Zebrafish embryo culture device

Abstract

The utility model discloses a zebra fish embryo culture device relates to zebra fish embryo culture technical field, including container and culture subassembly, and the container is equipped with the accommodation cavity, and the accommodation cavity is used for accommodating liquid, and culture subassembly is detachably arranged in the accommodation cavity, and culture subassembly includes the separating plate and the top cover detachably connected in the separating plate, and the separating plate is used for placing zebra fish embryo, and the separating plate is provided with a plurality of first openings, and the first opening is along the first direction and penetrates the separating plate, and the liquid in the accommodation cavity contacts zebra fish embryo, and the maximum inscribed circle diameter range of the projection of first opening on the plane perpendicular to the first direction is 0.7 0.9mm, and the side of top cover towards the separating plate is equipped with the groove, and the groove is used for accommodating zebra fish embryo. Zebra fish fry can be separated from culture subassembly through the first opening, so that zebra fish embryo and zebra fish fry can be separated automatically, and the problem that artificial separation zebra fish embryo and zebra fish fry is time -consuming and labor -intensive is solved.

Description

Technical Field

[0001] This utility model relates to the field of zebrafish embryo culture technology, and in particular to a zebrafish embryo culture device. Background Technology

[0002] Zebrafish, as a model organism, has been widely used in research and applications in medicine, pharmacy, and environmental toxicology. Zebrafish embryos typically develop rapidly in vitro, often using devices similar to cell culture plates. During rearing and breeding, it is necessary to promptly separate healthy juveniles that have ruptured their membranes from slow-developing or stopped-developing embryos. Changing the culture medium or water may also be necessary to ensure the normal growth of the juveniles. Current techniques require frequent use of pipettes to aspirate and separate unruptured embryos one by one, or to change the culture medium or water, resulting in low efficiency and being time-consuming and labor-intensive.

[0003] Therefore, how to provide a zebrafish embryo culture device to solve the problem of time-consuming and labor-intensive artificial separation of zebrafish embryos and zebrafish larvae is a technical problem that needs to be solved by those skilled in the art. Utility Model Content

[0004] The purpose of this invention is to provide a zebrafish embryo culture device to solve the problem of time-consuming and labor-intensive manual separation of zebrafish embryos and juvenile zebrafish.

[0005] To achieve the above objectives, this utility model provides a zebrafish embryo culture device, including a container and a culture component. The container has a receiving cavity for containing liquid. The culture component is detachably disposed within the receiving cavity. The culture component includes a separation plate and a top cover detachably connected to the separation plate. The separation plate is used to place zebrafish embryos. The separation plate has several first openings that penetrate the separation plate along a first direction so that the liquid in the receiving cavity contacts the zebrafish embryos. The maximum inscribed circle diameter of the projection of the first opening onto a plane perpendicular to the first direction ranges from 0.7 to 0.9 mm. A groove is provided on the side of the top cover facing the separation plate for containing zebrafish embryos.

[0006] In one possible implementation, the separation plate is further provided with a plurality of filter columns extending along a first direction. The filter columns are located in the tank, and the distance between two adjacent filter columns in a second direction is less than 0.9 mm and / or the distance in a third direction is less than 0.9 mm. The second direction is perpendicular to the third direction, and both the second direction and the third direction are perpendicular to the first direction.

[0007] In one possible implementation, at least one fixing position is formed between a plurality of filter columns adjacent to the first opening. One fixing position is used to place a zebrafish embryo, and the filter column is used to prevent the zebrafish embryo from detaching from its corresponding fixing position.

[0008] In one possible implementation, the separation plate is connected to a first support member, and the container is provided with a second support member located within the receiving cavity and used to support the first support member so that the separation plate is fixed relative to the container.

[0009] In one possible implementation, the separation plate is connected to a rod, and the inner wall of the container is provided with a protrusion with a socket adapted to the rod. The rod is movably inserted into the socket so that the separation plate is mounted in the receiving cavity.

[0010] In one possible implementation, a backflow prevention guide shroud is also included. The backflow prevention guide shroud is provided with a flow channel, which includes a first end with a larger opening and a second end with a smaller opening. The first end is detachably connected to the bottom of the separation plate, and the flow channel communicates with all the first openings.

