A device for batch and simple processing of morphological experimental material

Abstract

The application relates to a device for batch and simple processing of morphological experimental materials, which comprises an outer box group, an inner box and a cover net. The outer box group comprises one or more outer boxes, the outer boxes are filled with reagents for morphological experimental material reverse use, the upper part of the outer box is buckled with a box cover and forms a sealed space with the box cover, the box cover is provided with a cover handle at a position, the inner box is arranged in the outer box and the upper edge is vertically limited based on the box cover, a plurality of material grids for placing morphological experimental materials are arranged in the inner box, a plurality of reagent replacement holes are arranged at intervals between the side walls and the bottom of the material grid, a plurality of anti-falling buckles are arranged on the upper part of the inner box, and the cover net is arranged on the top of the inner box based on the anti-falling buckles. The device accurately meets the core needs of material form observation and diversified bearing of experiments, the integrated multi-grid layout is convenient for batch operation, a plurality of sample processing groups can be completed at a time, the operation efficiency and sample management convenience are greatly improved, material transfer damage is avoided, and the accuracy, reliability and operation safety of experimental results are comprehensively ensured.

Description

Technical Field

[0001] This application relates to the field of basic scientific research, and in particular to a device for batch and simple processing of morphological experimental materials. Background Technology

[0002] Sectioning techniques in morphological experiments are not only used to observe the morphological structure of normal cells and tissues in animals and plants, but are also a primary method used in pathology and forensic medicine to study, observe, and assess morphological changes in cells and tissues. Furthermore, they have been widely applied in research across many other disciplines. Paraffin sectioning, frozen sectioning, and vibrating sectioning techniques in morphological experiments are extremely common in morphological anatomy slide preparation. Ultrathin sectioning is also necessary when observing the ultrastructure of biological samples using transmission electron microscopy; and multiple treatments of biological materials using morphological experimental techniques are required when observing the ultrastructure of biological samples using scanning electron microscopy.

[0003] However, the preparation of morphological experimental materials involves a complex process, ranging from a few steps to dozens. Taking the processing of tissue materials into paraffin sections as an example, it requires steps such as fixation, washing, dehydration, pre-staining, elution, clearing, paraffin infiltration, and embedding to create a paraffin block containing the tissue material. Then, steps such as trimming the paraffin block, morphological experiments, slide preparation, and drying are followed to obtain tissue sections. The tissue section experiments involve steps such as dewaxing, elution, staining, elution, and mounting to obtain a complete, colored tissue morphological specimen. Many inorganic and organic solvents are used in the morphological processing of tissue materials. Different solvents require repeated treatments, and transitions between different solvents are necessary. Moreover, these solvent replacement steps are time-consuming, and manual solvent replacement operations are prone to errors or damage to the tissue material, not to mention the inherent risks involved.

[0004] Current centrifuge tube-based processing devices have the following drawbacks: Centrifuge tubes are independent tubular structures, each capable of holding only a small amount of material (such as small tissue pieces or sections). If multiple samples need to be processed simultaneously, frequent handling of different tubes is required. Furthermore, the long, thin tubes make it easy for material to adhere to the tube walls or accumulate, hindering rapid observation and handling. Each tube must be individually capped (to prevent leakage), making batch processing time-consuming and labor-intensive. Morphological experiments require extremely high material integrity (e.g., observing the structure of tissue sections and cell morphology), but the narrow tubular space of centrifuge tubes makes it easy for material (especially soft tissues and thin sections) to fold or be compressed during handling or shaking, leading to morphological deformation and affecting subsequent observation results. Morphological experiments often involve staining, rinsing, and dehydration steps, requiring sufficient contact between the material and reagents and easy solution replacement. The strong sealing of centrifuge tubes results in low reagent exchange efficiency, and the material tends to float inside, making stable immersion impossible and potentially leading to uneven processing (e.g., inconsistent staining depth). Utility Model Content

[0005] To address or partially address the problems existing in related technologies, this application provides a device for batch and convenient processing of morphological experimental materials, which is adapted to the core needs of experiments for material morphology observation and diversified support. The integrated multi-cell layout can process multiple groups of samples in batches.

