Liquid mixing device, sampler and flow cytometer

Abstract

The utility model discloses a kind of liquid mixing device, sampling instrument and flow cytometer. Liquid mixing device includes: rack;Sampling needle, sampling needle is located in rack;Vibration mechanism, including vibrating member and vibrating seat, vibrating member is located in rack and vibrating member is connected with vibrating seat, vibrating member is used to drive vibrating seat vibration;Vibrating seat is equipped with first through cavity for sampling needle to be arranged along up-down direction, the lateral wall of vibrating seat is equipped with at least one first lateral hole, one end of first lateral hole is communicated with first through cavity;Vibrating seat is configured to apply radial clamping force to sampling needle in first through cavity by first elastic member arranged in first lateral hole, so that sampling needle is elastically shaken in first through cavity under the action of vibrating member. In this way, the vibration transmission efficiency is improved by flexible impact for sampling needle, and the mixing effect of sample is enhanced.

Description

Technical Field

[0001] This utility model relates to the field of biomedical testing equipment technology, and in particular to a liquid mixing device, a sampler, and a flow cytometer. Background Technology

[0002] Test tubes placed on the flow cytometer are mounted on the instrument via a tube rack. When taking samples or adding new liquid samples, the liquid in the test tubes needs to be mixed. Currently, flow cytometers have several mixing methods, including manual mixing and equipment mixing. Manual mixing involves removing the test tubes and mixing them with a vortex mixer before placing them back on the tube rack. This mixing method has very low efficiency and poor mixing consistency. Equipment mixing involves stirring with a sampling needle, but existing methods for pushing and stirring with the sampling needle are not ideal, requiring high strength from the sampling needle material and resulting in poor sample mixing. Utility Model Content

[0003] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a liquid mixing device that improves the vibration transmission efficiency and enhances the mixing effect of the sample by using flexible impact on the sampling needle.

[0004] This utility model further proposes a sampling instrument.

[0005] This invention further proposes a flow cytometer.

[0006] A liquid mixing device according to a first aspect of the present invention includes: a frame; a sampling needle disposed on the frame; a vibration mechanism including a vibrating element and a vibration seat, the vibrating element being disposed on the frame and connected to the vibration seat, the vibrating element being used to drive the vibration seat to vibrate; the vibration seat having a first through cavity for the sampling needle to pass through in a vertical direction, and the side wall of the vibration seat having at least one first transverse hole, one end of the first transverse hole communicating with the first through cavity; the vibration seat being configured to apply a radial clamping force to the sampling needle in the first through cavity through a first elastic element passing through the first transverse hole, so that the sampling needle elastically oscillates in the first through cavity under the action of the vibrating element.

[0007] Therefore, this embodiment uses a flexible impact on the sampling needle. Compared with the traditional rigid impact method, during the vibration process, the sampling needle and the vibration seat form a flexible impact, so that the sampling needle forms a gentle and high-frequency impact on the sample liquid in the test tube. This can achieve higher vibration response sensitivity and greater vibration amplitude, and improve the homogenization of the sample.

[0008] According to some embodiments of the present invention, the sampling needle ring is provided with a first boss; the first through cavity includes a first cavity segment and a second cavity segment connected in the vertical direction, the inner diameter of the first cavity segment is larger than the inner diameter of the second cavity segment, the first transverse hole is connected to the first cavity segment, the inner diameter of the first boss is adapted to the outer diameter of the first cavity segment, a gap is provided between the sampling needle and the second cavity segment, and the vibration seat is configured such that the first elastic element passes through the first transverse hole to apply a radial clamping force to the first boss in the first cavity segment.

[0009] According to some embodiments of the present invention, there are multiple first transverse holes, which are evenly distributed circumferentially on the sidewall of the vibration seat, and each first transverse hole is provided with the first elastic element.

[0010] According to some embodiments of the present invention, the sampling needle ring is provided with a second protrusion, and the side wall of the second protrusion is provided with a plurality of second transverse holes; the frame is provided with a fixed seat, the fixed seat is provided with a second through cavity for the sampling needle to pass through in the vertical direction and a gap is provided between the second through cavity and the sampling needle, the side wall of the fixed seat is provided with a plurality of third transverse holes, one end of the third transverse hole is connected to the second through cavity; the fixed seat is configured to apply a radial abutment force to the inner wall of the second transverse hole through a second elastic element passing through the third transverse hole.

