Culture bag elastic fixing mechanism for shaking table

Abstract

The utility model discloses a culture bag elastic fixing mechanism for rocking bed, the fixing mechanism includes: cross column board, handle, spring, locking sleeve and pressboard, the cavity is equipped with in cross column board, and the side of cross column board is equipped with the entrance and exit that communicates with the cavity, handle is through the upper end of cross column board and stretches into the cavity, spring is sleeved on the handle in the cavity, locking sleeve is connected with the handle in the cavity, and locking sleeve and the inner wall sliding connection in the cavity, and the both ends of spring are respectively with cross column board and locking sleeve and meet, pressboard is located locking sleeve below, and pressboard and handle lower end detachable connection, the cavity is equipped with still the gusset plate, and the space for accommodating cell culture bag is equipped between gusset plate and pressboard, the utility model provides a culture bag elastic fixing mechanism for rocking bed, which has good fixing effect, convenient operation and can adapt to different specifications of cell culture bag.

Description

Technical Field

[0001] This utility model belongs to the field of cell culture equipment technology, specifically relating to an elastic fixing mechanism for culture bags used in shakers. Background Technology

[0002] A shaker is an experimental device used to provide gentle shaking or vibration when culturing cells. This shaking helps keep cells suspended in the culture medium and ensures that nutrients and oxygen are evenly distributed, promoting cell growth and health.

[0003] Currently, cell culture bags are fixed in a shaker by a clamping mechanism that presses the support rods on the cell culture bags to maintain their relative position. However, due to the high dimensional accuracy of the cell culture bags, and the inability of workers to precisely control this accuracy during the heat-sealing process, the support rods of the cell culture bags are sometimes difficult to press into the clamping mechanism, or even impossible. The cell culture bags then float in the air and cannot contact the shaker chassis, leading to erroneous detections by the temperature, pH, and DO sensors during the shaking process, and even the cell culture bags falling off due to the inertia of the shaking. Furthermore, the presence of metal components and untreated microburrs on the clamping mechanism can cause damage to the cell culture bags.

[0004] Patent 202322661901.2 discloses a WAVE reactor bag pressing mechanism. By using a rotating second screw to drive the pressure plate to descend, the pressure plate presses the edge of the cell culture bag tightly to achieve the purpose of fixing. After tightening the second screw, a fixing mechanism applies a pushing force to the positioning block, so that the positioning block is locked in the slot of the second screw, thereby preventing the second screw from reversing and loosening. However, because the shaker is in a constant vibration environment during the shaking process, it is easily subjected to continuous small displacements, which gradually weakens the fastening effect, causing the originally tight fit to become loose.

[0005] Therefore, there is an urgent need for an elastic fixation mechanism for culture bags used in shakers that provides good fixation, is easy to operate, and can adapt to cell culture bags of different sizes. Utility Model Content

[0006] To address the aforementioned technical problems and improve upon the shortcomings of the shaker's fixed culture mechanism, thereby facilitating better fit of cell culture bags of different sizes, this invention achieves its purpose through the following solution: An elastic fixing mechanism for a culture bag in a shaker, the fixing mechanism comprising: a crossbar, a handle, a spring, a locking sleeve, and a pressure plate; The horizontal panel has a cavity inside, and one side of the horizontal panel has an entrance / exit that communicates with the cavity. The handle passes through the upper end of the horizontal bar and extends into the cavity; the spring is sleeved on the handle inside the cavity; The locking sleeve is connected to the handle located in the cavity, and the locking sleeve is slidably connected to the inner side wall of the cavity. The two ends of the spring abut against the crossbar and the locking sleeve, respectively. The pressure plate is located below the locking sleeve, and the pressure plate is detachably connected to the lower end of the handle.

[0007] Furthermore, a pad is provided inside the cavity, and a space for accommodating cell culture bags is provided between the pad and the pressure plate.

[0008] Furthermore, the pad is located below the pressure plate to ensure the height of the cell culture bag.

[0009] Furthermore, the fixed culture mechanism also includes a locking screw, and the bottom of the pad is provided with a threaded hole corresponding to the locking screw. The locking screw passes through the cross plate and is threadedly connected to the threaded hole at the bottom of the pad.

[0010] Furthermore, an adjusting screw is threaded to the lower end of the crossbar, and the upper end of the adjusting screw abuts against the bottom of the pad.

[0011] Furthermore, the locking screws and adjusting screws form a pull-up structure on the pad, and the number of locking screws is greater than the number of adjusting screws.

[0012] Furthermore, locking screws are used to lock and fix the pads in place. When bearing a large weight or load, increasing the number of locking screws can improve the load-bearing capacity and stability of the entire structure.

