A phage shaking culture device

Abstract

The utility model discloses a kind of phage oscillation culture devices, it is related to microbiological culture equipment technical field.The device includes box, motor, runner, tray, limiting component, oscillation component and elastic support component.Motor is fixed in box, and motor output shaft end is connected with runner with skirt and convex buckling;Tray is suspended in the upper of runner, and is equipped with through hole and placing groove;Limiting component is coaxially fixed in runner, and tray is limited;Three oscillation components are distributed in the periphery of runner with equal angle, including double roller in contact with skirt;Three elastic support components support tray corresponding to oscillation component.The device is driven runner rotation by motor, utilizes convex buckling and double roller cooperation, and realizes tray alternate inclination and horizontal, achieves efficient oscillation by combining elastic support component.Compared with prior art, the utility model oscillation mode is more suitable for phage culture demand, and mixed uniformity is stronger, stability is higher, and structure layout is reasonable, operation and maintenance are convenient, and application scope is wider.

Description

Technical Field

[0001] This utility model belongs to the technical field of microbial culture equipment, specifically relating to a bacteriophage shaking culture device, which is particularly suitable for efficient shaking culture of bacteriophages. Background Technology

[0002] In the field of microbial culture, shaking culture devices are one of the key pieces of equipment. They achieve functions such as nutrient mixing and gas exchange by driving the culture container to vibrate, ensuring the healthy growth of microorganisms. Utility model patent CN206454873U discloses a joint-suspension rotary shaking device for a culture shaker. This device achieves rotary oscillation of the shaker through the cooperation of lower fixed seats, lower joint bearings, a swing rod, upper joint bearings, and upper fixed seats at both ends of the shaker. While this device has advantages such as simple structure and small footprint, it still has certain limitations in practical applications: The oscillation mechanism relies on a pendulum driving a joint bearing to swing left and right and rotate back and forth, resulting in a relatively simple oscillation trajectory. For microbial cultures like bacteriophages, which require high mixing uniformity, achieving the desired oscillation effect is difficult. Furthermore, since oscillation is achieved solely through the connection between the pendulum and the joint bearing, stability needs improvement during prolonged high-frequency oscillation. Given the shortcomings of existing technology, there is an urgent need for an oscillation culture device with a superior oscillation mode, better mixing effect, higher stability, and suitability for bacteriophage culture. Utility Model Content

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a phage oscillation culture device that solves the problems of single oscillation trajectory, insufficient mixing uniformity, and need to improve stability of existing culture oscillators.

[0004] This invention is implemented as follows: a phage oscillation culture device, comprising: The housing contains a motor. A rotating wheel is fixed to the output shaft end of a motor. The outer side of the rotating wheel is provided with a skirt, and the skirt is provided with an upward protruding protrusion. The tray is suspended above the rotating wheel; A limiting component is located between the rotating wheel and the tray, and is coaxially fixed on the rotating wheel. The limiting component limits the tray to the rotating wheel. Three oscillating components are distributed at equal angles around the outer periphery of the rotating wheel. Each oscillating component is equipped with a first roller and a second roller, which respectively contact the upper and lower surfaces of the skirt. Three elastic support components are correspondingly installed on the three oscillation components to provide elastic support for the pallet.

[0005] Preferably, the tray has a through hole in its center and a placement groove distributed at equal angles around the through hole on its top surface.

[0006] Preferably, the middle part of the through hole is a spherical cavity, and the upper and lower parts are trumpet-shaped cavities.

[0007] Preferably, the limiting component includes a chassis, which is fixed to the top surface of the rotating wheel by bolts. A threaded rod is threadedly connected to the center of the chassis, and a limiting ball is fixed at the top of the threaded rod within the spherical cavity of the through hole.

[0008] Preferably, the oscillation component includes a support fixed inside the housing, and a sector-shaped disk is hinged to the top of the support via a pivot. The first roller and the second roller are rotatably connected to the side of the sector-shaped disk facing the rotating wheel.

[0009] Preferably, the elastic support component includes an oscillating spring, with a first spring pad fixed to the upper end of the oscillating spring and the first spring pad fixed to the bottom surface of the tray, and a second spring pad fixed to the lower end of the oscillating spring and the second spring pad fixed to the sector-shaped disk.

[0010] Preferably, when the first roller and the second roller on one of the oscillating components are in the raised position, the fan-shaped disk on the oscillating component lifts the tray by the oscillating spring, so that the tray is in an inclined state.

[0011] Preferably, when the first roller and the second roller on the three oscillating components are simultaneously in the non-convex position, the fan-shaped disks on the three oscillating components keep the tray in a horizontal state through the oscillation spring.