[0011] According to some embodiments of the present invention, the first elastic member and / or the second elastic member includes: a fastening portion and an elastic portion connected together, wherein the fastening portion of the first elastic member is fastened to the first transverse hole and its elastic portion applies a radial clamping force to the sampling needle, and the fastening portion of the second elastic member is fastened to the third transverse hole and its elastic portion penetrates the second transverse hole and abuts against the inner wall of the hole.

[0012] According to some embodiments of the present invention, the frame is provided with a first support and the first support extends laterally relative to the frame, and the fixing seat is fixed to the end of the first support away from the frame.

[0013] According to some embodiments of this utility model, it further includes: a first moving mechanism, which is disposed on the frame and drivenly connected to the vibration mechanism, the first moving mechanism being used to drive the vibration mechanism to move in the up-down direction; the vibration seat is provided with a liquid passage communicating with the outside, the liquid passage communicating with the first through cavity, and the vibration seat is configured to move up and down by the first moving mechanism, and the cleaning fluid is input and extracted from the liquid passage.

[0014] According to some embodiments of the present invention, the first moving mechanism includes: a first driving member, a guide rail, and a transmission assembly. The first driving member, the guide rail, and the transmission assembly are all disposed on the frame. The transmission assembly is connected to the vibration mechanism. The first driving member is used to drive the transmission assembly to move. The transmission assembly drives the vibration mechanism to move up and down along the guide rail, so as to move the vibration seat up and down.

[0015] According to some embodiments of the present invention, the vibrating element includes: a second driving element and a hollow cup swinging element, the hollow cup swinging element being connected to the output end of the second driving element; and / or the frame is provided with a second support and the second support extends laterally relative to the frame, the vibrating element being connected to the second support, and the vibrating seat being connected to the second support.

[0016] A sampling device according to a second aspect of the present invention includes: a base plate; a second moving mechanism disposed on the base plate, the second moving mechanism being connected to a test tube placement plate, the second moving mechanism being used to drive the test tube placement plate to move in the up-down direction, the left-right direction, and the front-back direction; and the liquid mixing device, the device being mounted on the base plate, the test tube placement plate being movable to a position below the sampling needle so that the sampling needle can take samples from test tubes on the test tube placement plate.

[0017] The flow cytometer according to a third aspect of the present invention includes the aforementioned sampling device.

[0018] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0019] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which: Figure 1 This is a schematic diagram of the structure of the sampler including a liquid mixing device according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the structure of a liquid mixing device according to an embodiment of the present utility model; Figure 3 This is a schematic diagram of the structure of the vibration seat and the fixed seat respectively cooperating with the sampling needle according to an embodiment of the present utility model; Figure 4 This is an exploded view of the vibration seat and the fixed seat respectively cooperating with the sampling needle according to an embodiment of the present utility model; Figure 5 This is a cross-sectional schematic diagram showing the vibration seat and the fixed seat respectively cooperating with the sampling needle according to an embodiment of the present utility model.

[0020] Figure label: S. Sampling instrument; 100. Liquid mixing device; 1. Frame; 11. First support; 12. Fixing plate; 13. Second support; 2. Sampling needle; 3. Vibrating component; 31. Second driving component; 32. Hollow cup ejector component; 4. Vibrating seat; 41. First through cavity; 4101. First cavity section; 4102. Second cavity section; 42. First transverse hole; 43. First elastic element; 44. Liquid passage; 5. First boss; 6. Second boss; 61. Second transverse hole; 7. Fixed base; 71. Second through cavity; 72. Third transverse hole; 73. Second elastic element; 8. First moving mechanism; 81. First driving component; 82. Guide rail; 83. Transmission assembly; 8301. Transmission wheel; 8302. Transmission belt; 91. Fastening part; 92. Elastic part; 10. Optocoupler; 200, base plate; 300, second moving mechanism; 400, test tube placement plate. Detailed Implementation

[0021] The embodiments of the present invention are described in detail below. The embodiments described with reference to the accompanying drawings are exemplary. The embodiments of the present invention are described in detail below.

[0022] The following is for reference. Figures 1-5 The present invention describes a liquid mixing apparatus 100, a sampler S, and a flow cytometer according to embodiments of the present invention.

