Cat fever virus nucleic acid qPCR detection sample pretreatment box

Abstract

The utility model relates to cat distemper virus nucleic acid qPCR detection sample pretreatment box technical field discloses a cat distemper virus nucleic acid qPCR detection sample pretreatment box, including the pretreatment box casing, the pretreatment box casing upper end is provided with qPCR test tube placing plate, qPCR test tube placing plate top is provided with a plurality of groups of clamping components, the four corners below of qPCR test tube placing plate all are provided with telescopic components, the middle part below of qPCR test tube placing plate is provided with push rod, the push rod below is connected with cam and rolls, the middle part fixed connection of cam has rotation axis, the one end of rotation axis is connected with motor, and the other end of rotation axis rotationally connects the inner wall of pretreatment box casing. The utility model has realized the quick lysis of cat distemper virus sample, nucleic acid release, sample purification, and adapts qPCR detection, improves positive detection rate and experimental efficiency simultaneously.

Description

Technical Field

[0001] This utility model relates to the technical field of feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit, specifically a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit. Background Technology

[0002] Feline panleukopenia (FPV) is widespread among felines, especially kittens, with a consistently high mortality rate. The main symptoms are fever, vomiting, and diarrhea, similar to other gastrointestinal diseases, making accurate diagnosis difficult based solely on clinical manifestations. Currently, various methods exist for the clinical diagnosis of FPV infection, such as electron microscopy, virus isolation, latex agglutination, hemagglutination, ELISA, and PCR analysis. Among these, quantitative real-time PCR (qPCR) is a simple and convenient method, allowing for FPV detection without electrophoresis after amplification, and has become an important tool for FPV detection. However, in the qPCR detection process, slow lysis and nucleic acid release of FPV samples, along with low sample purification, interfere with subsequent nucleic acid purification steps and may even inhibit the qPCR reaction, leading to a lower positive detection rate and inaccurate experimental results. Utility Model Content

[0003] The purpose of this invention is to address the shortcomings of existing technologies by proposing a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment box, comprising a pretreatment box shell, a qPCR tube placement plate provided at the upper end of the pretreatment box shell, a plurality of clamping components provided above the qPCR tube placement plate, telescopic components provided at the lower four corners of the qPCR tube placement plate, a push rod provided at the lower center of the qPCR tube placement plate, a cam rotatably connected below the push rod, a rotating shaft fixedly connected to the middle of the cam, a motor connected to one end of the rotating shaft, and the other end of the rotating shaft rotatably connected to the inner wall of the pretreatment box shell.

[0005] As a further description of the above technical solution:

[0006] The clamping components are evenly distributed above the qPCR tube placement plate. Each clamping component includes a clamping block 1, a clamping spring 1 on one side of the clamping block 1, and a clamping block 2 symmetrically arranged on the side of the clamping block 1 away from the clamping spring 1. The qPCR tube is clamped between the clamping block 1 and the clamping block 2. A clamping spring 2 is arranged on the side of the clamping block 2 away from the qPCR tube.

[0007] As a further description of the above technical solution:

[0008] One end of the clamping spring is fixedly connected to the outer side of the clamping block, and the other end of the clamping spring is fixedly connected to the inner wall of the qPCR tube placement plate. One end of the clamping spring is fixedly connected to the outer side of the clamping block, and the other end of the clamping spring is fixedly connected to the inner wall of the qPCR tube placement plate.

[0009] As a further description of the above technical solution:

[0010] The qPCR tube placement plate is slidably connected to the grooves on both sides of the upper inner wall of the pretreatment box shell by sliders fixed at both ends.

[0011] As a further description of the above technical solution:

[0012] The telescopic assembly includes a base, a telescopic rod is provided above the base, a telescopic spring is sleeved on the telescopic rod, the lower end of the telescopic spring is fixedly provided above the base, and the upper end of the telescopic spring is fixedly connected to the lower part of the qPCR tube placement plate.

[0013] As a further description of the above technical solution:

[0014] The push rod passes through and is slidably connected to the fixing plate, which is fixedly connected to the inner wall of the pretreatment box housing.

[0015] As a further description of the above technical solution:

[0016] A reset spring is fitted on the push rod. The upper end of the reset spring is fixedly connected to the bottom of the fixing plate, and the lower end of the reset spring is fixedly connected to the top of the fixing block set at the lower end of the push rod.

