Bacterial and algal culture detection device
Patent Information
- Application Number
- CN202522105104.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2026-09-25
- Estimated Expiration
- 2035-09-29
AI Technical Summary
[0004]有鉴于此，本实用新型的实施例提供了菌藻培养检测装置，用于解决现有的微生物培养监测整合费时费力以及忽略藻菌共生干扰的技术问题
[0013]本实用新型的实施例提供的技术方案带来的有益效果是：本实用新型的菌藻培养检测装置，通过“沉淀-多级菌藻生化-全参数闭环控制”一体化设计，将静压液位、浊度、溶氧温度、pH、氨氮、硝酸盐、总氮总磷等传感器阵列与西门子PLC、变频器、喷淋泵、提升泵深度耦合，实现数据采集、指令响应和周期自动校准，在进水TN 400 mg/L、TP 50 mg/L条件下连续运行90天，TN去除率75%~80%、TP去除率82%~85%，电耗0.5 kWh/m3、碳源零投加，pH波动由±1.2降至±0.3，冬季<10 ℃时TP去除率仍>82%，模块化规模10-3000 m3/d可扩展，显著优于传统活性污泥法，兼具节能降耗、运行稳定、维护简易、应用面广的综合优势。
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Figure CN224798893U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microbial cultivation and monitoring technology, and in particular to a device for detecting bacterial and algal cultivation. Background Technology
[0002] Currently, in applications of microbial cultivation and water quality monitoring, the monitoring of key water quality indicators such as dissolved oxygen, pH, and nitrate still generally adopts a "single-parameter independent device" model: a dissolved oxygen meter is responsible for dissolved oxygen measurement, a pH meter for acidity / alkalinity detection, and an ion-selective electrode or spectrophotometer for nitrate concentration analysis. Each instrument is independently deployed and outputs data independently. The sampling process is usually "on-site sampling—laboratory analysis—manual summarization." The entire system relies on hardware from multiple brands and offline data post-processing, forming a relatively fixed technical path.
[0003] However, the aforementioned "multi-machine discrete + offline analysis" model has increasingly revealed two shortcomings: First, feedback is delayed, as water sample transportation and experimental analysis often take several hours or even days, making it impossible to capture transient changes in algal blooms or imbalances in the bacterial-algal symbiotic system; second, biological interference is ignored, as traditional sensors treat microorganisms as "background noise" and neglect the dynamic coupling effect of bacterial-algal synergistic metabolism on indicators such as dissolved oxygen, pH, and nitrate, resulting in significant deviations between monitoring results and actual ecological processes, making it difficult to support precise regulation. Utility Model Content
[0004] In view of this, embodiments of the present invention provide a bacterial and algal culture detection device to solve the technical problems of existing microbial culture monitoring and integration being time-consuming and labor-intensive, and neglecting the interference of algal symbiosis.
[0005] Embodiments of this utility model provide a bacterial and algal culture detection device, comprising: Main control cabinet; A sedimentation unit includes a sedimentation tank, a sedimentation tank lift pump, a sedimentation tank sensor, and a first sub-control cabinet. The sedimentation tank sensor is built into the sedimentation tank, and the first sub-control cabinet is connected to the sedimentation tank sensor and the main control cabinet via cables. Multiple biochemical units are provided, each of which includes a biochemical tank, a biochemical tank lift pump, a biochemical tank spray pump, a data acquisition unit, a second sub-control cabinet, and a sensor array. The data acquisition unit, the sensor array, and the second sub-control cabinet are connected by cables, and the multiple second sub-control cabinets are electrically connected to the main control cabinet. Each of the biochemical tanks has an inlet on one side wall and an outlet on the other side wall. The inlet and outlet between two adjacent biochemical units are connected by a water pipe through a biochemical tank booster pump. The sensor array includes a liquid level sensor, a dissolved oxygen temperature sensor, a pH sensor, an ammonia nitrogen sensor, a nitrate sensor, a turbidity sensor, a total nitrogen sensor, and a total phosphorus sensor. The ammonia nitrogen sensor and the turbidity sensor are located at the inlet, the total nitrogen sensor and the total phosphorus sensor are located at the outlet, and the dissolved oxygen temperature sensor, the pH sensor, and the nitrate sensor are located in the middle of the biological treatment tank, for the purpose of integrated acquisition of effluent water quality indicators.
[0006] Furthermore, the sedimentation tank sensor includes a level sensor and a flow sensor, both of which are linked to the sedimentation tank lift pump so that the start and stop values of the sedimentation tank lift pump can be set.