[0023] like Figures 1-2 As shown, the liquid mixing device 100 includes a frame 1, a sampling needle 2, and a vibration mechanism. The sampling needle 2 is mounted on the frame 1. The vibration mechanism includes a vibrating element 3 and a vibrating seat 4. The vibrating element 3 is mounted on the frame 1 and connected to the vibrating seat 4, and the vibrating element 3 drives the vibrating seat 4 to vibrate. The vibrating seat 4 has a first through cavity 41 through which the sampling needle 2 passes in the vertical direction. The side wall of the vibrating seat 4 has at least one first transverse hole 42, one end of which communicates with the first through cavity 41. The vibrating seat 4 is configured to apply a radial clamping force to the sampling needle 2 in the first through cavity 41 through a first elastic element 43 passing through the first transverse hole 42, so that the sampling needle 2 elastically oscillates in the first through cavity 41 under the action of the vibrating element 3.

[0024] When in use, the sampling needle 2 is inserted into the test tube. Before the sampling needle 2 samples the sample in the test tube, the vibrating element 3 is activated. The vibrating element 3 drives the vibrating seat 4 to vibrate, and the vibrating seat 4 drives the sampling needle 2 to vibrate synchronously. The vibrating sampling needle 2 stirs and mixes the sample in the test tube. After stirring, the sampling needle 2 draws aspiration to sample the mixed sample in the test tube.

[0025] The first elastic element 43, which passes through the first transverse hole 42, applies a radial clamping force to the sampling needle 2, which passes through the first through cavity 41, so that the sampling needle 2 elastically wobbles in the first through cavity 41 under the action of the vibrating element 3.

[0026] With this configuration, the sampling needle 2 is effectively positioned while forming a flexible elastic contact with the vibration seat 4. When the vibrating element 3 drives the vibration seat 4 to generate high-frequency reciprocating vibration, the sampling needle 2, under elastic constraint, does not rigidly follow the movement but can undergo controlled elastic swaying or swinging within a controllable gap range. During this vibration process, periodic flexible impacts are generated between the sampling needle 2 and the vibration seat 4, resulting in periodic flexible impacts and disturbances between the sampling needle 2 and the liquid sample it is in. On the one hand, the sampling needle 2 forms a flexible impact with the first elastic element 43, avoiding needle damage or stress concentration caused by rigid impacts; on the other hand, the sampling needle 2 forms a gentle but high-frequency impact on the sample liquid in the test tube, and its own micro-vibration can excite eddies and shear flows inside the liquid. This non-rigid, low-damage, high-frequency dynamic disturbance mechanism formed by elastic contact can achieve higher vibration response sensitivity and a larger effective vibration amplitude, significantly enhancing the mixing uniformity and reaction efficiency of the liquid sample.

[0027] Compared to the traditional rigid fixing method where the sampling needle 2 only undergoes overall translational vibration, this solution introduces additional degrees of freedom and energy dissipation paths through an elastic clamping structure. This not only improves the smoothness and repeatability of the mixing process, making it particularly suitable for the high-efficiency mixing requirements of samples, but also effectively avoids needle damage or stress concentration caused by rigid collisions.

[0028] Therefore, this solution introduces an elastic clamping structure to create a flexible elastic contact between the sampling needle 2 and the vibration seat 4. During vibration, a flexible impact is formed between the sampling needle 2 and the vibration seat 4, so that the sampling needle 2 forms a gentle but high-frequency impact on the sample liquid in the test tube. This not only achieves higher vibration response sensitivity and a larger vibration amplitude, significantly enhancing the mixing uniformity of the liquid sample, but also avoids needle damage or stress concentration caused by rigid impact.

[0029] According to some embodiments of the present invention, there are multiple first transverse holes 42, which are evenly distributed around the side wall of the vibrating seat 4, and each first transverse hole 42 is provided with a first elastic element 43.

[0030] Specifically, multiple first transverse holes 42 are distributed in different positions on the side wall of the vibration seat 4, preferably symmetrically arranged, so that multiple first elastic elements 43 can apply a balanced elastic clamping force to the sampling needle 2 in different directions, and abut against the sampling needle 2 at multiple points to ensure its circumferential positioning stability and prevent the sampling needle 2 from rotating. At the same time, since each first elastic element 43 acts independently, the overall clamping stiffness is moderate, neither loose nor too tight, realizing "elasticity in constraint and flexibility in fixation", so that the sampling needle 2 can be effectively driven by multiple vibrations during vibration, further improving vibration efficiency.

