Microscope device

Abstract

The present disclosure provides a microscope device, comprising: a microscope assembly, the microscope assembly being provided with an illumination interface; a light source assembly, the light source assembly comprising one output port, at least three light sources and a coupling mirror group arranged between the output port and the light sources, the emission bands of the three light sources being mutually exclusive, and the light paths of the three light sources being converged to the output port through the coupling mirror group; and a light path assembly, one end of the light path assembly being connected with the illumination interface of the microscope assembly, and the other end being in light path communication with the output port, for guiding the light beam of the output port into the back focal plane of the microscope objective in a coaxial manner to form an illumination field.

Description

Technical Field

[0001] This disclosure relates to a microscope apparatus, belonging to the field of microscopes. Background Technology

[0002] In numerous fields such as scientific research, medical diagnosis, and industrial inspection, the microscope plays an indispensable role as an important precision optical instrument. It helps people observe the fine structures and features of the microscopic world, providing crucial evidence for a deeper understanding of the nature of matter, the discovery of new phenomena, and precise quality control.

[0003] Conventional microscope sets have undergone long-term development in design and application, forming a relatively mature technical system. However, conventional microscope sets widely used in the market currently have a significant limitation: they are equipped with a single-source illumination path. While this single-source illumination path design can meet basic detection needs to a certain extent, its drawbacks are gradually becoming apparent with the continuous advancement of science and technology and the increasingly diverse and sophisticated detection requirements in various fields.

[0004] Different test samples have different physical and chemical properties, and therefore require different lighting conditions. For example, in the field of materials science, materials with different surface textures or internal structures require different light sources, which cannot be achieved by using a single light source.

[0005] In summary, conventional microscope sets, equipped with a single light source illumination path, result in limited detection conditions and cannot meet the increasingly diverse detection needs of different fields. Therefore, there is an urgent need to develop a microscope set with multiple light source illumination paths to overcome the shortcomings of existing technologies and provide support for detection work in various fields. Utility Model Content

[0006] This disclosure provides a microscope apparatus.

[0007] According to one aspect of this disclosure, a microscope apparatus is provided, comprising: a microscope assembly having an illumination interface; a light source assembly including an output port, at least three light sources, and a coupling mirror assembly disposed between the output port and the light sources, wherein the emission wavelengths of the three light sources do not overlap, and the light is focused to the output port by the coupling mirror assembly; and an optical path assembly, one end of which is connected to the illumination interface of the microscope assembly, and the other end of which is connected to the optical path of the output port, for guiding the light beam from the output port coaxially to the back focal plane of the microscope objective to form an illumination field.

[0008] According to one aspect of the technical solution of this disclosure, the microscope device integrates at least three light sources with non-overlapping emission bands and utilizes a coupling lens group to efficiently converge multiple beams to a single output port, achieving coaxial output of a multi-wavelength light field. Each light source independently emits a beam of a specific wavelength, which is then integrated by the coupling lens group and guided by an optical path assembly to the back focal plane of the microscope objective, forming an illumination field covering different spectral ranges. This solution effectively solves the limitation of conventional microscopes in the prior art, which are only equipped with a single light source and whose illumination path cannot adapt to diverse scenarios. By providing switchable or synchronous multi-wavelength illumination, this technology significantly expands the microscope's detection capabilities, allowing users to flexibly select illumination conditions according to sample characteristics (such as surface texture and chemical properties), thereby meeting different detection needs.

[0009] According to at least one embodiment of the microscope apparatus of this disclosure, the coupling lens group includes: a plano-convex aspherical lens having an incident plane and an exiting convex surface, the incident plane being disposed facing the light source; and a biconvex aspherical lens having a first optical surface and a second optical surface, the first optical surface being conjugate with the exiting convex surface, and the second optical surface being disposed facing the output port; wherein, light beams emitted from each light source enter the plano-convex aspherical lens through the incident plane and exit from the exiting convex surface to achieve collimation, and then are incident on the first optical surface of the biconvex aspherical lens and exit from the second optical surface to the output port to achieve focusing.

