A sampling device suitable for use with a bioreactor

Abstract

The utility model discloses a kind of sampling devices suitable for bioreactor, including biological reaction tank body, the top of the biological reaction tank body is fixed with sealing cover plate by bolt, the inside of the biological reaction tank body is provided with stirring mechanism, the top of the sealing cover plate is provided with mounting mechanism, the mounting mechanism includes mounting support, installation sliding sleeve and butt flange A, the inner wall of the installation sliding sleeve is provided with sampling mechanism;The outside of the installation sliding sleeve is provided with lifting mechanism, the bottom of the mounting mechanism is provided with sealing mechanism;Sampling mechanism is driven to move down by lifting mechanism, material is sampled by sampling mechanism in multiple stages, improve the efficiency of sampling operation, before and after sampling operation, biological reaction tank body is sealed by sealing mechanism, can guarantee the sealing property of bioreactor when sampling operation, avoid miscellaneous bacteria to cause influence to the material inside bioreactor, improve the quality of sampling operation.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor technology, and in particular to a sampling device suitable for bioreactors. Background Technology

[0002] In the biopharmaceutical industry, the technology of expressing specific proteins, interferons, monoclonal antibodies, and vaccines through large-scale in vitro culture of animal cells has become quite mature. Bioreactors have become the primary medium for large-scale in vitro culture. During cell culture or fermentation, aseptic sampling of the bioreactor is necessary to obtain various important detection data, enabling effective control of each reaction stage.

[0003] Compared with existing technologies, the existing sampling devices have the following problems: they lack flexibility in use and cannot guarantee the sealing of the bioreactor during sampling operations, leading to external microorganisms contaminating the materials inside the bioreactor and affecting the quality of sampling operations. Secondly, existing sampling devices can only perform sampling operations at different depths, resulting in low efficiency and poor practicality. Therefore, we propose a sampling device suitable for bioreactors to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a sampling device suitable for bioreactors.

[0005] The present invention solves its technical problem through the following technical solution: it includes a bioreactor, the top of which is fixed with a sealing cover plate by bolts, a stirring mechanism is provided inside the bioreactor, an installation mechanism is provided on the top of the sealing cover plate, the installation mechanism includes an installation bracket, the installation bracket is fixed to the top of the sealing cover plate by bolts, an installation sliding sleeve is fixed on one side of the installation bracket, a docking flange A is fixed at the bottom of the installation sliding sleeve, and a sampling mechanism is provided on the inner wall of the installation sliding sleeve; The sampling mechanism includes an installation sleeve that is slidably connected to the inner wall of an installation slide sleeve. A slot is provided on one side of the installation sleeve. A servo motor B is fixed to the inner wall of the installation sleeve. A drive shaft B is provided at the output end of the servo motor B. A sampling cylinder is fixed to the bottom of the drive shaft B. Multiple partition plates are fixed to the inner wall of the sampling cylinder. Multiple feed slots are provided on the side wall of the sampling cylinder. A lifting mechanism is provided on the outer side of the installation slide sleeve. A sealing mechanism is provided at the bottom of the installation mechanism.

[0006] As a further improvement of this invention, a support frame is fixed to the bottom of the bioreactor.

[0007] As a further improvement of this utility model, a control panel is provided on one side of the support frame.

[0008] As a further embodiment of this utility model: an inlet pipe is fixed to the outer side of the sealing cover, and a discharge pipe is fixed to the center of the bottom of the bioreactor.

[0009] As a further embodiment of this utility model: the stirring mechanism includes a motor mounting base, which is fixed to the top of the sealing cover plate by bolts. A servo motor A is fixed to the top of the motor mounting base by bolts. A drive shaft A is provided at the output end of the servo motor A. A stirring frame is fixed to the outside of the drive shaft A.

[0010] As a further embodiment of this utility model: the lifting mechanism includes a mounting block, which is fixed to the side wall of the mounting sleeve by bolts. A mounting groove is fixed on one side of the mounting block, and a drive motor is fixed to the top of the mounting groove by bolts. A lead screw is provided at the output end of the drive motor, and a sliding seat is provided on the outside of the lead screw. An insert block is fixed on one side of the sliding seat, and a positioning pin is slidably connected to the inner wall of the insert block.

