An integrated hollow fiber membrane biocatalytic reaction device

Abstract

The utility model discloses an integrated hollow fiber membrane biological catalysis reaction device, including reaction column main part, substrate delivery part, constant temperature water bath jacket and collection department: the inside of this reaction column main part is provided with enzyme carrier filling, this substrate delivery part sets up in the side of reaction column main part, this double plunger feed pump sets up in the double plunger feed pump of reaction column main part left side below, this double plunger feed pump is connected with substrate mixing jar, this raw material storage jar is connected with double plunger feed pump, this raw material storage jar is connected with reaction column main part, this constant temperature water bath jacket is wrapped in the outside of reaction column main part, this collection department sets up in the side of reaction column main part. The utility model discloses through being provided with reaction column main part, can realize efficient, stable biological catalysis reaction, and reaction column main part provides the reaction place, and substrate delivery part accurately controls substrate feed, and constant temperature water bath jacket maintains reaction temperature, and collection department real -time monitoring and collecting product to improve reaction efficiency.

Description

Technical Field

[0001] This utility model relates to the field of liquor brewing technology, specifically to an integrated hollow fiber membrane biocatalytic reaction device. Background Technology

[0002] Ethyl hexanoate is an important food flavoring agent, widely used in baijiu (Chinese liquor), beverages, and confectionery. In strong-aroma baijiu, it is the core aroma component that determines the "rich cellar aroma" characteristic, and its typical fruity and cellar-based aromas are crucial to the flavor and quality of the baijiu.

[0003] Traditional chemical catalysis methods, while fast, suffer from numerous side reactions, low product purity, and severe equipment corrosion. Enzymatic catalysis, on the other hand, is mild and environmentally friendly, but practical applications still face challenges such as limited enzyme immobilization efficiency, complex control of reaction temperature and substrate ratios, and long reaction cycles, leaving room for improvement in production efficiency and stability. Therefore, there is an urgent need for an efficient, easily controllable, and readily available integrated biocatalytic reaction device for immobilized enzymes to enhance the synthesis efficiency and product consistency of ethyl hexanoate. Utility Model Content

[0004] The purpose of this invention is to provide an integrated hollow fiber membrane biocatalytic reaction device to solve the problems mentioned in the background art.

[0005] To address the above problems, this utility model provides the following technical solution: an integrated hollow fiber membrane biocatalytic reaction device, comprising a reaction column body, a substrate delivery section, a constant temperature water bath jacket, and a collection section.

[0006] The interior of the reaction column is filled with enzyme carrier. The substrate delivery unit is located on the side of the reaction column and includes a dual-plunger feed pump, a substrate mixing tank, and a raw material storage tank. The dual-plunger feed pump is located on the lower left side of the reaction column and is connected to the substrate mixing tank. The raw material storage tank is connected to the dual-plunger feed pump and to the reaction column. A constant temperature water bath jacket surrounds the outside of the reaction column. A collection unit is located on the side of the reaction column to collect the products after the reaction inside the column.

[0007] By adopting the above technical solution, efficient and stable biocatalytic reactions can be achieved. The main body of the reaction column provides the reaction site, the substrate delivery unit precisely controls the substrate feed, the constant temperature water bath jacket maintains the reaction temperature, and the collection unit monitors and collects the products in real time, thereby improving the reaction efficiency.

[0008] Preferably, the enzyme carrier is filled with a hollow fiber membrane bundle, which is tightly filled inside the reaction column body. The enzyme carrier is made of polyvinylidene fluoride, with an inner diameter of 0.5 mm, an outer diameter of 1.2 mm, and a length of 900 mm. The two ends of the hollow fiber membrane bundle are fixed to the inner wall of the reaction column body with epoxy resin to form a sealed reaction chamber.

[0009] By adopting the above technical solutions, the enzyme immobilization efficiency can be improved. The high specific surface area of ​​the hollow fiber membrane bundle can enhance the enzyme loading capacity. At the same time, the epoxy resin seal ensures the airtightness of the reaction chamber and prevents substrate or product leakage.

[0010] Preferably, the specific surface area of ​​the hollow fiber membrane filled in the enzyme carrier reaches 1000 m² / m³, and the enzyme immobilization capacity is 5 times that of traditional particulate carriers.

[0011] By adopting the above technical solutions, enzyme catalytic efficiency can be improved. The high specific surface area of ​​the hollow fiber membrane bundles greatly increases the enzyme loading, thereby accelerating the reaction rate and improving the overall reaction efficiency.