[0013] Furthermore, the adjusting screws are used to adjust the height of the pad or fine-tune its position, while also providing more support points for the stability of the pad.

[0014] Preferably, the design using multiple locking screws and adjusting screws can evenly distribute the load, reduce the pressure on a single screw, and lower the risk of structural failure.

[0015] Furthermore, the upper end of the horizontal bar is provided with a gourd hole for the handle to pass through, the gourd hole including a large hole and a small hole that are connected.

[0016] Furthermore, the handle is inserted into the gourd hole and can switch between large and small holes.

[0017] Preferably, the gourd hole is arranged axially, which allows for adjustment of the position of the handle and spring within a certain range, and also allows for fine adjustment of the spring's preload.

[0018] Furthermore, the handle has a boss in the middle, the diameter of which is larger than the diameter of the small hole and smaller than the diameter of the large hole, and the diameter of the handle outside the boss is smaller than the diameter of the small hole.

[0019] Furthermore, when the handle outside the boss is moved horizontally to the upper end of the small hole, the spring is relaxed, and the operator can easily place the cell culture bag into any suitable position on the horizontal plate.

[0020] Furthermore, when the boss is moved horizontally to the upper end of the large hole and then vertically lowered, the spring is in a compressed state. The spring force is used to press and fix the support rod of the culture bag, preventing the handle from moving back and forth when the shaker is rocking.

[0021] Furthermore, the bottom of the crossbar is provided with a threaded hole, through which screws are passed to fix and connect to the tray assembly.

[0022] Furthermore, the position of the locking sleeve on the handle is adjustable.

[0023] Preferably, the locking sleeve guides the up-and-down movement of the handle.

[0024] Furthermore, the locking sleeve is connected to the handle via a set screw, and the spring force is adjusted by changing the position of the locking sleeve.

[0025] Preferably, since the space inside the crossbar is limited, when the locking sleeve and the handle are fixed by means of a set screw, the set screw occupies less space, which can fix the parts in the hole or provide a reliable fastening effect in the case of looseness. Moreover, the structure is simple, the operation is convenient, and it is easy to install and disassemble.

[0026] Furthermore, the lower end of the handle is provided with a threaded hole, and a screw passes through the pressure plate and is threadedly connected to the threaded hole at the lower end of the handle.

[0027] Preferably, the pressure plate is connected to the handle by a countersunk screw, which not only ensures that the pressure plate and the handle fit tightly together, but also allows the countersunk screw to be recessed below the surface of the component, maintaining the flatness of the pressure plate surface, reducing protrusions, and avoiding scratching the cell culture bag.

[0028] Furthermore, one end of the pressure plate is bent downwards.

[0029] Preferably, the downward-bending portion of the pressure plate can increase the contact area with the object being pressed, thus better distributing pressure; especially when there is a gap between the pressure plate and the object being pressed, it can help to better fix the cell culture bag.

[0030] When it is necessary to compress cell culture bags of different thicknesses, the downward-bending pressure plate can adapt to these changes, thereby accommodating the support rods of cell culture bags with different outer diameters and maintaining good contact and compression effect.

[0031] Furthermore, the edges of the pressure plate are rounded.

[0032] Furthermore, the fixed culture bag mechanism contains metal components, which may have untreated burrs and flash, which could easily cause the cell culture bag to be cut. In addition, to prevent the sharp edges of the pressure plate from potentially scratching the operators, the edges of the pressure plate are rounded.

[0033] Furthermore, the contact surface of the pressure plate is coated with polytetrafluoroethylene.

[0034] Preferably, polytetrafluoroethylene (PTFE) has non-adhesive properties and a very low coefficient of friction, which makes it difficult for cell culture bags to adhere to the surface of the pressure plate. At the same time, it can provide a low-friction interface between the pressure plate and the cell culture bag, reducing frictional resistance and wear during operation.

[0035] The cell culture bag used in this application is adapted to the elastic fixing mechanism of the culture bag for the shaker. The elastic fixing mechanism of the culture bag presses the support rod on the cell culture bag to ensure the relative position of the cell culture bag on the shaker.

[0036] Preferably, the support rods for cell culture bags of different sizes can be adapted by changing the pads.

[0037] Furthermore, the cell culture bag consists of a bag body, support rod, Luer male connector, silicone tube, cable tie, double-interface disc, clip, air filter, label, needleless connector, one-way valve, interface disc, electrode plate, DO patch and pH patch.

[0038] Preferably, the cell culture bag is made by heat sealing. Heat sealing may cause length deviations, but the elastic fixing mechanism of the culture bag can be adapted to cell culture bags of different specifications.