[0010] In this embodiment, the coupling lens group achieves beam collimation and focusing through a cascaded design of plano-convex aspherical lenses and biconvex aspherical lenses. The diverging beam emitted by the light source first enters through the incident plane of the plano-convex aspherical lens, where its aspherical structure performs preliminary collimation (reducing the divergence angle). The beam is then output from the exiting convex surface to the biconvex aspherical lens, where it is focused by the first optical surface and the exiting convex surface. Finally, the second optical surface converges the beam to the output port. The conjugate surface configuration of the aspherical lenses ensures high collimation of multiple beams during convergence, thereby improving optical path integration efficiency. Simultaneously, this structure simplifies traditional discrete optical path designs, avoiding uneven illumination fields caused by beam deflection, and laying the foundation for a stable illumination field.

[0011] According to at least one embodiment of the microscope apparatus of the present disclosure, the plano-convex aspherical lens is configured as an object-side optical interface, and the biconvex aspherical lens is configured as an image-side optical interface; wherein the object-side numerical aperture of the coupling lens group is 0.5, and the image-side numerical aperture is 0.3.

[0012] In this embodiment, the coupling lens group adopts a gradient aperture design with a high object-side NA (0.5) and a low image-side NA (0.3). A plano-convex aspherical lens serves as the object-side interface, collecting large-angle incident beams with a high numerical aperture of 0.5, adapting to the wide-angle emission characteristics of multiple light sources. A biconvex aspherical lens serves as the image-side interface, outputting the beam with a low numerical aperture of 0.3, ensuring the beam angle matches the total internal reflection conditions for fiber optic transmission. The beneficial effects of this design are: the high object-side NA significantly improves the beam collection efficiency of the light source, reducing light energy loss caused by angle mismatch; the low image-side NA ensures the output beam meets the critical angle requirements for fiber optic transmission, reducing beam scattering loss in the fiber, thereby improving the overall stability of light energy transmission and avoiding the illumination intensity attenuation problem caused by NA mismatch in traditional designs.

[0013] According to at least one embodiment of the microscope apparatus of the present disclosure, the light source assembly includes a base, each of the light sources is fixedly disposed on the base, and the base is provided with the output port.

[0014] In this embodiment, the base serves as a rigid support structure, precisely fixing multiple light sources in preset positions and forming an integrated connection with the output port. The rigid fixation of the base ensures the relative positional stability of the light sources and the coupling mirror assembly, avoiding optical path deviation caused by vibration or thermal deformation. At the same time, the direct integration of the output port with the base simplifies the assembly process and improves the environmental adaptability of the device.

[0015] According to at least one embodiment of the microscope apparatus of the present disclosure, the output port is provided with an optical fiber and is connected to the optical path assembly through the optical fiber.

[0016] In the technical solution of this embodiment, the beam output by the coupling mirror group is coupled to the optical fiber through the output port. The optical fiber, as a flexible medium, transmits the beam to the optical path components. The optical fiber connection provides spatial layout flexibility, allowing the light source components to be installed separately from the microscope, which facilitates system integration. At the same time, the waveguide characteristics of the optical fiber reduce stray light interference in the transmission path, ensuring the purity of the illumination light field and solving the installation and adaptation problem caused by space limitations in traditional rigid optical paths.

[0017] According to at least one embodiment of the microscope apparatus of the present disclosure, the optical path assembly includes: a carrier, the carrier having an optical fiber light source inlet connected to the optical fiber; and a lens assembly disposed on the carrier and configured to receive the light source introduced by the optical fiber and guide it into the microscope assembly for illumination.

