A high-efficiency mixing device integrating sampling and double-reagent reaction

Abstract

The utility model relates to reagent detection technical field, concretely relates to a kind of integrated sampling and high-efficiency mixing device of double reagent reaction, including first container, second container and sampling head, the top of first container is pasted with first sealing film, the bottom of second container is pasted with second sealing film, the middle part of sampling head is axially set with the liquid passage that passes through its upper and lower ends, the bottom of liquid passage is fixed with the first puncture structure for puncturing first sealing film, the middle part of liquid passage is fixed with the second puncture structure for puncturing second first sealing film, second container is inserted in the top of liquid passage.The high-efficiency mixing device of the utility model is simple structure, it is very convenient to carry out efficient secondary reaction system, it is also very suitable for application in oil phase and water phase mixing system, oil phase and water phase are mixed only when using, can avoid oil phase and water phase early mixing, the situation of reagent performance, such as aggregation or crystallization precipitation, in storage process.

Description

Technical Field

[0001] This utility model relates to the field of reagent detection technology, specifically to a high-efficiency mixing device that integrates sampling and dual-reagent reaction. Background Technology

[0002] Existing tests require two reagents: the collected sample is first reacted with a first reagent, and then with a second reagent before testing can be performed. Both reagents need to be stored independently. Chinese utility model patent CN213632903U discloses a reagent device for secondary sample processing, comprising a first container and at least one second container. The first container holds a first reagent used for the first sample processing. The second container holds a second reagent used for the second sample processing. A first sealing film is provided at the top of the first container. A second sealing film is provided at the bottom of the first container. A puncture device is provided on the upper part of the inner wall of the second container. The bottom of the first container and the top of the second container are detachably connected. In a first state, the puncture device does not contact the second sealing film, and the first reagent in the first container and the second reagent in the second container are stored independently. In a second state, the puncture device contacts and punctures the second sealing film, and the first reagent in the first container and the second reagent in the second container are mixed. The device also includes a sampling apparatus, comprising a main body, a sampling nozzle, a sample holding channel, and a sample outlet. The sampling nozzle is also used to puncture a first sealing film, allowing the sample obtained to be delivered into a first container. The sample is then mixed with a first reagent in the first container to complete the first treatment, and the first-treated sample mixture is stored in the first container. When the first and second containers are connected, the sampling apparatus is inserted into the first container, and the puncturing device contacts and punctures the second sealing film. The first-treated sample mixture in the first container mixes with the second reagent in the second container to form a second-treated sample mixture. Inverting the sampling apparatus along with the first and second containers allows the second-treated sample mixture to flow out sequentially through the second container, the first container, the sampling nozzle, and the sample holding channel, exiting from the sample outlet. This patented reagent device has a simple and ingenious structure, facilitating efficient secondary staining. It is easy to operate, provides excellent cell staining results, and produces clearly defined cell characteristics after staining, making it suitable for cell analysis under bright-field conditions. However, the patent also has the following drawbacks: when the piercing device of the second container contacts and pierces the second sealing film of the first container, the sample mixture after the first treatment will remain between the piercing device and the first container, making the detection inaccurate; and the sample mixture after the second treatment flows out of the sample outlet through the second container, the first container, the sampling nozzle, and the sample holding channel in sequence, which will mix with the sample mixture remaining in the sampling nozzle and the sample mixture remaining in the first container, increasing the detection error. Summary of the Invention

[0003] In order to overcome the shortcomings and deficiencies of the existing technology, the purpose of this utility model is to provide a highly efficient mixing device that integrates sampling and dual-reagent reaction.

[0004] The purpose of this utility model is achieved through the following technical solution: an efficient mixing device integrating sampling and dual reagent reaction, comprising a first container for holding a first reagent, a second container for holding a second reagent, and a sampling head for collecting samples. The top of the first container is covered with a first sealing film, and the bottom of the second container is covered with a second sealing film. A liquid channel penetrating both the upper and lower ends is axially opened in the middle of the sampling head. A first puncture structure for piercing the first sealing film is fixed at the bottom of the liquid channel, and a second puncture structure for piercing the second first sealing film is fixed in the middle of the liquid channel. The second container is inserted into the top of the liquid channel.

