A bacterial agent enrichment culture device

Abstract

The scheme provides a kind of bacterial agent enrichment culture device, comprising: main body, habitat simulation cavity is internally provided with, and habitat module assembly is in cavity;Gas inlet is provided on the main body and communicated with the cavity;Multiple independent liquid storage bins are provided in the main body, and each liquid storage bin is connected with injection pump communicated with the cavity;Jacket is wound outside the cavity, heating device is outside the jacket, and temperature control medium is in the jacket;Light source is set in the inner top of cavity;Collecting device is connected downstream of the cavity;Multiple temperature sensing switches and multiple light sensing switches, temperature sensing switch and light sensing switch are electrically connected in the control loop of corresponding injection pump.In the scheme, the environment of natural bacterial agent growth is simulated by habitat module assembly, the light and temperature are simulated by light source and jacket, the inducer and activator are added by injection pump, the function induction is carried out while ecological adaptation, the cultivation time of bacterial agent can be greatly reduced, and bacterial agent is easy to survive in real environment.

Description

Technical Field

[0001] This utility model relates to the field of microbial agent cultivation technology, and more specifically, to a microbial agent enrichment and cultivation device. Background Technology

[0002] Current enrichment methods primarily rely on static or shaking culture in a constant-temperature shaker using standard laboratory culture media. These methods suffer from several key drawbacks: First, ecological mismatch: standard culture media cannot replicate the nutritional limitations, spatial heterogeneity, interspecies interactions, and physicochemical gradients of the target bacteria in their natural environment. This results in enriched strains that, while proliferating in the laboratory, struggle to survive in real-world environments. Second, functional silencing: many functional genes (such as degradation enzyme genes and antibiotic synthesis genes) are in a "silent" state, requiring specific induction signals (such as substrates or quorum sensing molecules) for activation. Traditional enrichment methods lack precise induction mechanisms, leading to low enrichment efficiency. Therefore, a culture device is needed that can induce specific functions while maintaining an ecologically compatible natural environment. Utility Model Content

[0003] The present invention aims to provide a microbial agent enrichment and culture device to solve the problem that microbial agents cannot be cultured in a state close to the natural environment and at the same time induce the expression of specific genes.

[0004] To achieve the above objectives, according to one aspect of this utility model, a microbial agent enrichment and cultivation device is provided, comprising: a main body, within which a habitat simulation chamber is disposed, and within the habitat simulation chamber, a habitat module assembly is disposed; the main body is provided with multiple air inlets, all of which are connected to the habitat simulation chamber; multiple liquid storage chambers are disposed within the main body, the multiple liquid storage chambers are independent of each other, each liquid storage chamber is connected to an injection pump, and the injection port of each injection pump is connected to the habitat simulation chamber; a jacket is wrapped around the outside of the habitat simulation chamber, a temperature control medium is circulated inside the jacket, and a heating device is disposed outside the jacket; a light source is closable and disposed at the inner top of the habitat simulation chamber; a collection device is connected downstream of the habitat simulation chamber; multiple temperature-sensitive timer switches, each of which corresponds one-to-one with a multiple injection pump, and each temperature-sensitive timer switch is electrically connected to the control circuit of the corresponding injection pump; and multiple light-sensitive timer switches, each of which corresponds one-to-one with a multiple injection pump, and each light-sensitive timer switch is electrically connected to the control circuit of the corresponding injection pump.

[0005] Furthermore, the collection device includes: a collection chamber and an inlet, an outlet, a peristaltic pump, and a reflux tube disposed on the collection chamber, wherein the inlet is connected to the habitat simulation chamber, and the reflux tube is connected to the habitat simulation chamber.

[0006] Furthermore, the temperature-sensing timer switch includes a photoresistor, a 555 timer, and a first relay connected in sequence, wherein the first relay is connected in the control circuit of the injection pump; the light-sensing timer switch includes a light sensor, an LM393 comparator, a 555 timer, and a second relay connected in sequence, wherein the second relay is connected in the control circuit of the injection pump.

[0007] Furthermore, there are six liquid storage chambers, each containing a carbon source, a nitrogen source, signaling molecules, stress factors, a secretion simulation liquid, and a sensing activator.

[0008] Furthermore, the habitat module includes a pluggable packing support on which the substrate is mounted.

[0009] Furthermore, the injection pump is a micro-injection pump, and the outlet of the injection pump is located at the packing support.

[0010] Furthermore, a proportional valve is installed between the air inlet and the habitat simulation chamber.