[0018] In this embodiment, the optical fiber-transmitted light beam enters through the optical fiber light source inlet of the carrier, is received and shaped by the lens assembly, and then directed into the illumination interface of the microscope assembly. The carrier provides a precise mounting reference for the lens assembly, ensuring the optical axis alignment accuracy; the lens assembly then collimates and focuses the light beam output from the optical fiber, making it meet the incident requirements of the microscope illumination interface, thereby avoiding light energy waste and improving illumination efficiency, and solving the technical problem of multi-wavelength beams easily diverging at the interface.

[0019] According to at least one embodiment of the microscope apparatus of the present disclosure, the lens assembly includes: a first cemented lens, a second cemented lens, and a field lens; wherein, the light source introduced by the optical fiber passes sequentially through the first cemented lens, the second cemented lens, and the field lens, and the field lens images the light source onto the back focal plane of the microscope objective lens to form a Kohler illumination field.

[0020] In this embodiment, the lens assembly achieves Kohler illumination through a three-stage lens structure. The working principle is as follows: the light beam output from the optical fiber is initially collimated by a first cemented lens, and aberrations are corrected by a second cemented lens. Then, the field lens conjugates the light source (i.e., the output end of the optical fiber) onto the back focal plane of the microscope objective, forming a uniform illumination field. The introduction of the field lens ensures precise overlap between the image plane of the light source and the back focal plane of the objective, which is a core condition for Kohler illumination. The cemented lens group effectively suppresses chromatic aberration and spherical aberration, maintaining spectral consistency of multi-wavelength beams during imaging, ultimately forming a highly uniform illumination field. This significantly improves the vignetting problem commonly found in traditional illumination, providing an optical foundation for high-precision microscopic imaging.

[0021] According to at least one embodiment of the microscope apparatus of the present disclosure, the microscope assembly includes: a microscope imaging lens group; an image acquisition device, coaxially configured along the optical axis of the microscope imaging lens group, for real-time capture of optical images in the optical path of the microscope imaging lens group; and an objective lens unit, including an objective lens, which is coupled to the microscope imaging lens group through an optical interface to form a conjugate imaging optical path system.

[0022] In this embodiment, the microscope assembly integrates imaging and acquisition functions. The objective lens unit captures the light signals reflected or transmitted from the sample, which are then amplified by the microscopic imaging lens group and captured in real time by a coaxially configured image acquisition device. The illumination field introduced by the illumination device is strictly coaxial with the imaging optical path, ensuring the consistency of the optical axes of illumination and imaging. The conjugate imaging optical path system ensures that the illumination field uniformly covers the sample area, avoiding shadow interference caused by off-axis illumination. The coaxial configuration of the image acquisition device simplifies the optical path structure, reduces the light energy loss introduced by the beam splitter, thereby improving the image signal-to-noise ratio, which is especially suitable for the detection of transparent or semi-transparent samples requiring high-contrast imaging.

[0023] According to at least one embodiment of the microscope apparatus of the present disclosure, the objective lens unit further includes an objective lens converter, the number of objectives being at least two, the objective lens converter being used to switch objectives so that different objectives are coupled to the microscope imaging lens group.

[0024] In this embodiment, the objective turret rotates or translates mechanically to align multiple preset objectives sequentially with the optical axis of the microscope imaging array, achieving rapid coupling between the objectives and the imaging system. Users can switch between objectives of different magnifications (e.g., low-magnification objectives for wide-area scanning and high-magnification objectives for detailed observation) without interrupting the inspection process. Simultaneously, the coaxial light field of the illumination device automatically adapts to the back focal plane position of the new objective, ensuring that illumination uniformity is unaffected after switching. This eliminates the cumbersome operation of recalibrating the illumination when switching objectives in traditional microscopes.

[0025] The microscope apparatus according to at least one embodiment of the present disclosure further includes a control unit electrically connected to each of the light sources for independently controlling the switching state of each of the light sources to achieve the output of a single wavelength of light or the synchronous output of multiple wavelengths of light.