[0011] As a further embodiment of this utility model: the sealing mechanism includes a mounting housing, which is fixed to the top of the sealing cover plate by bolts. A mating flange B is fixed to the top of the mounting housing, and the mating flange B is fixed to the mating flange A by bolts. A motor mounting bracket is fixed to the outer side of the mounting housing, and a geared motor is fixed to the inner wall of the motor mounting bracket. A drive rod is provided at the output end of the geared motor, and a sealing ball core is fixed to one end of the drive rod. The sealing ball core is rotatably connected to the mounting housing.

[0012] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are: 1. It is equipped with an installation mechanism and a sampling mechanism. The sampling mechanism is driven by a lifting mechanism to slide on the inner wall of the installation sleeve. The sampling mechanism performs multi-stage sampling operations on the material, which improves the efficiency of the sampling operation. 2. Before and after the sampling operation, the bioreactor is sealed by the sealing mechanism, and the sliding sleeve is used to slide and seal the installation sleeve. This ensures the airtightness of the bioreactor during the sampling operation, avoids the influence of miscellaneous bacteria on the materials inside the bioreactor, improves the quality of the sampling operation, and thus improves the practicality of the device. 3. The sampling cylinder is moved into the bioreactor by a lifting mechanism, which can drive the sampling mechanism. Similarly, the sampling mechanism can be quickly removed by taking the installation sleeve off the insert block, making it convenient to use the sample. Attached Figure Description

[0013] Figure 1 A schematic diagram of an isometric structure according to an embodiment of the present invention is shown; Figure 2 A schematic diagram of an isometric sectional view of a structure according to an embodiment of the present invention is shown; Figure 3 The present invention provides an embodiment of the present invention. Figure 2 Enlarged structural diagram of part A in the middle; Figure 4 The present invention provides an embodiment of the present invention. Figure 2 Enlarged structural diagram of section B in the middle; Figure 5 The present invention provides an embodiment of the present invention. Figure 2 Enlarged structural diagram of section C in the middle; Figure 6 A schematic diagram of the front cross-sectional structure according to an embodiment of the present invention is shown; Figure 7 A partial structural schematic diagram according to an embodiment of the present invention is shown.

[0014] Legend: 100 Bioreactor body, 110 Support frame, 120 Control panel, 130 Sealing cover, 140 Feed pipe, 150 Discharge pipe, 210 Motor mounting base, 220 Servo motor A, 221 Drive shaft A, 230 Stirring rack, 310 Mounting bracket, 320 Mounting sleeve, 330 Butt flange A, 410 Mounting sleeve, 420 Groove, 430 Servo motor B, 440 Drive shaft B, 450 Sampling cylinder, 451 Divider plate, 460 Feed chute, 510 Mounting block, 520 Mounting groove, 530 Drive motor, 531 Lead screw, 540 Sliding seat, 550 Insert block, 560 Positioning pin, 610 Mounting shell, 620 Butt flange B, 630 Motor mounting bracket, 640 Gear motor, 641 Drive rod, 650 Sealing ball core. Detailed Implementation

[0015] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0016] In the description of this utility model, "multiple" means two or more, unless otherwise explicitly specified.

[0017] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "sleeved / connected," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0018] Please see Figure 1-7 This utility model provides a technical solution: it includes a bioreactor 100, a support frame 110 fixed at the bottom of the bioreactor 100, a control panel 120 provided on one side of the support frame 110, a sealing cover plate 130 fixed at the top of the bioreactor 100 by bolts, a stirring mechanism provided inside the bioreactor 100, an installation mechanism provided at the top of the sealing cover plate 130, the installation mechanism including an installation bracket 310 and an installation sliding sleeve 320, a docking flange A330 fixed at the bottom of the installation sliding sleeve 320, and a sampling mechanism provided on the inner wall of the installation sliding sleeve 320; The sampling mechanism includes an installation sleeve 410, a slot 420, a servo motor B430, and a drive shaft B440. A sampling cylinder 450 is fixed to the bottom of the drive shaft B440. Multiple partition plates 451 are fixed to the inner wall of the sampling cylinder 450. Multiple feed slots 460 are opened on the side wall of the sampling cylinder 450. A lifting mechanism is provided on the outer side of the installation sleeve 320, and a sealing mechanism is provided at the bottom of the installation mechanism. With the installation mechanism and the sampling mechanism, the sampling mechanism is driven to slide on the inner wall of the installation sleeve 320. The sampling mechanism performs multi-stage sampling operations on the material, which improves the efficiency of the sampling operation. Before and after the sampling operation, the sealing mechanism seals the bioreactor 100. Combined with the sliding seal of the installation sleeve 320 on the installation sleeve 410, the sealing of the bioreactor during the sampling operation can be guaranteed, avoiding the influence of miscellaneous bacteria on the material inside the bioreactor, improving the quality of the sampling operation, and thus improving the practicality of the device.

