A device for monitoring the temperature of a fungicide

Abstract

The utility model discloses a kind of fungus-destroying liquid temperature monitoring devices, belong to biological detection equipment technical field, including the pipeline body of two ends opening, pipeline body is equipped with the recess for enriching fungus-destroying liquid, the recess is equipped with the probe thermometer for detecting fungus-destroying liquid temperature directly above, screen between the recess and the import of pipeline body is equipped for intercepting the foam in fungus-destroying liquid, solve the problem that fungus-destroying liquid temperature detection work is complicated and exists in prior art temperature detection result inaccurate.

Description

Technical Field

[0001] This utility model belongs to the field of biological detection equipment technology, specifically relating to a device for monitoring the temperature of a bacterial lysate. Background Technology

[0002] The lysed solution is produced by mixing bacterial cells with a buffer solution and breaking down the bacterial cells through repeated cycles of compression and other processes. This lysed solution provides the raw material for purifying the target protein. The purification process to obtain the target protein from the lysed solution generally needs to be carried out within a specific temperature range to ensure the activity of the purified protein; therefore, it is necessary to monitor the temperature of the lysed solution during this process.

[0003] Currently, most methods for monitoring the temperature of the sterilization solution employ traditional techniques. A thermometer is inserted into the pipeline supplying the solution, and to avoid damaging the probe, the thermometer is designed to be kept at a certain distance from the tank wall, directly measuring the temperature of the solution. This method generally has the following problems: ① When detecting temperature, if the level of the sterilization solution in the pipeline is low, the temperature detection result may be inaccurate or the temperature of the air in the pipeline may be measured directly. ② During the sterilization process, the degree of sterilization is low in a single operation. Therefore, the bacterial solution needs to be circulated multiple times in the pipeline and enter the homogenizer multiple times for sterilization. The bacterial solution exiting the homogenizer will generate a large amount of foam due to factors such as protein dissolution in the buffer solution and mechanical pressure. If the foam adheres to the temperature probe, it will also affect the accuracy of the test results. ③Because conventional pipes are mostly made of stainless steel (non-transparent), the thickness and diameter of the pipe tank walls vary, which requires the preparation of probes of different lengths. Because the pipes are not transparent, it is very difficult to accurately control the insertion position of the probes.

[0004] Therefore, it is necessary to introduce a simple, easy-to-operate, and more professional sterilization solution temperature detection device to solve the above problems. Utility Model Content

[0005] The present invention aims to solve the problems of complicated temperature detection of the sterilization solution and inaccurate temperature detection results in the prior art.

[0006] To achieve the above-mentioned objectives, the technical solution of this utility model is as follows: A device for monitoring the temperature of a lysate includes a pipe body with openings at both ends. The pipe body has a groove for collecting the lysate. A probe thermometer for detecting the temperature of the lysate is located directly above the groove. A screen for intercepting foam in the lysate is located between the groove and the inlet of the pipe body.

[0007] Furthermore, the probe of the probe thermometer extends into the pipe body through the pipe wall, and the probe is connected to the pipe body through a threaded structure.

[0008] Furthermore, when the probe thermometer is inserted to its deepest point into the pipe body, the tip of the probe is flush with the inner wall of the pipe body on the opposite side, or slightly extends into the groove.

[0009] Furthermore, the probe thermometer is electrically connected to an alarm.

[0010] Furthermore, the longitudinal section of the groove is "V" shaped or "U" shaped.

[0011] Furthermore, the screen is positioned close to the sloping portion of the groove.

[0012] Furthermore, the screen mesh is evenly distributed with sieve holes of 1~2mm.

[0013] Furthermore, the pipe body is a transparent glass tube, and an insulation sleeve is provided on the outside of the transparent glass tube.

[0014] Furthermore, the screen comprises multiple screens.

[0015] Furthermore, the monitoring device is connected to other pipelines via clamps or other structures.

[0016] The beneficial effects of this utility model are: I. This utility model proposes a cleverly designed and simple temperature monitoring device for the lysed solution. In use, it is horizontally installed at the connection point of other pipes and then fixed in place by clamps or similar structures. The screen of the monitoring device can intercept foam in the lysed solution. The bacterial solution flows through the pipe body and accumulates in the groove, facilitating accurate real-time temperature measurement of the lysed solution by a probe thermometer installed on the pipe body above the groove. This solves the problem in existing technologies where inaccurate temperature detection of the lysed solution affects the purification results of the target protein. Furthermore, this structure is easy to operate and clean, has low cost, is simple to assemble, and is easy to promote and use.

[0017] Second, in this utility model, the probe of the probe thermometer penetrates the pipe body wall and extends into its interior. The two are connected by a threaded structure. This threaded structure not only realizes the detachable design, making it convenient to clean the probe thermometer after use, but also stably fixes it on the pipe body. At the same time, the depth of the probe extending into the pipe body can be finely adjusted by adjusting the thread. The probe thermometer's performance is guaranteed from three aspects: ease of cleaning, stable installation, and optimized measurement position.

