An organoid culture device

Abstract

The utility model relates to the technical field of organoid cell culture, specifically a kind of organoid culture device, including liquid storage box and culture box, the liquid storage box and culture box are all provided with liquid inlet and liquid outlet;It further includes support, the support includes the support platform of being arranged in several up and down, peristaltic pump is arranged on the support;The support platform on top places the liquid storage box, the support platform on below places the culture box, the liquid outlet of the liquid storage box is connected in proper order with the liquid inlet of several culture boxes from top to bottom in succession by hose, the liquid outlet of several culture boxes is connected in proper order from top to bottom in succession, the liquid outlet of bottom culture box is connected with the liquid inlet of liquid storage box by liquid return hose, and liquid return hose passes through the peristaltic pump.The utility model low cost can realize batch organoid culture.

Description

Technical Field

[0001] This utility model relates to the field of organoid cell culture technology, specifically an organoid culture device. Background Technology

[0002] Organoids are three-dimensional cell cultures possessing the structural and functional characteristics of organs, formed through in vitro culture of adult stem cells, pluripotent stem cells, or tissue progenitor cells. Organoid culture induces cell self-organization and differentiation through the extracellular matrix and specific growth factors, forming spatial structures containing various organ-specific cell types. Current culture methods include static and dynamic culture. Dynamic culture can simulate fluid shear forces, better matching the growth environment of organoid cells. Currently, most organoid culture devices in laboratories are static culture devices in single culture boxes. Due to cost considerations, many laboratories do not have dynamic culture devices. Therefore, it is necessary to design a low-cost device that can achieve batch organoid culture. Utility Model Content

[0003] To address the shortcomings of the existing technology, this invention provides a low-cost device that can achieve mass organoid culture.

[0004] The technical solution adopted by this utility model to solve the above-mentioned technical problems is as follows: an organoid culture device, including a liquid storage box and a culture box, both of which are provided with a liquid inlet and a liquid outlet; it also includes a support, which includes several support platforms arranged vertically, and a peristaltic pump is provided on the support; the liquid storage box is placed on the top support platform, and the culture box is placed on the bottom support platform; the liquid outlet of the liquid storage box is connected in series with the liquid inlets of several culture boxes from top to bottom through a flexible tube, and the liquid outlets of several culture boxes are connected in series from top to bottom; the liquid outlet of the bottom culture box is connected to the liquid inlet of the liquid storage box through a return liquid flexible tube, and the return liquid flexible tube passes through the peristaltic pump.

[0005] Furthermore, the storage container is equipped with a ventilation valve. The ventilation valve is used to regulate the air pressure balance inside and outside the storage container. When culture medium is placed in the storage container, the ventilation valve is opened to control the culture boxes inside the storage container to enter the culture boxes in sequence, and then the ventilation valve is closed.

[0006] Furthermore, considering that this invention can be placed on a shaker to simulate lateral shearing forces during use, a limiting part is provided on the support platform to prevent displacement of the liquid storage box and culture box during left and right swinging. Even further, to facilitate fixing to the shaker chassis, a switch magnet is provided at the bottom of the bracket. The switch magnet is a small, manual switch magnet that can be attracted to the steel shaker chassis.

[0007] To enable real-time monitoring of the culture status, a sensor mounting port is further provided on the storage tank. A sensor for detecting the culture medium environment can be mounted through this port.

[0008] Furthermore, to facilitate the replacement of the culture medium, a three-way valve is installed at the outlet of the storage box. One outlet of the three-way valve is connected to the inlet of the culture box, while the other outlet is left unused. When the culture medium needs to be replaced, a drain tube can be inserted into this outlet.

[0009] The culture box structure of this utility model can adopt an existing culture box. Furthermore, the front and rear ends of the culture box are separated into culture medium temporary storage areas by semi-permeable membranes, and the inlet and outlet of the culture box are respectively connected to the culture medium temporary storage areas.

