A medium replenishing device in a cell incubator

Abstract

The utility model discloses a kind of medium replenishing devices in cell incubator, it is related to medium replenishing device technical field, the medium replenishing device includes cell incubator, two installation guides are provided on the inner wall of one side of cell incubator, the inside of two installation guides is all set to limit sliding slot, two inner slide blocks are movably arranged in the inside of limit sliding slot;It also includes: placing frame, it is welded and set on the outer wall of two inner slide blocks, and the inside recess of placing frame is arranged with multiple inner connecting rods, the spacing between adjacent two inner connecting rods is equal, the upper end of inner connecting rod is placed and set with culture medium, solve the existing culture medium injection device by setting the placement structure to facilitate quick replacement to take, but in the process of injection, added culture solution cannot be quantitatively handled, and injection needs to be carried out outside incubator, so that the environment changes after culture medium is taken out of incubator, cause cell culture failure.

Description

Technical Field

[0001] This utility model relates to the technical field of culture medium replenishment devices, specifically a culture medium replenishment device for a cell culture incubator. Background Technology

[0002] Cell culture incubators are laboratory equipment specifically designed for cell culture. By precisely controlling the temperature, humidity, and gas environment, they simulate the physiological conditions of tissues in vivo, supporting cell growth, proliferation, and maintenance of physiological state in vitro. Cell culture medium is discharged into the cell culture incubator and needs to be replenished during use.

[0003] For example, announcement number CN216513813U (named "A Culture Medium Injection Device") includes: a workbench; a placement structure disposed on the workbench, the placement structure including a drive motor and a rotating shaft, the drive motor being disposed inside the workbench, the bottom end of the rotating shaft being rotatably connected to the inside of the workbench, rotating gears being fixedly installed on both the output end of the drive motor and the outside of the rotating shaft, and the two rotating gears meshing externally, a rotating seat being fixedly installed on the top of the rotating shaft, a snap-fit ​​block being fixedly installed in the middle of the top of the rotating seat, a placement tray being snap-fitted on the top of the rotating seat, and a snap-fit ​​groove being formed in the middle of the bottom of the placement tray. The external snap-fit ​​block is snapped into the snap-fit ​​groove. The top of the placement tray has multiple evenly spaced slots. The placement structure is mainly used to place and fix test tubes. At the same time, multiple slots on the placement tray can hold multiple test tubes at the same time. It works with the rotating seat and can rotate with the rotating seat to achieve quick replacement of test tubes. After all test tubes have been filled, you only need to replace the next placement tray. This changes the traditional operation of placing and picking up test tubes one by one, thereby speeding up the filling speed. The rotating seat is automatically rotated by a drive motor. The operator only needs to control the filling operation head, making the filling operation easier and more convenient, thus improving work efficiency.

[0004] The aforementioned culture medium injection device facilitates quick replacement and retrieval through its placement structure. However, it cannot quantitatively process the added culture medium during the injection process and requires injection outside the incubator. This causes environmental changes after the culture medium is removed from the incubator, leading to cell culture failure. Therefore, we provide a culture medium replenishment device inside a cell culture incubator. Utility Model Content

[0005] The purpose of this invention is to provide a culture medium replenishment device in a cell culture incubator, in order to solve the problem mentioned in the background art that the existing culture medium injection device is easy to replace and take out through the placement structure, but cannot quantitatively process the added culture medium during the injection process, and needs to be injected outside the incubator, which causes the environment to change after the culture medium is taken out of the incubator, resulting in cell culture failure.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a culture medium replenishment device in a cell culture incubator, comprising a cell culture incubator, wherein two mounting guides are provided on one inner wall of the cell culture incubator, and each of the two mounting guides is provided with a limiting groove, and two inner sliding seats are movably arranged inside the limiting groove; Also includes: The placement frame is welded to the outer wall of the two inner sliding blocks, and multiple inner connecting rods are arranged in the inner groove of the placement frame. The spacing between two adjacent inner connecting rods is equal, and culture medium is placed at the upper end of the inner connecting rods. A transparent loading cylinder is positioned above the placement frame, and a first valve is connected to both outer walls of the transparent loading cylinder. A liquid outlet pipe is connected to the liquid outlet end of the first valve, and a metering tank is connected to the lower end of the liquid outlet pipe. The second valve is connected to the bottom center of the metering tank, and a replenishment pipe is connected to the outlet end of the second valve. A scale viewing window is integrally formed on the front end face of the metering tank.

[0007] Preferably, a limit block is provided at the front end of the mounting guide, and a connecting shaft is provided at the connection position between the limit block and the mounting guide. The limit block is rotatably connected to the mounting guide through the connecting shaft.

