Aerobic methane-oxidizing bacteria and anaerobic methane-oxidizing bacteria synchronous enrichment system

Abstract

The utility model provides a kind of aerobic methane-oxidizing bacteria and anaerobic methane-oxidizing bacteria synchronous enrichment system, belong to sewage treatment device technical field.System includes reaction device, hollow fiber membrane module, reflux bottle and control module, hollow fiber membrane module is located in reaction device;Reaction device is configured as enrichment MOB and DAMO bacterial flora;Reflux bottle, with reaction device communication, is configured as to the input solution to reaction device, and the solution after reaction in reflux reaction device;Reaction device is provided with air inlet, air inlet is configured as to hollow fiber membrane module with methane;Hollow fiber membrane module is configured as methane is evenly spread to solution, for MOB and DAMO bacterial flora to carry out metabolic reaction.Wherein, reflux bottle is also provided with dissolved oxygen meter and pH meter, and dissolved oxygen meter and pH meter are electrically connected control module.

Description

Technical Field

[0001] This utility model relates to the technical field of wastewater treatment devices, and in particular to a system for the simultaneous enrichment of aerobic and anaerobic methanogenic bacteria. Background Technology

[0002] In recent years, the application and research of anaerobic methanogenic bacteria in denitrification technology has seen a significant increase. This is mainly due to their unique advantages in greenhouse gas emission reduction and wastewater treatment. Anaerobic methanogenic bacteria can achieve the denitrification process using methane as an electron donor through synergistic action with denitrifying bacteria. This coupled reaction can simultaneously realize the resource utilization of methane and reduce the eutrophication impact of nitrogen oxides in water bodies.

[0003] Therefore, there is an urgent need for a reaction system that can utilize both anaerobic and aerobic methanogenic bacteria (MOB) for denitrification. Utility Model Content

[0004] To address the aforementioned problems in the existing technology, this utility model provides a simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria, thereby solving the problem of long enrichment cycles in existing simultaneous enrichment systems.

[0005] The specific details of the utility model are as follows: This invention proposes a simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria, comprising a reaction device, a hollow fiber membrane module, a reflux bottle, and a control module; the hollow fiber membrane module is disposed within the reaction device. The reaction apparatus is configured to enrich MOB and DAMO bacterial communities; The reflux bottle is connected to the reaction apparatus and is configured to input a solution into the reaction apparatus and to reflux the reacted solution in the reaction apparatus. The reaction device is provided with an air inlet, which is configured to introduce methane into the hollow fiber membrane module. The hollow fiber membrane module is configured to uniformly distribute the methane into the solution for the MOB and DAMO microbial communities to carry out metabolic reactions. The reflux bottle is also equipped with a dissolved oxygen meter and a pH meter. Both the dissolved oxygen meter and the pH meter are electrically connected to the control module. The dissolved oxygen meter is configured to collect the dissolved oxygen concentration data of the solution in the reflux bottle and transmit the concentration data to the control module. The pH meter is configured to collect the pH value of the solution in the reflux bottle and transmit the pH value to the control module. The control module is configured to receive the concentration data and control the dissolved oxygen concentration of the solution in the reflux bottle to a first concentration and a second concentration, wherein the first concentration is less than 0.1 mg / L and the second concentration is less than 1~2 mg / L, wherein the dissolved oxygen concentration of the solution in the reflux bottle is at the first concentration or the second concentration at the same time period. In addition, the pH value is received, and the pH value of the solution in the reflux bottle is maintained between 7.5 and 8 when the dissolved oxygen concentration of the solution in the reflux bottle is at the first concentration.

[0006] Optionally, the reflux bottle is equipped with an air pump, which is electrically connected to the control module; The control module is specifically configured to adjust the operating speed of the air pump when the dissolved oxygen concentration in the reflux bottle, which is characterized by the concentration data, is not at the first concentration or the second concentration, so that the dissolved oxygen concentration in the solution in the reflux bottle is at the first concentration or the second concentration.

[0007] Optionally, the reflux bottle is further provided with a pH adjustment module, which includes an adjustment pump, an adjustment valve, and a delivery pipe; The regulating pump and the regulating valve are both electrically connected to the control module, and both the regulating pump and the regulating valve are connected to the delivery pipe, which is adapted to introduce acid or alkali solution into the reflux bottle; Specifically, the control module is configured to control the opening and closing of the regulating pump and the regulating valve based on the pH data from the pH meter, thereby controlling the amount of acid or alkali solution added into the reflux bottle to adjust the pH.

[0008] Optionally, the reflux flask is further provided with a stirring device and a circulation pump, the circulation pump including a first circulation pump and a second circulation pump; the stirring device is located at the bottom of the reflux flask; the first circulation pump connects the reflux flask and the reaction apparatus, and the second circulation pump connects the reflux flask and the reaction apparatus; The control module is electrically connected to the stirring device, the first circulating pump, and the second circulating pump, and is configured to control the start and stop of the stirring device, the first circulating pump, and the second circulating pump. The stirring device is configured to stir the solution in the reflux bottle to prevent sludge from settling. The first circulation pump is configured to supply the solution to the reaction apparatus; The second circulation pump is configured to return the solution of the reaction apparatus to the reflux bottle.

[0009] Optionally, the reflux bottle is further provided with an air bag; The gas bag, connected to the return bottle, is configured to recover waste gas from the return bottle and to balance the pressure within the return bottle.