[0011] In one possible implementation, the peripheral side of the separation plate is provided with a snap-fit ​​groove, and the top cover is provided with a snap-fit ​​connector adapted to the snap-fit ​​groove. The snap-fit ​​connector is detachably snapped into the snap-fit ​​groove to fix the separation plate and the top cover relative to each other.

[0012] In one possible implementation, the bottom of the tank is provided with an arc-shaped groove, and the arc-shaped groove corresponds one-to-one with the fixing position.

[0013] In one possible implementation, the length of the filter column in the first direction ranges from 9 to 11 mm.

[0014] In one possible implementation, the container is further provided with an inlet and an outlet. The inlet is connected to the receiving cavity for liquid to flow into the receiving cavity, and the outlet is connected to the receiving cavity for liquid to flow out of the receiving cavity.

[0015] Compared with the prior art, the technical solution provided by this utility model has at least the following beneficial effects: After the separation plate and cover plate are assembled, they are placed in the container cavity. The liquid in the container cavity enters the tank through the first opening on the separation plate and contacts the zebrafish embryo. The maximum inscribed circle diameter of the projection of the first opening on the plane perpendicular to the first direction is 0.7-0.9 mm, which is smaller than the size of the zebrafish embryo, so as to prevent the zebrafish embryo from detaching from the culture component. When the zebrafish embryo is cultured into zebrafish larvae, the zebrafish larvae can detach from the culture component through the first opening, so that the zebrafish embryo and zebrafish larvae can separate on their own, saving the trouble of manual separation and solving the problem of time-consuming and laborious manual separation of zebrafish embryos and zebrafish larvae. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the zebrafish embryo culture device provided in an embodiment of the present invention;

[0018] Figure 2 This is a schematic diagram of the structure of the culture component provided in an embodiment of the present invention;

[0019] Figure 3 This is a schematic diagram of the structure when the first opening is a circular hole, as provided in an embodiment of the present utility model;

[0020] Figure 4 This is a schematic diagram of the structure when the first opening is a square hole, as provided in an embodiment of the present utility model;

[0021] Figure 5 This is a schematic diagram of the separation plate provided in an embodiment of the present utility model.

[0022] in:

[0023] 100 - Container, 110 - Receiving cavity, 120 - Second support member;

[0024] 200-Cultivation component, 210-Separation plate, 211-First opening, 212-Filter column, 213-Snap-fit ​​groove, 220-Top cover, 221-Tank body, 222-Snap-fit ​​connector, 223-Arc-shaped groove, 230-First support component;

[0025] 300-Anti-backflow guide shield. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] To enable those skilled in the art to better understand the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0028] In the description of this utility model, it should be understood that the terms "upper", "lower", "left" and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the position or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations of this utility model.

[0029] The purpose of this invention is to provide a zebrafish embryo culture device to solve the problem of time-consuming and labor-intensive manual separation of zebrafish embryos and juvenile zebrafish.

[0030] It should be noted that in this embodiment, the Z direction in the attached figure is defined as the first direction, the X direction as the second direction, and the Y direction as the third direction. The second direction is perpendicular to the third direction, and both the second direction and the third direction are perpendicular to the first direction.

[0031] Please see Figures 1 to 5 To achieve the above objectives, this utility model provides a zebrafish embryo culture device, including a container 100 and a culture component 200. The container 100 is provided with a receiving cavity 110. The shape of the container 100 and the receiving cavity 110 can be, but is not limited to, a cuboid, a cylinder, etc., and the volume of the receiving cavity 110 can be set according to actual needs. The receiving cavity 110 is used to contain liquid, which can be, but is not limited to, culture medium or water. The culture component 200 is detachably disposed within the receiving cavity 110. The culture component 200 includes a separation plate 210 and a top cover 220 detachably connected to the separation plate 210. The separation plate 210 is used to place zebrafish embryos. The separation plate 210 has a plurality of first openings 211, which penetrate the separation plate 210 along a first direction, so that the liquid in the receiving cavity 110 contacts the zebrafish embryos, providing culture conditions for the zebrafish embryos. The maximum inscribed circle diameter of the projection of the first opening 211 onto a plane perpendicular to the first direction ranges from 0.7 to 0.9 mm. The top cover 220 has a trough 221 on the side facing the separation plate 210, which is used to hold zebrafish embryos.

