Magnetic field focusing head, primary magnetic field device and biomolecule extraction apparatus

Abstract

The utility model discloses a kind of magnetic field focusing head, primary magnetic field device and biomolecule extraction equipment, magnetic field focusing head includes: permanent magnet, electromagnet and magnet conductor, electromagnet and permanent magnet are fixed on magnet conductor, one end of magnet conductor is formed into the magnetic field focusing end for towards secondary magnet to magnetize secondary magnet;Secondary magnet is the component that can be magnetized and demagnetized by magnetic field focusing head;Wherein, electromagnet is arranged as: by the direction of control current can make the magnetic pole of electromagnet and the magnetic pole of permanent magnet reverse or with the magnetic pole of permanent magnet same direction.In the scheme provided in the application, magnetic field focusing head can realize controllable magnetic field focusing, and the magnetic field intensity can be controlled by controlling current, so as to facilitate magnetization and demagnetization of secondary magnet.

Description

Technical Field

[0001] This utility model relates to the field of biomolecule extraction technology, and more specifically, to a magnetic field focusing head, a primary magnetic field device, and a biomolecule extraction equipment. Background Technology

[0002] One method for purifying or extracting biomolecules is to use magnetic nanobeads as carriers, separating biomolecules attached to the surface of the nanobeads from impurities in the original sample under the influence of a magnetic field. For example, the most common method used in magnetic nucleic acid purification technology is to use silica-coated magnetic beads, in which nucleic acids bind to the nanobeads in a high-salt solution. The nanobeads are collected by applying a magnetic field, thereby separating the nucleic acids from other cellular components or sample impurities. Finally, the purified nucleic acids are eluted with a low-salt buffer.

[0003] Currently, there are two specific methods for purifying or extracting biomolecules: 1. Use a magnet placed outside the container to attract the magnetic beads to the inner wall of the container. Then use a pipette to replace the liquid in the container and remove the contaminated solution. Repeat the above steps to clean the magnetic beads and elute nucleic acids from them.

[0004] 2. Use a magnetic rod placed inside the container and directly connected to the external machinery to attract the magnetic beads. Then move the magnetic rod to move the magnetic beads from one container to another, so that the magnetic beads are removed from the contaminated solution. Repeat the above steps to clean the magnetic beads and elute nucleic acids from them.

[0005] However, the current methods of biomolecule extraction have the following drawbacks: additional consumables are required to deliver magnetic beads (magnetic rod sleeves) or liquids (pipette tips). In addition, a robotic arm is required for moving the magnetic rod or handling the liquid. That is, peripheral devices (magnetic rods, pipette tips, etc.) need to be placed in the container, which results in bulky instrument structure and the risk of sample contamination. Utility Model Content

[0006] This utility model provides a magnetic field focusing head, a primary magnetic field device, and a biomolecule extraction device to address the deficiencies in existing biomolecule extraction methods.

[0007] An embodiment of this utility model provides a magnetic field focusing head, comprising: a permanent magnet, an electromagnet, and a magnetic conductor. The electromagnet and the permanent magnet are fixed on the magnetic conductor, and the electromagnet and the permanent magnet are configured with parallel magnetic pole directions. One end of the magnetic conductor is formed as a magnetic field focusing end for magnetizing a secondary magnet. The secondary magnet is a component that can be magnetized and demagnetized by the magnetic field focusing head. The electromagnet is configured such that, by controlling the direction of the current, the magnetic poles of the electromagnet are either opposite to or in the same direction as the magnetic poles of the permanent magnet.

[0008] In one embodiment, the magnetic conductor includes two parallel magnetic plates, the electromagnet and the permanent magnet are fixed between the two magnetic plates, and the magnetic pole directions of the electromagnet and the permanent magnet are both set from one magnetic plate to the other magnetic plate, with one end of the two magnetic plates forming the magnetic field focusing end.

[0009] In one embodiment, the magnetic field focusing end is configured to have a tapered cross-section along the direction toward the secondary magnet.

[0010] In one embodiment, at the magnetic field focusing end, the two side surfaces of each magnetic plate are inclined toward each other in the direction toward the secondary magnet so that the cross-section gradually narrows, and the two side surfaces of each magnetic plate are inclined toward each other in the direction toward the space between the two magnetic plates so that the end faces form trapezoidal end faces, and the small ends of the trapezoidal end faces of the two magnetic plates face each other.