[0010] Optionally, the system further includes a methane transport device; The methane transfer device is connected to the air inlet to input methane into the inside of the hollow fiber membrane module; The reflux bottle is connected to the region outside the hollow fiber membrane assembly in the reaction device to introduce the solution into the outer region of the hollow fiber membrane assembly; The methane diffuses from the inside to the outside.

[0011] Optionally, the methane transfer device includes a methane cylinder configured to supply methane and a pressure reducing valve configured to control the methane flow rate; The methane cylinder is connected to the reaction apparatus via the pressure reducing valve.

[0012] Optionally, the reaction apparatus further includes a housing, on which a first liquid circulation port and a second liquid circulation port are provided; Wherein, the first liquid circulation interface is connected to the first circulation pump, and the second liquid circulation interface is connected to the second circulation pump; The first liquid circulation port and the second liquid circulation port are located on opposite sides of the length direction of the housing.

[0013] Optionally, the first liquid circulation port and the second liquid circulation port are located on opposite sides of the width direction of the housing; The length direction and the width direction are orthogonal.

[0014] Optionally, the reaction apparatus further includes a heating device and a temperature sensor, the heating device and the temperature sensor being electrically connected to the control module. The temperature sensor is configured to collect temperature data of the solution in the reaction apparatus and send the temperature data to the control module. The control module is specifically configured to receive the temperature data and send a heating signal to the heating device. The heating device is configured to heat the reaction device in response to the heating signal.

[0015] Compared with the prior art, the present invention has the following advantages: This invention provides a simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria, enabling the simultaneous enrichment of two different metabolic types of methanogenic bacteria (MOB and DAMO) within the same system. This improves the system's space utilization and operational efficiency. The hollow fiber membrane module 3 precisely controls the methane supply, enhancing mass transfer efficiency. It avoids disturbances caused by direct bubbling gas supply, helping to maintain a stable environment suitable for microbial attachment and growth. The hollow fiber membrane module 3 allows for precise control of the gas-liquid interface concentration by adjusting the methane flow rate as needed. The reflux bottle 4 and its monitoring devices (dissolved oxygen meter 41 and pH meter 42) monitor the dissolved oxygen and pH value in the culture medium in real time, facilitating timely adjustments to environmental conditions. This ensures that the microorganisms are in an optimal growth state during cultivation. It provides dynamic control support for the coexistence of MOB (aerobic) and DAMO (anaerobic). Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This diagram illustrates the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of the present invention. Figure 2 This diagram illustrates the connection relationship between the control modules of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment of the invention. Figure 3 A schematic diagram of the reaction device for the simultaneous enrichment system of aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown.

[0018] The accompanying figure is labeled as follows: 1. Reaction apparatus; 11. Air inlet; 12. Shell; 131. First liquid circulation interface; 132. Second liquid circulation interface; 2. Methane transfer device; 21. Methane cylinder; 22. Pressure reducing valve; 3. Hollow fiber membrane module; 4. Reflux bottle; 41. Dissolved oxygen meter; 42. pH meter; 43. Stirring device; 44. Regulating pump; 45. Regulating valve; 46. Delivery pipe; 51. First circulation pump; 52. Second circulation pump; 6. Gas bag; 7. Air pump; 8. Control module. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present utility model or its application or use. Based on the embodiments of the present utility model, any product identical or similar to the present utility model derived by anyone under the guidance of the present utility model or by combining the features of the present utility model with other prior art falls within the protection scope of the present utility model. Furthermore, all other embodiments obtained by those skilled in the art without inventive effort are within the protection scope of the present utility model.

[0020] Specific experimental steps or conditions are not specified in the embodiments; they can be performed according to the conventional experimental steps or conditions described in the prior art. Reagents and other instruments used, unless otherwise specified, are all commercially available conventional reagent products. Furthermore, the accompanying drawings are merely illustrative diagrams of embodiments of this utility model and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and therefore, repeated descriptions of them will be omitted. Some block diagrams shown in the drawings are functional entities and do not necessarily correspond to physically or logically independent entities.

[0021] Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of this utility model specification.

[0022] In the description of this utility model, it should be understood that the use of terms such as "first" and "second" to define the components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this utility model.

[0023] Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0024] Before providing a detailed description of the simultaneous enrichment system for aerobic and anaerobic methanotrophic bacteria provided by this invention, it is necessary to explain the relevant technologies as follows: Under the dual pressures of global climate change and ecological environmental protection, the development of novel low-carbon biological nitrogen removal technologies is of great significance. These technologies can not only efficiently remove nitrogen pollutants from wastewater and prevent eutrophication, but also significantly reduce the dependence on energy and external carbon sources in traditional nitrogen removal processes, achieving energy conservation and emission reduction. Furthermore, some low-carbon biological nitrogen removal technologies, such as those based on anaerobic methanogenic bacteria, can utilize methane, a greenhouse gas, as an energy source, converting pollutants into resources and balancing pollution control with greenhouse gas emission reduction goals. The development and application of these technologies align with the global trends of "carbon neutrality" and "sustainable development," providing crucial support for alleviating the ecological crisis, promoting a circular economy, and building a green society.