[0008] Preferably, two connecting sleeves are fixedly installed on the outer wall of the transparent loading cylinder by means of nail-free adhesive, and fastening bolts are provided at the connection positions between the two connecting sleeves and the inner wall of the cell culture box.

[0009] Preferably, the inner slide is provided with an auxiliary roller rotatably mounted inside via a rotating shaft, and the inner slide is rotatably connected to the limiting slide groove via the auxiliary roller.

[0010] Preferably, a gripping handle is provided on the front end face of the placement frame, and the gripping handle is welded to the placement frame.

[0011] Preferably, an injection port is integrally formed at the top center of the transparent loading cylinder, and the injection port is connected to the interior of the transparent loading cylinder.

[0012] Preferably, the replenishment pipes below the two metering tanks are located above the two placement frames, and the internal connecting rods inside the placement frames have a recessed structure.

[0013] Compared with the prior art, the beneficial effects of this utility model are: This invention moves the culture medium to the lower position of the replenishment tube, then opens the first valve to discharge the culture medium from the transparent loading cylinder through the outlet tube and inject it into the quantitative tank. The amount of culture medium in the quantitative tank is observed through the graduated viewing window. After quantifying the required amount of culture medium, the second valve is opened to discharge the measured amount of culture medium from the quantitative tank through the replenishment tube and inject it into the culture medium. The entire replenishment process is carried out in the cell culture incubator. This overcomes the problem that existing culture medium injection devices, while convenient for quick replacement and retrieval through their placement structure, cannot quantify the added culture medium during injection and require injection outside the incubator, causing environmental changes after the culture medium is removed from the incubator and resulting in cell culture failure. Attached Figure Description

[0014] Figure 1 This is a front view of the structure of the culture medium replenishment device in the cell culture incubator of this utility model. Figure 2 This is a bottom view of the structure of the culture medium replenishment device in the cell culture incubator of this utility model; Figure 3 This is a cross-sectional view of the internal structure of the culture medium replenishment device in the cell culture incubator of this utility model. Figure 4 This is an enlarged schematic diagram of part A of the present invention; Figure 5 This is an enlarged schematic diagram of part B of the present invention; In the diagram: 1. Cell culture incubator; 2. Mounting guide; 3. Inner slide; 4. Limiting block; 5. Connecting shaft; 6. Placement frame; 7. Inner connecting rod; 8. Grab handle; 9. Culture medium; 10. Transparent loading cylinder; 11. Connecting sleeve; 12. Quantitative container; 13. Fastening bolt; 14. Injection port; 15. Scale viewing window; 16. First valve; 17. Outlet pipe; 18. Second valve; 19. Replenishment pipe; 20. Limiting groove; 21. Auxiliary roller. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0016] Please see Figure 1-5An embodiment of this utility model is provided: a culture medium replenishment device in a cell culture box, including a cell culture box 1, two mounting guides 2 are provided on one inner wall of the cell culture box 1, and each of the two mounting guides 2 is provided with a limiting groove 20, and two inner sliding seats 3 are movably arranged inside the limiting groove 20. Also includes: The placement frame 6 is welded to the outer wall of the two inner sliding blocks 3, and multiple inner connecting rods 7 are arranged in the inner groove of the placement frame 6. The spacing between two adjacent inner connecting rods 7 is equal, and culture medium 9 is placed on the upper end of the inner connecting rod 7. A transparent loading cylinder 10 is positioned above the placement frame 6, and a first valve 16 is connected to both outer walls of the transparent loading cylinder 10. A liquid outlet pipe 17 is connected to the liquid outlet end of the first valve 16, and a metering tank 12 is connected to the lower end of the liquid outlet pipe 17. The second valve 18 is connected to the bottom center of the metering tank 12, and a replenishment pipe 19 is connected to the liquid outlet end of the second valve 18. A scale viewing window 15 is integrally formed on the front end face of the metering tank 12.

[0017] The cell culture incubator 1 is model: PH-mid / min. During use, the culture medium 9 is filled with the cells and culture medium to be cultured. The culture medium 9 is placed on the inner connecting rod 7 inside the placement frame 6. The placement frame 6 is slidably connected to the mounting guide 2 through the inner sliding seat 3. After the sliding installation is in place, the limiting block 4 is rotated to the vertical position to limit the inner sliding seat 3. When replenishment is required, the culture medium 9 is moved to the position below the replenishment tube 19. Then, the first valve 16 is opened first, and the culture medium inside the transparent loading cylinder 10 is discharged through the outlet tube 17 and injected into the quantitative tank 12. The amount of culture medium in the quantitative tank 12 is observed through the scale viewing window 15. After quantifying the required amount of culture medium, the second valve 18 is opened, and the quantitative amount of culture medium in the quantitative tank 12 is discharged through the replenishment tube 19 and injected into the culture medium 9. The entire replenishment process is carried out in the cell culture incubator.

