Multiphase flow saturation-relative permeability relationship measuring device under different microorganism amounts

Abstract

The utility model discloses a kind of multiphase flow saturation-relatived permeability relationship measuring devices under different microbial amount, including seepage column, fluid component and measurement component, seepage column contains porous medium and multiple pore plate, multiple sampling holes and multiple pressure measuring holes are equipped in circumferential wall, top wall is equipped with water outlet, bottom wall is equipped with water inlet;Fluid component is connected with water inlet by peristaltic pump, including the first box that contains water phase, the second box that contains non-water phase and the third box that contains microorganism;Measurement component includes multiple pressure measuring tubes, multiple pressure sensors, first flowmeter, second flowmeter and colony counter, multiple pressure measuring tubes and multiple pressure measuring holes are one-to-one correspondence, pressure sensor is equipped on pressure measuring tube, first flowmeter is arranged between the first box and seepage column, second flowmeter is arranged between the second box and seepage column, and colony counter is arranged between the third box and seepage column;Two-phase flow and jamming-dredging dynamics experiment can be carried out, and saturation-relatived permeability-microbial amount constitutive relationship is analyzed.

Description

Technical Field

[0001] This utility model relates to the field of multiphase flow experimental technology in porous media, specifically to a device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial quantities. Background Technology

[0002] In the study of multiphase flow in porous media, the constitutive relationship between saturation (S) and relative permeability (kr) is a core kinetic parameter. Traditional studies typically consider only two- or three-phase flows of non-aqueous liquids (NAPL), water, and gas, neglecting the role of the microbial film phase. However, microbial growth and metabolism significantly alter the properties of porous media: on the one hand, the formation and bioclogging of microbial films change the pore structure, leading to repeated blockage and unblocking of pore spaces; on the other hand, metabolically produced gases and dissolved substances change the physicochemical properties of the fluid, thus affecting the measurements of saturation and relative permeability.

[0003] In existing technologies, experimental methods such as capillary pressure measurement and saturation measurement do not systematically incorporate microbial biomass parameters, which makes it impossible for traditional models to accurately describe the multiphase flow dynamics process under the influence of microorganisms. There is an urgent need to design targeted experimental devices to resolve the constitutive relationship of "saturation-relative permeability-microbial biomass (S-kr-br)". Utility Model Content

[0004] The main purpose of this invention is to provide a device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass, in order to solve the above-mentioned problems.

[0005] To achieve the above objectives, this utility model proposes a device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass, comprising: A seepage column extends vertically and contains a porous medium and multiple pore plates. The multiple pore plates are spaced apart along the vertical direction. The peripheral sidewall of the seepage column has multiple sampling holes and multiple pressure measuring holes, which are spaced apart along the vertical direction. The top wall of the seepage column has an outlet, and the bottom wall of the seepage column has an inlet. A fluid assembly, connected to the inlet via a first peristaltic pump, includes a first chamber, a second chamber, and a third chamber. The first chamber contains an aqueous phase, the second chamber contains a non-aqueous phase, and the third chamber contains microorganisms. The measurement assembly includes multiple pressure measuring tubes, multiple pressure sensors, a first flow meter, a second flow meter, and a colony counter. Each of the pressure measuring tubes corresponds to one of the multiple pressure measuring holes, and each pressure measuring tube is equipped with a pressure sensor for measuring the pressure difference along the fluid path to calculate the fluid seepage velocity. The first flow meter is located between the first housing and the seepage column and is used to measure the flow rate of the aqueous phase. The second flow meter is located between the second housing and the seepage column and is used to measure the flow rate of the non-aqueous phase. The colony counter is located between the third housing and the seepage column and is used to measure the number of microorganisms.

[0006] Optionally, the side pressure hole is provided with three, including an upper pressure measuring hole, a lower pressure measuring hole and a middle pressure measuring hole. The upper pressure measuring hole is located near the top wall of the seepage column, the lower pressure measuring hole is located near the bottom wall of the seepage column, and the middle pressure measuring hole is located at the middle of the seepage column. Correspondingly, there are three pressure measuring tubes.

[0007] Optionally, a second peristaltic pump is provided between the first box, the second box, and the third box and the seepage column, respectively.

[0008] Optionally, it also includes a heating element for heating the percolation column.

[0009] Optionally, it also includes a thermostat electrically connected to the heating element to control the temperature of the percolation column.

