A cas12a-based candida albicans rapid nucleic acid detection device

Abstract

The utility model belongs to the field of sample detection, specifically is a kind of white candida albicans rapid nucleic acid detection device based on Cas12a, including nucleic acid detector, nucleic acid detector is provided with assembly slot, assembly slot is provided with detection room, detection room is equipped with detection head, detection room is equipped with reagent conveying mechanism, reagent conveying mechanism includes guide rod, guide rod is assembled with detection room, guide rod is assembled with limit block and guide block, guide block is equipped with mounting pipe, mounting pipe is equipped with movable rod, movable rod is equipped with support block, support block is provided with slot, support block is equipped with connecting rod, connecting rod is equipped with bearing block, support block is provided with socket, bearing block is provided with bearing groove, above-mentioned one kind of white candida albicans rapid nucleic acid detection device based on Cas12a solves nucleic acid detector needing to be detected by manual one by one by the cooperation of insert block and adjusting block, this leads to the problem of low detection efficiency, and manual operation is complicated.

Description

Technical Field

[0001] This utility model relates to the field of sample detection, specifically a rapid nucleic acid detection device for Candida albicans based on Cas12a. Background Technology

[0002] The CRISPR / Cas12a-based nucleic acid detection instrument for Candida albicans is mainly used for the rapid detection of Candida albicans in clinical samples. This device utilizes the CRISPR / Cas12a system to specifically recognize the DNA target of Candida albicans, integrating nucleic acid amplification and cleavage reactions. It is suitable for nucleic acid extraction and detection of clinical samples such as sputum and wound swabs, significantly improving the sensitivity and efficiency of fungal infection diagnosis. Compared to traditional blood culture methods, this technology can complete the detection within hours, significantly shortening the diagnostic cycle. It uses CRISPR RNA to guide the Cas12a protein to accurately recognize the unique ITS2 gene sequence of Candida albicans, avoiding interference from other fungi. Currently, this technology has been applied to the development of nucleic acid detection kits and is planned to be expanded to fields such as environmental monitoring and food safety.

[0003] However, existing nucleic acid detection instruments for Candida albicans based on Cas12a still have the following problems during use: Existing nucleic acid testing instruments require manual insertion of reagent tubes containing Candida albicans Cas12a into the detection head for each test, resulting in low detection efficiency and cumbersome, labor-intensive operation. Therefore, it is necessary to develop a rapid nucleic acid detection device for Candida albicans based on Cas12a to replace the existing rapid nucleic acid detection device for Candida albicans based on Cas12a. Utility Model Content

[0004] To overcome the shortcomings of existing technologies, current nucleic acid testing instruments require manual insertion of reagent tubes containing Candida albicans Cas12a into the detection head for detection, which results in low detection efficiency and cumbersome and labor-intensive manual operation. This invention proposes a rapid nucleic acid detection device for Candida albicans based on Cas12a.

[0005] The technical solution adopted by this utility model to solve its technical problem is: a rapid nucleic acid detection device for Candida albicans based on Cas12a, comprising: A nucleic acid testing instrument, wherein the nucleic acid testing instrument is provided with an assembly slot, a testing chamber is provided on the assembly slot, and multiple testing heads are fixedly mounted on the top surface of the testing chamber; The reagent delivery mechanism includes guide rods. Two guide rods are fixedly assembled to the inner wall of the detection chamber. Limit blocks are fixedly assembled to one end of the two guide rods. Guide blocks are movably assembled on the two guide rods. Installation tubes are symmetrically fixedly assembled on the guide blocks. Movable rods are movably assembled on each installation tube. Support blocks are fixedly assembled to the top of the two movable rods. Slots are provided on the end face of the support blocks. Connecting rods are fixedly assembled to the bottom of the support blocks. Bearing blocks are fixedly assembled to the bottom of the connecting rods. Multiple insertion ports are provided on the support blocks. Multiple bearing grooves are provided on the bearing blocks. The lifting mechanism includes a fixed block, which is fixedly assembled to the inner wall of the testing chamber. Vertical rods are symmetrically fixedly assembled to the bottom of the fixed block. Limit plates are fixedly assembled to the bottom of the two vertical rods. Adjusting blocks are movably assembled on the two vertical rods. Vertical blocks are fixedly assembled to the bottom of the adjusting blocks. The vertical blocks are located on one side of the limit plates. Insert blocks are fixedly assembled to the bottom of the vertical blocks. The insert blocks can be inserted into slots.

