A semi-automatic solid phase extraction instrument

Abstract

The utility model provides a kind of semi-automatic solid phase extraction instrument, comprising: solid phase extraction module support, its bottom is equipped with collection seat positioning plate, collection seat positioning plate is equipped with collection seat positioning groove to place sample collection seat loaded with multiple collection tubes, the top of collection seat positioning plate is erected with fixed seat positioning plate, fixed seat positioning plate is equipped with fixed seat positioning groove to place injection device;Lead screw motor, be located at the top of solid phase extraction module support, the output shaft of the bottom end of lead screw motor is placed in the inside of solid phase extraction module support, the bottom end of output shaft is fixedly connected sample injection advancing plate and sample injection advancing plate is located just above each injection tube;Switching power supply, be located on solid phase extraction module support;Function controller, be located on solid phase extraction module support. Advantageous effect is that the utility model can improve the consistency of sample pretreatment, guarantee recovery rate and the repeatability and stability of experimental result.

Description

Technical Field

[0001] This utility model relates to the technical field of solid phase extraction instruments, and more specifically, to a semi-automatic solid phase extraction instrument. Background Technology

[0002] Mass spectrometry (MS) is a highly sensitive and specific analytical technique that analyzes the chemical composition, structure, and relative abundance of substances by measuring the mass-to-charge ratio (m / z) of ions. With its characteristics of accurate qualitative analysis, sensitive quantitative analysis, and wide applicability, mass spectrometry has become an indispensable core tool in modern scientific research and industrial applications, covering multiple fields including life sciences and medical research, environmental monitoring, clinical diagnostics and precision medicine, and food safety. However, mass spectrometry detection is highly sensitive to the purity of the sample matrix, the concentration of the target analyte, and the content of interfering substances. Therefore, the quality of sample pretreatment directly affects the mass spectrometry detection results.

[0003] Solid phase extraction (SPE) is a highly efficient sample pretreatment technique that uses adsorption, partitioning, and ion exchange between the stationary phase and the target analyte to separate, purify, and enrich the analyte in complex matrices. This reduces matrix interference and improves the accuracy and sensitivity of subsequent analyses (such as chromatography and mass spectrometry). Its applications cover almost all analytical fields requiring sample pretreatment, and it is particularly irreplaceable in trace analysis and complex matrix samples (such as biological, food, and environmental samples).

[0004] Solid-phase extraction (SPE) is a commonly used sample pretreatment technique. For different target analytes, common methods include negative pressure extraction, centrifugation, and gravity extraction. For thermally unstable or easily damaged by shear forces (such as certain biomolecules and readily degradable pesticides / veterinary drugs), negative pressure extraction and centrifugation are unsuitable, requiring gravity extraction. The low-disturbance conditions of gravity extraction reduce degradation or structural damage, making it more suitable for pretreatment of such compounds. During gravity extraction, a syringe is often used to slowly inject the sample into the solid-phase extraction column and microporous membrane filter, allowing the sample solution to flow slowly through these components and achieve extraction separation. Currently, the injection of the sample into the extraction column is typically done manually using a syringe or other injector. This manual operation results in low efficiency, long processing time, low throughput, and inconsistent liquid flow rates, leading to poor recovery repeatability and unstable results. Summary of the Invention

[0005] The technical problem to be solved by this utility model is to improve the consistency of sample pretreatment and ensure the recovery rate and the repeatability and stability of experimental results. In order to overcome the defects of the above-mentioned prior art (or related technology), this utility model provides a semi-automatic solid phase extraction instrument.

[0006] This utility model provides a semi-automatic solid-phase extraction instrument, comprising: A solid-phase extraction module support is provided. The bottom of the solid-phase extraction module support is provided with a collection seat positioning plate. The collection seat positioning plate has a collection seat positioning groove for placing a sample collection seat loaded with multiple collection tubes. A fixing seat positioning plate is provided above the collection seat positioning plate. The fixing seat positioning plate has a fixing seat positioning groove for placing an injection device. The injection device is loaded with an injection structure consisting of an injection tube, a solid-phase extraction column, and a microporous membrane filter, in the same number of tubes as the collection tubes, arranged from top to bottom. Each microporous membrane filter is connected to the corresponding collection tube. A lead screw motor is located at the top of the solid phase extraction module support. The output shaft at the bottom of the lead screw motor passes through the outer wall of the top of the solid phase extraction module support and is placed inside the solid phase extraction module support. The bottom of the output shaft is fixedly connected to the sample injection push plate, and the sample injection push plate is located directly above each of the injection tubes. A switching power supply is mounted on the support of the solid phase extraction module. A function controller is mounted on the solid phase extraction module support and is electrically connected to the lead screw motor and the function controller. The function controller drives the lead screw motor to drive the sample feeding plate to squeeze the piston top of each injection tube, so as to transfer the sample in each injection tube through the solid phase extraction column and the microporous membrane filter to each collection tube.