[0031] According to some embodiments of this utility model, such as Figures 3-5 As shown, the sampling needle 2 is provided with a first protrusion 5. The first cavity 41 includes a first cavity segment 4101 and a second cavity segment 4102 connected in the vertical direction. The inner diameter of the first cavity segment 4101 is larger than the inner diameter of the second cavity segment 4102. The first transverse hole 42 is connected to the first cavity segment 4101. The inner diameter of the first protrusion 5 is adapted to the outer diameter of the first cavity segment 4101. A gap is provided between the sampling needle 2 and the second cavity segment 4102. The vibration seat 4 is configured such that the first elastic element 43 passes through the first transverse hole 42 and applies a radial clamping force to the first protrusion 5 in the first cavity segment 4101.

[0032] By setting a stepped first cavity 41 within the vibration seat 4, the first protrusion 5 can be embedded into the large-diameter first cavity section 4101 for axial positioning. A first elastic element 43, passing through the first transverse hole 42, applies a radial clamping force to the first protrusion 5 on the outer side of the sampling needle 2, causing a flexible impact between the first protrusion 5 and the vibration seat 4. The compression and rebound of the first elastic element 43 generate minute deformation, allowing the sampling needle 2 to obtain a controllable dynamic movement space. This creates a gentle but high-frequency impact on the sample liquid in the test tube, increasing the vibration amplitude and thus improving the sample mixing effect. Furthermore, the gap design between the sampling needle 2 and the second cavity section 4102 reduces motion interference of the sampling needle 2, preventing the large vibration amplitude of the sampling needle 2 from impacting the inner wall of the second cavity section 4102, and reducing the risk of failure of the sampling needle 2.

[0033] According to some embodiments of this utility model, such as Figures 3-5 As shown, the sampling needle 2 is provided with a second protrusion 6, and the side wall of the second protrusion 6 is provided with multiple second transverse holes 61. A fixing seat 7 is provided on the frame 1. The fixing seat 7 has a second through cavity 71 through which the sampling needle 2 passes in the vertical direction, and a gap is provided between the second through cavity 71 and the sampling needle 2. The side wall of the fixing seat 7 is provided with multiple third transverse holes 72, one end of which communicates with the second through cavity 71. The fixing seat 7 is configured to apply a radial abutment force to the inner wall of the second transverse hole 61 through a second elastic element 73 passing through the third transverse hole 72.

[0034] By adding a second protrusion 6 to the sampling needle 2 and providing a third transverse hole 72 and a second elastic element 73 on the fixed seat 7 of the frame 1 to cooperate with it, flexible radial fixation of the distal end of the sampling needle 2 is achieved. When the vibration mechanism drives the vibration seat 4 to vibrate the sampling needle 2, the second elastic element 73 applies a controllable radial abutment force to the inner wall of the second transverse hole 61 on the second protrusion 6. While ensuring reliable fixation of the sampling needle 2, the gap between the second through cavity 71 and the sampling needle 2 allows the sampling needle 2 to have a certain dynamic movement space, which can better improve vibration response and dynamic stability, thereby better improving the sample mixing effect.

[0035] It should be noted that the distal end of sampling needle 2 refers to the end of sampling needle 2 that is far away from the sample to be tested, while the proximal end of sampling needle 2 refers to the end of sampling needle 2 that draws the sample to be tested.

[0036] By flexibly fixing the distal end of the sampling needle 2, compared with the traditional rigid constraint structure, this embodiment effectively avoids needle damage or stress concentration that may be caused by rigid clamping, and realizes the flexible fixed connection of the sampling needle 2.

[0037] Furthermore, multiple second transverse holes 61 are distributed in the circumferential direction, and together with the second elastic element 73 in multiple third transverse holes 72, they can form a balanced radial support force in different directions, ensuring that the sampling needle 2 always remains in a centered state, preventing uneven wear or unilateral force, extending service life, and at the same time better improving the mixing effect of the sample to be tested.