[0010] The beneficial effects of this invention are as follows: it can set up multiple culture boxes through a support frame, and with the peristaltic pump on the support frame, it can realize the dynamic circulation of the culture medium; the culture medium in the storage box flows through the series-connected culture boxes in sequence under the dual action of the peristaltic pump and gravity, and the culture medium flows smoothly without generating additional pressure; the storage box is equipped with a sensor mounting port, and the status of the culture medium can be monitored in real time through the sensor so as to replace it in time; moreover, this device can also be used for culture on a shaker to realize dynamic simulation in four directions: front, back, left, and right; the support platform is equipped with a limiting part, and the support frame is fixed on the shaker to maintain the stability of the culture box. Attached Figure Description

[0011] Appendix Figure 1 This is a schematic diagram of the support structure of this utility model; Appendix Figure 2 This is a schematic diagram showing the connection state of the liquid storage box and the culture box of this utility model; In the diagram: 1. Liquid storage box, 101. Liquid inlet, 102. Sensor mounting port, 103. Liquid injection port, 104. Air exchange valve, 105. Three-way valve, 106. 2. Culture box, 201. Liquid inlet, 202. Liquid injection port, 203. Semi-permeable membrane, 204. Culture medium temporary storage area, 205. Base, 3. Vertical rod, 4. Support platform, 5. Limiting part, 501. Peristaltic pump, 6. Return hose, 7. Switch magnet. Detailed Implementation

[0012] To better understand this utility model, the following description is in conjunction with the appendix. Figure 1 and 2 The embodiments of this utility model will be explained in detail below.

[0013] The organoid culture device of this utility model includes a liquid storage box 1, a culture box 2, and a support. Both the liquid storage box 1 and the culture box 2 are provided with a liquid inlet and a liquid outlet, specifically, as shown below... Figure 2In one embodiment of the liquid storage box 1 shown, a liquid inlet 101 is provided at the upper left and a liquid outlet 102 is provided at the lower right. Multiple sensor mounting ports 103 and a liquid injection port 104 are provided at the top of the liquid storage box 1. The culture box 2 has a liquid inlet 201 at the top and a liquid outlet 202 at the bottom. At least one injection port 203 is provided at the top of the culture box. Figure 2 As shown, two injection ports 203 are provided, which are also used for collecting cultured tissues.

[0014] The support frame includes a base 3, a vertical rod 4, and several support platforms 5 arranged vertically on the vertical rod 4. A peristaltic pump 6 is fixedly mounted on the support frame. Figure 1 As shown, the peristaltic pump 6 is fixed in the middle of the vertical rod 4. The liquid storage box 1 is placed on the top support platform 5, and the culture box 2 is placed on the lower support platform 5. The outlet of the liquid storage box 1 is connected in series with the inlets of several culture boxes 2 from top to bottom via a flexible tube. The outlets of several culture boxes 2 are connected in series from top to bottom. The outlet of the bottom culture box is connected to the inlet 101 of the liquid storage box 1 via a return hose 7, and the return hose 7 passes through the peristaltic pump 6. The return hose 7 is detachably locked in the groove of the peristaltic pump 6.

[0015] This invention further incorporates a venting valve 105 on the liquid storage box 1. The purpose of this valve is to slowly introduce the culture medium from the liquid storage box 1 into several culture boxes 2 via the venting valve 105. The venting valve 105 can be a bolt, with a threaded hole on the liquid storage box for bolt engagement. Specifically, this device, according to… Figure 2 After connecting the tubing, clamp the tubing at the outlet 102 of the storage box 1 with a clamp, and then inject the culture medium into the storage box 1 through the injection port 104. After covering the injection port 104, slowly open the ventilation valve 105. The culture medium in the storage box 1 slowly flows into several culture boxes in sequence under the action of gravity. When the culture medium flows to the outlet of the bottom culture box, close the ventilation valve 105 and turn on the peristaltic pump 6. The culture medium enters the circulation working state under the action of the peristaltic pump.