[0026] In this embodiment, the control unit enables flexible control of the light source. The working principle is as follows: the control unit receives user commands and independently controls the on / off state of each light source via electrical signals, thereby selectively activating a single light source (outputting a single wavelength of light) or multiple light sources (synchronously outputting multiple wavelengths of light). Users can quickly switch illumination modes according to sample characteristics without replacing hardware. Attached Figure Description

[0027] The accompanying drawings illustrate exemplary embodiments of the present disclosure and, together with the description thereof, serve to explain the principles of the present disclosure. These drawings are included to provide a further understanding of the present disclosure and are incorporated in and constitute a part of this specification.

[0028] Figure 1 This is a schematic diagram of a microscope apparatus according to one embodiment of the present disclosure.

[0029] Figure 2 This is a schematic diagram of the structure of a light source assembly according to one embodiment of the present disclosure.

[0030] Figure 3 This is a schematic diagram of the structure of a coupling mirror assembly according to one embodiment of the present disclosure.

[0031] Figure 4 This is a schematic diagram of the structure of an optical path assembly according to one embodiment of the present disclosure.

[0032] Figure 5 This is a schematic diagram of the structure of a lens assembly according to one embodiment of the present disclosure.

[0033] Figure 6 This is a schematic diagram of the structure of a microscope assembly according to one embodiment of the present disclosure.

[0034] The specific labels in the attached figures are as follows: 100 Microscope Components 110 Lighting Interface 120 Microscopic Imaging Lens Group 130 Image Acquisition Device 140 objective lens units 200 Light Source Components 210 Output Port 220 light source 230 Coupler Mirror Group 231 Plano-convex aspherical lens 231A Incident plane 231B Exit Convex 232 Biconvex aspherical lens 232A First Optical Surface 232B Second Optical Surface 240 base 250 fiber optic cable 300 optical path components 310 Bearing Base 311 Fiber Optic Light Source Entrance 320 Lens Assembly 321 First cemented lens 322 Second Cemented Lens 323 Scenes Detailed Implementation The present disclosure will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the disclosure. Furthermore, it should be noted that, for ease of description, only the parts relevant to the present disclosure are shown in the accompanying drawings.

[0035] It should be noted that, where there is no conflict, the embodiments and features described in this disclosure can be combined with each other. The technical solutions of this disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0036] Unless otherwise stated, the exemplary implementations / embodiments shown are to be understood as providing exemplary features of various details that provide ways in which the technical concepts of this disclosure can be implemented in practice. Therefore, unless otherwise stated, the features of various implementations / embodiments may be additionally combined, separated, interchanged and / or rearranged without departing from the technical concepts of this disclosure.

[0037] While conventional microscope arrays are well-designed and widely used in the market, most are equipped with a single light source illumination path. Although this can meet basic testing needs, its shortcomings become apparent with technological advancements and the increasing diversity and sophistication of testing requirements. Different samples require different illumination conditions, and a single light source cannot meet the diverse testing needs.

[0038] To address the aforementioned technical problems, this embodiment provides a microscope device.

[0039] Figure 1 This is a schematic diagram of a microscope apparatus according to one embodiment of the present disclosure.

[0040] See Figure 1 As shown, the microscope device provided in this embodiment includes: microscope assembly 100, light source assembly 200, and optical path assembly 300.

[0041] The microscope assembly 100 is equipped with an illumination interface 110.

[0042] Figure 2 This is a schematic diagram of the structure of a light source assembly according to one embodiment of the present disclosure.

[0043] like Figure 2 As shown, the light source assembly 200 includes an output port 210, at least three light sources 220, and a coupling mirror group 230 disposed between the output port 210 and the light sources 220. The emission bands of the three light sources 220 do not overlap, and the optical path is converged to the output port 210 through the coupling mirror group 230.