[0039] Preferably, within a certain range, the larger the volume of cells cultured in the cell culture bag, the larger the diameter of the support rod.

[0040] This invention relates to a culture bag elastic fixing mechanism for a shaker. The height of the pad is adjusted via a pull-up structure to accommodate support rods for cell culture bags of different sizes. The spring force compresses the support rods of the cell culture bag, thus securing the entire cell culture bag. The locking mechanism effectively prevents the cell culture bag from falling off during the shaking process, thus avoiding monitoring errors by the sensors.

[0041] Compared with the prior art, the beneficial effects of this utility model include: 1) This utility model provides an elastic fixing mechanism for culture bags for shakers, which can adapt to support rods of cell culture bags with different outer diameters and lengths, so that the entire cell culture bag is firmly fixed and the cell culture bag does not tilt during the shaking of the shaker. At the same time, it can better fit the cell culture bag to the heat-conducting plate and pull the sensor probe tighter, avoiding monitoring errors of temperature, pH and DO sensors.

[0042] 2) This utility model provides an elastic fixing mechanism for culture bags in a shaker, which can tighten or loosen the cell culture bag by lowering and raising the handle, making the whole operation process more convenient.

[0043] 3) This utility model provides an elastic fixing mechanism for culture bags in a shaker. The pad is designed with locking screws and adjusting screws to form a pull-top structure, which not only meets the pressing and fixing of support rods for cell culture bags of different sizes, but also allows for adjustment of the height of the center position of the cell culture bag.

[0044] 4) This utility model provides a culture bag elastic fixing mechanism for a shaker, which can not only adjust the spring force by changing the position of the locking sleeve, but also guide the up and down movement of the handle.

[0045] 5) This utility model provides an elastic fixing mechanism for culture bags in a shaker. By using a pressure plate coated with polytetrafluoroethylene and setting rounded corners at the edges of the pressure plate, damage to the cell culture bags can be avoided. Attached Figure Description

[0046] The present invention will be further described below with reference to the accompanying drawings and embodiments. In the accompanying drawings: Figure 1 This is a schematic diagram of the horizontal rail structure of the elastic fixing mechanism for the culture bag of this utility model; Figure 2 This is a partial cross-sectional view of the elastic fixing mechanism assembly for the culture bag of this utility model; Figure 3 This is a schematic diagram of the structure of the elastic fixing mechanism for the culture bag of this utility model, which compresses the cell culture bag. Figure 4 This is a schematic diagram of the structure of the cell culture bag of this utility model; Figure 5 This is a schematic diagram of the structure of the tray of this utility model; Figure 6 This is a schematic diagram of the handle structure of the elastic fixing mechanism for the culture bag of this utility model.

[0047] The reference numerals in the diagram are as follows: 1-Horizontal plate, 2-Handle, 3-Spring, 4-Locking sleeve, 5-Pressure plate, 6-Plate, 7-Setting screw, 8-Countersunk screw, 9-Hoop hole, 10-Pull screw, 1001 Fixing screw, 1002 Locking screw, 1003 Adjusting screw, 11-Cell culture bag, 12-Support rod, 13-Luer male connector, 14-Silicone tube, 15-Dual-interface disc, 16-Clamping plate, 17-Air filter, 18-Label, 19-Needleless connector, 20-One-way valve, 21-Interface disc, 22-Electrode plate, 23-DO patch, 24-PH patch, 25-Tray assembly. Detailed Implementation

[0048] The present invention will be further described below with reference to the accompanying drawings and embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation of the present invention. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of the present invention can be combined with each other.

[0049] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0050] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0051] To better understand the technical solution of this utility model, specific embodiments are selected below to illustrate the elastic fixing culture bag mechanism for the shaker of this utility model.

[0052] Example 1 The elastic fixing mechanism for the culture bag of the shaker provided in this embodiment includes a horizontal plate 1, a handle 2, a spring 3, a locking sleeve 4, and a pressure plate 5. The horizontal plate 1 has a cavity inside, and one side of the horizontal plate 1 has an inlet and outlet communicating with the cavity. The handle 2 passes through the upper end of the horizontal plate and extends into the cavity. The spring sleeve 3 is connected to the handle 2 inside the cavity. The locking sleeve 4 is connected to the handle 2 located inside the cavity, and the locking sleeve 4 is slidably connected to the inner side wall of the cavity. The two ends of the spring 3 abut against the horizontal plate 1 and the locking sleeve 4, respectively. The pressure plate 4 is located below the locking sleeve, and the pressure plate 5 is detachably connected to the lower end of the handle 2. The elastic force of the spring 3 can be adjusted by changing the position of the locking sleeve 4 (due to manufacturing errors, there is a very small gap between the locking sleeve and the inner side wall of the horizontal plate).