[0038] According to some embodiments of the present invention, the first elastic member 43 and / or the second elastic member 73 include: a fastening part 91 and an elastic part 92 connected to each other. The fastening part 91 of the first elastic member 43 is fastened to the first transverse hole 42, and its elastic part 92 applies a radial clamping force to the sampling needle 2. The fastening part 91 of the second elastic member 73 is fastened to the third transverse hole 72, and its elastic part 92 passes through the second transverse hole 61 and abuts against the inner wall of the hole.

[0039] Specifically, the fastening part 91 is used to reliably connect (e.g., with a threaded fit) to the first transverse hole 42 or the third transverse hole 72, ensuring that the first elastic element 43 or the second elastic element 73 does not undergo axial displacement during vibration. The elastic part 92 extends inward and acts on the side wall of the first boss 5 or the inner wall of the second transverse hole 61 on the sampling needle 2, providing a controllable radial clamping force or abutment force. On the one hand, the fastening part 91 achieves a secure assembly of the first elastic element 43 or the second elastic element 73, preventing loosening due to vibration. On the other hand, the elastic part 92 can automatically compensate for minor deviations according to the force state, maintaining a continuous and stable contact pressure and improving the reliability of long-term use.

[0040] In some embodiments, when the fastening part 91 has a threaded structure, its insertion depth can be adjusted by rotation, thereby changing the compression amount of the elastic part 92 and achieving stepless adjustment of the clamping force or abutment force. This adjustable mechanism allows the device to be adapted to sampling needles 2 with different outer diameters or stiffnesses, enhancing the versatility and ease of maintenance of the device.

[0041] In addition, a ball head or an arc-shaped pressure head can be provided at the end of the elastic part 92 to reduce stress concentration, prevent damage to the surface of the boss, and extend service life.

[0042] refer to Figure 4 In the illustrated embodiment, the sampling needle 2 and the fixed base 7 are connected by a flexible fastening structure consisting of screws (fastening part 91) and springs (elastic part 92). There are a total of six such structures, arranged in three groups of two, one above the other, evenly distributed on the side of the fixed base 7. The sampling needle 2 and the vibrating base 4 are fastened by spring plungers, with three spring plungers evenly distributed on the side of the vibrating base 4.

[0043] According to some embodiments of the present invention, the frame 1 is provided with a first support 11, and the first support 11 extends laterally relative to the frame 1, and the fixing seat 7 is fixed to the end of the first support 11 away from the frame 1.

[0044] like Figure 2 As shown, a first support 11 extending laterally is provided on the frame 1, and the fixing seat 7 is fixed to the end of the first support 11 away from the frame 1, which not only achieves stable structural support, but also facilitates disassembly and maintenance. Compared with the traditional method of directly screwing the fixing seat 7 to the frame 1, this structure can set the sampling needle 2 at a position away from the frame 1, which is conducive to adjusting the position of several samples to be tested below the sampling needle 2.

[0045] In addition, a fixing plate 12 is provided at the end of the first bracket 11 away from the frame 1. The fixing plate 12 is connected to the first bracket 11 and a through installation space is defined between them in the vertical direction. The fixing seat 7 is limited within the installation space.

[0046] Since the installation space is completely open in the vertical direction, the sampling needle 2 can pass smoothly through the fixing seat 7 without any axial obstruction. Preferably, the fixing plate 12 and the first bracket 11 are connected by a detachable connection (such as screws, clips, etc.).

[0047] According to some embodiments of this utility model, the liquid mixing device 100 further includes: a first moving mechanism 8, which is mounted on the frame 1 and is drivenly connected to the vibration mechanism. The first moving mechanism 8 is used to drive the vibration mechanism to move in the up-down direction. The vibration seat 4 is provided with a liquid passage 44 communicating with the outside. The liquid passage 44 is connected to the first through cavity 41. The vibration seat 4 is configured to move up and down driven by the first moving mechanism 8, and the cleaning liquid is input and extracted from the liquid passage 44.

[0048] like Figure 2 As shown, a liquid passage 44 communicating with the outside is provided inside the vibration seat 4 and connected to the first through cavity 41, so that the cleaning fluid can be directly introduced into the gap area between the sampling needle 2 and the vibration seat 4. With the first moving mechanism 8 driving the vibration seat 4 to reciprocate in the up and down direction, the cleaning fluid can form a flow scouring effect on the outer wall of the sampling needle 2, effectively removing droplets or contaminants attached to the sample to be tested. When the vibration seat 4 moves downward to the limit, it will stay for a short time. At this time, the cleaning fluid will not stop flowing, and the sampling needle 2 will be sucked in to achieve the effect of cleaning its inner wall, which significantly reduces the risk of cross-contamination between samples.