[0018] Third, in this utility model, when the probe thermometer is inserted to the deepest point into the pipe body, the probe tip is flush with the inner wall of the pipe body on the opposite side. With this structural design, when the sterilization liquid is introduced into the pipe body, if the liquid level in the groove is lower than the tip of the probe (the lowest end), the probe thermometer cannot detect the temperature of the sterilization liquid, which means that there is no liquid passing through the pipe at this time, and can prompt the staff to arrange the next step of work.

[0019] IV. In this utility model, the probe thermometer is electrically connected to an alarm. When the probe thermometer detects that the temperature of the sterilization solution exceeds the preset value, it can issue an alarm and alert the staff.

[0020] V. In this utility model, the longitudinal section of the groove is preferably "V" or "U" shaped to ensure that the bacterial lysate is enriched while also facilitating cleaning.

[0021] VI. In this utility model, the screen and the sloping part of the groove are set close together, which makes it easy for the sterilization liquid after being filtered by the screen to enter the groove in time, and avoids foaming again.

[0022] VII. In this utility model, the screen is preferably evenly distributed with 1-2mm sieve holes, and the pipe body is a transparent glass tube, which also facilitates the staff to observe the logistics situation. Attached Figure Description

[0023] Figure 1 This is a three-dimensional structural diagram of the sterilization solution temperature monitoring device.

[0024] Figure 2 yes Figure 1 Perspective view.

[0025] Figure 3 This is a side view of the sterilization solution temperature monitoring device.

[0026] Figure 4 yes Figure 3 BB cross-sectional view.

[0027] Figure 5 This is a cross-sectional view (a) of another embodiment of the sterilization solution temperature monitoring device.

[0028] Figure 6 This is a cross-sectional view (II) of another embodiment of the sterilization solution temperature monitoring device.

[0029] Figure 7 This is a cross-sectional view (III) of another embodiment of the sterilization solution temperature monitoring device.

[0030] Figure 8 This is a cross-sectional view (four) of another embodiment of the sterilization solution temperature monitoring device.

[0031] Among them, 1. Pipe body; 2. Groove; 3. Probe thermometer; 4. Screen; 5. Threaded structure; 6. Alarm; 1.1 Inlet; 1.2 Outlet; 1.3 Insulation sleeve; 3.1 Probe; 4.1 Screen hole. Detailed Implementation

[0032] The present invention will be further described in detail below with reference to the embodiments, but the implementation of the present invention is not limited thereto.

[0033] Example 1 A device for monitoring the temperature of a bacterial lysate, reference Figures 1-4 It includes a pipe body 1 with openings at both ends, a groove 2 for enriching the sterilization liquid inside the pipe body 1, a probe thermometer 3 for detecting the temperature of the sterilization liquid directly above the groove 2, and a screen 4 for intercepting foam in the sterilization liquid between the groove 2 and the inlet 1.1 of the pipe body 1.

[0034] In use, the two ends of the monitoring device’s pipe body 1 are connected to other pipes via clamp structures.

[0035] The lysed solution formed by mixing bacterial cells and buffer solution and physically disrupting them needs to be maintained within a certain temperature range to ensure its biological activity. Therefore, it is necessary to monitor the temperature of the lysed solution in real time and take appropriate temperature control measures in a timely manner.

[0036] The lysing solution enters the main body of the pipeline 1 from the inlet 1.1 of the lysing solution temperature monitoring device. Foam in the lysing solution is intercepted by the screen 4 to avoid detection errors. After filtration, the lysing solution passes through the groove 2, which facilitates the placement of the probe thermometer 3 to detect the temperature of the lysing solution, especially when the flow rate is low. Finally, the lysing solution is discharged from the outlet 1.2 of the main body of the pipeline 1 to the next process.

[0037] Example 2 This embodiment is a further optimization of embodiment 1. The difference is that the probe 3.1 of the probe thermometer 3 extends into the pipe body 1 through the pipe wall and is connected to the pipe body 1 through the threaded structure 5.

[0038] refer to Figure 5 The threaded structure 5 not only achieves a detachable design, making it convenient to clean the probe thermometer 3 after use, but also stably fixes it to the pipe body 1. At the same time, the depth of the probe 3.1 inserted into the pipe body 1 can be finely adjusted by adjusting the thread. The probe thermometer 3 is guaranteed to perform well in terms of cleaning convenience, installation stability and measurement position optimization.

[0039] Example 3 This embodiment is a further optimization of embodiment 2. The difference is that when the probe thermometer 3 is inserted to the deepest point into the pipe body 1, the end of the probe 3.1 is flush with or slightly inserted into the inner wall of the pipe body 1 on the opposite side.

[0040] refer to Figure 5With this structural design, when the sterilization liquid is introduced into the pipe body 1, if the liquid level in the groove 2 is lower than the end side (lowest end) of the probe 3.1, the probe thermometer 3 cannot detect the temperature of the sterilization liquid, which means that there is no liquid passing through the pipe at this time, and can prompt the staff to arrange the next step of work.

[0041] Example 4 The only difference between this embodiment and embodiments 1-3 is that the probe thermometer 3 is electrically connected to an alarm 6. (See reference...) Figure 6 When the probe thermometer 3 detects that the temperature of the sterilization solution exceeds the preset value, it can issue an alarm and alert the staff.