[0053] The elastic fixing mechanism for culture bags in a shaker provided by this utility model allows the operator to easily insert the cell culture bag from the inlet / outlet of the horizontal plate 1 and place it in any suitable position under the pressure plate 5 when the spring 3 is relaxed, without considering the influence of spring deformation and force changes. Then, the handle 2 moves downward, so that the spring 3 is under force and in a compressed state, thereby achieving the pressing and fixing of the cell culture bag by the pressure plate.

[0054] Example 2 The elastic fixing mechanism for culture bags in the shaker provided in this embodiment differs from that in Embodiment 1, and the similarities will not be repeated here.

[0055] like Figure 1 As shown, the upper end of the horizontal panel 1 is provided with a gourd hole 9 for the handle 2 to pass through, and the gourd hole 9 includes a large hole and a small hole that are connected to each other. Figure 6 As shown, the handle 2 has a boss in the middle. The diameter of the boss is larger than the diameter of the small hole and smaller than the diameter of the large hole. The diameter of the handle is smaller than the diameter of the small hole.

[0056] The culture bag elastic fixing mechanism for the shaker provided by this utility model allows the spring 3 to be relaxed when the handle 2 outside the boss is moved horizontally to the upper end of the small hole. The cell culture bag can be inserted from the inlet / outlet of the horizontal plate 1, and the operator can easily place the cell culture bag in any suitable position under the pressure plate 5. When the boss is moved horizontally to the upper end of the large hole and dropped vertically, the spring 3 is in a compressed state. The elastic force of the spring 3 is used to press and fix the culture bag 11, preventing the handle from moving back and forth when the shaker shakes, thus achieving the pressing and fixing of the cell culture bag. This makes the entire process of pressing or relaxing the spring more convenient.

[0057] Example 3 The elastic fixing mechanism for culture bags in the shaker provided in this embodiment differs from that in Embodiment 1, and the similarities will not be repeated here.

[0058] like Figure 4 As shown, in this embodiment, the cell culture bag is adapted to the fixed culture mechanism. The cell culture bag 11 includes a support rod 12, a Luer male connector 13, a silicone tube 14, a double-interface disc 15, a clip 16, an air filter 17, a needleless connector 19, a one-way valve 20, an interface disc 21, an electrode plate 22, a DO patch 23, and a pH patch 24.

[0059] like Figure 3 As shown, in this embodiment, a pad 6 is also provided inside the cavity, and a space for accommodating cell culture bags is provided between the pad 6 and the pressure plate 5; the fixed culture mechanism also includes a locking screw 1002, and the bottom of the pad 6 is provided with a threaded hole corresponding to the locking screw 1002. The locking screw 1002 passes through the cross plate 1 and is threadedly connected to the threaded hole at the bottom of the pad 6; an adjusting screw 1003 is threadedly connected to the lower end of the cross plate 1, and the upper end of the adjusting screw 1003 abuts against the bottom of the pad 6.

[0060] In this embodiment, the spring force of the spring 3 is adjusted by changing the position of the locking sleeve 4. The spring force of the spring 3 is used to press and fix the support rod 12 of the cell culture bag, so as to fix cell culture bags of different specifications.

[0061] like Figure 2 As shown, in this embodiment, the pull-up structure on the pad 6 uses four fixing screws 1001 to lock the crossbar 1 onto the tray 25; four locking screws 1002 connect and pull the pad 6, while two adjusting screws 1003 push against the pad 6. By tightening or loosening the locking screws 1002 and adjusting screws 1003 with a wrench or other tools, the height position of the pad 6 relative to the bottom surface of the crossbar 1 can be adjusted to accommodate cell culture bag support rods with different outer diameters.

[0062] The cell culture bag elastic fixing mechanism for a shaker provided by this utility model allows the cell culture bag to enter through the inlet / outlet of the crossbar 1 and be placed at any suitable position under the pressure plate 5 and on the pad 6 when the spring 3 is relaxed. The height of the pad 6 is then adjusted by the engagement of the pull screw with the pad 6 to accommodate support rods of different cell culture bag sizes. After the height of the pad 6 is determined, the handle 2 moves downwards, compressing the spring 3 and thus securing the cell culture bag. This mechanism satisfies the need for securing support rods of cell culture bags of different sizes and allows for adjustment of the center height of the cell culture bag.

