3D printed microfluidic test strip

Abstract

The utility model relates to a test paper technical field discloses a 3D printing micro -fluidic test paper, including through 3D printing and the transparent printing test paper body of shape, be equipped with several liquid setting groove and several detection groove in the printing test paper body, the printing test paper body one side is equipped with several and detection groove adaptation's waste outlet, the printing test paper body is equipped with several and liquid setting groove adaptation's liquid injection port in the side away from waste outlet, the opposite side of printing test paper body is equipped with first through -slot, second through -slot and third through -slot and is equipped with the first mobile sealing seat of having the sealing effect in the first through -slot slidingly connected with several, every liquid setting groove is equipped with the micro -channel of intercommunication between detection groove, the utility model discloses, can to several different medium of detecting simultaneously detect, and can to the medium of detecting and one or more detection mixed agent carry out the mixed reaction detection, be convenient for to the medium of detecting and detect, improve the detection efficiency of the medium of detecting.

Description

Technical Field

[0001] This utility model specifically relates to a 3D printed microfluidic test paper. Background Technology

[0002] Microfluidic test strips are an emerging technology for manipulating and detecting chemical or biological samples, primarily for detecting liquid samples.

[0003] A search revealed a centrifugal microfluidic test strip chip, such as patent publication number CN209772148U, which discloses a centrifugal microfluidic test strip chip comprising: a cover layer, a channel layer, and a base layer; the cover layer has a viewing window, a sample inlet for the test liquid, a cleaning liquid inlet, a vent, and a cover chip fixing hole; the base layer has a chromatography test strip and a base chip fixing hole, wherein the chromatography test strip consists of a sample pad, a nitrocellulose membrane, a conjugate pad, and an absorbent pad, with the nitrocellulose membrane located in the middle, and the sample pad and absorbent pad located at both ends; the conjugate pad is located between the sample pad and the nitrocellulose membrane, and a marker is provided in the conjugate pad; a detection line and a control line are provided on the nitrocellulose membrane; the channel layer has a detection area through-hole, a cleaning liquid pool, a cleaning liquid channel, a test liquid pool, a test liquid channel, a sample inlet, a vent, and a channel chip fixing hole. This utility model provides a clean, hygienic, accurate, and highly automated centrifugal microfluidic test strip chip.

[0004] The aforementioned patents have significant beneficial effects, but in practical application, they still have the following shortcomings:

[0005] Currently, there are numerous tests required for human secretions or blood. For example, the detection of calprotectin in body fluids to reflect the state of inflammation sometimes requires the addition of mixed reagents for reaction detection. However, the aforementioned comparative documents can only detect one type of test medium at a time, which is inconvenient when testing different items and reduces the detection efficiency of the test medium. Therefore, there is an urgent need in the field to improve microfluidic test strips to overcome the shortcomings of the existing technology. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a 3D-printed microfluidic test strip, which facilitates the detection of the medium to be tested and improves the detection efficiency of the medium to be tested.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a 3D-printed microfluidic test strip, comprising a transparent 3D-printed test strip body, the test strip body having a plurality of liquid-filling grooves and a plurality of detection grooves, a plurality of waste outlets adapted to the detection grooves on one side of the test strip body, a plurality of liquid-filling ports adapted to the liquid-filling grooves on the side of the test strip body away from the waste outlets, and a first through groove, a second through groove and a third through groove penetrating on opposite sides of the test strip body, with a communicating microchannel between each liquid-filling groove and the detection groove.

[0008] Preferably, a plurality of first movable sealing seats with sealing effect are slidably connected in the first through groove, and the first movable sealing seats have first connecting holes adapted to the microchannels through their opposite sides. A second movable sealing seat with sealing effect is slidably connected in the second through groove and is used to connect a plurality of microchannels. A third movable sealing seat with sealing effect is slidably connected in the third through groove and is connected to the waste discharge port.

[0009] Preferably, both the first and second movable sealing seats have exhaust chambers adapted to the microchannel. The inner sidewall of the exhaust chamber is provided with two gas-liquid separation membranes adapted to the microchannel. The two gas-liquid separation membranes are arc-shaped and the minimum distance between them is less than the diameter of the first connecting hole. The opposite sides of the first and second movable sealing seats are provided with exhaust holes that communicate with the exhaust chambers.