[0011] In one embodiment, the secondary magnet is a cylindrical structure with a length of L and a diameter of D; wherein the length L of the secondary magnet is greater than the diameter D. The gap width A between the two magnetic plates is less than 3L; The minimum width C of each trapezoidal end face is less than 3D.

[0012] Embodiments of this application also provide a primary magnetic field device, including a mounting frame, a horizontally movable slider, a vertically movable slider, and a magnetic field focusing head as described above; wherein, The horizontally movable slider is horizontally movable relative to the mounting bracket, the vertically movable slider is movably mounted on the horizontally movable slider, and the magnetic field focusing head is mounted on the vertically movable slider.

[0013] In one embodiment, a rotation drive mechanism is provided between the magnetic field focusing head and the up-and-down moving slider, and the rotation drive mechanism is configured to drive the magnetic field focusing head to rotate.

[0014] In one embodiment, one or more magnetic field focusing heads are disposed on the up-and-down movable slider.

[0015] Embodiments of this application also provide a biomolecule extraction device, comprising: a container, magnetic beads, a secondary magnet, and a primary magnetic field device as described above; wherein, The container is provided with multiple receiving holes, and a connecting channel is provided between each pair of adjacent receiving holes; The magnetic beads are used to be placed in the receiving pore to adsorb biomolecules in the solution in the receiving pore; The magnetic field focusing head of the primary magnetic field device is movably disposed on the outside of the container; The secondary magnet is placed in the receiving hole. The magnetic field focusing head is configured such that when the magnetic poles of the electromagnet are in the same direction as the magnetic poles of the permanent magnet, the secondary magnet is magnetized to attract the secondary magnet; when the magnetic poles of the electromagnet are in opposite directions to the magnetic poles of the permanent magnet, the secondary magnet is demagnetized to move the secondary magnet away from the magnetic field focusing head. In this process, after the magnetic bead adsorbs biomolecules in the solution in one of the receiving holes, the secondary magnet in the receiving hole is magnetized by the magnetic field focusing head and can attract the magnetic bead. The magnetic field focusing head can move the secondary magnet with the attracted magnetic bead into another receiving hole by moving it. After the magnetic field focusing head demagnetizes the secondary magnet, the magnetic bead is dispersed in the solution in the receiving hole.

[0016] In one embodiment, the magnetic pole direction of the magnetic field focusing head is parallel to the hole wall it is attached to.

[0017] The magnetic field focusing head provided in this application can achieve controllable magnetic field focusing, and can control the strengthening and weakening of the magnetic field, thereby facilitating the magnetization and demagnetization of secondary magnets. For example, by controlling the direction of the current so that the magnetic poles of the electromagnet and the permanent magnet are opposite, the magnetic fields generated by the two cancel each other out, resulting in a weak or non-existent magnetic field generated by the focusing head. When the magnetic poles of the electromagnet and the permanent magnet are in the same direction, the magnetic fields generated by the two are superimposed, resulting in a stronger magnetic field generated by the focusing head. In addition, the magnetic field strength of the electromagnet can also be changed by controlling the current intensity of the electromagnet. For example, when there is no current in the electromagnet, the magnetic field generated by the focusing head is weakened.

[0018] The magnetic field focusing head provided in the embodiments of this application can be used for the extraction of biomolecules. It uses the magnetic field focusing head to drive the movement of the secondary magnet in the container to transfer the magnetic beads adsorbed with biomolecules. This can solve the problems of increased cost due to the need for additional consumables and easy sample contamination in the prior art, which uses a pipette to replace the liquid in the container or a magnetic rod to transport the magnetic beads.

[0019] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained by means of the structures particularly pointed out in the description and the drawings. Attached Figure Description

[0020] The accompanying drawings are provided to further illustrate the technical solution of this utility model and constitute a part of the specification. They are used together with the embodiments of this application to explain the technical solution of this utility model and do not constitute a limitation on the technical solution of this utility model.