[0032] It should be noted that zebrafish embryos are elastic spherical structures, typically 1-1.5 mm in diameter. After hatching, the widest part of the zebrafish larvae's body is approximately 0.6 mm, referring to the maximum dimension perpendicular to the line connecting the head and tail. The maximum inscribed circle diameter of the projection of the first opening 211 onto a plane perpendicular to the first direction ranges from 0.7-0.9 mm, thus providing both obstruction for the zebrafish embryo and passage for the larvae. When the culture medium or water needs to be changed, the culture component 200 can be directly removed from the culture medium or water and replaced with fresh culture medium or water.

[0033] After assembly, the separation plate 210 and the cover plate are placed in the receiving cavity 110 of the container 100. The liquid in the receiving cavity 110 enters the tank 221 through the first opening 211 on the separation plate 210 and comes into contact with the zebrafish embryos. The maximum inscribed circle diameter of the projection of the first opening 211 onto a plane perpendicular to the first direction is in the range of 0.7-0.9 mm, which is smaller than the size of the zebrafish embryos, thus preventing the zebrafish embryos from detaching from the culture component 200. When the zebrafish embryos are cultured into zebrafish larvae, the zebrafish larvae can detach from the culture component 200 through the first opening 211, allowing the zebrafish embryos and zebrafish larvae to separate on their own, eliminating the trouble of manual separation and solving the problem of time-consuming and labor-intensive manual separation of zebrafish embryos and zebrafish larvae.

[0034] In one possible implementation, the separation plate 210 is further provided with a plurality of filter columns 212 extending along a first direction. The filter columns 212 are located within the tank 221, and the distance between two adjacent filter columns 212 is less than 0.9 mm in a second direction and / or less than 0.9 mm in a third direction. That is, it includes three cases: the distance between two adjacent filter columns 212 is less than 0.9 mm in the second direction, the distance between two adjacent filter columns 212 in a third direction is less than 0.9 mm, and the distance between two adjacent filter columns 212 is less than 0.9 mm in both the second and third directions. Limiting the distance between two adjacent filter columns 212 restricts the movement of zebrafish embryos within the tank 221, making the position of the zebrafish embryos relatively fixed. This facilitates counting the number of zebrafish embryos hatched to calculate the hatching rate and avoids unnecessary collisions between adjacent zebrafish embryos. The distance between two adjacent filter columns 212 is greater than 0.7 mm in the second direction and / or greater than 0.7 mm in the third direction, so as to avoid the distance between two adjacent filter columns 212 being too narrow, which would excessively compress the cultivation space for zebrafish embryos.

[0035] In one possible implementation, at least one fixing position is formed among a plurality of filter columns 212 adjacent to the first opening 211. One fixing position is used to place a zebrafish embryo, and the filter column 212 is used to prevent the zebrafish embryo from detaching from its corresponding fixing position. The first opening 211 can be, but is not limited to, circular, elliptical, rectangular, triangular, etc. When the first opening 211 is circular, at least three filter columns 212 can be evenly arranged around the circle. For example, three filter columns 212 are located near the trisection points of the first opening 211, with an included angle of 120° between them; four filter columns 212 are located near the trisection points of the first opening 211, with an included angle of 90° between them. When the first opening 211 is rectangular and the longer side of the rectangle is relatively long, two sets of filter columns 212 can be arranged on both sides of the first opening 211 in the second direction. Each set of filter columns 212 includes multiple filter columns 212 that are evenly and spaced apart along the third direction, so that the two sets of filter columns 212 and a first opening 211 form multiple fixed positions arranged sequentially along the third direction. For example, when the two sets of filter columns 212 are symmetrically arranged, a fixed position is formed between four adjacent filter columns 212 arranged in a square layout; when the two sets of filter columns 212 are staggered, a fixed position is formed between three adjacent filter columns 212 arranged in a triangular layout.

