Exosome filtration device

Abstract

The utility model discloses an exosome filtering device belongs to the technical field of filtering equipment, the utility model discloses an exosome filtering device, and the limiting cooperation part of filter core is located at the outer circumferential side of filter core, when the upper casing and lower casing fixed connection, make upper limiting portion and lower limiting portion clamping limiting cooperation part, to with filter core fixed in the filter cavity, can make exosome filtering device form integral type structure, not only is favorable to reducing the volume of exosome filtering device, and can simplify the structure, reduce the cost, can be used for small amount or domestic extraction exosome, and the commonality is stronger.

Description

Technical Field

[0001] This utility model relates to the field of filtration equipment technology, and in particular to an exosome filtration device. Background Technology

[0002] Exosomes are tiny membrane vesicles secreted by cells, widely distributed in various biological fluids. They range in diameter from approximately 30 to 200 nanometers and exhibit a cup-shaped or spherical structure. Enclosed by a phospholipid bilayer, exosomes are rich in cholesterol, sphingomyelin, ceramides, and short-chain or long-chain saturated fatty acids. In addition, exosomes contain cell-specific proteins, lipids, and nucleic acids, including mRNA, non-coding RNA, and DNA. They carry the cell-specific proteins, lipids, and nucleic acids, acting as mediators of intercellular signaling and substance delivery.

[0003] In related technologies, the use of exosomes can improve skin firmness and enhance its resistance to photoaging. However, existing exosome filtration devices are typically bulky, complex in structure, and expensive, making them suitable only for industrial production and not for home skincare. Utility Model Content

[0004] The purpose of this invention is to provide an exosome filtration device that is small in size, simple in structure, low in cost, and more versatile.

[0005] To achieve the above objectives, the following technical solution is provided:

[0006] Exosome filtration device, comprising:

[0007] A filter housing includes an upper housing and a lower housing, the upper housing and the lower housing being fixedly connected to form a filter chamber; the upper housing is provided with an upper limit part and a feed inlet, and the lower housing is provided with a lower limit part and a discharge outlet;

[0008] The filter element has a limiting fitting part on its outer periphery. When the upper housing and the lower housing are fixedly connected, the upper limiting part and the lower limiting part can clamp the limiting fitting part to fix the filter element in the filter chamber. The filter element divides the filter chamber into a feeding chamber and a discharging chamber. The feeding port is connected to the feeding chamber, and the discharging port is connected to the discharging chamber.

[0009] As a preferred embodiment of the above-mentioned exosome filtration device, the bottom of the discharge chamber is provided with a support portion, which can abut against the bottom of the filter element.

[0010] As a preferred embodiment of the above-mentioned exosome filtration device, the support portion includes a plurality of protrusions arranged on the bottom wall of the discharge chamber;

[0011] A flow channel is formed between two adjacent protrusions, and one end of the flow channel is connected to the discharge port.

[0012] As a preferred embodiment of the above-mentioned exosome filtration device, the protrusion includes a proximal end and a distal end that are disposed opposite to each other, wherein the proximal end is closer to the discharge port than the distal end.

[0013] The width D of the flow channel gradually decreases from the distal end to the proximal end.

[0014] As a preferred embodiment of the above-mentioned exosome filtration device, the limiting mating part includes a first mating support; the filter element includes a filter paperboard, and the first mating support is the edge of the filter paperboard.

[0015] As a preferred embodiment of the above-mentioned exosome filtration device, the limiting fitting part further includes a second fitting support; the filter element further includes a filter screen, which is disposed on the upper side of the filter paperboard, and the second fitting support is the edge of the filter screen;

[0016] And / or, the support portion includes a plurality of protrusions arranged on the bottom wall of the discharge chamber; the filter element also includes a filter membrane disposed on the underside of the filter paperboard, and the filter membrane is placed on the plurality of protrusions.

[0017] As a preferred technical solution of the above-mentioned exosome filtration device, the lower limiting part is a first step provided in the lower housing, the first step has a ring structure, and the limiting fitting part is placed on the first step;

[0018] And / or, the lower housing is provided with a second step, the second step is in the form of a ring, the second step is offset from the upper limit part, and the edge of the filter membrane is placed on the second step.

[0019] As a preferred technical solution of the above-mentioned exosome filtration device, the feed inlet is provided with a feed connector;

[0020] And / or, the discharge port is provided with a discharge connector.