[0010] Optionally, the fluid assembly further includes a nutrient solution tank and an air compressor, wherein the nutrient solution tank contains nutrient solution; The third chamber is connected to the nutrient solution chamber and the air compressor, and a stirrer is installed inside the third chamber.

[0011] Optionally, the bottom wall of the seepage column is provided with two water inlets, including a first water inlet and a second water inlet. The first water inlet is connected to the first box and the second box through a first water inlet pipe, and the second water inlet is connected to the third box through a second water inlet pipe. An aeration head is provided at the second water inlet.

[0012] Optionally, it also includes a liquid collection tank, which is connected to the water outlet via a water outlet pipe.

[0013] Optionally, the porous medium includes porous ceramic particles and quartz sand, with the porous ceramic particles mixed into the quartz sand located in the middle of the seepage column.

[0014] In this invention, the device is capable of conducting two-phase flow experiments and clogging-clearing kinetic experiments. It fills a seepage column with porous media and combines a peristaltic pump for flow control, a pressure sensor to measure the pressure difference along the fluid flow path to calculate the fluid seepage velocity, and a flow meter and colony counter to measure the fluid flow rate for saturation calculation, relative permeability fitting, and microbial biomass quantification. This allows for the analysis of the constitutive relationship between saturation, relative permeability, and microbial biomass (S-kr-br). The measurement device provided by this invention can incorporate microbial biomass parameters into experiments, overcoming the limitations of traditional two-phase / three-phase flow models. It can accurately quantify the influence of the microbial membrane phase, integrating fluid mechanics, microbiology, and materials characterization techniques. This meets the high-precision experimental requirements of porous media-microorganism-multiphase flow coupled systems, providing experimental support and a theoretical model foundation for multiphase flow research in porous media in fields such as environmental remediation and oil extraction. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of an embodiment of the device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass provided by this utility model.

[0017] Explanation of icon numbers:

[0018] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] It should be noted that if the embodiments of this utility model involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0021] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0022] In the study of multiphase flow in porous media, the constitutive relationship between saturation (S) and relative permeability (kr) is a core kinetic parameter. Traditional studies typically consider only two- or three-phase flows of non-aqueous liquids (NAPL), water, and gas, neglecting the role of the microbial film phase. However, microbial growth and metabolism significantly alter the properties of porous media: on the one hand, the formation and bioclogging of microbial films change the pore structure, leading to repeated blockage and unblocking of pore spaces; on the other hand, metabolically produced gases and dissolved substances change the physicochemical properties of the fluid, thus affecting the measurements of saturation and relative permeability.

[0023] In existing technologies, experimental methods such as capillary pressure measurement and saturation measurement do not systematically incorporate microbial biomass parameters, which makes it impossible for traditional models to accurately describe the multiphase flow dynamics process under the influence of microorganisms. There is an urgent need to design targeted experimental devices to resolve the constitutive relationship of "saturation-relative permeability-microbial biomass (S-kr-br)".

[0024] In view of this, the present invention provides a device 100 for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass. Figure 1 An embodiment of the device 100 for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass provided by this utility model.

[0025] Please see Figure 1The device 100 for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass includes a seepage column 1, a fluid assembly 2, and a measuring component. The seepage column 1 extends vertically and contains a porous medium and multiple pore plates 4. The multiple pore plates 4 are spaced apart along the vertical direction. The peripheral wall of the seepage column 1 has multiple sampling holes 11 and multiple pressure measuring holes, which are spaced apart along the vertical direction. The top wall of the seepage column 1 has an outlet, and the bottom wall of the seepage column 1 has an inlet. The fluid assembly 2 is connected to the inlet via a first peristaltic pump and includes a first housing 21, a second housing 22, and a third housing 23. The first housing 21 is used to contain the aqueous phase, and the third housing 23... The second chamber 22 is used to contain the non-aqueous phase, and the third chamber 23 is used to contain microorganisms. The measuring assembly includes multiple pressure measuring tubes 3, multiple pressure sensors, a first flow meter, a second flow meter, and a colony counter. The multiple pressure measuring tubes 3 are arranged one-to-one with the multiple pressure measuring holes, and each pressure measuring tube 3 is equipped with a pressure sensor for measuring the pressure difference along the fluid to calculate the fluid seepage velocity. The first flow meter is located between the first chamber 21 and the seepage column 1 for measuring the flow rate of the aqueous phase. The second flow meter is located between the second chamber 22 and the seepage column 1 for measuring the flow rate of the non-aqueous phase. The colony counter is located between the third chamber 23 and the seepage column 1 for measuring the number of microorganisms.