[0036] In one possible implementation, the diameter of the filter column 212 can be set to 0.6-0.8 mm, and the length of the filter column 212 in the first direction is in the range of 9-11 mm, providing sufficient space for the zebrafish embryos in the first direction. The bottom of the tank 221 has an arc-shaped groove 223, which corresponds one-to-one with the fixing position, further expanding the living space of the zebrafish embryos in the fixing position to avoid squeezing them. The arc-shaped groove 223 is preferably a semi-circular groove, and its size is adapted to the fixing position. The top cover 220 can be made of a transparent material, which can be, but is not limited to, transparent polymer materials such as acrylic, to facilitate observation of the zebrafish embryo culture. The top cover 220 can also have through holes to facilitate the flow of culture medium or water.

[0037] In one possible implementation, the separating plate 210 is connected to a first support member 230, which is disposed on the left and right end faces of the separating plate 210 in a second direction, and the first support member 230 is located at the edge of the left end face in a third direction. The container 100 is provided with a second support member 120, which is located within the receiving cavity 110. The second support member 120 corresponds one-to-one with the first support member 230, and the upper surface of the second support member 120 is used to contact the lower surface of the first support member 230 and to support the first support member 230, so that the separating plate 210 is fixed relative to the container 100. In addition, a positioning component can be added. The positioning component may include a positioning groove and a positioning post. One of the positioning groove and the positioning post is located at the bottom of the first support member 230 and the other is located at the top of the second support member 120. This is used to ensure that the positions of the first support member 230 and the second support member 120 are accurate during the process of the first support member 230 and the second support member 120 moving towards each other, and at the same time, to prevent the first support member 230 from sliding relative to the second support member 120 after it has moved into place.

[0038] In one possible implementation, the separating plate 210 is connected to a rod, and the inner wall of the container 100 is provided with a protrusion. The protrusion has a socket adapted to the rod, and the rod can be movably inserted into the socket so that the separating plate 210 is mounted in the receiving cavity 110. Specifically, the separating plate 210 is provided with a blind hole for the rod to be inserted. Moving the rod can adjust the depth of the rod inserted into the blind hole. First, most of the rod is retracted into the blind hole, and then the end of the rod outside the blind hole is moved and inserted into the socket. By adjusting the ratio of the rod in the socket and the blind hole, the separating plate 210 can be mounted in the receiving cavity 110. The length of the rod and the blind hole in the second direction can be set to be relatively long to ensure the length of the rod inserted into the blind hole and the socket, thereby ensuring the stability of the separating plate 210.

[0039] In one possible implementation, the zebrafish embryo culture device further includes an anti-backflow guide hood 300. The anti-backflow guide hood 300 has a guide channel, which includes a first end with a larger opening and a second end with a smaller opening. The first end is detachably connected to the bottom of the separation plate 210. This connection method can be, but is not limited to, a snap-fit ​​connection or a bolt connection, facilitating the disassembly, cleaning, and disinfection of the anti-backflow guide hood 300. The guide channel communicates with all the first openings 211, allowing zebrafish larvae swimming out of the first openings 211 to swim into the receiving cavity 110. The anti-backflow guide hood 300 is generally in the shape of an inverted cone structure, which can be, but is not limited to, a cone or a pyramid. The smaller opening at the second end of the guide channel effectively reduces the probability of zebrafish larvae returning to the guide channel within the container 100, thereby preventing the zebrafish larvae from further reversing through the first opening 211 of the separation plate 210 and thus avoiding affecting the culture of unhatched zebrafish embryos.

[0040] In one possible implementation, the top cover 220 corresponds in shape to the separation plate 210. This reduces the impact of the external environment and provides some fixation for the zebrafish embryo in the fixed position. The separation plate 210 has a snap-fit ​​groove 213 on its peripheral side, and the top cover 220 has a snap-fit ​​connector 222 adapted to the snap-fit ​​groove 213. The snap-fit ​​connector 222 is detachably snapped into the snap-fit ​​groove 213 to fix the separation plate 210 and the top cover 220 relative to each other. This snap-fit ​​process involves the top cover 220 being directly fastened onto the separation plate 210. Alternatively, an existing detachable connection structure can be used, such as, but is not limited to, bolt connections or slide rail connections.

[0041] In one possible implementation, the container 100 is further provided with an inlet and an outlet. The inlet is connected to the receiving cavity 110 for fresh liquid to flow into the receiving cavity 110, and the outlet is connected to the receiving cavity 110 for liquid to flow out of the receiving cavity 110. The inlet and outlet facilitate the replacement and circulation of the liquid within the container 100. For example, a pump can be provided, and the inlet, outlet, and pump can be connected through a pipeline to achieve automatic replacement of the culture medium or water within the container 100. It should be noted that both the inlet and outlet are equipped with filters to prevent zebrafish fry from being drawn into the pipeline.