[0021] As a preferred embodiment of the above-mentioned exosome filtration device, the inner diameter of the inlet is equal to the inner diameter of the feed chamber;

[0022] And / or, the inner diameter of the discharge port is smaller than the inner diameter of the discharge compartment.

[0023] As a preferred technical solution of the above-mentioned exosome filtration device, the filter housing has a cylindrical structure.

[0024] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0025] The exosome filtration device of this utility model has a limiting fitting part of the filter element located on the outer periphery of the filter element. When the upper shell and the lower shell are fixedly connected, the upper limiting part and the lower limiting part clamp the limiting fitting part to fix the filter element in the filtration chamber. This allows the exosome filtration device to form an integrated structure, which not only helps to reduce the size of the exosome filtration device, but also simplifies the structure and reduces costs. It can be used for small-scale or household exosome extraction and has greater versatility. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the exosome filtration device in an embodiment of the present invention;

[0027] Figure 2 This is a schematic diagram of the upper shell structure in an embodiment of the present utility model;

[0028] Figure 3 This is a schematic diagram of the lower shell structure in an embodiment of the present invention;

[0029] Figure 4 This is a cross-sectional view of the lower shell in an embodiment of the present invention;

[0030] Figure 5 This is a schematic diagram of the lower housing structure when the discharge connector is a pagoda connector in an embodiment of this utility model;

[0031] Figure 6 This is a schematic diagram of the lower housing structure when the discharge connector is a Luer connector in an embodiment of this utility model.

[0032] Figure label:

[0033] 1. Filter housing; 11. Upper housing; 111. Upper limit position; 112. Feed inlet; 12. Lower housing; 121. Lower limit position; 122. Discharge outlet; 1221. Discharge connector; 123. Second step; 13. Filter chamber; 131. Feed chamber; 132. Discharge chamber; 1321. Protrusion; 13211. Proximal end; 13212. Distal end; 1322. Flow guide channel; 2. Filter element. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0035] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0036] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0037] In the description of this utility model, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are used only for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0038] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0039] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0040] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0041] like Figures 1 to 6 As shown, this embodiment provides an exosome filtration device, including a filter housing 1 and a filter element 2. The filter housing 1 includes an upper housing 11 and a lower housing 12, which are fixedly connected to form a filter chamber 13. The upper housing 11 is provided with an upper limit part 111 and a feed inlet 112, and the lower housing 12 is provided with a lower limit part 121 and a discharge outlet 122. The outer periphery of the filter element 2 is provided with a limiting fitting part. When the upper housing 11 and the lower housing 12 are fixedly connected, the upper limit part 111 and the lower limit part 121 can clamp the limiting fitting part to fix the filter element 2 in the filter chamber 13. The filter element 2 divides the filter chamber 13 into a feed compartment 131 and a discharge compartment 132. The feed inlet 112 is connected to the feed compartment 131, and the discharge outlet 122 is connected to the discharge compartment 132.

[0042] In this embodiment of the exosome filtration device, the limiting fitting part of the filter element 2 is located on the outer periphery of the filter element 2. When the upper shell 11 and the lower shell 12 are fixedly connected, the upper limiting part 111 and the lower limiting part 121 clamp the limiting fitting part to fix the filter element 2 in the filter chamber 13. This allows the exosome filtration device to form an integrated structure, which not only helps to reduce the size of the exosome filtration device, but also simplifies the structure and reduces costs. It can be used for small-scale or household exosome extraction and has greater versatility.

[0043] Optionally, the filter housing 1 has a cylindrical structure. Furthermore, the filter chamber 13 also has a cylindrical structure, which helps to simplify the structure of the filter housing 1, facilitates the manufacture and assembly of the filter housing 1, and reduces costs, making the exosome filtration device more suitable for small-volume or home-use exosome extraction.

[0044] Specifically, the inlet 112 and the outlet 122 are located at both ends of the cylindrical filter chamber 13 along its axial direction, and the filter element 2 has an inlet surface facing the inlet 112 and an outlet surface facing the outlet 122.

[0045] Optionally, the upper housing 11 and the lower housing 12 are fixed by threads or hot-melt welding, which can improve the ease of assembly.

[0046] Optionally, the bottom of the discharge chamber 132 is provided with a support part, which can abut against the bottom of the filter element 2, thereby supporting the filter element 2, improving the installation stability and reliability of the filter element 2, and helping to ensure filtration accuracy.