[0021] Figure 1 This is a schematic diagram of the structure of a magnetic field focusing head according to one embodiment of this application; Figure 2 for Figure 1 The diagram shown is a view of the magnetic field focusing head from one side. Figure 3 for Figure 1 The diagram shown is a view of the magnetic field focusing head from one end. Figure 4 This is a schematic diagram of the structure of a secondary magnet according to one embodiment of this application; Figure 5 This is a schematic diagram illustrating the state in which the electromagnet and the permanent magnet in the magnetic field focusing head are aligned with each other, thereby attracting the secondary magnet, according to one embodiment of this application. Figure 6 This is a schematic diagram showing the state in which the electromagnet and the permanent magnet in the magnetic field focusing head are reversed, thus causing the secondary magnet to detach; according to one embodiment of this application. Figure 7 This is a schematic diagram of the structure of a primary magnetic field device according to one embodiment of this application; Figure 8 This is a schematic diagram of the structure of the primary magnetic field device and the container in one embodiment of this application; Figure 9 This is a schematic diagram of the primary magnetic field device according to another embodiment of this application; Figure 10 This is a schematic diagram of the structure of a primary magnetic field device according to another embodiment of this application; Figure 11 This is a schematic diagram of a biomolecule extraction device according to one embodiment of the present application, wherein magnetic beads are dispersed in a solution in a first receiving hole; Figure 12 for Figure 1 A schematic diagram of the biomolecule extraction device in the diagram, cut across the first receiving hole; Figure 13 This is a schematic diagram of the structure of a biomolecule extraction device according to one embodiment of the present application, wherein the magnetic beads in the first receiving hole are attracted by a secondary magnet; Figure 14 for Figure 13 A schematic diagram of the biomolecule extraction device in the diagram, cut across the first receiving hole; Figure 15This is a schematic diagram of a biomolecule extraction device according to one embodiment of the present application, wherein a secondary magnet is transferred into a second receiving hole and magnetic beads are dispersed in a solution; Figure 16 for Figure 15 A schematic diagram of the structure of the biomolecule extraction device cut at the second receiving hole.

[0022] Explanation of reference numerals in the attached figures: 1-Container; 11-Container body; 111-Container body; 112-Separation wall; 12-Container lid; 121-Lid plate body; 122-Baffle; 13-Accommodation hole; 13a-First accommodation hole; 13b-Second accommodation hole; 13c-Third accommodation hole; 14-Connecting channel; 2-Magnetic bead; 3-Primary magnetic field device; 31-Magnetic field focusing head; 311-Permanent magnet; 312-Electromagnet; 313-Magnetic conductor; 3131-Magnetic conductor plate; 3132-Magnetic field focusing end; 32-Motor; 33-Up-down moving slider; 34-Horizontal moving slider; 4-Secondary magnet. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be noted that, unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

[0024] Embodiments of this application provide a magnetic field focusing head 31, such as Figures 1-3 In the illustrated embodiment, the magnetic field focusing head 31 includes a permanent magnet 311, an electromagnet 312, and a magnetic conductor 313. The electromagnet 312 and the permanent magnet 311 are fixed on the magnetic conductor 313, and the electromagnet 312 and the permanent magnet 311 are configured with parallel magnetic pole directions, which refer to the direction from the S pole to the N pole or from the N pole to the S pole. One end of the magnetic conductor 313 is formed as a magnetic field focusing end 3132 for magnetizing the secondary magnet 4. The electromagnet 313 is configured such that, by controlling the direction of the current, the magnetic poles of the electromagnet 312 are either opposite to or in the same direction as the magnetic poles of the permanent magnet 311.

[0025] The magnetic conductor 313 can be made of a high-permeability material such as soft iron or silicon steel. The permeability of the magnetic conductor 313 is much higher than that of the surrounding air and other media. Magnetic lines of force will preferentially propagate along the path of the magnetic conductor 313, thereby changing the original propagation direction of the magnetic lines of force. Therefore, by setting the appropriate material, shape and size of the magnetic conductor 313, the magnetic flux density of the magnetic field focusing head 31 can be increased at the magnetic field focusing end 3132 to increase the magnetic field strength in its vicinity, i.e., focusing, thereby effectively magnetizing the secondary magnet 4.

[0026] The magnetic field focusing head 31 provided in the embodiments of this application can achieve controllable magnetic field focusing, and the magnetic field strength can be controlled, thereby facilitating the magnetization and demagnetization of the secondary magnet 4. For example, by controlling the direction of the current so that the magnetic poles of the electromagnet 312 are opposite to the magnetic poles of the permanent magnet 311, the magnetic fields generated by the two cancel each other out, resulting in a weak or non-existent magnetic field generated by the magnetic field focusing head 31. When the magnetic poles of the electromagnet 312 and the permanent magnet 311 are in the same direction, the magnetic fields generated by the two are superimposed, resulting in a stronger magnetic field generated by the magnetic field focusing head 31. In addition, the magnetic field strength of the electromagnet 312 can also be changed by controlling the current intensity of the electromagnet 312. For example, when there is no current in the electromagnet 312, the magnetic field generated by the magnetic field focusing head 31 is weakened.