[0042] It should be noted that in this specification, relational terms such as first and second are used only to distinguish one entity from several other entities, and do not necessarily require or imply any such actual relationship or order between these entities.

[0043] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0044] This article uses specific examples to illustrate the principles and implementation methods of this utility model. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of this utility model.

Claims

1. A zebrafish embryo culture device, characterized in that, The device includes a container (100) and a culture assembly (200). The container (100) has a receiving cavity (110) for containing liquid. The culture assembly (200) is detachably disposed within the receiving cavity (110). The culture assembly (200) includes a separation plate (210) and a top cover (220) detachably connected to the separation plate (210). The separation plate (210) is used to hold zebrafish embryos. The separation plate (210) has several openings. The first opening (211) extends through the separation plate (210) along a first direction to allow the liquid in the receiving cavity (110) to contact the zebrafish embryo. The maximum inscribed circle diameter of the projection of the first opening (211) onto a plane perpendicular to the first direction is in the range of 0.7-0.9 mm. The top cover (220) has a groove (221) on the side facing the separation plate (210) for accommodating the zebrafish embryo.

2. The zebrafish embryo culture device according to claim 1, characterized in that, The separation plate (210) is also provided with a plurality of filter columns (212) extending along the first direction. The filter columns (212) are located in the tank (221). The distance between two adjacent filter columns (212) in the second direction is less than 0.9 mm and / or the distance in the third direction is less than 0.9 mm. The second direction is perpendicular to the third direction, and both the second direction and the third direction are perpendicular to the first direction.

3. The zebrafish embryo culture device according to claim 2, characterized in that, At least one fixing position is formed between a plurality of filter columns (212) adjacent to the first opening (211), one of the fixing positions is used to place one of the zebrafish embryos, and the filter column (212) is used to restrict the zebrafish embryo from detaching from its corresponding fixing position.

4. The zebrafish embryo culture apparatus according to any one of claims 1 to 3, characterized in that, The separation plate (210) is connected to a first support member (230), and the container (100) is provided with a second support member (120). The second support member (120) is located in the receiving cavity (110) and is used to support the first support member (230) so that the separation plate (210) is fixed relative to the container (100).

5. The zebrafish embryo culture apparatus according to any one of claims 1 to 3, characterized in that, The separation plate (210) is connected to a plug rod, and the inner wall of the container (100) is provided with a protrusion. The protrusion is provided with a plug hole that is adapted to the plug rod. The plug rod is movably inserted into the plug hole so that the separation plate (210) is mounted in the receiving cavity (110).

6. The zebrafish embryo culture apparatus according to any one of claims 1 to 3, characterized in that, It also includes a backflow prevention guide hood (300), which is provided with a flow channel. The flow channel includes a first end with a larger opening and a second end with a smaller opening. The first end is detachably connected to the bottom of the separation plate (210), and the flow channel is connected to all the first openings (211).

7. The zebrafish embryo culture apparatus according to any one of claims 1 to 3, characterized in that, The peripheral side of the separation plate (210) is provided with a snap-fit ​​groove (213), and the top cover (220) is provided with a snap-fit ​​connector (222) adapted to the snap-fit ​​groove (213). The snap-fit ​​connector (222) is detachably snapped into the snap-fit ​​groove (213) to fix the separation plate (210) and the top cover (220) relative to each other.

8. The zebrafish embryo culture device according to claim 3, characterized in that, The bottom of the groove (221) is provided with an arc-shaped groove (223), and the arc-shaped groove (223) corresponds one-to-one with the fixed position.

9. The zebrafish embryo culture device according to claim 3, characterized in that, The length of the filter column (212) in the first direction ranges from 9 to 11 mm.

10. The zebrafish embryo culture apparatus according to any one of claims 1 to 3, characterized in that, The container (100) is also provided with an inlet and an outlet. The inlet is connected to the receiving cavity (110) for liquid to flow into the receiving cavity (110), and the outlet is connected to the receiving cavity (110) for liquid to flow out of the receiving cavity (110).

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