[0007] Furthermore, a sedimentation tank outlet is provided on one side wall of the sedimentation tank, and the sedimentation tank lift pump is connected to each of the biological tanks through a water pipe, so that the ratio of bacteria and algae in each of the biological tanks can be adjusted.
[0008] Furthermore, the second sub-control cabinet is electrically connected to the biochemical pool booster pump and the biochemical pool spray pump for remote control of equipment start-up and shutdown.
[0009] Furthermore, the inlet and outlet between two adjacent biochemical units are connected by a water pipe via a biochemical pool booster pump.
[0010] Furthermore, a biofilm is provided in both the sedimentation tank and each of the biochemical tanks.
[0011] Furthermore, the main control cabinet is equipped with a PLC interaction module, and the human-machine interaction platform controls the first sub-control cabinet and multiple second sub-control cabinets through the PLC interaction module.
[0012] Furthermore, each of the biochemical units also includes a biochemical tank spray pump, which is used for aeration and oxygenation.
[0013] The beneficial effects of the technical solution provided by the embodiments of this utility model are as follows: The bacterial and algal culture detection device of this utility model, through the integrated design of "sedimentation-multi-level bacterial and algal biochemistry-full parameter closed-loop control", deeply couples the sensor arrays of static pressure level, turbidity, dissolved oxygen temperature, pH, ammonia nitrogen, nitrate, total nitrogen and total phosphorus with Siemens PLC, frequency converter, spray pump and lift pump to realize data acquisition, command response and periodic automatic calibration. Under the condition of influent TN 400 mg / L and TP 50 mg / L, it can operate continuously for 90 days with a TN removal rate of 75%~80% and a TP removal rate of 82%~85%, and the power consumption is 0.5 kWh / m³. 3Zero carbon source addition; pH fluctuation reduced from ±1.2 to ±0.3; TP removal rate still >82% at winter temperatures <10 ℃; modular scale 10-3000 m³ 3 / d is scalable and significantly superior to the traditional activated sludge process, while also possessing comprehensive advantages such as energy saving, stable operation, easy maintenance, and wide application. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the structure of the bacterial and algal culture and detection device of this utility model; Figure 2 This is a schematic diagram of the sedimentation unit structure of the bacterial and algal culture and detection device of this utility model.
[0015] In the diagram: 1. Main control cabinet; 11. PLC interactive module; 2. Sedimentation unit; 21. Sedimentation tank; 22. First sub-control cabinet; 23. Sedimentation tank sensor; 24. Sedimentation tank outlet; 25. Sedimentation tank lift pump; 3. Biochemical unit; 31. Biochemical tank; 32. Biochemical tank lift pump; 33. Biochemical tank spray pump; 34. Data acquisition unit; 35. Second sub-control cabinet; 36. Sensor array; 361. Ammonia nitrogen sensor; 362. Turbidity sensor; 363. Dissolved oxygen temperature sensor; 364. pH sensor; 365. Total nitrogen sensor; 366. Total phosphorus sensor; 367. Nitrate sensor; 4. Water pipe. Detailed Implementation
[0016] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be further described below with reference to the accompanying drawings. The following description presents a preferred embodiment of several possible embodiments of this utility model, intended to provide a basic understanding of the utility model, but not intended to identify the key or decisive elements of the utility model or to limit the scope of protection sought.
[0017] In all examples shown and discussed herein, any specific values should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values.
[0018] Techniques, methods, and equipment known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and equipment should be considered part of the specification.
[0019] It should be noted that similar labels and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be discussed further in subsequent figures. Also, it should be understood that, for ease of description, the dimensions of the various parts shown in the figures are not drawn to actual scale.
[0020] In the description of this utility model, it should be noted that the circuits, electronic components and modules involved in this utility model are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated. The content protected by this utility model does not involve any improvement to the internal structure and method.
[0021] It should be further noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0022] Please refer to Figures 1 to 2 The present invention provides a bacterial and algal culture detection device, which includes a main control cabinet 1, a sedimentation unit 2 and multiple biochemical units 3 integrated in one unit; the main control cabinet 1 uses an industrial touch screen and a Siemens PLC interactive module 11 as the core controller, and is connected to the sedimentation unit 2 and multiple biochemical units 3 via an industrial Ethernet switch to realize the construction of a control system and complete parameter setting, real-time curve display, alarm log archiving and remote diagnosis.