[0006] The first aspect of this application provides an apparatus for batch and convenient processing of morphological experimental materials, comprising: The outer box assembly includes one or more outer boxes, which are filled with morphological experimental materials and reagents. The upper part of the outer box is fastened with a lid, which forms a sealed space with the lid. The lid has a handle. The inner box is placed inside the outer box, and the upper edge of the inner box is limited by the longitudinal direction of the lid. The inner box has multiple material compartments for placing morphological experimental materials. Multiple reagent replacement holes are spaced apart on the side walls and bottom of the material compartments. Multiple anti-detachment buckles are provided on the upper part of the inner box. The cover mesh is installed on the top of the inner box using anti-detachment clips.

[0007] The bottom of the outer box has a connecting part, and multiple outer boxes are stacked based on the connecting part to form a sealed environment.

[0008] The material grid has three side wall displacement holes on each of its four walls from top to bottom, and four bottom displacement holes on its bottom. The reagent liquid level is above the side wall displacement holes.

[0009] The inner box has four support points at the bottom, and the inner wall of the outer box has corresponding limit grooves for the support points.

[0010] Both the outer and inner boxes have frosted surfaces for writing or pasting labels.

[0011] The inner box has two handles at the top for removing it from the outer box.

[0012] The inner box has markings on the upper outer side, which are divided into horizontal and vertical markings.

[0013] The technical solution provided in this application may include the following beneficial effects: This application provides a device for batch and convenient processing of morphological experimental materials, which can accurately meet the core needs of experiments for material morphology observation and diverse support. The device adopts a visual design to support direct microscopic observation, and the tank structure can be customized according to different morphological materials such as sheet, block, and film to achieve stable positioning. At the same time, the integrated multi-compartment layout facilitates batch operation, completing the addition, incubation, and cleaning of multiple sets of samples at once. With uniform marking on the edge of the box, the operation efficiency and sample management convenience are greatly improved. The integrated design forms a convenient pipeline processing flow, eliminating the need to transfer materials one by one, which reduces operation steps and time costs, and avoids damage to materials during multiple transfers, thus comprehensively ensuring the accuracy, reliability, and operational safety of experimental results.

[0014] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0015] The above and other objects, features and advantages of this application will become more apparent from the more detailed description of exemplary embodiments thereof in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same components in the exemplary embodiments thereof.

[0016] Figure 1 This is a cross-sectional schematic diagram of the device shown in the embodiments of this application during material processing in the housing; Figure 2 This is a schematic cross-sectional view of the box of the device shown in the embodiments of this application when the material is floating; Figure 3 This is a schematic diagram of the assembly of the outer box and lid of the device shown in the embodiments of this application; Figure 4 This is a schematic diagram of the inner box of the device shown in the embodiments of this application; Figure 5 This is a schematic diagram of the material compartment of the inner box of the device shown in the embodiments of this application; Figure 6 This is a schematic diagram of the structure of the cover mesh of the device shown in the embodiments of this application; Reference numerals: In the figure, 1-outer box; 2-lid; 3-lid handle; 4-lid mesh; 5-reagent liquid level; 6-anti-detachment buckle; 7-inner box; 8-material compartment; 9-side wall replacement hole; 10-connecting part; 11-fulcrum; 12-bottom replacement hole; 13-morphological experimental material; 14-frosted part; 15-handle; 16-marked area. Detailed Implementation

[0017] Embodiments of this application will now be described in more detail with reference to the accompanying drawings. While embodiments of this application are shown in the drawings, it should be understood that this application may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make this application more thorough and complete, and to fully convey the scope of this application to those skilled in the art.

[0018] It should be understood that although the terms "first," "second," "third," etc., may be used in this application to describe various information, this information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, without departing from the scope of this application, first information may also be referred to as second information, and similarly, second information may also be referred to as first information. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0019] In the description of this application, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0020] Unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0021] The technical solutions of the embodiments of this application are described in detail below with reference to the accompanying drawings.