[0026] In this invention, the device is capable of conducting two-phase flow experiments and clogging-clearing kinetic experiments. It fills the seepage column 1 with porous media and combines a peristaltic pump for flow control, a pressure sensor to measure the fluid pressure difference along the flow path to calculate the fluid seepage velocity, and a flow meter and colony counter to measure the fluid flow rate for saturation calculation, relative permeability fitting, and microbial biomass quantification. This allows for the analysis of the constitutive relationship between saturation, relative permeability, and microbial biomass (S-kr-br). The measurement device 100 provided by this invention can introduce microbial biomass parameters into experiments, overcoming the limitations of traditional two-phase / three-phase flow models. It can accurately quantify the influence of the microbial membrane phase, integrating fluid mechanics, microbiology, and materials characterization techniques. This meets the high-precision experimental requirements of porous media-microorganism-multiphase flow coupled systems, providing experimental support and a theoretical model foundation for multiphase flow research in porous media in fields such as environmental remediation and oil extraction.

[0027] It should be noted that, in this invention, the constitutive relationship of saturation-relative permeability-microbial biomass (S-kr-br) can be analyzed by fitting using the BCB model / VGM model.

[0028] It should also be noted that, in one embodiment of this utility model, the aqueous phase is deionized water and the non-aqueous phase (NAPL) is n-hexadecane.

[0029] Specifically, in one embodiment of this utility model, three pore plates 4 are provided, and the three pore plates 4 are distributed at intervals along the vertical direction, i.e., the axial direction of the seepage column 1, and the distance between any two adjacent pore plates 4 is 10 cm. The arrangement of the pore plates 4 can provide better attachment sites for the growth of microorganisms.

[0030] Specifically, in one embodiment of this utility model, the seepage column 1 is made of high-transmittance organic glass, with an inner diameter of 150mm±0.5mm and a column height of 390mm±1mm, to ensure the uniformity of radial flow of fluid.

[0031] More specifically, there are 7 sampling holes 11, which are distributed at intervals along the vertical direction, i.e., the axial direction of the seepage column 1, and are respectively 50mm, 100mm, 150mm, 200mm, 250mm, 300mm and 350mm away from the bottom reference surface of the seepage column 1. Each sampling hole is equipped with a sealing valve for multi-point dynamic fluid sampling.

[0032] For details, please refer to Figure 1 In one embodiment of this utility model, the side pressure hole is provided with three, including an upper pressure measuring hole, a lower pressure measuring hole and a middle pressure measuring hole. The upper pressure measuring hole is located near the top wall of the seepage column 1, the lower pressure measuring hole is located near the bottom wall of the seepage column 1, and the middle pressure measuring hole is located at the middle part of the seepage column 1; correspondingly, the pressure measuring tube 3 is provided with three.

[0033] More specifically, in one embodiment of this utility model, the upper pressure measuring hole is 20mm away from the top wall of the seepage column 1, and the lower pressure measuring hole is 20mm away from the bottom wall of the seepage column 1.

[0034] Specifically, in one embodiment of this utility model, the pressure measuring tube 3 is made of φ8mm hard glass tube.

[0035] Specifically, in one embodiment of this invention, the porous medium comprises porous ceramic particles and quartz sand, with the porous ceramic particles mixed into the quartz sand located in the middle of the seepage column 1. More specifically, when filling the quartz sand in the core growth zone of the biofilm (the 10-30cm area in the middle of the seepage column 1), 5% porous ceramic particles are mixed in, and the remainder is filled entirely with quartz sand. During the filling process of the porous medium, every 50mm of porous medium is filled, an axial pressure of 0.1MPa is applied and vibrated for 30s to ensure porosity uniformity. The porosity is calculated by the mass difference before and after saturation. In this way, the porosity uniformity error of the porous medium can be controlled within ±1.5%, providing a standardized medium substrate for microbial biofilm adhesion.

[0036] It should be noted that the quartz sand should be free of dust, have intact particles, and a pH value of 6.5-7.5 (tested with pH test paper or meter). The particle size of both the quartz sand and the porous ceramic particles should be 0.2-0.5 mm.