[0027] The magnetic field focusing head 31 provided in this application can be used for the extraction of biomolecules. During the biomolecule extraction process, refer to... Figures 11-16 In one embodiment, magnetic beads 2 and a solution containing biomolecules are placed in one of the receiving holes 13 (e.g., the first receiving hole 13a) of container 1. Magnetic beads 2 are placed in the first receiving hole 13a to adsorb biomolecules in the solution within the first receiving hole 13a. A secondary magnet 4 is also provided in the first receiving hole 13a. Figure 11 and Figure 12 The displayed state. When the magnetic field focusing head 31 is positioned outside the container 1 and against the wall of the first receiving hole 13a, the magnetic field focusing head 31 can strengthen or weaken the magnetic field by controlling the current, thus magnetizing and demagnetizing the secondary magnet 4. The stronger the magnetic field of the magnetic field focusing head 31, the stronger the induced secondary magnetic field. When the magnetic field focusing head 31 has a strong magnetic field, the secondary magnet 4 is first attracted to the position closest to the magnetic field focusing head 31, at which point the secondary magnet 4 is further magnetized. The induced secondary magnetic field then attracts the magnetic beads 2 in the solution to both ends of the secondary magnet 4. Figures 13-14 As shown, when the magnetic field focusing head 31 moves, the secondary magnet 4 can follow suit. This movement of the magnetic field focusing head 31 carries the secondary magnet 4 into the second receiving hole 13b (there is a connecting channel between the first receiving hole 13a and the second receiving hole 13b). That is, the magnetic beads 2 adsorbed with biomolecules are transferred to the solution in the second receiving hole 13b for further washing. Then, the direction of the current in the electromagnet 312 is changed, causing the magnetic field of the magnetic field focusing head 31 to cancel each other out, demagnetizing the secondary magnet 4, and dispersing the magnetic beads 2 in the solution in the second receiving hole 13b. Then, in the same manner, the magnetic field focusing head 31 can be used to transfer the magnetic beads 2 adsorbed with biomolecules to the third receiving hole 13c for further elution, thereby achieving the extraction and purification of biomolecules.

[0028] Under the influence of a strong primary magnetic field, the speed at which the secondary magnet 4 approaches due to attraction from the magnetic field focusing head 31, or the speed at which the secondary magnet 4 detaches from the magnetic field focusing head 31 due to gravity in the absence of a primary magnetic field, is faster than the speed at which the secondary magnet 4 attracts the magnetic beads 2 to both ends of the secondary magnet 4. After the secondary magnet 4 is attracted to the focused strong magnetic field of the magnetic field focusing head 31, the induced secondary magnetic field will attract the magnetic beads 2 in the solution, thus achieving the purpose of attracting the magnetic beads 2 to both ends of the secondary magnet 4.

[0029] In the process of demagnetizing the secondary magnet 4 and dispersing the magnetic beads 2 in the solution, the magnetic field strength of the magnetic field focusing head 31 can be controlled to make the secondary magnet 4 rapidly and repeatedly attract and detach from the magnetic field focusing head 31, thereby creating turbulence in the liquid phase, which facilitates the mixing of liquid phase components and the dispersion of the magnetic beads 2.

[0030] Therefore, by using the magnetic field focusing head 31 provided in this application, the magnetic beads 2 can be conveniently concentrated, transferred, and dispersed during the extraction and purification of biomolecules, thereby improving the extraction efficiency of biomolecules. It is understood that the magnetic field focusing head 31 provided in this application can also be used in other applications requiring the magnetization and demagnetization of secondary magnets.

[0031] In one embodiment, such as Figures 1-3 As shown, the magnetic conductor 313 includes two parallel magnetic plates 3131. An electromagnet 312 and a permanent magnet 311 are fixed between the two magnetic plates 3131, and the magnetic pole directions of the electromagnet 312 and the permanent magnet 311 are both set from one magnetic plate 3131 toward the other magnetic plate 3131. The ends of the two magnetic plates 3131 facing the same direction form a magnetic field focusing end 3132. In this way, the magnetic field generated by the magnetic field focusing end 3132 is in the direction from one magnetic plate 3131 toward the other magnetic plate 3131. When the magnetic field focusing end 3132 abuts against the surface of the medium (e.g., the hole wall of container 1), the magnetic pole direction of the magnetic field focusing end 3132 can be parallel to the surface of the medium it abuts against.