[0005] In the first state, the first reagent in the first container and the second reagent in the second container are stored independently.

[0006] In the second state, the first puncture structure punctures the first sealing film of the first container, and the sample collected by the sampling head mixes with the first reagent in the first container;

[0007] In the third state, the second puncture mechanism punctures the second sealing film of the second container, and the second reagent in the second container flows into the first container through the liquid channel for mixing.

[0008] Furthermore, the sampling head includes an insertion part, a pressing part fixed to the top of the insertion part, and a retaining ring fixed between the insertion part and the pressing part. The outer diameter of the insertion part is smaller than the inner diameter of the first container. In the second state, the insertion part is inserted into the top of the first container, and the bottom surface of the retaining ring abuts against the top surface of the first container.

[0009] Furthermore, the pressing part includes a first arc-shaped pressing part and a second arc-shaped pressing part arranged opposite to each other, wherein the height of the first arc-shaped pressing part is greater than the height of the second arc-shaped pressing part.

[0010] Furthermore, the outer wall of the insertion part is fixed with a plurality of evenly spaced first protruding rings. In the second state, the outer surface of each first protruding ring abuts against the inner surface of the first container. The outer wall of the lower middle part of the second container is fixed with a plurality of evenly spaced second protruding rings. The outer surface of each second protruding ring abuts against the inner wall of the liquid channel.

[0011] Furthermore, the volume of the first container is greater than the volume of the second container.

[0012] Furthermore, the top of the first puncture structure extends upward along the liquid channel to the bottom of the second puncture structure, and the first puncture structure, the second puncture structure, and the sampling head are integrally formed.

[0013] Furthermore, the cross-sections of both the first and second puncture structures are Y-shaped or X-shaped. A slot is provided in the middle of the bottom of the first puncture structure, and a sampling tube is inserted into the slot, with the bottom of the sampling tube extending out of the slot.

[0014] Furthermore, a dropper is fixed to the bottom of the first container, and a dripping channel communicating with the inside of the first container is axially opened in the middle of the dropper. An end cap for sealing the dripping channel is detachably connected to the outside of the dropper.

[0015] Furthermore, the top of the end cap is provided with a receiving groove that mates with the dropper, the outer side wall of the dropper is provided with an external thread, and the receiving groove is provided with an internal thread that mates with the external thread, and the external thread and the internal thread are screwed together.

[0016] Furthermore, a liquid-stopping rod is provided at the bottom center of the receiving groove, with the top of the liquid-stopping rod inserted upward into the dripping channel; a sealing ring is fitted at the bottom of the liquid-stopping rod, and the sealing ring is sandwiched between the bottom inner wall of the receiving groove and the bottom surface of the drip nozzle.

[0017] The beneficial effects of this utility model are as follows: The high-efficiency mixing device of this utility model has a simple structure, which makes it very convenient to carry out efficient secondary reaction systems. It is also very suitable for use in oil and water phase mixing systems. The oil and water phases are mixed only when in use, which can avoid the situation where the oil and water phases are mixed too early and agglomeration or crystal precipitation during storage affects the performance of the reagents. Attached Figure Description

[0018] Figure 1 This is a perspective view of Embodiment 1 of this utility model.

[0019] Figure 2 This is an exploded perspective view of Embodiment 1 of this utility model.

[0020] Figure 3 This is a perspective view of the sampling head described in this utility model.

[0021] Figure 4 This is a perspective view of the sampling head described in this utility model from another angle.

[0022] Figure 5 This is a cross-sectional view of Embodiment 1 of the present invention in the first state.

[0023] Figure 6 This is a cross-sectional view of Embodiment 1 of the present invention in the second state.

[0024] Figure 7 This is a cross-sectional view of Embodiment 1 of this utility model in the third state.

[0025] Figure 8This is a perspective view of Embodiment 2 of this utility model.