[0023] The sedimentation unit 2 includes a sedimentation tank 21, a sedimentation tank lift pump 25, a sedimentation tank sensor 23, and a first sub-control cabinet 22. The sedimentation tank sensor 23 includes a level sensor and a flow sensor. The sedimentation tank 21 is a rectangular reinforced concrete tank with an effective volume of 2 m³. 3 The top has a pre-installed φ110 mm sensor mounting sleeve, and the bottom suspends a Ø150 mm combined elastic three-dimensional packing with a specific surface area ≥250 m². 2 / m 3 It is used to enrich denitrifying bacteria. A hydrostatic level sensor (0-65 mH2O, ±0.1% F·S) and an electromagnetic flow sensor (0-20 m) are installed in the tank. 3 / h, ±1%F·S, both signals are connected to the AI module in the first sub-control cabinet 22. The PLC uses the PID algorithm to link the liquid level signal with the sedimentation tank lift pump 25. The start and stop thresholds can be set arbitrarily within the 0.1 m accuracy of the touch screen to achieve dual protection against dry pumping and overflow.
[0024] In this embodiment, the sedimentation tank lift pump 25 is a submersible sewage pump with a rated flow rate of 5 m³ / h. 3 / h, a sedimentation tank outlet 24 is provided on one side wall of the sedimentation tank 21. The sedimentation tank outlet 24 is connected in parallel to the inlet water distribution pipe 4 of each stage of the biological treatment tank 31 via a DN50 UPVC pipe and an electric ball valve, and the flow rate can be flexibly allocated according to the real-time bacteria and algae ratio requirements.
[0025] It is understandable that the first sub-control cabinet 22 of the sedimentation unit 2 and the second sub-control cabinet 35 of each biochemical unit 3 are electrically connected to the main control cabinet 1.
[0026] Multiple biochemical units 3 are connected in parallel. Each biochemical unit 3 includes a biochemical tank 31, a biochemical tank booster pump 32, a biochemical tank spray pump 33, a data acquisition unit 34, a second sub-control cabinet 35, and a sensor array 36. The effective volume of the biochemical tank 31 is 1.5 m³. 3 The same elastic packing material as sedimentation tank 21 is suspended in the pool to form a biofilm carrier for bacterial and algal symbiosis.
[0027] It should be noted that the sensor array 36 includes a liquid level sensor, a dissolved oxygen temperature sensor 363, a pH sensor 364, an ammonia nitrogen sensor 361, a nitrate sensor 367, a turbidity sensor 362, a total nitrogen sensor 365, and a total phosphorus sensor 366. Each sensor corresponds to a key technical indicator, which is shown in the table below:
[0028] Each of the aforementioned biological treatment tanks 31 has an inlet 311 on one side wall and an outlet 312 on the other side wall. To form a differentiated monitoring system of "inlet-middle-outlet", each biological treatment tank 31 is equipped with a sensor array as follows: an ammonia nitrogen sensor 361 and a turbidity sensor 362 are installed at the inlet 311 to capture the influent shock load; a dissolved oxygen temperature sensor 363, a pH sensor 364, and a nitrate sensor 367 are installed in the middle of the biological treatment tank 31 to reflect the metabolic activity of bacteria and algae in real time; a total nitrogen sensor 365 and a total phosphorus sensor 366 are installed at the outlet to evaluate the eutrophication risk of the effluent; all sensors are connected to a data acquisition unit 34 via the RS-485 Modbus-RTU protocol, and the data acquisition unit 34 then uploads the data to the second sub-control cabinet 35 via industrial Ethernet, which in turn uploads it to the main control cabinet 1, forming a "two-layer, three-level" communication network. The data refresh cycle is ≤2 s, and the control command delay is ≤500ms.
[0029] In an optional embodiment, the second sub-control cabinet 35 has two built-in frequency converters to drive the biological tank lift pump 32 and the biological tank spray pump 33 respectively. The PLC automatically adjusts the speed of the spray pump through closed-loop control based on the real-time values of dissolved oxygen and ammonia nitrogen in the middle, maintaining the optimal bacterial-algae symbiotic range of DO 2-4 mg / L. The biological tank lift pump 32 is interlocked by the liquid level sensor to realize low liquid level shutdown protection. In this embodiment, the control system automatically pops up a calibration reminder every Monday at 02:00. After manual confirmation on the touchscreen, the PLC opens the bypass solenoid valve to perform two-point calibration. Biofilm activity is manually examined under a microscope once a week, and the results are written back to the PLC to automatically generate an activity curve. When any sensor reading exceeds the set threshold, such as pH < 6.5 or > 8.5, DO < 1 mg / L, or ammonia nitrogen > 120% of the set load, the PLC triggers a three-level alarm: HMI pop-up window + audible and visual alarm, 4G module SMS push, and data platform WeChat mini-program alarm.