[0032] like Figure 5 and Figure 6 As shown, the magnetic pole direction of the magnetic field focusing end 3132 is parallel to the surface of the medium it is in contact with. The magnetic pole direction of the secondary magnet 4, magnetized by the magnetic field focusing head 31, is also parallel to the surface of the medium. The magnetic beads 2 are focused at both ends of the secondary magnet 4. When the secondary magnet 4 adopts a cylindrical structure, the magnetic field focusing head 31 can drive the secondary magnet 4 to roll along the surface of the medium. In this way, the friction between the secondary magnet 4 and the attracted magnetic beads 2 and the surface of the medium is small, the loss of magnetic beads is small, and the movement efficiency is high. Figure 5 This demonstrates how the magnetic force of the magnetic field focusing head 31 strengthens the magnetization of the secondary magnet 4, causing the secondary magnet 4 to move towards the magnetic field focusing head 31 until it is attracted to the surface of the medium. Figure 6shows a state where the magnetic force of the magnetic field focusing head 31 is canceled, so that the secondary magnet 4 is demagnetized and moves away from the magnetic field focusing head 31 under the action of gravity.

[0033] In one embodiment, the magnetic field focusing end 3132 is configured to have a gradually tapered cross-section along a direction toward the secondary magnet 4 to be magnetized. Since for the same magnetic flux, a smaller cross-sectional area results in a greater magnetic flux density, by configuring the cross-section of the magnetic field focusing end 3132 to be gradually tapered, the magnetic flux density can be made greater at the magnetic field focusing end 3132, and the corresponding magnetic field is stronger.

[0034] as in Figure 2 and Figure 3 the example, both side surfaces of each magnetically conductive plate 3131 are inclined toward each other in the direction toward the secondary magnet 4 to be magnetized, so that the cross-section is gradually tapered, and both side surfaces of each magnetically conductive plate 3131 are inclined toward each other in the direction between the two magnetically conductive plates 3131, so that the end face forms a trapezoidal end face, and the small ends of the trapezoidal end faces of the two magnetically conductive plates 3131 face each other. That is, the area of the end face of the two magnetically conductive plates 3131 near the middle position is smaller, so that the magnetic force lines converge toward the middle position.

[0035] In one example, with reference to Figure 2 and Figure 3 , the gap width A between the two magnetically conductive plates 3131 is less than 3L, preferably, A=L; the minimum width C of the two trapezoidal end faces of the magnetic field focusing end 3132 is less than 3D. Wherein, L is the length of the cylindrical secondary magnet 4 to be magnetized, D is the diameter of the secondary magnet 4, D<L, with reference to Figure 4 the secondary magnet 4 shown in, wherein both ends of the secondary magnet 4 are flat surfaces or hemispherical surfaces.

[0036] the dimensions of the magnetically conductive body 313 are specifically set according to the dimensions of the secondary magnet 4, which enables the magnetic field focusing head 31 to be more suitable for magnetizing and transferring the secondary magnet 4.

[0037] It can be understood that the structure of the magnetic field focusing head 31 is not limited to the structure described above, and other modifications can be made. For example, Figures 1-3 the magnetic pole direction generated by the magnetic field focusing head 31 shown in is parallel to the surface of the medium to be abutted, and the structure of the magnetically conductive body 313 can also be arranged such that the magnetic pole direction is perpendicular to the surface of the medium to be abutted. Moreover, the permanent magnet 311 and the electromagnet 312 are not limited to being arranged side by side, for example, the electromagnet 312 can also be arranged concentrically with the permanent magnet 311, such as arranged around the permanent magnet 311.

[0038] Embodiments of the present application further provide a primary magnetic field device 3, as in Figures 7-10As shown, it includes a mounting bracket, a horizontally movable slider 34, a vertically movable slider 33, and a magnetic field focusing head 31 as described above. The horizontally movable slider 34 is horizontally movable relative to the mounting bracket, the vertically movable slider 33 is movably mounted on the horizontally movable slider 34, and the magnetic field focusing head 31 is mounted on the vertically movable slider 33.

[0039] The primary magnetic field device 3 provided in this application can drive the magnetic field focusing head 31 to move up and down and horizontally, thereby enabling the movement of the magnetic field focusing head 31 through the primary magnetic field device 3, thus automating the movement of the secondary magnet 4 during the biomolecule extraction process.