[0025] Among them, anaerobic and aerobic methanotrophic bacteria (AMB) biological nitrogen removal technologies have attracted widespread attention due to their dual advantages of resource utilization and low carbon emissions. This technology utilizes methane as an electron donor through anaerobic MAB bacteria to effectively remove nitrates and nitrites, while simultaneously achieving methane resource utilization, significantly reducing the energy consumption and external carbon source requirements of traditional nitrogen removal processes. However, the practical application of n-DAMO technology is still subject to some limitations, mainly because: First, the low metabolic rate of anaerobic ammonia oxidizing bacteria limits their widespread application in the treatment of high-flow-rate, high-nitrogen-load wastewater. Second, microbial enrichment is difficult; anaerobic ammonia oxidizing bacteria grow slowly and have stringent requirements for environmental conditions (such as temperature and pH), making the enrichment process time-consuming. Maintaining the dominant position of anaerobic ammonia oxidizing bacteria in mixed microbial communities remains a technical challenge. Third, their environmental adaptability is limited; anaerobic ammonia oxidizing bacteria are typically adapted to low-oxygen or anaerobic environments and find it difficult to adapt to high-oxygen, drastically fluctuating industrial wastewater environments. Fourth, reactor design and engineering challenges remain; existing reactor designs still need optimization to achieve efficient nitrogen removal and methane utilization, such as improving mass transfer efficiency and microbial immobilization effects.

[0026] In view of this, the present invention provides a simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria. Through this system, both aerobic and anaerobic methanogenic bacteria can be used for denitrification at the same time, and the aerobic and anaerobic methanogenic bacteria can be enriched and denitrified in stages in the reaction device, thereby making full use of the denitrification characteristics of aerobic methanogenic bacteria and anaerobic ammonium oxidizing bacteria, and achieving a balance between efficiency and economy.

[0027] To enable those skilled in the art to better understand this utility model, the following embodiments will be used to provide a detailed description of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria.

[0028] like Figure 1-3 As shown, Figure 1This diagram illustrates the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of the present invention. Figure 2 This diagram illustrates the connection relationship between the control modules of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment of the invention. Figure 3 This invention illustrates a schematic diagram of the reaction apparatus for a simultaneous enrichment system of aerobic and anaerobic methanogenic bacteria provided in an embodiment of the present invention. The present invention proposes a simultaneous enrichment system of aerobic and anaerobic methanogenic bacteria, comprising: Reaction apparatus, hollow fiber membrane module, reflux bottle and control module: The hollow fiber membrane module is located inside the reaction apparatus; The reaction apparatus is configured to enrich MOB and DAMO bacterial communities. The reflux bottle, connected to the reaction apparatus, is configured to input a solution into the reaction apparatus and to reflux the reacted solution back into the reaction apparatus. The reaction device is equipped with an air inlet, which is configured to introduce methane into the hollow fiber membrane module. The hollow fiber membrane module is configured to uniformly distribute methane into the solution for the MOB and DAMO microbial communities to carry out metabolic reactions. The reflux bottle is also equipped with a dissolved oxygen meter and a pH meter. Both the dissolved oxygen meter and the pH meter are electrically connected to the control module. The dissolved oxygen meter is configured to collect the dissolved oxygen concentration data of the solution in the reflux bottle and transmit the concentration data to the control module; the pH meter is configured to collect the pH value of the solution in the reflux bottle and transmit the pH value to the control module. The control module is configured to receive concentration data and control the dissolved oxygen concentration of the solution in the reflux bottle to a first concentration and a second concentration, wherein the first concentration is below 0.1 mg / L and the second concentration is below 1~2 mg / L, wherein the dissolved oxygen concentration of the solution in the reflux bottle is at either the first concentration or the second concentration at the same time period; and to receive pH value and maintain the pH value of the solution in the reflux bottle between 7.5 and 8 when the dissolved oxygen concentration of the solution in the reflux bottle is at the first concentration.

[0029] In this embodiment, the hollow fiber membrane assembly 3 is disposed inside the reaction device 1, with its two ends connected to the top and bottom of the reaction device 1, respectively.

[0030] The reflux bottle 4 is connected to the first liquid circulation interface 131 and the second liquid circulation interface 132 of the reaction device 1 via the first circulation pump 51 and the second circulation pump 52, respectively; the reaction device 1 is provided with an air inlet 11 connected to the methane transmission device 2, for supplying methane to the hollow fiber membrane module 3.

[0031] The reaction device 1 is a membrane bioreactor (MBfR) structure with an effective volume of about 700L. It adopts a rectangular or conical reactor design to optimize water flow distribution and mass transfer efficiency; or it introduces a multi-layer membrane module stacking structure to increase the biofilm attachment area.

[0032] Furthermore, the reflux bottle 4 is a tank with a stirrer and a volume of approximately 500L, used to reflux the solution, such as culture medium, in the reaction device 1; the reflux bottle 4 is equipped with two sets of pumps and a stirring device, used for reflux and input of solution respectively.

[0033] Furthermore, the methane transfer system 2 achieves bubble-free methane aeration through a superhydrophilic hollow fiber membrane module 3 with air circulation at both ends, and the methane partial pressure is adjustable (5-15 kPa), thereby improving the gas-liquid mass transfer efficiency.

[0034] Furthermore, the hollow fiber membrane module 3 is a tubular semi-permeable material with micron-sized pores. The inner side is a gas phase channel (methane flow), and the outer side is in contact with the liquid phase (reaction liquid flow). Through the diffusion principle, methane is transferred from the inner side of the membrane to the outer liquid phase for use by microorganisms.