[0044] The optical path assembly 300 has one end connected to the illumination interface 110 of the microscope assembly 100 and the other end connected to the optical path of the output port 210. It is used to guide the light beam from the output port 210 into the back focal plane of the microscope objective in a coaxial manner to form an illumination field.

[0045] The microscope device described above, by configuring at least three non-overlapping wavelength light sources 220, combined with the converging function of the coupling lens group 230 and the coaxial insertion design of the optical path assembly 300, solves the problem of limited detection conditions in traditional single-light source microscopes. Through the synchronous or independent output of the multi-wavelength light sources 220, the illumination requirements of different samples for specific wavelengths can be met, achieving diversified adaptation of detection conditions.

[0046] Figure 3 This is a schematic diagram of the structure of a coupling mirror assembly according to one embodiment of the present disclosure.

[0047] like Figure 3As shown, in one embodiment of the coupling lens group 230, the coupling lens group 230 includes a plano-convex aspherical lens 231 and a biconvex aspherical lens 232. The plano-convex aspherical lens 231 is provided with an incident plane 231A and an exiting convex surface 231B, with the incident plane 231A facing the light source 220. The biconvex aspherical lens 232 is provided with a first optical surface 232A and a second optical surface 232B, with the first optical surface 232A and the exiting convex surface 231B being conjugate, and the second optical surface 232B facing the output port 210. The light beams emitted from each light source 220 enter the plano-convex aspherical lens 231 through the incident plane 231A, are collimated after exiting from the exiting convex surface 231B, and then enter the first optical surface 232A of the biconvex aspherical lens 232. After secondary collimation, they are output from the second optical surface 232B to the output port 210, achieving focusing. The plano-convex aspherical lens 231 and the biconvex aspherical lens 232 form a collimation and focusing structure. The incident plane 231A of the plano-convex lens receives the diverging beam for initial collimation, and the biconvex lens further completes the focusing, ensuring that the multi-band beam is focused at the output port 210.

[0048] Furthermore, the plano-convex aspherical lens 231 is configured as the object-side optical interface, and the biconvex aspherical lens 232 is configured as the image-side optical interface; wherein, the object-side numerical aperture of the coupling lens group is 0.5, and the image-side numerical aperture is 0.3. In the technical solution of this embodiment, through the matching design of numerical aperture (0.5 on the object side to 0.3 on the image side), the beam divergence angle is controlled while ensuring sufficient light throughput, so that the beam forms a small-angle light cone suitable for coaxial illumination of the microscope at the output port 210. This design optimizes the optical path transmission efficiency, avoids the aberration increase problem that may be caused by high numerical aperture, and ensures that the multi-band beam maintains high uniformity when it is introduced into the focal plane of the microscope objective.

[0049] like Figure 2 As shown, in one embodiment of the aforementioned light source assembly 200, the light source assembly 200 includes a base 240, with each light source 220 fixedly disposed on the base 240. The base 240 is provided with an output port 210. The structure of the base 240 realizes the integrated design of the light source 220 modules. By fixing multiple light sources 220 to the same base 240, the consistency of the output optical axis of each light source 220 is ensured, avoiding optical path offset caused by mechanical vibration or assembly errors. At the same time, the direct connection between the base 240 and the output port 210 shortens the optical path length and reduces light energy loss.

[0050] like Figure 1 and Figure 2As shown, the connection between the output port 210 and the optical path assembly 300 can be achieved by the following method: the output port 210 is equipped with an optical fiber 250, and is connected to the optical path assembly 300 through the optical fiber 250. The use of the optical fiber 250 enables a flexible connection between the light source assembly 200 and the optical path assembly 300. The light-guiding characteristics of the optical fiber 250 allow for flexible spatial arrangement of the optical path, adapting to the installation requirements of different microscope structures. At the same time, the anti-interference capability of the optical fiber 250 improves the system stability.

[0051] Figure 4 This is a schematic diagram of the structure of an optical path assembly according to one embodiment of the present disclosure.