[0022] like Figure 1 The apparatus shown is for batch and simple processing of morphological experimental materials, comprising: an outer box assembly, a box cover 2, and an inner box 7.

[0023] The outer box group 1 includes one or more outer boxes 1. Inside the outer box 1 is an inner box 7 containing morphological experimental materials 13 and reagents used for processing the materials. A lid 2 is embedded in the top of the outer box 1. When the lid 2 is placed on top of the outer box 1, it exerts longitudinal pressure on the inner box 7 inside the outer box 1 to prevent the inner box 7 from shaking violently during processing. Figure 3 As shown, the lid 2 is made of transparent glass or transparent plastic. The state inside the outer box 1 can be observed through the lid 2. There is a handle 3 in the middle of the upper part of each lid 2, which can be used to lift and place the lid 2.

[0024] like Figure 4 As shown, the inner box 7 is an open-top box with an integrated structure of the four sides and internal material compartments 8, facilitating the placement of morphological experimental materials 13 into the material compartments 8. The material compartments 8 are configured with different sizes to accommodate morphological experimental materials 13 of varying shapes and dimensions. The four edges of the inner box 7 extend upwards, and four support points 11 are provided at the bottom. Anti-detachment buckles 6 are provided on the inner edges for attaching the cover net 4. In use, the inner box 7 is placed inside the outer box 1, and the morphological experimental materials 13 are placed in the material compartments 8 of the inner box 7. When the inner box 7 is placed inside the outer box 1, the four-point support 11 at the bottom provides stable support for the inner box 7 from below, limiting its lateral displacement. When the lid 2 closes the outer box 1, the lower surface of the lid 2 fits tightly against the upper surface of the extended edge of the inner box 7. On the one hand, the longitudinal pressure of the lid 2 "presses" the inner box 7 onto the bottom support 11, preventing the inner box 7 from moving up and down during vibrations. On the other hand, the inner side of the lid 2 can form a "tight gap fit" with the outer side of the extended edge of the inner box 7 (a groove or limiting edge is reserved on the inner side of the lid 2 to fit the extended edge), limiting the lateral swaying of the inner box 7 from all sides of the upper part. This is equivalent to "indirectly fixing" the inner box 7 in the center of the outer box 1 with the help of the lid 2. Ultimately, the inner box 7 is stably placed in the outer box 1, preventing shaking.

[0025] like Figure 6 The cover mesh 4 shown is tightly secured within the anti-detachment buckle 6. The cover mesh 4 has a relatively dense mesh and is slightly smaller than the inner wall of the inner box 7. It is positioned above the inner box 7 to prevent sample mixing caused by the morphological experimental material 13 in the material compartment 8 of the inner box 7 floating on the reagent surface. Figure 2 As shown, after the material floats to the top of the inner box 7, it is intercepted by the cover net 4 and finally confined in the material compartment 8.

[0026] The material grid 8 has multiple reagent replacement holes, including sidewall replacement holes 9 on the side walls and bottom replacement holes 12 at the bottom. The sidewall replacement holes 9 are distributed on all four walls of the material grid 8, arranged from top to bottom, with a total of three holes. The bottom replacement holes 12 are distributed at the bottom of the material grid 8, arranged symmetrically, with a total of four holes. Figure 5As shown. The bottom displacement hole 12 allows the treatment reagent contained in the outer box 1 to flow into and fill the material compartment 8, while the side wall displacement hole 9 facilitates the flow and displacement of the treatment reagent between the material compartments 8. When treating the material, the reagent liquid level 5 should be above the displacement hole of the inner box 7.

[0027] When using outer box 1 to hold treatment reagents, one or more outer boxes 1 can be selected to hold different treatment reagents according to different experimental requirements. Each outer box 1 is equipped with a lid 2, and inner boxes 7 with different diameter replacement holes are used according to the volume of the morphological experimental material 13. Outer box 1, lid 2, and inner box 7 are all made of transparent glass or transparent plastic, which allows operators to easily observe the experimental situation in real time and record the experimental results.