[0019] Specifically, an inlet pipe 140 is fixed to the outside of the sealing cover plate 130, and a discharge pipe 150 is fixed to the center of the bottom of the bioreactor 100; materials are added through the inlet pipe 140 and discharged through the discharge pipe 150.

[0020] Specifically, the stirring mechanism includes a motor mounting base 210, which is fixed to the top of the sealing cover plate 130 by bolts. A servo motor A220 is fixed to the top of the motor mounting base 210 by bolts. A drive shaft A221 is provided at the output end of the servo motor A220, and a stirring frame 230 is fixed to the outside of the drive shaft A221. By providing a stirring mechanism, the servo motor A220 drives the drive shaft A221 to rotate, and the rotation of the drive shaft A221 drives the stirring frame 230 to rotate, thereby stirring the material through the stirring frame 230 and achieving efficient reaction of the material.

[0021] Specifically, the lifting mechanism includes a mounting block 510, which is fixed to the side wall of the mounting sleeve 320 by bolts. A mounting groove 520 is fixed to one side of the mounting block 510, and a drive motor 530 is fixed to the top of the mounting groove 520 by bolts. A lead screw 531 is provided at the output end of the drive motor 530, and a sliding seat 540 is provided on the outer side of the lead screw 531. An insert block 550 is fixed to one side of the sliding seat 540, and a positioning pin 560 is slidably connected to the inner wall of the insert block 550. By setting up a lifting mechanism to drive the sampling cylinder 450 to move into the bioreactor 100, the sampling mechanism can be driven. Similarly, by removing the mounting sleeve 410 from the insert block 550, the sampling mechanism can be quickly removed for convenient use of samples.

[0022] Specifically, the sealing mechanism includes a mounting housing 610, which is bolted to the top of the sealing cover plate 130. A docking flange B620 is fixed to the top of the mounting housing 610, and the docking flange B620 is bolted to the docking flange A330. A motor mounting bracket 630 is fixed to the outer side of the mounting housing 610, and a geared motor 640 is fixed to the inner wall of the motor mounting bracket 630. A drive rod 641 is provided at the output end of the geared motor 640, and a sealing ball core 650 is fixed to one end of the drive rod 641. The sealing ball core 650 is rotatably connected to the mounting housing 610. By setting up the sealing mechanism, after the material is sampled, the mounting housing 610 is sealed by the sealing ball core 650, thereby achieving the sealing operation of the bioreactor 100. Combined with the sealing operation of the sampling mechanism by the mounting mechanism, the sealing of the bioreactor can be guaranteed during the sampling operation, avoiding the influence of miscellaneous bacteria on the material inside the bioreactor and improving the quality of the sampling operation.

[0023] Working Principle: During use, the device is stably supported by a support frame 110, and its operation is controlled by a control panel 120. Materials are added through an inlet pipe 140 and discharged through a discharge pipe 150. A servo motor A220 drives a drive shaft A221 to rotate, which in turn rotates a stirring frame 230 to agitate the materials, achieving efficient reaction. The mounting sleeve 410 is installed on the insert block 550 and fixed thereby by a positioning pin 560. A drive motor 530 drives a lead screw 531 to rotate, which in turn moves a sliding seat 540 up and down. The sliding seat 540, through the insert block 550, moves the mounting sleeve 410 up and down, thereby moving the sampling cylinder 450 into the bioreactor 100. The servo motor... Motor B430 drives drive shaft B440 to rotate, which in turn drives sampling cylinder 450 to rotate, rotating feed trough 460 to one side of slot 420. Dividing sampling cylinder 450 into multiple storage chambers via partition plate 451, materials of different depths enter sampling cylinder 450 through multiple feed troughs 460, enabling multi-stage sampling. After sampling, mounting sleeve 410 is removed from insert block 550, allowing for quick removal of the sampling mechanism and convenient sample use. Before and after sampling, geared motor 640 drives drive rod 641 to rotate, which in turn drives sealing ball core 650 to rotate, sealing mounting shell 610 and thus achieving sealing of bioreactor 100. Combined with the sealing operation of the sampling mechanism by the mounting mechanism, this ensures the bioreactor's airtightness during sampling.