[0052] like Figure 4 As shown, in some embodiments, the optical path assembly 300 includes a carrier 310 and a lens assembly 320. The carrier 310 is provided with an optical fiber light source inlet 311, which is connected to an optical fiber 250. The lens assembly 320 is disposed in the internal cavity of the carrier 310 and configured to receive the light source 220 introduced by the optical fiber 250 and guide it into the microscope assembly 100 for illumination. The carrier 310, as a structural carrier, ensures the precise alignment of the lens assembly 320 and the optical fiber 250 inlet. The lens assembly 320, through secondary optical design, shapes the light beam introduced by the optical fiber 250 into a beam shape suitable for coaxial illumination of the microscope, thereby improving the uniformity of light intensity in the illumination field.

[0053] Figure 5 This is a schematic diagram of the structure of a lens assembly according to one embodiment of the present disclosure.

[0054] like Figure 5 As shown, the lens assembly 320 further includes a first cemented lens 321, a second cemented lens 322, and a field lens 323. The light source 220, introduced by the optical fiber 250, passes sequentially through the first cemented lens 321, the second cemented lens 322, and the field lens 323. The field lens 323 images the light source 220 onto the rear focal plane of the microscope objective, forming a Kohler illumination field. The three-lens combination achieves uniform illumination field distribution through the Kohler illumination principle. The first cemented lens 321 and the second cemented lens 322 perform preliminary collimation and aberration correction of the beam, while the field lens 323 images the light source 220 onto the rear focal plane of the objective, ensuring a uniform field distribution of illumination light on the sample plane. This lens assembly 320 eliminates the bright spots or vignetting problems common in traditional illumination systems, improving the quality of microscopic imaging.

[0055] Figure 6 This is a schematic diagram of the structure of a microscope assembly according to one embodiment of the present disclosure.

[0056] like Figure 6As shown, in one embodiment of the microscope assembly 100 described above, the microscope assembly 100 includes: a microscope imaging lens group 120, an image acquisition device 130, and an objective lens unit 140. The microscope imaging lens group 120 can be a conventional microscope lens group. The image acquisition device 130 is coaxially configured along the optical axis of the microscope imaging lens group 120 and is used to capture optical images in the optical path of the microscope imaging lens group 120 in real time. For example, the image acquisition device 130 is a camera. The objective lens unit 140 includes an objective lens, which is coupled to the microscope imaging lens group 120 through an optical interface to form a conjugate imaging optical path system. In this embodiment, the coaxially configured image acquisition device 130 enables real-time monitoring of the microscope imaging optical path and supports dynamic adjustment of illumination parameters (such as light intensity and wavelength), while the conjugate imaging optical path system ensures high-fidelity transmission of sample images and avoids optical distortion through precise coupling between the objective lens and the imaging lens group.

[0057] To facilitate objective lens switching, the objective lens unit 140 also includes an objective lens converter. At least two objectives are included. The objective lens converter mechanically rotates or translates to sequentially align multiple preset objectives with the optical axis of the microscope imaging lens group 120, achieving rapid coupling between the objectives and the imaging system. The objective lens converter design supports rapid switching between objectives of different magnifications or numerical apertures to adapt to the needs of different inspection scenarios. Precise alignment of the objectives and imaging lens group is achieved through a mechanical structure, ensuring consistent image quality after switching and improving the system's inspection flexibility.

[0058] To achieve automated control, the microscope apparatus can also be equipped with a control unit. This control unit is electrically connected to each light source 220 and independently controls the on / off state of each light source 220 to achieve single-wavelength light output or synchronous output of multiple wavelengths. The control unit achieves dynamic adjustment of the light source 220 output through electronic control. This ability to independently control the on / off state of each light source 220 allows users to flexibly select single-band or multi-band illumination according to their detection needs. This supports both fine detection at specific wavelengths and composite detection requirements under multi-band synchronous illumination, significantly expanding the system's application range.