[0007] Compared with existing technologies, the semi-automatic solid-phase extraction instrument proposed in this application has the following advantages: In this application, the injection tube, solid-phase extraction column, and microporous membrane filter are integrated into the injection device and fixed inside the solid-phase extraction module support. The microporous membrane filter is connected to the collection tube in the sample collection seat. The rotation direction and speed of the lead screw motor are controlled by the function controller, which drives the sample injection plate to move up and down to squeeze the piston top of the injection tube, slowly injecting the sample into the solid-phase extraction column. The sample is then filtered through the microporous membrane filter and collected and stored in the collection tube. This achieves precise and stable flow rate control, avoids errors caused by flow rate fluctuations in manual operation, significantly improves the consistency of sample pretreatment, and ensures the recovery rate and the repeatability and stability of experimental results.

[0008] In one possible implementation, the number of each of the collection tubes, injection tubes, solid-phase extraction columns, and microporous membrane filters is four.

[0009] In one possible implementation, a baffle plate is provided in the middle of the solid phase extraction module support and the baffle plate is located above the injection device. The baffle plate has a rectangular through hole to expose the piston tip of each injection tube.

[0010] Compared with existing technologies, the above technical solution can shorten the travel path of the sample injection plate in the entire injection process and improve injection efficiency by setting a baffle plate as an intermediate component in the middle of the solid phase extraction module support.

[0011] In one possible implementation, an opening is provided on the outer wall of either side of the baffle plate, and the opening is connected to the rectangular through hole.

[0012] Compared with the prior art, the above technical solution allows the bottom of the injection device to enter the solid phase extraction module support first, and then the syringe piston part at the top of the injection device can easily enter the rectangular through hole through the opening.

[0013] In one possible implementation, the sample feeding and pushing plate has multiple positioning holes, and the top of the blocking plate has multiple positioning rods, the same number as each positioning hole, which pass through each positioning hole. The top of each positioning rod is fixedly connected to the inner wall of the top of the solid phase extraction module support.

[0014] Compared with existing technologies, the above technical solution can enhance the connection stability of the sample injection plate by opening positioning holes on the sample injection plate and setting positioning rods on the blocking plate.

[0015] In one possible implementation, a positioning sensor is provided on the outer wall of the sample injection push plate, and a positioning sensor is provided in the middle of the solid phase extraction module support on the same side as the positioning sensor and the positioning sensor is located above the blocking plate. The function controller drives the lead screw motor to drive the sample injection push plate to squeeze the piston top of each injection tube until the positioning sensor detects the positioning sensor and then drives the lead screw motor to stop.

[0016] Compared with existing technologies, the above technical solution can use the positioning sensor to intelligently identify the positioning sensor plate as the basis for stopping the lead screw motor, thus avoiding excessive control of the lead screw motor.

[0017] In one possible implementation, the injection device includes: A solid-phase extraction column holder, wherein the top of the solid-phase extraction column holder is provided with multiple first positioning columns, and the solid-phase extraction column holder is provided with multiple first circular through holes for each of the microporous filter membranes to pass through. The syringe positioning base plate has multiple second positioning posts at its top end and is fixedly connected to the solid phase extraction column fixing seat through each of the first positioning posts. The syringe positioning base plate has multiple second circular through holes for each of the solid phase extraction columns and each of the microporous filter membranes to pass through. The syringe positioning top plate has its bottom end fixedly connected to the syringe positioning bottom plate through each of the second positioning posts. The syringe positioning top plate has multiple irregular holes for each of the injection tubes, each of the solid phase extraction columns and each of the microporous membrane filters to pass through.

[0018] Compared with the existing technology, the above technical solution can restrict the penetration of the injection tube, solid phase extraction column and microporous membrane filter through a three-layer design, and fix the injection tube, solid phase extraction column and microporous membrane filter in layers to ensure the stability of the overall injection device.

[0019] In one possible implementation, the bottom end of the solid phase extraction module support is provided with multiple support feet.