[0010] Preferably, the second movable sealing seat has several second connecting holes adapted to the microchannel on its side, and a third connecting hole is provided between the several second connecting holes.

[0011] Preferably, the first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are provided with sealing rings adapted to the microchannel or waste outlet on opposite sides, and sealing strips are provided on opposite sides of the first movable sealing seat, the second movable sealing seat, and the third movable sealing seat. Both the sealing rings and the sealing strips are elastic.

[0012] Preferably, the first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with a first snap-fit ​​groove on opposite sides, and one side of the sealing ring is adapted to the first snap-fit ​​groove.

[0013] Preferably, the first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with second snap-fit ​​grooves on opposite sides, and the sealing strip is adapted to the second snap-fit ​​grooves.

[0014] Preferably, a plurality of movable slots are provided on one side of the printing test paper body, and the plurality of movable slots are respectively connected to the first through slot, the second through slot and the third through slot. The opposite sides of the first movable sealing seat, the second movable sealing seat and the third movable sealing seat are threaded with movable columns that are adapted to the movable slots.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] This invention can simultaneously detect several different media to be tested, and can also perform mixed reaction detection of the media to be tested with one or more detection agents, which facilitates the detection of the media to be tested and improves the detection efficiency. By setting a gas-liquid separation membrane in the exhaust chamber, the gas contained in the media to be tested is discharged, ensuring that the media to be tested and the detection agent entering the detection tank are pure liquids, avoiding interference from gas with the detection results and improving the accuracy of the detection. Attached Figure Description

[0017] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a partial cross-sectional structural diagram of the printed test paper body in this utility model;

[0020] Figure 3 This is a schematic cross-sectional view of the present invention.

[0021] Figure 4 This is a schematic diagram of the structure of the first movable sealing seat in this utility model;

[0022] Figure 5 for Figure 3 Enlarged structural diagram at point A in the middle;

[0023] Figure 6 for Figure 4 Enlarged structural diagram at point B;

[0024] Figure 7 for Figure 4 Enlarged structural diagram at point C.

[0025] In the diagram: 1. Printing test paper body; 2. Liquid tank; 3. Detection tank; 4. Waste outlet; 5. Liquid inlet; 6. First through groove; 7. Microchannel; 8. First movable sealing seat; 9. First connecting hole; 10. Second through groove; 11. Second movable sealing seat; 12. Third through groove; 13. Exhaust chamber; 14. Gas-liquid separation membrane; 15. Exhaust hole; 16. Second connecting hole; 17. Third connecting hole; 18. Sealing ring; 19. First snap-fit ​​groove; 20. Sealing strip; 21. Second snap-fit ​​groove; 22. Movable slot; 23. Movable column; 24. Third movable sealing seat. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figures 1-7 A 3D-printed microfluidic test strip includes a transparent 3D-printed test strip body 1. The test strip body 1 has several liquid-filling slots 2 and several detection slots 3. One side of the test strip body 1 has several waste outlets 4 adapted to the detection slots 3. The side of the test strip body 1 away from the waste outlets 4 has several liquid-filling ports 5 adapted to the liquid-filling slots 2. A first through-channel 6, a second through-channel 10, and a third through-channel 12 are provided through opposite sides of the test strip body 1. Each liquid-filling slot 2 and detection slot 3 is connected by a microchannel 7. Several microchannels with... The first movable sealing seat 8 has a sealing effect. The first movable sealing seat 8 has a first connecting hole 9 that is adapted to the microchannel 7 through its opposite side. The second movable sealing seat 11 with a sealing effect is slidably connected in the second through groove 10. The second movable sealing seat 11 is used to connect several microchannels 7. The third movable sealing seat 24 with a sealing effect is slidably connected in the third through groove 12. The third movable sealing seat 24 is connected to the waste outlet 4. The second movable sealing seat 11 has several second connecting holes 16 adapted to the microchannel 7 on its side. The several second connecting holes 16 are connected by a third connecting hole 17.