[0012] Preferably, the reaction column body has an enzyme solution injection port in the middle of the column body, through which the enzyme solution is injected into the enzyme carrier filling.

[0013] By adopting the above technical solution, enzyme immobilization can be performed conveniently and quickly. The enzyme solution injection port design ensures that the enzyme solution is evenly distributed within the hollow fiber membrane bundle, improving the uniformity and stability of enzyme loading.

[0014] Preferably, there are two raw material storage tanks, each storing hexanoic acid and ethanol respectively. Each raw material storage tank is individually connected to a dual-plunger feed pump, and the two dual-plunger feed pumps are connected to the same substrate mixing tank.

[0015] By adopting the above technical solution, the substrate ratio can be precisely controlled, and the dual-plunger feed pump can independently adjust the feed rate of hexanoic acid and ethanol, ensuring that the substrate is uniformly mixed in the substrate mixing tank and improving the controllability of the reaction.

[0016] Preferably, the constant temperature water bath jacket has an inlet on the bottom left side and an outlet on the top right side, and the constant temperature water bath jacket is connected to the constant temperature water tank through a circulating water pump.

[0017] By adopting the above technical solution, the reaction temperature can be precisely controlled. The circulating water system of the constant temperature water bath jacket ensures that the internal temperature of the reaction column is uniform and stable, which is conducive to the efficient progress of enzyme catalysis reaction.

[0018] Preferably, the collection section also has a product collection tank located on the side of the reaction column body. An online refractometer is installed at the top inlet of the product collection tank and connected to the product outlet of the reaction column body. A central control panel is installed on the side of the product collection tank. The online refractometer monitors the concentration of ethyl hexanoate in real time and transmits the data to the central control panel.

[0019] By adopting the above technical solution, real-time monitoring and automated control can be achieved. The online refractometer can detect the product concentration in real time and transmit the data to the central control console, which facilitates the adjustment of reaction parameters and improves the accuracy and automation of the reaction.

[0020] Compared with existing technologies, the advantages of this invention are as follows: By setting up a reaction column body, a highly efficient and stable biocatalytic reaction can be achieved. The reaction column body provides the reaction site, the substrate delivery unit precisely controls the substrate feed, the constant temperature water bath jacket maintains the reaction temperature, and the collection unit monitors and collects the products in real time, thereby improving the reaction efficiency. Hollow fiber membranes are used as enzyme immobilization carriers with a specific surface area of ​​1000 m² / m³, and the enzyme immobilization amount is 5 times that of traditional particulate carriers, increasing the ethyl hexanoate synthesis efficiency by 20%. A "lipase-ester synthase" tandem reaction system is constructed, completing the two-step reaction of "fatty acid esterification-ester synthesis" in the same reaction column, eliminating the intermediate product purification process. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this application;

[0022] Figure 2 This is a schematic diagram of the main structure of the reaction column in this application;

[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of the reaction column in this application;

[0024] Figure 4 This is a schematic cross-sectional view of the connection between the reaction column body and the constant temperature water bath jacket in this application.

[0025] In the diagram: 1. Reaction column body; 101. Enzyme carrier filling; 2. Substrate delivery unit; 201. Dual plunger feed pump; 202. Substrate mixing tank; 203. Raw material storage tank; 3. Constant temperature water bath jacket; 4. Collection unit; 401. Product collection tank; 402. Online refractometer; 403. Central control panel. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Example 1

[0028] Please see Figure 1 , Figure 2 and Figure 3 This embodiment provides a technical solution: an integrated hollow fiber membrane biocatalytic reaction device, comprising a reaction column body 1, a substrate delivery unit 2, a constant temperature water bath jacket 3, and a collection unit 4.

[0029] The reaction column body 1 is made of 304 stainless steel, cylindrical in shape, with an inner diameter of 50mm, a total height of 1000mm, and a wall thickness of 5mm. Flanges are provided at both ends of the column body, with a flange diameter of 100mm and a thickness of 10mm, and are sealed to the end caps of the reaction column body 1 by bolts. The interior of the reaction column body 1 contains an enzyme carrier filling 101. The substrate delivery unit 2 is located on the side of the reaction column body 1. The substrate delivery unit 2 includes a dual-plunger feed pump 201, a substrate mixing tank 202, and a raw material storage tank 203. The dual-plunger feed pump 201 is located on the lower left side of the reaction column body 1. The plunger feed pump 201 is connected to the substrate mixing tank 202, the raw material storage tank 203 is connected to the dual plunger feed pump 201, the raw material storage tank 203 is connected to the reaction column body 1, the constant temperature water bath jacket 3 is wrapped around the outside of the reaction column body 1, and the collection part 4 is set on the side of the reaction column body 1 to collect the products after the reaction inside the reaction column body 1. This can realize efficient and stable biocatalytic reaction. The reaction column body 1 provides the reaction site, the substrate delivery part 2 precisely controls the substrate feed, the constant temperature water bath jacket 3 maintains the reaction temperature, and the collection part 4 monitors and collects the products in real time, thereby improving the reaction efficiency.