[0006] Preferably, the bearing groove is connected to the socket, and the number of sockets is the same as the number of detection heads.

[0007] Preferably, a transmission screw is movably mounted on the nucleic acid detector and the limiting block. The transmission screw is threadedly assembled with the guide block. A driven equal-diameter bevel gear is fixedly mounted on one end of the transmission screw. The driven equal-diameter bevel gear is located on one side of the nucleic acid detector. Assembly blocks are symmetrically fixedly mounted on the back of the nucleic acid detector. An operating motor is fixedly mounted on the assembly block. A rotating rod is fixedly mounted on the output end of the operating motor. An active equal-diameter bevel gear is fixedly mounted on the rotating rod. The active equal-diameter bevel gear and the driven equal-diameter bevel gear are threadedly assembled.

[0008] Preferably, a second sprocket is fixedly mounted on one end of the rotating rod, a rotating groove is provided on the top surface of the assembly groove, a rotating rod is movably mounted on the rotating groove, a rotating block is fixedly mounted on the rotating rod, a box door is fixedly mounted on the rotating block, the box door is movably assembled with the assembly groove, one end of the rotating rod movably passes through the nucleic acid detector, and a first sprocket is fixedly mounted on one end of the rotating rod, with a chain wound around the first sprocket and the second sprocket.

[0009] Preferably, a controller is fixedly mounted on the side of the nucleic acid detector, a first connecting wire is fixedly mounted on the controller, one end of the first connecting wire is fixedly mounted to the operating motor, and a second connecting wire is fixedly mounted on the controller, one end of the second connecting wire is fixedly mounted with a plug.

[0010] Preferably, the fixed block is provided with an operating port located on one side of the vertical rod. Two assembly rods are symmetrically fixedly mounted on the two operating ports, and movable blocks are movably mounted on the two assembly rods. A mounting plate is fixedly mounted on the bottom of the movable block. A fixed plate is fixedly mounted on the end face of the adjusting block, and an adjusting rod is movably mounted on the fixed plate. One end of the adjusting rod is movably mounted to the mounting plate. A traction rod is fixedly mounted on the movable block, movably penetrating through the fixed block and the nucleic acid detector. A positioning block is fixedly mounted on one end of the traction rod, and the positioning block is located on one side of the nucleic acid detector.

[0011] Preferably, the back of the nucleic acid detector is fixedly equipped with a U-shaped slide groove, which is movably assembled with a positioning block. A first locking hole and a second locking hole are provided on the side of the U-shaped slide groove, and both the first and second locking holes are close to the positioning block.

[0012] Preferably, the positioning block has an internal cavity, on which a telescopic spring is fixedly mounted. One end of the telescopic spring is fixedly mounted with a push plate, and a telescopic button is fixedly mounted on the push plate. One end of the telescopic button moves through the positioning block, and one end of the telescopic button is inserted into only one of the first and second locking holes.

[0013] The advantages of this utility model are: This invention involves inserting multiple reagent tubes containing Candida albicans Cas12a into the sockets of a support block, with the bottoms of the reagent tubes resting on the support grooves of the support block. The plug and socket are then connected. Controlling the controller starts the operating motor, whose output drives the active equal-diameter bevel gear on the rotating rod. This active bevel gear drives the driven equal-diameter bevel gear, which in turn drives the transmission screw to rotate on the guide block, causing the guide block to move on two guide rods. Simultaneously, the rotating rod drives the second sprocket to rotate. The chain drive rotates the first sprocket, which in turn rotates the rotating block on the rotating rod in the rotating groove. The rotating block causes the door to flip in the assembly groove, sealing the detection chamber until the insertion port is positioned below the detection head. At this point, the insertion block engages with the slot, raising the support block and causing one end of the movable rod to move inside the mounting tube. This allows the detection head to be inserted into the Candida albicans Cas12a in the reagent tube, enabling the nucleic acid detector to detect Candida albicans Cas12a through the detection head, reducing manual operation and improving detection efficiency. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the front structure of the nucleic acid detection instrument of this utility model; Figure 2 This is a front cross-sectional view of the nucleic acid detection instrument of this utility model; Figure 3 This is a schematic diagram of the back structure of the nucleic acid detection instrument of this utility model; Figure 4 This is a schematic diagram of the reagent delivery mechanism of this utility model; Figure 5 This is a schematic diagram of the lifting mechanism structure of this utility model; Figure 6 This is a cross-sectional view of the positioning block in the lifting mechanism of this utility model.