[0049] Compared to the traditional method that requires moving the sampling needle 2 to a dedicated cleaning cup for cleaning, this solution eliminates the need for an additional cleaning station, enabling in-situ online cleaning, significantly shortening the cleaning process time and increasing detection throughput. Furthermore, this solution eliminates the need for complex switching mechanisms, simplifying the overall structure and improving system integration and reliability.

[0050] Preferably, the first moving mechanism 8 can precisely control the lifting speed and dwell time of the vibrating seat 4. Combined with the flow rate adjustment of the cleaning fluid, the cleaning parameters can be optimized for different reagent types to improve cleaning efficiency and energy saving.

[0051] In addition, the gap design between the sampling needle 2 and the second cavity 4102 not only reduces motion interference, but also helps the first elastic element 43 to clamp the first boss 5 again during the upward movement of the sampling needle 2 after the vibration seat 4 has finished cleaning.

[0052] According to some embodiments of the present invention, the first moving mechanism 8 includes: a first driving member 81, a guide rail 82, and a transmission assembly 83. The first driving member 81, the guide rail 82, and the transmission assembly 83 are all disposed on the frame 1. The transmission assembly 83 is connected to the vibration mechanism. The first driving member 81 is used to drive the transmission assembly 83 to move. The transmission assembly 83 drives the vibration mechanism to move up and down along the guide rail 82, so that the vibration seat 4 moves up and down.

[0053] like Figure 2 As shown, the transmission assembly 83 may include a transmission wheel 8301 and a transmission belt 8302. The first driving component 81, such as a motor, rotates to drive the transmission wheel 8301 and transmission belt 8302, thereby enabling the vibration mechanism to move up and down along the guide rail 82. The travel distance of the vibration mechanism can also be controlled by an optocoupler 10. Simultaneously, during the up-and-down movement of the vibrating seat 4, cleaning fluid is input and extracted through the liquid channel 44, thus cleaning the sampling needle. In other words, the vibrating seat 4 can function as a swab to clean the sampling needle.

[0054] According to some embodiments of this utility model, the vibrating element 3 includes: a second driving element 31 and a hollow cup swing element 32, wherein the hollow cup swing element 32 is connected to the output end of the second driving element 31. For example... Figure 2 As shown, the second driving component 31 is a motor, which is mounted on the frame 1. The hollow cup swivel 32 is installed at the output end of the motor and is connected to the sampling needle 2 to achieve vibration transmission. The hollow cup swivel 32 is equipped with an eccentric vibration block. When the motor runs, the hollow cup swivel 32 drives the eccentric vibration block to rotate together. Because the center of gravity of the eccentric vibration block is off-center from the rotation center, centrifugal force is generated, causing the motor to vibrate eccentrically. This, in turn, drives the sampling needle 2 to vibrate, thereby fully stirring the sample to be tested and improving the mixing effect.

[0055] According to some embodiments of the present invention, the frame 1 is provided with a second support 13, and the second support 13 extends laterally relative to the frame 1. The vibrating element 3 is connected to the second support 13, and the vibrating seat 4 is connected to the second support 13.

[0056] By providing a second, laterally extending bracket 13 on the frame 1 and connecting the vibrating element 3 and the vibrating seat 4 to the second bracket 13, a high-rigidity vibration mounting platform is formed, improving the overall stability of the vibration mechanism. Furthermore, the second bracket 13, as an independent support structure, can be located in a different area of ​​the frame 1 from the first bracket 11, avoiding mutual interference in their spatial layout.

[0057] In this embodiment, the first support 11 is disposed above and adjacent to the second support 13, so that the first elastic member 43 is flexibly connected to the distal end of the sampling needle 2 and the second elastic member 73 is flexibly impacted with the distal end of the sampling needle 2.