[0028] By incorporating multiple liquid-holding slots 2 and detection slots 3 within the printed test strip body 1, and utilizing microchannels 7 to achieve precise liquid flow and distribution, the microchannels 7 between each liquid-holding slot 2 and detection slot 3 ensure uniform liquid flow, improving the accuracy of experimental results. This design employs multiple movable sealing seats (such as a first movable sealing seat 8, a second movable sealing seat 11, and a third movable sealing seat 24) to ensure no liquid leakage during flow, significantly enhancing the test strip's sealing performance. This design reduces liquid waste and improves experimental precision.

[0029] A waste discharge port 4, adapted to the detection groove 3, is provided on one side of the printed test paper body 1, while a liquid injection port 5, adapted to the liquid placement groove 2, is provided on the other side. This design makes the discharge of waste liquid and the injection of new liquid more convenient, simplifying the operation process. The design of the first through groove 6, the second through groove 10, and the third through groove 12 makes the liquid flow more orderly. Each through groove is equipped with a movable sealing seat with a sealing effect, ensuring precise control of the liquid flow in the channel. In addition, the connection design between the through groove and the microchannel 7 further enhances the stability of fluid control.

[0030] The printed test strip body 1 is made of transparent material, allowing users to observe the liquid flow and microchannel changes in real time. This transparent design enhances the visibility of the operation process, facilitating real-time monitoring and adjustment of experimental conditions. Each movable sealing seat is equipped with connecting holes (such as the first connecting hole 9, the second connecting hole 16, and the third connecting hole 17) adapted to the microchannel 7, ensuring smooth liquid flow through the connections between the various channels and the microchannels. This design guarantees the stability of liquid flow and reduces any unnecessary obstruction. The use of 3D printing technology to manufacture the test strips allows the microfluidic system to be customized to meet specific needs, enabling the design of complex microchannels and structures, and rapid adjustment and optimization under different experimental requirements.

[0031] Specifically, a suitable 3D printing material is selected, such as polylactic acid (PLA) material with good biocompatibility and chemical stability, to print the test paper body 1. The test paper body 1 is transparent, allowing observation of the flow of the medium to be tested. The medium to be tested is injected into the liquid tank 2 through the injection port 5. There are several injection ports 5, with several injection ports 5 on both sides, mainly determined by the placement position of the test paper body 1. Unused injection ports 5 can be blocked with rubber plugs. A negative pressure generating device can be installed at the injection port 5 or the waste outlet 4 to pressurize and transport the medium to be tested. Several liquid tanks 2 can be filled with different media to be tested simultaneously, or the medium to be tested can be filled in one liquid tank 2 while the other liquid tanks 2 are filled with a compatible test mixture.

[0032] When testing the medium to be tested in a single liquid tank 2, the position of the first movable sealing seat 8 in the first channel 6 is adjusted so that the first connecting hole 9 on the first movable sealing seat 8 is aligned with the position of the microchannel 7, and the first movable sealing seat 8 is sealed within the first channel 6 and the microchannel 7. The first channel 6 and the second channel 10 are of the same size. At this time, several first movable sealing seats 8 are also installed in the second channel 10, and the first connecting hole 9 of the first movable sealing seat 8 in the second channel 10 is aligned with the position of the microchannel 7 by moving the first movable sealing seat 8 in the second channel 10. The third movable sealing seat 24 in the third channel 12 is moved, and the first movable sealing seat 24 in the third channel 12 is moved. The position of the three movable sealing seat 24 is aligned with the positions of the waste outlet 4 and the microchannel 7. The medium to be tested in the liquid tank 2 is driven by an external negative pressure device to flow through the first connecting hole 9 and the microchannel 7 into the detection tank 3 for detection. The shape of the microchannel 7 can be straight or curved. When the medium to be tested flows to the position of the liquid tank 2, the third movable sealing seat 24 located in the third channel 12 moves to seal the position of the waste outlet 4. After waiting for the reaction time, the color in the detection tank 3 is observed to distinguish the media. Different media to be tested can be injected into each liquid tank 2, so that multiple media to be tested can be detected at the same time.

[0033] When it is necessary to perform a reaction test on the medium to be tested with different test agents, the first movable sealing seat 8 in the second through groove 10 is taken out, and the appropriate second movable sealing seat 11 is installed according to the number of test agents to be mixed. The number of second connecting holes 16 on the second movable sealing seat 11 is equal to the number of test agents plus the medium to be tested.