[0037] It should also be noted that the quartz sand used in the experiment within the seepage column 1 is treated quartz sand. Specifically, the quartz sand treatment process is as follows: the quartz sand is soaked in H2O2 solution for 24 hours, with 500g of quartz sand treated per liter of solution. Then, the quartz sand is soaked in 1% aminopropyltriethoxysilane solution (pH 8.0) for 2 hours. The soaked quartz sand is then transferred to a sieve (approximately 0.1 mm aperture) and slowly rinsed with water while gently shaking. Each rinse should cover the sand layer with water. After standing for 2-3 minutes, the water is drained. This rinsing process is repeated at least five times. The sand is then dried in a constant temperature oven at 105°C for 2-4 hours, or until a constant weight is achieved (i.e., the difference between two consecutive weighings is <0.1%). After drying, the sand is cooled to room temperature in a desiccator (approximately 30 minutes) and then sealed and stored in a dry container.

[0038] For details, please refer to Figure 1 In one embodiment of this utility model, a second peristaltic pump 5 is provided between the first box 21, the second box 22 and the third box 23 and the seepage column 1 respectively; in this way, fluid backflow can be prevented from disturbing the microbial community.

[0039] Specifically, in one embodiment of this utility model, a heating element is further included, which is used to heat the seepage column 1. More specifically, the heating element uses a water bath jacket heating method for heating, ensuring uniform heating and avoiding localized overheating. The heating element is a constant temperature water bath, etc.

[0040] Furthermore, it also includes a thermostat, which is electrically connected to the heating element to control the temperature of the percolation column 1 and maintain the temperature at 25±0.5℃.

[0041] For details, please refer to Figure 1 The fluid assembly 2 further includes a nutrient solution tank 24 and an air compressor 25. The nutrient solution tank 24 contains nutrient solution. A third tank 23 is connected to the nutrient solution tank 24 and the air compressor 25, and a stirrer 26 is installed inside the third tank 23. Thus, the nutrient solution in the nutrient solution tank 24 is cultured using the stirrer 26 and the air compressor 25 to obtain microorganisms.

[0042] More specifically, the nutrient solution is prepared in the ratio of glucose:NH4Cl:K2HPO4=10:1:1. The nutrient solution is introduced into the third chamber 23 for stirring and aeration to cultivate microorganisms. The dissolved oxygen is maintained at ≥4mg / L by turning on the air compressor 25, and the microorganisms are cultured for 4-7 days until they enter the stable growth period.

[0043] Specifically, the bottom wall of the seepage column 1 is provided with two water inlets, including a first water inlet and a second water inlet. The first water inlet is connected to the first box 21 and the second box 22 through a first water inlet pipe 6. The second water inlet is connected to the third box 23 through a second water inlet pipe 7. An aeration head is provided at the second water inlet to aerate and cultivate the nutrient solution, thereby regulating the nutrient supply and dissolved oxygen conditions required for microbial growth.

[0044] For details, please refer to Figure 1 It also includes a liquid collection tank 8, which is connected to the water outlet via a water outlet pipe to achieve gravity flow circulation of the fluid. More specifically, the water outlet pipe is a φ12mm pressure-resistant flexible hose.

[0045] The experimental steps of the multiphase flow saturation-relative permeability relationship measurement device under different microbial biomass provided by this utility model include: Step S1, Microbial Culture: Prepare the nutrient solution accurately according to the ratio of glucose:NH4Cl:K2HPO4 = 10:1:1. Place the nutrient solution into the third chamber, aerate it, and closely monitor the growth of the nutrient solution. Measure the OD of the nutrient solution daily. 600 Record the nutrient solution values, and environmental parameters such as incubation time, temperature, and pH. Before measurement, ensure the nutrient solution is uniformly suspended (by gently vortexing or shaking), avoiding sedimentation or air bubbles, and use the same culture medium or buffer as the nutrient solution as a blank control. Using a spectrophotometer, set the wavelength to 600 nm, first place the blank control sample, calibrate the instrument to OD value of 0, pour the nutrient solution sample into a clean cuvette, then place it in the spectrophotometer and read the OD value. 600 Value. Repeat the measurement 2-3 times and take the average value.

[0046] Step S2: Directly introduce a nutrient solution with a constant flow rate, adjusting the flow rate as needed. For example, in the seepage column with a diameter of 15cm, quartz sand (particle size 0.2-0.5mm) and porous ceramic particles (particle size 0.5-1mm) are mixed in a 1:1 ratio. Under this mixed material, the flow velocity of conventional groundwater is 0.115m / d, and correspondingly, 20ml of nutrient solution is introduced. Step S3, Aqueous phase saturation and initial parameter calibration: Aqueous phase (deionized water) is introduced into the first inlet of the permeation column until it is completely saturated. The inlet flow rate and outlet volume are recorded to calculate the initial saturation. Then, the aqueous phase is introduced into the peristaltic pump at a rate of 1 mL / min. The pressure difference of the three pressure measuring tubes is measured. The absolute permeability of the porous medium is calibrated using Darcy's law. Step S4, Two-phase flow experiment operation (start the experiment in 2-3 days): Initially, water saturation is used, and then a non-aqueous phase is introduced to drive the water out, thereby realizing the measurement under different non-aqueous phase / water saturation conditions. During the experiment, the flow rate of the peristaltic pump is precisely adjusted, and key parameters such as flow rate, pressure, and saturation of the two-phase flow are monitored in real time. The data are recorded in detail, and the relative permeability is calculated according to the basic equation of multiphase flow.