[0035] This technical solution enables the simultaneous enrichment of two different metabolic types of methanogenic bacteria (MOB and DAMO) in the same system.

[0036] For example, when the dissolved oxygen concentration of the solution in the reflux bottle 4 is controlled at a first concentration, n-DAMO bacteria can be enriched. When the DO is gradually increased to a second concentration, MOB bacteria can be enriched, thus achieving stable operation of the bacterial community.

[0037] Specifically, this system enriches and acclimates MOB and DAMO bacterial communities through the control of methane supply, water quality, and dissolved oxygen concentration in the water, for use in denitrification wastewater treatment. Because MOB grows rapidly under aerobic conditions, the dissolved oxygen concentration in the initial reaction system is maintained at a very low level to enrich and acclimate DAMO. Once DAMO has accumulated to a certain level in the system, the dissolved oxygen concentration in the reactor is appropriately increased to enrich MOB.

[0038] This technical solution improves the system's space utilization and operational efficiency. The hollow fiber membrane module 3 precisely controls the methane supply, enhancing mass transfer efficiency. It avoids disturbances caused by direct bubbling gas supply, helping to maintain a stable environment suitable for microbial attachment and growth. The hollow fiber membrane module 3 allows for precise control of the gas-liquid interface concentration by adjusting the methane flow rate as needed. The reflux bottle 4, control module, and monitoring devices (dissolved oxygen meter 41 and pH meter 42) monitor dissolved oxygen and pH in the culture medium in real time, facilitating timely adjustments to environmental conditions. This ensures that microorganisms are in optimal growth conditions during cultivation. It provides dynamic control support for the coexistence of MOB (aerobic) and DAMO (anaerobic) microorganisms.

[0039] In some implementations... Figure 1 A schematic diagram of the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown, as follows: Figure 1 As shown, the reflux bottle 4 is equipped with a stirring device 43, a circulation pump, an air pump 7, a dissolved oxygen meter 41, and a pH meter 42. Both the dissolved oxygen meter 41 and the pH meter 42 are electrically connected to the control module. The dissolved oxygen meter 41 is configured to collect dissolved oxygen concentration data of the solution in the reflux bottle 4 and transmit the concentration data to the control module. The pH meter 42 is configured to collect the pH value of the solution in the reflux bottle 4 and transmit the pH value to the control module. The circulation pump includes a first circulation pump 51 and a second circulation pump 52. The stirring device 43 is located at the bottom of the reflux bottle 4. The first circulation pump 51 connects the reflux bottle 4 and the reaction device 1, and the second circulation pump 52 connects the reflux bottle 4 and the reaction device 1. The control module 8 is electrically connected to the stirring device 43, the first circulation pump 51, and the second circulation pump 52, and is configured to control the opening and closing of the stirring device 43, the first circulation pump 51, and the second circulation pump 52. The stirring device 43 is configured to stir the solution in the reflux bottle to prevent sludge from settling. The first circulation pump 51 is configured to input the solution into the reaction apparatus; The second circulation pump 52 is configured to return the solution of the reaction apparatus to the reflux bottle 4.

[0040] In this embodiment, the stirring device 43 can be configured as a stirring paddle or a spiral stirrer to prevent sludge sedimentation; the first circulation pump 51 is configured to input the solution into the reaction device 1; the second circulation pump 52 is configured to return the solution of the reaction device 1 to the reflux bottle 4. Furthermore, Figure 2This diagram illustrates the connection relationship between the control modules of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment of the invention; as shown. Figure 2 As shown, the reflux bottle 4 is equipped with an air pump 7, which is electrically connected to the control module 8; The control module 8 is specifically configured to adjust the operating speed of the air pump 7 when the dissolved oxygen concentration in the reflux bottle 4, which is characterized by the concentration data, is not at the first concentration or the second concentration, so that the dissolved oxygen concentration in the solution in the reflux bottle 4 is at the first concentration or the second concentration.

[0041] like Figure 2 As shown, the control module 8 adopts a PLC controller; the PLC controller is electrically connected to the dissolved oxygen meter 41, pH meter 42, air pump 7, regulating pump 44, regulating valve 45, stirring device 43, circulating pump, heating device, etc.; to realize automatic control of parameters such as dissolved oxygen concentration, pH value, temperature, liquid level, and pressure.

[0042] The control module 8 receives the concentration data and controls the dissolved oxygen concentration of the solution in the reflux bottle 4 to a first concentration and a second concentration, wherein the first concentration is less than 0.1 mg / L and the second concentration is less than 1~2 mg / L. During the same time period, the dissolved oxygen concentration of the solution in the reflux bottle 4 is at either the first concentration or the second concentration.

[0043] The control module 8 also receives the pH value and maintains the pH value of the solution in the reflux bottle 4 between 7.5 and 8 when the dissolved oxygen concentration of the solution in the reflux bottle 4 is at the first concentration.

[0044] The control module 8 is further specifically configured to adjust the operating speed of the air pump 7 when the concentration data indicates that the dissolved oxygen concentration of the solution in the reflux bottle 4 is not at the first concentration or the second concentration, so that the dissolved oxygen concentration in the reflux bottle 4 is at the first concentration or the second concentration.