[0063] Example 4 The elastic fixing mechanism for culture bags in the shaker provided in this embodiment differs from that in Embodiment 1, and will be described in detail below. The same principles apply. The details in this embodiment will not be repeated.

[0064] One end of the pressure plate 5 is bent downwards, and the edge of the pressure plate 5 is rounded. The locking sleeve 4 is fixed to the pressure plate 5 by a set screw 7. On handle 2, the spring force can be adjusted by changing the position of locking sleeve 4.

[0065] The culture bag elastic fixing mechanism for a shaker provided by this utility model has a locking sleeve 4 connected to the handle 2 via a set screw. With the spring 3 relaxed, the cell culture bag is inserted through the inlet / outlet of the horizontal plate 1 and placed at any suitable position under the pressure plate 5. Then, the handle 2 moves downwards, causing the spring 3 to be compressed, thus achieving the clamping and fixing of the cell culture bag. The locking sleeve 4 guides the up-and-down movement of the handle 2, maintaining good contact and clamping effect.

[0066] The following is a detailed procedure for using the elastic fixing mechanism of the culture bag for the shaker to press and fix the support rod of the cell culture bag: Before starting the culture, the cell culture bag is installed and secured: 1) First, the operator pulls the handle 2 vertically up and moves it horizontally to place it on the upper end of the gourd hole 9 of the horizontal plate 1. At this time, the spring 3 is in a relaxed state. 2) Then, confirm the direction of the cell culture bag support rod 12 and place the cell culture bag support rod 12 at any suitable position at the entrance and exit of the horizontal plate 1. 3) After the cell culture bag support rod is in the right position, when the handle 2 is moved horizontally to the large hole 9 of the gourd-shaped plate 1 and dropped vertically, the support rod 12 of the cell culture bag 11 is pressed and fixed by the elastic force of the spring 3. 4) After securing the cell culture bag, perform a thorough inspection. Once everything is in order, set the shaking speed, temperature, and other parameters of the shaker according to the needs of cell growth, and start the cell culture.

[0067] When the culture is complete, remove the cell culture bag: 1) First, pull the handle 2 vertically up and move it horizontally to place it on the upper end of the gourd hole 9 of the horizontal plate 1. The spring 3 is in a relaxed state, which prevents the handle 2 from falling. 2) Then, the operator can remove the support rod 12 of the cell culture bag from the entrance of the horizontal panel 1.

[0068] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural transformations made using the content of this utility model specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A culture bag elastic fixing mechanism for a shaker, characterized in that, The fixing mechanism includes: a crossbar, a handle, a spring, a locking sleeve, and a pressure plate; The horizontal panel has a cavity inside, and one side of the horizontal panel has an entrance / exit that communicates with the cavity. The handle passes through the upper end of the horizontal bar and extends into the cavity; the spring is sleeved on the handle inside the cavity; The locking sleeve is connected to the handle located in the cavity, and the locking sleeve is slidably connected to the inner side wall of the cavity. The two ends of the spring abut against the crossbar and the locking sleeve respectively. The pressure plate is located below the locking sleeve, and the pressure plate is detachably connected to the lower end of the handle.

2. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The cavity is also equipped with a pad, and there is a space between the pad and the pressure plate for accommodating the cell culture bag.

3. The elastic fixing mechanism for culture bags in a shaker according to claim 2, characterized in that, The fixed culture mechanism also includes a locking screw. The bottom of the pad is provided with a threaded hole corresponding to the locking screw. The locking screw passes through the cross plate and is threadedly connected to the threaded hole at the bottom of the pad. An adjusting screw is threadedly connected to the lower end of the cross plate, and the upper end of the adjusting screw abuts against the bottom of the pad.

4. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The upper end of the horizontal bar is provided with a gourd-shaped hole for the handle to pass through. The gourd-shaped hole includes a large hole and a small hole that are connected to each other. The handle is provided with a boss in the middle. The diameter of the boss is larger than the diameter of the small hole and smaller than the diameter of the large hole. The diameter of the handle is smaller than the diameter of the small hole.

5. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The bottom of the crossbar is provided with threaded holes, and screws pass through the bottom threaded holes to be fixedly connected to the tray assembly.

6. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The position of the locking sleeve on the handle is adjustable.

7. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The lower end of the handle is provided with a threaded hole, and the screw passes through the pressure plate and is threadedly connected to the threaded hole at the lower end of the handle.

8. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, One end of the pressure plate is bent downwards.

9. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The edges of the pressure plate are rounded.

10. The elastic fixing mechanism for culture bags in a shaker according to claim 1, characterized in that, The contact surface of the pressure plate is coated with polytetrafluoroethylene.

Images