[0047] Step S5: Preliminary Data Processing: After the experiment, the collected multiphase flow parameter data are preliminarily processed, such as data filtering and outlier removal, to provide a reliable data foundation for subsequent data analysis. Step S6, Data Analysis: The experimental data were fitted using the BCB model, which represents the relative permeability under residual saturation. The parameter values ​​were determined through nonlinear regression, and the fitting error of the traditional model was compared to verify the effectiveness of the microbial correction term.

[0048] It should be noted that the device needs to be maintained and calibrated during long-term experiments. Every 10 days, the permeation column should be backwashed with deionized water at a flow rate of 5 mL / min for 2 hours to remove any EPS mucus that may be deposited, and the zero point of the pressure sensor should be recalibrated to ensure that the long-term measurement error is ≤±1%. When processing fluids containing particles, a 0.45 μm membrane filter should be installed upstream of the peristaltic pump.

[0049] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.

Claims

1. A device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass, characterized in that, The device for measuring the relationship between multiphase flow saturation and relative permeability under different microbial biomass includes: A seepage column extends vertically and contains a porous medium and multiple pore plates. The multiple pore plates are spaced apart along the vertical direction. The peripheral sidewall of the seepage column has multiple sampling holes and multiple pressure measuring holes, which are spaced apart along the vertical direction. The top wall of the seepage column has an outlet, and the bottom wall of the seepage column has an inlet. A fluid assembly, connected to the inlet via a first peristaltic pump, includes a first chamber, a second chamber, and a third chamber. The first chamber contains an aqueous phase, the second chamber contains a non-aqueous phase, and the third chamber contains microorganisms. The measurement assembly includes multiple pressure measuring tubes, multiple pressure sensors, a first flow meter, a second flow meter, and a colony counter. Each of the pressure measuring tubes corresponds to one of the multiple pressure measuring holes, and each pressure measuring tube is equipped with a pressure sensor for measuring the pressure difference along the fluid path to calculate the fluid seepage velocity. The first flow meter is located between the first housing and the seepage column and is used to measure the flow rate of the aqueous phase. The second flow meter is located between the second housing and the seepage column and is used to measure the flow rate of the non-aqueous phase. The colony counter is located between the third housing and the seepage column and is used to measure the number of microorganisms.

2. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, The pressure measuring hole is provided with three holes, including an upper pressure measuring hole, a lower pressure measuring hole and a middle pressure measuring hole. The upper pressure measuring hole is located near the top wall of the seepage column, the lower pressure measuring hole is located near the bottom wall of the seepage column, and the middle pressure measuring hole is located at the middle of the seepage column. Correspondingly, there are three pressure measuring tubes.

3. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, A second peristaltic pump is provided between the first box, the second box, and the third box and the seepage column, respectively.

4. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, It also includes a heating element for heating the percolation column.

5. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 4, characterized in that, It also includes a thermostat, which is electrically connected to the heating element to control the temperature of the percolation column.

6. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, The fluid assembly also includes a nutrient solution tank and an air compressor, wherein the nutrient solution tank contains nutrient solution. The third chamber is connected to the nutrient solution chamber and the air compressor, and a stirrer is installed inside the third chamber.

7. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, The bottom wall of the seepage column is provided with two water inlets, including a first water inlet and a second water inlet. The first water inlet is connected to the first box and the second box through a first water inlet pipe. The second water inlet is connected to the third box through a second water inlet pipe. An aeration head is provided at the second water inlet.

8. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, It also includes a liquid collection tank, which is connected to the water outlet via a water outlet pipe.

9. The device for measuring the relationship between saturation and relative permeability of multiphase flow under different microbial biomass as described in claim 1, characterized in that, The porous medium includes porous ceramic particles and quartz sand, with the porous ceramic particles mixed into the quartz sand located in the middle of the seepage column.

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