[0047] Optionally, the support includes multiple protrusions 1321 arranged on the bottom wall of the discharge chamber 132. That is, the multiple protrusions 1321 are spaced apart, which can improve the support effect on the filter element 2 without affecting the flow of material in the discharge chamber 132 to the discharge port 122, which is conducive to further simplifying the structure of the exosome filtration device.

[0048] Optionally, a flow guiding channel 1322 is formed between two adjacent protrusions 1321. One end of the flow guiding channel 1322 is connected to the discharge port 122, thereby guiding the filtered material through the protrusions 1321 and promoting the rapid discharge of the material through the discharge port 122 to improve filtration efficiency. In other words, the protrusions 1321 integrate the functions of support and flow guiding.

[0049] In this embodiment, the discharge port 122 is located at the bottom of the discharge chamber 132, which facilitates the complete and rapid discharge of the filtered material through the discharge port 122 into the discharge chamber 132. Exemplarily, the axis of the discharge port 122 coincides with the axis of the cylindrical filter chamber 13, that is, the discharge port 122 is located at the center of the discharge chamber 132.

[0050] Specifically, the protrusion 1321 is an elongated structure extending radially along the discharge port 122, which improves the flow guiding effect of the guide channel 1322. Furthermore, multiple protrusions 1321 are evenly distributed circumferentially along the discharge port 122, which improves the flow guiding consistency of multiple guide channels 1322, avoids local accumulation of material in the discharge chamber 132, and helps to improve filtration efficiency.

[0051] Optionally, the protrusion 1321 includes a proximal end 13211 and a distal end 13212 disposed opposite to each other, with the proximal end 13211 being closer to the discharge port 122 than the distal end 13212; from the distal end 13212 toward the proximal end 13211, the width D of the flow channel 1322 gradually decreases, thereby promoting the convergence of the filtered material toward the discharge port 122, which is beneficial to improving the filtration efficiency.

[0052] Optionally, a first gap L is provided between the proximal end 13211 and the discharge port 122. That is, the proximal end 13211 of the protrusion 1321 does not extend to the discharge port 122, so that the material discharged from each guide channel 1322 can first converge before entering the discharge port 122, which helps to increase the speed at which the material is discharged from the filter housing 1, thereby improving the filtration efficiency. It should be noted that the first gap is a known value and can be determined based on repeated experiments or experience, and is not limited here.

[0053] Optionally, the limiting fitting part includes a first fitting support; the filter element 2 includes a filter paperboard, and the first fitting support is the edge of the filter paperboard. That is, when the upper housing 11 and the lower housing 12 are fixedly connected, the first fitting support is clamped by the upper limiting part 111 and the lower limiting part 121 to fix the filter paperboard. Since the filter paperboard has a certain thickness and rigidity, the edge of the filter paperboard can be used as the first fitting support, which helps to simplify the structure and processing technology of the filter element 2 and reduce costs.

[0054] Furthermore, the limiting and fitting part also includes a second fitting support; the filter element 2 also includes a filter screen, which is disposed on the upper side of the filter paperboard, and the second fitting support is the edge of the filter screen. When the upper housing 11 and the lower housing 12 are fixedly connected, the first fitting support and the second fitting support are clamped simultaneously by the upper limiting part 111 and the lower limiting part 121 to fix the filter paperboard and the filter screen. Since the filter screen has a certain degree of toughness, and the filter paperboard provides a certain support for the filter screen, the filter screen can withstand a certain pressure. Therefore, the edge of the filter screen can be used as the second fitting support, which is beneficial to improving the installation stability, installation convenience and reliability of the filter element 2. For example, the filter screen is a nylon mesh.

[0055] Furthermore, the filter element 2 also includes a filter membrane, which is disposed on the underside of the filter paperboard and placed on multiple protrusions 1321. That is, the filter membrane is laid on the lower surface of the filter paperboard and supported and positioned by the multiple protrusions 1321. Furthermore, in order to improve the stability of the filter membrane, the multiple protrusions 1321 press the filter membrane firmly against the lower surface of the filter paperboard.

[0056] Specifically, the filter screen, filter paperboard, and filter membrane are stacked sequentially from top to bottom along the axial direction of the cylindrical structure. This simplifies the structure of the exosome filtration device, reduces costs, and facilitates assembly.