[0034] Figure 5The diagram shows a method of mixing a detection agent with a test medium for detection. The test medium and the detection agent are injected into several liquid tanks 2. The position of the second movable sealing seat 11 in the third channel 12 is adjusted. Several second connecting holes 16 on one side of the second movable sealing seat 11 are aligned with the positions of corresponding microchannels 7. Another second connecting hole 16 is aligned with one of the microchannels 7 connected to the detection tank 3. An external negative pressure device drives the test medium to move. Several detection agents and the test medium pass through the microchannels 7, the first connecting hole 9, and the third connecting hole 17, and then through the second connecting hole 1... The liquid flows into a detection tank 3 to perform a mixing reaction detection of the medium to be tested with one or more detection agents. Subsequently, the waste liquid is discharged through the third movable sealing seat 24 in the third channel 12, which is connected to the microchannel 7 and the waste outlet 4. Through the movable first movable sealing seat 8 and the movable or replaceable second movable sealing seat 11, several different media to be tested can be tested simultaneously, and the medium to be tested can be mixed with one or more detection agents for reaction detection, which facilitates the testing of the medium to be tested and improves the testing efficiency.

[0035] As a technical optimization of this utility model, both the first movable sealing seat 8 and the second movable sealing seat 11 are provided with exhaust chambers 13 adapted to the microchannel 7. The inner sidewall of the exhaust chamber 13 is provided with two gas-liquid separation membranes 14 adapted to the microchannel 7. The two gas-liquid separation membranes 14 are arc-shaped and the minimum distance between them is less than the diameter of the first connecting hole 9. The opposite sides of the first movable sealing seat 8 and the second movable sealing seat 11 are provided with exhaust holes 15 that communicate with the exhaust chambers 13.

[0036] An exhaust chamber 13 is formed within the first movable sealing seat 8 and the second movable sealing seat 11, and two gas-liquid separation membranes 14 are provided to effectively separate air bubbles from the liquid. The arc-shaped design and small spacing of the gas-liquid separation membranes improve the efficiency of gas-liquid separation, prevent air bubbles from interfering with the flow of the liquid, and ensure a more stable and uniform flow of the liquid in the microfluidic system. Through the gas-liquid separation membranes 14, excess air bubbles in the liquid are isolated, thereby preventing air bubbles from entering the microchannel 7. This ensures that the liquid is not disturbed by air bubbles during flow, reduces flow instability, and improves the accuracy and repeatability of the detection results.

[0037] The vent holes 15 on the opposite sides of the first movable sealing seat 8 and the second movable sealing seat 11 allow the exhaust chamber 13 to be effectively connected to the external environment, ensuring that the gas can be discharged quickly and completely. This not only avoids the problem of gas accumulation, but also improves the overall system flow efficiency. Since the gas-liquid separation membrane 14 can effectively prevent gas from entering the microchannel, it avoids the contamination and blockage of the fluid in the microchannel by air bubbles, keeps the microchannel clean, and ensures more precise experimental operation.

[0038] The design of the exhaust chamber 13 improves the overall sealing effect. Through an effective gas-liquid separation mechanism, the liquid flow process is made more stable, while avoiding any potential leakage problems. This scheme effectively avoids the influence of air bubbles and abnormal fluctuations in liquid flow through the design of the gas-liquid separation membrane and exhaust port, thereby improving the reliability and long-term stability of the entire microfluidic system. This optimized scheme can adapt to different liquid flow and gas emission requirements, especially in microfluidic experiments that require high precision and high stability, providing more flexible and efficient support.

[0039] Specifically, the gas-liquid separation membrane 14 is a known existing technology, and the two gas-liquid separation membranes 14 are arc-shaped with a minimum distance between them smaller than the aperture of the first connecting hole 9. When the medium to be tested passes between the two gas-liquid separation membranes 14, it is squeezed, and the gas contained in the medium to be tested enters the exhaust chamber 13 through the gas-liquid separation membrane 14 and is discharged through the exhaust hole 15, thereby venting the gas contained in the medium to be tested and ensuring that the medium to be tested and the test mixture entering the test tank 3 are pure liquids, avoiding interference from gas with the test results and improving the accuracy of the test.