[0016] In the picture: 10. Nucleic acid testing instrument; 11. Assembly tank; 12. Testing chamber; 13. Box door; 20. First sprocket; 21. Assembly block; 22. Second sprocket; 23. Chain; 30. Reagent delivery mechanism; 3000. Guide rod; 3001. Limiting block; 3002. Transmission screw; 3003. Driven equal diameter bevel gear; 3004. Guide block; 3005. Mounting tube; 3006. Movable rod; 3007. Support block; 3008. Slot; 3009. Connecting rod; 3010. Bearing block; 3011. Insert; 3012. Bearing groove; 31. Rotating groove; 32. Rotating rod; 33. Rotating block; 40. Lifting mechanism; 4000. Fixed block; 4001. Operating port; 4002. Assembly rod; 4003. Movable block; 4004. Mounting plate; 4005. Vertical rod; 4006. Limiting plate; 4007. Adjusting block; 4008. Fixed plate; 4009. Vertical block; 4010. Insert block; 4011. Traction rod; 4012. Positioning block; 4013. U-shaped slide; 4014. First locking hole; 4015. Second locking hole; 4016. Inner cavity; 4017. Telescopic spring; 4018. Push plate; 4019. Telescopic button; 4020. Adjusting rod; 41. Detection head; 42. Operating motor; 43. Rotating rod; 50. Driven equal-diameter bevel gear; 51. Controller; 52. First connecting line; 53. Second connecting line; 60. Plug. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0018] The following is in conjunction with the appendix Figure 1-6 This application will be described in further detail. This application discloses a rapid nucleic acid detection device for Candida albicans based on Cas12a. (Refer to...) Figure 1 and Figure 2 and Figure 4 as well as Figure 5A rapid nucleic acid detection device for Candida albicans based on Cas12a includes a nucleic acid detector 10. The detector 10 has an assembly slot 11, and a detection chamber 12 is mounted on the assembly slot 11. Multiple detection heads 41 are fixedly mounted on the top surface of the detection chamber 12. A reagent delivery mechanism 30 is mounted on the detection chamber 12. The reagent delivery mechanism 30 includes guide rods 3000. Two guide rods 3000 are fixedly mounted to the inner wall of the detection chamber 12. Limit blocks 3001 are fixedly mounted at one end of each guide rod 3000. Guide blocks 3004 are movably mounted on each guide rod 3000. Installation tubes 3005 are symmetrically fixedly mounted on each guide block 3004. Movable rods 3006 are movably mounted on each installation tube 3005. Support blocks 3007 are fixedly mounted on the top of each of the two movable rods 3006. Slots 3008 are provided on the end faces of the support blocks 3007. Connecting rods 300 are fixedly mounted on the bottom of the support blocks 3007. 9. A bearing block 3010 is fixedly mounted on the bottom of the connecting rod 3009. Multiple insertion ports 3011 are provided on the support block 3007. Multiple bearing grooves 3012 corresponding to and communicating with the insertion ports 3011 are provided on the bearing block 3010. The number of insertion ports 3011 is the same as the number of detection heads 41. A lifting mechanism 40 is mounted on the inner wall of the detection chamber 12. The lifting mechanism 40 includes a fixing block 4000, which is fixed to the inner wall of the detection chamber 12. The fixed assembly includes vertical rods 4005 symmetrically fixedly mounted on the bottom of the fixed block 4000. Limiting plates 4006 are fixedly mounted on the bottom of the two vertical rods 4005. Adjusting blocks 4007 are movably mounted on the two vertical rods 4005. Vertical blocks 4009 are fixedly mounted on the bottom of the adjusting blocks 4007. The vertical blocks 4009 are located on one side of the limiting plates 4006. Insertion blocks 4010 are fixedly mounted on the bottom of the vertical blocks 4009. Insertion blocks 4010 can be inserted into slots 3008.

[0019] In this invention, multiple reagent tubes containing Candida albicans Cas12a are inserted into the sockets 3011 of the support block 3007, so that the bottom of the reagent tubes rests on the support grooves 3012 of the support block 3010. The guide block 3004 moves towards the detection chamber 12 on the two guide rods 3000 until the slot 3008 is inserted into the insertion block 4010. The adjusting block 4007 moves on the two vertical rods 4005, so that the vertical block 4009 drives the insertion block 4010 to rise in height, and the insertion block 4010 drives the support block 3007 to rise in height. One end of the movable rod 3006 moves inside the mounting tube 3005, so that the detection head 41 can be inserted into the Candida albicans Cas12a in the reagent tubes, so that the nucleic acid detector 10 can detect Candida albicans Cas12a through the detection head 41, reducing manual operation and improving detection efficiency.

