A double-membrane aeration device for cultivating methanotrophs

Abstract

The utility model relates to biological reactor technical field discloses a kind of double-membrane aeration aerobic methane-oxidizing bacteria culture device.The device includes base, reaction device cavity, water inlet device, water outlet device, methane storage tank, oxygen storage tank, recovery tank, methane aeration membrane assembly and oxygen aeration membrane assembly.Reaction device cavity is installed on base, methane storage tank is connected methane aeration membrane assembly immersed in liquid phase by methane gas pipe, oxygen storage tank is connected oxygen aeration membrane assembly by gas inlet pipe, and two kinds of membrane assembly adopt hollow fiber membrane structure to realize bubble-free diffusion gas supply.The utility model realizes the efficient dissolution of methane and oxygen by double-membrane aeration, combines with circulating filtration system to continuously enrich bacteria body and reuse culture solution, solves the problem that traditional bubble aeration gas utilization rate is low, and bacteria density is limited, with the significant advantages of high mass transfer efficiency, high culture density and low operating cost.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor technology, and in particular to a dual-membrane aeration aerobic methanogenic bacteria cultivation device. Background Technology

[0002] Aerobic methanogenic bacteria are microorganisms that can degrade methane as their sole carbon and energy source, and they have important application value in fields such as coal mine gas treatment, greenhouse gas emission reduction, and biotransformation.

[0003] In the cultivation of aerobic methanogenic bacteria, two key gaseous substrates—methane and oxygen—must be supplied simultaneously to the liquid culture medium. However, both methane and oxygen have very low solubility in aqueous phase, making efficient gas-liquid mass transfer a core bottleneck limiting the growth and metabolic efficiency of the bacteria.

[0004] In existing cultivation techniques, the traditional bubbling aeration method is typically used to supply gas. This method involves introducing gas into the bottom of the culture medium to form bubbles, and dissolution is achieved through diffusion as the bubbles rise.

[0005] However, traditional bubbling aeration produces large bubbles, resulting in limited gas-liquid contact area and low mass transfer efficiency. Most of the undissolved gas escapes directly from the liquid surface, leading to low gas utilization and wasted costs. This inefficient aeration method cannot meet the huge demand for dissolved oxygen and dissolved methane during high-density bacterial growth, severely limiting the improvement of culture efficiency and bacterial concentration.

[0006] Therefore, this invention proposes a dual-membrane aerated aerobic methanogenic bacteria cultivation device to address the shortcomings of existing technologies. Utility Model Content

[0007] In view of the problems in the existing technology, when cultivating aerobic methanogenic bacteria, the traditional aeration method results in low gas-liquid mass transfer efficiency and low gas utilization rate of poorly soluble gases such as methane and oxygen, which in turn limits the culture density and efficiency of bacteria. The present invention aims to provide a dual-membrane aeration aerobic methanogenic bacteria culture device with an improved structure that can effectively solve the above problems.

[0008] This utility model provides a dual-membrane aerated aerobic methanogenic bacteria cultivation device, comprising: a base, a reaction device cavity mounted on the base, a water inlet device and a water outlet device disposed on the reaction device cavity; and a methane storage tank and an oxygen storage tank for gas supply, a methane aeration membrane assembly and an oxygen aeration membrane assembly disposed in the reaction device cavity, a methane gas pipe, an air inlet pipe, a recovery tank, an ultrafiltration membrane assembly and a filter membrane disposed in the recovery tank, a feed pipe, and a return pipe.

[0009] The methane storage tank is connected to the methane aeration membrane assembly via a methane gas pipe, and the oxygen storage tank is connected to the oxygen aeration membrane assembly via an air inlet pipe.

[0010] Furthermore, the recovery tank is connected to the reaction device cavity via a feed pipe, and the recovery tank is also connected to the reaction device cavity via a reflux pipe.

[0011] Preferably, the dual-membrane aerated aerobic methanogenic bacteria culture device further includes a power unit, a rotating rod, and a stirring rod; the power unit is installed at the top of the reaction device cavity, the output end of the power unit is rotatably connected to the rotating rod, and the stirring rod is fixedly connected to the bottom of the rotating rod.