[0045] In some embodiments, both the regulating pump 44 and the regulating valve 45 are electrically connected to the control module 8, and both are connected to the delivery pipe 46, which is adapted to introduce acid or alkali solution into the reflux bottle 4. Specifically, the control module 8 is configured to control the opening and closing of the regulating pump 44 and the regulating valve 45 based on the pH data from the pH meter 42, thereby controlling the amount of acid or alkali solution added into the reflux bottle 4 to adjust the pH. This ensures that, when the dissolved oxygen concentration in the solution within the reflux bottle 4 is at the first concentration, the pH value of the solution in the reflux bottle 4 is maintained between pH 7.5 and 8, so that DO is always below 0.1 mg / L, thus enriching the DAMO bacterial community.

[0046] For example, when the pH value is maintained above 7.5-8, acid is added; when it is in the range of 7.5-8 or below, alkali is added. This technical solution automatically adjusts the pH and dissolved oxygen concentration through air pump 7, pH adjustment module (adjusting pump 44, adjusting valve 45, delivery pipe 46), and control module 8, maintaining a suitable methane concentration and a suitable growth environment for microorganisms. This reduces pH fluctuations caused by the accumulation of metabolic products, improving bacterial activity and stability. It also enhances the system's automation level and ease of operation. Automatic feedback control of dissolved oxygen is achieved through air pump 7 and control module 8, avoiding errors from manual intervention. This ensures MOB growth while minimizing the inhibition of DAMO by oxygen. It also improves the system's intelligence and stability. The stirring device 43 and circulation pumps (first circulation pump 51, second circulation pump 52) prevent sludge deposition and improve the uniform distribution of nutrients and bacteria. The circulation pumps achieve liquid circulation, enhancing mass transfer efficiency and reaction uniformity. Finally, it improves the system's processing capacity and operational continuity.

[0047] In some implementations... Figure 1 A schematic diagram of the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown, as follows: Figure 1 As shown, the reflux bottle 4 is also provided with an air bag 6; Furthermore, the gas bag 6, connected to the reflux bottle 4, has a capacity of approximately 100L and is made of nylon / polyurethane composite material; it is configured to recover waste gas in the reflux bottle 4 and to balance the pressure inside the reflux bottle 4; the reflux bottle 4 is connected to the region outside the hollow fiber membrane assembly 3 in the reaction device 1 to input the solution into the outer region of the hollow fiber membrane assembly 3; wherein, the methane diffuses from the inside to the outside.

[0048] This technical solution utilizes a high-strength, well-sealed gas bag (6) to buffer the system during liquid circulation, preventing leakage or damage due to pressure changes. Maintaining stable internal system pressure contributes to smooth gas transmission and liquid circulation, and helps maintain stable methane concentration.

[0049] In some implementations... Figure 1 A schematic diagram of the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown, as follows: Figure 1 As shown, the methane transfer device 2 includes a methane cylinder 21 configured to supply methane and a pressure reducing valve 22 configured to control the methane flow rate; the methane cylinder 21 is connected to the reaction device 1 through the pressure reducing valve 22. Furthermore, the pressure reducing valve 22 has an input pressure of 200 bar and an output pressure of approximately 30 bar; the methane cylinder 21 is a steel cylinder with a working pressure of 137 bar or 200 bar.

[0050] This technical solution employs a pressure-reducing valve to regulate the pressure of gas from a high-pressure methane cylinder, ensuring that the methane pressure delivered to the reaction apparatus remains stable at approximately 30 bar. This control of the methane concentration helps maintain the gaseous environment required for microbial metabolism. The high-pressure methane cylinder (137 bar or 200 bar) used in conjunction with the pressure-reducing valve enables a continuous and stable low-pressure gas supply, reducing gas waste and improving the utilization efficiency of methane as a carbon and energy source. The pressure-reducing valve allows for flexible adjustment of the output pressure, making the system suitable for the cultivation needs of different types or stages of microorganisms, thus enhancing the system's versatility and adaptability.

[0051] In some implementations... Figure 3 A schematic diagram of the reaction device for the simultaneous enrichment system of aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown, as follows: Figure 3 As shown, the reaction apparatus 1 also includes a stainless steel housing 12. The housing 12 is provided with a first liquid circulation port 131 and a second liquid circulation port 132; wherein, the first liquid circulation port 131 is connected to the first circulation pump 51, and the second liquid circulation port 132 is connected to the second circulation pump 52; the first liquid circulation port 131 and the second liquid circulation port 132 are located on opposite sides of the housing 12 in the X direction.

[0052] The first liquid circulation interface 131 is connected to the first circulation pump 51, and the second liquid circulation interface 132 is connected to the second circulation pump 52.

[0053] like Figure 1As shown, the first liquid circulation port 131 and the second liquid circulation port 132 are located on opposite sides of the housing 12 in the width direction; wherein the length direction and the width direction are orthogonal, and the width direction is in... Figure 2 Specifically, it refers to both sides in the X direction.

[0054] In this embodiment, the first liquid circulation interface 131 and the second liquid circulation interface 132 can both be located on the outside of the hollow fiber membrane assembly 3.

[0055] Furthermore, the reaction apparatus 1 is also equipped with a heating device and a temperature sensor. The heating device and the temperature sensor are electrically connected to the control module 8. The temperature sensor is configured to collect temperature data of the solution in the reaction apparatus 1 and send the temperature data to the control module 8. The control module 8 is specifically configured to receive the temperature data and send a heating signal to the heating device. The heating device is configured to heat the reaction apparatus 1 in response to the heating signal.