[0020] Compared with existing technologies, the above technical solution can enhance the solid phase extraction module support. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is an exploded view of the overall structure of this utility model; Figure 3 This is a schematic diagram of the injection device of this utility model; Figure 4 This diagram illustrates the positional significance of the positioning sensor and positioning sensor chip of this utility model. Explanation of reference numerals in the attached figures: 1. Solid-phase extraction module support; 2. Collection seat positioning plate; 3. Collection seat positioning groove; 4. Collection tube; 5. Sample collection seat; 6. Fixing seat positioning plate; 7. Fixing seat positioning groove; 8. Injection device; 9. Injection tube; 10. Solid-phase extraction column; 11. Microporous membrane filter; 12. Lead screw motor; 13. Sample injection push plate; 14. Switching power supply; 15. Function controller; 16. Baffle plate; 17. Rectangular through hole; 18. Opening; 19. Positioning rod; 20. Positioning sensor plate; 21. Positioning sensor; 22. Solid-phase extraction column fixing seat; 23. First positioning post; 24. First circular through hole; 25. Syringe positioning base plate; 26. Second positioning post; 27. Second circular through hole; 28. Syringe positioning top plate; 29. ​​Irregularly shaped hole; 30. Support foot. Detailed Implementation

[0022] First, those skilled in the art should understand that these embodiments are merely used to explain the technical principles of the embodiments of this application and are not intended to limit the scope of protection of the embodiments of this application. Those skilled in the art can make adjustments as needed to adapt to specific application scenarios.

[0023] The present application will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0024] See Figure 1 and Figure 2 This application discloses a semi-automatic solid-phase extraction (SPE) instrument, mainly including a solid-phase extraction module support 1, a lead screw motor 12, a switching power supply 14, and a function controller 15. The bottom of the solid-phase extraction module support 1 is provided with a collection seat positioning plate 2, which has a collection seat positioning groove 3 for placing a sample collection seat 5 loaded with multiple collection tubes 4. Above the collection seat positioning plate 2 is a fixing seat positioning plate 6, which has a fixing seat positioning groove 7 for placing an injection device 8. The injection device 8 contains an injection structure consisting of an injection tube 9, a solid-phase extraction column 10, and a microporous membrane filter 11, arranged from top to bottom, with the same number of collection tubes 4 as the collection tubes. Each microporous membrane filter 11 is connected to its corresponding collection tube 4. The lead screw motor 12 is located on the solid-phase extraction module support. At the top of the frame 1, the output shaft of the lead screw motor 12 passes through the outer wall of the top of the solid phase extraction module support 1 and is placed inside the solid phase extraction module support 1. The bottom end of the output shaft is fixedly connected to the sample injection push plate 13, and the sample injection push plate 13 is located directly above each injection tube 9. The switching power supply 14 is located on the solid phase extraction module support 1. The function controller 15 is located on the solid phase extraction module support 1 and is electrically connected to the lead screw motor 12 and the function controller 15 respectively. The function controller 15 drives the lead screw motor 12 to drive the sample injection push plate 13 to squeeze the piston top of each injection tube 9 so as to transfer the sample in each injection tube 9 through the solid phase extraction column 10 and the microporous membrane filter 11 to each collection tube 4. The lead screw motor 12 pushes the sample injection push plate 13 up and down, thereby controlling the speed at which the syringe pushes the sample.

[0025] See Figure 3The injection device 8 includes a solid-phase extraction column holder 22, a syringe positioning base plate 25, and a syringe positioning top plate 28. The top of the solid-phase extraction column holder 22 is provided with multiple first positioning posts 23, and the solid-phase extraction column holder 22 has multiple first circular through holes 24 for each microporous membrane filter 11 to pass through. The top of the syringe positioning base plate 25 is provided with multiple second positioning posts 26, and the bottom end of the syringe positioning base plate 25 is fixedly connected to the solid-phase extraction column holder 22 through each first positioning post 23. The syringe positioning base plate 25 has multiple second circular through holes 27 for each solid-phase extraction column 10 and each microporous membrane filter 11 to pass through. The bottom end of the syringe positioning top plate 28 is fixedly connected to the syringe positioning base plate 25 through each second positioning post 26, and the syringe positioning top plate 28 has multiple irregular holes 29 for each injection tube 9, each solid-phase extraction column 10, and each microporous membrane filter 11 to pass through.