[0018] Please see Figure 1 A limit stop 4 is provided at the front end of the mounting guide 2. A connecting shaft 5 is provided at the connection point between the limit stop 4 and the mounting guide 2. The limit stop 4 is rotatably connected to the mounting guide 2 through the connecting shaft 5. The limit stop 4 at the front end of the mounting guide 2 serves to facilitate the installation and limiting of the inner slide 3. Please refer to [link / reference]. Figure 2Two connecting sleeves 11 are fixedly installed on the outer wall of the transparent loading cylinder 10 using adhesive sleeves. Fastening bolts 13 are installed at the connection points between the two connecting sleeves 11 and the inner wall of the cell culture chamber 1. The two connecting sleeves 11, fixed on the outer wall of the transparent loading cylinder 10 using adhesive sleeves, serve to support and hold the transparent loading cylinder 10. Please refer to [link to relevant documentation]. Figure 5 An auxiliary roller 21 is rotatably mounted inside the inner slide block 3 via a rotating shaft. The inner slide block 3 is rolledly connected to the limiting slide groove 20 via the auxiliary roller 21. The auxiliary roller 21, which is rotatably mounted inside the inner slide block 3 via a rotating shaft, assists in the smooth movement of the inner slide block 3 and the limiting slide groove 20. Please refer to [link / reference]. Figure 1 A gripping handle 8 is provided on the front surface of the placement frame 6. The gripping handle 8 is welded to the placement frame 6. The gripping handle 8 on the front surface of the placement frame 6 facilitates the gripping and disassembly of the placement frame 6. Please refer to [link / reference]. Figure 2 A liquid injection port 14 is integrally formed at the center of the top of the transparent loading cylinder 10. The liquid injection port 14 is connected to the interior of the transparent loading cylinder 10. The integrally formed liquid injection port 14 at the center of the top of the transparent loading cylinder 10 facilitates the filling of culture medium into the transparent loading cylinder 10. Both the transparent loading cylinder 10 and the metering tank 12 are made of transparent plastic. Please refer to [link / reference]. Figure 1 and Figure 3 The replenishment pipes 19 below the two metering tanks 12 are located above the two placement frames 6 respectively, and the inner connecting rods 7 inside the placement frames 6 have a recessed structure.

[0019] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A culture medium replenishment device in a cell culture box, comprising a cell culture box (1), two mounting guides (2) are provided on one inner wall of the cell culture box (1), and a limiting groove (20) is provided inside the two mounting guides (2), and two inner sliding seats (3) are movably provided inside the limiting groove (20). Its features are: Also includes: The placement frame (6) is welded to the outer wall of the two inner sliding blocks (3), and multiple inner connecting rods (7) are arranged in the inner groove of the placement frame (6). The spacing between two adjacent inner connecting rods (7) is equal, and culture medium (9) is placed at the upper end of the inner connecting rod (7). A transparent loading cylinder (10) is positioned above the placement frame (6), and a first valve (16) is connected to both outer walls of the transparent loading cylinder (10). A liquid outlet pipe (17) is connected to the liquid outlet end of the first valve (16), and a metering tank (12) is connected to the lower end of the liquid outlet pipe (17). The second valve (18) is connected to the bottom center of the metering tank (12), and a replenishment pipe (19) is connected to the liquid outlet end of the second valve (18). A scale viewing window (15) is integrally formed on the front end surface of the metering tank (12).

2. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: A limit stop (4) is provided at the front end of the mounting guide (2), and a connecting shaft (5) is provided at the connection position between the limit stop (4) and the mounting guide (2). The limit stop (4) is rotatably connected to the mounting guide (2) through the connecting shaft (5).

3. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: Two connecting sleeves (11) are fixedly installed on the outer wall of the transparent loading cylinder (10) by nail-free adhesive. Fastening bolts (13) are provided at the connection positions between the two connecting sleeves (11) and the inner wall of the cell culture box (1).

4. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: The inner slide (3) is equipped with an auxiliary roller (21) that rotates through a rotating shaft. The inner slide (3) is connected to the limiting slide groove (20) by the auxiliary roller (21).

5. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: The front end of the placement frame (6) is provided with a gripping handle (8), which is welded to the placement frame (6).

6. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: The transparent loading cylinder (10) has an integrally formed liquid injection port (14) at the top center position, and the liquid injection port (14) is connected to the interior of the transparent loading cylinder (10).

7. The culture medium replenishment device in a cell culture incubator according to claim 1, characterized in that: The replenishment tubes (19) below the two quantitative tanks (12) are located above the two placement frames (6), and the inner connecting rods (7) inside the placement frames (6) are recessed structures.

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