[0026] Figure 9 This is an exploded perspective view of the first container and end cap of Embodiment 2 of this utility model.

[0027] Figure 10 This is a cross-sectional view of the first container and end cap in Embodiment 2 of this utility model.

[0028] The attached figures are labeled as follows: first container 1, first sealing film 11, dropper 12, external thread 121, drip channel 13, second container 2, second sealing film 21, second convex ring 22, sampling head 3, liquid channel 30, first puncture structure 31, slot 311, second puncture structure 32, insertion part 33, first convex ring 331, pressing part 34, first arc-shaped pressing part 341, second arc-shaped pressing part 342, retaining ring 35, sampling tube 4, end cap 5, receiving groove 51, internal thread 511, liquid stop rod 52, sealing ring 53. Detailed Implementation

[0029] To facilitate understanding by those skilled in the art, the following description is provided in conjunction with embodiments and appendices. Figure 1-10 The present invention will be further described below. The content mentioned in the embodiments is not intended to limit the present invention.

[0030] like Figure 1-7 The following is an embodiment of an efficient mixing device integrating sampling and dual-reagent reaction, comprising a first container 1 for holding a first reagent, a second container 2 for holding a second reagent, and a sampling head 3 for collecting samples. The top of the first container 1 is covered with a first sealing film 11, and the bottom of the second container 2 is covered with a second sealing film 21. The sampling head 3 has a liquid channel 30 axially extending through its upper and lower ends in the middle. The bottom of the liquid channel 30 is fixed with a first puncture structure 31 for puncturing the first sealing film 11, and the middle of the liquid channel 30 is fixed with a second puncture structure 32 for puncturing the second first sealing film 11. The second container 2 is inserted into the top of the liquid channel 30.

[0031] In the first state, the first reagent in the first container 1 and the second reagent in the second container 2 are stored independently.

[0032] In the second state, the first puncture structure 31 punctures the first sealing film 11 of the first container 1, and the sample in the sampling tube mixes with the first reagent in the first container 1.

[0033] In the third state, the second puncture mechanism punctures the second sealing film 21 of the second container 2, and the second reagent in the second container 2 flows into the first container 1 through the liquid channel 30 for mixing. The bottom of the first puncture structure 31 is detachably connected to a sampling tube 4 for collecting samples.

[0034] In this embodiment, the sampling head 3 includes an insertion part 33, a pressing part 34 fixed to the top of the insertion part 33, and a retaining ring 35 fixed between the insertion part 33 and the pressing part 34. The outer diameter of the insertion part 33 is smaller than the inner diameter of the first container 1. In the second state, the insertion part 33 is inserted into the top of the first container 1, and the bottom surface of the retaining ring 35 abuts against the top surface of the first container 1. The above structure facilitates the insertion of the sampling head 3 into the first container 1.

[0035] In this embodiment, the pressing part 34 includes a first arc-shaped pressing part 341 and a second arc-shaped pressing part 342 arranged opposite to each other, with the height of the first arc-shaped pressing part 341 being greater than the height of the second arc-shaped pressing part 342. When the sampling head 3 is inserted into the first container 1, the first arc-shaped pressing part 341 is pressed; when the second container 2, with its top inverted, is pressed until it is flush with the second arc-shaped pressing part 342, the second puncture structure 32 inside the sampling head 3 can puncture the second sealing film 21 of the second container 2, allowing the second reagent to flow into the first container 1 to form a mixture and carry out the corresponding reaction.

[0036] In this embodiment, the outer wall of the insertion part 33 is fixed with a plurality of evenly spaced first protruding rings 331. In the second state, the outer surface of each first protruding ring 331 abuts against the inner surface of the first container 1. The outer wall of the lower middle part of the second container 2 is fixed with a plurality of evenly spaced second protruding rings 22, the outer surface of each second protruding ring 22 abutting against the inner wall of the liquid channel 30. The first protruding rings 331 facilitate the insertion of the sampling head 3 into the first container 1, and the second protruding rings 22 facilitate the insertion of the second container 2 into the liquid channel 30 of the sampling head 3.