[0030] Example 2

[0031] Please see Figure 2 , Figure 3 and Figure 4 This embodiment provides a technical solution: an integrated hollow fiber membrane biocatalytic reaction device, comprising a reaction column body 1, a substrate delivery unit 2, a constant temperature water bath jacket 3, and a collection unit 4.

[0032] The enzyme carrier filler 101 is a hollow fiber membrane bundle, tightly packed inside the reaction column body 1. It is made of polyvinylidene fluoride (PVDF), with an inner diameter of 0.5 mm, an outer diameter of 1.2 mm, and a length of 900 mm. The two ends of the hollow fiber membrane bundle are fixed to the inner wall of the reaction column body 1 with epoxy resin, forming a sealed reaction chamber. This improves enzyme immobilization efficiency, and the high specific surface area of ​​the hollow fiber membrane bundle enhances enzyme loading capacity. Simultaneously, the epoxy resin seal ensures the airtightness of the reaction chamber, preventing substrate or product leakage.

[0033] The specific surface area of ​​the hollow fiber membrane filled in 101 is 1000 m² / m³, and the enzyme immobilization capacity is 5 times that of traditional particulate carriers, which can improve enzyme catalytic efficiency. The high specific surface area of ​​the hollow fiber membrane bundle greatly increases the enzyme loading capacity, thereby accelerating the reaction rate and improving the overall reaction efficiency.

[0034] The reaction column body 1 has an enzyme solution injection port in the middle. Lipase and ester synthase are mixed at a mass ratio of 1:1 and then injected under pressure into the hollow fiber membrane pores through the enzyme solution injection port in the middle of the reaction column body 1 to complete the immobilization. This allows for convenient and quick enzyme immobilization. The design of the enzyme solution injection port ensures that the enzyme solution is evenly distributed in the hollow fiber membrane bundle, improving the uniformity and stability of the enzyme load.

[0035] There are two raw material storage tanks 203, which store hexanoic acid and ethanol respectively. Each raw material storage tank 203 is individually connected to a dual-plunger feed pump 201. The two dual-plunger feed pumps 201 are connected to the same substrate mixing tank 202, which can precisely control the substrate ratio. The dual-plunger feed pumps 201 can independently adjust the feed rate of hexanoic acid and ethanol to ensure that the substrate is uniformly mixed in the substrate mixing tank 202 and improve the controllability of the reaction.

[0036] The constant temperature water bath jacket 3 has an inlet on the bottom left and an outlet on the top right. The constant temperature water bath jacket 3 is connected to the constant temperature water bath through a circulating water pump, which can precisely control the reaction temperature. The circulating water system of the constant temperature water bath jacket 3 ensures that the internal temperature of the reaction column body 1 is uniform and stable, which is conducive to the efficient progress of enzyme catalysis reaction.

[0037] The collection unit 4 also has a product collection tank 401 located on the side of the reaction column body 1. An online refractometer 402 is installed at the top inlet of the product collection tank 401 and connected to the product outlet of the reaction column body 1. A central control panel 403 is installed on the side of the product collection tank 401. The online refractometer 402 monitors the concentration of ethyl hexanoate in real time and transmits the data to the central control panel 403, which can realize real-time monitoring and automated control. The online refractometer 402 detects the product concentration in real time and transmits the data to the central control panel 403, which facilitates the adjustment of reaction parameters and improves the accuracy and automation of the reaction.