[0040] As a technical optimization of this utility model, the first movable sealing seat 8, the second movable sealing seat 11, and the third movable sealing seat 24 are all provided with sealing rings 18 that are adapted to the microchannel 7 or the waste outlet 4 on opposite sides. The first movable sealing seat 8, the second movable sealing seat 11, and the third movable sealing seat 24 are all provided with sealing strips 20 on opposite sides. Both the sealing rings 18 and the sealing strips 20 are elastic. The first movable sealing seat 8, the second movable sealing seat 11, and the third movable sealing seat 24 are all provided with first snap-fit ​​grooves 19 on opposite sides. One side of the sealing ring 18 is adapted to the first snap-fit ​​groove 19. The first movable sealing seat 8, the second movable sealing seat 11, and the third movable sealing seat 24 are all provided with second snap-fit ​​grooves 21 on opposite sides. The sealing strips 20 are adapted to the second snap-fit ​​grooves 21, thereby achieving the sealing effect between the first movable sealing seat 8 and the first through groove 6, the sealing effect between the second movable sealing seat 11 and the second through groove 10, and the sealing effect between the third through groove 12 and the third movable sealing seat 24.

[0041] By providing sealing rings 18 and sealing strips 20 on the opposite sides of the first, second, and third movable sealing seats, the system's sealing performance can be effectively improved. These sealing rings and strips are elastic, maintaining good sealing performance under different pressure and temperature conditions, preventing liquid leakage or gas infiltration, and ensuring a stable internal environment for the system. The matching design of the sealing rings 18 and 20 with the corresponding snap-fit ​​grooves 19 and 21 ensures that the sealing rings and strips can firmly engage with the sealing seats and through grooves. The presence of the snap-fit ​​grooves further ensures stable positioning of the sealing components during assembly, preventing loosening or misalignment of components, thereby ensuring consistent and reliable sealing performance.

[0042] This solution achieves efficient sealing between the first, second, and third through channels and their corresponding movable sealing seats through the cooperation of sealing rings and sealing strips between each channel and the movable sealing seat. Both the microchannel 7 and the waste outlet 4 achieve precise and robust seals, preventing fluid leakage or environmental pollution due to poor sealing. The sealing rings and sealing strips are elastic, adapting to minor deformations that may occur during long-term use, preventing seal failure due to material aging or deformation. The overall structural design ensures that the sealing components maintain excellent sealing performance even under external forces, thereby improving the system's durability.

[0043] A well-designed seal reduces system leakage, thus lowering maintenance frequency and costs. Furthermore, the sealing components are designed for ease of installation and replacement, effectively minimizing downtime after system failures and improving equipment reliability and availability. The flexible design of the sealing rings and strips allows the system to adapt to various operating environments (such as different pressures, temperatures, and fluid media), enhancing its flexibility and adaptability. The system maintains excellent sealing performance even under high temperature and high pressure conditions, meeting diverse operational needs. Effective sealing prevents liquid or gas leakage, which is crucial for preventing environmental pollution, protecting internal components from damage, and improving operator safety. Improved sealing performance ensures no accidental leaks occur during operation, enhancing system safety. At the application level, the 3D-printed microfluidic test strip of this embodiment can adopt the above-mentioned sealed design, design an independent sample pretreatment chamber and detection channel, and adapt to different body fluids through detachable modules (such as blood module with anticoagulant membrane, feces module with filter layer), thereby detecting calprotectin in body fluids, which can be used to provide feedback on human infectious and inflammatory diseases. It is suitable for early screening and type identification of such diseases, and provides an efficient and low-cost bedside solution for efficacy monitoring.

[0044] As a technical optimization of this utility model, a plurality of movable slots 22 are provided on one side of the printing test paper body 1. The plurality of movable slots 22 are respectively connected to the first through slot 6, the second through slot 10 and the third through slot 12. The first movable sealing seat 8, the second movable sealing seat 11 and the third movable sealing seat 24 are threadedly connected to the opposite sides of the movable slots 22 with movable posts 23 adapted to the movable slots 22. The movable posts 23 are threadedly connected to the side of the first movable sealing seat 8, the second movable sealing seat 11 or the third movable sealing seat 24, and the first movable sealing seat 8, the second movable sealing seat 11 or the third movable sealing seat 24 are displaced by the movable posts 23.