[0037] In this embodiment, the volume of the first container 1 is greater than the volume of the second container 2. Specifically, the first reagent can be an aqueous phase reagent or an oil phase reagent, and is the larger volume reagent in the two reagents; the second reagent can be an aqueous phase reagent or a lyophilized powder reagent, and is the smaller volume reagent in the two reagents.

[0038] In this embodiment, the top of the first puncture structure 31 extends upward along the liquid channel 30 to the bottom of the second puncture structure 32, and the first puncture structure 31, the second puncture structure 32, and the sampling head 3 are integrally formed. This structure reduces liquid residue.

[0039] In this embodiment, the cross-sections of both the first puncture structure 31 and the second puncture structure 32 are Y-shaped or X-shaped. The Y-shaped or X-shaped structure of the first puncture structure 31 and the second puncture structure 32 facilitates the formation of a liquid channel 30 within the sampling head 3. The Y-shaped or X-shaped structure of the second puncture structure 32 prevents the second sealing film 21 from tightly constricting the puncture structure after the membrane is broken, thus preventing the second reagent from fully flowing out into the first container 1.

[0040] The first puncture structure 31 has a slot 311 in the middle of its bottom. A sampling tube 4 is inserted into the slot 311, with the bottom of the sampling tube 4 extending out of the slot 311. The sampling tube 4 is used for sampling, such as collecting blood samples. Optionally, the sampling tube 4 can be a glass tube or a plastic tube. Specifically, the sampling tube 4 is a quantitative sampling tube, such as a micro-siphon glass tube, which can be 5μL or 10μL, or it can be modified to be a hydrophilic plastic tube that can be used for siphon sampling.

[0041] like Figure 8-10 The illustration shows a second embodiment of a highly efficient mixing device integrating sampling and dual-reagent reaction. This embodiment differs from the first embodiment in that: a dropper 12 is fixed to the bottom of the first container 1, and a dripping channel 13 communicating with the inside of the first container 1 is axially opened in the middle of the dropper 12. An end cap 5 for sealing the dripping channel 13 is detachably connected to the outside of the dropper 12. This structure allows the reaction solution to drip out.

[0042] In this embodiment, the top of the end cap 5 is provided with a receiving groove 51 that mates with the drip nozzle 12. The outer side wall of the drip nozzle 12 is provided with an external thread 121, and the receiving groove 51 is provided with an internal thread 511 that mates with the external thread 121. The external thread 121 and the internal thread 511 are screwed together. The above structure facilitates the installation and removal of the end cap 5.

[0043] In this embodiment, a stop rod 52 protrudes upward from the center of the bottom of the receiving groove 51, and the top of the stop rod 52 is inserted upward into the dripping channel 13; a sealing ring 53 is fitted onto the bottom of the stop rod 52, and the sealing ring 53 is sandwiched between the bottom inner wall of the receiving groove 51 and the bottom surface of the dropper 12. Specifically, sealing the dripping channel 13 of the first container 1 prevents the liquid in the dripping channel 13, i.e., the first reagent, from failing to react with the sample and the second reagent, and also prevents the unreacted first reagent from dripping first, thus leading to inaccurate test results.

[0044] When using the high-efficiency mixing device of this invention, after sampling is performed through the sampling tube 4 of the sampling head 3, the sampling head 3, together with the sampling tube 4, punctures the first sealing film 11 at the top of the first container 1 through the first puncture structure 31. The sample is then mixed with the first reagent in the first container 1 to complete the corresponding reaction. For example, blood samples undergo hemolysis with hemolysin in the first container 1, lysing red blood cells and releasing hemoglobin. Pressing down the inverted second container 2 at the top of the sampling head 3 allows the second puncture structure 32 inside the sampling head 3 to puncture the second sealing film 21 of the second container 2, allowing the second reagent to flow into the first container 1 to form a mixture and carry out the corresponding reaction. For example, if phenylboronic acid solution is filled into the second container 2, after the red blood cells in the blood sample in the first container 1 are completely lysed, pressing the second container 2 adds the phenylboronic acid solution to the first container 1. Benzylboronic acid specifically binds to glycated hemoglobin, thereby enabling the detection of glycated hemoglobin. Alternatively, a dropper 12 can be placed at the bottom of the first container 1 and sealed with an end cap 5. After the reaction is complete, the end cap 5 on the dropper 12 can be removed, and the final reaction solution can be dripped out and added to a chromatography apparatus for further operations.