[0017] This utility model has the following beneficial effects:

[0018] 1. The automatic reciprocating oscillation of the qPCR test tube placement plate is achieved by driving the cam, push rod and telescopic components with a motor. This can quickly lyse feline panleukopenia virus samples, accelerate nucleic acid release and purification, and significantly shorten the pretreatment time. At the same time, multiple sets of clamping components can be used to process batches of samples simultaneously, which can significantly improve experimental efficiency and is compatible with qPCR detection procedures.

[0019] 2. The clamping structure prevents the test tube from falling off during shaking, ensuring the stability of sample processing; sufficient shaking can reduce false negatives caused by insufficient nucleic acid release or interference from impurities, effectively improving the positive detection rate and providing a reliable sample basis for the accurate detection of feline panleukopenia virus. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit proposed in this utility model;

[0021] Figure 2This is a schematic diagram of the qPCR tube placement plate of a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit proposed in this utility model;

[0022] Figure 3 This is a cross-sectional view of a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit proposed in this utility model;

[0023] Figure 4 This invention provides a sample pretreatment kit for feline panleukopenia virus nucleic acid qPCR detection. Figure 1 Enlarged view of point A.

[0024] Legend:

[0025] 1. Pretreatment box shell; 2. qPCR tube placement plate; 3. Clamping assembly; 31. Clamping block one; 32. Clamping block two; 33. Clamping spring one; 34. Clamping spring two; 4. Slider; 5. Slide groove; 6. Telescopic assembly; 61. Base; 62. Telescopic rod; 63. Telescopic spring; 7. Motor; 8. Rotating shaft; 9. Fixing plate; 10. Cam; 11. Push rod; 12. Return spring; 13. qPCR tube. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. The utility model will be further described in detail below with reference to the accompanying drawings.

[0028] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0029] Example 1:

[0030] like Figures 1 to 4 As shown, this embodiment provides a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit, including a pretreatment kit shell 1. A qPCR tube placement plate 2 is provided on the upper end of the pretreatment kit shell 1. Several sets of clamping components 3 are provided above the qPCR tube placement plate 2. Telescopic components 6 are provided at the lower four corners of the qPCR tube placement plate 2. A push rod 11 is provided at the lower center of the qPCR tube placement plate 2. A cam 10 is rotatably connected below the push rod 11. A rotating shaft 8 is fixedly connected to the middle of the cam 10. One end of the rotating shaft 8 is connected to a motor 7, and the other end of the rotating shaft 8 is rotatably connected to the inner wall of the pretreatment kit shell 1.

[0031] In this embodiment, the clamping component, the telescopic component, and the cam constitute a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit according to this application.

[0032] Understandable, Figure 1 This illustration only shows some components of a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit. The actual shape, size, location, and structure of these components are not subject to change. Figure 1 Due to limitations, a feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit can also include, compared to... Figure 1 More or fewer parts.

[0033] In this embodiment, the pretreatment box shell 1 provides a supporting frame for the entire device, and the qPCR tube placement plate 2 is used to place the qPCR tubes 13 to be processed. Simultaneously, the telescopic components 6 at the four corners of the qPCR tube placement plate 2 act as buffers and supports. The motor 7 drives the rotating shaft 8 to rotate the cam 10. The cam, through rolling contact with the push rod 11, pushes the qPCR tube placement plate 2 to reciprocate up and down, achieving agitation pretreatment of the samples within the qPCR tubes 13. The clamping component 3 fixes the qPCR tubes 13 during movement, preventing them from shaking or falling. Mechanical agitation replaces manual shaking, achieving standardized sample pretreatment and improving the consistency of feline panleukopenia virus nucleic acid qPCR detection. Automated operation reduces manual intervention and the risk of sample contamination, making it suitable for batch sample processing scenarios.

[0034] Specifically, clamping components 3 are evenly distributed above the qPCR tube placement plate 2. The clamping components 3 include clamping block 31, clamping spring 33 on one side of clamping block 31, clamping block 32 symmetrically arranged on the side of clamping block 31 away from clamping spring 33, clamping qPCR tube 13 between clamping block 31 and clamping block 32, and clamping spring 34 on the side of clamping block 32 away from qPCR tube 13.