[0012] Compared with related technologies, the phage shaking culture device provided by this utility model has the following beneficial effects: 1. This utility model adopts an alternating tilting and horizontal oscillation mode, which, compared with a single gyratory oscillation, can more fully stir the culture system, allowing the bacteriophage to fully contact with nutrients and gases, thereby improving the culture effect; 2. The three oscillation components of this utility model are distributed at equal angles, and together with the elastic support component and the limiting component, they ensure that the force is balanced and the displacement is controllable during the oscillation of the tray, avoid shaking or displacement under high-frequency oscillation, and reduce noise at the same time. 3. The oscillation intensity of this invention can be adjusted by adjusting parameters such as motor speed and convex height, which can meet the cultivation needs of bacteriophages and other microorganisms; 4. The modular design of each component of this utility model is compact and logically clear, making installation and disassembly convenient and reducing subsequent maintenance costs; the suspended design of the tray reduces direct friction with other components and extends service life. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of a phage oscillation culture device proposed in this utility model; Figure 2 This is a front view schematic diagram of a phage oscillation culture device proposed in this utility model; Figure 3 This is a schematic diagram of the tray and its components proposed in this utility model; Figure 4 This is a schematic diagram of the cross-sectional structure of the tray and its components proposed in this utility model; Figure 5 This is a schematic diagram of the rotary wheel structure proposed in this utility model; Figure 6 This is a schematic diagram of the tray half-section structure proposed in this utility model; Figure 7 This is a schematic diagram of the limiting component structure proposed in this utility model; Figure 8 This is a schematic diagram of the oscillation component and elastic support component proposed in this utility model.

[0014] In the diagram: 1. Housing; 2. Motor; 3. Rotary wheel; 31. Skirt; 32. Protrusion; 4. Tray; 41. Through hole; 42. Placement slot; 5. Limiting component; 51. Chassis; 52. Threaded rod; 53. Limiting ball; 6. Vibration component; 61. Support; 62. Sector-shaped disk; 63. Rotating shaft; 64. First roller; 65. Second roller; 7. Elastic support component; 71. Vibration spring; 72. First spring pad; 73. Second spring pad. Detailed Implementation

[0015] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings of this application are used to distinguish different objects, not to describe a particular order.

[0016] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0017] Please see Figure 1 and Figure 2 A phage oscillation culture device, the specific structure of which is as follows: 1. Housing 1: The motor 2 is fixed inside, providing power support for the operation of the entire device and serving as the installation base for all components.

[0018] 2. Rotary wheel 3: Fixed to the output shaft end of motor 2, rotating synchronously with the motor output shaft. The outer side of the rotary wheel 3 is provided with a skirt 31, and the skirt 31 is provided with an upward protruding protrusion 32, which provides a trigger structure for realizing the tilting oscillation of the tray 4.

[0019] 3. Tray 4: Suspended above the rotor 3, used to hold phage culture containers. The tray 4 has a through hole 41 in the center, with a spherical cavity in the middle and horn-shaped cavities at the top and bottom, which facilitates positioning and flexible swinging in conjunction with the limiting component 5; the top surface of the tray 4 has placement grooves 42 distributed at equal angles around the through hole 41, which can accurately position the culture containers and prevent the containers from shifting during shaking.

[0020] 4. Limiting component 5: Located between the rotating wheel 3 and the tray 4, and coaxially fixed to the rotating wheel 3, it is used to limit the tray 4 to the rotating wheel 3 without affecting the oscillation of the tray 4. It includes a chassis 51, which is fixed to the top surface of the rotating wheel 3 by bolts. A threaded rod 52 is threadedly connected to the center of the chassis 51. The top end of the threaded rod 52 is fixed with a limiting ball 53 that is limited to the spherical cavity of the through hole 41. The structure of the cooperation between the spherical cavity and the limiting ball 53 allows the tray 4 to tilt and swing flexibly within a certain range, while limiting the axial displacement of the tray 4.

[0021] 5. Oscillating Components 6: There are three in total, distributed at equal angles around the outer periphery of the rotating wheel 3 to ensure balanced force on the tray 4. The oscillating components 6 include a support 61, which is fixed inside the housing 1. The top of the support 61 is hinged to a fan-shaped disk 62 via a rotating shaft 63. The fan-shaped disk 62 is rotatably connected to a first roller 64 and a second roller 65 facing the rotating wheel 3. The first roller 64 and the second roller 65 respectively contact the upper and lower surfaces of the skirt 31. The oscillation of the fan-shaped disk 62 is achieved through the rolling contact between the rollers and the skirt 31 and the protrusion 32.

[0022] 6. Elastic support components 7: Three in total, correspondingly mounted on the three oscillating components 6, for elastic support of the tray 4. Each component includes an oscillating spring 71, with a first spring pad 72 fixed to the upper end of the spring 71 and fixed to the bottom surface of the tray 4. A second spring pad 73 is fixed to the lower end of the spring 71 and fixed to the sector-shaped disk 62. The elastic extension and contraction of the oscillating spring 71, combined with the oscillation of the sector-shaped disk 62, causes the tray 4 to oscillate. When the first roller 64 and second roller 65 on one of the oscillating components 6 are in the protruding position 32, the sector-shaped disk 62 on that oscillating component 6 lifts the tray 4 via the oscillating spring 71, causing the tray 4 to tilt. When the first roller 64 and second roller 65 on all three oscillating components 6 are simultaneously in the non-protruding position 32, the sector-shaped disks 62 on all three oscillating components 6 keep the tray 4 in a horizontal position via the oscillating spring 71. Motor 2 drives the rotating wheel 3 to rotate continuously, and the protrusion 32 contacts the rollers of the three oscillating components 6 in sequence, so that the tray 4 alternates between tilted and horizontal states to achieve efficient oscillation.