[0059] In the description of this specification, the references to terms such as "one embodiment / mode," "some embodiments / modes," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment / mode or example is included in at least one embodiment / mode or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment / mode or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments / modes or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments / modes or examples described in this specification, as well as the features of different embodiments / modes or examples.

[0060] Those skilled in the art should understand that the above embodiments are merely for illustrating the present disclosure and are not intended to limit the scope of the disclosure. Those skilled in the art can make other changes or modifications based on the above disclosure, and these changes or modifications still fall within the scope of the present disclosure.

Claims

1. A microscope apparatus, characterized in that, include: The microscope assembly is equipped with an illumination interface; A light source assembly includes an output port, at least three light sources, and a coupling mirror group disposed between the output port and the light sources. The emission wavelengths of the three light sources do not overlap, and the optical path is converged to the output port through the coupling mirror group. as well as An optical path assembly, one end of which is connected to the illumination interface of the microscope assembly, and the other end of which is connected to the output port optical path, is used to guide the light beam from the output port coaxially into the back focal plane of the microscope objective to form an illumination field.

2. The microscope apparatus according to claim 1, characterized in that, The coupling mirror assembly includes: A plano-convex aspherical lens, having an incident plane and an exit convex surface, wherein the incident plane is oriented towards the light source; and A biconvex aspherical lens is provided with a first optical surface and a second optical surface. The first optical surface is conjugate with the exiting convex surface, and the second optical surface is oriented toward the output port. In this process, the light beams emitted by each light source enter the plano-convex aspherical lens through the incident plane and are output from the exiting convex surface to achieve collimation. Subsequently, they are incident on the first optical surface of the biconvex aspherical lens and output from the second optical surface to the output port to achieve focusing.

3. The microscope apparatus according to claim 2, characterized in that, The plano-convex aspherical lens is configured as an object-side optical interface, and the biconvex aspherical lens is configured as an image-side optical interface. The object-side numerical aperture of the coupling mirror group is 0.5, and the image-side numerical aperture is 0.

3.

4. The microscope apparatus according to claim 1, characterized in that, The light source assembly includes a base, each of the light sources is fixedly disposed on the base, and the base is provided with the output port.

5. The microscope apparatus according to claim 1, characterized in that, The output port is equipped with an optical fiber and is connected to the optical path component through the optical fiber.

6. The microscope apparatus according to claim 5, characterized in that, The optical path component includes: A support base, wherein the support base is provided with an optical fiber light source inlet, and the optical fiber light source inlet is connected to the optical fiber; A lens assembly is disposed on the carrier and configured to receive the light source introduced by the optical fiber and guide it into the microscope assembly for illumination.

7. The microscope apparatus according to claim 6, characterized in that, The lens assembly includes: a first cemented lens, a second cemented lens, and a field lens; The light source introduced by the optical fiber passes sequentially through a first cemented lens, a second cemented lens, and a field lens. The field lens images the light source onto the back focal plane of the microscope objective, forming a Kohler illumination field.

8. The microscope apparatus according to claim 1, characterized in that, The microscope assembly includes: Microscopic imaging lens group; An image acquisition device is coaxially configured along the optical axis of the microscope imaging mirror group and is used to capture optical images in the optical path of the microscope imaging mirror group in real time. The objective lens unit includes an objective lens, which is coupled to the microscope imaging lens group through an optical interface to form a conjugate imaging optical path system.

9. The microscope apparatus according to claim 8, characterized in that, The objective lens unit also includes an objective lens converter, and the number of objectives is at least two. The objective lens converter is used to switch objectives so that different objectives are coupled to the microscope imaging lens group.

10. The microscope apparatus according to claim 1, characterized in that, It also includes a control unit, which is electrically connected to each of the light sources and is used to independently control the switching state of each of the light sources to achieve the output of a single wavelength of light or the synchronous output of multiple wavelengths of light.

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