holes 18 are opened on the side wall of the placement ring 13, with the insertion rods 17 inserted into the insertion holes 18. By setting the docking method of the insertion rods 17 and the insertion holes 18, the support ring 11 and the placement ring 13 can be conveniently installed and disassembled. In use, the sample container can be fixed on the bracket 14 in advance, and then the placement ring 13 can be inserted into the support ring 11 for convenient use.

[0022] like Figure 5 As shown, the bracket 14 has a hollow structure. This facilitates contact between the sample container and the constant-temperature water inside the housing 1, and helps maintain the sample temperature during oscillation.

[0023] The working principle of this utility model is as follows: When it is necessary to shake the sample, the sample and container are first inserted between multiple tension springs 15 and abut against the bottom of the bracket 14 to support the container. At the same time, the elastic force between the tension springs 15 is used to squeeze and fix the container. Then, the placement ring 13 is placed on the support ring 11, and the insertion rod 17 is inserted into the insertion hole 18 to limit the placement ring 13. After that, the cover 2 is placed on the housing 1, and the bolts 5 in four directions are rotated to drive the top block 6 to move, so that the top block 6 moves to abut against the bottom of the annular baffle 3 to increase the sealing of the inside of the housing 1. Then, using a syringe or external high-pressure gas connection pipe 9, gas is injected into the sealing ring 8, causing the sealing ring 8 to expand and fill the sealing groove 7, further increasing the sealing performance. During oscillation, the drive component inside the housing 1 is activated to drive the drive linkage 16 to reciprocate. The drive linkage 16 drives the support ring 11 and the placement ring 13 to reciprocate along the limit rod 12, thereby performing constant temperature oscillation on the sample inside the container.

[0024] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A thermostatic oscillation and sealing device for hybridization capture reaction, characterized in that: Includes a housing (1) and a cover (2). A ring baffle (3) is fixedly connected to the side wall of the housing (1) near the top surface. The cover (2) is assembled on the top of the housing (1). A locking assembly is provided on the side wall of the cover (2). A sealing assembly is provided between the top of the housing (1) and the cover (2). A support ring (11) is provided in the inner cavity of the housing (1). Two limiting rods (12) are fixedly connected to the inner wall of the housing (1). The limiting rods (12) pass through the support ring (11) and are movably connected to it. A placement ring (13) is detachably connected to the support ring (11). A bracket (14) is fixedly connected to the bottom of the placement ring (13). Several intersecting tension springs (15) are fixedly connected to the inner wall of the placement ring (13). A drive linkage (16) is hinged to the side wall of the support ring (11).

2. The isothermal oscillation and sealing device for hybridization capture reaction according to claim 1, characterized in that: The locking assembly includes a fixing plate (4) fixedly connected to the four side walls of the cover (2). The side wall of the fixing plate (4) is threaded with a bolt (5). One end of the bolt (5) is rotatably connected to a top block (6) located under the annular baffle (3). The top block (6) abuts against the annular baffle (3).

3. The isothermal oscillation and sealing device for hybridization capture reaction according to claim 1, characterized in that: The sealing assembly includes a sealing ring (8) fixedly connected to the bottom surface of the cover (2). The sealing ring (8) is a hollow structure. A sealing groove (7) is provided on the top end face of the housing (1). The sealing ring (8) is adapted to the sealing groove (7). Two connecting pipes (9) are fixedly connected to the top surface of the cover (2). The connecting pipes (9) are connected to the sealing ring (8).

4. The isothermal oscillation and sealing device for hybridization capture reaction according to claim 3, characterized in that: Each of the connecting pipes (9) is equipped with a control valve (10). The control valve (10) on the left is a one-way valve, and the control valve (10) on the right is a pressure relief valve.

5. The isothermal oscillation and sealing device for hybridization capture reaction according to claim 1, characterized in that: The top surface of the support ring (11) is fixedly connected with multiple insert rods (17), and the side wall of the placement ring (13) is provided with multiple insertion holes (18), and the insert rods (17) are inserted into the insertion holes (18).

6. The isothermal oscillation and sealing device for hybridization capture reaction according to claim 1, characterized in that: The bracket (14) has a hollow structure.

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