An immunoreaction device with controllable permeation rate

Abstract

The utility model discloses an immune reaction device of controllable percolation speed belongs to biological chip and diagnostic reagent technical field, and the reaction chamber and control room are connected, and the reaction chamber and control room are detachably connected, the reaction chamber includes the upper casing and the lower casing of the upper casing adaptation connection, the upper casing is fixedly connected with the lower casing reaction membrane between, the inside of lower casing is provided with waste liquid passage, and the outlet of waste liquid passage extends to the bottom of lower casing, the inside of control room is cavity structure, and the bottom of lower casing can insert the inside of control room, and the bottom of control room is provided with the detachable block valve, the immune reaction device of controllable percolation speed of the utility model, simple to make, convenient operation can be according to the needs of free, accurate control reaction process in the time of percolation.

Description

Technical Field

[0001] This utility model relates to the field of biochip and diagnostic reagent technology, and in particular to a controllable percolation immunoreaction device. Background Technology

[0002] Traditional immunoassay devices typically employ microfluidics to pressurize and accelerate the flow of the reaction solution when controlling the reaction rate. For example, Chinese Patent CN 120064646 A discloses an immunoassay microfluidic chip for the efficient enrichment and detection of Escherichia coli O157:H7 and its application. In this chip, the sample outlet is connected to an injection pump via a PEEK connector and a Teflon tube, while the sample inlet is connected to a silicone tube via a PEEK connector and a Teflon tube and inserted into the sample solution. In extraction mode, a negative pressure is created at the sample outlet using optimal sampling parameters, causing the sample solution to be drawn into the chip at the optimal temperature and with the set parameters. This is the design principle of traditional microfluidic chips.

[0003] However, this approach often results in problems such as more sophisticated and complex chip manufacturing equipment, higher costs, and greater operational difficulties due to the need to design even smaller microfluidic channels on tiny chips.

[0004] Furthermore, in the immunoassay process, the chips are often disposable. However, due to differences in sample concentration and composition, the free percolation rate varies, necessitating more intuitive and rapid adjustment of the percolation rate for each chip. Existing chips often rely on regulating pumps within the detection device to adjust the percolation rate, resulting in slow response to these adjustments and hindering precise control, thus affecting the stability and repeatability of the test results. Therefore, there is an urgent need for an immunoassay device that is simple in structure, low in cost, and capable of precisely controlling the percolation rate to improve the efficiency and accuracy of immunoassay detection. Utility Model Content

[0005] The technical problem to be solved by this invention is to design a novel immune reaction device with controllable percolation rate, which is simple to manufacture and easy to operate, and can freely and accurately control the percolation time during the reaction process as needed.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: an immunoassay device with controllable filtration rate, comprising a reaction chamber and a control chamber, wherein the reaction chamber and the control chamber are detachably connected; the reaction chamber comprises an upper shell and a lower shell adapted to and connected to the upper shell, wherein a reaction membrane is fixedly connected between the upper shell and the lower shell, wherein a waste liquid channel is provided inside the lower shell, and the outlet of the waste liquid channel extends to the bottom end of the lower shell; the control chamber has a hollow cavity structure inside, wherein the bottom end of the lower shell can be inserted into the interior of the control chamber, and a detachable blocking valve is provided at the bottom of the control chamber.

[0007] Furthermore, the cavity at the connection between the control chamber and the reaction chamber has a trapezoidal cross-section, and the lower part of the lower shell is inserted into the cavity of the control chamber, with its shape conforming to the cavity of the control chamber.

[0008] Furthermore, the lower housing is connected to the control chamber via a Luer joint.

[0009] Furthermore, the upper shell has a first conical cavity inside, and the narrowest part of the first conical cavity is located at the upper end of the center of the reaction membrane and is in contact with the reaction membrane.

[0010] Furthermore, a second conical cavity is provided inside the lower housing, the widest part of the second conical cavity being located at the lower end of the center of the reaction membrane and in contact with the lower surface of the reaction membrane.

[0011] Furthermore, the waste liquid channel includes a first waste liquid channel and a second waste liquid channel, the second waste liquid channel is fixedly connected to the first waste liquid channel and the diameter of the second waste liquid channel is larger than that of the first waste liquid channel, and the upper end of the first waste liquid channel is connected to the bottom of the second conical cavity.

[0012] Furthermore, a spacer ring is provided inside the upper housing, and a groove is provided at the contact point between the spacer ring and the inner wall of the upper housing. A corresponding protrusion is provided on the upper surface of the lower housing. After assembly, the protrusion fixes the reaction membrane to the upper housing through the spacer ring and presses the edge of the reaction membrane into the groove.

[0013] Furthermore, a hollow support ring is provided at the bottom of the protrusion of the lower shell, the support ring being used to support the water-absorbing pad.

[0014] Furthermore, both the upper and lower housings are made of ultrasonically weldable materials. When the upper and lower housings are fastened together, they are sealed and welded together by ultrasonic waves.

[0015] Furthermore, the blocking valve is a solenoid valve.