[0011] In the technical solution of this utility model, the environment for the growth of natural fungal agents is simulated by the habitat module component, and the light and temperature are simulated by the light source and temperature control unit. After the temperature sensor and light sensor detect that the environment has been stable for a period of time, the control module controls the injection pump to add inducers and activators into the habitat simulation cavity. Functional induction is carried out while adapting to the ecology, which can significantly reduce the cultivation time of fungal agents, and the cultivated fungal agents are easy to survive in the real environment. Attached Figure Description

[0012] The accompanying drawings, which form part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an undue limitation of the present invention. In the drawings:

[0013] Figure 1 A schematic diagram of a microbial agent enrichment and culture device according to the present invention is shown. Detailed Implementation

[0014] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0015] See also Figure 1As shown in the figure, the bacterial agent enrichment and culture device of this scheme includes: a main body 10, a habitat simulation chamber 11 disposed within the main body 10, and habitat module components disposed within the habitat simulation chamber 11; multiple air inlets 12 disposed on the main body 10, all of which are connected to the habitat simulation chamber 11; multiple liquid storage tanks 14 disposed within the main body 10, the multiple liquid storage tanks 14 being independent of each other, each liquid storage tank 14 being connected to an injection pump 13, and the injection port of each injection pump 13 being connected to the habitat simulation chamber 11; and a jacket 15, which is wrapped around the outside of the habitat simulation chamber 11 to clamp... The jacket 15 contains a temperature-controlled medium circulating inside, and a heating device is installed outside the jacket 15; a light source 16, which can be turned on and off, is located at the top inside the habitat simulation chamber 11; a data acquisition device is connected downstream of the habitat simulation chamber 11; multiple temperature-sensing timer switches are connected one-to-one with multiple injection pumps 13, and each temperature-sensing timer switch is electrically connected to the control circuit of the corresponding injection pump 13; multiple light-sensing timer switches are also connected one-to-one with multiple injection pumps 13, and each light-sensing timer switch is connected to the control circuit of the corresponding injection pump 13.

[0016] As can be seen from the above description, the above embodiments of this utility model achieve the following technical effects: the habitat module component simulates the natural environment for the growth of fungal agents, the light source 16 and the jacket 15 simulate external light and temperature, and after the temperature timer switch and the light-sensing timer switch detect that the environment is stable, the corresponding injection pump 13 is controlled to add an inducer or activator into the habitat simulation cavity 11. Functional induction is carried out while adapting to the ecology, which can greatly reduce the cultivation time of the fungal agent, and the cultivated fungal agent is easy to survive in the real environment.

[0017] Preferably, the habitat module component includes a pluggable packing support 111 on which a substrate is mounted. The substrate can be one or more of the following: natural soil particles, sediment, plant root fragments, biochar, porous ceramic carrier, polyvinyl alcohol (PVA) gel beads, or artificial biofilm carrier. The substrate is used to simulate the physicochemical properties of the bacterial agent growth, and different environments can be simulated by plugging and unplugging different packing supports 111. The outlet of the injection pump 13 is preferably located at the top or middle of the packing support 111. A jacket 15 is also provided outside the habitat simulation chamber 11, and a temperature control medium is contained inside the jacket 15. The jacket 15, which surrounds the habitat simulation chamber 11, can change the growth temperature of the bacterial agent inside the habitat simulation chamber 11 by adjusting the temperature of the temperature control medium. The temperature control medium can be pure water, which has good thermal conductivity. The growth temperature of the bacterial agent is maintained between 15°C and 45°C through the circulation of the water medium in the jacket 15.

[0018] The temperature-sensing timer switch in this embodiment includes a photoresistor, a 555 timer, and a first relay connected in sequence, wherein the first relay is also electrically connected in the control circuit of the corresponding injection pump 13; the light-sensing timer switch includes a light sensor, an LM393 comparator, a 555 timer, and a second relay connected in sequence, wherein the second relay is also electrically connected in the control circuit of the corresponding injection pump 13. The photoresistor and the light sensor are disposed within the habitat simulation chamber.

[0019] For induction and activation, six reservoirs 14 can be installed within the main body 10. Each reservoir 14 contains a carbon source, a nitrogen source, a signaling molecule, a stress factor, a secretion simulation solution, and a sensing activator, respectively. The carbon source can be phenol, petroleum, or cellulose; the nitrogen source can be nitrate or urea; the signaling molecule can be AHL or Al-2; and the stress factor can be heavy metal ions. Each reservoir 14 is independently connected to a syringe pump 13. The syringe pump 13 is a micro-injection pump, with its outlet located above the support 111. The syringe pump 13 injects the liquid from the corresponding reservoir 14 into the habitat simulation chamber 11, completing the addition of the activator and inducing agent within the habitat simulation chamber 11. The injection volume of each syringe pump 13 can be controlled by adjusting the pump's operating time. To control the gas composition within the habitat simulation chamber 11, multiple air inlets 12 can be connected to different gas supply devices, preferably nitrogen, oxygen, and carbon dioxide supply devices. Proportional valves 18 are installed between each of the multiple air inlets 12 and the habitat simulation chamber 11 to precisely inject gas into the chamber. Different gas input ratios simulate anaerobic and oxygen-rich external environments and provide periodically alternating redox conditions. Simultaneously, the on / off state of the light source 16 simulates daytime and nighttime environments.