[0045] The above embodiments are preferred implementations of this utility model. In addition, this utility model can also be implemented in other ways. Any obvious substitutions without departing from the concept of this utility model are within the protection scope of this utility model.

Claims

1. A highly efficient mixing device integrating sampling and dual-reagent reaction, comprising a first container for holding a first reagent, a second container for holding a second reagent, and a sampling head for collecting samples, wherein the top of the first container is covered with a first sealing film, and the bottom of the second container is covered with a second sealing film, characterized in that: The sampling head has an axially oriented liquid channel running through its upper and lower ends. The bottom of the liquid channel is fixed with a first puncture structure for piercing the first sealing film, and the middle of the liquid channel is fixed with a second puncture structure for piercing the second sealing film. The second container is inserted into the top of the liquid channel. In the first state, the first reagent in the first container and the second reagent in the second container are stored independently. In the second state, the first puncture structure punctures the first sealing film of the first container, and the sample collected by the sampling head mixes with the first reagent in the first container; In the third state, the second puncture mechanism punctures the second sealing film of the second container, and the second reagent in the second container flows into the first container through the liquid channel for mixing.

2. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 1, characterized in that: The sampling head includes an insertion part, a pressing part fixed to the top of the insertion part, and a retaining ring fixed between the insertion part and the pressing part. The outer diameter of the insertion part is smaller than the inner diameter of the first container. In the second state, the insertion part is inserted into the top of the first container, and the bottom surface of the retaining ring abuts against the top surface of the first container.

3. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 2, characterized in that: The pressing part includes a first arc-shaped pressing part and a second arc-shaped pressing part arranged opposite to each other, wherein the height of the first arc-shaped pressing part is greater than the height of the second arc-shaped pressing part.

4. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 2, characterized in that: The outer wall of the insertion part is fixed with a plurality of evenly spaced first protruding rings. In the second state, the outer surface of each first protruding ring abuts against the inner surface of the first container. The outer wall of the lower middle part of the second container is fixed with a plurality of evenly spaced second protruding rings. The outer surface of each second protruding ring abuts against the inner wall of the liquid channel.

5. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 1, characterized in that: The volume of the first container is greater than the volume of the second container.

6. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 1, characterized in that: The top of the first puncture structure extends upward along the liquid channel to the bottom of the second puncture structure, and the first puncture structure, the second puncture structure and the sampling head are integrally formed.

7. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 1, characterized in that: Both the first puncture structure and the second puncture structure have Y-shaped or X-shaped cross sections. The bottom center of the first puncture structure has a slot, into which a sampling tube is inserted, with the bottom of the sampling tube extending out of the slot.

8. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 1, characterized in that: The bottom of the first container is fixed with a dropper, and the middle of the dropper is axially provided with a dripping channel communicating with the inside of the first container. The outside of the dropper is detachably connected with an end cap for sealing the dripping channel.

9. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 8, characterized in that: The top of the end cap has a receiving groove that mates with the dropper nozzle. The outer wall of the dropper nozzle has an external thread, and the receiving groove has an internal thread that mates with the external thread. The external thread and the internal thread are screwed together.

10. The high-efficiency mixing device integrating sampling and dual-reagent reaction according to claim 9, characterized in that: A liquid stop rod protrudes upward from the center of the bottom of the receiving groove, and the top of the liquid stop rod is inserted upward into the dripping channel; a sealing ring is fitted at the bottom of the liquid stop rod, and the sealing ring is sandwiched between the bottom inner wall of the receiving groove and the bottom surface of the drip nozzle.

Images