[0016] Beneficial effects: This application sets the control chamber as a cavity structure, and fills the cavity with water beforehand during the assembly process. Since the cavity is a sealed space at this time, the pressure in the cavity can be controlled by the blocking valve. That is, opening the blocking valve can control the water to flow out, thereby causing the reaction liquid to seep down. The speed at which the water flows out also determines the speed at which the reaction liquid seeps down.

[0017] Meanwhile, this application uses an ultrasonic fusion line for sealing when connecting the upper and lower housings, which can achieve a better sealing effect and is also the first application of ultrasonic fusion line in this field. Attached Figure Description

[0018] Figure 1This is a schematic diagram of the structure of the immune reaction device of this utility model.

[0019] Figure 2 This utility model Figure 1 A magnified view of A in the middle.

[0020] Figure 3 This is a schematic diagram of the structure of Embodiment 1 of this utility model.

[0021] Figure 4 This is a schematic diagram of the structure of the bottom of the upper shell in Embodiment 1 of this utility model.

[0022] Figure 5 This is a schematic diagram of the upper shell groove in Embodiment 1 of this utility model.

[0023] Figure 6 This is a schematic diagram of the lower shell structure of Embodiment 1 of this utility model.

[0024] Wherein, 1-reaction chamber, 11-upper shell, 111-groove, 112-first conical cavity, 113-partition ring, 12-lower shell, 121-protrusion, 122-support ring, 123-first waste liquid channel, 124-second waste liquid channel, 125-second conical cavity, 13-reaction membrane, 14-water-absorbing pad, 2-control chamber, 21-cavity, 3-blocking valve. Detailed Implementation

[0025] To enhance understanding of this utility model, it will be described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain this utility model and do not limit the scope of protection of this utility model.

[0026] like Figure 1 The diagram shows an immunoassay device with controllable filtration rate, comprising a reaction chamber 1 and a control chamber 2, which are detachably connected. Preferably, the reaction chamber 1 and the control chamber 2 can be connected by a Luer connector or by threads, but care must be taken to ensure their airtightness.

[0027] The reaction chamber 1 includes an upper shell 11 and a lower shell 12 adapted to and connected to the upper shell. A reaction membrane 13 is fixedly connected between the upper shell 11 and the lower shell 12. Preferably, a waterproof gasket 14 is fixedly provided between the upper shell 11 and the lower shell 12 at the lower part of the reaction membrane 13 to prevent leakage.

[0028] Preferably, both the upper and lower shells are made of materials that can be ultrasonically welded, thereby using ultrasonic welding to fix the upper and lower shells together; the reaction membrane is an NC membrane.

[0029] like Figure 1As shown, a first conical cavity 112 is provided inside the upper shell 11. The widest part of the first conical cavity 112 is located on the upper surface of the upper shell 11, and the narrowest part is located at the upper end of the center of the reaction membrane 13 and is in contact with the reaction membrane 13.

[0030] The lower housing 12 has a second conical cavity 125 inside. The widest part of the second conical cavity 125 is located at the lower end of the center of the reaction membrane 13 and is in contact with the lower surface of the reaction membrane 13.

[0031] The two conical cavities described above have two functions: the first conical cavity 112 facilitates the concentration of liquid on the reaction membrane, and the second conical cavity 125 facilitates the collection of the liquid after the reaction and allows it to flow downwards.

[0032] The lower housing 12 has a waste liquid channel inside. The inlet of the waste liquid channel is connected to the outlet of the second conical cavity 125, and the outlet of the waste liquid channel extends to the bottom of the lower housing 12. Specifically, the waste liquid channel includes a first waste liquid channel 123 and a second waste liquid channel 124, wherein the second waste liquid channel 124 is fixedly connected to the first waste liquid channel 123, preferably integrally formed, and the diameter of the second waste liquid channel 124 is larger than that of the first waste liquid channel 123. The upper end of the first waste liquid channel 123 is connected to the bottom of the second conical cavity.

[0033] The design that the diameter of the second waste liquid channel 124 is larger than that of the first waste liquid channel 123 makes the channel for the downward flow of waste liquid more spacious and smooth.

[0034] The control chamber 2 has a hollow internal structure, and the lower housing 12 is inserted into the cavity of the control chamber. A removable blocking valve 3 is installed at the bottom of the control chamber 2. To facilitate the adjustment of the water flow size and velocity, the blocking valve 3 can be a solenoid valve. Example 1

[0035] like Figure 3 The image shows a preferred embodiment of the present invention. In this embodiment, the assembled immune response device is generally cylindrical.

[0036] Specifically, such as Figures 4 to 6 As shown, the upper shell is cylindrical in shape with a funnel-shaped hollow interior. Looking upwards from the bottom, a baffle ring 113 extending from the inner wall towards the center can be seen; this is the bottom of the funnel-shaped cavity, which is also the bottom of the first conical cavity. The purpose of this baffle ring 113 is to cooperate with the protrusion 121 of the lower shell to fix the reaction membrane. A groove 111 is provided at the connection between the baffle ring 113 and the inner wall of the upper shell 11. After the operator assembles it by hand, the protrusion 121 of the lower shell 12 will extend into the interior of the upper shell 11, and the upper end of the protrusion 121 will press against the reaction membrane 13, pre-fixing it to the upper shell 11. A hollow support ring 122 is provided at the bottom of the protrusion 121 of the lower shell to support the absorbent pad 14.