[0020] In this embodiment, each individual injection pump 13 is controlled by a corresponding temperature-sensitive timer switch and a light-sensitive timer switch. This overall setup ensures that the corresponding injection pump 13 will only be activated when the external environment simultaneously meets the requirements of light duration and temperature, injecting the substance in the corresponding storage tank 14 of the injection pump 13 into the habitat simulation chamber 11. This synergistic effect on the growth environment and induction timing of the bacterial agent improves the enrichment efficiency of the bacterial agent and shortens the cultivation cycle of the bacterial agent.

[0021] A collection device is also provided at the bottom of the main body 10. The collection device includes a collection box 17, an inlet, an outlet, a reflux pipe 192, and a peristaltic pump 191, all located on the box. The inlet is connected to the bottom of the habitat simulation chamber 11, and the outlet of the reflux pipe 192 is located at the lower middle end of the habitat simulation chamber 11. A peristaltic pump 191 is also provided before the inlet of the reflux pipe 192. Preferably, a retention unit is also provided downstream of the outlet. The retention unit can be a microfiltration membrane with a pore size of 0.22-0.45 μm to ensure the retention of the bacterial agent and the discharge of metabolic waste or unused substrate.

[0022] In this embodiment, the thermistor can be an NTC thermistor, the light sensor can be a BH1750 digital light sensor, and the LM393, 555 timer, and relay are all products that can be provided in the prior art.

[0023] Experimental results show that the microbial agent enrichment and culture device of this scheme shortens the microbial community construction cycle from 1-3 months of traditional methods to 2-3 weeks, and increases the microbial agent colonization success rate from the industry average of 30% to over 85%.

[0024] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A microbial agent enrichment and cultivation device, characterized in that, include: The main body (10) is provided with a habitat simulation cavity (11) and a habitat module component is provided in the habitat simulation cavity (11); the main body (10) is provided with multiple air inlets (12) and the multiple air inlets (12) are all connected to the habitat simulation cavity (11); Multiple liquid storage tanks (14) are disposed within the main body (10). The multiple liquid storage tanks (14) are independent of each other. Each liquid storage tank (14) is connected to an injection pump (13). The injection port of each injection pump (13) is connected to the habitat simulation cavity (11). A jacket (15) is arranged around the outside of the habitat simulation cavity (11). A temperature control medium circulates inside the jacket (15), and a heating device is provided outside the jacket (15). The light source (16) is openable and closable, and the light source (16) is located at the top inside the habitat simulation cavity (11); A collection device is connected downstream of the habitat simulation cavity (11); Multiple temperature-sensing timer switches are provided, and each of the multiple temperature-sensing timer switches corresponds one-to-one with a multiple injection pump (13). Each of the temperature-sensing timer switches is electrically connected to the control circuit of the corresponding injection pump (13). Multiple light-sensitive timer switches are provided, and each of the multiple light-sensitive timer switches corresponds one-to-one with a multiple injection pump (13). Each light-sensitive timer switch is electrically connected to the control circuit of the corresponding injection pump (13).

2. The microbial agent enrichment and cultivation device according to claim 1, characterized in that, The collection device includes: a collection box (17) and an inlet, an outlet, a peristaltic pump (191) and a reflux tube (192) disposed on the collection box (17), wherein the inlet is connected to the habitat simulation chamber (11) and the reflux tube (192) is connected to the habitat simulation chamber (11).

3. The microbial agent enrichment and cultivation device according to claim 1, characterized in that, The temperature-sensing timer switch includes a photoresistor, a 555 timer, and a first relay connected in sequence, wherein the first relay is connected in the control circuit of the injection pump (13); the light-sensing timer switch includes a light sensor, an LM393 comparator, a 555 timer, and a second relay connected in sequence, wherein the second relay is connected in the control circuit of the injection pump (13).

4. The microbial agent enrichment and cultivation device according to claim 1, characterized in that, There are 6 liquid storage chambers (14), and each of the 6 liquid storage chambers (14) contains: a carbon source, a nitrogen source, a signal molecule, a stress factor, a secretion simulation liquid and a sensing activator.

5. The microbial agent enrichment and cultivation device according to claim 1, characterized in that, The habitat module assembly includes a pluggable filler support (111) on which a substrate is loaded.

6. The microbial agent enrichment and cultivation device according to claim 5, characterized in that, The injection pump (13) is a micro-injection pump, and the outlet of the injection pump (13) is located at the packing support.

7. The microbial agent enrichment and cultivation device according to claim 1, characterized in that, A proportional valve (18) is provided between the air inlet (12) and the habitat simulation cavity (11).

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