[0026] In this embodiment, the second circular through hole 27 on the syringe positioning base plate 25 is used to fix the injection tube 9. The diameter of the second circular through hole 27 is larger than the size of the solid phase extraction column 10 and the microporous membrane filter 11 and smaller than the capacity tube diameter of the injection tube 9. The syringe positioning top plate 28 and the syringe positioning base plate 25 are connected by four or more second positioning posts 26, so that the distance between the syringe positioning top plate 28 and the syringe base plate is equal to the height of the injection tube 9 when closed. The first circular through hole 24 on the solid phase extraction column fixing seat 22 has a diameter smaller than the solid phase extraction column 10 and larger than the bottom outlet diameter of the solid phase extraction column 10 and larger than the bottom outlet diameter of the microporous membrane filter 11. It can fix the solid phase extraction column 10 and the microporous membrane filter 11 and connect to the collection tube 4. The solid phase extraction column fixing seat 22 is also connected to the syringe positioning base plate 25 above by four or more first positioning posts 23.

[0027] In this embodiment, the sample collection holder 5 has four circular grooves for fixing the collection tube 4. The collection tube 4 is not limited to containers such as 10ml centrifuge tubes, 5ml centrifuge tubes, 2ml centrifuge tubes, or sample bottles.

[0028] In this embodiment, the sample injection plate 13 has multiple positioning holes, and the top of the blocking plate 16 has multiple positioning rods 19, the same number as each positioning hole, which pass through each positioning hole. The top of each positioning rod 19 is fixedly connected to the inner wall of the top of the solid phase extraction module support 1. Furthermore, the sample injection plate 13 is a rectangular plate with four positioning rods 19 passing through its four corners, which can ensure that the sample injection plate 13 will not have any horizontal displacement when it moves up and down.

[0029] See Figure 4The outer wall of the sample injection push plate 13 is provided with a positioning sensor 20. The middle part of the solid phase extraction module support 1 is provided with a positioning sensor 21 on the same side as the positioning sensor 20 and the positioning sensor 21 is located above the blocking plate 16. The function controller 15 drives the lead screw motor 12 to drive the sample injection push plate 13 to squeeze the piston top of each injection tube 9 until the positioning sensor 21 detects the positioning sensor 20 and then drives the lead screw motor 12 to stop. Furthermore, a positioning sensor 21 is installed above and below the sample injection push plate 13 respectively. When the sample injection push plate 13 pushes the piston of the injection tube 9 up and down, it controls the reset position and the lowest position of the sample injection push plate 13.

[0030] In this embodiment, the function controller 15 can be a general switch controller or a high-voltage DC brushless controller or a central control computer. By controlling the rotation direction and speed of the lead screw motor 12, which is connected to the sample feeding push plate 13, the function controller 15 controls the position and working speed of the sample feeding push plate 13.

[0031] In this embodiment, a baffle plate 16 is provided in the middle of the solid phase extraction module support 1 and the baffle plate 16 is located above the injection device 8. A rectangular through hole 17 is provided on the baffle plate 16 so that the piston top of each injection tube 9 can be exposed. An opening 18 is provided on the outer wall of any one side of the baffle plate 16 and the opening 18 is connected to the rectangular through hole 17. A plurality of support feet 30 are provided at the bottom of the solid phase extraction module support 1.

[0032] In this embodiment, the pretreated sample is collected in the injection tube 9, and then the extraction consumables are connected together in the order of the injection tube 9 containing the sample, the solid phase extraction column 10, and the microporous membrane filter 11 to form an injection structure. The sample collection seat 5 with the collection tube 4 installed is placed on the collection seat positioning groove 3, and the injection device 8 bracket with the injection structure installed is installed in the solid phase extraction module bracket 1.

[0033] In this embodiment, after the switching power supply 14 is turned on, the function controller 15 controls the lead screw motor 12 to reset, so that the sample injection push plate 13 returns to its initial position. The function controller 15 is operated to make the lead screw motor 12 push the sample injection push plate 13 at a set pushing speed. The sample is squeezed and pushed into the solid phase extraction column 10 and the microporous membrane filter 11 at a set speed. Finally, the sample extraction pretreatment operation is completed. After the sample pretreatment is completed, the collection tube 4 is taken out for subsequent detection and processing.

[0034] In this embodiment, the sample is slowly injected into the solid-phase extraction column 10 via a semi-automatic control injection tube 9 or syringe, achieving precise and stable flow rate control. This avoids errors caused by flow rate fluctuations during manual operation, significantly improving the consistency of sample pretreatment and ensuring the recovery rate and the repeatability and stability of experimental results. Compared to purely manual operation, the equipment can automatically complete the key injection process, reducing the frequency and intensity of manual intervention. It is particularly suitable for batch water sample processing scenarios, effectively saving manpower and improving work efficiency. It is specifically applicable to the pretreatment of water samples in section 14.2.5.2 of GB / T 5750.10-2023 Standard Test Methods for Drinking Water, meeting the technical requirements of the standard for the pretreatment process, ensuring that the pretreatment process complies with the specifications, providing a reliable prerequisite for the accuracy of subsequent test results, and contributing to the standardization of drinking water safety testing. In summary, by integrating efficiency, stability, labor-saving, and standard compatibility, this equipment achieves a unity of standardized operation, reliable results, and high work efficiency in the pretreatment of drinking water samples, and has strong practical value.