[0035] In this embodiment, in the clamping assembly 3, clamping block 1 31 and clamping block 2 32 clamp the qPCR tube 13 from both sides under the elastic force of clamping spring 1 33 and clamping spring 2 34. The elastic deformation of the springs can adapt to the diameter of qPCR tubes 13 of different specifications, ensuring a stable clamping without damaging the qPCR tubes 13. The symmetrically distributed clamping blocks, in conjunction with the springs, can firmly fix the qPCR tubes 13 and buffer the impact force during the shaking process, preventing the tubes from breaking or the samples from spilling, thus ensuring the safety of the pretreatment process. At the same time, clamping block 1 31 and clamping block 2 32 are provided with a rubber layer on the side closest to the qPCR tubes 13 to prevent the clamping assembly 3 from damaging the tubes.

[0036] Specifically, one end of clamping spring 33 is fixedly connected to the outside of clamping block 31, and the other end of clamping spring 33 is fixedly connected to the inner wall of qPCR tube placement plate 2. One end of clamping spring 34 is fixedly connected to the outside of clamping block 32, and the other end of clamping spring 34 is fixedly connected to the inner wall of qPCR tube placement plate 2.

[0037] In a preferred embodiment, clamping spring 33 and clamping spring 34 are respectively fixed between the clamping block and the inner wall of the qPCR tube placement plate 2. When the qPCR tube 13 is placed in or removed, the springs are compressed or stretched, generating a reverse elastic force to drive the clamping block to reset, thus achieving automatic clamping or releasing of the qPCR tube 13. The fixed connection method of the springs ensures stable clamping force, allowing for adaptation to different qPCR tubes 13 without manual adjustment, simplifying the operation process and improving the efficiency of sample replacement.

[0038] Example 2:

[0039] Specifically, the qPCR tube placement plate 2 is slidably connected to the grooves 5 set on both sides of the upper inner wall of the pretreatment box shell 1 through the sliders 4 fixed at both ends.

[0040] In this embodiment, the sliders 4 at both ends of the qPCR tube placement plate 2 are embedded in the grooves 5 of the pretreatment box housing 1. When the qPCR tube placement plate 2 moves up and down under the action of the cam 10 and the telescopic component 6, the sliders 4 slide along the grooves 5, limiting the movement trajectory of the qPCR tube placement plate 2 and preventing it from deviating or tilting. The cooperation between the sliders 4 and the grooves 5 ensures that the qPCR tube placement plate 2 moves up and down smoothly, avoiding collisions caused by shaking of the qPCR tubes 13 during the oscillation process, while ensuring that the oscillation amplitude of each qPCR tube 13 is consistent, thus improving the uniformity of sample pretreatment.

[0041] Specifically, the telescopic component 6 includes a base 61, a telescopic rod 62 is provided above the base 61, a telescopic spring 63 is sleeved on the telescopic rod 62, the lower end of the telescopic spring 63 is fixedly provided above the base 61, and the upper end of the telescopic spring 63 is fixedly connected to the lower part of the qPCR tube placement plate 2.

[0042] With this configuration, the base 61 of the telescopic component 6 is fixed to the bottom of the pretreatment box housing 1. The telescopic rod 62 extends and retracts with the qPCR tube placement plate 2. The telescopic spring 63, sleeved on the telescopic rod 62, is compressed when the qPCR tube placement plate 2 rises, storing elastic potential energy. When it descends, it releases the potential energy to assist in resetting and buffering oscillation impact. The telescopic spring 63 cooperates with the cam mechanism to make the up-and-down movement of the qPCR tube placement plate 2 smoother and reduce mechanical wear; at the same time, it provides stable support for the qPCR tube placement plate 2, preventing it from sagging due to gravity and ensuring precise and controllable oscillation amplitude.

[0043] Example 3:

[0044] Specifically, the push rod 11 passes through and slides through the fixing plate 9, and the fixing plate 9 is fixedly connected to the inner wall of the pretreatment box housing 1.

[0045] The push rod 11 passes through the fixed plate 9 and can slide along it. The fixed plate 9 is fixed to the inner wall of the pretreatment box housing 1, providing a guiding function for the push rod 11, ensuring that the push rod 11 always moves in the vertical direction, accurately transmitting the driving force of the cam 10 to the qPCR tube placement plate 2, avoiding device jamming or damage caused by uneven force, and improving the reliability of mechanical transmission.

[0046] Specifically, a reset spring 12 is sleeved on the push rod 11. The upper end of the reset spring 12 is fixedly connected to the lower part of the fixing plate 9, and the lower end of the reset spring 12 is fixedly connected to the upper part of the fixing block set at the lower end of the push rod 11.