[0012] Preferably, a protective shell is fitted on the outer side of the rotating rod, and the protective shell slides in conjunction with an annular groove on the reaction device cavity, so that the rotating rod and the stirring rod can slide up and down in the vertical direction.

[0013] Preferably, the reaction device cavity is also equipped with an oxygen sensor and a pH sensor.

[0014] Preferably, the feed pipe is used to introduce liquid in the reaction device cavity upstream of the ultrafiltration membrane module and the filter membrane; the return pipe is used to guide liquid back to the reaction device cavity from downstream of the ultrafiltration membrane module and the filter membrane.

[0015] Preferably, the methane aeration membrane module and the oxygen aeration membrane module are hollow fiber membrane modules.

[0016] Preferably, a pump is provided at the end of the conveying pipe to drive the liquid into the recovery tank.

[0017] Preferably, the stirring rod has a multi-layered paddle-type stirring structure.

[0018] This utility model has the following beneficial effects:

[0019] 1. In this utility model, by setting up a methane aeration membrane component and an oxygen aeration membrane component, and connecting them to a methane storage tank and an oxygen storage tank respectively, the two gases enter the reaction device cavity in a bubble-free diffusion manner. This solves the problem of low gas-liquid mass transfer efficiency and low gas utilization rate of poorly soluble gases such as methane and oxygen caused by the use of traditional aeration methods in the prior art. It achieves the technical effect of efficiently providing sufficient substrate for aerobic methane-oxidizing bacteria and significantly improving gas dissolution efficiency and utilization rate.

[0020] 2. In this utility model, by setting up a recovery tank, and the recovery tank is equipped with an ultrafiltration membrane assembly and a filter membrane, and is connected to the reaction device cavity through a feed pipe and a return pipe to form a circulation path, the problem of lack of effective bacterial cell recovery and enrichment mechanism in the prior art, which leads to limited culture density and inability to recycle culture medium, is solved. The technical effect of continuously recovering and enriching bacterial cells during the culture process, realizing high-density culture, and recycling culture medium at the same time saving costs is achieved. Attached Figure Description

[0021] Figure 1 This is a three-dimensional schematic diagram of a dual-membrane aerated aerobic methanogenic bacteria culture device proposed in this utility model;

[0022] Figure 2 This is a schematic diagram of the reaction chamber of a dual-membrane aerated aerobic methanogenic bacteria culture device proposed in this utility model;

[0023] Figure 3 This is a schematic diagram of the structure of the recovery tank of a dual-membrane aerated aerobic methanogenic bacteria culture device proposed in this utility model;

[0024] Figure 4 This is a schematic diagram of the structure of the stirring rod in a dual-membrane aerated aerobic methanogenic bacteria culture device proposed in this utility model.

[0025] Legend: 1. Base; 2. Reaction chamber; 3. Water inlet device; 4. Water outlet device; 5. Methane storage tank; 6. Recovery tank; 7. Oxygen storage tank; 8. Gas inlet pipe; 9. Rotating rod; 10. Methane gas pipe; 11. Oxygen sensor; 12. Feed pipe; 13. pH sensor; 14. Reflux pipe; 15. Ultrafiltration membrane module; 16. Filter membrane; 17. Power unit; 18. Protective shell; 19. Annular groove; 20. Stirring rod. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0027] Example:

[0028] Please refer to Figures 1 to 4This utility model provides a dual-membrane aeration aerobic methanogenic bacteria cultivation device, which aims to solve the problems of low gas-liquid mass transfer efficiency and low gas utilization rate in the traditional aeration method used to supply methane and oxygen and other sparingly soluble gases to the culture medium when cultivating aerobic methanogenic bacteria. At the same time, the lack of an effective bacterial cell recovery and enrichment mechanism leads to the limitation of culture density and production efficiency.

