Nucleic acid collection tube and nucleic acid detection device

Abstract

A nucleic acid collection tube comprises a test tube, a convex ring, a tube cover, a buckle, a convex ring is arranged near the upper end ring wall of the test tube, the convex ring is provided with a clamping groove, the clamping groove is trapezoidal, one side of the tube cover is hinged with the test tube, the lower end surface of the tube cover and the upper end surface of the test tube are both smooth planes, the upper end of the buckle is hinged with the other side of the tube cover, the lower end of the buckle is matched with the shape of the clamping groove, the side wall of the clamping groove is arranged on a first friction layer, the side wall of the buckle is provided with a second friction layer, the buckle is clamped into the clamping groove, the first friction layer is in contact with the second friction layer, after the buckle is clamped into the clamping groove, a limited space composed of the bottom wall of the convex ring and the ring wall of the test tube is formed at the bottom of the clamping groove, the end surface of the buckle is allowed to be touched by fingers and to act on the buckle to move relative to the clamping groove, while the end surface of the buckle of other nucleic acid collection tubes is difficult to be touched and to act on the end surface of the buckle, the nucleic acid collection tube is ensured not to be opened and to leak due to collision with each other, and a nucleic acid detection device is also provided.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, and in particular to a nucleic acid collection tube and a nucleic acid detection device. Background Technology

[0002] As the first line of defense against imported cases, an important task for customs is to improve nucleic acid testing capacity to meet the testing requirements of inbound personnel and animals (such as sheep).

[0003] Nucleic acid collection tubes are already available. After collecting the nucleic acid sample, the cap must be unscrewed, a cotton swab inserted into the tube with the tip against one side of the tube wall and the middle against the other side. The base of the swab is then pressed down, causing the tip to break off inside the tube. The cap is then screwed back on, and the sample is sent for testing. This frequent unscrewing and screwing of the cap severely impacts testing efficiency.

[0004] For this reason, see Figure 4 , Figure 5 The applicant has initially designed a nucleic acid collection tube, including a test tube 10, a cap 20, a first rotating seat 30, and a second rotating seat 40. The test tube 10 is open at the top and closed at the bottom. The cap 20 is located at the top of the test tube 10 and rotates relative to the test tube 10. The lower end face of the cap 20 is in contact with the upper end face of the test tube 10 so that the test tube 10 and the cap 20 together form a sealed cavity. The first rotating seat 30 is fixed to the upper end face of the test tube 10 and is cylindrical. The axis of the first rotating seat 30 is parallel to the upper end face of the test tube 10. The second rotating seat 40 is fixed to one side of the cap 20 and is cylindrical. The axis of the second rotating seat 40 is parallel to the lower end face of the cap 20. The first rotating seat 30 and the second rotating seat 40 are coaxially rotatably connected. The test tube 10 and the cap 20 are hinged by the first rotating seat 30 and the second rotating seat 40. Under the action of the first rotating seat 30 and the second rotating seat 40, the cap 20 rotates relative to the test tube 10, and the lower end face of the cap 20 fits against the upper end face of the test tube 10, resulting in a good sealing effect of the tube cavity.

[0005] However, during the transportation of nucleic acid collection tubes, the tubes may collide with each other, which may cause the cap 20 to come into contact with the tubes. Even a slight rotation of the cap 20 relative to the test tube 10 may cause the test tube 10 to leak. Summary of the Invention

[0006] In view of this, and to address the aforementioned shortcomings, it is necessary to propose a nucleic acid collection tube.

[0007] It is also necessary to propose a nucleic acid testing device.