[0023] The working principle of this utility model is as follows: 1. First, place the phage culture container in the placement slot 42 of the tray 4. The structure of the placement slot 42 can accurately position the culture container and prevent it from shifting during shaking.

[0024] 2. Start motor 2. The output shaft of motor 2 drives the rotating wheel 3 to rotate at a constant speed. The skirt 31 and protrusion 32 on the outer side of the rotating wheel 3 rotate synchronously with the rotating wheel 3.

[0025] 3. When the protrusion 32 on the rotating wheel 3 rotates to contact the first roller 64 and the second roller 65 of a certain oscillating component 6, the protrusion 32 pushes the fan-shaped disk 62 to swing upward around the rotating shaft 63. The fan-shaped disk 62 drives the corresponding side of the tray 4 to lift upward through the oscillation spring 71, so that the tray 4 is in an inclined state.

[0026] 4. As the rotating wheel 3 continues to rotate, the protrusion 32 disengages from the roller of the oscillating component 6, and the fan-shaped disk 62 of the oscillating component 6 returns to its original position under the elastic reset action of the oscillating spring 71, and the tray 4 falls to the corresponding side; at the same time, the protrusion 32 contacts the roller of the next oscillating component 6 in turn, repeating the above lifting action.

[0027] 5. When none of the rollers of the three oscillating components 6 are in contact with the protrusion 32, the three oscillating springs 71 are in a balanced state and the tray 4 remains horizontal.

[0028] 6. Motor 2 runs continuously, and tray 4 cycles between tilted and horizontal states to achieve efficient oscillation of the phage culture system; after the culture is completed, turn off motor 2 and remove the culture container.

[0029] This invention can adapt to different phage culture scenarios by adjusting the oscillation frequency of the motor 2 and changing the tilt angle of the tray 4 by replacing the protrusions 32 with different heights, according to actual culture needs. The above description is merely a preferred embodiment of this invention and is not intended to limit the invention. Various modifications and variations can be made to this invention by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this invention should be included within the protection scope of this invention.

Claims

1. A phage shaking culture device, characterized in that, include: The housing (1) has a motor (2) fixed inside it; The rotating wheel (3) is fixed to the output shaft end of the motor (2). The outer side of the rotating wheel (3) is provided with a skirt (31), and the skirt (31) is provided with an upward protruding protrusion (32). The tray (4) is suspended above the turntable (3); The limiting component (5) is located between the rotating wheel (3) and the tray (4) and is coaxially fixed on the rotating wheel (3). The limiting component (5) limits the tray (4) to the rotating wheel (3). Three oscillating components (6) are distributed at equal angles around the rotating wheel (3). Each oscillating component (6) is provided with a first roller (64) and a second roller (65). The first roller (64) and the second roller (65) respectively contact the upper and lower surfaces of the skirt (31). Three elastic support components (7) are respectively set on three oscillating components (6) for elastic support of the tray (4).

2. The phage shaking culture device as described in claim 1, characterized in that, The tray (4) has a through hole (41) in the center and a placement groove (42) with equal angles around the through hole (41) on the top surface of the tray (4).

3. The phage shaking culture device as described in claim 2, characterized in that, The middle part of the through hole (41) is a spherical cavity, and the upper and lower parts are trumpet-shaped cavities.

4. The phage shaking culture device as described in claim 3, characterized in that, The limiting component (5) includes a chassis (51), which is fixed to the top surface of the rotating wheel (3) by bolts. A threaded rod (52) is threadedly connected to the center of the chassis (51), and a limiting ball (53) is fixed at the top of the threaded rod (52) within the spherical cavity of the through hole (41).

5. The phage shaking culture device as described in claim 1, characterized in that, The oscillation component (6) includes a support (61), which is fixed inside the housing (1). A fan-shaped disk (62) is hinged to the top of the support (61) via a rotating shaft (63). The first roller (64) and the second roller (65) are rotatably connected to the side of the fan-shaped disk (62) facing the rotating wheel (3).

6. The phage shaking culture device as described in claim 5, characterized in that, The elastic support component (7) includes an oscillating spring (71), the upper end of which is fixed with a first spring pad (72), the first spring pad (72) being fixed to the bottom surface of the tray (4), and the lower end of which is fixed with a second spring pad (73), the second spring pad (73) being fixed to the fan-shaped disk (62).

7. The phage shaking culture device as described in claim 6, characterized in that, When the first roller (64) and the second roller (65) on one of the oscillating components (6) are in the protruding (32) position, the fan-shaped disk (62) on the oscillating component (6) lifts the tray (4) through the oscillating spring (71), so that the tray (4) is in an inclined state.

8. The phage shaking culture device as described in claim 7, characterized in that, When the first roller (64) and the second roller (65) on the three oscillating components (6) are simultaneously in the non-convex (32) position, the fan-shaped disk (62) on the three oscillating components (6) keeps the tray (4) in a horizontal state through the oscillation spring (71).

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