[0040] In one embodiment, a rotation drive mechanism is provided between the magnetic field focusing head 31 and the vertically moving slider 33. The rotation drive mechanism is configured to drive the magnetic field focusing head 31 to rotate. This rotation drive mechanism can be a motor 32, which drives the magnetic field focusing head 31 to rotate. The rotation of the magnetic field focusing head 31 drives the secondary magnet 4 to rotate in a certain direction, making it more suitable for moving along a predetermined direction.

[0041] In one embodiment, one or more magnetic field focusing heads 31 may be provided on the up-and-down moving slider 33, and each magnetic field focusing head 31 may be provided with a rotating mechanism between it and the up-and-down moving slider 33.

[0042] like Figure 7 and Figure 8 The primary magnetic field device 3 has a magnetic field focusing head 31 mounted on its vertically movable slider 33. Figure 8 The magnetic field focusing head 31 is shown resting against the outer wall of the container 1, which has multiple receiving holes.

[0043] Figure 9 and Figure 10 The image shows that four magnetic field focusing heads 31 are set on the up-and-down sliding slider 33, among which... Figure 9 The image shows that four motors 32 are arranged in an array on the up-and-down moving slider 33, and each motor 32 is connected to a magnetic field focusing head 31. Figure 10 The diagram shows that two motors 32 are respectively installed on the vertically moving slider 33, and each motor 32 is connected to a magnetic field focusing head 31 at both ends. Of course, other numbers of magnetic field focusing heads 31 can also be installed on the vertically moving slider 33. Each primary magnetic field device 3 is equipped with multiple magnetic field focusing heads 31, which can simultaneously perform biomolecule extraction steps from multiple samples.

[0044] Embodiments of this application also provide a biomolecule extraction device, such as... Figures 11-16 As shown, it includes: a container 1, a magnetic bead 2, a secondary magnet 4, and a primary magnetic field device 3 as described above; wherein, Container 1 is provided with multiple receiving holes 13, and a connecting channel 14 is provided between each pair of adjacent receiving holes 13; magnetic beads 2 are used to be placed in the receiving holes 13 to adsorb biomolecules in the solution in the receiving holes 13; the magnetic field focusing head 31 of the primary magnetic field device 3 is movably set on the outside of container 1; secondary magnet 4 is used to be placed in the receiving holes 13, and the magnetic field focusing head 31 is configured to magnetize the secondary magnet 4 when the magnetic poles of the electromagnet 312 are in the same direction as the magnetic poles of the permanent magnet 311, and demagnetize the secondary magnet 4 when the magnetic poles of the electromagnet 312 are in opposite directions to the magnetic poles of the permanent magnet 311.

[0045] In this process, after the magnetic bead 2 adsorbs biomolecules in the solution in one of the receiving holes 13, the secondary magnet 4 located in the receiving hole 13 is magnetized by the magnetic field focusing head 31 and can attract the magnetic bead 2. The magnetic field focusing head 31 can move the secondary magnet 4 with the magnetic bead 2 attracted into another receiving hole 13 by moving. After the magnetic field focusing head 31 demagnetizes the secondary magnet 4, the magnetic bead 2 is dispersed in the solution in the receiving hole 13.

[0046] Figures 11-16 The container 1 is shown to have multiple receiving holes 13, including a first receiving hole 13a, a second receiving hole 13b, a third receiving hole 13c, etc.

[0047] Figure 11 and Figure 12 The diagram shows a first receiving hole 13a containing magnetic beads 2 and a solution containing biomolecules. Within this first receiving hole 13a, the magnetic beads 2 and the biomolecules combine to form a complex. The magnetic beads 2 can be superparamagnetic beads. When an external magnetic field is present, the superparamagnetic beads are magnetized and attracted to both ends of the secondary magnet 4. When no external magnetic field is present, the superparamagnetic beads are easily demagnetized due to thermodynamic effects, exhibiting no hysteresis and not agglomerating; they can disperse in the solution. Furthermore, superparamagnetic beads are more easily magnetized and have a stronger attraction than paramagnetic beads.

[0048] In this application, biomolecular binding refers to the non-covalent binding of a biomolecule with another object, such as adsorption, including physical adsorption, chemical adsorption, affinity adsorption, etc.

[0049] Figure 13 and Figure 14 The diagram shows the magnetic field focusing head 31 magnetizing the secondary magnet 4, with the secondary magnet 4 attracting the magnetic bead 2 in the first receiving hole 13a. As the magnetic field focusing head 31 moves upward, the secondary magnet 4 follows and moves upward. After moving upward and passing through the connecting channel 14 between the first receiving hole 13a and the second receiving hole 13b, the secondary magnet 4 enters the second receiving hole 13b.