[0008] A nucleic acid collection tube includes a test tube, a raised ring, a cap, and a buckle. The lower end of the test tube is closed, and the upper end is open. A raised ring is provided near the upper ring wall of the test tube, and the raised ring has a groove. The groove is trapezoidal, and the opening of the groove gradually increases from the upper end to the lower end of the test tube. One side of the cap is hinged to the test tube. The lower end face of the cap and the upper end face of the test tube are both smooth planes. The cap can rotate relative to the test tube, and the lower end face of the cap fits against the upper end face of the test tube so that the test tube and the cap together form a sealed cavity. The upper end of the buckle is hinged to the other side of the cap, and the shape of the lower end of the buckle matches the shape of the groove. The side wall of the groove is provided with a first friction layer, and the side wall of the buckle is provided with a second friction layer. The buckle is engaged in the groove, and the first friction layer and the second friction layer are in contact to form resistance to the movement of the buckle relative to the groove.

[0009] Preferably, the nucleic acid collection tube includes a hinge piece, which is an elastic elongated strip. A hinge piece is provided on each side of the tube cap. One end of the hinge piece is connected to a protruding ring or a snap fastener, and the other end of the hinge piece is connected to the tube cap.

[0010] Preferably, the nucleic acid collection tube includes a first rotating seat and a second rotating seat. The first rotating seat is fixed to the upper end face of the test tube and is cylindrical. The axis of the first rotating seat is parallel to the upper end face of the test tube. The second rotating seat is fixed to one side of the tube cap and is cylindrical. The axis of the second rotating seat is parallel to the lower end face of the tube cap. The first rotating seat and the second rotating seat are coaxially rotatably connected, and the diameters of the first rotating seat and the second rotating seat are equal.

[0011] Preferably, the upper end face of the test tube is tangent to the first rotating seat, and the lower end face of the tube cap is tangent to the second rotating seat.

[0012] Preferably, the nucleic acid collection tube includes a cylindrical pin, the first rotating seat is provided with a first pin hole, the second rotating seat is provided with a second pin hole, the first pin hole and the second pin hole are coaxial, the cylindrical pin passes through the first pin hole and the second pin hole, and the cylindrical pin is press-fitted with the first pin hole and the second pin hole.

[0013] Preferably, there are two first rotating seats, and the second rotating seat is disposed between the two first rotating seats.

[0014] A nucleic acid testing device, including a nucleic acid collection tube.

[0015] Preferably, the nucleic acid detection device further includes a droplet generator.

[0016] Preferably, the nucleic acid detection device further includes a droplet analyzer.

[0017] Beneficial effects: After the buckle is inserted into the slot, a limited space is formed at the bottom of the slot, consisting of the bottom wall of the convex ring and the ring wall of the test tube. This allows fingers to touch the end face of the buckle and move it relative to the slot. Other nucleic acid collection tubes have difficulty touching the end face of the buckle and applying force to it, ensuring that the nucleic acid collection tubes collide with each other without opening the tube cap and causing leakage. Attached Figure Description

[0018] Figure 1 This is a left view of the nucleic acid collection tube.

[0019] Figure 2 This is the front view of the nucleic acid collection tube.

[0020] Figure 3 This is a bottom view of the nucleic acid collection tube.

[0021] Figure 4 This is an isometric view of the preliminary design of the nucleic acid collection tube.

[0022] Figure 5 This is a magnified view of a portion of the preliminary design of the nucleic acid collection tube.

[0023] In the diagram: test tube 10, tube cap 20, first rotating seat 30, second rotating seat 40, convex ring 50, buckle 60, hinge piece 80. Detailed Implementation

[0024] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] See Figures 1 to 3 This utility model provides a nucleic acid collection tube, including a test tube 10, a convex ring 50, a tube cap 20, and a buckle 60. The lower end of the test tube 10 is closed, and the upper end of the test tube 10 is open. A convex ring 50 is provided near the upper end of the test tube 10. The convex ring 50 has a groove, which is trapezoidal. The opening of the groove gradually increases from the upper end to the lower end of the test tube 10. One side of the tube cap 20 is hinged to the test tube 10. The lower end surface of the tube cap 20 and the upper end surface of the test tube 10 are both smooth planes. The cap 20 rotates relative to the test tube 10, and the lower end face of the cap 20 fits against the upper end face of the test tube 10, so that the test tube 10 and the cap 20 together form a sealed cavity. The upper end of the buckle 60 is hinged to the other side of the cap 20, and the shape of the lower end of the buckle 60 matches the shape of the slot. The side wall of the slot is provided with a first friction layer, and the side wall of the buckle 60 is provided with a second friction layer. The buckle 60 is inserted into the slot, and the first friction layer and the second friction layer contact each other to form resistance to the movement of the buckle 60 relative to the slot.