[0029] like Figure 1 and Figure 2 As shown, the dual-membrane aerated aerobic methanogenic bacteria culture device includes a base 1 and a reaction chamber 2 mounted on the base 1. The base 1 provides a stable installation foundation for the entire device, and the reaction chamber 2 provides the main space for the culture reaction. The reaction chamber 2 is equipped with an inlet device 3 and an outlet device 4. The inlet device 3 is used to transport culture medium and inoculum into the reaction chamber 2, and the outlet device 4 is used to discharge the culture liquid in the reaction chamber 2. The device also includes a methane storage tank 5 and an oxygen storage tank 7 for gas supply. The reaction chamber 2 is equipped with a methane aeration membrane assembly and an oxygen aeration membrane assembly, which are hollow fiber membrane assemblies. The methane storage tank 5 is supplied with methane gas. Pipe 10 is connected to the methane aeration membrane assembly, and oxygen storage tank 7 is connected to the oxygen aeration membrane assembly via air inlet pipe 8. An oxygen sensor 11 and a pH sensor 13 are also installed in the reaction device cavity 2. The device also includes a recovery tank 6, which is equipped with an ultrafiltration membrane assembly 15 and a filter membrane 16. The recovery tank 6 is connected to the reaction device cavity 2 via a feed pipe 12. A pump is installed at the end of the feed pipe 12 to drive liquid into the recovery tank 6. The recovery tank 6 is also connected to the reaction device cavity 2 via a return pipe 14. The feed pipe 12 is used to guide the liquid in the reaction device cavity 2 upstream of the ultrafiltration membrane assembly 15 and the filter membrane 16, and the return pipe 14 is used to guide the liquid back to the reaction device cavity 2 from downstream of the ultrafiltration membrane assembly 15 and the filter membrane 16.

[0030] To solve the aforementioned technical problems, the core of the technical solution in this embodiment lies in the fact that the dual-membrane aerated aerobic methanogenic bacteria culture device further includes a power unit 17, a rotating rod 9, and a stirring rod 20. Furthermore, the power unit 17, the rotating rod 9, and the stirring rod 20 form a specific structural fit and connection relationship with the reaction device cavity 2. Please refer to [the relevant documentation / reference]. Figure 1 , Figure 2 and Figure 4The core structure is described in detail below: The power unit 17 is installed on the top of the reaction device cavity 2. The output end of the power unit 17 is rotatably connected to the rotating rod 9. The stirring rod 20 is fixedly connected to the bottom of the rotating rod 9. The stirring rod 20 is a multi-layer paddle-type stirring structure. At the same time, a protective shell 18 is sleeved on the outside of the rotating rod 9. A corresponding annular groove 19 is provided on the reaction device cavity 2. In the assembled state, the protective shell 18 and the annular groove 19 are slidably engaged. This sliding engagement structure between the protective shell and the annular groove ensures that the rotating rod 9 and the stirring rod 20 can slide up and down in the vertical direction.

[0031] Based on the above embodiments, the present invention may further include the following preferred technical solutions:

[0032] As a preferred implementation method, please refer to [the relevant documentation] for real-time monitoring of the culture environment. Figure 2 An oxygen sensor 11 and a pH sensor 13 are also installed in the reaction device chamber 2.

[0033] As a preferred embodiment, please refer to Figure 1 and Figure 3 The feed pipe 12 is used to introduce the liquid in the reaction device chamber 2 upstream of the ultrafiltration membrane assembly 15 and the filter membrane 16, and the return pipe 14 is used to guide the filtered liquid back to the reaction device chamber 2 from the downstream of the ultrafiltration membrane assembly 15 and the filter membrane 16.

[0034] As a preferred embodiment, in order to improve gas-liquid mass transfer efficiency, the methane aeration membrane module and the oxygen aeration membrane module are preferably hollow fiber membrane modules.

[0035] As a preferred embodiment, in order to provide liquid circulation power, please refer to... Figure 1 A pump is installed at the end of the feed pipe 12 to drive the liquid from the reaction device chamber 2 into the recovery tank 6.