[0020] Reference Figures 1-4A transmission screw 3002 is movably mounted on the nucleic acid detector 10 and the limiting block 3001. The transmission screw 3002 is threadedly assembled with the guide block 3004. A driven equal-diameter bevel gear 3003 is fixedly mounted on one end of the transmission screw 3002. The driven equal-diameter bevel gear 3003 is located on one side of the nucleic acid detector 10. Assembly blocks 21 are symmetrically fixedly mounted on the back of the nucleic acid detector 10. An operating motor 42 is fixedly mounted on the assembly block 21. A rotating rod 43 is fixedly mounted on the output end of the operating motor 42. A driving equal-diameter bevel gear 50 that is toothed and engaged with the driven equal-diameter bevel gear 3003 is fixedly mounted on the rotating rod 43. A second sprocket 22 is fixedly mounted on one end of the rotating rod 43. The top surface of the assembly groove 11 is provided with... A rotating groove 31 is provided, on which a rotating rod 32 is movably mounted. A rotating block 33 is fixedly mounted on the rotating rod 32. A door 13 is fixedly mounted on the rotating block 33. The door 13 is movably assembled with the assembly groove 11. One end of the rotating rod 32 movably passes through the nucleic acid detector 10, and a first sprocket 20 is fixedly mounted on one end of the rotating rod 32. A chain 23 is wound around the first sprocket 20 and the second sprocket 22. A controller 51 is fixedly mounted on the side of the nucleic acid detector 10. A first connecting line 52 is fixedly mounted on the controller 51. One end of the first connecting line 52 is fixedly assembled with the operating motor 42. A second connecting line 53 is fixedly mounted on the controller 51. A plug 60 is fixedly mounted on one end of the second connecting line 53.

[0021] In this invention, the plug 60 is inserted into the socket. By controlling the controller 51, the operating motor 42 is started. The output end of the operating motor 42 drives the active equal-diameter bevel gear 50 on the rotating rod 43 to rotate. The active equal-diameter bevel gear 50 drives the driven equal-diameter bevel gear 3003 to rotate. The driven equal-diameter bevel gear 3003 drives the transmission screw 3002 to rotate on the guide block 3004, allowing the guide block 3004 to move on the two guide rods 3000. At the same time, the rotating rod 43 drives the second sprocket 22 to rotate. The second sprocket 22 drives the first sprocket 20 to rotate through the chain 23. The first sprocket 20 drives the rotating block 33 on the rotating rod 32 to rotate on the rotating groove 31. The rotating block 33 drives the box door 13 to flip on the assembly groove 11, which can seal the testing chamber 12.

[0022] Reference Figure 3 and Figure 5 as well as Figure 6The fixed block 4000 is provided with an operation port 4001, which is located on one side of the vertical rod 4005. Assembly rods 4002 are symmetrically fixedly mounted on the two operation ports 4001. Movable blocks 4003 are movably mounted on the two assembly rods 4002. A mounting plate 4004 is fixedly mounted on the bottom of the movable block 4003. A fixed plate 4008 is fixedly mounted on the end face of the adjusting block 4007. An adjusting rod 4020 is movably mounted on the fixed plate 4008, with one end of the adjusting rod 4020 movably assembled with the mounting plate 4004. A traction rod 4011 is fixedly mounted on the movable block 4003, movably penetrating the fixed block 4000 and the nucleic acid detector 10. A positioning block 4012 is fixedly mounted on one end of the traction rod 4011, located on one side of the nucleic acid detector 10. The back of the acid detector 10 is fixedly equipped with a U-shaped slide 4013, which is movably assembled with the positioning block 4012. The side of the U-shaped slide 4013 is provided with a first locking hole 4014 and a second locking hole 4015. Both the first locking hole 4014 and the second locking hole 4015 are close to the positioning block 4012. The positioning block 4012 has an inner cavity 4016. A telescopic spring 4017 is fixedly assembled on the inner cavity 4016. A push plate 4018 is fixedly assembled on one end of the telescopic spring 4017. A telescopic button 4019 is fixedly assembled on the push plate 4018. One end of the telescopic button 4019 moves through the positioning block 4012, and one end of the telescopic button 4019 is only inserted into one of the first locking hole 4014 and the second locking hole 4015.