[0030] The removal rates of nitrate and total nitrogen in the effluent for specific embodiments are shown in the table below:
[0031] Traditional systems achieve a total nitrogen removal rate of 60%, while this system increases the total nitrogen removal rate to 75%~80%.
[0032] In this document, the directional terms such as front, back, top, and bottom are defined based on the position of the components in the accompanying drawings and their relative positions to each other, solely for the purpose of clarity and convenience in expressing the technical solution. It should be understood that these are relative concepts and can vary depending on different methods of use and placement; the use of these directional terms should not limit the scope of protection claimed in this application.
[0033] Where there is no conflict, the above embodiments and features described herein can be combined with each other.
[0034] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A bacterial and algal culture and detection device, characterized in that, include: Main control cabinet (1); The sedimentation unit (2) includes a sedimentation tank (21), a sedimentation tank lift pump (25), a sedimentation tank sensor (23), and a first sub-control cabinet (22). The sedimentation tank sensor (23) is built into the sedimentation tank (21), and the first sub-control cabinet (22) is connected to the sedimentation tank sensor (23) and the main control cabinet (1) via cables. Multiple biochemical units (3), each of the biochemical units (3) includes a biochemical tank (31), a biochemical tank booster pump (32), a data acquisition unit (34), a second sub-control cabinet (35) and a sensor array (36). The data acquisition unit (34), the sensor array (36) and the second sub-control cabinet (35) are connected by cables. Multiple second sub-control cabinets (35) are electrically connected to the main control cabinet (1). Each of the biochemical pools (31) has an inlet (311) on one side wall and an outlet (312) on the other side wall. The sensor array (36) includes a liquid level sensor, a dissolved oxygen temperature sensor (363), a pH sensor (364), an ammonia nitrogen sensor (361), a nitrate sensor (367), a turbidity sensor (362), a total nitrogen sensor (365), and a total phosphorus sensor (366). The ammonia nitrogen sensor (361) and the turbidity sensor (362) are located at the inlet (311), the total nitrogen sensor (365) and the total phosphorus sensor (366) are located at the outlet (312), and the dissolved oxygen temperature sensor (363), the pH sensor (364), and the nitrate sensor (367) are located in the middle of the biological treatment tank (31) for integrated acquisition of effluent water quality indicators.
2. The bacterial and algal culture and detection device as described in claim 1, characterized in that: The sedimentation tank sensor (23) includes a liquid level sensor and a flow sensor. Both the liquid level sensor and the flow sensor are linked to the sedimentation tank lift pump (25) so that the start and stop values of the sedimentation tank lift pump (25) can be set.
3. The bacterial and algal culture and detection device as described in claim 1, characterized in that: The sedimentation tank (21) has a sedimentation tank outlet (24) on one side wall. The sedimentation tank lift pump (25) is connected to each of the biochemical tanks (31) through a water pipe (4) so that the ratio of bacteria and algae in each of the biochemical tanks (31) can be adjusted.
4. The bacterial and algal culture and detection device as described in claim 1, characterized in that: The inlet (311) and outlet (312) between two adjacent biochemical units (3) are connected to a water pipe (4) via a biochemical pool booster pump (32).
5. The bacterial and algal culture and detection device as described in claim 1, characterized in that: Each sedimentation tank (21) and each of the biochemical tanks (31) is equipped with a biofilm.
6. The bacterial and algal culture and detection device as described in claim 1, characterized in that: The main control cabinet (1) is equipped with a PLC interaction module (11), and the human-machine interaction platform controls the first sub-control cabinet (22) and multiple second sub-control cabinets (35) through the PLC interaction module (11).
7. The bacterial and algal culture and detection device as described in claim 1, characterized in that: Each of the biochemical units (3) also includes a biochemical tank spray pump (33) for aeration and oxygenation.
8. The bacterial and algal culture detection device as described in claim 7, characterized in that: The second sub-control cabinet (35) is electrically connected to the biochemical pool booster pump (32) and the biochemical pool spray pump (33) for remote control of equipment start and stop.