[0035] In the description of this application, the references to terms such as "an embodiment," "some embodiments," "in this embodiment," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0036] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A semi-automatic solid-phase extraction instrument, characterized in that, include: A solid-phase extraction module support is provided. The bottom of the solid-phase extraction module support is provided with a collection seat positioning plate. The collection seat positioning plate has a collection seat positioning groove for placing a sample collection seat loaded with multiple collection tubes. A fixing seat positioning plate is provided above the collection seat positioning plate. The fixing seat positioning plate has a fixing seat positioning groove for placing an injection device. The injection device is loaded with an injection structure consisting of an injection tube, a solid-phase extraction column, and a microporous membrane filter, in the same number of tubes as the collection tubes, arranged from top to bottom. Each microporous membrane filter is connected to the corresponding collection tube. A lead screw motor is located at the top of the solid phase extraction module support. The output shaft at the bottom of the lead screw motor passes through the outer wall of the top of the solid phase extraction module support and is placed inside the solid phase extraction module support. The bottom of the output shaft is fixedly connected to the sample injection push plate, and the sample injection push plate is located directly above each of the injection tubes. A switching power supply is mounted on the support of the solid phase extraction module. A function controller is mounted on the solid phase extraction module support and is electrically connected to the lead screw motor and the function controller. The function controller drives the lead screw motor to drive the sample feeding plate to squeeze the piston top of each injection tube, so as to transfer the sample in each injection tube through the solid phase extraction column and the microporous membrane filter to each collection tube.

2. The semi-automatic solid-phase extraction apparatus according to claim 1, characterized in that, The number of each of the aforementioned collection tubes, injection tubes, solid-phase extraction columns, and microporous membrane filters is four.

3. The semi-automatic solid-phase extraction apparatus according to claim 1, characterized in that, A baffle plate is provided in the middle of the solid phase extraction module support and is located above the injection device. The baffle plate has a rectangular through hole to expose the piston top of each injection tube.

4. The semi-automatic solid-phase extraction apparatus according to claim 3, characterized in that, An opening is provided on the outer wall of any one side of the baffle plate, and the opening is connected to the rectangular through hole.

5. The semi-automatic solid-phase extraction apparatus according to claim 3, characterized in that, The sample feeding and pushing plate has multiple positioning holes, and the top of the blocking plate has multiple positioning rods, the same number as each positioning hole, which pass through each positioning hole. The top of each positioning rod is fixedly connected to the inner wall of the top of the solid phase extraction module support.

6. The semi-automatic solid-phase extraction apparatus according to claim 3, characterized in that, The outer wall of the sample injection push plate is provided with a positioning sensor plate. The middle part of the solid phase extraction module support is provided with a positioning sensor on the same side as the positioning sensor plate and the positioning sensor is located above the blocking plate. The function controller drives the lead screw motor to drive the sample injection push plate to squeeze the piston top of each injection tube until the positioning sensor detects the positioning sensor plate and then drives the lead screw motor to stop.

7. The semi-automatic solid-phase extraction apparatus according to claim 1, characterized in that, The injection device includes: A solid-phase extraction column holder, wherein the top of the solid-phase extraction column holder is provided with multiple first positioning columns, and the solid-phase extraction column holder is provided with multiple first circular through holes for each of the microporous filter membranes to pass through. The syringe positioning base plate has multiple second positioning posts at its top end and is fixedly connected to the solid phase extraction column fixing seat through each of the first positioning posts. The syringe positioning base plate has multiple second circular through holes for each of the solid phase extraction columns and each of the microporous filter membranes to pass through. The syringe positioning top plate has its bottom end fixedly connected to the syringe positioning bottom plate through each of the second positioning posts. The syringe positioning top plate has multiple irregular holes for each of the injection tubes, each of the solid phase extraction columns and each of the microporous membrane filters to pass through.

8. The semi-automatic solid-phase extraction apparatus according to claim 1, characterized in that, The solid phase extraction module support is provided with multiple support feet at the bottom.

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