[0050] Figure 15 and Figure 16The diagram shows the state of the secondary magnet 4 after it enters the second receiving hole 13b, after which the secondary magnet 4 is demagnetized and the magnetic beads 2 are dispersed in the solution.

[0051] The biomolecule extraction device provided in the embodiments of this application uses a magnetic field focusing head 31 to drive the secondary magnet 4 in the container to move, thereby transferring the magnetic beads 2 adsorbed with biomolecules. This can solve the problems of increased cost due to the need for additional consumables and easy sample contamination in the prior art, which uses a pipette to replace the liquid in the container or a magnetic rod to transport the magnetic beads.

[0052] In one embodiment, the magnetic pole direction of the magnetic field focusing head 31 is parallel to the wall of the hole it is attached to, and the secondary magnet 4 is a cylindrical magnet. In this way, when the magnetic field focusing head 31 applies a magnetic field, the secondary magnet 4 is magnetized, and the magnetic beads 2 are attracted to both ends of the secondary magnet 4. The axis of the secondary magnet 4, i.e., the magnetic field pole direction, is parallel to the wall surface. During the process of the magnetic field focusing head 31 moving the secondary magnet 4, the secondary magnet 4 can roll along the wall surface. The magnetic beads 2 attracted to both ends of the secondary magnet 4 do not rub against the wall surface, so the loss of magnetic beads is small.

[0053] In one embodiment, the container 1 includes a container body 11 and a container lid 12 covering the container body 11. The container body 11 is provided with a plurality of receiving holes 13, and the container lid 12 is configured to cover the plurality of receiving holes 13. By providing the container lid 12, the problem of the prior art where the container is not sealed, the magnetic beads 2 are exposed, and aerosols are easily generated or come into contact with other samples can be solved.

[0054] In one example, reference Figures 11-16 The container body 11 includes an outer wall 111 and a partition wall 112 disposed between two adjacent receiving holes 13. The container lid 12 is configured to include a cover plate body 121 covering the top of the receiving hole 13 and baffles 122 disposed on the inner side of the cover plate body 121 and respectively corresponding to the partition walls 112. The container lid 12 is configured to have a first position and a second position when it is placed on the container body 11, with the first position below the second position. In the second position, a gap is formed between the baffle 122 and the corresponding partition wall 112, forming a communication channel 14 between the two receiving holes 13. In the first position, the baffle 122 moves down to the partition wall 112 and closes the communication channel 14.

[0055] Each well 13 contains a different reagent that cannot be mixed. During transport, the connecting channel 14 between the wells 13 must be closed. During biomolecule extraction, the magnetic beads 2 carry biomolecules from one well to another for different purposes, such as binding to biomolecules, removing impurities, or releasing biomolecules. Closing the connecting channel 14 prevents the mixing of reagents in different wells, so as not to affect the purity of the final biomolecules.

[0056] In the description of this utility model, biomolecules may include nucleic acid molecules, such as ribonucleic acid (RNA) and deoxyribonucleic acid (DNA), protein molecules, such as antibody molecules, antigen molecules, biological enzymes, receptors, growth factors, and other organic molecules that originate from or are related to or can act on organisms.

[0057] In this invention, the terms attraction, adsorption, and binding are interchangeable, and their specific meanings can be determined according to the context. Generally speaking, attraction can be an interaction that occurs before or after physical contact between two objects, while adsorption and binding are effects or functions that occur after physical contact. Adsorption of biomolecules can include physical adsorption, chemical adsorption, affinity adsorption, etc.

[0058] In the description of this utility model, it should be noted that the terms "upper", "lower", "one side", "the other side", "one end", "the other end", "side", "opposite", "four corners", "periphery", "'mouth' structure", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the structure referred to has a specific orientation, or is constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0059] Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific location, or specific order of the indicated technical features. Therefore, a feature defined with "first," "second," etc., may explicitly or implicitly include at least one of those features.

[0060] In the description of the embodiments of this utility model, unless otherwise expressly specified and limited, the terms "connection," "direct connection," "indirect connection," "fixed connection," "installation," and "assembly" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection. The terms "installation," "connection," and "fixed connection" can refer to a direct connection or an indirect connection through an intermediate medium, or they can refer to the internal communication between two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0061] Although the embodiments disclosed in this utility model are as described above, the content described is only for the purpose of facilitating understanding of this utility model and is not intended to limit this utility model. Any person skilled in the art to which this utility model pertains may make any modifications and changes in the form and details of the implementation without departing from the spirit and scope disclosed in this utility model, but the patent protection scope of this utility model shall still be defined by the appended claims.