[0058] The sampling device S according to a second aspect embodiment of the present invention includes: a base plate 200, a second moving mechanism 300, and a liquid mixing device 100. The second moving mechanism 300 is disposed on the base plate 200 and connected to a test tube placement plate 400. The second moving mechanism 300 is used to drive the test tube placement plate 400 to move in the up-down, left-right, and front-back directions. The frame 1 is disposed on the base plate 200, and the test tube placement plate 400 can be moved to a position below the sampling needle 2 so that the sampling needle 2 can take samples from the test tubes on the test tube placement plate 400.

[0059] Specifically, the sample to be tested is placed in a test tube, and several test tubes are placed on a test tube rack. The test tube rack is placed on a test tube placement plate 400. The second moving mechanism 300 drives the test tube rack placement plate to move in the vertical, horizontal, and front-back directions, so that the test tube rack placement plate can move to below the sampling needle 2, and the sample to be tested in the test tube comes into contact with the sampling needle 2. Then, the sampling needle 2 is vibrated by a vibration mechanism. After the sample to be tested is fully mixed, the sampling needle 2 takes a sample. This can reduce the path length of the liquid path connecting the upper end of the sampling needle 2, and prevent the sampling needle 2 from moving continuously, causing the tubing to be repeatedly twisted or bent, which could cause cracks and lead to breakage or detachment from the upper end of the sampling needle 2.

[0060] In some embodiments, the second moving mechanism 300 includes x, y, and z-axis motion structures. The sample to be tested is mounted on the test tube rack placement plate via test tubes and a test tube rack. The z-axis motion structure enables the vertical movement of the sample to be tested. The z-axis motion structure is mounted and fixed on the y-axis motion structure via guide rail 82 to enable the forward and backward movement of the sample to be tested. The y-axis motion structure is fixed on the x-axis motion structure via guide rail 82 to enable the left and right movement of the sample to be tested. Thus, the sample to be tested can move in the x, y, and z directions, ensuring that the samples in the multiple test tubes arranged on the test tube rack can be controlled to move in the software so that they can be drawn and detected by the sampling needle 2.

[0061] Based on the above structure, the structure is first controlled in the software to the origin in the x, y, and z directions. Then, after the sample is loaded by the z-axis motion structure, the sampling needle 2 extends into the test tube containing the first sample to be tested. The second driving component 31 drives the hollow cup swing component 32 to rotate, vibrating the vibrating seat 4, which in turn drives the sampling needle 2 to rotate, stirring the sample. When the rotation stops, the sample is extracted. After extraction, the z-axis motion structure moves downward and controls the x-axis and y-axis motion structures to replace the sample to be tested. At this time, the vibrating seat 4 moves back and forth downward through the first moving mechanism 8, and the cleaning solution is input and extracted from the inside of the vibrating seat 4, completing the internal and external cleaning of the sampling needle 2. This cycle continues. That is, by controlling the sample loading, stirring, extraction, and cleaning of the sampling needle 2 through software, sample loading, stirring, extraction, and cleaning are all carried out in the same environment, reducing manual operation steps and achieving simple and fast detection.

[0062] A flow cytometer according to a third aspect of the present invention includes a sampler S.

[0063] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0064] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example.

[0065] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A liquid mixing device, characterized in that, include: Rack (1); Sampling needle (2), the sampling needle (2) is disposed on the frame (1); The vibration mechanism includes a vibrating element (3) and a vibrating seat (4). The vibrating element (3) is disposed on the frame (1) and connected to the vibrating seat (4). The vibrating element (3) is used to drive the vibrating seat (4) to vibrate. The vibrating seat (4) is provided with a first through cavity (41) through which the sampling needle (2) passes in the vertical direction. The side wall of the vibrating seat (4) is provided with at least one first transverse hole (42). One end of the first transverse hole (42) is connected to the first through cavity (41). The vibration seat (4) is configured to apply a radial clamping force to the sampling needle (2) in the first cavity (41) through a first elastic member (43) passing through the first transverse hole (42), so that the sampling needle (2) elastically wobbles in the first cavity (41) under the action of the vibration member (3).

2. The liquid mixing apparatus according to claim 1, characterized in that, There are multiple first transverse holes (42), and the multiple first transverse holes (42) are evenly distributed around the side wall of the vibration seat (4). Each first transverse hole (42) is provided with the first elastic element (43).