[0023] In this invention, pressing the telescopic button 4019 causes the push plate 4018 to retract the telescopic spring 4017, pushing the positioning block 4012 and causing the traction rod 4011 to move on the nucleic acid detector 10 and the fixing block 4000. The traction rod 4011 pushes the movable block 4003 to move on the two assembly rods 4002. Under the traction of the adjusting rod 4020, the adjusting block 4007 moves on the two vertical rods 4005. At this time, the telescopic button 4019 is released. Under the elastic force of the telescopic spring 4017, one end of the telescopic button 4019 is inserted into only one of the first locking hole 4014 and the second locking hole 4015, increasing the stability of the height adjustment of the insertion block 4010.

[0024] Working principle: Multiple reagent tubes containing Candida albicans Cas12a are inserted into the sockets 3011 of the support block 3007, so that the bottom of the reagent tubes rests on the support grooves 3012 of the support block 3010. The plug 60 is then connected to the socket. By controlling the controller 51, the operating motor 42 is started. The output of the operating motor 42 drives the active equal-diameter bevel gear 50 on the rotating rod 43 to rotate, which in turn drives the driven equal-diameter bevel gear 3003 to rotate. The driven equal-diameter bevel gear 3003 drives the transmission screw 3002 to rotate on the guide block. The guide block 3004 rotates, causing it to move on the two guide rods 3000. Simultaneously, the rotating rod 43 drives the second sprocket 22 to rotate. The second sprocket 22, through the chain 23, drives the first sprocket 20 to rotate. The first sprocket 20 drives the rotating block 33 on the rotating rod 32 to rotate on the rotating groove 31. The rotating block 33 causes the door 13 to flip on the assembly groove 11, sealing the detection chamber 12 until the insertion port 3011 is positioned below the detection head 41. At this point, the insertion block 4010 engages with the slot 3008. Insert the telescopic button 4019 and press it down, causing the push plate 4018 to retract the telescopic spring 4017, pushing the positioning block 4012. This causes the traction rod 4011 to move on the nucleic acid detector 10 and the fixing block 4000. The traction rod 4011 pushes the movable block 4003 to move on the two assembly rods 4002. Under the traction of the adjusting rod 4020, the adjusting block 4007 moves on the two vertical rods 4005. At this time, release the telescopic button 4019, and under the elastic force of the telescopic spring 4017, the... One end of the telescopic button 4019 is inserted into the first locking hole 4014 to increase the stability of the height adjustment of the insert block 4010. The adjusting block 4007 moves on the two vertical rods 4005, causing the vertical block 4009 to drive the insert block 4010 to rise in height. The insert block 4010 drives the support block 3007 to rise in height. One end of the movable rod 3006 moves inside the mounting tube 3005, allowing the detection head 41 to be inserted into the Candida albicans Cas12a in the reagent tube. This allows the nucleic acid detector 10 to detect Candida albicans Cas12a through the detection head 41, reducing manual operation and improving detection efficiency.

[0025] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A rapid nucleic acid detection device for Candida albicans based on Cas12a, characterized in that: include: Nucleic acid testing instrument (10), wherein an assembly slot (11) is provided on the nucleic acid testing instrument (10), a testing chamber (12) is provided on the assembly slot (11), and multiple testing heads (41) are fixedly assembled on the top surface of the testing chamber (12); The reagent delivery mechanism (30) includes guide rods (3000). Two guide rods (3000) are fixedly assembled with the inner wall of the detection chamber (12). Limiting blocks (3001) are fixedly assembled at one end of the two guide rods (3000). Guide blocks (3004) are movably assembled on the two guide rods (3000). Installation tubes (3005) are symmetrically fixedly assembled on the guide blocks (3004). Each installation tube (3005) is movably assembled with a movable part. The movable rod (3006) has a support block (3007) fixedly mounted on the top of the two movable rods (3006). The end face of the support block (3007) is provided with a slot (3008). The bottom of the support block (3007) is fixedly mounted with a connecting rod (3009). The bottom of the connecting rod (3009) is fixedly mounted with a bearing block (3010). The support block (3007) is provided with multiple insertion ports (3011). The bearing block (3010) is provided with multiple bearing grooves (3012). The lifting mechanism (40) includes a fixed block (4000), which is fixedly assembled with the inner wall of the detection chamber (12). Vertical rods (4005) are symmetrically fixedly assembled at the bottom of the fixed block (4000). Limiting plates (4006) are fixedly assembled at the bottom of the two vertical rods (4005). Adjusting blocks (4007) are movably assembled on the two vertical rods (4005). Vertical blocks (4009) are fixedly assembled at the bottom of the adjusting blocks (4007). Vertical blocks (4009) are located on one side of the limiting plates (4006). Insertion blocks (4010) are fixedly assembled at the bottom of the vertical blocks (4009). Insertion blocks (4010) can be inserted into slots (3008).

2. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 1, characterized in that: The bearing groove (3012) is connected to the socket (3011), and the number of sockets (3011) is the same as the number of detection heads (41).

3. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 1, characterized in that: A transmission screw (3002) is movably mounted on the nucleic acid detector (10) and the limiting block (3001). The transmission screw (3002) is threadedly assembled with the guide block (3004). A driven equal diameter bevel gear (3003) is fixedly mounted on one end of the transmission screw (3002). The driven equal diameter bevel gear (3003) is located on one side of the nucleic acid detector (10). An assembly block (21) is symmetrically fixedly mounted on the back of the nucleic acid detector (10). An operating motor (42) is fixedly mounted on the assembly block (21). A rotating rod (43) is fixedly mounted on the output end of the operating motor (42). An active equal diameter bevel gear (50) is fixedly mounted on the rotating rod (43). The active equal diameter bevel gear (50) and the driven equal diameter bevel gear (3003) are threadedly assembled.

4. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 3, characterized in that: One end of the rotating rod (43) is fixedly fitted with a second sprocket (22). The top surface of the assembly groove (11) is provided with a rotating groove (31). A rotating rod (32) is movably fitted on the rotating groove (31). A rotating block (33) is fixedly fitted on the rotating rod (32). A box door (13) is fixedly fitted on the rotating block (33). The box door (13) is movably fitted with the assembly groove (11). One end of the rotating rod (32) movably passes through the nucleic acid detector (10). A first sprocket (20) is fixedly fitted on one end of the rotating rod (32). A chain (23) is wound on the first sprocket (20) and the second sprocket (22).

5. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 3, characterized in that: The nucleic acid detector (10) is fixedly equipped with a controller (51) on its side. A first connecting line (52) is fixedly equipped on the controller (51). One end of the first connecting line (52) is fixedly equipped with an operating motor (42). A second connecting line (53) is fixedly equipped on the controller (51). A plug (60) is fixedly equipped on one end of the second connecting line (53).

6. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 1, characterized in that: The fixed block (4000) is provided with an operating port (4001), which is located on one side of the vertical rod (4005). Two mounting rods (4002) are symmetrically fixedly mounted on the two operating ports (4001), and movable blocks (4003) are movably mounted on the two mounting rods (4002). A mounting plate (4004) is fixedly mounted on the bottom of the movable block (4003), and a fixing plate (4008) is fixedly mounted on the end face of the adjusting block (4007). An adjusting rod (4020) is movably mounted on (4008). One end of the adjusting rod (4020) is movably mounted to the mounting plate (4004). A traction rod (4011) is fixedly mounted on the movable block (4003). The traction rod (4011) movably passes through the fixed block (4000) and the nucleic acid detector (10). A positioning block (4012) is fixedly mounted on one end of the traction rod (4011). The positioning block (4012) is located on one side of the nucleic acid detector (10).

7. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 6, characterized in that: The back of the nucleic acid detector (10) is fixedly equipped with a U-shaped slide (4013). The U-shaped slide (4013) is movably assembled with the positioning block (4012). A first locking hole (4014) is provided on the side of the U-shaped slide (4013), and a second locking hole (4015) is provided on the side of the U-shaped slide (4013). The first locking hole (4014) and the second locking hole (4015) are both close to the positioning block (4012).

8. The rapid nucleic acid detection device for Candida albicans based on Cas12a according to claim 7, characterized in that: The positioning block (4012) has an inner cavity (4016) inside. A telescopic spring (4017) is fixedly mounted on the inner cavity (4016). A push plate (4018) is fixedly mounted on one end of the telescopic spring (4017). A telescopic button (4019) is fixedly mounted on the push plate (4018). One end of the telescopic button (4019) moves through the positioning block (4012), and one end of the telescopic button (4019) is inserted into only one of the first locking hole (4014) and the second locking hole (4015).

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