[0056] In this embodiment, the shell 12 is a double-layer insulated shell, with the heating device and temperature sensor installed in the interlayer. The heating device and temperature sensor are electrically connected to the control module 8 to measure and heat the shell 12. The heating device is an electric heater.

[0057] The heating device can be an electric heating wire.

[0058] This technical solution, through the housing 12 and gas input interface 11, features a rational structural design that simplifies the gas transmission path and reduces the risk of leakage. It facilitates installation and maintenance, improving system integration and practicality. The heating device and temperature sensor address the crucial influence of temperature on microbial activity; automatic temperature control enhances bacterial growth efficiency. It ensures stable system operation within the set temperature range, adapting to the optimal growth conditions for different bacterial species. This enhances the system's environmental adaptability and reliability.

[0059] Example 1 like Figure 1 As shown, Figure 1 A schematic diagram of the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment is shown. This utility model provides a simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria, including a reaction device 1, a methane transfer device 2, a hollow fiber membrane module 3, and a reflux bottle 4. In this embodiment, the reaction device 1 includes a stainless steel shell 12; the reaction device 1 is equipped with a hollow fiber membrane module 3 inside, which divides the reaction space into an inner side (methane side) and an outer side (liquid side); the shell 12 is provided with a first liquid circulation interface 131 and a second liquid circulation interface 132, which are located on opposite sides in the width direction of the shell; the reaction device 1 is a membrane bioreactor (MBfR) structure with an effective volume of 700L, adopts a rectangular reactor design, introduces a multi-layer membrane module stacking structure to increase the biofilm attachment area, and the reaction device 1 is filled with a mixed activated sludge solution containing MOB and DAMO bacteria.

[0060] The reflux bottle 4 is a tank with a stirrer and a volume of approximately 500L, used to reflux the culture medium in the reaction device 1. The reflux bottle 4 is equipped with a dissolved oxygen meter 41, a pH meter 42, a pH adjustment module, an air bag 6, an air pump 7, a control module, a stirring device 43, and a circulation pump. The circulation pump includes a first circulation pump 51 and a second circulation pump 52. The reflux bottle 4 is connected to the reaction device 1 through a pipeline to realize liquid circulation.

[0061] The hollow membrane fiber component 3 is a tubular semi-permeable material with micron-sized pores. The inner side is a gas phase channel (methane flow), and the outer side is in contact with the liquid phase (reaction liquid flow). Through the diffusion principle, methane is transferred from the inner side of the membrane to the outer liquid phase for use by microorganisms.

[0062] In some examples, the hollow fiber membrane module 3 is made of hydrophobic polyethersulfone (PES); it has good gas permeability; and it is installed in the central region inside the reaction device 1.

[0063] In some embodiments, the system further includes a methane transport device 2; The methane transfer device 2 is connected to the air inlet 11 to input methane into the inside of the hollow fiber membrane assembly 3; The reflux bottle 4 is connected to the region outside the hollow fiber membrane assembly 3 in the reaction device 1, so as to input the solution into the outer region of the hollow fiber membrane assembly 3; The methane diffuses from the inside to the outside.

[0064] In this embodiment, the methane transmission device 2 introduces methane gas into the inside of the hollow fiber membrane assembly 3 through the air inlet 11.

[0065] In some examples, the methane transfer device 2 includes two sets of methane cylinders 21, one in operation and one on standby, and the methane cylinders 21 are connected to the reaction device 1 through the pressure reducing valve 22.

[0066] In this embodiment, Figure 2This diagram illustrates the connection relationship between the control modules of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in an embodiment of the present invention. Figure 2 As shown, the control module 8 adopts a PLC controller; it is electrically connected to the dissolved oxygen meter 41, pH meter 42, air pump 7, regulating pump 44, regulating valve 45, stirring device 43, circulating pump, heating device, etc.; and realizes automatic control of parameters such as dissolved oxygen concentration, pH value, temperature, liquid level, and pressure.

[0067] In this embodiment, as Figure 1 As shown, Figure 1 A schematic diagram of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment is shown; the stirring device 43 is located at the bottom of the reflux bottle 4; the first circulation pump 51 connects the reflux bottle 4 and the reaction device 1; the second circulation pump 52 connects the reflux bottle 4 and the reaction device 1.

[0068] In this embodiment, as Figure 1 As shown, Figure 1 A schematic diagram of the structure of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided in this embodiment is shown; the pH adjustment module includes an adjustment pump 44, an adjustment valve 45, and a delivery pipe 46; the adjustment pump 44 and the adjustment valve 45 are electrically connected to the control module, and the delivery pipe 46 is used to deliver the acid to the reflux bottle 4.

[0069] In this embodiment, as Figure 3 As shown, Figure 3 A schematic diagram of the reaction apparatus for the simultaneous enrichment system of aerobic and anaerobic methanogenic bacteria provided in an embodiment of this utility model is shown. The reaction apparatus 1 further includes a stainless steel shell 12, on which a liquid circulation interface 13 and a gas input interface 11 are provided. The gas input interface 11 is connected to the hollow fiber membrane module 3 and the methane cylinder 21. The liquid circulation interface 13 is connected to the reflux bottle 4 through the first circulation pump 51 and the second circulation pump 52.

[0070] In this embodiment, the heating device and temperature sensor are integrated into the interlayer of the housing of the reaction device 1; the control module maintains the reaction system at 30±1℃ based on the feedback data from the temperature sensor.