[0047] In this embodiment, the return spring 12 on the push rod 11 is stretched when the push rod 11 is lifted by the cam 10, generating a downward pulling force. When the cam 10 rotates to a non-protruding position, the pulling force of the return spring 12 drives the push rod to move downward, ensuring that the push rod 11 is always in close contact with the cam, thus ensuring continuous transmission. The return spring 12 ensures that the push rod 11 and the cam 10 have a gap-free fit, avoiding impacts or pauses during oscillation, making the up-and-down movement of the qPCR tube placement plate 2 smooth and improving the effect of sample oscillation pretreatment.

[0048] In actual use, motor 7 drives rotating shaft 8 to rotate cam 10. Cam 10 pushes qPCR tube placement plate 2 upward through rolling contact with push rod 11. At this time, the telescopic spring 63 of telescopic component 6 is compressed and the return spring 12 on push rod 11 is stretched. When cam 10 rotates to the non-protruding part, telescopic spring 63 and return spring 12 release potential energy, causing qPCR tube placement plate 2 to descend, forming up-and-down reciprocating oscillation. At the same time, the sliders 4 at both ends of qPCR tube placement plate 2 slide along the grooves 5 on the inner wall of pretreatment box shell 1 to ensure smooth movement. qPCR tubes 13 placed on qPCR tube placement plate 2 are clamped from both sides by clamping blocks driven by clamping springs in clamping component 3, remaining stable during oscillation, realizing rapid oscillation pretreatment of samples in qPCR tubes 13, accelerating feline panleukopenia virus sample lysis, nucleic acid release and purification.

[0049] It should be noted that all electrical components mentioned in this article are connected to an external main controller and 220V AC mains power. The main controller can be a conventional known device that can be controlled by a computer or other means. The detailed description of known functions and known components is omitted in the specific implementation of this disclosure. In order to ensure the compatibility of the device, the operating methods used are consistent with the parameters of commercially available instruments.

[0050] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A sample pretreatment kit for feline panleukopenia virus nucleic acid qPCR detection, characterized in that: The device includes a pretreatment box housing (1), a qPCR tube placement plate (2) is provided on the upper end of the pretreatment box housing (1), several sets of clamping components (3) are provided above the qPCR tube placement plate (2), telescopic components (6) are provided at the lower four corners of the qPCR tube placement plate (2), a push rod (11) is provided at the lower middle part of the qPCR tube placement plate (2), a cam (10) is rolled below the push rod (11), a rotating shaft (8) is fixedly connected in the middle of the cam (10), a motor (7) is connected to one end of the rotating shaft (8), and the other end of the rotating shaft (8) is rotatably connected to the inner wall of the pretreatment box housing (1); The clamping components (3) are evenly distributed above the qPCR tube placement plate (2). The clamping components (3) include a clamping block one (31), a clamping spring one (33) is provided on one side of the clamping block one (31), and a clamping block two (32) is symmetrically provided on the side of the clamping block one (31) away from the clamping spring one (33). The qPCR tube (13) is clamped between the clamping block one (31) and the clamping block two (32). A clamping spring two (34) is provided on the side of the clamping block two (32) away from the qPCR tube (13). The telescopic assembly (6) includes a base (61), a telescopic rod (62) is provided above the base (61), a telescopic spring (63) is sleeved on the telescopic rod (62), the lower end of the telescopic spring (63) is fixedly provided above the base (61), and the upper end of the telescopic spring (63) is fixedly connected to the lower part of the qPCR test tube placement plate (2). The push rod (11) passes through and is slidably connected to the fixing plate (9), and the fixing plate (9) is fixedly connected to the inner wall of the pretreatment box housing (1); A reset spring (12) is sleeved on the push rod (11). The upper end of the reset spring (12) is fixedly connected to the bottom of the fixing plate (9), and the lower end of the reset spring (12) is fixedly connected to the top of the fixing block set at the lower end of the push rod (11).

2. The feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit according to claim 1, characterized in that: One end of the clamping spring (33) is fixedly connected to the outside of the clamping block (31), and the other end of the clamping spring (33) is fixedly connected to the inner wall of the qPCR tube placement plate (2). One end of the clamping spring (34) is fixedly connected to the outside of the clamping block (32), and the other end of the clamping spring (34) is fixedly connected to the inner wall of the qPCR tube placement plate (2).

3. The feline panleukopenia virus nucleic acid qPCR detection sample pretreatment kit according to claim 1, characterized in that: The qPCR tube placement plate (2) is slidably connected to the grooves (5) set on both sides of the upper inner wall of the pretreatment box shell (1) by sliders (4) fixed at both ends.

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