[0057] It should be noted that the filter paperboard has irregular filter holes, and the minimum pore size of the filter hole is the particle size of the material that can pass through the filter hole.

[0058] In this embodiment, the minimum pore size of the filter paperboard is smaller than the pore size of the filter screen. The pore size of the filter membrane is smaller than the minimum pore size of the filter paperboard, thereby achieving multi-stage filtration and improving filtration effect and efficiency.

[0059] For example, the mesh size of the filter screen ranges from 100 mesh to 300 mesh; in other words, the pore size of the filter screen ranges from 50 μm to 150 μm. That is, the pore size of the filter screen can be any value between 50 μm and 150 μm, such as 50 μm, 55 μm, 60 μm, 65 μm, 70 μm, 75 μm, 80 μm, 85 μm, 90 μm, 95 μm, 100 μm, 105 μm, 110 μm, 115 μm, 120 μm, 125 μm, 130 μm, 135 μm, 140 μm, 145 μm, or 150 μm.

[0060] For example, the pore size of the filter paperboard ranges from 0.1μm to 4μm. That is, the pore size of the filter paperboard can be any value between 0.1μm and 4μm, such as 0.1μm, 0.5μm, 1μm, 1.5μm, 2μm, 2.5μm, 3μm, 3.5μm, or 4μm.

[0061] For example, the thickness of the filter paperboard is 4mm ± 0.02mm, which can reduce the space occupied by the filter paperboard while meeting the strength requirements of the filter paperboard, so as to ensure the reliability of the filter element 2.

[0062] For example, the pore size of the filter membrane is 0.22 μm. It should be noted that the 0.22 μm filter membrane can also intercept bacteria, thereby achieving sterile filtration.

[0063] Optionally, the lower limiting part 121 is a first step provided inside the lower housing 12. The first step has an annular structure, and the limiting mating part is placed on the first step. It should be noted that, for the limiting mating part formed by the edge of the filter paper and the edge of the filter screen, the annular structure of the first step can increase the contact area between the limiting mating part and the lower limiting part 121, thereby improving the installation stability and reliability of the filter element 2.

[0064] In this embodiment, the upper housing 11 and the lower housing 12 are fixedly connected by threads. Specifically, the upper housing 11 is provided with external threads, and the lower housing 12 is provided with internal threads. The lower end face of the upper housing 11 is the upper limit part 111.

[0065] Optionally, the lower housing 12 is provided with a second step 123, which is annular in structure and offset from the upper limit portion 111. The edge of the filter membrane is placed on the second step 123. That is, when the upper housing 11 and the lower housing 12 are fixedly connected, the upper limit portion 111 will not contact the second step 123, and the filter membrane will not be squeezed, thus protecting the filter membrane and preventing problems such as filter membrane damage due to pressure. The second step 123 with its annular structure supports the edge of the filter membrane, which can improve the installation stability and reliability of the filter membrane.

[0066] Specifically, the top surface of the protrusion 1321 is flush with the top surface of the second step 123, or the top surface of the protrusion 1321 is slightly lower than the top surface of the second step 123.

[0067] Furthermore, the inner diameter of the filter chamber 13 at the location of the first step is larger than the inner diameter of the filter chamber 13 at the location of the second step 123, and the second step 123 is located below the first step. This prevents the filter membrane from being squeezed and allows the filter membrane to adhere tightly to the filter paperboard, resulting in better filtration. Specifically, the outer diameter of the filter membrane is equal to the inner diameter of the filter chamber 13 at the location of the filter membrane, thereby ensuring filtration efficiency. For example, the outer diameter of the filter membrane is 47 mm.

[0068] Optionally, the inner diameter of the feed inlet 112 is equal to the inner diameter of the feed chamber 131. That is, the end of the feed chamber 131 facing away from the filter element 2 is open, and this open end is the feed inlet 112. In other words, by connecting to an upstream exosome extraction device (such as a blender) through this open end, the inner diameter of the feed inlet 112 can be increased, improving feeding convenience. Alternatively, the feed inlet 112 can also be connected to a container holding filtered material, facilitating exosome extraction for home use.

[0069] Of course, the inner diameter of the inlet 112 can also be smaller than the inner diameter of the feed chamber 131. Furthermore, the inlet 112 is provided with a feed connector so that the inlet 112 can be connected to the upstream exosome extraction equipment through the feed connector.