[0071] The operation process of the above-mentioned simultaneous enrichment system for aerobic and anaerobic methanotrophic bacteria is described below: (1) Start-up phase: Inject the initial culture medium (containing CH4 as a carbon source, NH4⁺ / NO3⁻ as a nitrogen source, trace elements, etc.) into the reflux bottle 4; inoculate with a mixed culture of MOB and DAMO. Turn on the second circulation pump 52 to allow the culture medium to enter the reaction device 1; Start the methane transmission device 2 and introduce methane into the inside of the hollow fiber membrane module 3; The initial dissolved oxygen concentration was set at 1~2 mg / L (aerobic environment) to promote MOB growth.

[0072] (2) Control module regulation strategy a. Dissolved oxygen control: The control module receives data collected by the dissolved oxygen meter 41 in real time. If the dissolved oxygen concentration is higher than 2 mg / L, reduce the speed of the air pump 7 or turn it off; If the dissolved oxygen concentration is below 1 mg / L, increase the speed of the air pump 7; When switching to the DAMO-dominated phase, the control module maintains the dissolved oxygen concentration at <0.1 mg / L (strict anaerobic conditions).

[0073] b. pH control: The control module receives the pH value fed back by the pH meter 42; When the dissolved oxygen concentration is low (<0.1 mg / L), the pH should be kept stable between 7.5 and 8. If the pH deviates from the set range, the control module starts the regulating pump 44 and the regulating valve 45 to add an appropriate amount of dilute sulfuric acid or NaOH solution to the reflux bottle 4 for adjustment.

[0074] c. Temperature control: The temperature sensor collects the temperature inside the reaction device 1; If the temperature is lower than the set value, the control module will activate the heating device to raise the temperature. If the temperature is too high, stop heating and allow heat to dissipate.

[0075] (3) Synergistic effect of microbial community: Methane transport path: Methane diffuses from the inside to the outside liquid environment through the hollow fiber membrane module 3; MOB metabolism: When the dissolved oxygen concentration is 1~2 mg / L, MOB uses oxygen to oxidize methane into CO2; DAMO metabolism: When the dissolved oxygen concentration drops to <0.1 mg / L, DAMO bacteria use nitrate as an electron acceptor to convert methane into CO2; Circulation and stirring: The stirring device 43 operates continuously to prevent sludge from settling; the first circulation pump 51 and the second circulation pump 52 ensure that the liquid flows evenly in the system.

[0076] (4) Waste gas treatment and pressure balance: Waste gases generated during the reaction (such as CO2 and unreacted CH4) are recovered through gas bag 6.

[0077] Under normal circumstances, reactor 1 typically operates in batch mode. Under sequencing batch operation conditions, the culture medium needs to be rationally configured based on the degradation rates of nitrate and nitrite. First, the degradation rates of nitrate and nitrite are monitored and their average values ​​are calculated to determine the daily amounts of nitrate and nitrite that DAMO and MOB can degrade in the reactor. Based on the principle of conservation between degradation and replenishment, the target concentration of the culture medium required for sequencing batch operation is calculated, and the culture medium is prepared according to the calculation results.

[0078] After the culture medium is prepared, the inlet and outlet flow rates of the reaction device 1 are controlled by the first circulation pump 51 and the second circulation pump 52, respectively. The first circulation pump 51 is used to add the prepared culture medium to the reactor at a set rate, while the second circulation pump 52 controls the liquid level and outflow rate in the reactor to ensure that the reactor completes the sequencing batch cycle under optimal operating conditions.

[0079] The preparation of the culture medium is described below as an example.

[0080] To ensure the normal growth of both anaerobic and aerobic methanogenic bacteria within the reactor, a culture medium needs to be prepared, requiring 80 mg / L of nitrite and 200 mg / L of nitrate. The nitrite will be provided by NaNO2 and KNO3, requiring 0.394 g / L of NaNO2 and 1.44 g / L of KNO3. The remaining formulation is as follows:

[0081]

[0082] The formula for trace element solution 1 is as follows:

[0083] The formula for trace element solution 2 is as follows:

[0084] After preparing the culture medium according to this formula, when replacing the culture medium in the reaction device (1), the new culture medium needs to be aerated to remove dissolved oxygen from the water. In the laboratory, N2 is used to aerate the culture medium for 15 minutes. After the aeration is completed, aeration needs to continue when replacing the culture medium in the reaction device (1) to prevent oxygen from entering. Based on the daily sampling and testing of the culture medium in the reactor, the culture medium needs to be replaced once a week, and when replacing the culture medium, the old culture medium needs to be completely drained.

[0085] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0086] For the sake of simplicity, the method embodiments are described as a series of actions. However, those skilled in the art should understand that the present invention is not limited to the described order of actions, as some steps can be performed in other orders or simultaneously according to the present invention. Furthermore, those skilled in the art should also understand that the embodiments described in the specification are preferred embodiments, and the actions and components involved are not necessarily essential to the present invention.