[0037] This application uses ultrasonic welding to fix the upper and lower shells together. This design not only achieves a sealing effect on the upper and lower shells, but also allows the edge of the reaction membrane to be pressed into the groove 111 by the protrusion 121 under the pressure of ultrasonic waves, thereby further fixing the reaction membrane.

[0038] The cavity at the connection between control chamber 2 and reaction chamber 1 has a trapezoidal cross-section. Since the lower part of the lower housing 12 is inserted into the cavity of control chamber 2, the shape of the lower housing 12 must be adapted to the cavity of control chamber 2, and a waterproof seal is required. Preferably, the bottom end of the lower housing 12 can be inserted into the interior of control chamber 2 through a 6% Luer connector.

[0039] In the use of the immunoassay device of this application, the cavity is pre-filled with water. Since the cavity is a sealed space at this time, the pressure inside the cavity can be controlled by a blocking valve. That is, opening the blocking valve controls the outward flow of water, thereby causing the reaction solution to seep down. The speed at which the water flows outward also determines the speed at which the reaction solution seeps down. The use of a solenoid valve here allows for better control of the water flow rate and speed.

[0040] The embodiments disclosed herein are preferred embodiments, but are not limited thereto. Those skilled in the art can readily grasp the spirit of this utility model based on the above embodiments and make different extensions and variations. However, as long as they do not depart from the spirit of this utility model, they are all within the protection scope of this utility model.

Claims

1. An immunoassay device with controllable filtration rate, characterized in that, It includes a reaction chamber (1) and a control chamber (2), which are detachably connected; The reaction chamber (1) includes an upper shell (11) and a lower shell (12) adapted to and connected to the upper shell (11). A reaction membrane (13) is fixedly connected between the upper shell (11) and the lower shell (12). A waste liquid channel is provided inside the lower shell (12), and the outlet of the waste liquid channel extends to the bottom end of the lower shell (12). The interior of the control chamber (2) is a cavity (21), and the bottom end of the lower housing (12) can be inserted into the interior of the control chamber (2). A removable blocking valve (3) is provided at the bottom of the control chamber (2).

2. The immunoreaction device with controllable filtration rate according to claim 1, characterized in that, The cavity at the connection between the control chamber (2) and the reaction chamber (1) has a trapezoidal cross section. The lower part of the lower shell (12) is inserted into the cavity (21) of the control chamber (2), and its shape is adapted to the cavity (21) of the control chamber.

3. The immunoreaction device with controllable filtration rate according to claim 2, characterized in that, The lower housing (12) is connected to the control room (2) via a Luer joint.

4. The immunoreaction device with controllable filtration rate according to claim 1, characterized in that, The upper shell (11) is provided with a first conical cavity (112) inside. The narrowest part of the first conical cavity (112) is located at the upper end of the center of the reaction membrane (13) and is in contact with the reaction membrane (13).

5. The immunoreaction device with controllable percolation rate according to claim 1 or 4, characterized in that, The lower housing (12) is provided with a second conical cavity (125) inside. The widest part of the second conical cavity (125) is located at the lower end of the center of the reaction membrane (13) and is in contact with the lower surface of the reaction membrane (13).

6. The immunoreaction device with controllable filtration rate according to claim 5, characterized in that, The waste liquid channel includes a first waste liquid channel (123) and a second waste liquid channel (124). The second waste liquid channel (124) is fixedly connected to the first waste liquid channel (123) and the diameter of the second waste liquid channel (124) is larger than that of the first waste liquid channel (123). The upper end of the first waste liquid channel (123) is connected to the bottom of the second conical cavity (125).

7. The immunoreaction device with controllable filtration rate according to claim 1, characterized in that, The upper shell (11) is provided with a baffle ring (113) inside. The baffle ring (113) is provided with a groove (111) at the contact point with the inner wall of the upper shell (11). The upper surface of the lower shell (12) is provided with a corresponding protrusion (121). After assembly, the protrusion (121) fixes the reaction membrane (13) to the upper shell (11) through the baffle ring (113) and presses the edge of the reaction membrane (13) into the groove (111).

8. The immunoreaction device with controllable filtration rate according to claim 7, characterized in that, The bottom of the protrusion (121) of the lower shell is provided with a hollow support ring (122), which is used to support the water-absorbing pad.

9. The immunoreaction device with controllable filtration rate according to claim 1, characterized in that, The upper shell (11) and the lower shell (12) are both made of ultrasonically weldable materials. When the upper shell (11) and the lower shell (12) are fastened together, the upper shell (11) and the lower shell (12) are sealed and welded together by ultrasonic waves.

10. The immunoreaction device with controllable percolation rate according to claim 1, characterized in that, The blocking valve (3) is a solenoid valve.

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