[0024] The motor involved in the embodiments, its matching control system, electromagnetic switch and pipeline circuit can also be provided by the manufacturer. Apart from that, the power supply module, circuit and electronic components and control module involved in this utility model are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated. The content protected by this utility model does not involve any improvement to the internal structure and method.

[0025] Although the present invention discloses embodiments and accompanying drawings, those skilled in the art will understand that various substitutions, variations and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments and accompanying drawings.

Claims

1. A sampling device suitable for bioreactors, characterized in that, The system includes a bioreactor (100), the top of which is fixed with a sealing cover (130) by bolts. The bioreactor (100) is equipped with a stirring mechanism inside. The top of the sealing cover (130) is equipped with an installation mechanism, which includes an installation bracket (310). The installation bracket (310) is fixed to the top of the sealing cover (130) by bolts. An installation sleeve (320) is fixed to one side of the installation bracket (310). A docking flange A (330) is fixed to the bottom of the installation sleeve (320). A sampling mechanism is provided on the inner wall of the installation sleeve (320). The sampling mechanism includes an installation sleeve (410), which is slidably connected to the inner wall of an installation slide sleeve (320). A slot (420) is provided on one side of the installation sleeve (410). A servo motor B (430) is fixed on the inner wall of the installation sleeve (410). A drive shaft B (440) is provided at the output end of the servo motor B (430). A sampling cylinder (450) is fixed at the bottom of the drive shaft B (440). Multiple partition plates (451) are fixed on the inner wall of the sampling cylinder (450). Multiple feed slots (460) are provided on the side wall of the sampling cylinder (450). A lifting mechanism is provided on the outer side of the installation slide sleeve (320). A sealing mechanism is provided at the bottom of the installation mechanism.

2. A sampling device suitable for bioreactors according to claim 1, characterized in that, The bottom of the bioreactor (100) is fixed with a support frame (110).

3. A sampling device suitable for bioreactors according to claim 2, characterized in that, A control panel (120) is provided on one side of the support frame (110).

4. A sampling device suitable for bioreactors according to claim 3, characterized in that, An inlet pipe (140) is fixed to the outside of the sealing cover (130), and a discharge pipe (150) is fixed to the center of the bottom of the bioreactor (100).

5. A sampling device suitable for bioreactors according to claim 1, characterized in that, The stirring mechanism includes a motor mounting base (210), which is fixed to the top of the sealing cover plate (130) by bolts. A servo motor A (220) is fixed to the top of the motor mounting base (210) by bolts. A drive shaft A (221) is provided at the output end of the servo motor A (220). A stirring frame (230) is fixed to the outside of the drive shaft A (221).

6. A sampling device suitable for bioreactors according to claim 1, characterized in that, The lifting mechanism includes a mounting block (510), which is fixed to the side wall of the mounting sleeve (320) by bolts. A mounting groove (520) is fixed on one side of the mounting block (510). A drive motor (530) is fixed to the top of the mounting groove (520) by bolts. A lead screw (531) is provided at the output end of the drive motor (530). A sliding seat (540) is provided on the outside of the lead screw (531). An insert (550) is fixed on one side of the sliding seat (540). A positioning pin (560) is slidably connected to the inner wall of the insert (550).

7. A sampling device suitable for bioreactors according to claim 1, characterized in that, The sealing mechanism includes a mounting housing (610), which is fixed to the top of the sealing cover plate (130) by bolts. A docking flange B (620) is fixed to the top of the mounting housing (610), and the docking flange B (620) is fixed to the docking flange A (330) by bolts. A motor mounting bracket (630) is fixed to the outside of the mounting housing (610), and a geared motor (640) is fixed to the inner wall of the motor mounting bracket (630). A drive rod (641) is provided at the output end of the geared motor (640), and a sealing ball core (650) is fixed to one end of the drive rod (641). The sealing ball core (650) is rotatably connected to the mounting housing (610).

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