[0070] Optionally, the inner diameter of the discharge port 122 is smaller than the inner diameter of the discharge chamber 132, so that the filtered material can be gathered in the discharge chamber 132 and then discharged through the discharge port 122, which facilitates the collection of the filtered material.

[0071] Optionally, the discharge port 122 is provided with a discharge connector 1221 to quickly connect the discharge port 122 to a downstream exosome extraction instrument (such as a vacuum pump, peristaltic pump, etc.). For example, the discharge connector 1221 is a pagoda connector or a Luer connector to facilitate compatibility with commercially available exosome extraction instruments.

[0072] For example, the method of using the exosome filtering device in this embodiment is as follows:

[0073] The feed inlet 112 is connected to the upstream exosome extraction equipment so that the material to be filtered is injected into the feed chamber 131 through the feed inlet 112.

[0074] The discharge port 122 is connected to a vacuum pump to generate negative pressure, which promotes the material in the feed chamber 131 to pass through the filter element 2 for filtration, thereby improving the filtration efficiency.

[0075] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention. The scope of the present invention is determined by the scope of the appended claims.

Claims

1. An exosome filtration device, characterized in that, include: A filter housing (1) includes an upper housing (11) and a lower housing (12), wherein the upper housing (11) and the lower housing (12) are fixedly connected to form a filter chamber (13); the upper housing (11) is provided with an upper limit part (111) and a feed inlet (112), and the lower housing (12) is provided with a lower limit part (121) and a discharge outlet (122); The filter element (2) has a limiting fitting part on its outer periphery. When the upper housing (11) and the lower housing (12) are fixedly connected, the upper limiting part (111) and the lower limiting part (121) can clamp the limiting fitting part to fix the filter element (2) in the filter chamber (13). The filter element (2) divides the filter chamber (13) into a feeding chamber (131) and a discharging chamber (132). The feeding port (112) is connected to the feeding chamber (131), and the discharging port (122) is connected to the discharging chamber (132). The bottom of the discharging chamber (132) is provided with a support part, which can abut against the bottom of the filter element (2).

2. The exosome filtration device according to claim 1, characterized in that, The support includes a plurality of protrusions (1321) arranged on the bottom wall of the discharge chamber (132); A flow channel (1322) is formed between two adjacent protrusions (1321), and one end of the flow channel (1322) is connected to the discharge port (122).

3. The exosome filtration device according to claim 2, characterized in that, The protrusion (1321) includes a proximal end (13211) and a distal end (13212) disposed opposite to each other, wherein the proximal end (13211) is closer to the discharge port (122) than the distal end (13212); The width D of the flow channel (1322) gradually decreases from the distal end (13212) to the proximal end (13211).

4. The exosome filtration device according to claim 1, characterized in that, The limiting fitting part includes a first fitting support; the filter element (2) includes a filter paperboard, and the first fitting support is the edge of the filter paperboard.

5. The exosome filtration device according to claim 4, characterized in that, The limiting fitting part also includes a second fitting support; the filter element (2) also includes a filter screen, which is disposed on the upper side of the filter paperboard, and the second fitting support is the edge of the filter screen; And / or, the support includes a plurality of protrusions (1321) arranged on the bottom wall of the discharge chamber (132); the filter element (2) also includes a filter membrane, the filter membrane being disposed on the underside of the filter paperboard, the filter membrane being placed on the plurality of protrusions (1321).

6. The exosome filtration device according to claim 5, characterized in that, The lower limiting part (121) is a first step provided in the lower housing (12), the first step has a ring structure, and the limiting fitting part is placed on the first step; And / or, the lower housing (12) is provided with a second step (123), the second step (123) is in the form of an annular structure, the second step (123) is offset from the upper limit part (111), and the edge of the filter membrane is placed on the second step (123).

7. The exosome filtration device according to claim 1, characterized in that, The feed inlet (112) is equipped with a feed connector; And / or, the discharge port (122) is provided with a discharge connector (1221).

8. The exosome filtration device according to claim 1, characterized in that, The inner diameter of the feed inlet (112) is equal to the inner diameter of the feed compartment (131); And / or, the inner diameter of the discharge port (122) is smaller than the inner diameter of the discharge compartment (132).

9. The exosome filtration device according to claim 1, characterized in that, The filter housing (1) has a cylindrical structure.

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