[0026] The trapezoidal block cut from the convex ring 50 can be directly used as the buckle 60 without generating waste. During cutting, a first friction layer is naturally formed on the slot, and a second friction layer is naturally formed on the buckle 60. Of course, the first friction layer and the second friction layer can be attached separately.

[0027] Beneficial effects: After the buckle 60 is inserted into the slot, a limited space is formed at the bottom of the slot by the bottom wall of the convex ring 50 and the ring wall of the test tube 10. This allows the finger to touch the end face of the buckle 60 and move the buckle 60 relative to the slot. Other nucleic acid collection tubes have difficulty touching the end face of the buckle 60 and applying force to the end face of the buckle 60, ensuring that the nucleic acid collection tubes collide with each other without opening the tube cap 20 and causing leakage.

[0028] See Figures 1 to 3 Furthermore, the nucleic acid collection tube includes a hinge piece 80, which is an elastic elongated strip. A hinge piece 80 is provided on each side of the tube cap 20. One end of the hinge piece 80 is connected to the protruding ring 50 or the buckle 60, and the other end of the hinge piece 80 is connected to the tube cap 20.

[0029] See Figure 4 , Figure 5 Furthermore, the nucleic acid collection tube includes a first rotating seat 30 and a second rotating seat 40. The first rotating seat 30 is fixed to the upper end face of the test tube 10 and is cylindrical. The axis of the first rotating seat 30 is parallel to the upper end face of the test tube 10. The second rotating seat 40 is fixed to one side of the tube cap 20 and is cylindrical. The axis of the second rotating seat 40 is parallel to the lower end face of the tube cap 20. The first rotating seat 30 and the second rotating seat 40 are coaxially rotatably connected, and the diameters of the first rotating seat 30 and the second rotating seat 40 are equal.

[0030] See Figure 4 , Figure 5 Furthermore, the upper end face of the test tube 10 is tangent to the first rotating seat 30, and the lower end face of the tube cap 20 is tangent to the second rotating seat 40.

[0031] See Figure 4 , Figure 5 Furthermore, the nucleic acid collection tube includes a cylindrical pin, a first pin hole on the first rotating seat 30, and a second pin hole on the second rotating seat 40. The first pin hole and the second pin hole are coaxial, and the cylindrical pin passes through the first pin hole and the second pin hole, with the cylindrical pin and the first pin hole and the second pin hole having an interference fit.

[0032] See Figure 4 , Figure 5 Furthermore, there are two first rotating seats 30, and a second rotating seat 40 is located between the two first rotating seats 40.

[0033] The cap 20 is connected to the test tube 10 by a cylindrical pin. The lower end face of the cap 20 fits fully with the upper end face of the test tube 10, improving the sealing performance of the cap 20 to the test tube 10.

[0034] This utility model provides a nucleic acid detection device, including a nucleic acid collection tube.

[0035] In one embodiment, a suction cup is provided at the bottom of the test tube 10, and the nucleic acid detection device also includes a storage plate, which is a rectangular plate with a smooth surface on its upper surface.

[0036] Furthermore, the nucleic acid testing device also includes a droplet generator.

[0037] Furthermore, the nucleic acid testing device also includes a droplet analyzer.