[0036] Working principle: When the device is running, the methanogenic bacteria inoculum and culture medium are transported to the reaction chamber 2 through the water inlet device 3. The power device 17 is started and the rotating rod 9 connected to its output end rotates. The rotating rod 9 drives the stirring rod 20 fixedly connected to its bottom to stir the liquid in the reaction chamber 2. At the same time, methane enters the methane aeration membrane component from the methane storage tank 5 through the methane gas pipe 10, and oxygen enters the oxygen aeration membrane component from the oxygen storage tank 7 through the air inlet pipe 8. Both gases enter the liquid phase in a bubble-free diffusion manner. The stirring of the stirring rod 20 makes the substrate and microorganisms evenly mixed. The protective shell 18 sleeved on the outside of the rotating rod 9 slides with the annular groove 19, so that the rotating rod 9 and the stirring rod 20 can slide up and down in the vertical direction.

[0037] During the cultivation process, oxygen sensor 11 and pH sensor 13 monitor the environmental parameters inside the reaction device chamber 2 in real time. Part of the reaction liquid is driven by a pump installed at the end of the feed pipe 12 and enters the recovery tank 6 through the feed pipe 12. In the recovery tank 6, the liquid passes through the ultrafiltration membrane assembly 15 and the filter membrane 16. The filtered liquid is then returned to the reaction device chamber 2 through the return pipe 14, realizing the recovery of cells or products and the recycling of reaction liquid.

[0038] By using a bubble-free gas supply method with methane aeration membrane components and oxygen aeration membrane components, and a circulating filtration system with recovery tank 6, ultrafiltration membrane component 15, and filter membrane 16, this invention solves the problems of low gas mass transfer efficiency and difficulty in enriching bacterial cells, resulting in low culture density in the prior art.

[0039] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A dual-membrane aerated aerobic methanogenic bacteria cultivation device, comprising: A base (1), and a reaction device cavity (2) mounted on the base (1); The reaction device cavity (2) is equipped with a water inlet device (3) and a water outlet device (4). Its features are, The device also includes a methane storage tank (5) and an oxygen storage tank (7) for gas supply. The reaction device cavity (2) is equipped with a methane aeration membrane assembly and an oxygen aeration membrane assembly; The methane storage tank (5) is connected to the methane aeration membrane assembly via a methane gas pipe (10), and the oxygen storage tank (7) is connected to the oxygen aeration membrane assembly via an air inlet pipe (8). The device also includes a recovery tank (6), in which an ultrafiltration membrane assembly (15) and a filter membrane (16) are disposed. The recovery tank (6) is connected to the reaction device cavity (2) through the feed pipe (12), and the recovery tank (6) is also connected to the reaction device cavity (2) through the return pipe (14).

2. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 1, characterized in that, The device also includes a power unit (17), a rotating rod (9), and a stirring rod (20); the power unit (17) is installed on the top of the reaction device cavity (2), the output end of the power unit (17) is rotatably connected to the rotating rod (9), and the stirring rod (20) is fixedly connected to the bottom of the rotating rod (9).

3. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 2, characterized in that, The outer side of the rotating rod (9) is fitted with a protective shell (18), which slides in conjunction with the annular groove (19) on the reaction device cavity (2), so that the rotating rod (9) and the stirring rod (20) can slide up and down in the vertical direction.

4. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 1, characterized in that, The reaction device cavity (2) is also equipped with an oxygen sensor (11) and a pH sensor (13).

5. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 1, characterized in that, The feed pipe (12) is used to introduce the liquid in the reaction device cavity (2) upstream of the ultrafiltration membrane assembly (15) and the filter membrane (16), and the return pipe (14) is used to draw the liquid back to the reaction device cavity (2) from the downstream of the ultrafiltration membrane assembly (15) and the filter membrane (16).

6. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 1, characterized in that, The methane aeration membrane module and the oxygen aeration membrane module are hollow fiber membrane modules.

7. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 1, characterized in that, A pump is provided at the end of the conveying pipe (12) to drive the liquid into the recovery tank (6).

8. The dual-membrane aerated aerobic methanogenic bacteria cultivation device according to claim 2, characterized in that, The stirring rod (20) has a multi-layer paddle-type stirring structure.

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