[0087] The above provides a detailed description of the simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria provided by this utility model. Specific examples have been used to illustrate the principle and implementation of this utility model. The description of the above embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation and application scope based on the idea of ​​this utility model. Therefore, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria, characterized in that, Includes a reaction device (1), a hollow fiber membrane module (3), a reflux bottle (4), and a control module (8): the hollow fiber membrane module (3) is located inside the reaction device (1); The reaction device (1) is configured to enrich MOB and DAMO bacterial communities; The reflux bottle (4) is connected to the reaction device (1) and is configured to input a solution into the reaction device (1) and reflux the solution after reaction in the reaction device (1); The reaction device (1) is provided with an air inlet, which is configured to introduce methane into the hollow fiber membrane module (3); The hollow fiber membrane module (3) is configured to uniformly distribute the methane into the solution for the MOB and DAMO microbial communities to carry out metabolic reactions; The reflux bottle (4) is also equipped with a dissolved oxygen meter (41) and a pH meter (42). Both the dissolved oxygen meter (41) and the pH meter (42) are electrically connected to the control module (8). The dissolved oxygen meter (41) is configured to collect the dissolved oxygen concentration data of the solution in the reflux bottle (4) and transmit the concentration data to the control module (8). The pH meter (42) is configured to collect the pH value of the solution in the reflux bottle (4) and transmit the pH value to the control module (8). The control module (8) is configured to receive the concentration data and control the dissolved oxygen concentration of the solution in the reflux bottle (4) to a first concentration and a second concentration, wherein the first concentration is less than 0.1 mg / L and the second concentration is less than 1~2 mg / L, wherein the dissolved oxygen concentration of the solution in the reflux bottle (4) is at the first concentration or the second concentration during the same time period. In addition, the pH value is received, and the pH value of the solution in the reflux bottle (4) is maintained between 7.5 and 8 when the dissolved oxygen concentration of the solution in the reflux bottle (4) is at the first concentration.

2. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The reflux bottle (4) is equipped with an air pump (7), which is electrically connected to the control module (8). The control module (8) is specifically configured to adjust the operating speed of the air pump (7) so that the dissolved oxygen concentration of the solution in the reflux bottle (4) is at the first concentration or the second concentration when the dissolved oxygen concentration represented by the concentration data is not at the first concentration or the second concentration.

3. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The reflux bottle (4) is also equipped with a pH adjustment module, which includes an adjustment pump (44), an adjustment valve (45), and a delivery pipe (46). The regulating pump (44) and the regulating valve (45) are both electrically connected to the control module (8), and the regulating pump (44) and the regulating valve (45) are both connected to the delivery pipe (46), which is adapted to pass acid or alkali solution into the reflux bottle (4); Specifically, the control module (8) is configured to control the opening and closing of the regulating pump (44) and the regulating valve (45) based on the pH data of the pH meter (42), thereby controlling the amount of acid or alkali solution added into the reflux bottle (4) to adjust the pH.

4. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The reflux bottle (4) is also equipped with a stirring device (43) and a circulation pump, the circulation pump including a first circulation pump (51) and a second circulation pump (52); the stirring device (43) is located at the bottom of the reflux bottle (4); the first circulation pump (51) connects the reflux bottle (4) and the reaction device (1), and the second circulation pump (52) connects the reflux bottle (4) and the reaction device (1). The control module (8) is electrically connected to the stirring device (43), the first circulation pump (51) and the second circulation pump (52), and is configured to control the opening and closing of the stirring device (43), the first circulation pump (51) and the second circulation pump (52); The stirring device (43) is configured to stir the solution in the reflux bottle to prevent sludge from settling. The first circulating pump (51) is configured to supply the solution to the reaction apparatus; The second circulation pump (52) is configured to return the solution of the reaction apparatus to the reflux bottle (4).

5. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 4, characterized in that, The reaction device (1) also includes a housing (12), on which a first liquid circulation port (131) and a second liquid circulation port (132) are provided. The first liquid circulation interface (131) is connected to the first circulation pump (51), and the second liquid circulation interface (132) is connected to the second circulation pump (52). The first liquid circulation port (131) and the second liquid circulation port (132) are located on opposite sides of the length direction of the housing (12).

6. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 5, characterized in that, The first liquid circulation port (131) and the second liquid circulation port (132) are located on opposite sides of the housing (12) in the width direction; The length direction and the width direction are orthogonal.

7. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The reflux bottle (4) is also provided with an air bag (6); The gas bag (6), connected to the return bottle (4), is configured to recover the waste gas in the return bottle (4) and balance the pressure inside the return bottle (4).

8. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The system also includes a methane transport device (2); The methane transfer device (2) is connected to the air inlet (11) to input methane into the inside of the hollow fiber membrane module (3); The reflux bottle (4) is connected to the region outside the hollow fiber membrane assembly (3) in the reaction device (1) to input the solution into the outer region of the hollow fiber membrane assembly (3); The methane diffuses from the inside of the hollow fiber membrane module (3) to the outside of the hollow fiber membrane module (3).

9. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 8, characterized in that, The methane transfer device (2) includes a methane cylinder (21) configured to supply methane and a pressure reducing valve (22) configured to control the methane flow rate. The methane cylinder (21) is connected to the reaction device (1) via the pressure reducing valve (22).

10. The simultaneous enrichment system for aerobic and anaerobic methanogenic bacteria according to claim 1, characterized in that, The reaction device (1) is also equipped with a heating device and a temperature sensor, which are electrically connected to the control module (8). The temperature sensor is configured to collect temperature data of the solution in the reaction device (1) and send the temperature data to the control module (8). The control module (8) is specifically configured to receive the temperature data and send a heating signal to the heating device. The heating device is configured to heat the reaction device (1) in response to the heating signal.

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