[0042] Example 5 The only difference between this embodiment and embodiments 1-4 is that the longitudinal section of groove 2 is either "V"-shaped or "U"-shaped. (See reference) Figure 4 , Figure 4 The illustration shows a groove 2 with a "U"-shaped longitudinal section.

[0043] Example 6 Compared with Examples 1-5, the only difference in this embodiment is that the screen 4 and the inclined portion of the groove 2 are arranged close together. (Refer to...) Figure 7 .

[0044] Example 7 The only difference between this embodiment and embodiments 1-6 is that the sieve 4 has evenly distributed sieve holes 4.1. (Refer to...) Figure 3 The sieve aperture 4.1 should preferably be a round hole with a diameter of 1~2mm or a square hole with a side length of 1~2mm to facilitate the interception of foam in the bacterial lysate.

[0045] Example 8 The only difference between this embodiment and embodiments 1-7 is that the pipe body 1 is a transparent glass tube, and the transparent glass tube is provided with an insulation sleeve 1.3. (Refer to...) Figure 8 This allows staff to monitor the flow of materials passing through the monitoring device, as well as the temperature detection status.

[0046] Example 9 The only difference between this embodiment and embodiments 1-8 is that the sieve includes three parts. (Refer to...) Figure 8 In actual production, the number of screens 4 can be designed according to the amount of air bubbles.

[0047] Example 10 To facilitate public understanding of this solution, this embodiment uses a temperature monitoring device for the sterilization solution with a superior structure as an example to further illustrate this solution.

[0048] refer to Figure 1 , 6The monitoring device includes a pipe body 1 with openings at both ends. The pipe body 1 has a groove 2 for enriching the sterilized liquid. A probe thermometer 3 for detecting the temperature of the sterilized liquid is located directly above the groove 2. A screen 4 for intercepting foam in the sterilized liquid is located between the groove 2 and the inlet 1.1 of the pipe body 1.

[0049] In this embodiment, the probe 3.1 of the probe thermometer 3 penetrates the pipe wall of the pipe body 1 and extends into the pipe body 1. The probe 3.1 is connected to the pipe body 1 through the threaded structure 5. When the probe thermometer 3 extends to the deepest point in the pipe body 1, the end of the probe 3.1 is flush with the inner wall of the pipe body 1 on the opposite side.

[0050] In this embodiment, the probe thermometer 3 is electrically connected to the alarm 6.

[0051] In this embodiment, the longitudinal section of the groove 2 is U-shaped, which facilitates cleaning the inner wall of the pipe body 1. The screen 4 has 1mm sieve holes 4.1 evenly distributed.

[0052] In this embodiment, the pipe body 1 is a transparent glass pipe.

[0053] In this embodiment, the monitoring device is connected to other pipelines via structures such as clamps.

[0054] In use, the two ends of the monitoring device's main pipe 1 are connected to other pipes via clamp structures. The lysed solution formed after the bacterial cells and buffer solution are mixed and physically broken needs to be maintained within a certain temperature range to ensure its biological activity. Therefore, it is necessary to monitor the temperature of the lysed solution in real time and take appropriate temperature control measures in a timely manner.

[0055] The lysing solution enters the main body of the pipeline 1 from the inlet 1.1 of the lysing solution temperature monitoring device. Foam in the lysing solution is intercepted by the screen 4 to avoid detection errors. After filtration, the lysing solution passes through the groove 2, which facilitates the placement of the probe thermometer 3 to detect the temperature of the lysing solution, especially when the flow rate is low. Finally, the lysing solution is discharged from the outlet 1.2 of the main body of the pipeline 1 to the next process.

Claims

1. A device for monitoring the temperature of a bacterial lysate, characterized in that: The pipe body (1) has openings at both ends. The pipe body (1) has a groove (2) for enriching the lysate. A probe thermometer (3) for detecting the temperature of the lysate is located directly above the groove (2). A screen (4) for intercepting foam in the lysate is located between the groove (2) and the inlet (1.1) of the pipe body (1).

2. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The probe (3.1) of the probe thermometer (3) extends into the pipe body (1) through the pipe wall and is connected to the pipe body (1) through the threaded structure (5).

3. The device for monitoring the temperature of the sterilization solution according to claim 2, characterized in that: When the probe thermometer (3) is inserted to the deepest point into the pipe body (1), the end of the probe (3.1) is flush with the inner wall of the pipe body (1) on the opposite side or slightly extends into the groove (2).

4. The device for monitoring the temperature of the sterilization solution according to claim 2, characterized in that: The probe thermometer (3) is electrically connected to an alarm (6).

5. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The longitudinal section of the groove (2) is "V" or "U".

6. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The screen (4) is set close to the slope of the groove (2).

7. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The sieve (4) has sieve holes (4.1) of 1~2mm evenly distributed on it.

8. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The screen (4) comprises multiple screens.

9. The device for monitoring the temperature of the sterilization solution according to claim 1, characterized in that: The pipe body (1) is a transparent glass tube, and an insulation sleeve (1.3) is provided on the outside of the transparent glass tube.

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