[0045] By introducing a threaded movable column 23 between the movable slot 22 and the first, second, and third movable sealing seats, precise adjustment of the sealing seat position can be achieved. The movable column can adjust the specific position of the sealing seat according to requirements, ensuring optimal fit of the sealing seat under different working conditions, thereby improving overall sealing performance. By using the threaded movable column 23, the displacement of the sealing seat can be flexibly adjusted according to actual needs, allowing the sealing effect between the first, second, and third through slots and the movable sealing seats to be optimized according to different working conditions. This adjustability allows the system to maintain good sealing performance when facing different working pressures or media.

[0046] This design allows the movable column to be adjusted via a threaded connection, simplifying the installation and disassembly of the movable seal seat. During system maintenance, operators can easily adjust the position of the seal seat, even without completely disassembling the equipment, enabling quick repairs and adjustments, reducing downtime and maintenance costs. The movable column provides a stable positioning mechanism, ensuring the seal seat remains in the required position, thus preventing seal failure due to seal seat misalignment. This design significantly improves the stability and reliability of the system during long-term operation.

[0047] By precisely adjusting the position of the sealing seat, it can better adapt to different working conditions and achieve accurate sealing connection. This optimization can prevent problems such as leakage and seepage, ensuring that the system's sealing effect is always at its best and avoiding liquid or gas leakage.

[0048] Finally, it should be noted that in the description of this utility model, the terms "vertical," "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0049] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0050] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A 3D-printed microfluidic test strip, characterized in that, The test paper body is 3D printed and is transparent. The test paper body has several liquid inlets and several detection slots. One side of the test paper body has several waste outlets that are adapted to the detection slots. The side of the test paper body away from the waste outlets has several liquid inlets that are adapted to the liquid inlets. The opposite sides of the test paper body have a first through groove, a second through groove and a third through groove. Each liquid inlet and detection slot is connected by a microchannel.

2. The 3D-printed microfluidic test paper according to claim 1, characterized in that, Several first movable sealing seats with sealing effect are slidably connected in the first through groove. The first movable sealing seats have first connecting holes adapted to the microchannels through their opposite sides. A second movable sealing seat with sealing effect is slidably connected in the second through groove. The second movable sealing seat is used to connect several microchannels. A third movable sealing seat with sealing effect is slidably connected in the third through groove. The third movable sealing seat is connected to the waste discharge port.

3. The 3D-printed microfluidic test paper according to claim 2, characterized in that, Both the first and second movable sealing seats have exhaust chambers adapted to the microchannel. The inner sidewall of the exhaust chamber is provided with two gas-liquid separation membranes adapted to the microchannel. The two gas-liquid separation membranes are arc-shaped and the minimum distance between them is less than the diameter of the first connecting hole. The opposite sides of the first and second movable sealing seats are provided with exhaust holes that communicate with the exhaust chambers.

4. The 3D-printed microfluidic test paper according to claim 3, characterized in that, The second movable sealing seat has several second connecting holes adapted to the microchannel on its side, and a third connecting hole connecting the several second connecting holes is provided between them.

5. A 3D-printed microfluidic test paper according to claim 2, characterized in that, The first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with sealing rings on opposite sides that are adapted to the microchannel or the waste outlet. The first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with sealing strips on opposite sides. Both the sealing rings and the sealing strips are elastic.

6. The 3D-printed microfluidic test paper according to claim 5, characterized in that, The first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with a first snap-fit ​​groove on opposite sides, and one side of the sealing ring is adapted to the first snap-fit ​​groove.

7. A 3D-printed microfluidic test strip according to claim 5, characterized in that, The first movable sealing seat, the second movable sealing seat, and the third movable sealing seat are all provided with second snap-fit ​​grooves on opposite sides, and the sealing strip is adapted to the second snap-fit ​​grooves.

8. A 3D-printed microfluidic test paper according to claim 2, characterized in that, The printing test paper body has several movable slots on one side, which are respectively connected to the first through slot, the second through slot and the third through slot. The first movable sealing seat, the second movable sealing seat and the third movable sealing seat are threaded to the opposite sides of the movable column that is adapted to the movable slot.

Images