[0038] Nucleic acid collection tubes are used to collect nucleic acid samples; storage trays are used to hold several collected nucleic acid collection tubes; transport boxes are used to load the storage trays full of nucleic acid collection tubes and transport them to a designated location for extraction and preparation of reaction solutions; droplet generators are used to generate 20,000 nanoliter-sized droplets from the reaction solution, each droplet containing or not containing nucleic acid target molecules, each droplet acting as an independent PCR reactor for PCR amplification; droplet analyzers are used to detect each droplet individually, with a fluorescent signal interpreted as "1" and no fluorescent signal interpreted as "0". Finally, based on the Poisson distribution principle and the proportion of positive droplets, the concentration of the target molecule to be tested is calculated by the analysis software.

[0039] The droplet generator is specifically the QX200 droplet generator, and the droplet analyzer is specifically the QX200 droplet analyzer. The QX200 droplet analyzer and the QX200 droplet generator together constitute the QX200 Droplet Digital PCR system.

[0040] The modules or units in the device of this utility model embodiment can be merged, divided, or deleted according to actual needs.

[0041] The above-disclosed embodiments are merely preferred embodiments of the present utility model and should not be construed as limiting the scope of the present utility model. Those skilled in the art can understand that implementing all or part of the above-described embodiments and making equivalent changes in accordance with the claims of the present utility model are still within the scope of the utility model.

Claims

1. A nucleic acid collection tube, characterized in that: The device includes a test tube, a raised ring, a cap, and a buckle. The lower end of the test tube is closed, and the upper end is open. A raised ring is provided near the upper ring wall of the test tube, and the raised ring has a groove. The groove is trapezoidal, and the opening of the groove gradually increases from the upper end to the lower end of the test tube. One side of the cap is hinged to the test tube. The lower end face of the cap and the upper end face of the test tube are both smooth planes. The cap can rotate relative to the test tube, and the lower end face of the cap fits against the upper end face of the test tube, so that the test tube and the cap together form a sealed cavity. The upper end of the buckle is hinged to the other side of the cap, and the shape of the lower end of the buckle matches the shape of the groove. The side wall of the groove is provided with a first friction layer, and the side wall of the buckle is provided with a second friction layer. The buckle is engaged in the groove, and the first friction layer and the second friction layer are in contact to form resistance to the movement of the buckle relative to the groove.

2. The nucleic acid collection tube as described in claim 1, characterized in that: The nucleic acid collection tube includes a hinge piece, which is an elastic elongated strip. A hinge piece is provided on each side of the tube cap. One end of the hinge piece is connected to a protruding ring or a buckle, and the other end of the hinge piece is connected to the tube cap.

3. The nucleic acid collection tube as described in claim 1, characterized in that: The nucleic acid collection tube includes a first rotating seat and a second rotating seat. The first rotating seat is fixed to the upper end face of the test tube and is cylindrical. The axis of the first rotating seat is parallel to the upper end face of the test tube. The second rotating seat is fixed to one side of the tube cap and is cylindrical. The axis of the second rotating seat is parallel to the lower end face of the tube cap. The first rotating seat and the second rotating seat are coaxially rotatably connected and have the same diameter.

4. The nucleic acid collection tube as described in claim 3, characterized in that: The upper end face of the test tube is tangent to the first rotating seat, and the lower end face of the tube cap is tangent to the second rotating seat.

5. The nucleic acid collection tube as described in claim 4, characterized in that: The nucleic acid collection tube includes a cylindrical pin. The first rotating seat has a first pin hole, and the second rotating seat has a second pin hole. The first pin hole and the second pin hole are coaxial. The cylindrical pin passes through the first pin hole and the second pin hole, and the cylindrical pin is press-fitted with the first pin hole and the second pin hole.

6. The nucleic acid collection tube as described in claim 5, characterized in that: There are two first rotating seats, and a second rotating seat is located between the two first rotating seats.

7. A nucleic acid detection device, characterized in that: Includes the nucleic acid collection tube as described in claim 1.

8. The nucleic acid detection device as described in claim 7, characterized in that: The nucleic acid detection device also includes a droplet generator.

9. The nucleic acid detection device as described in claim 8, characterized in that: The nucleic acid detection device also includes a droplet analyzer.

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