[0028] Both the outer box 1 and the inner box 7 have a frosted section 14 on the outside. Marks can be written on the frosted section 14 to distinguish the outer box 1 containing different reagents and the inner box 7 containing different batches of experimental materials. Even if multiple batches of experimental materials are processed at the same time, there will be no confusion.

[0029] The outer edge of the inner box 7 has markings 16, including horizontal and vertical markings. The experimental materials in each material compartment 8 of the inner box 7 correspond to the horizontal and vertical markings on the outer side. The corresponding material names can be recorded in a notebook or record table. There is no need to put paper or plastic labels in the material compartment 8, which avoids the influence of labels on materials and avoids the impact of damaged or incorrect labels on the experiment.

[0030] The inner box 7 can be used for treatment within the outer box 1 containing different treatment reagents. After the reagent treatment in one outer box 1 is completed, the operator only needs to lift the inner box 7 as a whole using the handle 15 on the inner box 7, and use the reagent replacement hole at the bottom to drain the reagent from the bottom of the material compartment 8 of the inner box 7. Then, it can be placed into another outer box 1 containing other treatment reagents for subsequent reagent replacement treatment. Using multiple outer boxes 1 and reagents can form an assembly line operation.

[0031] An apparatus consisting of an outer box 1 containing reagents, a lid 2, and an inner box 7 containing morphological experimental materials 13, or an apparatus consisting of one outer box 1 and one inner box 7 stacked with another apparatus consisting of one outer box 1 and one inner box 7, with the lid 2 placed on top, is placed on a horizontal shaker and slowly shaken to accelerate reagent replacement. During this process, the processing of the experimental materials can be observed through the transparent lid 2. Before the experiment, the lid 2 can be used to completely seal the outer box 1; or the connecting part 10 at the bottom of the outer box 1 can be inserted into the upper part of the lower outer box 1 to seal the lower outer box 1, with the lid 2 placed on top. This can be used for replacing reagents with irritating odors, ensuring the smooth progress of the experiment.

[0032] Finally, it should be noted that in this document, relationships such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "include," "contain," or any other variations are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.

[0033] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0034] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A device for batch and simple processing of morphological experimental materials, characterized in that, include: The outer box assembly includes one or more outer boxes (1), the outer box (1) is filled with morphological experimental materials (13) and reagents for reaction, the upper part of the outer box (1) is fastened with a box lid (2) and forms a sealed space with the box lid (2), and the box lid (2) has a lid handle (3). The inner box (7) is placed inside the outer box (1) and the upper edge of the inner box (7) is longitudinally limited by the lid (2). The inner box (7) has multiple material compartments (8) for placing morphological experimental materials (13). Multiple reagent replacement holes are spaced apart on the side wall and bottom of the material compartments (8). Multiple anti-detachment buckles (6) are provided on the upper part of the inner box (7). The cover net (4) is installed on top of the inner box (7) based on the anti-detachment buckle (6).

2. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, The material compartment (8) has three side wall replacement holes (9) on each of its four walls from top to bottom, and four bottom replacement holes (12) on its bottom. The reagent liquid level (5) for adding reagents is located above the side wall replacement holes (9).

3. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, The bottom of the inner box (7) has four support points (11), and the inner wall of the outer box (1) has a limit groove corresponding to the support points (11).

4. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, The inner box (7) has two handles (15) on the top for taking the inner box (7) out of the outer box (1).

5. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, The bottom of the outer box (1) is provided with a connecting part (10), and multiple outer boxes (1) are stacked based on the connecting part (10) to form a sealed environment.

6. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, Both the outer box (1) and the inner box (7) have frosted sections (14) on the outside for writing or pasting labels.

7. The apparatus for batch and convenient processing of morphological experimental materials according to claim 1, characterized in that, The upper part of the outer side of the inner box (7) is marked (16), and the marks are divided into horizontal marks and vertical marks.

Images