Claims

1. A magnetic field focusing head, characterized in that, include: A permanent magnet, an electromagnet, and a magnetic conductor, wherein the electromagnet and the permanent magnet are fixed on the magnetic conductor, and the electromagnet and the permanent magnet are configured to have parallel magnetic pole directions; One end of the magnetic conductor is formed as a magnetic field focusing end for magnetizing the secondary magnet; the secondary magnet is a component that can be magnetized and demagnetized by the magnetic field focusing head. The electromagnet is configured such that, by controlling the direction of the current, the magnetic poles of the electromagnet are either opposite to or in the same direction as the magnetic poles of the permanent magnet.

2. The magnetic field focusing head according to claim 1, characterized in that, The magnetic conductor includes two parallel magnetic plates. The electromagnet and the permanent magnet are fixed between the two magnetic plates, and the magnetic poles of the electromagnet and the permanent magnet are both set to face from one magnetic plate to the other magnetic plate. One end of the two magnetic plates forms the magnetic field focusing end.

3. The magnetic field focusing head according to claim 2, characterized in that, The magnetic field focusing end is configured to have a tapered cross-section along the direction toward the secondary magnet.

4. The magnetic field focusing head according to claim 3, characterized in that, At the magnetic field focusing end, the two sides of each magnetic plate are inclined toward each other in the direction toward the secondary magnet so that the cross-section gradually narrows, and the two sides of each magnetic plate are inclined toward each other in the direction toward the space between the two magnetic plates so that the end faces form trapezoidal end faces, and the small ends of the trapezoidal end faces of the two magnetic plates face each other.

5. The magnetic field focusing head according to claim 4, characterized in that, The secondary magnet is a cylindrical structure with a length of L and a diameter of D; wherein the length L of the secondary magnet is greater than the diameter D. The gap width A between the two magnetic plates is less than 3L; The minimum width C of each trapezoidal end face is less than 3D.

6. A primary magnetic field device, characterized in that, It includes a mounting bracket, a horizontally moving slider, a vertically moving slider, and a magnetic field focusing head according to any one of claims 1-5; wherein, The horizontally movable slider is horizontally movable relative to the mounting bracket, the vertically movable slider is movably mounted on the horizontally movable slider, and the magnetic field focusing head is mounted on the vertically movable slider.

7. The primary magnetic field device according to claim 6, characterized in that, A rotation drive mechanism is provided between the magnetic field focusing head and the up-and-down moving slider, and the rotation drive mechanism is configured to drive the magnetic field focusing head to rotate.

8. The primary magnetic field device according to claim 6 or 7, characterized in that, One or more magnetic field focusing heads are provided on the up-and-down movable slider.

9. A biomolecule extraction device, characterized in that, include: Container, magnetic bead, secondary magnet, and primary magnetic field device according to any one of claims 6-8; wherein, The container is provided with multiple receiving holes, and a connecting channel is provided between each pair of adjacent receiving holes; The magnetic beads are used to be placed in the receiving pore to adsorb biomolecules in the solution in the receiving pore; The magnetic field focusing head of the primary magnetic field device is movably disposed on the outside of the container; The secondary magnet is placed in the receiving hole. The magnetic field focusing head is configured such that when the magnetic poles of the electromagnet are in the same direction as the magnetic poles of the permanent magnet, the secondary magnet is magnetized to attract the secondary magnet; when the magnetic poles of the electromagnet are in opposite directions to the magnetic poles of the permanent magnet, the secondary magnet is demagnetized to move the secondary magnet away from the magnetic field focusing head. In this process, after the magnetic bead adsorbs biomolecules in the solution in one of the receiving holes, the secondary magnet in the receiving hole is magnetized by the magnetic field focusing head and can attract the magnetic bead. The magnetic field focusing head can move the secondary magnet with the attracted magnetic bead into another receiving hole by moving it. After the magnetic field focusing head demagnetizes the secondary magnet, the magnetic bead is dispersed in the solution in the receiving hole.

10. The biomolecule extraction device according to claim 9, characterized in that, The magnetic pole direction of the magnetic field focusing head is parallel to the hole wall it is attached to.

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