[0038] Working principle: First, hexanoic acid and ethanol stored in raw material storage tank 203 are respectively transported to substrate mixing tank 202 through a separately connected dual-plunger feed pump 201. The dual-plunger feed pump 201 can precisely control the feed rate of hexanoic acid and ethanol, thereby precisely controlling the substrate ratio and ensuring that the two substrates are uniformly mixed in substrate mixing tank 202. The mixed substrate enters the reaction column body 1, which is tightly filled with hollow fiber membrane bundles as enzyme carriers 101. At the enzyme inlet in the middle of the reaction column body 1, lipase and ester synthase, which are pre-mixed at a mass ratio of 1:1, are pressurized and injected into the pores of the hollow fiber membrane to complete immobilization. Since the specific surface area of ​​the hollow fiber membrane bundle is 1000m² / m³, the enzyme immobilization amount is 5 times that of traditional particulate carriers, which can significantly improve the enzyme catalytic efficiency. Under the action of the immobilized enzyme, the substrate undergoes a biocatalytic reaction to produce hexanoic acid. In the reaction of ethyl hexanoate, the constant temperature water bath jacket 3 is connected to the circulating water pump and the constant temperature water tank through the water inlet on the bottom left and the water outlet on the top right, forming a circulating water system. This system can precisely control the reaction temperature and maintain a uniform and stable internal temperature of the reaction column body 1, providing a suitable environment for the enzyme catalytic reaction and facilitating the efficient reaction. The product generated by the reaction flows out from the product outlet of the reaction column body 1 and passes through the online refractometer 402 at the top inlet of the product collection tank 401. The online refractometer 402 monitors the concentration of ethyl hexanoate in real time and transmits the data to the central control console 403. The operator can adjust the reaction parameters in real time, such as the feed rate of the dual plunger feed pump 201, based on the data received by the central control console 403, to achieve precise control and automated operation of the reaction, ultimately completing an efficient and stable biocatalytic reaction and collecting the product in the product collection tank 401.

[0039] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0040] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An integrated hollow fiber membrane biocatalytic reactor, characterized in that, include: The reaction column body (1) is filled with enzyme carrier (101) inside. Substrate delivery unit (2) is located on the side of the reaction column body (1). The substrate delivery unit (2) includes a dual plunger feed pump (201), a substrate mixing tank (202), and a raw material storage tank (203). The dual plunger feed pump (201) is located on the lower left side of the reaction column body (1). The dual plunger feed pump (201) is connected to the substrate mixing tank (202). The raw material storage tank (203) is connected to the dual plunger feed pump (201) and to the reaction column body (1). A constant temperature water bath jacket (3) is wrapped around the outside of the reaction column body (1); Collection section (4) is provided on the side of the reaction column body (1) to collect the products after the reaction inside the reaction column body (1).

2. The integrated hollow fiber membrane biocatalytic reaction device according to claim 1, characterized in that: The enzyme carrier filler (101) is a hollow fiber membrane bundle. The enzyme carrier filler (101) is tightly filled inside the reaction column body (1). The enzyme carrier filler (101) is made of polyvinylidene fluoride, with an inner diameter of 0.5 mm, an outer diameter of 1.2 mm, and a length of 900 mm. The two ends of the hollow fiber membrane bundle are fixed to the inner wall of the reaction column body (1) with epoxy resin to form a sealed reaction cavity.

3. The integrated hollow fiber membrane biocatalytic reaction device according to claim 2, characterized in that: The specific surface area of ​​the hollow fiber membrane filled in the enzyme carrier (101) reaches 1000 m² / m³, and the enzyme immobilization capacity is 5 times that of traditional particulate carriers.

4. The integrated hollow fiber membrane biocatalytic reaction device according to claim 2, characterized in that: The reaction column body (1) has an enzyme solution injection port in the middle of the column body, and the enzyme solution is injected into the enzyme carrier filling (101) through the injection port.

5. The integrated hollow fiber membrane biocatalytic reaction device according to claim 1, characterized in that: There are two raw material storage tanks (203), which store hexanoic acid and ethanol respectively. Each of the two raw material storage tanks (203) is individually connected to a dual plunger feed pump (201), and the two dual plunger feed pumps (201) are connected to the same substrate mixing tank (202).

6. The integrated hollow fiber membrane biocatalytic reaction device according to claim 1, characterized in that: The constant temperature water bath jacket (3) has an inlet on the bottom left and an outlet on the top right. The constant temperature water bath jacket (3) is connected to the constant temperature water tank through a circulating water pump.

7. The integrated hollow fiber membrane biocatalytic reaction device according to claim 1, characterized in that: The collection unit (4) also has a product collection tank (401) located on the side of the reaction column body (1). An online refractometer (402) is installed at the top inlet of the product collection tank (401) and connected to the product outlet of the reaction column body (1). A central control panel (403) is installed on the side of the product collection tank (401). The online refractometer (402) monitors the concentration of ethyl hexanoate in real time and transmits the data to the central control panel (403).

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