3. The liquid mixing apparatus according to claim 1 or 2, characterized in that, The sampling needle (2) is provided with a first protrusion (5); The first cavity (41) includes a first cavity segment (4101) and a second cavity segment (4102) connected in the vertical direction. The inner diameter of the first cavity segment (4101) is larger than the inner diameter of the second cavity segment (4102). The first transverse hole (42) is connected to the first cavity segment (4101). The inner diameter of the first boss (5) is adapted to the outer diameter of the first cavity segment (4101). A gap is provided between the sampling needle (2) and the second cavity segment (4102). The vibration seat (4) is configured such that the first elastic element (43) passes through the first transverse hole (42) to apply a radial clamping force to the first boss (5) in the first cavity segment (4101).

4. The liquid mixing apparatus according to claim 1, characterized in that, The sampling needle (2) is provided with a second protrusion (6), and the side wall of the second protrusion (6) is provided with a plurality of second transverse holes (61). The frame (1) is provided with a fixed seat (7), the fixed seat (7) is provided with a second through cavity (71) through which the sampling needle (2) passes in the vertical direction and a gap is provided between the second through cavity (71) and the sampling needle (2). The side wall of the fixed seat (7) is provided with a plurality of third transverse holes (72), one end of the third transverse hole (72) is connected to the second through cavity (71); the fixed seat (7) is configured to apply a radial abutment force to the inner wall of the second transverse hole (61) through a second elastic element (73) passing through the third transverse hole (72).

5. The liquid mixing apparatus according to claim 4, characterized in that, The first elastic member (43) and / or the second elastic member (73) include a fastening portion (91) and an elastic portion (92) connected to each other. The fastening portion (91) of the first elastic member (43) is fastened to the first transverse hole (42) and the elastic portion (92) applies a radial clamping force to the sampling needle (2). The fastening portion (91) of the second elastic member (73) is fastened to the third transverse hole (72) and the elastic portion (92) passes through the second transverse hole (61) and abuts against the inner wall of the hole.

6. The liquid mixing apparatus according to claim 4, characterized in that, The frame (1) is provided with a first bracket (11) and the first bracket (11) extends laterally relative to the frame (1), and the fixing seat (7) is fixed at the end of the first bracket (11) away from the frame (1).

7. The liquid mixing apparatus according to claim 1, characterized in that, Also includes: The first moving mechanism (8) is mounted on the frame (1) and is driven to the vibration mechanism. The first moving mechanism (8) is used to drive the vibration mechanism to move in the up and down direction. The vibration seat (4) is provided with a liquid passage (44) that communicates with the outside. The liquid passage (44) is connected to the first through cavity (41). The vibration seat (4) is configured to move up and down by the first moving mechanism (8). The cleaning fluid is input and extracted from the liquid passage (44).

8. The liquid mixing apparatus according to claim 7, characterized in that, The first moving mechanism (8) includes a first driving member (81), a guide rail (82), and a transmission assembly (83). The first driving member (81), the guide rail (82), and the transmission assembly (83) are all located on the frame (1). The transmission assembly (83) is connected to the vibration mechanism. The first driving member (81) is used to drive the transmission assembly (83) to move. The transmission assembly (83) drives the vibration mechanism to move up and down along the guide rail (82) so that the vibration seat (4) moves up and down.

9. The liquid mixing apparatus according to claim 1, characterized in that, The vibrating element (3) includes: a second driving element (31) and a hollow cup swing element (32), wherein the hollow cup swing element (32) is connected to the output end of the second driving element (31); and / or The frame (1) is provided with a second support (13) and the second support (13) extends laterally relative to the frame (1). The vibrating element (3) is connected to the second support (13), and the vibrating seat (4) is connected to the second support (13).

10. A sampling instrument, characterized in that, include: Base plate (200); The second moving mechanism (300) is disposed on the base plate (200) and connected to the test tube placement plate (400). The second moving mechanism (300) is used to drive the test tube placement plate (400) to move in the up-down direction, the left-right direction and the front-back direction. And the liquid mixing apparatus according to any one of claims 1-9, wherein the frame (1) is disposed on the base plate (200), and the test tube placement plate (400) is movable below the sampling needle (2) so that the sampling needle (2) can take samples from the test tubes on the test tube placement plate (400).

11. A flow cytometer, characterized in that, Includes the sampling device as described in claim 10.

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