[0016] When the support of this invention is stationary, the culture medium in the culture box 2 moves in the front-to-back direction ( Figure 2 The fluid flows in the left and right directions, generating shear force. However, there is no dynamic simulation in the left and right directions. To simulate the dynamic environment in the left and right directions, the support can be placed on a shaker and slowly shaken left and right. At this time, it is necessary to ensure the stability of the storage box and culture box during the left and right shaking process. Therefore, a limiting part 501 is provided on the support platform 5. The limiting part 501 is preferably as follows... Figure 1 The four right-angle brackets shown are positioned precisely at the four corners of the liquid storage box or culture box.

[0017] When the bracket needs to be fixed to the shaker, the base 3 of the bracket can be fixed using the fasteners on the shaker chassis. For easy fixing and disassembly of the bracket, a switch magnet 8 is provided on the base 3. The switch magnet 8 is a manual switch magnet (existing product), allowing the base 3 to be fixed and separated from the shaker chassis via a manual switch. A through hole is made in the base 3, and the bottom of the switch magnet 8 passes through the through hole and is flush with the bottom surface of the base 3.

[0018] To facilitate the replacement of the culture medium in the storage tank 1, a three-way valve 106 is installed at the outlet of the storage tank 1. When it is necessary to drain the culture medium, a flexible hose is connected to the empty outlet of the three-way valve 106, and the other end of the hose is connected to the waste liquid tank. After connection, the switch of the three-way valve 106 is turned to drain the culture medium. Of course, those skilled in the art can design the three-way valve 106 as an electric valve as needed.

[0019] This utility model further designs a culture box, specifically, as follows: Figure 2 As shown, the front and rear ends of culture box 2 (here, "front and rear" refers to the direction of culture medium flow, from...) Figure 2 The culture box 2 (viewed from the left and right ends) is separated into culture medium storage areas 205 by semi-permeable membranes 204. The inlet and outlet of the culture box 2 are connected to the culture medium storage areas 205. The middle position of the culture box 2 is an inoculation plate, or an organoid culture chip. Of course, the culture box 2 of the present invention can be a conventional dynamic culture box. Those skilled in the art can also design and improve other culture boxes as needed.

[0020] In use, this invention involves first performing embedding and inoculation operations in a sterile environment within the culture box, then injecting culture medium into the storage box. The storage box and culture box are then connected with a tubing and placed on a support. The support is then placed in the incubator, and the peristaltic pump is turned on to begin culturing. If a shaking incubator is required, the base of the support is fixed to the shaking incubator chassis, and the shaking amplitude and frequency are adjusted.

[0021] It should be noted that although this specification describes the embodiments, not every embodiment contains only one independent technical solution. This way of describing the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An organoid culture device, comprising a liquid storage box and a culture box, wherein both the liquid storage box and the culture box are provided with a liquid inlet and a liquid outlet; characterized in that: It also includes a support frame, which includes several support platforms arranged vertically, and a peristaltic pump is installed on the support frame; the liquid storage box is placed on the top support platform, and the culture box is placed on the bottom support platform. The liquid outlet of the liquid storage box is connected in series with the liquid inlets of several culture boxes from top to bottom through a flexible tube, and the liquid outlets of several culture boxes are connected in series from top to bottom. The liquid outlet of the bottom culture box is connected to the liquid inlet of the liquid storage box through a return liquid tube, and the return liquid tube passes through the peristaltic pump.

2. The organoid culture device as described in claim 1, characterized in that: The liquid storage box is equipped with a ventilation valve.

3. The organoid culture device as described in claim 1, characterized in that: A limiting part is provided on the support platform.

4. The organoid culture device as described in claim 3, characterized in that: A switch magnet is provided at the bottom of the bracket.

5. The organoid culture device as described in claim 1, characterized in that: A sensor mounting port is provided on the liquid storage box.

6. The organoid culture device as described in claim 1, characterized in that: A three-way valve is installed at the outlet of the liquid storage box.

7. The organoid culture device as described in claim 1, characterized in that: The front and rear ends of the culture box are separated into culture medium temporary storage areas by semi-permeable membranes, and the inlet and outlet of the culture box are respectively connected to the culture medium temporary storage areas.

Images